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PMC:1064854 / 61017-62678 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T36774 0-3 NN denotes RNA
T36776 3-5 , denotes ,
T36777 5-8 NN denotes DNA
T36778 8-10 , denotes ,
T36779 10-13 CC denotes and
T36780 14-21 NN denotes protein
T36775 22-30 NNS denotes analyses
T36781 30-171 sentence denotes Total RNA was isolated by the RNAzol method (Tel-Test, Friendswood, Texas) and Northern blotting was performed as previously described [40].
T36782 31-36 JJ denotes Total
T36783 37-40 NN denotes RNA
T36785 41-44 VBD denotes was
T36784 45-53 VBN denotes isolated
T36786 54-56 IN denotes by
T36787 57-60 DT denotes the
T36789 61-67 NN denotes RNAzol
T36788 68-74 NN denotes method
T36790 75-76 -LRB- denotes (
T36792 76-79 NNP denotes Tel
T36793 79-80 HYPH denotes -
T36791 80-84 NNP denotes Test
T36794 84-86 , denotes ,
T36795 86-97 NNP denotes Friendswood
T36796 97-99 , denotes ,
T36797 99-104 NNP denotes Texas
T36798 104-105 -RRB- denotes )
T36799 106-109 CC denotes and
T36800 110-118 NNP denotes Northern
T36801 119-127 NN denotes blotting
T36803 128-131 VBD denotes was
T36802 132-141 VBN denotes performed
T36804 142-144 IN denotes as
T36806 145-155 RB denotes previously
T36805 156-165 VBN denotes described
T36807 166-167 -LRB- denotes [
T36808 167-169 CD denotes 40
T36809 169-170 -RRB- denotes ]
T36810 170-171 . denotes .
T36811 171-275 sentence denotes The PGC-1β and PRC cDNAs were generous gifts from Bruce Spiegelman and Richard Scarpulla, respectively.
T36812 172-175 DT denotes The
T36814 176-179 NN denotes PGC
T36816 179-180 HYPH denotes -
T36815 180-182 NN denotes 1β
T36817 183-186 CC denotes and
T36818 187-190 NN denotes PRC
T36813 191-196 NNS denotes cDNAs
T36819 197-201 VBD denotes were
T36820 202-210 JJ denotes generous
T36821 211-216 NNS denotes gifts
T36822 217-221 IN denotes from
T36823 222-227 NNP denotes Bruce
T36824 228-238 NNP denotes Spiegelman
T36825 239-242 CC denotes and
T36826 243-250 NNP denotes Richard
T36827 251-260 NNP denotes Scarpulla
T36828 260-262 , denotes ,
T36829 262-274 RB denotes respectively
T36830 274-275 . denotes .
T36831 275-323 sentence denotes The UCP-1 cDNA was a gift from Daniel Ricquier.
T36832 276-279 DT denotes The
T36834 280-283 NN denotes UCP
T36835 283-284 HYPH denotes -
T36836 284-285 CD denotes 1
T36833 286-290 NN denotes cDNA
T36837 291-294 VBD denotes was
T36838 295-296 DT denotes a
T36839 297-301 NN denotes gift
T36840 302-306 IN denotes from
T36841 307-313 NNP denotes Daniel
T36842 314-322 NNP denotes Ricquier
T36843 322-323 . denotes .
T36844 323-363 sentence denotes RT-PCR was performed as described [41].
T36845 324-326 NN denotes RT
T36847 326-327 HYPH denotes -
T36846 327-330 NN denotes PCR
T36849 331-334 VBD denotes was
T36848 335-344 VBN denotes performed
T36850 345-347 IN denotes as
T36851 348-357 VBN denotes described
T36852 358-359 -LRB- denotes [
T36853 359-361 CD denotes 41
T36854 361-362 -RRB- denotes ]
T36855 362-363 . denotes .
T36856 363-596 sentence denotes In brief, total RNA isolated from soleus muscle, BAT, and heart of 1–2-mo-old PGC-1α+/+ or PGCα−/− mice was reverse transcribed with Taqman reverse transcription reagents (Applied Biosystems, Foster City, California, United States).
T36857 364-366 IN denotes In
T36859 367-372 JJ denotes brief
T36860 372-374 , denotes ,
T36861 374-379 JJ denotes total
T36862 380-383 NN denotes RNA
T36863 384-392 VBN denotes isolated
T36864 393-397 IN denotes from
T36865 398-404 NN denotes soleus
T36866 405-411 NN denotes muscle
T36867 411-413 , denotes ,
T36868 413-416 NN denotes BAT
T36869 416-418 , denotes ,
T36870 418-421 CC denotes and
T36871 422-427 NN denotes heart
T36872 428-430 IN denotes of
T36873 431-432 CD denotes 1
T36875 432-433 SYM denotes –
T36874 433-434 CD denotes 2
T36877 434-435 HYPH denotes -
T36876 435-437 NN denotes mo
T36879 437-438 HYPH denotes -
T36878 438-441 JJ denotes old
T36881 442-445 NN denotes PGC
T36883 445-446 HYPH denotes -
T36882 446-448 NN denotes 1α
T36884 448-449 SYM denotes +
T36885 449-450 HYPH denotes /
T36886 450-451 SYM denotes +
T36887 452-454 CC denotes or
T36888 455-459 NN denotes PGCα
T36889 459-460 SYM denotes −
T36890 460-461 HYPH denotes /
T36891 461-462 SYM denotes −
T36880 463-467 NNS denotes mice
T36892 468-471 VBD denotes was
T36893 472-479 RB denotes reverse
T36858 480-491 VBN denotes transcribed
T36894 492-496 IN denotes with
T36895 497-503 NNP denotes Taqman
T36897 504-511 JJ denotes reverse
T36898 512-525 NN denotes transcription
T36896 526-534 NNS denotes reagents
T36899 535-536 -LRB- denotes (
T36901 536-543 NNP denotes Applied
T36900 544-554 NNP denotes Biosystems
T36902 554-556 , denotes ,
T36903 556-562 NNP denotes Foster
T36904 563-567 NNP denotes City
T36905 567-569 , denotes ,
T36906 569-579 NNP denotes California
T36907 579-581 , denotes ,
T36908 581-587 NNP denotes United
T36909 588-594 NNP denotes States
T36910 594-595 -RRB- denotes )
T36911 595-596 . denotes .
T36912 596-737 sentence denotes Reactions were performed in triplicate in 96-well format using Taqman core reagents and a Prism 7500 Sequence Detector (Applied Biosystems).
T36913 597-606 NNS denotes Reactions
T36915 607-611 VBD denotes were
T36914 612-621 VBN denotes performed
T36916 622-624 IN denotes in
T36917 625-635 NN denotes triplicate
T36918 636-638 IN denotes in
T36919 639-641 CD denotes 96
T36921 641-642 HYPH denotes -
T36920 642-646 NN denotes well
T36922 647-653 NN denotes format
T36923 654-659 VBG denotes using
T36924 660-666 NNP denotes Taqman
T36926 667-671 NN denotes core
T36925 672-680 NNS denotes reagents
T36927 681-684 CC denotes and
T36928 685-686 DT denotes a
T36930 687-692 NN denotes Prism
T36931 693-697 CD denotes 7500
T36932 698-706 NN denotes Sequence
T36929 707-715 NN denotes Detector
T36933 716-717 -LRB- denotes (
T36935 717-724 NNP denotes Applied
T36934 725-735 NNP denotes Biosystems
T36936 735-736 -RRB- denotes )
T36937 736-737 . denotes .
T36938 737-840 sentence denotes The mouse-specific primer-probe sets used to detect specific gene expression can be found in Table S1.
T36939 738-741 DT denotes The
T36941 742-747 NN denotes mouse
T36943 747-748 HYPH denotes -
T36942 748-756 JJ denotes specific
T36944 757-763 NN denotes primer
T36946 763-764 HYPH denotes -
T36945 764-769 NN denotes probe
T36940 770-774 NNS denotes sets
T36948 775-779 VBN denotes used
T36949 780-782 TO denotes to
T36950 783-789 VB denotes detect
T36951 790-798 JJ denotes specific
T36953 799-803 NN denotes gene
T36952 804-814 NN denotes expression
T36954 815-818 MD denotes can
T36955 819-821 VB denotes be
T36947 822-827 VBN denotes found
T36956 828-830 IN denotes in
T36957 831-836 NN denotes Table
T36958 837-839 NN denotes S1
T36959 839-840 . denotes .
T36960 840-899 sentence denotes The primers for UCP-1 have been previously described [42].
T36961 841-844 DT denotes The
T36962 845-852 NNS denotes primers
T36964 853-856 IN denotes for
T36965 857-860 NN denotes UCP
T36966 860-861 HYPH denotes -
T36967 861-862 CD denotes 1
T36968 863-867 VBP denotes have
T36969 868-872 VBN denotes been
T36970 873-883 RB denotes previously
T36963 884-893 VBN denotes described
T36971 894-895 -LRB- denotes [
T36972 895-897 CD denotes 42
T36973 897-898 -RRB- denotes ]
T36974 898-899 . denotes .
T36975 899-1046 sentence denotes Actin primer-probe set (Applied Biosystems) was included in a separate well and used to normalize the soleus, BAT, and heart gene expression data.
T36976 900-905 NN denotes Actin
T36978 906-912 NN denotes primer
T36980 912-913 HYPH denotes -
T36979 913-918 NN denotes probe
T36977 919-922 NN denotes set
T36982 923-924 -LRB- denotes (
T36984 924-931 NNP denotes Applied
T36983 932-942 NNP denotes Biosystems
T36985 942-943 -RRB- denotes )
T36986 944-947 VBD denotes was
T36981 948-956 VBN denotes included
T36987 957-959 IN denotes in
T36988 960-961 DT denotes a
T36990 962-970 JJ denotes separate
T36989 971-975 NN denotes well
T36991 976-979 CC denotes and
T36992 980-984 VBN denotes used
T36993 985-987 TO denotes to
T36994 988-997 VB denotes normalize
T36995 998-1001 DT denotes the
T36997 1002-1008 NN denotes soleus
T36998 1008-1010 , denotes ,
T36999 1010-1013 NN denotes BAT
T37000 1013-1015 , denotes ,
T37001 1015-1018 CC denotes and
T37002 1019-1024 NN denotes heart
T37003 1025-1029 NN denotes gene
T37004 1030-1040 NN denotes expression
T36996 1041-1045 NNS denotes data
T37005 1045-1046 . denotes .
T37006 1046-1160 sentence denotes GAPDH Rodent primers (Applied Biosystems) were used in the same well to normalize the liver gene expression data.
T37007 1047-1052 NN denotes GAPDH
T37009 1053-1059 NN denotes Rodent
T37008 1060-1067 NNS denotes primers
T37011 1068-1069 -LRB- denotes (
T37013 1069-1076 NNP denotes Applied
T37012 1077-1087 NNP denotes Biosystems
T37014 1087-1088 -RRB- denotes )
T37015 1089-1093 VBD denotes were
T37010 1094-1098 VBN denotes used
T37016 1099-1101 IN denotes in
T37017 1102-1105 DT denotes the
T37019 1106-1110 JJ denotes same
T37018 1111-1115 NN denotes well
T37020 1116-1118 TO denotes to
T37021 1119-1128 VB denotes normalize
T37022 1129-1132 DT denotes the
T37024 1133-1138 NN denotes liver
T37025 1139-1143 NN denotes gene
T37026 1144-1154 NN denotes expression
T37023 1155-1159 NNS denotes data
T37027 1159-1160 . denotes .
T37028 1160-1387 sentence denotes For Southern blot studies, 5 μg of genomic DNA was digested with Pst1 or Xba1, electrophoresed on a 0.8% TAE gel and transferred to a Gene Screen (Perkin Elmer, Wellesley, California, United States) membrane for hybridization.
T37029 1161-1164 IN denotes For
T37031 1165-1173 NNP denotes Southern
T37032 1174-1178 NN denotes blot
T37033 1179-1186 NNS denotes studies
T37034 1186-1188 , denotes ,
T37035 1188-1189 CD denotes 5
T37036 1190-1192 NN denotes μg
T37037 1193-1195 IN denotes of
T37038 1196-1203 JJ denotes genomic
T37039 1204-1207 NN denotes DNA
T37040 1208-1211 VBD denotes was
T37030 1212-1220 VBN denotes digested
T37041 1221-1225 IN denotes with
T37042 1226-1230 NN denotes Pst1
T37043 1231-1233 CC denotes or
T37044 1234-1238 NN denotes Xba1
T37045 1238-1240 , denotes ,
T37046 1240-1255 VBN denotes electrophoresed
T37047 1256-1258 IN denotes on
T37048 1259-1260 DT denotes a
T37050 1261-1264 CD denotes 0.8
T37051 1264-1265 NN denotes %
T37052 1266-1269 NN denotes TAE
T37049 1270-1273 NN denotes gel
T37053 1274-1277 CC denotes and
T37054 1278-1289 VBN denotes transferred
T37055 1290-1292 IN denotes to
T37056 1293-1294 DT denotes a
T37058 1295-1299 NN denotes Gene
T37059 1300-1306 NN denotes Screen
T37060 1307-1308 -LRB- denotes (
T37062 1308-1314 NNP denotes Perkin
T37061 1315-1320 NNP denotes Elmer
T37063 1320-1322 , denotes ,
T37064 1322-1331 NNP denotes Wellesley
T37065 1331-1333 , denotes ,
T37066 1333-1343 NNP denotes California
T37067 1343-1345 , denotes ,
T37068 1345-1351 NNP denotes United
T37069 1352-1358 NNP denotes States
T37070 1358-1359 -RRB- denotes )
T37057 1360-1368 NN denotes membrane
T37071 1369-1372 IN denotes for
T37072 1373-1386 NN denotes hybridization
T37073 1386-1387 . denotes .
T37074 1387-1559 sentence denotes Western blotting was performed as described [43] using the Enhanced Chemiluminescence detection system (Amersham Pharmacia Biotech, Piscataway, New Jersey, United States).
T37075 1388-1395 NNP denotes Western
T37076 1396-1404 NN denotes blotting
T37078 1405-1408 VBD denotes was
T37077 1409-1418 VBN denotes performed
T37079 1419-1421 IN denotes as
T37080 1422-1431 VBN denotes described
T37081 1432-1433 -LRB- denotes [
T37082 1433-1435 CD denotes 43
T37083 1435-1436 -RRB- denotes ]
T37084 1437-1442 VBG denotes using
T37085 1443-1446 DT denotes the
T37087 1447-1455 VBN denotes Enhanced
T37088 1456-1473 NN denotes Chemiluminescence
T37089 1474-1483 NN denotes detection
T37086 1484-1490 NN denotes system
T37090 1491-1492 -LRB- denotes (
T37092 1492-1500 NNP denotes Amersham
T37093 1501-1510 NNP denotes Pharmacia
T37091 1511-1518 NNP denotes Biotech
T37094 1518-1520 , denotes ,
T37095 1520-1530 NNP denotes Piscataway
T37096 1530-1532 , denotes ,
T37097 1532-1535 NNP denotes New
T37098 1536-1542 NNP denotes Jersey
T37099 1542-1544 , denotes ,
T37100 1544-1550 NNP denotes United
T37101 1551-1557 NNP denotes States
T37102 1557-1558 -RRB- denotes )
T37103 1558-1559 . denotes .
T37104 1559-1661 sentence denotes Ponceau S (Sigma, St. Louis, Missouri, United States) staining of the membrane was used as a control.
T37105 1560-1567 NNP denotes Ponceau
T37106 1568-1569 NNP denotes S
T37108 1570-1571 -LRB- denotes (
T37109 1571-1576 NNP denotes Sigma
T37110 1576-1578 , denotes ,
T37111 1578-1581 NNP denotes St.
T37112 1582-1587 NNP denotes Louis
T37113 1587-1589 , denotes ,
T37114 1589-1597 NNP denotes Missouri
T37115 1597-1599 , denotes ,
T37116 1599-1605 NNP denotes United
T37117 1606-1612 NNP denotes States
T37118 1612-1613 -RRB- denotes )
T37107 1614-1622 NN denotes staining
T37120 1623-1625 IN denotes of
T37121 1626-1629 DT denotes the
T37122 1630-1638 NN denotes membrane
T37123 1639-1642 VBD denotes was
T37119 1643-1647 VBN denotes used
T37124 1648-1650 IN denotes as
T37125 1651-1652 DT denotes a
T37126 1653-1660 NN denotes control
T37127 1660-1661 . denotes .
R9348 T36774 T36775 nmod RNA,analyses
R9349 T36776 T36774 punct ", ",RNA
R9350 T36777 T36774 conj DNA,RNA
R9351 T36778 T36777 punct ", ",DNA
R9352 T36779 T36777 cc and,DNA
R9353 T36780 T36777 conj protein,DNA
R9354 T36782 T36783 amod Total,RNA
R9355 T36783 T36784 nsubjpass RNA,isolated
R9356 T36785 T36784 auxpass was,isolated
R9357 T36786 T36784 prep by,isolated
R9358 T36787 T36788 det the,method
R9359 T36788 T36786 pobj method,by
R9360 T36789 T36788 compound RNAzol,method
R9361 T36790 T36791 punct (,Test
R9362 T36791 T36788 parataxis Test,method
R9363 T36792 T36791 compound Tel,Test
R9364 T36793 T36791 punct -,Test
R9365 T36794 T36791 punct ", ",Test
R9366 T36795 T36791 npadvmod Friendswood,Test
R9367 T36796 T36791 punct ", ",Test
R9368 T36797 T36791 npadvmod Texas,Test
R9369 T36798 T36791 punct ),Test
R9370 T36799 T36784 cc and,isolated
R9371 T36800 T36801 compound Northern,blotting
R9372 T36801 T36802 nsubjpass blotting,performed
R9373 T36802 T36784 conj performed,isolated
R9374 T36803 T36802 auxpass was,performed
R9375 T36804 T36805 mark as,described
R9376 T36805 T36802 advcl described,performed
R9377 T36806 T36805 advmod previously,described
R9378 T36807 T36808 punct [,40
R9379 T36808 T36805 parataxis 40,described
R9380 T36809 T36808 punct ],40
R9381 T36810 T36802 punct .,performed
R9382 T36812 T36813 det The,cDNAs
R9383 T36813 T36819 nsubj cDNAs,were
R9384 T36814 T36815 nmod PGC,1β
R9385 T36815 T36813 nmod 1β,cDNAs
R9386 T36816 T36815 punct -,1β
R9387 T36817 T36815 cc and,1β
R9388 T36818 T36815 conj PRC,1β
R9389 T36820 T36821 amod generous,gifts
R9390 T36821 T36819 attr gifts,were
R9391 T36822 T36821 prep from,gifts
R9392 T36823 T36824 compound Bruce,Spiegelman
R9393 T36824 T36822 pobj Spiegelman,from
R9394 T36825 T36824 cc and,Spiegelman
R9395 T36826 T36827 compound Richard,Scarpulla
R9396 T36827 T36824 conj Scarpulla,Spiegelman
R9397 T36828 T36819 punct ", ",were
R9398 T36829 T36819 advmod respectively,were
R9399 T36830 T36819 punct .,were
R9400 T36832 T36833 det The,cDNA
R9401 T36833 T36837 nsubj cDNA,was
R9402 T36834 T36833 nmod UCP,cDNA
R9403 T36835 T36834 punct -,UCP
R9404 T36836 T36834 nummod 1,UCP
R9405 T36838 T36839 det a,gift
R9406 T36839 T36837 attr gift,was
R9407 T36840 T36839 prep from,gift
R9408 T36841 T36842 compound Daniel,Ricquier
R9409 T36842 T36840 pobj Ricquier,from
R9410 T36843 T36837 punct .,was
R9411 T36845 T36846 compound RT,PCR
R9412 T36846 T36848 nsubjpass PCR,performed
R9413 T36847 T36846 punct -,PCR
R9414 T36849 T36848 auxpass was,performed
R9415 T36850 T36851 mark as,described
R9416 T36851 T36848 advcl described,performed
R9417 T36852 T36853 punct [,41
R9418 T36853 T36851 parataxis 41,described
R9419 T36854 T36853 punct ],41
R9420 T36855 T36848 punct .,performed
R9421 T36857 T36858 prep In,transcribed
R9422 T36859 T36857 amod brief,In
R9423 T36860 T36858 punct ", ",transcribed
R9424 T36861 T36862 amod total,RNA
R9425 T36862 T36858 nsubjpass RNA,transcribed
R9426 T36863 T36862 acl isolated,RNA
R9427 T36864 T36863 prep from,isolated
R9428 T36865 T36866 compound soleus,muscle
R9429 T36866 T36864 pobj muscle,from
R9430 T36867 T36866 punct ", ",muscle
R9431 T36868 T36866 conj BAT,muscle
R9432 T36869 T36868 punct ", ",BAT
R9433 T36870 T36868 cc and,BAT
R9434 T36871 T36868 conj heart,BAT
R9435 T36872 T36866 prep of,muscle
R9436 T36873 T36874 quantmod 1,2
R9437 T36874 T36876 nummod 2,mo
R9438 T36875 T36874 punct –,2
R9439 T36876 T36878 npadvmod mo,old
R9440 T36877 T36876 punct -,mo
R9441 T36878 T36880 amod old,mice
R9442 T36879 T36878 punct -,old
R9443 T36880 T36872 pobj mice,of
R9444 T36881 T36882 nmod PGC,1α
R9445 T36882 T36880 nmod 1α,mice
R9446 T36883 T36882 punct -,1α
R9447 T36884 T36882 punct +,1α
R9448 T36885 T36882 punct /,1α
R9449 T36886 T36882 punct +,1α
R9450 T36887 T36882 cc or,1α
R9451 T36888 T36882 conj PGCα,1α
R9452 T36889 T36888 punct −,PGCα
R9453 T36890 T36888 punct /,PGCα
R9454 T36891 T36888 punct −,PGCα
R9455 T36892 T36858 auxpass was,transcribed
R9456 T36893 T36858 advmod reverse,transcribed
R9457 T36894 T36858 prep with,transcribed
R9458 T36895 T36896 nmod Taqman,reagents
R9459 T36896 T36894 pobj reagents,with
R9460 T36897 T36898 amod reverse,transcription
R9461 T36898 T36896 compound transcription,reagents
R9462 T36899 T36900 punct (,Biosystems
R9463 T36900 T36896 parataxis Biosystems,reagents
R9464 T36901 T36900 compound Applied,Biosystems
R9465 T36902 T36900 punct ", ",Biosystems
R9466 T36903 T36904 compound Foster,City
R9467 T36904 T36900 npadvmod City,Biosystems
R9468 T36905 T36900 punct ", ",Biosystems
R9469 T36906 T36900 npadvmod California,Biosystems
R9470 T36907 T36900 punct ", ",Biosystems
R9471 T36908 T36909 compound United,States
R9472 T36909 T36900 npadvmod States,Biosystems
R9473 T36910 T36900 punct ),Biosystems
R9474 T36911 T36858 punct .,transcribed
R9475 T36913 T36914 nsubjpass Reactions,performed
R9476 T36915 T36914 auxpass were,performed
R9477 T36916 T36914 prep in,performed
R9478 T36917 T36916 pobj triplicate,in
R9479 T36918 T36914 prep in,performed
R9480 T36919 T36920 nummod 96,well
R9481 T36920 T36922 compound well,format
R9482 T36921 T36920 punct -,well
R9483 T36922 T36918 pobj format,in
R9484 T36923 T36914 advcl using,performed
R9485 T36924 T36925 compound Taqman,reagents
R9486 T36925 T36923 dobj reagents,using
R9487 T36926 T36925 compound core,reagents
R9488 T36927 T36925 cc and,reagents
R9489 T36928 T36929 det a,Detector
R9490 T36929 T36925 conj Detector,reagents
R9491 T36930 T36929 nmod Prism,Detector
R9492 T36931 T36930 nummod 7500,Prism
R9493 T36932 T36929 compound Sequence,Detector
R9494 T36933 T36934 punct (,Biosystems
R9495 T36934 T36929 parataxis Biosystems,Detector
R9496 T36935 T36934 compound Applied,Biosystems
R9497 T36936 T36934 punct ),Biosystems
R9498 T36937 T36914 punct .,performed
R9499 T36939 T36940 det The,sets
R9500 T36940 T36947 nsubjpass sets,found
R9501 T36941 T36942 npadvmod mouse,specific
R9502 T36942 T36940 amod specific,sets
R9503 T36943 T36942 punct -,specific
R9504 T36944 T36945 compound primer,probe
R9505 T36945 T36940 compound probe,sets
R9506 T36946 T36945 punct -,probe
R9507 T36948 T36940 acl used,sets
R9508 T36949 T36950 aux to,detect
R9509 T36950 T36948 advcl detect,used
R9510 T36951 T36952 amod specific,expression
R9511 T36952 T36950 dobj expression,detect
R9512 T36953 T36952 compound gene,expression
R9513 T36954 T36947 aux can,found
R9514 T36955 T36947 auxpass be,found
R9515 T36956 T36947 prep in,found
R9516 T36957 T36958 compound Table,S1
R9517 T36958 T36956 pobj S1,in
R9518 T36959 T36947 punct .,found
R9519 T36961 T36962 det The,primers
R9520 T36962 T36963 nsubjpass primers,described
R9521 T36964 T36962 prep for,primers
R9522 T36965 T36964 pobj UCP,for
R9523 T36966 T36965 punct -,UCP
R9524 T36967 T36965 nummod 1,UCP
R9525 T36968 T36963 aux have,described
R9526 T36969 T36963 auxpass been,described
R9527 T36970 T36963 advmod previously,described
R9528 T36971 T36972 punct [,42
R9529 T36972 T36963 parataxis 42,described
R9530 T36973 T36972 punct ],42
R9531 T36974 T36963 punct .,described
R9532 T36976 T36977 compound Actin,set
R9533 T36977 T36981 nsubjpass set,included
R9534 T36978 T36979 compound primer,probe
R9535 T36979 T36977 compound probe,set
R9536 T36980 T36979 punct -,probe
R9537 T36982 T36983 punct (,Biosystems
R9538 T36983 T36977 parataxis Biosystems,set
R9539 T36984 T36983 compound Applied,Biosystems
R9540 T36985 T36983 punct ),Biosystems
R9541 T36986 T36981 auxpass was,included
R9542 T36987 T36981 prep in,included
R9543 T36988 T36989 det a,well
R9544 T36989 T36987 pobj well,in
R9545 T36990 T36989 amod separate,well
R9546 T36991 T36981 cc and,included
R9547 T36992 T36981 conj used,included
R9548 T36993 T36994 aux to,normalize
R9549 T36994 T36992 advcl normalize,used
R9550 T36995 T36996 det the,data
R9551 T36996 T36994 dobj data,normalize
R9552 T36997 T36996 nmod soleus,data
R9553 T36998 T36997 punct ", ",soleus
R9554 T36999 T36997 conj BAT,soleus
R9555 T37000 T36999 punct ", ",BAT
R9556 T37001 T36999 cc and,BAT
R9557 T37002 T37003 compound heart,gene
R9558 T37003 T36999 conj gene,BAT
R9559 T37004 T36996 compound expression,data
R9560 T37005 T36981 punct .,included
R9561 T37007 T37008 compound GAPDH,primers
R9562 T37008 T37010 nsubjpass primers,used
R9563 T37009 T37008 compound Rodent,primers
R9564 T37011 T37012 punct (,Biosystems
R9565 T37012 T37008 parataxis Biosystems,primers
R9566 T37013 T37012 compound Applied,Biosystems
R9567 T37014 T37012 punct ),Biosystems
R9568 T37015 T37010 auxpass were,used
R9569 T37016 T37010 prep in,used
R9570 T37017 T37018 det the,well
R9571 T37018 T37016 pobj well,in
R9572 T37019 T37018 amod same,well
R9573 T37020 T37021 aux to,normalize
R9574 T37021 T37010 advcl normalize,used
R9575 T37022 T37023 det the,data
R9576 T37023 T37021 dobj data,normalize
R9577 T37024 T37025 compound liver,gene
R9578 T37025 T37023 compound gene,data
R9579 T37026 T37023 compound expression,data
R9580 T37027 T37010 punct .,used
R9581 T37029 T37030 prep For,digested
R9582 T37031 T37032 compound Southern,blot
R9583 T37032 T37033 compound blot,studies
R9584 T37033 T37029 pobj studies,For
R9585 T37034 T37030 punct ", ",digested
R9586 T37035 T37036 nummod 5,μg
R9587 T37036 T37030 nsubjpass μg,digested
R9588 T37037 T37036 prep of,μg
R9589 T37038 T37039 amod genomic,DNA
R9590 T37039 T37037 pobj DNA,of
R9591 T37040 T37030 auxpass was,digested
R9592 T37041 T37030 prep with,digested
R9593 T37042 T37041 pobj Pst1,with
R9594 T37043 T37042 cc or,Pst1
R9595 T37044 T37042 conj Xba1,Pst1
R9596 T37045 T37030 punct ", ",digested
R9597 T37046 T37030 conj electrophoresed,digested
R9598 T37047 T37046 prep on,electrophoresed
R9599 T37048 T37049 det a,gel
R9600 T37049 T37047 pobj gel,on
R9601 T37050 T37051 nummod 0.8,%
R9602 T37051 T37052 compound %,TAE
R9603 T37052 T37049 compound TAE,gel
R9604 T37053 T37046 cc and,electrophoresed
R9605 T37054 T37046 conj transferred,electrophoresed
R9606 T37055 T37054 prep to,transferred
R9607 T37056 T37057 det a,membrane
R9608 T37057 T37055 pobj membrane,to
R9609 T37058 T37059 nmod Gene,Screen
R9610 T37059 T37057 nmod Screen,membrane
R9611 T37060 T37061 punct (,Elmer
R9612 T37061 T37059 parataxis Elmer,Screen
R9613 T37062 T37061 compound Perkin,Elmer
R9614 T37063 T37061 punct ", ",Elmer
R9615 T37064 T37061 npadvmod Wellesley,Elmer
R9616 T37065 T37061 punct ", ",Elmer
R9617 T37066 T37061 npadvmod California,Elmer
R9618 T37067 T37061 punct ", ",Elmer
R9619 T37068 T37069 compound United,States
R9620 T37069 T37061 npadvmod States,Elmer
R9621 T37070 T37061 punct ),Elmer
R9622 T37071 T37054 prep for,transferred
R9623 T37072 T37071 pobj hybridization,for
R9624 T37073 T37030 punct .,digested
R9625 T37075 T37076 compound Western,blotting
R9626 T37076 T37077 nsubjpass blotting,performed
R9627 T37078 T37077 auxpass was,performed
R9628 T37079 T37080 mark as,described
R9629 T37080 T37077 advcl described,performed
R9630 T37081 T37082 punct [,43
R9631 T37082 T37080 parataxis 43,described
R9632 T37083 T37082 punct ],43
R9633 T37084 T37077 advcl using,performed
R9634 T37085 T37086 det the,system
R9635 T37086 T37084 dobj system,using
R9636 T37087 T37088 amod Enhanced,Chemiluminescence
R9637 T37088 T37086 compound Chemiluminescence,system
R9638 T37089 T37086 compound detection,system
R9639 T37090 T37091 punct (,Biotech
R9640 T37091 T37086 parataxis Biotech,system
R9641 T37092 T37091 compound Amersham,Biotech
R9642 T37093 T37091 compound Pharmacia,Biotech
R9643 T37094 T37091 punct ", ",Biotech
R9644 T37095 T37091 npadvmod Piscataway,Biotech
R9645 T37096 T37091 punct ", ",Biotech
R9646 T37097 T37098 compound New,Jersey
R9647 T37098 T37091 npadvmod Jersey,Biotech
R9648 T37099 T37091 punct ", ",Biotech
R9649 T37100 T37101 compound United,States
R9650 T37101 T37091 npadvmod States,Biotech
R9651 T37102 T37091 punct ),Biotech
R9652 T37103 T37077 punct .,performed
R9653 T37105 T37106 nmod Ponceau,S
R9654 T37106 T37107 nmod S,staining
R9655 T37107 T37119 nsubjpass staining,used
R9656 T37108 T37109 punct (,Sigma
R9657 T37109 T37106 parataxis Sigma,S
R9658 T37110 T37109 punct ", ",Sigma
R9659 T37111 T37112 compound St.,Louis
R9660 T37112 T37109 npadvmod Louis,Sigma
R9661 T37113 T37109 punct ", ",Sigma
R9662 T37114 T37109 npadvmod Missouri,Sigma
R9663 T37115 T37109 punct ", ",Sigma
R9664 T37116 T37117 compound United,States
R9665 T37117 T37109 npadvmod States,Sigma
R9666 T37118 T37109 punct ),Sigma
R9667 T37120 T37107 prep of,staining
R9668 T37121 T37122 det the,membrane
R9669 T37122 T37120 pobj membrane,of
R9670 T37123 T37119 auxpass was,used
R9671 T37124 T37119 prep as,used
R9672 T37125 T37126 det a,control
R9673 T37126 T37124 pobj control,as
R9674 T37127 T37119 punct .,used