craft-sa-dev Home  

PMC:1064854 / 57552-75833 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T34068 23-32 VBG denotes Targeting
T34069 33-36 DT denotes the
T34071 37-40 NN denotes PGC
T34073 40-41 HYPH denotes -
T34072 41-43 NN denotes 1α
T34070 44-48 NN denotes gene
T34074 49-51 IN denotes in
T34075 52-56 NNS denotes mice
T34076 56-225 sentence denotes A BAC genomic clone containing the murine PGC-1α gene, isolated from an Sv129 genomic library, was obtained from Incyte Genomics (Palo Alto, California, United States).
T34077 57-58 DT denotes A
T34079 59-62 NN denotes BAC
T34080 63-70 JJ denotes genomic
T34078 71-76 NN denotes clone
T34082 77-87 VBG denotes containing
T34083 88-91 DT denotes the
T34085 92-98 JJ denotes murine
T34086 99-102 NN denotes PGC
T34088 102-103 HYPH denotes -
T34087 103-105 NN denotes 1α
T34084 106-110 NN denotes gene
T34089 110-112 , denotes ,
T34090 112-120 VBN denotes isolated
T34091 121-125 IN denotes from
T34092 126-128 DT denotes an
T34094 129-134 NN denotes Sv129
T34095 135-142 JJ denotes genomic
T34093 143-150 NN denotes library
T34096 150-152 , denotes ,
T34097 152-155 VBD denotes was
T34081 156-164 VBN denotes obtained
T34098 165-169 IN denotes from
T34099 170-176 NNP denotes Incyte
T34100 177-185 NNP denotes Genomics
T34101 186-187 -LRB- denotes (
T34103 187-191 NNP denotes Palo
T34102 192-196 NNP denotes Alto
T34104 196-198 , denotes ,
T34105 198-208 NNP denotes California
T34106 208-210 , denotes ,
T34107 210-216 NNP denotes United
T34108 217-223 NNP denotes States
T34109 223-224 -RRB- denotes )
T34110 224-225 . denotes .
T34111 225-293 sentence denotes A 3-kb region spanning exon 3 was amplified from the genomic clone.
T34112 226-227 DT denotes A
T34114 228-229 CD denotes 3
T34116 229-230 HYPH denotes -
T34115 230-232 NN denotes kb
T34113 233-239 NN denotes region
T34118 240-248 VBG denotes spanning
T34119 249-253 NN denotes exon
T34120 254-255 CD denotes 3
T34121 256-259 VBD denotes was
T34117 260-269 VBN denotes amplified
T34122 270-274 IN denotes from
T34123 275-278 DT denotes the
T34125 279-286 JJ denotes genomic
T34124 287-292 NN denotes clone
T34126 292-293 . denotes .
T34127 293-477 sentence denotes A 5′ primer was designed to amplify a fragment with the 5′ end beginning 732 bp upstream of exon 3 just upstream of an endogenous Kpn1 restriction site (5′- AGTTTCCTTAGCAACTTCATA-3′).
T34128 294-295 DT denotes A
T34130 296-297 CD denotes 5
T34131 297-298 SYM denotes ′
T34129 299-305 NN denotes primer
T34133 306-309 VBD denotes was
T34132 310-318 VBN denotes designed
T34134 319-321 TO denotes to
T34135 322-329 VB denotes amplify
T34136 330-331 DT denotes a
T34137 332-340 NN denotes fragment
T34138 341-345 IN denotes with
T34139 346-349 DT denotes the
T34141 350-351 CD denotes 5
T34142 351-352 SYM denotes ′
T34140 353-356 NN denotes end
T34143 357-366 VBG denotes beginning
T34144 367-370 CD denotes 732
T34145 371-373 NN denotes bp
T34146 374-382 RB denotes upstream
T34147 383-385 IN denotes of
T34148 386-390 NN denotes exon
T34149 391-392 CD denotes 3
T34150 393-397 RB denotes just
T34151 398-406 RB denotes upstream
T34152 407-409 IN denotes of
T34153 410-412 DT denotes an
T34155 413-423 JJ denotes endogenous
T34156 424-428 NN denotes Kpn1
T34157 429-440 NN denotes restriction
T34154 441-445 NN denotes site
T34158 446-447 -LRB- denotes (
T34160 447-448 CD denotes 5
T34161 448-449 SYM denotes ′
T34162 449-450 HYPH denotes -
T34159 451-472 NN denotes AGTTTCCTTAGCAACTTCATA
T34163 472-473 HYPH denotes -
T34164 473-474 CD denotes 3
T34165 474-475 SYM denotes ′
T34166 475-476 -RRB- denotes )
T34167 476-477 . denotes .
T34168 477-600 sentence denotes The 3′ primer contained a BamH1 site engineered by mutating the bases shown in lowercase (5′- AAGGATTTTAgGATcc CAGTAC-3′).
T34169 478-481 DT denotes The
T34171 482-483 CD denotes 3
T34172 483-484 SYM denotes ′
T34170 485-491 NN denotes primer
T34173 492-501 VBD denotes contained
T34174 502-503 DT denotes a
T34176 504-509 NN denotes BamH1
T34175 510-514 NN denotes site
T34177 515-525 VBN denotes engineered
T34178 526-528 IN denotes by
T34179 529-537 VBG denotes mutating
T34180 538-541 DT denotes the
T34181 542-547 NNS denotes bases
T34182 548-553 VBN denotes shown
T34183 554-556 IN denotes in
T34184 557-566 NN denotes lowercase
T34185 567-568 -LRB- denotes (
T34187 568-569 CD denotes 5
T34188 569-570 SYM denotes ′
T34189 570-571 HYPH denotes -
T34186 572-595 NN denotes AAGGATTTTAgGATcc CAGTAC
T34190 595-596 HYPH denotes -
T34191 596-597 CD denotes 3
T34192 597-598 SYM denotes ′
T34193 598-599 -RRB- denotes )
T34194 599-600 . denotes .
T34195 600-654 sentence denotes A second fragment downstream of exon 5 was amplified.
T34196 601-602 DT denotes A
T34198 603-609 JJ denotes second
T34197 610-618 NN denotes fragment
T34200 619-629 RB denotes downstream
T34201 630-632 IN denotes of
T34202 633-637 NN denotes exon
T34203 638-639 CD denotes 5
T34204 640-643 VBD denotes was
T34199 644-653 VBN denotes amplified
T34205 653-654 . denotes .
T34206 654-823 sentence denotes In this latter amplicon, Not1 and Xho1 sites were engineered into the 5′ and 3′ primers, respectively (5′- TGGAGTgc GGCCGCTGGGA-3′ and 5′- AAAGAGTCTCgAg AATAGTTTCT-3′).
T34207 655-657 IN denotes In
T34209 658-662 DT denotes this
T34211 663-669 JJ denotes latter
T34210 670-678 NN denotes amplicon
T34212 678-680 , denotes ,
T34213 680-684 NN denotes Not1
T34215 685-688 CC denotes and
T34216 689-693 NN denotes Xho1
T34214 694-699 NNS denotes sites
T34217 700-704 VBD denotes were
T34208 705-715 VBN denotes engineered
T34218 716-720 IN denotes into
T34219 721-724 DT denotes the
T34220 725-726 CD denotes 5
T34222 726-727 SYM denotes ′
T34223 728-731 CC denotes and
T34224 732-733 CD denotes 3
T34225 733-734 SYM denotes ′
T34221 735-742 NNS denotes primers
T34226 742-744 , denotes ,
T34227 744-756 RB denotes respectively
T34228 757-758 -LRB- denotes (
T34230 758-759 CD denotes 5
T34231 759-760 SYM denotes ′
T34232 760-761 HYPH denotes -
T34229 762-782 NN denotes TGGAGTgc GGCCGCTGGGA
T34233 782-783 HYPH denotes -
T34234 783-784 CD denotes 3
T34235 784-785 SYM denotes ′
T34236 786-789 CC denotes and
T34237 790-791 CD denotes 5
T34239 791-792 SYM denotes ′
T34240 792-793 HYPH denotes -
T34238 794-818 NN denotes AAAGAGTCTCgAg AATAGTTTCT
T34241 818-819 HYPH denotes -
T34242 819-820 CD denotes 3
T34243 820-821 SYM denotes ′
T34244 821-822 -RRB- denotes )
T34245 822-823 . denotes .
T34246 823-891 sentence denotes The fragments were cloned into p1339-PGK-Neomycin targeting vector.
T34247 824-827 DT denotes The
T34248 828-837 NNS denotes fragments
T34250 838-842 VBD denotes were
T34249 843-849 VBN denotes cloned
T34251 850-854 IN denotes into
T34252 855-860 NN denotes p1339
T34254 860-861 HYPH denotes -
T34255 861-864 NN denotes PGK
T34256 864-865 HYPH denotes -
T34253 865-873 NN denotes Neomycin
T34258 874-883 NN denotes targeting
T34257 884-890 NN denotes vector
T34259 890-891 . denotes .
T34260 891-1005 sentence denotes The construct was linearized with Xho1 and electroporated into RW4 ES cells (Sv129 derived) using G418 selection.
T34261 892-895 DT denotes The
T34262 896-905 NN denotes construct
T34264 906-909 VBD denotes was
T34263 910-920 VBN denotes linearized
T34265 921-925 IN denotes with
T34266 926-930 NN denotes Xho1
T34267 931-934 CC denotes and
T34268 935-949 VBN denotes electroporated
T34269 950-954 IN denotes into
T34270 955-958 NN denotes RW4
T34272 959-961 NN denotes ES
T34271 962-967 NNS denotes cells
T34273 968-969 -LRB- denotes (
T34274 969-974 NN denotes Sv129
T34275 975-982 VBN denotes derived
T34276 982-983 -RRB- denotes )
T34277 984-989 VBG denotes using
T34278 990-994 NN denotes G418
T34279 995-1004 NN denotes selection
T34280 1004-1005 . denotes .
T34281 1005-1144 sentence denotes The electroporation was performed by the Siteman Cancer Center ES Cell Core at Washington University (St. Louis, Missouri, United States).
T34282 1006-1009 DT denotes The
T34283 1010-1025 NN denotes electroporation
T34285 1026-1029 VBD denotes was
T34284 1030-1039 VBN denotes performed
T34286 1040-1042 IN denotes by
T34287 1043-1046 DT denotes the
T34289 1047-1054 NNP denotes Siteman
T34291 1055-1061 NNP denotes Cancer
T34290 1062-1068 NNP denotes Center
T34292 1069-1071 NNP denotes ES
T34293 1072-1076 NNP denotes Cell
T34288 1077-1081 NNP denotes Core
T34294 1082-1084 IN denotes at
T34295 1085-1095 NNP denotes Washington
T34296 1096-1106 NNP denotes University
T34297 1107-1108 -LRB- denotes (
T34299 1108-1111 NNP denotes St.
T34298 1112-1117 NNP denotes Louis
T34300 1117-1119 , denotes ,
T34301 1119-1127 NNP denotes Missouri
T34302 1127-1129 , denotes ,
T34303 1129-1135 NNP denotes United
T34304 1136-1142 NNP denotes States
T34305 1142-1143 -RRB- denotes )
T34306 1143-1144 . denotes .
T34307 1144-1231 sentence denotes The clones were screened by Southern blot using an Xba1 digest (see Figure 1A and 1B).
T34308 1145-1148 DT denotes The
T34309 1149-1155 NNS denotes clones
T34311 1156-1160 VBD denotes were
T34310 1161-1169 VBN denotes screened
T34312 1170-1172 IN denotes by
T34313 1173-1181 NNP denotes Southern
T34314 1182-1186 NN denotes blot
T34315 1187-1192 VBG denotes using
T34316 1193-1195 DT denotes an
T34318 1196-1200 NN denotes Xba1
T34317 1201-1207 NN denotes digest
T34319 1208-1209 -LRB- denotes (
T34320 1209-1212 VB denotes see
T34321 1213-1219 NN denotes Figure
T34322 1220-1222 NN denotes 1A
T34323 1223-1226 CC denotes and
T34324 1227-1229 NN denotes 1B
T34325 1229-1230 -RRB- denotes )
T34326 1230-1231 . denotes .
T34327 1231-1367 sentence denotes One clone out of approximately 400 screened was positive for the homologous recombination on the 3′ end and an insertion on the 5′ end.
T34328 1232-1235 CD denotes One
T34329 1236-1241 NN denotes clone
T34331 1242-1245 IN denotes out
T34332 1246-1248 IN denotes of
T34333 1249-1262 RB denotes approximately
T34334 1263-1266 CD denotes 400
T34335 1267-1275 VBN denotes screened
T34330 1276-1279 VBD denotes was
T34336 1280-1288 JJ denotes positive
T34337 1289-1292 IN denotes for
T34338 1293-1296 DT denotes the
T34340 1297-1307 JJ denotes homologous
T34339 1308-1321 NN denotes recombination
T34341 1322-1324 IN denotes on
T34342 1325-1328 DT denotes the
T34344 1329-1330 CD denotes 3
T34345 1330-1331 SYM denotes ′
T34343 1332-1335 NN denotes end
T34346 1336-1339 CC denotes and
T34347 1340-1342 DT denotes an
T34348 1343-1352 NN denotes insertion
T34349 1353-1355 IN denotes on
T34350 1356-1359 DT denotes the
T34352 1360-1361 CD denotes 5
T34353 1361-1362 SYM denotes ′
T34351 1363-1366 NN denotes end
T34354 1366-1367 . denotes .
T34355 1367-1419 sentence denotes This clone was injected into a C57BL6/J blastocyst.
T34356 1368-1372 DT denotes This
T34357 1373-1378 NN denotes clone
T34359 1379-1382 VBD denotes was
T34358 1383-1391 VBN denotes injected
T34360 1392-1396 IN denotes into
T34361 1397-1398 DT denotes a
T34363 1399-1405 NN denotes C57BL6
T34365 1405-1406 HYPH denotes /
T34364 1406-1407 NN denotes J
T34362 1408-1418 NN denotes blastocyst
T34366 1418-1419 . denotes .
T34367 1419-1546 sentence denotes Chimeras were mated to C57BL6/J mice and germline transmission was confirmed by Southern blotting of tail DNA (see Figure 1B).
T34368 1420-1428 NNS denotes Chimeras
T34370 1429-1433 VBD denotes were
T34369 1434-1439 VBN denotes mated
T34371 1440-1442 IN denotes to
T34372 1443-1449 NN denotes C57BL6
T34374 1449-1450 HYPH denotes /
T34373 1450-1451 NN denotes J
T34375 1452-1456 NNS denotes mice
T34376 1457-1460 CC denotes and
T34377 1461-1469 NN denotes germline
T34378 1470-1482 NN denotes transmission
T34380 1483-1486 VBD denotes was
T34379 1487-1496 VBN denotes confirmed
T34381 1497-1499 IN denotes by
T34382 1500-1508 NNP denotes Southern
T34383 1509-1517 NN denotes blotting
T34384 1518-1520 IN denotes of
T34385 1521-1525 NN denotes tail
T34386 1526-1529 NN denotes DNA
T34387 1530-1531 -LRB- denotes (
T34388 1531-1534 VB denotes see
T34389 1535-1541 NN denotes Figure
T34390 1542-1544 NN denotes 1B
T34391 1544-1545 -RRB- denotes )
T34392 1545-1546 . denotes .
T34393 1546-1637 sentence denotes All experiments were performed using sex- and age-matched or littermate controls as noted.
T34394 1547-1550 DT denotes All
T34395 1551-1562 NNS denotes experiments
T34397 1563-1567 VBD denotes were
T34396 1568-1577 VBN denotes performed
T34398 1578-1583 VBG denotes using
T34399 1584-1587 NN denotes sex
T34401 1587-1588 HYPH denotes -
T34402 1589-1592 CC denotes and
T34403 1593-1596 NN denotes age
T34404 1596-1597 HYPH denotes -
T34400 1597-1604 VBN denotes matched
T34406 1605-1607 CC denotes or
T34407 1608-1618 NN denotes littermate
T34405 1619-1627 NNS denotes controls
T34408 1628-1630 IN denotes as
T34409 1631-1636 VBN denotes noted
T34410 1636-1637 . denotes .
T35414 1639-1646 JJ denotes General
T35416 1647-1653 NN denotes animal
T35415 1654-1661 NNS denotes studies
T35417 1661-1913 sentence denotes All animal experiments and euthanasia protocols were conducted in strict accordance with the National Institutes of Health guidelines for humane treatment of animals and were reviewed and approved by the Animal Care Committee of Washington University.
T35418 1662-1665 DT denotes All
T35420 1666-1672 NN denotes animal
T35419 1673-1684 NNS denotes experiments
T35422 1685-1688 CC denotes and
T35423 1689-1699 NN denotes euthanasia
T35424 1700-1709 NNS denotes protocols
T35425 1710-1714 VBD denotes were
T35421 1715-1724 VBN denotes conducted
T35426 1725-1727 IN denotes in
T35427 1728-1734 JJ denotes strict
T35428 1735-1745 NN denotes accordance
T35429 1746-1750 IN denotes with
T35430 1751-1754 DT denotes the
T35432 1755-1763 NNP denotes National
T35431 1764-1774 NNPS denotes Institutes
T35434 1775-1777 IN denotes of
T35435 1778-1784 NNP denotes Health
T35433 1785-1795 NNS denotes guidelines
T35436 1796-1799 IN denotes for
T35437 1800-1806 NN denotes humane
T35438 1807-1816 NN denotes treatment
T35439 1817-1819 IN denotes of
T35440 1820-1827 NNS denotes animals
T35441 1828-1831 CC denotes and
T35442 1832-1836 VBD denotes were
T35443 1837-1845 VBN denotes reviewed
T35444 1846-1849 CC denotes and
T35445 1850-1858 VBN denotes approved
T35446 1859-1861 IN denotes by
T35447 1862-1865 DT denotes the
T35449 1866-1872 NNP denotes Animal
T35450 1873-1877 NNP denotes Care
T35448 1878-1887 NNP denotes Committee
T35451 1888-1890 IN denotes of
T35452 1891-1901 NNP denotes Washington
T35453 1902-1912 NNP denotes University
T35454 1912-1913 . denotes .
T35455 1913-1960 sentence denotes Animals were weighed at different time points.
T35456 1914-1921 NNS denotes Animals
T35458 1922-1926 VBD denotes were
T35457 1927-1934 VBN denotes weighed
T35459 1935-1937 IN denotes at
T35460 1938-1947 JJ denotes different
T35462 1948-1952 NN denotes time
T35461 1953-1959 NNS denotes points
T35463 1959-1960 . denotes .
T35464 1960-2102 sentence denotes Male and female 3- to 8-wk-old PGC-1α+/+ and PGC-1α−/− mice were euthanized, and tissues were dissected and weighed on an analytical balance.
T35465 1961-1965 JJ denotes Male
T35467 1966-1969 CC denotes and
T35468 1970-1976 JJ denotes female
T35469 1977-1978 CD denotes 3
T35471 1978-1979 HYPH denotes -
T35472 1980-1982 IN denotes to
T35470 1983-1984 CD denotes 8
T35474 1984-1985 HYPH denotes -
T35473 1985-1987 NN denotes wk
T35476 1987-1988 HYPH denotes -
T35475 1988-1991 JJ denotes old
T35477 1992-1995 NN denotes PGC
T35479 1995-1996 HYPH denotes -
T35478 1996-1998 NN denotes 1α
T35480 1998-1999 SYM denotes +
T35481 1999-2000 HYPH denotes /
T35482 2000-2001 SYM denotes +
T35483 2002-2005 CC denotes and
T35484 2006-2009 NN denotes PGC
T35486 2009-2010 HYPH denotes -
T35485 2010-2012 NN denotes 1α
T35487 2012-2013 SYM denotes −
T35488 2013-2014 HYPH denotes /
T35489 2014-2015 SYM denotes −
T35466 2016-2020 NNS denotes mice
T35491 2021-2025 VBD denotes were
T35490 2026-2036 VBN denotes euthanized
T35492 2036-2038 , denotes ,
T35493 2038-2041 CC denotes and
T35494 2042-2049 NNS denotes tissues
T35496 2050-2054 VBD denotes were
T35495 2055-2064 VBN denotes dissected
T35497 2065-2068 CC denotes and
T35498 2069-2076 VBN denotes weighed
T35499 2077-2079 IN denotes on
T35500 2080-2082 DT denotes an
T35502 2083-2093 JJ denotes analytical
T35501 2094-2101 NN denotes balance
T35503 2101-2102 . denotes .
T35504 2102-2173 sentence denotes Tissue weights were corrected for total body weight before comparison.
T35505 2103-2109 NN denotes Tissue
T35506 2110-2117 NNS denotes weights
T35508 2118-2122 VBD denotes were
T35507 2123-2132 VBN denotes corrected
T35509 2133-2136 IN denotes for
T35510 2137-2142 JJ denotes total
T35512 2143-2147 NN denotes body
T35511 2148-2154 NN denotes weight
T35513 2155-2161 IN denotes before
T35514 2162-2172 NN denotes comparison
T35515 2172-2173 . denotes .
T35516 2173-2334 sentence denotes DEXA studies were performed as previously described [39] using a Lunar PIXIMUS DEXA system at 10, 18, and 24 wk in male and female PGC-1α+/+ and PGC-1α−/− mice.
T35517 2174-2178 NN denotes DEXA
T35518 2179-2186 NNS denotes studies
T35520 2187-2191 VBD denotes were
T35519 2192-2201 VBN denotes performed
T35521 2202-2204 IN denotes as
T35523 2205-2215 RB denotes previously
T35522 2216-2225 VBN denotes described
T35524 2226-2227 -LRB- denotes [
T35525 2227-2229 CD denotes 39
T35526 2229-2230 -RRB- denotes ]
T35527 2231-2236 VBG denotes using
T35528 2237-2238 DT denotes a
T35530 2239-2244 NNP denotes Lunar
T35531 2245-2252 NNP denotes PIXIMUS
T35532 2253-2257 NNP denotes DEXA
T35529 2258-2264 NN denotes system
T35533 2265-2267 IN denotes at
T35534 2268-2270 CD denotes 10
T35536 2270-2272 , denotes ,
T35537 2272-2274 CD denotes 18
T35538 2274-2276 , denotes ,
T35539 2276-2279 CC denotes and
T35540 2280-2282 CD denotes 24
T35535 2283-2285 NN denotes wk
T35541 2286-2288 IN denotes in
T35542 2289-2293 JJ denotes male
T35544 2294-2297 CC denotes and
T35545 2298-2304 JJ denotes female
T35546 2305-2308 NN denotes PGC
T35548 2308-2309 HYPH denotes -
T35547 2309-2311 NN denotes 1α
T35549 2311-2312 SYM denotes +
T35550 2312-2313 HYPH denotes /
T35551 2313-2314 SYM denotes +
T35552 2315-2318 CC denotes and
T35553 2319-2322 NN denotes PGC
T35555 2322-2323 HYPH denotes -
T35554 2323-2325 NN denotes 1α
T35556 2325-2326 SYM denotes −
T35557 2326-2327 HYPH denotes /
T35558 2327-2328 SYM denotes −
T35543 2329-2333 NNS denotes mice
T35559 2333-2334 . denotes .
T35560 2334-2470 sentence denotes For cold exposure experiments, male and female PGC-1α+/+ and PGC-1α−/− mice were singly housed and placed at 4 °C for 5 h without food.
T35561 2335-2338 IN denotes For
T35563 2339-2343 NN denotes cold
T35565 2344-2352 NN denotes exposure
T35564 2353-2364 NNS denotes experiments
T35566 2364-2366 , denotes ,
T35567 2366-2370 JJ denotes male
T35569 2371-2374 CC denotes and
T35570 2375-2381 JJ denotes female
T35571 2382-2385 NN denotes PGC
T35573 2385-2386 HYPH denotes -
T35572 2386-2388 NN denotes 1α
T35574 2388-2389 SYM denotes +
T35575 2389-2390 HYPH denotes /
T35576 2390-2391 SYM denotes +
T35577 2392-2395 CC denotes and
T35578 2396-2399 NN denotes PGC
T35580 2399-2400 HYPH denotes -
T35579 2400-2402 NN denotes 1α
T35581 2402-2403 SYM denotes −
T35582 2403-2404 HYPH denotes /
T35583 2404-2405 SYM denotes −
T35568 2406-2410 NNS denotes mice
T35584 2411-2415 VBD denotes were
T35585 2416-2422 RB denotes singly
T35562 2423-2429 VBN denotes housed
T35586 2430-2433 CC denotes and
T35587 2434-2440 VBN denotes placed
T35588 2441-2443 IN denotes at
T35589 2444-2445 CD denotes 4
T35590 2446-2448 NN denotes °C
T35591 2449-2452 IN denotes for
T35592 2453-2454 CD denotes 5
T35593 2455-2456 NN denotes h
T35594 2457-2464 IN denotes without
T35595 2465-2469 NN denotes food
T35596 2469-2470 . denotes .
T35597 2470-2563 sentence denotes Core body temperatures were monitored by rectal probe at baseline and every hour thereafter.
T35598 2471-2475 NN denotes Core
T35600 2476-2480 NN denotes body
T35599 2481-2493 NNS denotes temperatures
T35602 2494-2498 VBD denotes were
T35601 2499-2508 VBN denotes monitored
T35603 2509-2511 IN denotes by
T35604 2512-2518 JJ denotes rectal
T35605 2519-2524 NN denotes probe
T35606 2525-2527 IN denotes at
T35607 2528-2536 NN denotes baseline
T35608 2537-2540 CC denotes and
T35609 2541-2546 DT denotes every
T35610 2547-2551 NN denotes hour
T35611 2552-2562 RB denotes thereafter
T35612 2562-2563 . denotes .
T35613 2563-2628 sentence denotes Mice were monitored at least every 30 min to check for lethargy.
T35614 2564-2568 NNS denotes Mice
T35616 2569-2573 VBD denotes were
T35615 2574-2583 VBN denotes monitored
T35617 2584-2586 RB denotes at
T35618 2587-2592 RBS denotes least
T35620 2593-2598 DT denotes every
T35621 2599-2601 CD denotes 30
T35619 2602-2605 NN denotes min
T35622 2606-2608 TO denotes to
T35623 2609-2614 VB denotes check
T35624 2615-2618 IN denotes for
T35625 2619-2627 NN denotes lethargy
T35626 2627-2628 . denotes .
T35627 2628-2722 sentence denotes At the end of 5 h, mice were sacrificed and tissues harvested for RNA and protein extraction.
T35628 2629-2631 IN denotes At
T35630 2632-2635 DT denotes the
T35631 2636-2639 NN denotes end
T35632 2640-2642 IN denotes of
T35633 2643-2644 CD denotes 5
T35634 2645-2646 NN denotes h
T35635 2646-2648 , denotes ,
T35636 2648-2652 NNS denotes mice
T35637 2653-2657 VBD denotes were
T35629 2658-2668 VBN denotes sacrificed
T35638 2669-2672 CC denotes and
T35639 2673-2680 NNS denotes tissues
T35640 2681-2690 VBN denotes harvested
T35641 2691-2694 IN denotes for
T35642 2695-2698 NN denotes RNA
T35644 2699-2702 CC denotes and
T35645 2703-2710 NN denotes protein
T35643 2711-2721 NN denotes extraction
T35646 2721-2722 . denotes .
T35647 2722-2798 sentence denotes For fasting studies, animals were singly housed and given water ad libitum.
T35648 2723-2726 IN denotes For
T35650 2727-2734 NN denotes fasting
T35651 2735-2742 NNS denotes studies
T35652 2742-2744 , denotes ,
T35653 2744-2751 NNS denotes animals
T35654 2752-2756 VBD denotes were
T35655 2757-2763 RB denotes singly
T35649 2764-2770 VBN denotes housed
T35656 2771-2774 CC denotes and
T35657 2775-2780 VBN denotes given
T35658 2781-2786 NN denotes water
T35659 2787-2789 FW denotes ad
T35660 2790-2797 FW denotes libitum
T35661 2797-2798 . denotes .
T35662 2798-2894 sentence denotes Food was removed from cages in the morning and tissues harvested at 24 h for RNA and histology.
T35663 2799-2803 NN denotes Food
T35665 2804-2807 VBD denotes was
T35664 2808-2815 VBN denotes removed
T35666 2816-2820 IN denotes from
T35667 2821-2826 NNS denotes cages
T35668 2827-2829 IN denotes in
T35669 2830-2833 DT denotes the
T35670 2834-2841 NN denotes morning
T35671 2842-2845 CC denotes and
T35672 2846-2853 NNS denotes tissues
T35673 2854-2863 VBN denotes harvested
T35674 2864-2866 IN denotes at
T35675 2867-2869 CD denotes 24
T35676 2870-2871 NN denotes h
T35677 2872-2875 IN denotes for
T35678 2876-2879 NN denotes RNA
T35679 2880-2883 CC denotes and
T35680 2884-2893 NN denotes histology
T35681 2893-2894 . denotes .
T35682 2894-2987 sentence denotes Photography of the mice was performed by MedPic at Washington University School of Medicine.
T35683 2895-2906 NN denotes Photography
T35685 2907-2909 IN denotes of
T35686 2910-2913 DT denotes the
T35687 2914-2918 NNS denotes mice
T35688 2919-2922 VBD denotes was
T35684 2923-2932 VBN denotes performed
T35689 2933-2935 IN denotes by
T35690 2936-2942 NNP denotes MedPic
T35691 2943-2945 IN denotes at
T35692 2946-2956 NNP denotes Washington
T35693 2957-2967 NNP denotes University
T35694 2968-2974 NNP denotes School
T35695 2975-2977 IN denotes of
T35696 2978-2986 NNP denotes Medicine
T35697 2986-2987 . denotes .
T35698 2987-3231 sentence denotes 48-h activity monitoring was performed by JAX Services (The Jackson Laboratory, West Sacramento, California, United States) using a Comprehensive Laboratory Animal Monitoring System (CLAMS, Columbus Instruments, Columbus, Ohio, United States).
T35699 2988-2990 CD denotes 48
T35701 2990-2991 HYPH denotes -
T35700 2991-2992 NN denotes h
T35703 2993-3001 NN denotes activity
T35702 3002-3012 NN denotes monitoring
T35705 3013-3016 VBD denotes was
T35704 3017-3026 VBN denotes performed
T35706 3027-3029 IN denotes by
T35707 3030-3033 NNP denotes JAX
T35708 3034-3042 NNPS denotes Services
T35709 3043-3044 -LRB- denotes (
T35711 3044-3047 DT denotes The
T35712 3048-3055 NNP denotes Jackson
T35710 3056-3066 NNP denotes Laboratory
T35713 3066-3068 , denotes ,
T35714 3068-3072 NNP denotes West
T35715 3073-3083 NNP denotes Sacramento
T35716 3083-3085 , denotes ,
T35717 3085-3095 NNP denotes California
T35718 3095-3097 , denotes ,
T35719 3097-3103 NNP denotes United
T35720 3104-3110 NNP denotes States
T35721 3110-3111 -RRB- denotes )
T35722 3112-3117 VBG denotes using
T35723 3118-3119 DT denotes a
T35725 3120-3133 NNP denotes Comprehensive
T35726 3134-3144 NNP denotes Laboratory
T35727 3145-3151 NNP denotes Animal
T35728 3152-3162 NNP denotes Monitoring
T35724 3163-3169 NNP denotes System
T35729 3170-3171 -LRB- denotes (
T35730 3171-3176 NNP denotes CLAMS
T35731 3176-3178 , denotes ,
T35732 3178-3186 NNP denotes Columbus
T35733 3187-3198 NNP denotes Instruments
T35734 3198-3200 , denotes ,
T35735 3200-3208 NNP denotes Columbus
T35736 3208-3210 , denotes ,
T35737 3210-3214 NNP denotes Ohio
T35738 3214-3216 , denotes ,
T35739 3216-3222 NNP denotes United
T35740 3223-3229 NNP denotes States
T35741 3229-3230 -RRB- denotes )
T35742 3230-3231 . denotes .
T35743 3231-3310 sentence denotes Briefly, 3-mo-old female mice were acclimated for 17 h before data collection.
T35744 3232-3239 RB denotes Briefly
T35746 3239-3241 , denotes ,
T35747 3241-3242 CD denotes 3
T35749 3242-3243 HYPH denotes -
T35748 3243-3245 NN denotes mo
T35751 3245-3246 HYPH denotes -
T35750 3246-3249 JJ denotes old
T35753 3250-3256 JJ denotes female
T35752 3257-3261 NNS denotes mice
T35754 3262-3266 VBD denotes were
T35745 3267-3277 VBN denotes acclimated
T35755 3278-3281 IN denotes for
T35756 3282-3284 CD denotes 17
T35757 3285-3286 NN denotes h
T35758 3287-3293 IN denotes before
T35759 3294-3298 NNS denotes data
T35760 3299-3309 NN denotes collection
T35761 3309-3310 . denotes .
T35762 3310-3344 sentence denotes Data were collected every 30 min.
T35763 3311-3315 NNS denotes Data
T35765 3316-3320 VBD denotes were
T35764 3321-3330 VBN denotes collected
T35766 3331-3336 DT denotes every
T35768 3337-3339 CD denotes 30
T35767 3340-3343 NN denotes min
T35769 3343-3344 . denotes .
T35770 3344-3463 sentence denotes Total beam breaks in the XY direction were tabulated for the 12-h light and dark cycles and compared across genotypes.
T35771 3345-3350 JJ denotes Total
T35773 3351-3355 NN denotes beam
T35772 3356-3362 NNS denotes breaks
T35775 3363-3365 IN denotes in
T35776 3366-3369 DT denotes the
T35778 3370-3372 NN denotes XY
T35777 3373-3382 NN denotes direction
T35779 3383-3387 VBD denotes were
T35774 3388-3397 VBN denotes tabulated
T35780 3398-3401 IN denotes for
T35781 3402-3405 DT denotes the
T35783 3406-3408 CD denotes 12
T35785 3408-3409 HYPH denotes -
T35784 3409-3410 NN denotes h
T35786 3411-3416 NN denotes light
T35787 3417-3420 CC denotes and
T35788 3421-3425 NN denotes dark
T35782 3426-3432 NNS denotes cycles
T35789 3433-3436 CC denotes and
T35790 3437-3445 VBN denotes compared
T35791 3446-3452 IN denotes across
T35792 3453-3462 NNS denotes genotypes
T35793 3462-3463 . denotes .
T36774 3465-3468 NN denotes RNA
T36776 3468-3470 , denotes ,
T36777 3470-3473 NN denotes DNA
T36778 3473-3475 , denotes ,
T36779 3475-3478 CC denotes and
T36780 3479-3486 NN denotes protein
T36775 3487-3495 NNS denotes analyses
T36781 3495-3636 sentence denotes Total RNA was isolated by the RNAzol method (Tel-Test, Friendswood, Texas) and Northern blotting was performed as previously described [40].
T36782 3496-3501 JJ denotes Total
T36783 3502-3505 NN denotes RNA
T36785 3506-3509 VBD denotes was
T36784 3510-3518 VBN denotes isolated
T36786 3519-3521 IN denotes by
T36787 3522-3525 DT denotes the
T36789 3526-3532 NN denotes RNAzol
T36788 3533-3539 NN denotes method
T36790 3540-3541 -LRB- denotes (
T36792 3541-3544 NNP denotes Tel
T36793 3544-3545 HYPH denotes -
T36791 3545-3549 NNP denotes Test
T36794 3549-3551 , denotes ,
T36795 3551-3562 NNP denotes Friendswood
T36796 3562-3564 , denotes ,
T36797 3564-3569 NNP denotes Texas
T36798 3569-3570 -RRB- denotes )
T36799 3571-3574 CC denotes and
T36800 3575-3583 NNP denotes Northern
T36801 3584-3592 NN denotes blotting
T36803 3593-3596 VBD denotes was
T36802 3597-3606 VBN denotes performed
T36804 3607-3609 IN denotes as
T36806 3610-3620 RB denotes previously
T36805 3621-3630 VBN denotes described
T36807 3631-3632 -LRB- denotes [
T36808 3632-3634 CD denotes 40
T36809 3634-3635 -RRB- denotes ]
T36810 3635-3636 . denotes .
T36811 3636-3740 sentence denotes The PGC-1β and PRC cDNAs were generous gifts from Bruce Spiegelman and Richard Scarpulla, respectively.
T36812 3637-3640 DT denotes The
T36814 3641-3644 NN denotes PGC
T36816 3644-3645 HYPH denotes -
T36815 3645-3647 NN denotes 1β
T36817 3648-3651 CC denotes and
T36818 3652-3655 NN denotes PRC
T36813 3656-3661 NNS denotes cDNAs
T36819 3662-3666 VBD denotes were
T36820 3667-3675 JJ denotes generous
T36821 3676-3681 NNS denotes gifts
T36822 3682-3686 IN denotes from
T36823 3687-3692 NNP denotes Bruce
T36824 3693-3703 NNP denotes Spiegelman
T36825 3704-3707 CC denotes and
T36826 3708-3715 NNP denotes Richard
T36827 3716-3725 NNP denotes Scarpulla
T36828 3725-3727 , denotes ,
T36829 3727-3739 RB denotes respectively
T36830 3739-3740 . denotes .
T36831 3740-3788 sentence denotes The UCP-1 cDNA was a gift from Daniel Ricquier.
T36832 3741-3744 DT denotes The
T36834 3745-3748 NN denotes UCP
T36835 3748-3749 HYPH denotes -
T36836 3749-3750 CD denotes 1
T36833 3751-3755 NN denotes cDNA
T36837 3756-3759 VBD denotes was
T36838 3760-3761 DT denotes a
T36839 3762-3766 NN denotes gift
T36840 3767-3771 IN denotes from
T36841 3772-3778 NNP denotes Daniel
T36842 3779-3787 NNP denotes Ricquier
T36843 3787-3788 . denotes .
T36844 3788-3828 sentence denotes RT-PCR was performed as described [41].
T36845 3789-3791 NN denotes RT
T36847 3791-3792 HYPH denotes -
T36846 3792-3795 NN denotes PCR
T36849 3796-3799 VBD denotes was
T36848 3800-3809 VBN denotes performed
T36850 3810-3812 IN denotes as
T36851 3813-3822 VBN denotes described
T36852 3823-3824 -LRB- denotes [
T36853 3824-3826 CD denotes 41
T36854 3826-3827 -RRB- denotes ]
T36855 3827-3828 . denotes .
T36856 3828-4061 sentence denotes In brief, total RNA isolated from soleus muscle, BAT, and heart of 1–2-mo-old PGC-1α+/+ or PGCα−/− mice was reverse transcribed with Taqman reverse transcription reagents (Applied Biosystems, Foster City, California, United States).
T36857 3829-3831 IN denotes In
T36859 3832-3837 JJ denotes brief
T36860 3837-3839 , denotes ,
T36861 3839-3844 JJ denotes total
T36862 3845-3848 NN denotes RNA
T36863 3849-3857 VBN denotes isolated
T36864 3858-3862 IN denotes from
T36865 3863-3869 NN denotes soleus
T36866 3870-3876 NN denotes muscle
T36867 3876-3878 , denotes ,
T36868 3878-3881 NN denotes BAT
T36869 3881-3883 , denotes ,
T36870 3883-3886 CC denotes and
T36871 3887-3892 NN denotes heart
T36872 3893-3895 IN denotes of
T36873 3896-3897 CD denotes 1
T36875 3897-3898 SYM denotes –
T36874 3898-3899 CD denotes 2
T36877 3899-3900 HYPH denotes -
T36876 3900-3902 NN denotes mo
T36879 3902-3903 HYPH denotes -
T36878 3903-3906 JJ denotes old
T36881 3907-3910 NN denotes PGC
T36883 3910-3911 HYPH denotes -
T36882 3911-3913 NN denotes 1α
T36884 3913-3914 SYM denotes +
T36885 3914-3915 HYPH denotes /
T36886 3915-3916 SYM denotes +
T36887 3917-3919 CC denotes or
T36888 3920-3924 NN denotes PGCα
T36889 3924-3925 SYM denotes −
T36890 3925-3926 HYPH denotes /
T36891 3926-3927 SYM denotes −
T36880 3928-3932 NNS denotes mice
T36892 3933-3936 VBD denotes was
T36893 3937-3944 RB denotes reverse
T36858 3945-3956 VBN denotes transcribed
T36894 3957-3961 IN denotes with
T36895 3962-3968 NNP denotes Taqman
T36897 3969-3976 JJ denotes reverse
T36898 3977-3990 NN denotes transcription
T36896 3991-3999 NNS denotes reagents
T36899 4000-4001 -LRB- denotes (
T36901 4001-4008 NNP denotes Applied
T36900 4009-4019 NNP denotes Biosystems
T36902 4019-4021 , denotes ,
T36903 4021-4027 NNP denotes Foster
T36904 4028-4032 NNP denotes City
T36905 4032-4034 , denotes ,
T36906 4034-4044 NNP denotes California
T36907 4044-4046 , denotes ,
T36908 4046-4052 NNP denotes United
T36909 4053-4059 NNP denotes States
T36910 4059-4060 -RRB- denotes )
T36911 4060-4061 . denotes .
T36912 4061-4202 sentence denotes Reactions were performed in triplicate in 96-well format using Taqman core reagents and a Prism 7500 Sequence Detector (Applied Biosystems).
T36913 4062-4071 NNS denotes Reactions
T36915 4072-4076 VBD denotes were
T36914 4077-4086 VBN denotes performed
T36916 4087-4089 IN denotes in
T36917 4090-4100 NN denotes triplicate
T36918 4101-4103 IN denotes in
T36919 4104-4106 CD denotes 96
T36921 4106-4107 HYPH denotes -
T36920 4107-4111 NN denotes well
T36922 4112-4118 NN denotes format
T36923 4119-4124 VBG denotes using
T36924 4125-4131 NNP denotes Taqman
T36926 4132-4136 NN denotes core
T36925 4137-4145 NNS denotes reagents
T36927 4146-4149 CC denotes and
T36928 4150-4151 DT denotes a
T36930 4152-4157 NN denotes Prism
T36931 4158-4162 CD denotes 7500
T36932 4163-4171 NN denotes Sequence
T36929 4172-4180 NN denotes Detector
T36933 4181-4182 -LRB- denotes (
T36935 4182-4189 NNP denotes Applied
T36934 4190-4200 NNP denotes Biosystems
T36936 4200-4201 -RRB- denotes )
T36937 4201-4202 . denotes .
T36938 4202-4305 sentence denotes The mouse-specific primer-probe sets used to detect specific gene expression can be found in Table S1.
T36939 4203-4206 DT denotes The
T36941 4207-4212 NN denotes mouse
T36943 4212-4213 HYPH denotes -
T36942 4213-4221 JJ denotes specific
T36944 4222-4228 NN denotes primer
T36946 4228-4229 HYPH denotes -
T36945 4229-4234 NN denotes probe
T36940 4235-4239 NNS denotes sets
T36948 4240-4244 VBN denotes used
T36949 4245-4247 TO denotes to
T36950 4248-4254 VB denotes detect
T36951 4255-4263 JJ denotes specific
T36953 4264-4268 NN denotes gene
T36952 4269-4279 NN denotes expression
T36954 4280-4283 MD denotes can
T36955 4284-4286 VB denotes be
T36947 4287-4292 VBN denotes found
T36956 4293-4295 IN denotes in
T36957 4296-4301 NN denotes Table
T36958 4302-4304 NN denotes S1
T36959 4304-4305 . denotes .
T36960 4305-4364 sentence denotes The primers for UCP-1 have been previously described [42].
T36961 4306-4309 DT denotes The
T36962 4310-4317 NNS denotes primers
T36964 4318-4321 IN denotes for
T36965 4322-4325 NN denotes UCP
T36966 4325-4326 HYPH denotes -
T36967 4326-4327 CD denotes 1
T36968 4328-4332 VBP denotes have
T36969 4333-4337 VBN denotes been
T36970 4338-4348 RB denotes previously
T36963 4349-4358 VBN denotes described
T36971 4359-4360 -LRB- denotes [
T36972 4360-4362 CD denotes 42
T36973 4362-4363 -RRB- denotes ]
T36974 4363-4364 . denotes .
T36975 4364-4511 sentence denotes Actin primer-probe set (Applied Biosystems) was included in a separate well and used to normalize the soleus, BAT, and heart gene expression data.
T36976 4365-4370 NN denotes Actin
T36978 4371-4377 NN denotes primer
T36980 4377-4378 HYPH denotes -
T36979 4378-4383 NN denotes probe
T36977 4384-4387 NN denotes set
T36982 4388-4389 -LRB- denotes (
T36984 4389-4396 NNP denotes Applied
T36983 4397-4407 NNP denotes Biosystems
T36985 4407-4408 -RRB- denotes )
T36986 4409-4412 VBD denotes was
T36981 4413-4421 VBN denotes included
T36987 4422-4424 IN denotes in
T36988 4425-4426 DT denotes a
T36990 4427-4435 JJ denotes separate
T36989 4436-4440 NN denotes well
T36991 4441-4444 CC denotes and
T36992 4445-4449 VBN denotes used
T36993 4450-4452 TO denotes to
T36994 4453-4462 VB denotes normalize
T36995 4463-4466 DT denotes the
T36997 4467-4473 NN denotes soleus
T36998 4473-4475 , denotes ,
T36999 4475-4478 NN denotes BAT
T37000 4478-4480 , denotes ,
T37001 4480-4483 CC denotes and
T37002 4484-4489 NN denotes heart
T37003 4490-4494 NN denotes gene
T37004 4495-4505 NN denotes expression
T36996 4506-4510 NNS denotes data
T37005 4510-4511 . denotes .
T37006 4511-4625 sentence denotes GAPDH Rodent primers (Applied Biosystems) were used in the same well to normalize the liver gene expression data.
T37007 4512-4517 NN denotes GAPDH
T37009 4518-4524 NN denotes Rodent
T37008 4525-4532 NNS denotes primers
T37011 4533-4534 -LRB- denotes (
T37013 4534-4541 NNP denotes Applied
T37012 4542-4552 NNP denotes Biosystems
T37014 4552-4553 -RRB- denotes )
T37015 4554-4558 VBD denotes were
T37010 4559-4563 VBN denotes used
T37016 4564-4566 IN denotes in
T37017 4567-4570 DT denotes the
T37019 4571-4575 JJ denotes same
T37018 4576-4580 NN denotes well
T37020 4581-4583 TO denotes to
T37021 4584-4593 VB denotes normalize
T37022 4594-4597 DT denotes the
T37024 4598-4603 NN denotes liver
T37025 4604-4608 NN denotes gene
T37026 4609-4619 NN denotes expression
T37023 4620-4624 NNS denotes data
T37027 4624-4625 . denotes .
T37028 4625-4852 sentence denotes For Southern blot studies, 5 μg of genomic DNA was digested with Pst1 or Xba1, electrophoresed on a 0.8% TAE gel and transferred to a Gene Screen (Perkin Elmer, Wellesley, California, United States) membrane for hybridization.
T37029 4626-4629 IN denotes For
T37031 4630-4638 NNP denotes Southern
T37032 4639-4643 NN denotes blot
T37033 4644-4651 NNS denotes studies
T37034 4651-4653 , denotes ,
T37035 4653-4654 CD denotes 5
T37036 4655-4657 NN denotes μg
T37037 4658-4660 IN denotes of
T37038 4661-4668 JJ denotes genomic
T37039 4669-4672 NN denotes DNA
T37040 4673-4676 VBD denotes was
T37030 4677-4685 VBN denotes digested
T37041 4686-4690 IN denotes with
T37042 4691-4695 NN denotes Pst1
T37043 4696-4698 CC denotes or
T37044 4699-4703 NN denotes Xba1
T37045 4703-4705 , denotes ,
T37046 4705-4720 VBN denotes electrophoresed
T37047 4721-4723 IN denotes on
T37048 4724-4725 DT denotes a
T37050 4726-4729 CD denotes 0.8
T37051 4729-4730 NN denotes %
T37052 4731-4734 NN denotes TAE
T37049 4735-4738 NN denotes gel
T37053 4739-4742 CC denotes and
T37054 4743-4754 VBN denotes transferred
T37055 4755-4757 IN denotes to
T37056 4758-4759 DT denotes a
T37058 4760-4764 NN denotes Gene
T37059 4765-4771 NN denotes Screen
T37060 4772-4773 -LRB- denotes (
T37062 4773-4779 NNP denotes Perkin
T37061 4780-4785 NNP denotes Elmer
T37063 4785-4787 , denotes ,
T37064 4787-4796 NNP denotes Wellesley
T37065 4796-4798 , denotes ,
T37066 4798-4808 NNP denotes California
T37067 4808-4810 , denotes ,
T37068 4810-4816 NNP denotes United
T37069 4817-4823 NNP denotes States
T37070 4823-4824 -RRB- denotes )
T37057 4825-4833 NN denotes membrane
T37071 4834-4837 IN denotes for
T37072 4838-4851 NN denotes hybridization
T37073 4851-4852 . denotes .
T37074 4852-5024 sentence denotes Western blotting was performed as described [43] using the Enhanced Chemiluminescence detection system (Amersham Pharmacia Biotech, Piscataway, New Jersey, United States).
T37075 4853-4860 NNP denotes Western
T37076 4861-4869 NN denotes blotting
T37078 4870-4873 VBD denotes was
T37077 4874-4883 VBN denotes performed
T37079 4884-4886 IN denotes as
T37080 4887-4896 VBN denotes described
T37081 4897-4898 -LRB- denotes [
T37082 4898-4900 CD denotes 43
T37083 4900-4901 -RRB- denotes ]
T37084 4902-4907 VBG denotes using
T37085 4908-4911 DT denotes the
T37087 4912-4920 VBN denotes Enhanced
T37088 4921-4938 NN denotes Chemiluminescence
T37089 4939-4948 NN denotes detection
T37086 4949-4955 NN denotes system
T37090 4956-4957 -LRB- denotes (
T37092 4957-4965 NNP denotes Amersham
T37093 4966-4975 NNP denotes Pharmacia
T37091 4976-4983 NNP denotes Biotech
T37094 4983-4985 , denotes ,
T37095 4985-4995 NNP denotes Piscataway
T37096 4995-4997 , denotes ,
T37097 4997-5000 NNP denotes New
T37098 5001-5007 NNP denotes Jersey
T37099 5007-5009 , denotes ,
T37100 5009-5015 NNP denotes United
T37101 5016-5022 NNP denotes States
T37102 5022-5023 -RRB- denotes )
T37103 5023-5024 . denotes .
T37104 5024-5126 sentence denotes Ponceau S (Sigma, St. Louis, Missouri, United States) staining of the membrane was used as a control.
T37105 5025-5032 NNP denotes Ponceau
T37106 5033-5034 NNP denotes S
T37108 5035-5036 -LRB- denotes (
T37109 5036-5041 NNP denotes Sigma
T37110 5041-5043 , denotes ,
T37111 5043-5046 NNP denotes St.
T37112 5047-5052 NNP denotes Louis
T37113 5052-5054 , denotes ,
T37114 5054-5062 NNP denotes Missouri
T37115 5062-5064 , denotes ,
T37116 5064-5070 NNP denotes United
T37117 5071-5077 NNP denotes States
T37118 5077-5078 -RRB- denotes )
T37107 5079-5087 NN denotes staining
T37120 5088-5090 IN denotes of
T37121 5091-5094 DT denotes the
T37122 5095-5103 NN denotes membrane
T37123 5104-5107 VBD denotes was
T37119 5108-5112 VBN denotes used
T37124 5113-5115 IN denotes as
T37125 5116-5117 DT denotes a
T37126 5118-5125 NN denotes control
T37127 5125-5126 . denotes .
T38198 5128-5141 JJ denotes Mitochondrial
T38199 5142-5153 NN denotes respiration
T38200 5154-5161 NNS denotes studies
T38201 5161-5323 sentence denotes Mitochondrial respiration was assessed in saponin-skinned soleus fibers with succinate as substrate and in the presence of rotenone as previously described [44].
T38202 5162-5175 JJ denotes Mitochondrial
T38203 5176-5187 NN denotes respiration
T38205 5188-5191 VBD denotes was
T38204 5192-5200 VBN denotes assessed
T38206 5201-5203 IN denotes in
T38207 5204-5211 NN denotes saponin
T38209 5211-5212 HYPH denotes -
T38208 5212-5219 VBN denotes skinned
T38211 5220-5226 NN denotes soleus
T38210 5227-5233 NNS denotes fibers
T38212 5234-5238 IN denotes with
T38213 5239-5248 NN denotes succinate
T38214 5249-5251 IN denotes as
T38215 5252-5261 NN denotes substrate
T38216 5262-5265 CC denotes and
T38217 5266-5268 IN denotes in
T38218 5269-5272 DT denotes the
T38219 5273-5281 NN denotes presence
T38220 5282-5284 IN denotes of
T38221 5285-5293 NN denotes rotenone
T38222 5294-5296 IN denotes as
T38224 5297-5307 RB denotes previously
T38223 5308-5317 VBN denotes described
T38225 5318-5319 -LRB- denotes [
T38226 5319-5321 CD denotes 44
T38227 5321-5322 -RRB- denotes ]
T38228 5322-5323 . denotes .
T38229 5323-5421 sentence denotes In brief, 3-mo-old female mice were anesthetized with chloral hydrate (400 mg/kg of body weight).
T38230 5324-5326 IN denotes In
T38232 5327-5332 JJ denotes brief
T38233 5332-5334 , denotes ,
T38234 5334-5335 CD denotes 3
T38236 5335-5336 HYPH denotes -
T38235 5336-5338 NN denotes mo
T38238 5338-5339 HYPH denotes -
T38237 5339-5342 JJ denotes old
T38240 5343-5349 JJ denotes female
T38239 5350-5354 NNS denotes mice
T38241 5355-5359 VBD denotes were
T38231 5360-5372 VBN denotes anesthetized
T38242 5373-5377 IN denotes with
T38243 5378-5385 JJ denotes chloral
T38244 5386-5393 NN denotes hydrate
T38245 5394-5395 -LRB- denotes (
T38247 5395-5398 CD denotes 400
T38246 5399-5401 NN denotes mg
T38248 5401-5402 SYM denotes /
T38249 5402-5404 NN denotes kg
T38250 5405-5407 IN denotes of
T38251 5408-5412 NN denotes body
T38252 5413-5419 NN denotes weight
T38253 5419-5420 -RRB- denotes )
T38254 5420-5421 . denotes .
T38255 5421-5732 sentence denotes Soleus fibers were separated and then transferred to a buffer (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 6.56 mM MgCl2, 20 mM imidazole, 53.3 mM K-MES, 20 mM taurine, 5.3 mM ATP, 15 mM PCr, 3 mM KH2PO4, 0.5 mM DTT [pH 7.1]) supplemented with 50 μg/ml saponin and permeabilized for 30 min at 4 °C with gentle stirring.
T38256 5422-5428 NN denotes Soleus
T38257 5429-5435 NNS denotes fibers
T38259 5436-5440 VBD denotes were
T38258 5441-5450 VBN denotes separated
T38260 5451-5454 CC denotes and
T38261 5455-5459 RB denotes then
T38262 5460-5471 VBN denotes transferred
T38263 5472-5474 IN denotes to
T38264 5475-5476 DT denotes a
T38265 5477-5483 NN denotes buffer
T38266 5484-5485 -LRB- denotes (
T38267 5485-5489 CD denotes 2.77
T38268 5490-5492 NN denotes mM
T38270 5493-5497 NN denotes K2Ca
T38271 5497-5498 HYPH denotes -
T38269 5498-5502 NN denotes EGTA
T38272 5502-5504 , denotes ,
T38273 5504-5508 CD denotes 7.23
T38274 5509-5511 NN denotes mM
T38275 5512-5518 NN denotes K2EGTA
T38276 5518-5520 , denotes ,
T38277 5520-5524 CD denotes 6.56
T38278 5525-5527 NN denotes mM
T38279 5528-5533 NN denotes MgCl2
T38280 5533-5535 , denotes ,
T38281 5535-5537 CD denotes 20
T38282 5538-5540 NN denotes mM
T38283 5541-5550 NN denotes imidazole
T38284 5550-5552 , denotes ,
T38285 5552-5556 CD denotes 53.3
T38286 5557-5559 NN denotes mM
T38288 5560-5561 NN denotes K
T38289 5561-5562 HYPH denotes -
T38287 5562-5565 NN denotes MES
T38290 5565-5567 , denotes ,
T38291 5567-5569 CD denotes 20
T38292 5570-5572 NN denotes mM
T38293 5573-5580 NN denotes taurine
T38294 5580-5582 , denotes ,
T38295 5582-5585 CD denotes 5.3
T38296 5586-5588 NN denotes mM
T38297 5589-5592 NN denotes ATP
T38298 5592-5594 , denotes ,
T38299 5594-5596 CD denotes 15
T38300 5597-5599 NN denotes mM
T38301 5600-5603 NN denotes PCr
T38302 5603-5605 , denotes ,
T38303 5605-5606 CD denotes 3
T38304 5607-5609 NN denotes mM
T38305 5610-5616 NN denotes KH2PO4
T38306 5616-5618 , denotes ,
T38307 5618-5621 CD denotes 0.5
T38308 5622-5624 NN denotes mM
T38309 5625-5628 NN denotes DTT
T38310 5629-5630 -LRB- denotes [
T38311 5630-5632 NN denotes pH
T38312 5633-5636 CD denotes 7.1
T38313 5636-5637 -RRB- denotes ]
T38314 5637-5638 -RRB- denotes )
T38315 5639-5651 VBN denotes supplemented
T38316 5652-5656 IN denotes with
T38317 5657-5659 CD denotes 50
T38318 5660-5662 NN denotes μg
T38319 5662-5663 SYM denotes /
T38321 5663-5665 NN denotes ml
T38320 5666-5673 NN denotes saponin
T38322 5674-5677 CC denotes and
T38323 5678-5691 VBN denotes permeabilized
T38324 5692-5695 IN denotes for
T38325 5696-5698 CD denotes 30
T38326 5699-5702 NN denotes min
T38327 5703-5705 IN denotes at
T38328 5706-5707 CD denotes 4
T38329 5708-5710 NN denotes °C
T38330 5711-5715 IN denotes with
T38331 5716-5722 JJ denotes gentle
T38332 5723-5731 NN denotes stirring
T38333 5731-5732 . denotes .
T38334 5732-5923 sentence denotes Fibers were then washed twice for 10 min each (2.77 mM K2Ca-EGTA, 7.23 mM K2EGTA, 1.38 mM MgCl2, 20 mM imidazole, 100 mM K-MES, 20 mM taurine, 3 mM KH2PO4, 0.5 mM DTT, 2 mg/ml BSA [pH 7.1]).
T38335 5733-5739 NNS denotes Fibers
T38337 5740-5744 VBD denotes were
T38338 5745-5749 RB denotes then
T38336 5750-5756 VBN denotes washed
T38339 5757-5762 RB denotes twice
T38340 5763-5766 IN denotes for
T38341 5767-5769 CD denotes 10
T38342 5770-5773 NN denotes min
T38343 5774-5778 RB denotes each
T38344 5779-5780 -LRB- denotes (
T38346 5780-5784 CD denotes 2.77
T38347 5785-5787 NN denotes mM
T38348 5788-5792 NN denotes K2Ca
T38349 5792-5793 HYPH denotes -
T38345 5793-5797 NN denotes EGTA
T38350 5797-5799 , denotes ,
T38351 5799-5803 CD denotes 7.23
T38352 5804-5806 NN denotes mM
T38353 5807-5813 NN denotes K2EGTA
T38354 5813-5815 , denotes ,
T38355 5815-5819 CD denotes 1.38
T38356 5820-5822 NN denotes mM
T38357 5823-5828 NN denotes MgCl2
T38358 5828-5830 , denotes ,
T38359 5830-5832 CD denotes 20
T38360 5833-5835 NN denotes mM
T38361 5836-5845 NN denotes imidazole
T38362 5845-5847 , denotes ,
T38363 5847-5850 CD denotes 100
T38364 5851-5853 NN denotes mM
T38366 5854-5855 NN denotes K
T38367 5855-5856 HYPH denotes -
T38365 5856-5859 NN denotes MES
T38368 5859-5861 , denotes ,
T38369 5861-5863 CD denotes 20
T38370 5864-5866 NN denotes mM
T38371 5867-5874 NN denotes taurine
T38372 5874-5876 , denotes ,
T38373 5876-5877 CD denotes 3
T38374 5878-5880 NN denotes mM
T38375 5881-5887 NN denotes KH2PO4
T38376 5887-5889 , denotes ,
T38377 5889-5892 CD denotes 0.5
T38378 5893-5895 NN denotes mM
T38379 5896-5899 NN denotes DTT
T38380 5899-5901 , denotes ,
T38381 5901-5902 CD denotes 2
T38382 5903-5905 NN denotes mg
T38383 5905-5906 SYM denotes /
T38385 5906-5908 NN denotes ml
T38384 5909-5912 NN denotes BSA
T38386 5913-5914 -LRB- denotes [
T38387 5914-5916 NN denotes pH
T38388 5917-5920 CD denotes 7.1
T38389 5920-5921 -RRB- denotes ]
T38390 5921-5922 -RRB- denotes )
T38391 5922-5923 . denotes .
T38392 5923-6048 sentence denotes Respiration was measured at 25 °C using an optical probe (Oxygen FOXY Probe, Ocean Optics, Dunedin, Florida, United States).
T38393 5924-5935 NN denotes Respiration
T38395 5936-5939 VBD denotes was
T38394 5940-5948 VBN denotes measured
T38396 5949-5951 IN denotes at
T38397 5952-5954 CD denotes 25
T38398 5955-5957 NN denotes °C
T38399 5958-5963 VBG denotes using
T38400 5964-5966 DT denotes an
T38402 5967-5974 JJ denotes optical
T38401 5975-5980 NN denotes probe
T38403 5981-5982 -LRB- denotes (
T38405 5982-5988 NNP denotes Oxygen
T38406 5989-5993 NNP denotes FOXY
T38404 5994-5999 NNP denotes Probe
T38407 5999-6001 , denotes ,
T38408 6001-6006 NNP denotes Ocean
T38409 6007-6013 NNP denotes Optics
T38410 6013-6015 , denotes ,
T38411 6015-6022 NNP denotes Dunedin
T38412 6022-6024 , denotes ,
T38413 6024-6031 NNP denotes Florida
T38414 6031-6033 , denotes ,
T38415 6033-6039 NNP denotes United
T38416 6040-6046 NNP denotes States
T38417 6046-6047 -RRB- denotes )
T38418 6047-6048 . denotes .
T38419 6048-6192 sentence denotes Following measurement of basal state 2 respiration, maximal (ADP-stimulated) state 3 respiration was determined by exposing fibers to 1 mM ADP.
T38420 6049-6058 VBG denotes Following
T38422 6059-6070 NN denotes measurement
T38423 6071-6073 IN denotes of
T38424 6074-6079 JJ denotes basal
T38426 6080-6085 NN denotes state
T38427 6086-6087 CD denotes 2
T38425 6088-6099 NN denotes respiration
T38428 6099-6101 , denotes ,
T38429 6101-6108 JJ denotes maximal
T38431 6109-6110 -LRB- denotes (
T38432 6110-6113 NN denotes ADP
T38434 6113-6114 HYPH denotes -
T38433 6114-6124 VBN denotes stimulated
T38435 6124-6125 -RRB- denotes )
T38436 6126-6131 NN denotes state
T38437 6132-6133 CD denotes 3
T38430 6134-6145 NN denotes respiration
T38438 6146-6149 VBD denotes was
T38421 6150-6160 VBN denotes determined
T38439 6161-6163 IN denotes by
T38440 6164-6172 VBG denotes exposing
T38441 6173-6179 NNS denotes fibers
T38442 6180-6182 IN denotes to
T38443 6183-6184 CD denotes 1
T38444 6185-6187 NN denotes mM
T38445 6188-6191 NN denotes ADP
T38446 6191-6192 . denotes .
T38447 6192-6319 sentence denotes The integrity of the outer mitochondrial membrane was assessed by adding 8 μM exogenous cytochrome c to ADP-stimulated fibers.
T38448 6193-6196 DT denotes The
T38449 6197-6206 NN denotes integrity
T38451 6207-6209 IN denotes of
T38452 6210-6213 DT denotes the
T38454 6214-6219 JJ denotes outer
T38455 6220-6233 JJ denotes mitochondrial
T38453 6234-6242 NN denotes membrane
T38456 6243-6246 VBD denotes was
T38450 6247-6255 VBN denotes assessed
T38457 6256-6258 IN denotes by
T38458 6259-6265 VBG denotes adding
T38459 6266-6267 CD denotes 8
T38460 6268-6270 NN denotes μM
T38462 6271-6280 JJ denotes exogenous
T38463 6281-6291 NN denotes cytochrome
T38461 6292-6293 NN denotes c
T38464 6294-6296 IN denotes to
T38465 6297-6300 NN denotes ADP
T38467 6300-6301 HYPH denotes -
T38466 6301-6311 VBN denotes stimulated
T38468 6312-6318 NNS denotes fibers
T38469 6318-6319 . denotes .
T38470 6319-6409 sentence denotes State 4 respiration (uncoupled) was evaluated following addition of oligomycin (1 μg/ml).
T38471 6320-6325 NN denotes State
T38473 6326-6327 CD denotes 4
T38472 6328-6339 NN denotes respiration
T38475 6340-6341 -LRB- denotes (
T38476 6341-6350 JJ denotes uncoupled
T38477 6350-6351 -RRB- denotes )
T38478 6352-6355 VBD denotes was
T38474 6356-6365 VBN denotes evaluated
T38479 6366-6375 VBG denotes following
T38480 6376-6384 NN denotes addition
T38481 6385-6387 IN denotes of
T38482 6388-6398 NN denotes oligomycin
T38483 6399-6400 -LRB- denotes (
T38485 6400-6401 CD denotes 1
T38484 6402-6404 NN denotes μg
T38486 6404-6405 SYM denotes /
T38487 6405-6407 NN denotes ml
T38488 6407-6408 -RRB- denotes )
T38489 6408-6409 . denotes .
T38490 6409-6505 sentence denotes The solubility of oxygen in the respiration buffer at 25 °C was taken as 246.87 nmol O2 · ml−1.
T38491 6410-6413 DT denotes The
T38492 6414-6424 NN denotes solubility
T38494 6425-6427 IN denotes of
T38495 6428-6434 NN denotes oxygen
T38496 6435-6437 IN denotes in
T38497 6438-6441 DT denotes the
T38499 6442-6453 NN denotes respiration
T38498 6454-6460 NN denotes buffer
T38500 6461-6463 IN denotes at
T38501 6464-6466 CD denotes 25
T38502 6467-6469 NN denotes °C
T38503 6470-6473 VBD denotes was
T38493 6474-6479 VBN denotes taken
T38504 6480-6482 IN denotes as
T38505 6483-6489 CD denotes 246.87
T38506 6490-6494 NN denotes nmol
T38507 6495-6497 NN denotes O2
T38508 6498-6499 SYM denotes ·
T38509 6500-6502 NN denotes ml
T38510 6502-6503 SYM denotes −
T38511 6503-6504 CD denotes 1
T38512 6504-6505 . denotes .
T38513 6505-6567 sentence denotes Respiration rates were expressed as nmol O2 · min−1 · mgdw−1.
T38514 6506-6517 NN denotes Respiration
T38515 6518-6523 NNS denotes rates
T38517 6524-6528 VBD denotes were
T38516 6529-6538 VBN denotes expressed
T38518 6539-6541 IN denotes as
T38519 6542-6546 NN denotes nmol
T38520 6547-6549 NN denotes O2
T38521 6550-6551 SYM denotes ·
T38522 6552-6555 NN denotes min
T38523 6555-6556 SYM denotes −
T38524 6556-6557 CD denotes 1
T38525 6558-6559 SYM denotes ·
T38526 6560-6564 NN denotes mgdw
T38527 6564-6565 SYM denotes −
T38528 6565-6566 CD denotes 1
T38529 6566-6567 . denotes .
T38945 6569-6576 NN denotes Insulin
T38947 6577-6580 CC denotes and
T38948 6581-6588 NN denotes glucose
T38946 6589-6598 NN denotes tolerance
T38949 6599-6604 NNS denotes tests
T38950 6604-6674 sentence denotes Glucose and Insulin tolerance tests were performed as described [35].
T38951 6605-6612 NN denotes Glucose
T38953 6613-6616 CC denotes and
T38954 6617-6624 NN denotes Insulin
T38952 6625-6634 NN denotes tolerance
T38955 6635-6640 NNS denotes tests
T38957 6641-6645 VBD denotes were
T38956 6646-6655 VBN denotes performed
T38958 6656-6658 IN denotes as
T38959 6659-6668 VBN denotes described
T38960 6669-6670 -LRB- denotes [
T38961 6670-6672 CD denotes 35
T38962 6672-6673 -RRB- denotes ]
T38963 6673-6674 . denotes .
T38964 6674-6739 sentence denotes Prior to studies, mice were fasted overnight (GTT) or 6 h (ITT).
T38965 6675-6680 JJ denotes Prior
T38966 6681-6683 IN denotes to
T38968 6684-6691 NNS denotes studies
T38969 6691-6693 , denotes ,
T38970 6693-6697 NNS denotes mice
T38971 6698-6702 VBD denotes were
T38967 6703-6709 VBN denotes fasted
T38972 6710-6719 RB denotes overnight
T38974 6720-6721 -LRB- denotes (
T38975 6721-6724 NN denotes GTT
T38976 6724-6725 -RRB- denotes )
T38977 6726-6728 CC denotes or
T38978 6729-6730 CD denotes 6
T38973 6731-6732 NN denotes h
T38979 6733-6734 -LRB- denotes (
T38980 6734-6737 NN denotes ITT
T38981 6737-6738 -RRB- denotes )
T38982 6738-6739 . denotes .
T38983 6739-6817 sentence denotes In GTT studies, mice were injected with a 10% solution of D-glucose (1 g/kg).
T38984 6740-6742 IN denotes In
T38986 6743-6746 NN denotes GTT
T38987 6747-6754 NNS denotes studies
T38988 6754-6756 , denotes ,
T38989 6756-6760 NNS denotes mice
T38990 6761-6765 VBD denotes were
T38985 6766-6774 VBN denotes injected
T38991 6775-6779 IN denotes with
T38992 6780-6781 DT denotes a
T38994 6782-6784 CD denotes 10
T38995 6784-6785 NN denotes %
T38993 6786-6794 NN denotes solution
T38996 6795-6797 IN denotes of
T38997 6798-6799 NN denotes D
T38999 6799-6800 HYPH denotes -
T38998 6800-6807 NN denotes glucose
T39000 6808-6809 -LRB- denotes (
T39002 6809-6810 CD denotes 1
T39001 6811-6812 NN denotes g
T39003 6812-6813 SYM denotes /
T39004 6813-6815 NN denotes kg
T39005 6815-6816 -RRB- denotes )
T39006 6816-6817 . denotes .
T39007 6817-6974 sentence denotes For ITT, mice received an IP injection of human regular insulin (Eli Lilly, Indianapolis, Indiana, United States) at a dose of 0.75 units/kg of body weight.
T39008 6818-6821 IN denotes For
T39010 6822-6825 NN denotes ITT
T39011 6825-6827 , denotes ,
T39012 6827-6831 NNS denotes mice
T39009 6832-6840 VBD denotes received
T39013 6841-6843 DT denotes an
T39015 6844-6846 NN denotes IP
T39014 6847-6856 NN denotes injection
T39016 6857-6859 IN denotes of
T39017 6860-6865 JJ denotes human
T39019 6866-6873 JJ denotes regular
T39018 6874-6881 NN denotes insulin
T39020 6882-6883 -LRB- denotes (
T39022 6883-6886 NNP denotes Eli
T39021 6887-6892 NNP denotes Lilly
T39023 6892-6894 , denotes ,
T39024 6894-6906 NNP denotes Indianapolis
T39025 6906-6908 , denotes ,
T39026 6908-6915 NNP denotes Indiana
T39027 6915-6917 , denotes ,
T39028 6917-6923 NNP denotes United
T39029 6924-6930 NNP denotes States
T39030 6930-6931 -RRB- denotes )
T39031 6932-6934 IN denotes at
T39032 6935-6936 DT denotes a
T39033 6937-6941 NN denotes dose
T39034 6942-6944 IN denotes of
T39035 6945-6949 CD denotes 0.75
T39036 6950-6955 NNS denotes units
T39037 6955-6956 SYM denotes /
T39038 6956-6958 NN denotes kg
T39039 6959-6961 IN denotes of
T39040 6962-6966 NN denotes body
T39041 6967-6973 NN denotes weight
T39042 6973-6974 . denotes .
T39043 6974-7110 sentence denotes Tail blood glucose was determined at 0, 30, 60, and 120 min after challenge using a B-GLUCOSE Analyzer (Hemacue AB, Angelholm, Sweden).
T39044 6975-6979 NN denotes Tail
T39046 6980-6985 NN denotes blood
T39045 6986-6993 NN denotes glucose
T39048 6994-6997 VBD denotes was
T39047 6998-7008 VBN denotes determined
T39049 7009-7011 IN denotes at
T39050 7012-7013 CD denotes 0
T39052 7013-7015 , denotes ,
T39053 7015-7017 CD denotes 30
T39054 7017-7019 , denotes ,
T39055 7019-7021 CD denotes 60
T39056 7021-7023 , denotes ,
T39057 7023-7026 CC denotes and
T39058 7027-7030 CD denotes 120
T39051 7031-7034 NN denotes min
T39059 7035-7040 IN denotes after
T39060 7041-7050 NN denotes challenge
T39061 7051-7056 VBG denotes using
T39062 7057-7058 DT denotes a
T39064 7059-7060 NNP denotes B
T39066 7060-7061 HYPH denotes -
T39065 7061-7068 NNP denotes GLUCOSE
T39063 7069-7077 NNP denotes Analyzer
T39067 7078-7079 -LRB- denotes (
T39069 7079-7086 NNP denotes Hemacue
T39068 7087-7089 NNP denotes AB
T39070 7089-7091 , denotes ,
T39071 7091-7100 NNP denotes Angelholm
T39072 7100-7102 , denotes ,
T39073 7102-7108 NNP denotes Sweden
T39074 7108-7109 -RRB- denotes )
T39075 7109-7110 . denotes .
T39319 7112-7120 JJ denotes Indirect
T39320 7121-7132 NN denotes calorimetry
T39321 7132-7245 sentence denotes Oxygen consumption rates (VO2) of 5-wk-old female mice were measured using a Columbus Instruments Oxymax System.
T39322 7133-7139 NN denotes Oxygen
T39324 7140-7151 NN denotes consumption
T39323 7152-7157 NNS denotes rates
T39326 7158-7159 -LRB- denotes (
T39327 7159-7162 NN denotes VO2
T39328 7162-7163 -RRB- denotes )
T39329 7164-7166 IN denotes of
T39330 7167-7168 CD denotes 5
T39332 7168-7169 HYPH denotes -
T39331 7169-7171 NN denotes wk
T39334 7171-7172 HYPH denotes -
T39333 7172-7175 JJ denotes old
T39336 7176-7182 JJ denotes female
T39335 7183-7187 NNS denotes mice
T39337 7188-7192 VBD denotes were
T39325 7193-7201 VBN denotes measured
T39338 7202-7207 VBG denotes using
T39339 7208-7209 DT denotes a
T39341 7210-7218 NNP denotes Columbus
T39342 7219-7230 NNPS denotes Instruments
T39343 7231-7237 NNP denotes Oxymax
T39340 7238-7244 NNP denotes System
T39344 7244-7245 . denotes .
T39345 7245-7321 sentence denotes Resting baseline oxygen consumption rates were assessed for at least 1.0 h.
T39346 7246-7253 NN denotes Resting
T39348 7254-7262 NN denotes baseline
T39349 7263-7269 NN denotes oxygen
T39350 7270-7281 NN denotes consumption
T39347 7282-7287 NNS denotes rates
T39352 7288-7292 VBD denotes were
T39351 7293-7301 VBN denotes assessed
T39353 7302-7305 IN denotes for
T39354 7306-7308 RB denotes at
T39355 7309-7314 RBS denotes least
T39356 7315-7318 CD denotes 1.0
T39357 7319-7320 NN denotes h
T39358 7320-7321 . denotes .
T39359 7321-7456 sentence denotes For β3-adrenergic stimulation studies, BRL 37344 (Sigma) was dissolved in sterile saline and injected IP (2 μg/g of body weight) [45].
T39360 7322-7325 IN denotes For
T39362 7326-7328 NN denotes β3
T39364 7328-7329 HYPH denotes -
T39363 7329-7339 JJ denotes adrenergic
T39366 7340-7351 NN denotes stimulation
T39365 7352-7359 NNS denotes studies
T39367 7359-7361 , denotes ,
T39368 7361-7364 NN denotes BRL
T39369 7365-7370 CD denotes 37344
T39370 7371-7372 -LRB- denotes (
T39371 7372-7377 NNP denotes Sigma
T39372 7377-7378 -RRB- denotes )
T39373 7379-7382 VBD denotes was
T39361 7383-7392 VBN denotes dissolved
T39374 7393-7395 IN denotes in
T39375 7396-7403 JJ denotes sterile
T39376 7404-7410 NN denotes saline
T39377 7411-7414 CC denotes and
T39378 7415-7423 VBN denotes injected
T39379 7424-7426 RB denotes IP
T39380 7427-7428 -LRB- denotes (
T39382 7428-7429 CD denotes 2
T39381 7430-7432 NN denotes μg
T39383 7432-7433 SYM denotes /
T39384 7433-7434 NN denotes g
T39385 7435-7437 IN denotes of
T39386 7438-7442 NN denotes body
T39387 7443-7449 NN denotes weight
T39388 7449-7450 -RRB- denotes )
T39389 7451-7452 -LRB- denotes [
T39390 7452-7454 CD denotes 45
T39391 7454-7455 -RRB- denotes ]
T39392 7455-7456 . denotes .
T39393 7456-7585 sentence denotes Postagonist assessment of oxygen consumption was recorded for an additional 1.0 h, with data collected at the 40-min time point.
T39394 7457-7468 NN denotes Postagonist
T39395 7469-7479 NN denotes assessment
T39397 7480-7482 IN denotes of
T39398 7483-7489 NN denotes oxygen
T39399 7490-7501 NN denotes consumption
T39400 7502-7505 VBD denotes was
T39396 7506-7514 VBN denotes recorded
T39401 7515-7518 IN denotes for
T39402 7519-7521 DT denotes an
T39404 7522-7532 JJ denotes additional
T39405 7533-7536 CD denotes 1.0
T39403 7537-7538 NN denotes h
T39406 7538-7540 , denotes ,
T39407 7540-7544 IN denotes with
T39408 7545-7549 NNS denotes data
T39409 7550-7559 VBN denotes collected
T39410 7560-7562 IN denotes at
T39411 7563-7566 DT denotes the
T39413 7567-7569 CD denotes 40
T39415 7569-7570 HYPH denotes -
T39414 7570-7573 NN denotes min
T39416 7574-7578 NN denotes time
T39412 7579-7584 NN denotes point
T39417 7584-7585 . denotes .
T40046 7587-7596 NN denotes Histology
T40047 7597-7600 CC denotes and
T40048 7601-7609 NN denotes electron
T40049 7610-7620 NN denotes microscopy
T40050 7620-7758 sentence denotes Soleus muscle and liver were dissected and fixed overnight in 2% glutaraldehyde, 1% paraformaldehyde, and 0.08% sodium cacodylate buffer.
T40051 7621-7627 NN denotes Soleus
T40052 7628-7634 NN denotes muscle
T40054 7635-7638 CC denotes and
T40055 7639-7644 NN denotes liver
T40056 7645-7649 VBD denotes were
T40053 7650-7659 VBN denotes dissected
T40057 7660-7663 CC denotes and
T40058 7664-7669 VBN denotes fixed
T40059 7670-7679 RB denotes overnight
T40060 7680-7682 IN denotes in
T40061 7683-7684 CD denotes 2
T40062 7684-7685 NN denotes %
T40063 7686-7700 NN denotes glutaraldehyde
T40065 7700-7702 , denotes ,
T40066 7702-7703 CD denotes 1
T40067 7703-7704 NN denotes %
T40068 7705-7721 NN denotes paraformaldehyde
T40069 7721-7723 , denotes ,
T40070 7723-7726 CC denotes and
T40071 7727-7731 CD denotes 0.08
T40072 7731-7732 NN denotes %
T40073 7733-7739 NN denotes sodium
T40074 7740-7750 NN denotes cacodylate
T40064 7751-7757 NN denotes buffer
T40075 7757-7758 . denotes .
T40076 7758-7914 sentence denotes The tissues were postfixed in 1% osmium tetroxide, dehydrated in graded ethanol, embedded in Poly Bed plastic resin, and sectioned for electron microscopy.
T40077 7759-7762 DT denotes The
T40078 7763-7770 NNS denotes tissues
T40080 7771-7775 VBD denotes were
T40079 7776-7785 VBN denotes postfixed
T40081 7786-7788 IN denotes in
T40082 7789-7790 CD denotes 1
T40083 7790-7791 NN denotes %
T40085 7792-7798 NN denotes osmium
T40084 7799-7808 NN denotes tetroxide
T40086 7808-7810 , denotes ,
T40087 7810-7820 VBN denotes dehydrated
T40088 7821-7823 IN denotes in
T40089 7824-7830 JJ denotes graded
T40090 7831-7838 NN denotes ethanol
T40091 7838-7840 , denotes ,
T40092 7840-7848 VBN denotes embedded
T40093 7849-7851 IN denotes in
T40094 7852-7856 NNP denotes Poly
T40095 7857-7860 NNP denotes Bed
T40097 7861-7868 NN denotes plastic
T40096 7869-7874 NN denotes resin
T40098 7874-7876 , denotes ,
T40099 7876-7879 CC denotes and
T40100 7880-7889 VBN denotes sectioned
T40101 7890-7893 IN denotes for
T40102 7894-7902 NN denotes electron
T40103 7903-7913 NN denotes microscopy
T40104 7913-7914 . denotes .
T40105 7914-8062 sentence denotes Cardiac and skeletal muscle mitochondrial and myofibrillar volume densities were determined from electron micrographs as described previously [10].
T40106 7915-7922 JJ denotes Cardiac
T40108 7923-7926 CC denotes and
T40109 7927-7935 JJ denotes skeletal
T40107 7936-7942 NN denotes muscle
T40111 7943-7956 JJ denotes mitochondrial
T40112 7957-7960 CC denotes and
T40113 7961-7973 JJ denotes myofibrillar
T40114 7974-7980 NN denotes volume
T40110 7981-7990 NNS denotes densities
T40116 7991-7995 VBD denotes were
T40115 7996-8006 VBN denotes determined
T40117 8007-8011 IN denotes from
T40118 8012-8020 NN denotes electron
T40119 8021-8032 NNS denotes micrographs
T40120 8033-8035 IN denotes as
T40121 8036-8045 VBN denotes described
T40122 8046-8056 RB denotes previously
T40123 8057-8058 -LRB- denotes [
T40124 8058-8060 CD denotes 10
T40125 8060-8061 -RRB- denotes ]
T40126 8061-8062 . denotes .
T40127 8062-8168 sentence denotes For each animal, three different fields at the magnification of 7500× were quantified in blinded fashion.
T40128 8063-8066 IN denotes For
T40130 8067-8071 DT denotes each
T40131 8072-8078 NN denotes animal
T40132 8078-8080 , denotes ,
T40133 8080-8085 CD denotes three
T40135 8086-8095 JJ denotes different
T40134 8096-8102 NNS denotes fields
T40136 8103-8105 IN denotes at
T40137 8106-8109 DT denotes the
T40138 8110-8123 NN denotes magnification
T40139 8124-8126 IN denotes of
T40140 8127-8131 NN denotes 7500
T40141 8131-8132 SYM denotes ×
T40142 8133-8137 VBD denotes were
T40129 8138-8148 VBN denotes quantified
T40143 8149-8151 IN denotes in
T40144 8152-8159 JJ denotes blinded
T40145 8160-8167 NN denotes fashion
T40146 8167-8168 . denotes .
T40147 8168-8256 sentence denotes Data were expressed as mean volume density of mitochondria or myofibrils in each field.
T40148 8169-8173 NNS denotes Data
T40150 8174-8178 VBD denotes were
T40149 8179-8188 VBN denotes expressed
T40151 8189-8191 IN denotes as
T40152 8192-8196 NN denotes mean
T40154 8197-8203 NN denotes volume
T40153 8204-8211 NN denotes density
T40155 8212-8214 IN denotes of
T40156 8215-8227 NNS denotes mitochondria
T40157 8228-8230 CC denotes or
T40158 8231-8241 NNS denotes myofibrils
T40159 8242-8244 IN denotes in
T40160 8245-8249 DT denotes each
T40161 8250-8255 NN denotes field
T40162 8255-8256 . denotes .
T40163 8256-8354 sentence denotes For electron microscopic analysis of the brain, tissue was prepared as previously described [46].
T40164 8257-8260 IN denotes For
T40166 8261-8269 NN denotes electron
T40168 8270-8281 JJ denotes microscopic
T40167 8282-8290 NN denotes analysis
T40169 8291-8293 IN denotes of
T40170 8294-8297 DT denotes the
T40171 8298-8303 NN denotes brain
T40172 8303-8305 , denotes ,
T40173 8305-8311 NN denotes tissue
T40174 8312-8315 VBD denotes was
T40165 8316-8324 VBN denotes prepared
T40175 8325-8327 IN denotes as
T40177 8328-8338 RB denotes previously
T40176 8339-8348 VBN denotes described
T40178 8349-8350 -LRB- denotes [
T40179 8350-8352 CD denotes 46
T40180 8352-8353 -RRB- denotes ]
T40181 8353-8354 . denotes .
T40182 8354-8519 sentence denotes Ultrathin sections of cortex were cut onto formvar-coated slot grids stained with uranyl acetate and lead citrate and examined with a JEOL 1200 electron microscope.
T40183 8355-8364 JJ denotes Ultrathin
T40184 8365-8373 NNS denotes sections
T40186 8374-8376 IN denotes of
T40187 8377-8383 NN denotes cortex
T40188 8384-8388 VBD denotes were
T40185 8389-8392 VBN denotes cut
T40189 8393-8397 IN denotes onto
T40190 8398-8405 NN denotes formvar
T40192 8405-8406 HYPH denotes -
T40191 8406-8412 VBN denotes coated
T40194 8413-8417 NN denotes slot
T40193 8418-8423 NNS denotes grids
T40195 8424-8431 VBN denotes stained
T40196 8432-8436 IN denotes with
T40197 8437-8443 NN denotes uranyl
T40198 8444-8451 NN denotes acetate
T40199 8452-8455 CC denotes and
T40200 8456-8460 NN denotes lead
T40201 8461-8468 NN denotes citrate
T40202 8469-8472 CC denotes and
T40203 8473-8481 VBN denotes examined
T40204 8482-8486 IN denotes with
T40205 8487-8488 DT denotes a
T40207 8489-8493 NNP denotes JEOL
T40208 8494-8498 CD denotes 1200
T40209 8499-8507 NN denotes electron
T40206 8508-8518 NN denotes microscope
T40210 8518-8519 . denotes .
T40211 8519-8727 sentence denotes For H&E staining, sections of brain, including cerebral cortex, brainstem, and cerebellum, were dehydrated in graded concentrations of alcohol and embedded in paraffin from which 5-μm sections were prepared.
T40212 8520-8523 IN denotes For
T40214 8524-8525 NN denotes H
T40216 8525-8526 CC denotes &
T40217 8526-8527 NN denotes E
T40215 8528-8536 NN denotes staining
T40218 8536-8538 , denotes ,
T40219 8538-8546 NNS denotes sections
T40220 8547-8549 IN denotes of
T40221 8550-8555 NN denotes brain
T40222 8555-8557 , denotes ,
T40223 8557-8566 VBG denotes including
T40224 8567-8575 JJ denotes cerebral
T40225 8576-8582 NN denotes cortex
T40226 8582-8584 , denotes ,
T40227 8584-8593 NN denotes brainstem
T40228 8593-8595 , denotes ,
T40229 8595-8598 CC denotes and
T40230 8599-8609 NN denotes cerebellum
T40231 8609-8611 , denotes ,
T40232 8611-8615 VBD denotes were
T40213 8616-8626 VBN denotes dehydrated
T40233 8627-8629 IN denotes in
T40234 8630-8636 VBN denotes graded
T40235 8637-8651 NNS denotes concentrations
T40236 8652-8654 IN denotes of
T40237 8655-8662 NN denotes alcohol
T40238 8663-8666 CC denotes and
T40239 8667-8675 VBN denotes embedded
T40240 8676-8678 IN denotes in
T40241 8679-8687 NN denotes paraffin
T40242 8688-8692 IN denotes from
T40244 8693-8698 WDT denotes which
T40245 8699-8700 CD denotes 5
T40247 8700-8701 HYPH denotes -
T40246 8701-8703 NN denotes μm
T40248 8704-8712 NNS denotes sections
T40249 8713-8717 VBD denotes were
T40243 8718-8726 VBN denotes prepared
T40250 8726-8727 . denotes .
T40760 8729-8736 JJ denotes Primary
T40762 8737-8742 NN denotes mouse
T40763 8743-8753 NN denotes hepatocyte
T40761 8754-8761 NNS denotes studies
T40764 8761-8883 sentence denotes Primary cultures of mouse hepatocytes were prepared from male PGC-1α+/+ and PGC-1α−/− mice essentially as described [47].
T40765 8762-8769 JJ denotes Primary
T40766 8770-8778 NNS denotes cultures
T40768 8779-8781 IN denotes of
T40769 8782-8787 NN denotes mouse
T40770 8788-8799 NNS denotes hepatocytes
T40771 8800-8804 VBD denotes were
T40767 8805-8813 VBN denotes prepared
T40772 8814-8818 IN denotes from
T40773 8819-8823 JJ denotes male
T40775 8824-8827 NN denotes PGC
T40777 8827-8828 HYPH denotes -
T40776 8828-8830 NN denotes 1α
T40778 8830-8831 SYM denotes +
T40779 8831-8832 HYPH denotes /
T40780 8832-8833 SYM denotes +
T40781 8834-8837 CC denotes and
T40782 8838-8841 NN denotes PGC
T40784 8841-8842 HYPH denotes -
T40783 8842-8844 NN denotes 1α
T40785 8844-8845 SYM denotes −
T40786 8845-8846 HYPH denotes /
T40787 8846-8847 SYM denotes −
T40774 8848-8852 NNS denotes mice
T40788 8853-8864 RB denotes essentially
T40790 8865-8867 IN denotes as
T40789 8868-8877 VBN denotes described
T40791 8878-8879 -LRB- denotes [
T40792 8879-8881 CD denotes 47
T40793 8881-8882 -RRB- denotes ]
T40794 8882-8883 . denotes .
T40795 8883-8993 sentence denotes Fatty acid oxidation and triglyceride synthesis experiments were commenced 2–3 h after the cells were plated.
T40796 8884-8889 JJ denotes Fatty
T40797 8890-8894 NN denotes acid
T40798 8895-8904 NN denotes oxidation
T40800 8905-8908 CC denotes and
T40801 8909-8921 NN denotes triglyceride
T40802 8922-8931 NN denotes synthesis
T40799 8932-8943 NNS denotes experiments
T40804 8944-8948 VBD denotes were
T40803 8949-8958 VBN denotes commenced
T40805 8959-8960 CD denotes 2
T40807 8960-8961 SYM denotes –
T40806 8961-8962 CD denotes 3
T40808 8963-8964 NN denotes h
T40810 8965-8970 IN denotes after
T40811 8971-8974 DT denotes the
T40812 8975-8980 NNS denotes cells
T40813 8981-8985 VBD denotes were
T40809 8986-8992 VBN denotes plated
T40814 8992-8993 . denotes .
T40815 8993-9070 sentence denotes Triglyceride synthesis studies, were performed as previously described [47].
T40816 8994-9006 NN denotes Triglyceride
T40817 9007-9016 NN denotes synthesis
T40818 9017-9024 NNS denotes studies
T40820 9024-9026 , denotes ,
T40821 9026-9030 VBD denotes were
T40819 9031-9040 VBN denotes performed
T40822 9041-9043 IN denotes as
T40824 9044-9054 RB denotes previously
T40823 9055-9064 VBN denotes described
T40825 9065-9066 -LRB- denotes [
T40826 9066-9068 CD denotes 47
T40827 9068-9069 -RRB- denotes ]
T40828 9069-9070 . denotes .
T40829 9070-9210 sentence denotes Palmitate oxidation rates were quantified using [9,10-3H]-palmitic acid as described [48] and corrected for total cellular protein content.
T40830 9071-9080 NN denotes Palmitate
T40832 9081-9090 NN denotes oxidation
T40831 9091-9096 NNS denotes rates
T40834 9097-9101 VBD denotes were
T40833 9102-9112 VBN denotes quantified
T40835 9113-9118 VBG denotes using
T40836 9119-9120 -LRB- denotes [
T40837 9120-9121 CD denotes 9
T40839 9121-9122 , denotes ,
T40840 9122-9124 CD denotes 10
T40841 9124-9125 SYM denotes -
T40838 9125-9127 NN denotes 3H
T40843 9127-9128 -RRB- denotes ]
T40844 9128-9129 HYPH denotes -
T40842 9129-9137 JJ denotes palmitic
T40845 9138-9142 NN denotes acid
T40846 9143-9145 IN denotes as
T40847 9146-9155 VBN denotes described
T40848 9156-9157 -LRB- denotes [
T40849 9157-9159 CD denotes 48
T40850 9159-9160 -RRB- denotes ]
T40851 9161-9164 CC denotes and
T40852 9165-9174 VBN denotes corrected
T40853 9175-9178 IN denotes for
T40854 9179-9184 JJ denotes total
T40856 9185-9193 JJ denotes cellular
T40857 9194-9201 NN denotes protein
T40855 9202-9209 NN denotes content
T40858 9209-9210 . denotes .
T40859 9210-9365 sentence denotes For respiration studies, cells were spun down prior to plating and resuspended in a permeabilization buffer (described above) containing 50 μg/ml saponin.
T40860 9211-9214 IN denotes For
T40862 9215-9226 NN denotes respiration
T40863 9227-9234 NNS denotes studies
T40864 9234-9236 , denotes ,
T40865 9236-9241 NNS denotes cells
T40866 9242-9246 VBD denotes were
T40861 9247-9251 VBN denotes spun
T40867 9252-9256 RP denotes down
T40868 9257-9262 JJ denotes prior
T40869 9263-9265 IN denotes to
T40870 9266-9273 NN denotes plating
T40871 9274-9277 CC denotes and
T40872 9278-9289 VBN denotes resuspended
T40873 9290-9292 IN denotes in
T40874 9293-9294 DT denotes a
T40876 9295-9311 NN denotes permeabilization
T40875 9312-9318 NN denotes buffer
T40877 9319-9320 -LRB- denotes (
T40878 9320-9329 VBN denotes described
T40879 9330-9335 RB denotes above
T40880 9335-9336 -RRB- denotes )
T40881 9337-9347 VBG denotes containing
T40882 9348-9350 CD denotes 50
T40883 9351-9353 NN denotes μg
T40885 9353-9354 SYM denotes /
T40886 9354-9356 NN denotes ml
T40884 9357-9364 NN denotes saponin
T40887 9364-9365 . denotes .
T40888 9365-9469 sentence denotes Respiration studies were performed in the presence of 5 mM succinate in the presence of 10 μM rotenone.
T40889 9366-9377 NN denotes Respiration
T40890 9378-9385 NNS denotes studies
T40892 9386-9390 VBD denotes were
T40891 9391-9400 VBN denotes performed
T40893 9401-9403 IN denotes in
T40894 9404-9407 DT denotes the
T40895 9408-9416 NN denotes presence
T40896 9417-9419 IN denotes of
T40897 9420-9421 CD denotes 5
T40898 9422-9424 NN denotes mM
T40899 9425-9434 NN denotes succinate
T40900 9435-9437 IN denotes in
T40901 9438-9441 DT denotes the
T40902 9442-9450 NN denotes presence
T40903 9451-9453 IN denotes of
T40904 9454-9456 CD denotes 10
T40905 9457-9459 NN denotes μM
T40906 9460-9468 NN denotes rotenone
T40907 9468-9469 . denotes .
T40908 9469-9540 sentence denotes Respiration rates were expressed as nmol O2 · min−1 · mg of protein−1.
T40909 9470-9481 NN denotes Respiration
T40910 9482-9487 NNS denotes rates
T40912 9488-9492 VBD denotes were
T40911 9493-9502 VBN denotes expressed
T40913 9503-9505 IN denotes as
T40914 9506-9510 NN denotes nmol
T40915 9511-9513 NN denotes O2
T40916 9514-9515 SYM denotes ·
T40917 9516-9519 NN denotes min
T40918 9519-9520 SYM denotes −
T40919 9520-9521 CD denotes 1
T40920 9522-9523 SYM denotes ·
T40921 9524-9526 NN denotes mg
T40922 9527-9529 IN denotes of
T40923 9530-9537 NN denotes protein
T40924 9537-9538 SYM denotes −
T40925 9538-9539 CD denotes 1
T40926 9539-9540 . denotes .
T42846 9542-9552 NN denotes Evaluation
T42847 9553-9555 IN denotes of
T42848 9556-9565 NN denotes locomotor
T42849 9566-9574 NN denotes activity
T42850 9574-9576 , denotes ,
T42851 9576-9588 NN denotes sensorimotor
T42852 9589-9601 NNS denotes capabilities
T42853 9601-9603 , denotes ,
T42854 9603-9606 CC denotes and
T42855 9607-9613 NN denotes muscle
T42856 9614-9622 NN denotes function
T42857 9622-9918 sentence denotes To evaluate general activity levels and muscle use, mice were evaluated over a 1-h period in transparent (47.6 cm × 25.4 cm × 20.6 cm) polystyrene enclosures as previously described [49] using a high-resolution photobeam system (Motor Monitor, Hamilton-Kinder, Poway, California, United States).
T42858 9623-9625 TO denotes To
T42859 9626-9634 VB denotes evaluate
T42861 9635-9642 JJ denotes general
T42863 9643-9651 NN denotes activity
T42862 9652-9658 NNS denotes levels
T42864 9659-9662 CC denotes and
T42865 9663-9669 NN denotes muscle
T42866 9670-9673 NN denotes use
T42867 9673-9675 , denotes ,
T42868 9675-9679 NNS denotes mice
T42869 9680-9684 VBD denotes were
T42860 9685-9694 VBN denotes evaluated
T42870 9695-9699 IN denotes over
T42871 9700-9701 DT denotes a
T42873 9702-9703 CD denotes 1
T42875 9703-9704 HYPH denotes -
T42874 9704-9705 NN denotes h
T42872 9706-9712 NN denotes period
T42876 9713-9715 IN denotes in
T42877 9716-9727 NN denotes transparent
T42879 9728-9729 -LRB- denotes (
T42880 9729-9733 CD denotes 47.6
T42881 9734-9736 NN denotes cm
T42882 9737-9738 SYM denotes ×
T42884 9739-9743 CD denotes 25.4
T42883 9744-9746 NN denotes cm
T42885 9747-9748 SYM denotes ×
T42887 9749-9753 CD denotes 20.6
T42886 9754-9756 NN denotes cm
T42888 9756-9757 -RRB- denotes )
T42878 9758-9769 NN denotes polystyrene
T42889 9770-9780 VBZ denotes enclosures
T42890 9781-9783 IN denotes as
T42892 9784-9794 RB denotes previously
T42891 9795-9804 VBN denotes described
T42893 9805-9806 -LRB- denotes [
T42894 9806-9808 CD denotes 49
T42895 9808-9809 -RRB- denotes ]
T42896 9810-9815 VBG denotes using
T42897 9816-9817 DT denotes a
T42899 9818-9822 JJ denotes high
T42901 9822-9823 HYPH denotes -
T42900 9823-9833 NN denotes resolution
T42902 9834-9843 NN denotes photobeam
T42898 9844-9850 NN denotes system
T42903 9851-9852 -LRB- denotes (
T42905 9852-9857 NNP denotes Motor
T42904 9858-9865 NNP denotes Monitor
T42906 9865-9867 , denotes ,
T42907 9867-9875 NNP denotes Hamilton
T42909 9875-9876 HYPH denotes -
T42908 9876-9882 NNP denotes Kinder
T42910 9882-9884 , denotes ,
T42911 9884-9889 NNP denotes Poway
T42912 9889-9891 , denotes ,
T42913 9891-9901 NNP denotes California
T42914 9901-9903 , denotes ,
T42915 9903-9909 NNP denotes United
T42916 9910-9916 NNP denotes States
T42917 9916-9917 -RRB- denotes )
T42918 9917-9918 . denotes .
T42919 9918-10057 sentence denotes Each enclosure was surrounded by a frame containing a 4 × 8 matrix of photocell pairs, the output of which was fed to an on-line computer.
T42920 9919-9923 DT denotes Each
T42921 9924-9933 NN denotes enclosure
T42923 9934-9937 VBD denotes was
T42922 9938-9948 VBN denotes surrounded
T42924 9949-9951 IN denotes by
T42925 9952-9953 DT denotes a
T42926 9954-9959 NN denotes frame
T42927 9960-9970 VBG denotes containing
T42928 9971-9972 DT denotes a
T42930 9973-9974 CD denotes 4
T42931 9975-9976 SYM denotes ×
T42932 9977-9978 CD denotes 8
T42929 9979-9985 NN denotes matrix
T42933 9986-9988 IN denotes of
T42934 9989-9998 NN denotes photocell
T42935 9999-10004 NNS denotes pairs
T42936 10004-10006 , denotes ,
T42937 10006-10009 DT denotes the
T42938 10010-10016 NN denotes output
T42940 10017-10019 IN denotes of
T42941 10020-10025 WDT denotes which
T42942 10026-10029 VBD denotes was
T42939 10030-10033 VBN denotes fed
T42943 10034-10036 IN denotes to
T42944 10037-10039 DT denotes an
T42946 10040-10042 IN denotes on
T42947 10042-10043 HYPH denotes -
T42948 10043-10047 NN denotes line
T42945 10048-10056 NN denotes computer
T42949 10056-10057 . denotes .
T42950 10057-10258 sentence denotes The system software (Hamilton-Kinder) was used to define a 33 cm × 11 cm central zone and a peripheral or surrounding zone that was 5.5 cm wide with the sides of the cage being the outermost boundary.
T42951 10058-10061 DT denotes The
T42953 10062-10068 NN denotes system
T42952 10069-10077 NN denotes software
T42955 10078-10079 -LRB- denotes (
T42957 10079-10087 NNP denotes Hamilton
T42958 10087-10088 HYPH denotes -
T42956 10088-10094 NNP denotes Kinder
T42959 10094-10095 -RRB- denotes )
T42960 10096-10099 VBD denotes was
T42954 10100-10104 VBN denotes used
T42961 10105-10107 TO denotes to
T42962 10108-10114 VB denotes define
T42963 10115-10116 DT denotes a
T42965 10117-10119 CD denotes 33
T42966 10120-10122 NN denotes cm
T42967 10123-10124 SYM denotes ×
T42969 10125-10127 CD denotes 11
T42968 10128-10130 NN denotes cm
T42970 10131-10138 JJ denotes central
T42964 10139-10143 NN denotes zone
T42971 10144-10147 CC denotes and
T42972 10148-10149 DT denotes a
T42974 10150-10160 JJ denotes peripheral
T42975 10161-10163 CC denotes or
T42976 10164-10175 VBG denotes surrounding
T42973 10176-10180 NN denotes zone
T42977 10181-10185 WDT denotes that
T42978 10186-10189 VBD denotes was
T42979 10190-10193 CD denotes 5.5
T42980 10194-10196 NN denotes cm
T42981 10197-10201 JJ denotes wide
T42982 10202-10206 IN denotes with
T42983 10207-10210 DT denotes the
T42984 10211-10216 NNS denotes sides
T42986 10217-10219 IN denotes of
T42987 10220-10223 DT denotes the
T42988 10224-10228 NN denotes cage
T42985 10229-10234 VBG denotes being
T42989 10235-10238 DT denotes the
T42991 10239-10248 JJ denotes outermost
T42990 10249-10257 NN denotes boundary
T42992 10257-10258 . denotes .
T42993 10258-10328 sentence denotes This peripheral area extended along the entire perimeter of the cage.
T42994 10259-10263 DT denotes This
T42996 10264-10274 JJ denotes peripheral
T42995 10275-10279 NN denotes area
T42997 10280-10288 VBD denotes extended
T42998 10289-10294 IN denotes along
T42999 10295-10298 DT denotes the
T43001 10299-10305 JJ denotes entire
T43000 10306-10315 NN denotes perimeter
T43002 10316-10318 IN denotes of
T43003 10319-10322 DT denotes the
T43004 10323-10327 NN denotes cage
T43005 10327-10328 . denotes .
T43006 10328-10568 sentence denotes Variables that were analyzed included the total number of ambulations, as well as the number of entries, the time spent, and the distance traveled in the center area as well as the distance traveled in the periphery surrounding the center.
T43007 10329-10338 NNS denotes Variables
T43009 10339-10343 WDT denotes that
T43011 10344-10348 VBD denotes were
T43010 10349-10357 VBN denotes analyzed
T43008 10358-10366 VBD denotes included
T43012 10367-10370 DT denotes the
T43014 10371-10376 JJ denotes total
T43013 10377-10383 NN denotes number
T43015 10384-10386 IN denotes of
T43016 10387-10398 NNS denotes ambulations
T43017 10398-10400 , denotes ,
T43018 10400-10402 RB denotes as
T43020 10403-10407 RB denotes well
T43019 10408-10410 IN denotes as
T43021 10411-10414 DT denotes the
T43022 10415-10421 NN denotes number
T43023 10422-10424 IN denotes of
T43024 10425-10432 NNS denotes entries
T43025 10432-10434 , denotes ,
T43026 10434-10437 DT denotes the
T43027 10438-10442 NN denotes time
T43028 10443-10448 VBN denotes spent
T43029 10448-10450 , denotes ,
T43030 10450-10453 CC denotes and
T43031 10454-10457 DT denotes the
T43032 10458-10466 NN denotes distance
T43033 10467-10475 VBN denotes traveled
T43034 10476-10478 IN denotes in
T43035 10479-10482 DT denotes the
T43037 10483-10489 NN denotes center
T43036 10490-10494 NN denotes area
T43038 10495-10497 RB denotes as
T43040 10498-10502 RB denotes well
T43039 10503-10505 IN denotes as
T43041 10506-10509 DT denotes the
T43042 10510-10518 NN denotes distance
T43043 10519-10527 VBN denotes traveled
T43044 10528-10530 IN denotes in
T43045 10531-10534 DT denotes the
T43046 10535-10544 NN denotes periphery
T43047 10545-10556 VBG denotes surrounding
T43048 10557-10560 DT denotes the
T43049 10561-10567 NN denotes center
T43050 10567-10568 . denotes .
T43051 10568-10628 sentence denotes The total number of ambulations and rearings were recorded.
T43052 10569-10572 DT denotes The
T43054 10573-10578 JJ denotes total
T43053 10579-10585 NN denotes number
T43056 10586-10588 IN denotes of
T43057 10589-10600 NNS denotes ambulations
T43058 10601-10604 CC denotes and
T43059 10605-10613 NNS denotes rearings
T43060 10614-10618 VBD denotes were
T43055 10619-10627 VBN denotes recorded
T43061 10627-10628 . denotes .
T43062 10628-10755 sentence denotes For the inverted screen test, mice were placed on a wire mesh grid (16 squares per 10 cm) and the screen was inverted to 180°.
T43063 10629-10632 IN denotes For
T43065 10633-10636 DT denotes the
T43067 10637-10645 VBN denotes inverted
T43068 10646-10652 NN denotes screen
T43066 10653-10657 NN denotes test
T43069 10657-10659 , denotes ,
T43070 10659-10663 NNS denotes mice
T43071 10664-10668 VBD denotes were
T43064 10669-10675 VBN denotes placed
T43072 10676-10678 IN denotes on
T43073 10679-10680 DT denotes a
T43075 10681-10685 NN denotes wire
T43076 10686-10690 NN denotes mesh
T43074 10691-10695 NN denotes grid
T43077 10696-10697 -LRB- denotes (
T43079 10697-10699 CD denotes 16
T43078 10700-10707 NNS denotes squares
T43080 10708-10711 IN denotes per
T43081 10712-10714 CD denotes 10
T43082 10715-10717 NN denotes cm
T43083 10717-10718 -RRB- denotes )
T43084 10719-10722 CC denotes and
T43085 10723-10726 DT denotes the
T43086 10727-10733 NN denotes screen
T43088 10734-10737 VBD denotes was
T43087 10738-10746 VBN denotes inverted
T43089 10747-10749 IN denotes to
T43090 10750-10753 CD denotes 180
T43091 10753-10754 NN denotes °
T43092 10754-10755 . denotes .
T43093 10755-10816 sentence denotes A maximum score of 60 s was given if an animal did not fall.
T43094 10756-10757 DT denotes A
T43096 10758-10765 JJ denotes maximum
T43095 10766-10771 NN denotes score
T43098 10772-10774 IN denotes of
T43099 10775-10777 CD denotes 60
T43100 10778-10779 NN denotes s
T43101 10780-10783 VBD denotes was
T43097 10784-10789 VBN denotes given
T43102 10790-10792 IN denotes if
T43104 10793-10795 DT denotes an
T43105 10796-10802 NN denotes animal
T43106 10803-10806 VBD denotes did
T43107 10807-10810 RB denotes not
T43103 10811-10815 VB denotes fall
T43108 10815-10816 . denotes .
T43109 10816-10921 sentence denotes The tests included in the sensorimotor battery [50] and accompanying protocols were designed as follows.
T43110 10817-10820 DT denotes The
T43111 10821-10826 NNS denotes tests
T43113 10827-10835 VBN denotes included
T43114 10836-10838 IN denotes in
T43115 10839-10842 DT denotes the
T43117 10843-10855 NN denotes sensorimotor
T43116 10856-10863 NN denotes battery
T43118 10864-10865 -LRB- denotes [
T43119 10865-10867 CD denotes 50
T43120 10867-10868 -RRB- denotes ]
T43121 10869-10872 CC denotes and
T43122 10873-10885 VBG denotes accompanying
T43123 10886-10895 NNS denotes protocols
T43124 10896-10900 VBD denotes were
T43112 10901-10909 VBN denotes designed
T43125 10910-10912 IN denotes as
T43126 10913-10920 VBZ denotes follows
T43127 10920-10921 . denotes .
T43128 10921-11138 sentence denotes (1) Inclined screen and inverted screen tests: For the inclined screen tests, each mouse was placed on top of an elevated (47 cm above the floor) wire mesh grid (16 squares per 10 cm) that was inclined to 60° or 90°.
T43129 10922-10923 -LRB- denotes (
T43130 10923-10924 LS denotes 1
T43132 10924-10925 -RRB- denotes )
T43133 10926-10934 VBN denotes Inclined
T43134 10935-10941 NN denotes screen
T43136 10942-10945 CC denotes and
T43137 10946-10954 VBN denotes inverted
T43138 10955-10961 NN denotes screen
T43135 10962-10967 NNS denotes tests
T43139 10967-10969 : denotes :
T43140 10969-10972 IN denotes For
T43141 10973-10976 DT denotes the
T43143 10977-10985 VBN denotes inclined
T43144 10986-10992 NN denotes screen
T43142 10993-10998 NNS denotes tests
T43145 10998-11000 , denotes ,
T43146 11000-11004 DT denotes each
T43147 11005-11010 NN denotes mouse
T43148 11011-11014 VBD denotes was
T43131 11015-11021 VBN denotes placed
T43149 11022-11024 IN denotes on
T43150 11025-11028 NN denotes top
T43151 11029-11031 IN denotes of
T43152 11032-11034 DT denotes an
T43154 11035-11043 JJ denotes elevated
T43155 11044-11045 -LRB- denotes (
T43157 11045-11047 CD denotes 47
T43158 11048-11050 NN denotes cm
T43156 11051-11056 IN denotes above
T43159 11057-11060 DT denotes the
T43160 11061-11066 NN denotes floor
T43161 11066-11067 -RRB- denotes )
T43162 11068-11072 NN denotes wire
T43163 11073-11077 NN denotes mesh
T43153 11078-11082 NN denotes grid
T43164 11083-11084 -LRB- denotes (
T43166 11084-11086 CD denotes 16
T43165 11087-11094 NNS denotes squares
T43167 11095-11098 IN denotes per
T43168 11099-11101 CD denotes 10
T43169 11102-11104 NN denotes cm
T43170 11104-11105 -RRB- denotes )
T43171 11106-11110 WDT denotes that
T43173 11111-11114 VBD denotes was
T43172 11115-11123 VBN denotes inclined
T43174 11124-11126 IN denotes to
T43175 11127-11129 CD denotes 60
T43176 11129-11130 NN denotes °
T43177 11131-11133 CC denotes or
T43178 11134-11136 CD denotes 90
T43179 11136-11137 NN denotes °
T43180 11137-11138 . denotes .
T43181 11138-11326 sentence denotes Each animal was placed in the middle of the screen with its head oriented down and was timed for how long it remained on the screen and how long it took to climb to the top of the screen.
T43182 11139-11143 DT denotes Each
T43183 11144-11150 NN denotes animal
T43185 11151-11154 VBD denotes was
T43184 11155-11161 VBN denotes placed
T43186 11162-11164 IN denotes in
T43187 11165-11168 DT denotes the
T43188 11169-11175 NN denotes middle
T43189 11176-11178 IN denotes of
T43190 11179-11182 DT denotes the
T43191 11183-11189 NN denotes screen
T43192 11190-11194 IN denotes with
T43194 11195-11198 PRP$ denotes its
T43195 11199-11203 NN denotes head
T43193 11204-11212 VBN denotes oriented
T43196 11213-11217 RB denotes down
T43197 11218-11221 CC denotes and
T43198 11222-11225 VBD denotes was
T43199 11226-11231 VBN denotes timed
T43200 11232-11235 IN denotes for
T43201 11236-11239 WRB denotes how
T43202 11240-11244 RB denotes long
T43204 11245-11247 PRP denotes it
T43203 11248-11256 VBD denotes remained
T43205 11257-11259 IN denotes on
T43206 11260-11263 DT denotes the
T43207 11264-11270 NN denotes screen
T43208 11271-11274 CC denotes and
T43209 11275-11278 WRB denotes how
T43210 11279-11283 RB denotes long
T43212 11284-11286 PRP denotes it
T43211 11287-11291 VBD denotes took
T43213 11292-11294 TO denotes to
T43214 11295-11300 VB denotes climb
T43215 11301-11303 IN denotes to
T43216 11304-11307 DT denotes the
T43217 11308-11311 NN denotes top
T43218 11312-11314 IN denotes of
T43219 11315-11318 DT denotes the
T43220 11319-11325 NN denotes screen
T43221 11325-11326 . denotes .
T43222 11326-11424 sentence denotes For the inverted screen test, mice were placed as above and then the screen was inverted to 180°.
T43223 11327-11330 IN denotes For
T43225 11331-11334 DT denotes the
T43227 11335-11343 VBN denotes inverted
T43228 11344-11350 NN denotes screen
T43226 11351-11355 NN denotes test
T43229 11355-11357 , denotes ,
T43230 11357-11361 NNS denotes mice
T43231 11362-11366 VBD denotes were
T43224 11367-11373 VBN denotes placed
T43232 11374-11376 IN denotes as
T43233 11377-11382 RB denotes above
T43234 11383-11386 CC denotes and
T43235 11387-11391 RB denotes then
T43237 11392-11395 DT denotes the
T43238 11396-11402 NN denotes screen
T43239 11403-11406 VBD denotes was
T43236 11407-11415 VBN denotes inverted
T43240 11416-11418 IN denotes to
T43241 11419-11422 CD denotes 180
T43242 11422-11423 NN denotes °
T43243 11423-11424 . denotes .
T43244 11424-11485 sentence denotes A maximum score of 60 s was given if an animal did not fall;
T43245 11425-11426 DT denotes A
T43247 11427-11434 JJ denotes maximum
T43246 11435-11440 NN denotes score
T43249 11441-11443 IN denotes of
T43250 11444-11446 CD denotes 60
T43251 11447-11448 NN denotes s
T43252 11449-11452 VBD denotes was
T43248 11453-11458 VBN denotes given
T43253 11459-11461 IN denotes if
T43255 11462-11464 DT denotes an
T43256 11465-11471 NN denotes animal
T43257 11472-11475 VBD denotes did
T43258 11476-11479 RB denotes not
T43254 11480-11484 VB denotes fall
T43259 11484-11485 : denotes ;
T43260 11485-11646 sentence denotes (2) Platform test: Each mouse was timed for how long it remained on an elevated (47 cm above the floor) circular platform (1.0 cm thick and 3.0 cm in diameter).
T43261 11486-11487 -LRB- denotes (
T43262 11487-11488 LS denotes 2
T43264 11488-11489 -RRB- denotes )
T43265 11490-11498 NN denotes Platform
T43266 11499-11503 NN denotes test
T43267 11503-11505 : denotes :
T43268 11505-11509 DT denotes Each
T43269 11510-11515 NN denotes mouse
T43270 11516-11519 VBD denotes was
T43263 11520-11525 VBN denotes timed
T43271 11526-11529 IN denotes for
T43272 11530-11533 WRB denotes how
T43273 11534-11538 RB denotes long
T43275 11539-11541 PRP denotes it
T43274 11542-11550 VBD denotes remained
T43276 11551-11553 IN denotes on
T43277 11554-11556 DT denotes an
T43279 11557-11565 JJ denotes elevated
T43280 11566-11567 -LRB- denotes (
T43282 11567-11569 CD denotes 47
T43283 11570-11572 NN denotes cm
T43281 11573-11578 IN denotes above
T43284 11579-11582 DT denotes the
T43285 11583-11588 NN denotes floor
T43286 11588-11589 -RRB- denotes )
T43287 11590-11598 JJ denotes circular
T43278 11599-11607 NN denotes platform
T43288 11608-11609 -LRB- denotes (
T43290 11609-11612 CD denotes 1.0
T43291 11613-11615 NN denotes cm
T43289 11616-11621 JJ denotes thick
T43292 11622-11625 CC denotes and
T43293 11626-11629 CD denotes 3.0
T43294 11630-11632 NN denotes cm
T43295 11633-11635 IN denotes in
T43296 11636-11644 NN denotes diameter
T43297 11644-11645 -RRB- denotes )
T43298 11645-11646 . denotes .
T43299 11646-11844 sentence denotes A maximum score of 60 s was assigned if the mouse remained on the platform for the maximum amount of time or if it could climb down on a very thin pole that supported the platform, without falling;
T43300 11647-11648 DT denotes A
T43302 11649-11656 JJ denotes maximum
T43301 11657-11662 NN denotes score
T43304 11663-11665 IN denotes of
T43305 11666-11668 CD denotes 60
T43306 11669-11670 NN denotes s
T43307 11671-11674 VBD denotes was
T43303 11675-11683 VBN denotes assigned
T43308 11684-11686 IN denotes if
T43310 11687-11690 DT denotes the
T43311 11691-11696 NN denotes mouse
T43309 11697-11705 VBD denotes remained
T43312 11706-11708 IN denotes on
T43313 11709-11712 DT denotes the
T43314 11713-11721 NN denotes platform
T43315 11722-11725 IN denotes for
T43316 11726-11729 DT denotes the
T43318 11730-11737 NN denotes maximum
T43317 11738-11744 NN denotes amount
T43319 11745-11747 IN denotes of
T43320 11748-11752 NN denotes time
T43321 11753-11755 CC denotes or
T43322 11756-11758 IN denotes if
T43324 11759-11761 PRP denotes it
T43325 11762-11767 MD denotes could
T43323 11768-11773 VB denotes climb
T43326 11774-11778 RB denotes down
T43327 11779-11781 IN denotes on
T43328 11782-11783 DT denotes a
T43330 11784-11788 RB denotes very
T43331 11789-11793 JJ denotes thin
T43329 11794-11798 NN denotes pole
T43332 11799-11803 WDT denotes that
T43333 11804-11813 VBD denotes supported
T43334 11814-11817 DT denotes the
T43335 11818-11826 NN denotes platform
T43336 11826-11828 , denotes ,
T43337 11828-11835 IN denotes without
T43338 11836-11843 VBG denotes falling
T43339 11843-11844 : denotes ;
T43340 11844-11990 sentence denotes (3) Ledge test: Each mouse was timed for how long it could maintain its balance on a 0.75-cm wide Plexiglas ledge without falling (60 s maximum).
T43341 11845-11846 -LRB- denotes (
T43342 11846-11847 LS denotes 3
T43344 11847-11848 -RRB- denotes )
T43345 11849-11854 NN denotes Ledge
T43346 11855-11859 NN denotes test
T43347 11859-11861 : denotes :
T43348 11861-11865 DT denotes Each
T43349 11866-11871 NN denotes mouse
T43350 11872-11875 VBD denotes was
T43343 11876-11881 VBN denotes timed
T43351 11882-11885 IN denotes for
T43352 11886-11889 WRB denotes how
T43353 11890-11894 RB denotes long
T43355 11895-11897 PRP denotes it
T43356 11898-11903 MD denotes could
T43354 11904-11912 VB denotes maintain
T43357 11913-11916 PRP$ denotes its
T43358 11917-11924 NN denotes balance
T43359 11925-11927 IN denotes on
T43360 11928-11929 DT denotes a
T43362 11930-11934 CD denotes 0.75
T43364 11934-11935 HYPH denotes -
T43363 11935-11937 NN denotes cm
T43365 11938-11942 JJ denotes wide
T43366 11943-11952 NN denotes Plexiglas
T43361 11953-11958 NN denotes ledge
T43367 11959-11966 IN denotes without
T43368 11967-11974 VBG denotes falling
T43369 11975-11976 -LRB- denotes (
T43371 11976-11978 CD denotes 60
T43372 11979-11980 NN denotes s
T43370 11981-11988 NN denotes maximum
T43373 11988-11989 -RRB- denotes )
T43374 11989-11990 . denotes .
T43375 11990-12166 sentence denotes A score of 60 s was also assigned if the mouse traversed the entire length (51 cm) of the Plexiglas ledge and returned to the starting place in less than 60 s without falling;
T43376 11991-11992 DT denotes A
T43377 11993-11998 NN denotes score
T43379 11999-12001 IN denotes of
T43380 12002-12004 CD denotes 60
T43381 12005-12006 NN denotes s
T43382 12007-12010 VBD denotes was
T43383 12011-12015 RB denotes also
T43378 12016-12024 VBN denotes assigned
T43384 12025-12027 IN denotes if
T43386 12028-12031 DT denotes the
T43387 12032-12037 NN denotes mouse
T43385 12038-12047 VBD denotes traversed
T43388 12048-12051 DT denotes the
T43390 12052-12058 JJ denotes entire
T43389 12059-12065 NN denotes length
T43391 12066-12067 -LRB- denotes (
T43392 12067-12069 CD denotes 51
T43393 12070-12072 NN denotes cm
T43394 12072-12073 -RRB- denotes )
T43395 12074-12076 IN denotes of
T43396 12077-12080 DT denotes the
T43398 12081-12090 NN denotes Plexiglas
T43397 12091-12096 NN denotes ledge
T43399 12097-12100 CC denotes and
T43400 12101-12109 VBD denotes returned
T43401 12110-12112 IN denotes to
T43402 12113-12116 DT denotes the
T43404 12117-12125 NN denotes starting
T43403 12126-12131 NN denotes place
T43405 12132-12134 IN denotes in
T43406 12135-12139 JJR denotes less
T43408 12140-12144 IN denotes than
T43407 12145-12147 CD denotes 60
T43409 12148-12149 NN denotes s
T43410 12150-12157 IN denotes without
T43411 12158-12165 VBG denotes falling
T43412 12165-12166 : denotes ;
T43413 12166-12335 sentence denotes (4) Walking initiation test: Each mouse was placed in the middle of a square outlined by white cloth tape (21 cm × 21 cm) on a smooth black surface of a large tabletop.
T43414 12167-12168 -LRB- denotes (
T43415 12168-12169 LS denotes 4
T43417 12169-12170 -RRB- denotes )
T43418 12171-12178 NN denotes Walking
T43419 12179-12189 NN denotes initiation
T43416 12190-12194 NN denotes test
T43420 12194-12196 : denotes :
T43421 12196-12200 DT denotes Each
T43422 12201-12206 NN denotes mouse
T43424 12207-12210 VBD denotes was
T43423 12211-12217 VBN denotes placed
T43425 12218-12220 IN denotes in
T43426 12221-12224 DT denotes the
T43427 12225-12231 NN denotes middle
T43428 12232-12234 IN denotes of
T43429 12235-12236 DT denotes a
T43430 12237-12243 NN denotes square
T43431 12244-12252 VBN denotes outlined
T43432 12253-12255 IN denotes by
T43433 12256-12261 JJ denotes white
T43435 12262-12267 NN denotes cloth
T43434 12268-12272 NN denotes tape
T43436 12273-12274 -LRB- denotes (
T43438 12274-12276 CD denotes 21
T43437 12277-12279 NN denotes cm
T43439 12280-12281 SYM denotes ×
T43441 12282-12284 CD denotes 21
T43440 12285-12287 NN denotes cm
T43442 12287-12288 -RRB- denotes )
T43443 12289-12291 IN denotes on
T43444 12292-12293 DT denotes a
T43446 12294-12300 JJ denotes smooth
T43447 12301-12306 JJ denotes black
T43445 12307-12314 NN denotes surface
T43448 12315-12317 IN denotes of
T43449 12318-12319 DT denotes a
T43451 12320-12325 JJ denotes large
T43450 12326-12334 NN denotes tabletop
T43452 12334-12335 CC denotes .
T43453 12335-12439 sentence denotes The time it took each mouse to leave the square (place all four paws outside of the tape) was recorded.
T43454 12336-12339 DT denotes The
T43455 12340-12344 NN denotes time
T43457 12345-12347 PRP denotes it
T43458 12348-12352 VBD denotes took
T43459 12353-12357 DT denotes each
T43460 12358-12363 NN denotes mouse
T43462 12364-12366 TO denotes to
T43461 12367-12372 VB denotes leave
T43463 12373-12376 DT denotes the
T43464 12377-12383 NN denotes square
T43465 12384-12385 -LRB- denotes (
T43466 12385-12390 VB denotes place
T43467 12391-12394 DT denotes all
T43469 12395-12399 CD denotes four
T43468 12400-12404 NNS denotes paws
T43470 12405-12412 RB denotes outside
T43471 12413-12415 IN denotes of
T43472 12416-12419 DT denotes the
T43473 12420-12424 NN denotes tape
T43474 12424-12425 -RRB- denotes )
T43475 12426-12429 VBD denotes was
T43456 12430-12438 VBN denotes recorded
T43476 12438-12439 . denotes .
T43477 12439-12474 sentence denotes The maximum time allowed was 60 s.
T43478 12440-12443 DT denotes The
T43480 12444-12451 JJ denotes maximum
T43479 12452-12456 NN denotes time
T43482 12457-12464 VBN denotes allowed
T43481 12465-12468 VBD denotes was
T43483 12469-12471 CD denotes 60
T43484 12472-12473 NN denotes s
T43485 12473-12474 . denotes .
T43486 12474-12643 sentence denotes 6–8-mo-old PGC-1α+/+ (n = 4) and PGC-1α−/− (n = 8) mice were run to exhaustion employing a motorized, speed controlled, modular treadmill system (Columbus Instruments).
T43487 12475-12476 CD denotes 6
T43489 12476-12477 SYM denotes –
T43488 12477-12478 CD denotes 8
T43491 12478-12479 HYPH denotes -
T43490 12479-12481 NN denotes mo
T43493 12481-12482 HYPH denotes -
T43492 12482-12485 JJ denotes old
T43495 12486-12489 NN denotes PGC
T43497 12489-12490 HYPH denotes -
T43496 12490-12492 NN denotes 1α
T43498 12492-12493 SYM denotes +
T43499 12493-12494 HYPH denotes /
T43500 12494-12495 SYM denotes +
T43501 12496-12497 -LRB- denotes (
T43503 12497-12498 NN denotes n
T43504 12499-12500 SYM denotes =
T43502 12501-12502 CD denotes 4
T43505 12502-12503 -RRB- denotes )
T43506 12504-12507 CC denotes and
T43507 12508-12511 NN denotes PGC
T43509 12511-12512 HYPH denotes -
T43508 12512-12514 NN denotes 1α
T43510 12514-12515 SYM denotes −
T43511 12515-12516 HYPH denotes /
T43512 12516-12517 SYM denotes −
T43513 12518-12519 -LRB- denotes (
T43515 12519-12520 NN denotes n
T43516 12521-12522 SYM denotes =
T43514 12523-12524 CD denotes 8
T43517 12524-12525 -RRB- denotes )
T43494 12526-12530 NNS denotes mice
T43519 12531-12535 VBD denotes were
T43518 12536-12539 VBN denotes run
T43520 12540-12542 IN denotes to
T43521 12543-12553 NN denotes exhaustion
T43522 12554-12563 VBG denotes employing
T43523 12564-12565 DT denotes a
T43525 12566-12575 VBN denotes motorized
T43526 12575-12577 , denotes ,
T43527 12577-12582 NN denotes speed
T43528 12583-12593 VBN denotes controlled
T43529 12593-12595 , denotes ,
T43530 12595-12602 JJ denotes modular
T43531 12603-12612 NN denotes treadmill
T43524 12613-12619 NN denotes system
T43532 12620-12621 -LRB- denotes (
T43534 12621-12629 NNP denotes Columbus
T43533 12630-12641 NNP denotes Instruments
T43535 12641-12642 -RRB- denotes )
T43536 12642-12643 . denotes .
T43537 12643-12739 sentence denotes The treadmill was equipped with an electric shock stimulus and an adjustable inclination angle.
T43538 12644-12647 DT denotes The
T43539 12648-12657 NN denotes treadmill
T43541 12658-12661 VBD denotes was
T43540 12662-12670 VBN denotes equipped
T43542 12671-12675 IN denotes with
T43543 12676-12678 DT denotes an
T43545 12679-12687 JJ denotes electric
T43546 12688-12693 NN denotes shock
T43544 12694-12702 NN denotes stimulus
T43547 12703-12706 CC denotes and
T43548 12707-12709 DT denotes an
T43550 12710-12720 JJ denotes adjustable
T43551 12721-12732 NN denotes inclination
T43549 12733-12738 NN denotes angle
T43552 12738-12739 . denotes .
T43553 12739-12809 sentence denotes Running velocity was set at 35 m/min, with a level inclination angle.
T43554 12740-12747 NN denotes Running
T43555 12748-12756 NN denotes velocity
T43557 12757-12760 VBD denotes was
T43556 12761-12764 VBN denotes set
T43558 12765-12767 IN denotes at
T43559 12768-12770 CD denotes 35
T43560 12771-12772 NN denotes m
T43561 12772-12773 SYM denotes /
T43562 12773-12776 NN denotes min
T43563 12776-12778 , denotes ,
T43564 12778-12782 IN denotes with
T43565 12783-12784 DT denotes a
T43567 12785-12790 NN denotes level
T43568 12791-12802 NN denotes inclination
T43566 12803-12808 NN denotes angle
T43569 12808-12809 . denotes .
T44022 12811-12817 NN denotes VO2max
T44023 12818-12825 NNS denotes studies
T44024 12825-12954 sentence denotes VO2max was determined while the mice were running on a treadmill using an open flow system (Columbus Instruments Oxymax System).
T44025 12826-12832 NN denotes VO2max
T44027 12833-12836 VBD denotes was
T44026 12837-12847 VBN denotes determined
T44028 12848-12853 IN denotes while
T44030 12854-12857 DT denotes the
T44031 12858-12862 NNS denotes mice
T44032 12863-12867 VBD denotes were
T44029 12868-12875 VBG denotes running
T44033 12876-12878 IN denotes on
T44034 12879-12880 DT denotes a
T44035 12881-12890 NN denotes treadmill
T44036 12891-12896 VBG denotes using
T44037 12897-12899 DT denotes an
T44039 12900-12904 JJ denotes open
T44040 12905-12909 NN denotes flow
T44038 12910-12916 NN denotes system
T44041 12917-12918 -LRB- denotes (
T44043 12918-12926 NNP denotes Columbus
T44044 12927-12938 NNP denotes Instruments
T44045 12939-12945 NNP denotes Oxymax
T44042 12946-12952 NNP denotes System
T44046 12952-12953 -RRB- denotes )
T44047 12953-12954 . denotes .
T44048 12954-13056 sentence denotes All measurements of oxygen consumption took place at an elevation of 150 m (ambient PBAR = 745 torr).
T44049 12955-12958 DT denotes All
T44050 12959-12971 NNS denotes measurements
T44052 12972-12974 IN denotes of
T44053 12975-12981 NN denotes oxygen
T44054 12982-12993 NN denotes consumption
T44051 12994-12998 VBD denotes took
T44055 12999-13004 NN denotes place
T44056 13005-13007 IN denotes at
T44057 13008-13010 DT denotes an
T44058 13011-13020 NN denotes elevation
T44059 13021-13023 IN denotes of
T44060 13024-13027 CD denotes 150
T44061 13028-13029 NN denotes m
T44062 13030-13031 -LRB- denotes (
T44064 13031-13038 NN denotes ambient
T44065 13039-13043 NN denotes PBAR
T44066 13044-13045 SYM denotes =
T44067 13046-13049 CD denotes 745
T44063 13050-13054 NN denotes torr
T44068 13054-13055 -RRB- denotes )
T44069 13055-13056 . denotes .
T44070 13056-13151 sentence denotes Animals were placed into the metabolic chamber for 3–5 min to allow the system to equilibrate.
T44071 13057-13064 NNS denotes Animals
T44073 13065-13069 VBD denotes were
T44072 13070-13076 VBN denotes placed
T44074 13077-13081 IN denotes into
T44075 13082-13085 DT denotes the
T44077 13086-13095 JJ denotes metabolic
T44076 13096-13103 NN denotes chamber
T44078 13104-13107 IN denotes for
T44079 13108-13109 CD denotes 3
T44081 13109-13110 SYM denotes –
T44080 13110-13111 CD denotes 5
T44082 13112-13115 NN denotes min
T44083 13116-13118 TO denotes to
T44084 13119-13124 VB denotes allow
T44085 13125-13128 DT denotes the
T44086 13129-13135 NN denotes system
T44087 13136-13138 TO denotes to
T44088 13139-13150 VB denotes equilibrate
T44089 13150-13151 . denotes .
T44090 13151-13269 sentence denotes Mice were then induced to run up an 18° incline at a speed of 40 m/min using a shock grid in the rear of the chamber.
T44091 13152-13156 NNS denotes Mice
T44093 13157-13161 VBD denotes were
T44094 13162-13166 RB denotes then
T44092 13167-13174 VBN denotes induced
T44095 13175-13177 TO denotes to
T44096 13178-13181 VB denotes run
T44097 13182-13184 IN denotes up
T44098 13185-13187 DT denotes an
T44100 13188-13190 CD denotes 18
T44101 13190-13191 NN denotes °
T44099 13192-13199 NN denotes incline
T44102 13200-13202 IN denotes at
T44103 13203-13204 DT denotes a
T44104 13205-13210 NN denotes speed
T44105 13211-13213 IN denotes of
T44106 13214-13216 CD denotes 40
T44107 13217-13218 NN denotes m
T44108 13218-13219 SYM denotes /
T44109 13219-13222 NN denotes min
T44110 13223-13228 VBG denotes using
T44111 13229-13230 DT denotes a
T44113 13231-13236 NN denotes shock
T44112 13237-13241 NN denotes grid
T44114 13242-13244 IN denotes in
T44115 13245-13248 DT denotes the
T44116 13249-13253 NN denotes rear
T44117 13254-13256 IN denotes of
T44118 13257-13260 DT denotes the
T44119 13261-13268 NN denotes chamber
T44120 13268-13269 . denotes .
T44121 13269-13359 sentence denotes The speed was increased by 5 m/min every 2 min until the animals were unable to continue.
T44122 13270-13273 DT denotes The
T44123 13274-13279 NN denotes speed
T44125 13280-13283 VBD denotes was
T44124 13284-13293 VBN denotes increased
T44126 13294-13296 IN denotes by
T44127 13297-13298 CD denotes 5
T44128 13299-13300 NN denotes m
T44129 13300-13301 SYM denotes /
T44130 13301-13304 NN denotes min
T44131 13305-13310 DT denotes every
T44133 13311-13312 CD denotes 2
T44132 13313-13316 NN denotes min
T44134 13317-13322 IN denotes until
T44136 13323-13326 DT denotes the
T44137 13327-13334 NNS denotes animals
T44135 13335-13339 VBD denotes were
T44138 13340-13346 JJ denotes unable
T44139 13347-13349 TO denotes to
T44140 13350-13358 VB denotes continue
T44141 13358-13359 . denotes .
T44142 13359-13497 sentence denotes Maximal effort was determined when oxygen uptake did not increase with power output and subsequently the mouse failed to maintain effort.
T44143 13360-13367 JJ denotes Maximal
T44144 13368-13374 NN denotes effort
T44146 13375-13378 VBD denotes was
T44145 13379-13389 VBN denotes determined
T44147 13390-13394 WRB denotes when
T44149 13395-13401 NN denotes oxygen
T44150 13402-13408 NN denotes uptake
T44151 13409-13412 VBD denotes did
T44152 13413-13416 RB denotes not
T44148 13417-13425 VB denotes increase
T44153 13426-13430 IN denotes with
T44154 13431-13436 NN denotes power
T44155 13437-13443 NN denotes output
T44156 13444-13447 CC denotes and
T44157 13448-13460 RB denotes subsequently
T44159 13461-13464 DT denotes the
T44160 13465-13470 NN denotes mouse
T44158 13471-13477 VBD denotes failed
T44161 13478-13480 TO denotes to
T44162 13481-13489 VB denotes maintain
T44163 13490-13496 NN denotes effort
T44164 13496-13497 . denotes .
T44165 13497-13616 sentence denotes VO2max was calculated using the averaged values over 1 min during which the animal's O2 consumption reached a plateau.
T44166 13498-13504 NN denotes VO2max
T44168 13505-13508 VBD denotes was
T44167 13509-13519 VBN denotes calculated
T44169 13520-13525 VBG denotes using
T44170 13526-13529 DT denotes the
T44172 13530-13538 JJ denotes averaged
T44171 13539-13545 NNS denotes values
T44173 13546-13550 IN denotes over
T44174 13551-13552 CD denotes 1
T44175 13553-13556 NN denotes min
T44176 13557-13563 IN denotes during
T44178 13564-13569 WDT denotes which
T44179 13570-13573 DT denotes the
T44180 13574-13580 NN denotes animal
T44182 13580-13582 POS denotes 's
T44183 13583-13585 NN denotes O2
T44181 13586-13597 NN denotes consumption
T44177 13598-13605 VBD denotes reached
T44184 13606-13607 DT denotes a
T44185 13608-13615 NN denotes plateau
T44186 13615-13616 . denotes .
T44806 13618-13626 JJ denotes Isolated
T44808 13627-13633 NN denotes muscle
T44809 13634-13645 NN denotes stimulation
T44807 13646-13653 NNS denotes studies
T44810 13653-13750 sentence denotes Animals were anesthetized with ketamine/xylazine and the soleus muscle was removed from one leg.
T44811 13654-13661 NNS denotes Animals
T44813 13662-13666 VBD denotes were
T44812 13667-13679 VBN denotes anesthetized
T44814 13680-13684 IN denotes with
T44815 13685-13693 NN denotes ketamine
T44817 13693-13694 HYPH denotes /
T44816 13694-13702 NN denotes xylazine
T44818 13703-13706 CC denotes and
T44819 13707-13710 DT denotes the
T44821 13711-13717 NN denotes soleus
T44820 13718-13724 NN denotes muscle
T44823 13725-13728 VBD denotes was
T44822 13729-13736 VBN denotes removed
T44824 13737-13741 IN denotes from
T44825 13742-13745 CD denotes one
T44826 13746-13749 NN denotes leg
T44827 13749-13750 . denotes .
T44828 13750-13841 sentence denotes Upon removal, the muscle was suspended in a Krebs solution aerated with 95% O2 and 5% CO2.
T44829 13751-13755 IN denotes Upon
T44831 13756-13763 NN denotes removal
T44832 13763-13765 , denotes ,
T44833 13765-13768 DT denotes the
T44834 13769-13775 NN denotes muscle
T44835 13776-13779 VBD denotes was
T44830 13780-13789 VBN denotes suspended
T44836 13790-13792 IN denotes in
T44837 13793-13794 DT denotes a
T44839 13795-13800 NN denotes Krebs
T44838 13801-13809 NN denotes solution
T44840 13810-13817 VBN denotes aerated
T44841 13818-13822 IN denotes with
T44842 13823-13825 CD denotes 95
T44843 13825-13826 NN denotes %
T44844 13827-13829 NN denotes O2
T44845 13830-13833 CC denotes and
T44846 13834-13835 CD denotes 5
T44847 13835-13836 NN denotes %
T44848 13837-13840 NN denotes CO2
T44849 13840-13841 . denotes .
T44850 13841-14052 sentence denotes The muscle and Krebs solution were suspended within a water bath maintained at 37 °C, and the muscle was anchored to a Grass (West Warwick, Rhode Island, United States) isometric force transducer (model FTO3C).
T44851 13842-13845 DT denotes The
T44852 13846-13852 NN denotes muscle
T44854 13853-13856 CC denotes and
T44855 13857-13862 NN denotes Krebs
T44856 13863-13871 NN denotes solution
T44857 13872-13876 VBD denotes were
T44853 13877-13886 VBN denotes suspended
T44858 13887-13893 IN denotes within
T44859 13894-13895 DT denotes a
T44861 13896-13901 NN denotes water
T44860 13902-13906 NN denotes bath
T44862 13907-13917 VBN denotes maintained
T44863 13918-13920 IN denotes at
T44864 13921-13923 CD denotes 37
T44865 13924-13926 NN denotes °C
T44866 13926-13928 , denotes ,
T44867 13928-13931 CC denotes and
T44868 13932-13935 DT denotes the
T44869 13936-13942 NN denotes muscle
T44871 13943-13946 VBD denotes was
T44870 13947-13955 VBN denotes anchored
T44872 13956-13958 IN denotes to
T44873 13959-13960 DT denotes a
T44875 13961-13966 NNP denotes Grass
T44876 13967-13968 -LRB- denotes (
T44878 13968-13972 NNP denotes West
T44877 13973-13980 NNP denotes Warwick
T44879 13980-13982 , denotes ,
T44880 13982-13987 NNP denotes Rhode
T44881 13988-13994 NNP denotes Island
T44882 13994-13996 , denotes ,
T44883 13996-14002 NNP denotes United
T44884 14003-14009 NNP denotes States
T44885 14009-14010 -RRB- denotes )
T44886 14011-14020 JJ denotes isometric
T44887 14021-14026 NN denotes force
T44874 14027-14037 NN denotes transducer
T44888 14038-14039 -LRB- denotes (
T44890 14039-14044 NN denotes model
T44889 14045-14050 NN denotes FTO3C
T44891 14050-14051 -RRB- denotes )
T44892 14051-14052 . denotes .
T44893 14052-14207 sentence denotes Muscles were stimulated to contract with a Grass stimulator (model S88) generating a field stimulus through electrodes located at both ends of the muscle.
T44894 14053-14060 NNS denotes Muscles
T44896 14061-14065 VBD denotes were
T44895 14066-14076 VBN denotes stimulated
T44897 14077-14079 TO denotes to
T44898 14080-14088 VB denotes contract
T44899 14089-14093 IN denotes with
T44900 14094-14095 DT denotes a
T44902 14096-14101 NNP denotes Grass
T44901 14102-14112 NN denotes stimulator
T44903 14113-14114 -LRB- denotes (
T44905 14114-14119 NN denotes model
T44904 14120-14123 NN denotes S88
T44906 14123-14124 -RRB- denotes )
T44907 14125-14135 VBG denotes generating
T44908 14136-14137 DT denotes a
T44910 14138-14143 NN denotes field
T44909 14144-14152 NN denotes stimulus
T44911 14153-14160 IN denotes through
T44912 14161-14171 NNS denotes electrodes
T44913 14172-14179 VBN denotes located
T44914 14180-14182 IN denotes at
T44915 14183-14187 DT denotes both
T44916 14188-14192 NNS denotes ends
T44917 14193-14195 IN denotes of
T44918 14196-14199 DT denotes the
T44919 14200-14206 NN denotes muscle
T44920 14206-14207 . denotes .
T44921 14207-14330 sentence denotes Force-voltage (maximal force at about 100 V) and length-tension relationships were determined using single twitch stimuli.
T44922 14208-14213 NN denotes Force
T44924 14213-14214 HYPH denotes -
T44923 14214-14221 NN denotes voltage
T44926 14222-14223 -LRB- denotes (
T44927 14223-14230 JJ denotes maximal
T44928 14231-14236 NN denotes force
T44929 14237-14239 IN denotes at
T44930 14240-14245 JJ denotes about
T44931 14246-14249 CD denotes 100
T44932 14250-14251 NN denotes V
T44933 14251-14252 -RRB- denotes )
T44934 14253-14256 CC denotes and
T44935 14257-14263 NN denotes length
T44937 14263-14264 HYPH denotes -
T44936 14264-14271 NN denotes tension
T44925 14272-14285 NNS denotes relationships
T44939 14286-14290 VBD denotes were
T44938 14291-14301 VBN denotes determined
T44940 14302-14307 VBG denotes using
T44941 14308-14314 JJ denotes single
T44942 14315-14321 NN denotes twitch
T44943 14322-14329 NNS denotes stimuli
T44944 14329-14330 . denotes .
T44945 14330-14426 sentence denotes The stimulator then delivered repeating trains of stimuli at one per second at 40 Hz for 2 min.
T44946 14331-14334 DT denotes The
T44947 14335-14345 NN denotes stimulator
T44949 14346-14350 RB denotes then
T44948 14351-14360 VBD denotes delivered
T44950 14361-14370 VBG denotes repeating
T44951 14371-14377 NNS denotes trains
T44952 14378-14380 IN denotes of
T44953 14381-14388 NNS denotes stimuli
T44954 14389-14391 IN denotes at
T44955 14392-14395 CD denotes one
T44956 14396-14399 IN denotes per
T44957 14400-14406 NN denotes second
T44958 14407-14409 IN denotes at
T44959 14410-14412 CD denotes 40
T44960 14413-14415 NN denotes Hz
T44961 14416-14419 IN denotes for
T44962 14420-14421 CD denotes 2
T44963 14422-14425 NN denotes min
T44964 14425-14426 . denotes .
T44965 14426-14554 sentence denotes Each train lasted 330 ms, and were digitally recorded using MacLab (AD Instruments, Colorado Springs, Colorado, United States).
T44966 14427-14431 DT denotes Each
T44967 14432-14437 NN denotes train
T44968 14438-14444 VBD denotes lasted
T44969 14445-14448 CD denotes 330
T44970 14449-14451 NNS denotes ms
T44971 14451-14453 , denotes ,
T44972 14453-14456 CC denotes and
T44973 14457-14461 VBD denotes were
T44975 14462-14471 RB denotes digitally
T44974 14472-14480 VBN denotes recorded
T44976 14481-14486 VBG denotes using
T44977 14487-14493 NNP denotes MacLab
T44978 14494-14495 -LRB- denotes (
T44980 14495-14497 NNP denotes AD
T44979 14498-14509 NNPS denotes Instruments
T44981 14509-14511 , denotes ,
T44982 14511-14519 NNP denotes Colorado
T44983 14520-14527 NNP denotes Springs
T44984 14527-14529 , denotes ,
T44985 14529-14537 NNP denotes Colorado
T44986 14537-14539 , denotes ,
T44987 14539-14545 NNP denotes United
T44988 14546-14552 NNP denotes States
T44989 14552-14553 -RRB- denotes )
T44990 14553-14554 . denotes .
T44991 14554-14783 sentence denotes Fatigue resistance was calculated as the ratio of the force generated by the last tetanus divided by the highest force generated multiplied by 100 to give the percent of force generation that remained after the fatigue protocol.
T44992 14555-14562 NN denotes Fatigue
T44993 14563-14573 NN denotes resistance
T44995 14574-14577 VBD denotes was
T44994 14578-14588 VBN denotes calculated
T44996 14589-14591 IN denotes as
T44997 14592-14595 DT denotes the
T44998 14596-14601 NN denotes ratio
T44999 14602-14604 IN denotes of
T45000 14605-14608 DT denotes the
T45001 14609-14614 NN denotes force
T45002 14615-14624 VBN denotes generated
T45003 14625-14627 IN denotes by
T45004 14628-14631 DT denotes the
T45006 14632-14636 JJ denotes last
T45005 14637-14644 NN denotes tetanus
T45007 14645-14652 VBN denotes divided
T45008 14653-14655 IN denotes by
T45009 14656-14659 DT denotes the
T45011 14660-14667 JJS denotes highest
T45010 14668-14673 NN denotes force
T45012 14674-14683 VBN denotes generated
T45013 14684-14694 VBN denotes multiplied
T45014 14695-14697 IN denotes by
T45015 14698-14701 CD denotes 100
T45016 14702-14704 TO denotes to
T45017 14705-14709 VB denotes give
T45018 14710-14713 DT denotes the
T45019 14714-14721 NN denotes percent
T45020 14722-14724 IN denotes of
T45021 14725-14730 NN denotes force
T45022 14731-14741 NN denotes generation
T45023 14742-14746 WDT denotes that
T45024 14747-14755 VBD denotes remained
T45025 14756-14761 IN denotes after
T45026 14762-14765 DT denotes the
T45028 14766-14773 NN denotes fatigue
T45027 14774-14782 NN denotes protocol
T45029 14782-14783 . denotes .
T45215 14785-14793 NN denotes Exercise
T45216 14794-14810 NN denotes echocardiography
T45217 14810-14988 sentence denotes Adult female mice (6–8 mo old) were exercised on the motorized treadmill using the run-to-exhaustion settings described above for 60 s or until exhaustion, whichever came first.
T45218 14811-14816 JJ denotes Adult
T45220 14817-14823 JJ denotes female
T45219 14824-14828 NNS denotes mice
T45222 14829-14830 -LRB- denotes (
T45223 14830-14831 CD denotes 6
T45225 14831-14832 SYM denotes –
T45224 14832-14833 CD denotes 8
T45226 14834-14836 NN denotes mo
T45227 14837-14840 JJ denotes old
T45228 14840-14841 -RRB- denotes )
T45229 14842-14846 VBD denotes were
T45221 14847-14856 VBN denotes exercised
T45230 14857-14859 IN denotes on
T45231 14860-14863 DT denotes the
T45233 14864-14873 VBN denotes motorized
T45232 14874-14883 NN denotes treadmill
T45234 14884-14889 VBG denotes using
T45235 14890-14893 DT denotes the
T45237 14894-14897 VB denotes run
T45238 14897-14898 HYPH denotes -
T45239 14898-14900 IN denotes to
T45240 14900-14901 HYPH denotes -
T45241 14901-14911 NN denotes exhaustion
T45236 14912-14920 NNS denotes settings
T45242 14921-14930 VBN denotes described
T45243 14931-14936 RB denotes above
T45244 14937-14940 IN denotes for
T45245 14941-14943 CD denotes 60
T45246 14944-14945 NN denotes s
T45247 14946-14948 CC denotes or
T45248 14949-14954 IN denotes until
T45249 14955-14965 NN denotes exhaustion
T45250 14965-14967 , denotes ,
T45252 14967-14976 WDT denotes whichever
T45251 14977-14981 VBD denotes came
T45253 14982-14987 RB denotes first
T45254 14987-14988 . denotes .
T45255 14988-15168 sentence denotes Immediately following its treadmill run, the mouse was subjected to serial echocardiography using an Acuson Sequoia Echocardiography System performed as previously described [51].
T45256 14989-15000 RB denotes Immediately
T45257 15001-15010 IN denotes following
T45259 15011-15014 PRP$ denotes its
T45261 15015-15024 NN denotes treadmill
T45260 15025-15028 NN denotes run
T45262 15028-15030 , denotes ,
T45263 15030-15033 DT denotes the
T45264 15034-15039 NN denotes mouse
T45265 15040-15043 VBD denotes was
T45258 15044-15053 VBN denotes subjected
T45266 15054-15056 IN denotes to
T45267 15057-15063 JJ denotes serial
T45268 15064-15080 NN denotes echocardiography
T45269 15081-15086 VBG denotes using
T45270 15087-15089 DT denotes an
T45272 15090-15096 NNP denotes Acuson
T45274 15097-15104 NNP denotes Sequoia
T45273 15105-15121 NNP denotes Echocardiography
T45271 15122-15128 NNP denotes System
T45275 15129-15138 VBN denotes performed
T45276 15139-15141 IN denotes as
T45278 15142-15152 RB denotes previously
T45277 15153-15162 VBN denotes described
T45279 15163-15164 -LRB- denotes [
T45280 15164-15166 CD denotes 51
T45281 15166-15167 -RRB- denotes ]
T45282 15167-15168 . denotes .
T45734 15170-15172 FW denotes In
T45735 15173-15177 FW denotes vivo
T45737 15178-15185 JJ denotes cardiac
T45738 15186-15197 JJ denotes hemodynamic
T45736 15198-15205 NNS denotes studies
T45739 15205-15294 sentence denotes Hemodynamic studies were performed as previously described with some modifications [52].
T45740 15206-15217 JJ denotes Hemodynamic
T45741 15218-15225 NNS denotes studies
T45743 15226-15230 VBD denotes were
T45742 15231-15240 VBN denotes performed
T45744 15241-15243 IN denotes as
T45746 15244-15254 RB denotes previously
T45745 15255-15264 VBN denotes described
T45747 15265-15269 IN denotes with
T45748 15270-15274 DT denotes some
T45749 15275-15288 NNS denotes modifications
T45750 15289-15290 -LRB- denotes [
T45751 15290-15292 CD denotes 52
T45752 15292-15293 -RRB- denotes ]
T45753 15293-15294 . denotes .
T45754 15294-15402 sentence denotes In brief, adult mice (10–12 wk) were anesthetized intraperitoneally (IP) with thiopental sodium (60 mg/kg).
T45755 15295-15297 IN denotes In
T45757 15298-15303 JJ denotes brief
T45758 15303-15305 , denotes ,
T45759 15305-15310 JJ denotes adult
T45760 15311-15315 NNS denotes mice
T45761 15316-15317 -LRB- denotes (
T45763 15317-15319 CD denotes 10
T45765 15319-15320 SYM denotes –
T45764 15320-15322 CD denotes 12
T45762 15323-15325 NN denotes wk
T45766 15325-15326 -RRB- denotes )
T45767 15327-15331 VBD denotes were
T45756 15332-15344 VBN denotes anesthetized
T45768 15345-15362 RB denotes intraperitoneally
T45769 15363-15364 -LRB- denotes (
T45770 15364-15366 RB denotes IP
T45771 15366-15367 -RRB- denotes )
T45772 15368-15372 IN denotes with
T45773 15373-15383 NN denotes thiopental
T45774 15384-15390 NN denotes sodium
T45775 15391-15392 -LRB- denotes (
T45777 15392-15394 CD denotes 60
T45776 15395-15397 NN denotes mg
T45778 15397-15398 SYM denotes /
T45779 15398-15400 NN denotes kg
T45780 15400-15401 -RRB- denotes )
T45781 15401-15402 . denotes .
T45782 15402-15468 sentence denotes The mice were intubated and ventilated with a Harvard ventilator.
T45783 15403-15406 DT denotes The
T45784 15407-15411 NNS denotes mice
T45786 15412-15416 VBD denotes were
T45785 15417-15426 VBN denotes intubated
T45787 15427-15430 CC denotes and
T45788 15431-15441 VBN denotes ventilated
T45789 15442-15446 IN denotes with
T45790 15447-15448 DT denotes a
T45792 15449-15456 NNP denotes Harvard
T45791 15457-15467 NN denotes ventilator
T45793 15467-15468 . denotes .
T45794 15468-15738 sentence denotes The right carotid artery was isolated in the region of the trachea and cannulated with a 1.4-French high-fidelity micromanometer catheter (Millar Instruments, Houston, Texas, United States), which was inserted into the left ventricle retrograde across the aortic valve.
T45795 15469-15472 DT denotes The
T45797 15473-15478 JJ denotes right
T45798 15479-15486 NN denotes carotid
T45796 15487-15493 NN denotes artery
T45800 15494-15497 VBD denotes was
T45799 15498-15506 VBN denotes isolated
T45801 15507-15509 IN denotes in
T45802 15510-15513 DT denotes the
T45803 15514-15520 NN denotes region
T45804 15521-15523 IN denotes of
T45805 15524-15527 DT denotes the
T45806 15528-15535 NN denotes trachea
T45807 15536-15539 CC denotes and
T45808 15540-15550 VBN denotes cannulated
T45809 15551-15555 IN denotes with
T45810 15556-15557 DT denotes a
T45812 15558-15561 CD denotes 1.4
T45814 15561-15562 HYPH denotes -
T45813 15562-15568 NNP denotes French
T45815 15569-15573 JJ denotes high
T45817 15573-15574 HYPH denotes -
T45816 15574-15582 NN denotes fidelity
T45818 15583-15597 NN denotes micromanometer
T45811 15598-15606 NN denotes catheter
T45819 15607-15608 -LRB- denotes (
T45821 15608-15614 NNP denotes Millar
T45820 15615-15626 NNP denotes Instruments
T45822 15626-15628 , denotes ,
T45823 15628-15635 NNP denotes Houston
T45824 15635-15637 , denotes ,
T45825 15637-15642 NNP denotes Texas
T45826 15642-15644 , denotes ,
T45827 15644-15650 NNP denotes United
T45828 15651-15657 NNP denotes States
T45829 15657-15658 -RRB- denotes )
T45830 15658-15660 , denotes ,
T45831 15660-15665 WDT denotes which
T45833 15666-15669 VBD denotes was
T45832 15670-15678 VBN denotes inserted
T45834 15679-15683 IN denotes into
T45835 15684-15687 DT denotes the
T45837 15688-15692 JJ denotes left
T45836 15693-15702 NN denotes ventricle
T45838 15703-15713 RB denotes retrograde
T45839 15714-15720 IN denotes across
T45840 15721-15724 DT denotes the
T45842 15725-15731 JJ denotes aortic
T45841 15732-15737 NN denotes valve
T45843 15737-15738 . denotes .
T45844 15738-15933 sentence denotes Hemodynamic measurements were recorded at baseline and 3 min following continuous infusion of incremental doses of dobutamine (β1, β2, and α1-adrenergic agonist) up to 32 ng · gBW−1· min−1 [53].
T45845 15739-15750 JJ denotes Hemodynamic
T45846 15751-15763 NNS denotes measurements
T45848 15764-15768 VBD denotes were
T45847 15769-15777 VBN denotes recorded
T45849 15778-15780 IN denotes at
T45850 15781-15789 NN denotes baseline
T45851 15790-15793 CC denotes and
T45852 15794-15795 CD denotes 3
T45853 15796-15799 NN denotes min
T45854 15800-15809 IN denotes following
T45855 15810-15820 JJ denotes continuous
T45856 15821-15829 NN denotes infusion
T45857 15830-15832 IN denotes of
T45858 15833-15844 JJ denotes incremental
T45859 15845-15850 NNS denotes doses
T45860 15851-15853 IN denotes of
T45861 15854-15864 NN denotes dobutamine
T45862 15865-15866 -LRB- denotes (
T45863 15866-15868 NN denotes β1
T45865 15868-15870 , denotes ,
T45866 15870-15872 NN denotes β2
T45867 15872-15874 , denotes ,
T45868 15874-15877 CC denotes and
T45869 15878-15880 NN denotes α1
T45870 15880-15881 HYPH denotes -
T45864 15881-15891 JJ denotes adrenergic
T45871 15892-15899 NN denotes agonist
T45872 15899-15900 -RRB- denotes )
T45873 15901-15903 IN denotes up
T45874 15904-15906 IN denotes to
T45875 15907-15909 CD denotes 32
T45876 15910-15912 NN denotes ng
T45877 15913-15914 SYM denotes ·
T45878 15915-15918 NN denotes gBW
T45879 15918-15919 SYM denotes −
T45880 15919-15920 CD denotes 1
T45881 15920-15921 SYM denotes ·
T45882 15922-15925 NN denotes min
T45883 15925-15926 SYM denotes −
T45884 15926-15927 CD denotes 1
T45885 15928-15929 -LRB- denotes [
T45886 15929-15931 CD denotes 53
T45887 15931-15932 -RRB- denotes ]
T45888 15932-15933 . denotes .
T45889 15933-16107 sentence denotes Continuous pressure-volume data were acquired and digitized with the BioBench computer software data acquisition system (National Instruments, Austin, Texas, United States).
T45890 15934-15944 JJ denotes Continuous
T45892 15945-15953 NN denotes pressure
T45894 15953-15954 HYPH denotes -
T45893 15954-15960 NN denotes volume
T45891 15961-15965 NNS denotes data
T45896 15966-15970 VBD denotes were
T45895 15971-15979 VBN denotes acquired
T45897 15980-15983 CC denotes and
T45898 15984-15993 VBN denotes digitized
T45899 15994-15998 IN denotes with
T45900 15999-16002 DT denotes the
T45902 16003-16011 NNP denotes BioBench
T45904 16012-16020 NN denotes computer
T45903 16021-16029 NN denotes software
T45905 16030-16034 NNS denotes data
T45906 16035-16046 NN denotes acquisition
T45901 16047-16053 NN denotes system
T45907 16054-16055 -LRB- denotes (
T45909 16055-16063 NNP denotes National
T45908 16064-16075 NNPS denotes Instruments
T45910 16075-16077 , denotes ,
T45911 16077-16083 NNP denotes Austin
T45912 16083-16085 , denotes ,
T45913 16085-16090 NNP denotes Texas
T45914 16090-16092 , denotes ,
T45915 16092-16098 NNP denotes United
T45916 16099-16105 NNP denotes States
T45917 16105-16106 -RRB- denotes )
T45918 16106-16107 . denotes .
T46753 16109-16117 VBN denotes Isolated
T46755 16118-16125 VBG denotes working
T46756 16126-16131 NN denotes mouse
T46757 16132-16137 NN denotes heart
T46754 16138-16147 NN denotes perfusion
T46758 16147-16238 sentence denotes Isolated working mouse heart perfusion was based on a previously described procedure [54].
T46759 16148-16156 VBN denotes Isolated
T46761 16157-16164 VBG denotes working
T46762 16165-16170 NN denotes mouse
T46763 16171-16176 NN denotes heart
T46760 16177-16186 NN denotes perfusion
T46765 16187-16190 VBD denotes was
T46764 16191-16196 VBN denotes based
T46766 16197-16199 IN denotes on
T46767 16200-16201 DT denotes a
T46769 16202-16212 RB denotes previously
T46770 16213-16222 VBN denotes described
T46768 16223-16232 NN denotes procedure
T46771 16233-16234 -LRB- denotes [
T46772 16234-16236 CD denotes 54
T46773 16236-16237 -RRB- denotes ]
T46774 16237-16238 . denotes .
T46775 16238-16322 sentence denotes Adult mice (4–7 mo old) were heparinized (100 units IP) 10 min prior to anesthesia.
T46776 16239-16244 JJ denotes Adult
T46777 16245-16249 NNS denotes mice
T46779 16250-16251 -LRB- denotes (
T46781 16251-16252 CD denotes 4
T46783 16252-16253 SYM denotes –
T46782 16253-16254 CD denotes 7
T46784 16255-16257 NN denotes mo
T46780 16258-16261 JJ denotes old
T46785 16261-16262 -RRB- denotes )
T46786 16263-16267 VBD denotes were
T46778 16268-16279 VBN denotes heparinized
T46787 16280-16281 -LRB- denotes (
T46789 16281-16284 CD denotes 100
T46788 16285-16290 NNS denotes units
T46790 16291-16293 RB denotes IP
T46791 16293-16294 -RRB- denotes )
T46792 16295-16297 CD denotes 10
T46793 16298-16301 NN denotes min
T46795 16302-16307 JJ denotes prior
T46794 16308-16310 IN denotes to
T46796 16311-16321 NN denotes anesthesia
T46797 16321-16322 . denotes .
T46798 16322-16401 sentence denotes Animals were then deeply anesthetized with 5–10 mg of sodium pentobarbital IP.
T46799 16323-16330 NNS denotes Animals
T46801 16331-16335 VBD denotes were
T46802 16336-16340 RB denotes then
T46803 16341-16347 RB denotes deeply
T46800 16348-16360 VBN denotes anesthetized
T46804 16361-16365 IN denotes with
T46805 16366-16367 CD denotes 5
T46807 16367-16368 SYM denotes –
T46806 16368-16370 CD denotes 10
T46808 16371-16373 NN denotes mg
T46809 16374-16376 IN denotes of
T46810 16377-16383 NN denotes sodium
T46811 16384-16397 NN denotes pentobarbital
T46812 16398-16400 RB denotes IP
T46813 16400-16401 . denotes .
T46814 16401-16610 sentence denotes Hearts were excised and placed in an ice-cold Krebs-Henseleit bicarbonate (KHB) solution [118 mM NaCl, 25 mM NaHCO3, 4.7 mM KCl, 1.2 mM KH2PO4, 2.5 mM CaCl2, 5.0 mM glucose, and 100 units/L insulin (pH 7.4)].
T46815 16402-16408 NNS denotes Hearts
T46817 16409-16413 VBD denotes were
T46816 16414-16421 VBN denotes excised
T46818 16422-16425 CC denotes and
T46819 16426-16432 VBN denotes placed
T46820 16433-16435 IN denotes in
T46821 16436-16438 DT denotes an
T46823 16439-16442 NN denotes ice
T46825 16442-16443 HYPH denotes -
T46824 16443-16447 JJ denotes cold
T46826 16448-16453 NN denotes Krebs
T46828 16453-16454 HYPH denotes -
T46827 16454-16463 NN denotes Henseleit
T46829 16464-16475 NN denotes bicarbonate
T46830 16476-16477 -LRB- denotes (
T46831 16477-16480 NN denotes KHB
T46832 16480-16481 -RRB- denotes )
T46822 16482-16490 NN denotes solution
T46833 16491-16492 -LRB- denotes [
T46834 16492-16495 CD denotes 118
T46835 16496-16498 NN denotes mM
T46836 16499-16503 NN denotes NaCl
T46837 16503-16505 , denotes ,
T46838 16505-16507 CD denotes 25
T46839 16508-16510 NN denotes mM
T46840 16511-16517 NN denotes NaHCO3
T46841 16517-16519 , denotes ,
T46842 16519-16522 CD denotes 4.7
T46843 16523-16525 NN denotes mM
T46844 16526-16529 NN denotes KCl
T46845 16529-16531 , denotes ,
T46846 16531-16534 CD denotes 1.2
T46847 16535-16537 NN denotes mM
T46848 16538-16544 NN denotes KH2PO4
T46849 16544-16546 , denotes ,
T46850 16546-16549 CD denotes 2.5
T46851 16550-16552 NN denotes mM
T46852 16553-16558 NN denotes CaCl2
T46853 16558-16560 , denotes ,
T46854 16560-16563 CD denotes 5.0
T46855 16564-16566 NN denotes mM
T46856 16567-16574 NN denotes glucose
T46857 16574-16576 , denotes ,
T46858 16576-16579 CC denotes and
T46859 16580-16583 CD denotes 100
T46860 16584-16589 NNS denotes units
T46861 16589-16590 SYM denotes /
T46863 16590-16591 NN denotes L
T46862 16592-16599 NN denotes insulin
T46864 16600-16601 -LRB- denotes (
T46865 16601-16603 NN denotes pH
T46866 16604-16607 CD denotes 7.4
T46867 16607-16608 -RRB- denotes )
T46868 16608-16609 -RRB- denotes ]
T46869 16609-16610 . denotes .
T46870 16610-16706 sentence denotes Hearts were cannulated first via the aorta and perfused retrogradely by the Langendorff method.
T46871 16611-16617 NNS denotes Hearts
T46873 16618-16622 VBD denotes were
T46872 16623-16633 VBN denotes cannulated
T46874 16634-16639 RB denotes first
T46875 16640-16643 IN denotes via
T46876 16644-16647 DT denotes the
T46877 16648-16653 NN denotes aorta
T46878 16654-16657 CC denotes and
T46879 16658-16666 VBN denotes perfused
T46880 16667-16679 RB denotes retrogradely
T46881 16680-16682 IN denotes by
T46882 16683-16686 DT denotes the
T46884 16687-16698 NNP denotes Langendorff
T46883 16699-16705 NN denotes method
T46885 16705-16706 . denotes .
T46886 16706-16982 sentence denotes Following left atrial cannulation, perfusion was switched to the working mode with KHB solution containing 1.2 mM palmitate bound to 3% fatty acid-free BSA with a preload pressure of 11.5 mm Hg and an afterload pressure of 50 mm Hg for 60 min with oxygenated buffer solution.
T46887 16707-16716 VBG denotes Following
T46889 16717-16721 JJ denotes left
T46891 16722-16728 JJ denotes atrial
T46890 16729-16740 NN denotes cannulation
T46892 16740-16742 , denotes ,
T46893 16742-16751 NN denotes perfusion
T46894 16752-16755 VBD denotes was
T46888 16756-16764 VBN denotes switched
T46895 16765-16767 IN denotes to
T46896 16768-16771 DT denotes the
T46898 16772-16779 VBG denotes working
T46897 16780-16784 NN denotes mode
T46899 16785-16789 IN denotes with
T46901 16790-16793 NN denotes KHB
T46902 16794-16802 NN denotes solution
T46903 16803-16813 VBG denotes containing
T46904 16814-16817 CD denotes 1.2
T46905 16818-16820 NN denotes mM
T46906 16821-16830 NN denotes palmitate
T46900 16831-16836 VBN denotes bound
T46907 16837-16839 IN denotes to
T46908 16840-16841 CD denotes 3
T46909 16841-16842 NN denotes %
T46911 16843-16848 JJ denotes fatty
T46912 16849-16853 NN denotes acid
T46914 16853-16854 HYPH denotes -
T46913 16854-16858 JJ denotes free
T46910 16859-16862 NN denotes BSA
T46915 16863-16867 IN denotes with
T46916 16868-16869 DT denotes a
T46918 16870-16877 JJ denotes preload
T46917 16878-16886 NN denotes pressure
T46919 16887-16889 IN denotes of
T46920 16890-16894 CD denotes 11.5
T46921 16895-16897 NN denotes mm
T46922 16898-16900 NN denotes Hg
T46923 16901-16904 CC denotes and
T46924 16905-16907 DT denotes an
T46926 16908-16917 JJ denotes afterload
T46925 16918-16926 NN denotes pressure
T46927 16927-16929 IN denotes of
T46928 16930-16932 CD denotes 50
T46929 16933-16935 NN denotes mm
T46930 16936-16938 NN denotes Hg
T46931 16939-16942 IN denotes for
T46932 16943-16945 CD denotes 60
T46933 16946-16949 NN denotes min
T46934 16950-16954 IN denotes with
T46935 16955-16965 VBN denotes oxygenated
T46937 16966-16972 NN denotes buffer
T46936 16973-16981 NN denotes solution
T46938 16981-16982 . denotes .
T46939 16982-17356 sentence denotes Functional measurements, namely cardiac output, aortic flows, peak systolic pressure, and heart rate were acquired every 10 min using inline flow probes (Transonic Systems, Ithaca, New York, United States), a pressure transducer (TSD 104A, BIOPAC Systems, Santa Barbara, California, United States) and data acquired with the MP100 system from AcqKnowledge (BIOPAC Systems).
T46940 16983-16993 JJ denotes Functional
T46941 16994-17006 NNS denotes measurements
T46943 17006-17008 , denotes ,
T46944 17008-17014 RB denotes namely
T46946 17015-17022 JJ denotes cardiac
T46945 17023-17029 NN denotes output
T46947 17029-17031 , denotes ,
T46948 17031-17037 JJ denotes aortic
T46949 17038-17043 NNS denotes flows
T46950 17043-17045 , denotes ,
T46951 17045-17049 JJ denotes peak
T46953 17050-17058 JJ denotes systolic
T46952 17059-17067 NN denotes pressure
T46954 17067-17069 , denotes ,
T46955 17069-17072 CC denotes and
T46956 17073-17078 NN denotes heart
T46957 17079-17083 NN denotes rate
T46958 17084-17088 VBD denotes were
T46942 17089-17097 VBN denotes acquired
T46959 17098-17103 DT denotes every
T46961 17104-17106 CD denotes 10
T46960 17107-17110 NN denotes min
T46962 17111-17116 VBG denotes using
T46963 17117-17123 NN denotes inline
T46965 17124-17128 NN denotes flow
T46964 17129-17135 NNS denotes probes
T46966 17136-17137 -LRB- denotes (
T46968 17137-17146 NNP denotes Transonic
T46967 17147-17154 NNP denotes Systems
T46969 17154-17156 , denotes ,
T46970 17156-17162 NNP denotes Ithaca
T46971 17162-17164 , denotes ,
T46972 17164-17167 NNP denotes New
T46973 17168-17172 NNP denotes York
T46974 17172-17174 , denotes ,
T46975 17174-17180 NNP denotes United
T46976 17181-17187 NNP denotes States
T46977 17187-17188 -RRB- denotes )
T46978 17188-17190 , denotes ,
T46979 17190-17191 DT denotes a
T46981 17192-17200 NN denotes pressure
T46980 17201-17211 NN denotes transducer
T46982 17212-17213 -LRB- denotes (
T46984 17213-17216 NN denotes TSD
T46983 17217-17221 NN denotes 104A
T46985 17221-17223 , denotes ,
T46986 17223-17229 NNP denotes BIOPAC
T46987 17230-17237 NNP denotes Systems
T46988 17237-17239 , denotes ,
T46989 17239-17244 NNP denotes Santa
T46990 17245-17252 NNP denotes Barbara
T46991 17252-17254 , denotes ,
T46992 17254-17264 NNP denotes California
T46993 17264-17266 , denotes ,
T46994 17266-17272 NNP denotes United
T46995 17273-17279 NNP denotes States
T46996 17279-17280 -RRB- denotes )
T46997 17281-17284 CC denotes and
T46998 17285-17289 NNS denotes data
T46999 17290-17298 VBN denotes acquired
T47000 17299-17303 IN denotes with
T47001 17304-17307 DT denotes the
T47003 17308-17313 NN denotes MP100
T47002 17314-17320 NN denotes system
T47004 17321-17325 IN denotes from
T47005 17326-17338 NNP denotes AcqKnowledge
T47006 17339-17340 -LRB- denotes (
T47008 17340-17346 NNP denotes BIOPAC
T47007 17347-17354 NNP denotes Systems
T47009 17354-17355 -RRB- denotes )
T47010 17355-17356 . denotes .
T47011 17356-17445 sentence denotes Cardiac work was calculated as the product of peak systolic pressure and cardiac output.
T47012 17357-17364 JJ denotes Cardiac
T47013 17365-17369 NN denotes work
T47015 17370-17373 VBD denotes was
T47014 17374-17384 VBN denotes calculated
T47016 17385-17387 IN denotes as
T47017 17388-17391 DT denotes the
T47018 17392-17399 NN denotes product
T47019 17400-17402 IN denotes of
T47020 17403-17407 NN denotes peak
T47022 17408-17416 JJ denotes systolic
T47021 17417-17425 NN denotes pressure
T47023 17426-17429 CC denotes and
T47024 17430-17437 JJ denotes cardiac
T47025 17438-17444 NN denotes output
T47026 17444-17445 . denotes .
T47482 17447-17457 NNS denotes Statistics
T47483 17457-17557 sentence denotes Data were analyzed using T-tests or ANOVAs (measures of general activity and sensorimotor battery).
T47484 17458-17462 NNS denotes Data
T47486 17463-17467 VBD denotes were
T47485 17468-17476 VBN denotes analyzed
T47487 17477-17482 VBG denotes using
T47488 17483-17484 NN denotes T
T47490 17484-17485 HYPH denotes -
T47489 17485-17490 NNS denotes tests
T47491 17491-17493 CC denotes or
T47492 17494-17500 NNS denotes ANOVAs
T47493 17501-17502 -LRB- denotes (
T47494 17502-17510 NNS denotes measures
T47495 17511-17513 IN denotes of
T47496 17514-17521 JJ denotes general
T47497 17522-17530 NN denotes activity
T47498 17531-17534 CC denotes and
T47499 17535-17547 NN denotes sensorimotor
T47500 17548-17555 NN denotes battery
T47501 17555-17556 -RRB- denotes )
T47502 17556-17557 . denotes .
T47503 17557-17617 sentence denotes The level of significance was set at p < 0.05 in all cases.
T47504 17558-17561 DT denotes The
T47505 17562-17567 NN denotes level
T47507 17568-17570 IN denotes of
T47508 17571-17583 NN denotes significance
T47509 17584-17587 VBD denotes was
T47506 17588-17591 VBN denotes set
T47510 17592-17594 IN denotes at
T47511 17595-17596 NN denotes p
T47512 17597-17598 SYM denotes <
T47513 17599-17603 CD denotes 0.05
T47514 17604-17606 IN denotes in
T47515 17607-17610 DT denotes all
T47516 17611-17616 NNS denotes cases
T47517 17616-17617 . denotes .
T47518 17617-17681 sentence denotes Data are reported as mean values ± SEM, unless otherwise noted.
T47519 17618-17622 NNS denotes Data
T47521 17623-17626 VBP denotes are
T47520 17627-17635 VBN denotes reported
T47522 17636-17638 IN denotes as
T47523 17639-17643 NN denotes mean
T47524 17644-17650 NNS denotes values
T47525 17651-17652 SYM denotes ±
T47526 17653-17656 NN denotes SEM
T47527 17656-17658 , denotes ,
T47528 17658-17664 IN denotes unless
T47530 17665-17674 RB denotes otherwise
T47529 17675-17680 VBN denotes noted
T47531 17680-17681 . denotes .
T47532 17681-17830 sentence denotes The ANOVA model used to analyze each sensorimotor test included one between-subjects variable (genotype), and one within-subjects variable (trials).
T47533 17682-17685 DT denotes The
T47535 17686-17691 NN denotes ANOVA
T47534 17692-17697 NN denotes model
T47537 17698-17702 VBN denotes used
T47538 17703-17705 TO denotes to
T47539 17706-17713 VB denotes analyze
T47540 17714-17718 DT denotes each
T47542 17719-17731 NN denotes sensorimotor
T47541 17732-17736 NN denotes test
T47536 17737-17745 VBD denotes included
T47543 17746-17749 CD denotes one
T47544 17750-17757 IN denotes between
T47545 17757-17758 HYPH denotes -
T47546 17758-17766 NNS denotes subjects
T47547 17767-17775 JJ denotes variable
T47548 17776-17777 -LRB- denotes (
T47549 17777-17785 NN denotes genotype
T47550 17785-17786 -RRB- denotes )
T47551 17786-17788 , denotes ,
T47552 17788-17791 CC denotes and
T47553 17792-17795 CD denotes one
T47554 17796-17802 IN denotes within
T47555 17802-17803 HYPH denotes -
T47556 17803-17811 NNS denotes subjects
T47557 17812-17820 JJ denotes variable
T47558 17821-17822 -LRB- denotes (
T47559 17822-17828 NNS denotes trials
T47560 17828-17829 -RRB- denotes )
T47561 17829-17830 . denotes .
T47562 17830-18120 sentence denotes When ANOVAs with repeated measures were conducted, the Huynh-Feldt (H-F) adjustment of alpha levels was used for all within-subjects effects containing more than two levels, in order to protect against violations of the sphericity/compound symmetry assumptions underlying this ANOVA model.
T47563 17831-17835 WRB denotes When
T47565 17836-17842 NNS denotes ANOVAs
T47566 17843-17847 IN denotes with
T47567 17848-17856 VBN denotes repeated
T47568 17857-17865 NNS denotes measures
T47569 17866-17870 VBD denotes were
T47564 17871-17880 VBN denotes conducted
T47571 17880-17882 , denotes ,
T47572 17882-17885 DT denotes the
T47574 17886-17891 NNP denotes Huynh
T47576 17891-17892 HYPH denotes -
T47575 17892-17897 NNP denotes Feldt
T47577 17898-17899 -LRB- denotes (
T47578 17899-17900 NN denotes H
T47580 17900-17901 HYPH denotes -
T47579 17901-17902 NN denotes F
T47581 17902-17903 -RRB- denotes )
T47573 17904-17914 NN denotes adjustment
T47582 17915-17917 IN denotes of
T47583 17918-17923 NN denotes alpha
T47584 17924-17930 NNS denotes levels
T47585 17931-17934 VBD denotes was
T47570 17935-17939 VBN denotes used
T47586 17940-17943 IN denotes for
T47587 17944-17947 DT denotes all
T47589 17948-17954 IN denotes within
T47590 17954-17955 HYPH denotes -
T47591 17955-17963 NNS denotes subjects
T47588 17964-17971 NNS denotes effects
T47592 17972-17982 VBG denotes containing
T47593 17983-17987 JJR denotes more
T47595 17988-17992 IN denotes than
T47594 17993-17996 CD denotes two
T47596 17997-18003 NNS denotes levels
T47597 18003-18005 , denotes ,
T47598 18005-18007 IN denotes in
T47599 18008-18013 NN denotes order
T47600 18014-18016 TO denotes to
T47601 18017-18024 VB denotes protect
T47602 18025-18032 IN denotes against
T47603 18033-18043 NNS denotes violations
T47604 18044-18046 IN denotes of
T47605 18047-18050 DT denotes the
T47607 18051-18061 NN denotes sphericity
T47608 18061-18062 HYPH denotes /
T47609 18062-18070 JJ denotes compound
T47610 18071-18079 NN denotes symmetry
T47606 18080-18091 NNS denotes assumptions
T47611 18092-18102 VBG denotes underlying
T47612 18103-18107 DT denotes this
T47614 18108-18113 NN denotes ANOVA
T47613 18114-18119 NN denotes model
T47615 18119-18120 . denotes .
T47616 18120-18281 sentence denotes In addition, Bonferroni correction was used when appropriate to help maintain prescribed alpha levels (e.g., p < 0.05) when multiple comparisons were conducted.
T47617 18121-18123 IN denotes In
T47619 18124-18132 NN denotes addition
T47620 18132-18134 , denotes ,
T47621 18134-18144 NNP denotes Bonferroni
T47622 18145-18155 NN denotes correction
T47623 18156-18159 VBD denotes was
T47618 18160-18164 VBN denotes used
T47624 18165-18169 WRB denotes when
T47625 18170-18181 JJ denotes appropriate
T47626 18182-18184 TO denotes to
T47627 18185-18189 VB denotes help
T47628 18190-18198 VB denotes maintain
T47629 18199-18209 VBN denotes prescribed
T47631 18210-18215 NN denotes alpha
T47630 18216-18222 NNS denotes levels
T47632 18223-18224 -LRB- denotes (
T47634 18224-18228 FW denotes e.g.
T47635 18228-18230 , denotes ,
T47633 18230-18231 NN denotes p
T47636 18232-18233 SYM denotes <
T47637 18234-18238 CD denotes 0.05
T47638 18238-18239 -RRB- denotes )
T47639 18240-18244 WRB denotes when
T47641 18245-18253 JJ denotes multiple
T47642 18254-18265 NNS denotes comparisons
T47643 18266-18270 VBD denotes were
T47640 18271-18280 VBN denotes conducted
T47644 18280-18281 . denotes .
R10000 T38960 T38961 punct [,35
R10001 T38961 T38959 parataxis 35,described
R10002 T38962 T38961 punct ],35
R10003 T38963 T38956 punct .,performed
R10004 T38965 T38966 amod Prior,to
R10005 T38966 T38967 prep to,fasted
R10006 T38968 T38966 pobj studies,to
R10007 T38969 T38967 punct ", ",fasted
R10008 T38970 T38967 nsubjpass mice,fasted
R10009 T38971 T38967 auxpass were,fasted
R10010 T38972 T38973 advmod overnight,h
R10011 T38973 T38967 npadvmod h,fasted
R10012 T38974 T38975 punct (,GTT
R10013 T38975 T38972 parataxis GTT,overnight
R10014 T38976 T38975 punct ),GTT
R10015 T38977 T38973 cc or,h
R10016 T38978 T38973 nummod 6,h
R10017 T38979 T38980 punct (,ITT
R10018 T38980 T38973 parataxis ITT,h
R10019 T38981 T38980 punct ),ITT
R10020 T38982 T38967 punct .,fasted
R10021 T38984 T38985 prep In,injected
R10022 T38986 T38987 compound GTT,studies
R10023 T38987 T38984 pobj studies,In
R10024 T38988 T38985 punct ", ",injected
R10025 T38989 T38985 nsubjpass mice,injected
R10026 T38990 T38985 auxpass were,injected
R10027 T38991 T38985 prep with,injected
R10028 T38992 T38993 det a,solution
R10029 T38993 T38991 pobj solution,with
R10030 T38994 T38995 nummod 10,%
R10031 T38995 T38993 compound %,solution
R10032 T38996 T38993 prep of,solution
R10033 T38997 T38998 compound D,glucose
R10034 T38998 T38996 pobj glucose,of
R10035 T38999 T38998 punct -,glucose
R10036 T39000 T39001 punct (,g
R10037 T39001 T38998 parataxis g,glucose
R10038 T39002 T39001 nummod 1,g
R10039 T39003 T39004 punct /,kg
R10040 T39004 T39001 prep kg,g
R10041 T39005 T39001 punct ),g
R10042 T39006 T38985 punct .,injected
R10043 T39008 T39009 prep For,received
R10044 T39010 T39008 pobj ITT,For
R10045 T39011 T39009 punct ", ",received
R10046 T39012 T39009 nsubj mice,received
R10047 T39013 T39014 det an,injection
R10048 T39014 T39009 dobj injection,received
R10049 T39015 T39014 compound IP,injection
R10050 T39016 T39014 prep of,injection
R10051 T39017 T39018 amod human,insulin
R10052 T39018 T39016 pobj insulin,of
R10053 T39019 T39018 amod regular,insulin
R10054 T39020 T39021 punct (,Lilly
R10055 T39021 T39014 parataxis Lilly,injection
R10056 T39022 T39021 compound Eli,Lilly
R10057 T39023 T39021 punct ", ",Lilly
R10058 T39024 T39021 npadvmod Indianapolis,Lilly
R10059 T39025 T39021 punct ", ",Lilly
R10060 T39026 T39021 npadvmod Indiana,Lilly
R10061 T39027 T39021 punct ", ",Lilly
R10062 T39028 T39029 compound United,States
R10063 T39029 T39021 npadvmod States,Lilly
R10064 T39030 T39021 punct ),Lilly
R10065 T39031 T39009 prep at,received
R10066 T39032 T39033 det a,dose
R10067 T39033 T39031 pobj dose,at
R10068 T39034 T39033 prep of,dose
R10069 T39035 T39036 nummod 0.75,units
R10070 T39036 T39034 pobj units,of
R10071 T39037 T39038 punct /,kg
R10072 T39038 T39036 prep kg,units
R10073 T39039 T39038 prep of,kg
R10074 T39040 T39041 compound body,weight
R10075 T39041 T39039 pobj weight,of
R10076 T39042 T39009 punct .,received
R10077 T39044 T39045 compound Tail,glucose
R10078 T39045 T39047 nsubjpass glucose,determined
R10079 T39046 T39045 compound blood,glucose
R10080 T39048 T39047 auxpass was,determined
R10081 T39049 T39047 prep at,determined
R10082 T39050 T39051 nummod 0,min
R10083 T39051 T39049 pobj min,at
R10084 T39052 T39050 punct ", ",0
R10085 T39053 T39050 conj 30,0
R10086 T39054 T39053 punct ", ",30
R10087 T39055 T39053 conj 60,30
R10088 T39056 T39055 punct ", ",60
R10089 T39057 T39055 cc and,60
R10090 T39058 T39055 conj 120,60
R10091 T39059 T39051 prep after,min
R10092 T39060 T39059 pobj challenge,after
R10093 T39061 T39047 advcl using,determined
R10094 T39062 T39063 det a,Analyzer
R10095 T39063 T39061 dobj Analyzer,using
R10096 T39064 T39065 compound B,GLUCOSE
R10097 T39065 T39063 compound GLUCOSE,Analyzer
R10098 T39066 T39065 punct -,GLUCOSE
R10099 T39067 T39068 punct (,AB
R10100 T39068 T39061 parataxis AB,using
R10101 T39069 T39068 compound Hemacue,AB
R10102 T39070 T39068 punct ", ",AB
R10103 T39071 T39068 npadvmod Angelholm,AB
R10104 T39072 T39068 punct ", ",AB
R10105 T39073 T39068 npadvmod Sweden,AB
R10106 T39074 T39068 punct ),AB
R10107 T39075 T39047 punct .,determined
R10108 T39319 T39320 amod Indirect,calorimetry
R10109 T39322 T39323 compound Oxygen,rates
R10110 T39323 T39325 nsubjpass rates,measured
R10111 T39324 T39323 compound consumption,rates
R10112 T39326 T39323 punct (,rates
R10113 T39327 T39323 appos VO2,rates
R10114 T39328 T39323 punct ),rates
R10115 T39329 T39323 prep of,rates
R10116 T39330 T39331 nummod 5,wk
R10117 T39331 T39333 npadvmod wk,old
R10118 T39332 T39331 punct -,wk
R10119 T39333 T39335 amod old,mice
R10120 T39334 T39333 punct -,old
R10121 T39335 T39329 pobj mice,of
R10122 T39336 T39335 amod female,mice
R10123 T39337 T39325 auxpass were,measured
R10124 T39338 T39325 advcl using,measured
R10125 T39339 T39340 det a,System
R10126 T39340 T39338 dobj System,using
R10127 T39341 T39342 compound Columbus,Instruments
R10128 T39342 T39340 compound Instruments,System
R10129 T39343 T39340 compound Oxymax,System
R10130 T39344 T39325 punct .,measured
R10131 T39346 T39347 compound Resting,rates
R10132 T39347 T39351 nsubjpass rates,assessed
R10133 T39348 T39347 compound baseline,rates
R10134 T39349 T39350 compound oxygen,consumption
R10135 T39350 T39347 compound consumption,rates
R10136 T39352 T39351 auxpass were,assessed
R10137 T39353 T39351 prep for,assessed
R10138 T39354 T39355 advmod at,least
R10139 T39355 T39356 advmod least,1.0
R10140 T39356 T39357 nummod 1.0,h
R10141 T39357 T39353 pobj h,for
R10142 T39358 T39351 punct .,assessed
R10143 T39360 T39361 prep For,dissolved
R10144 T39362 T39363 npadvmod β3,adrenergic
R10145 T39363 T39365 amod adrenergic,studies
R10146 T39364 T39363 punct -,adrenergic
R10147 T39365 T39360 pobj studies,For
R10148 T39366 T39365 compound stimulation,studies
R10149 T39367 T39361 punct ", ",dissolved
R10150 T39368 T39361 nsubjpass BRL,dissolved
R10151 T39369 T39368 nummod 37344,BRL
R10152 T39370 T39371 punct (,Sigma
R10153 T39371 T39368 parataxis Sigma,BRL
R10154 T39372 T39371 punct ),Sigma
R10155 T39373 T39361 auxpass was,dissolved
R10156 T39374 T39361 prep in,dissolved
R10157 T39375 T39376 amod sterile,saline
R10158 T39376 T39374 pobj saline,in
R10159 T39377 T39361 cc and,dissolved
R10160 T39378 T39361 conj injected,dissolved
R10161 T39379 T39378 advmod IP,injected
R10162 T39380 T39381 punct (,μg
R10163 T39381 T39378 parataxis μg,injected
R10164 T39382 T39381 nummod 2,μg
R10165 T39383 T39384 punct /,g
R10166 T39384 T39381 prep g,μg
R10167 T39385 T39384 prep of,g
R10168 T39386 T39387 compound body,weight
R10169 T39387 T39385 pobj weight,of
R10170 T39388 T39381 punct ),μg
R10171 T39389 T39390 punct [,45
R10172 T39390 T39378 parataxis 45,injected
R10173 T39391 T39390 punct ],45
R10174 T39392 T39361 punct .,dissolved
R10175 T39394 T39395 compound Postagonist,assessment
R10176 T39395 T39396 nsubjpass assessment,recorded
R10177 T39397 T39395 prep of,assessment
R10178 T39398 T39399 compound oxygen,consumption
R10179 T39399 T39397 pobj consumption,of
R10180 T39400 T39396 auxpass was,recorded
R10181 T39401 T39396 prep for,recorded
R10182 T39402 T39403 det an,h
R10183 T39403 T39401 pobj h,for
R10184 T39404 T39403 amod additional,h
R10185 T39405 T39403 nummod 1.0,h
R10186 T39406 T39396 punct ", ",recorded
R10187 T39407 T39396 prep with,recorded
R10188 T39408 T39407 pobj data,with
R10189 T39409 T39408 acl collected,data
R10190 T39410 T39409 prep at,collected
R10191 T39411 T39412 det the,point
R10192 T39412 T39410 pobj point,at
R10193 T39413 T39414 nummod 40,min
R10194 T39414 T39412 compound min,point
R10195 T39415 T39414 punct -,min
R10196 T39416 T39412 compound time,point
R10197 T39417 T39396 punct .,recorded
R10200 T40047 T40046 cc and,Histology
R10201 T40048 T40049 compound electron,microscopy
R10202 T40049 T40046 conj microscopy,Histology
R10203 T40051 T40052 compound Soleus,muscle
R10204 T40052 T40053 nsubjpass muscle,dissected
R10205 T40054 T40052 cc and,muscle
R10206 T40055 T40052 conj liver,muscle
R10207 T40056 T40053 auxpass were,dissected
R10208 T40057 T40053 cc and,dissected
R10209 T40058 T40053 conj fixed,dissected
R10210 T40059 T40058 advmod overnight,fixed
R10211 T40060 T40058 prep in,fixed
R10212 T40061 T40062 nummod 2,%
R10213 T40062 T40063 nmod %,glutaraldehyde
R10214 T40063 T40064 nmod glutaraldehyde,buffer
R10215 T40064 T40060 pobj buffer,in
R10216 T40065 T40063 punct ", ",glutaraldehyde
R10217 T40066 T40067 nummod 1,%
R10218 T40067 T40068 compound %,paraformaldehyde
R10219 T40068 T40063 conj paraformaldehyde,glutaraldehyde
R10220 T40069 T40068 punct ", ",paraformaldehyde
R10221 T40070 T40068 cc and,paraformaldehyde
R10222 T40071 T40072 nummod 0.08,%
R10223 T40072 T40068 conj %,paraformaldehyde
R10224 T40073 T40074 compound sodium,cacodylate
R10225 T40074 T40072 appos cacodylate,%
R10226 T40075 T40053 punct .,dissected
R10227 T40077 T40078 det The,tissues
R10228 T40078 T40079 nsubjpass tissues,postfixed
R10229 T40080 T40079 auxpass were,postfixed
R10230 T40081 T40079 prep in,postfixed
R10231 T40082 T40083 nummod 1,%
R10232 T40083 T40084 compound %,tetroxide
R10233 T40084 T40081 pobj tetroxide,in
R10234 T40085 T40084 compound osmium,tetroxide
R10235 T40086 T40079 punct ", ",postfixed
R10236 T40087 T40079 conj dehydrated,postfixed
R10237 T40088 T40087 prep in,dehydrated
R10238 T40089 T40090 amod graded,ethanol
R10239 T40090 T40088 pobj ethanol,in
R10240 T40091 T40087 punct ", ",dehydrated
R10241 T40092 T40087 conj embedded,dehydrated
R10242 T40093 T40092 prep in,embedded
R10243 T40094 T40095 compound Poly,Bed
R10244 T40095 T40096 compound Bed,resin
R10245 T40096 T40093 pobj resin,in
R10246 T40097 T40096 compound plastic,resin
R10247 T40098 T40092 punct ", ",embedded
R10248 T40099 T40092 cc and,embedded
R10249 T40100 T40092 conj sectioned,embedded
R10250 T40101 T40100 prep for,sectioned
R10251 T40102 T40103 compound electron,microscopy
R10252 T40103 T40101 pobj microscopy,for
R10253 T40104 T40079 punct .,postfixed
R10254 T40106 T40107 amod Cardiac,muscle
R10255 T40107 T40110 nmod muscle,densities
R10256 T40108 T40106 cc and,Cardiac
R10257 T40109 T40106 conj skeletal,Cardiac
R10258 T40110 T40115 nsubjpass densities,determined
R10259 T40111 T40110 amod mitochondrial,densities
R10260 T40112 T40111 cc and,mitochondrial
R10261 T40113 T40111 conj myofibrillar,mitochondrial
R10262 T40114 T40110 compound volume,densities
R10263 T40116 T40115 auxpass were,determined
R10264 T40117 T40115 prep from,determined
R10265 T40118 T40119 compound electron,micrographs
R10266 T40119 T40117 pobj micrographs,from
R10267 T40120 T40121 mark as,described
R10268 T40121 T40115 advcl described,determined
R10269 T40122 T40121 advmod previously,described
R10270 T40123 T40124 punct [,10
R10271 T40124 T40121 parataxis 10,described
R10272 T40125 T40124 punct ],10
R10273 T40126 T40115 punct .,determined
R10274 T40128 T40129 prep For,quantified
R10275 T40130 T40131 det each,animal
R10276 T40131 T40128 pobj animal,For
R10277 T40132 T40129 punct ", ",quantified
R10278 T40133 T40134 nummod three,fields
R10279 T40134 T40129 nsubjpass fields,quantified
R10280 T40135 T40134 amod different,fields
R10281 T40136 T40134 prep at,fields
R10282 T40137 T40138 det the,magnification
R10283 T40138 T40136 pobj magnification,at
R10284 T40139 T40138 prep of,magnification
R10285 T40140 T40139 pobj 7500,of
R10286 T40141 T40140 punct ×,7500
R10287 T40142 T40129 auxpass were,quantified
R10288 T40143 T40129 prep in,quantified
R10289 T40144 T40145 amod blinded,fashion
R10290 T40145 T40143 pobj fashion,in
R10291 T40146 T40129 punct .,quantified
R10292 T40148 T40149 nsubjpass Data,expressed
R10293 T40150 T40149 auxpass were,expressed
R10294 T40151 T40149 prep as,expressed
R10295 T40152 T40153 compound mean,density
R10296 T40153 T40151 pobj density,as
R10297 T40154 T40153 compound volume,density
R10298 T40155 T40153 prep of,density
R10299 T40156 T40155 pobj mitochondria,of
R10300 T40157 T40156 cc or,mitochondria
R10301 T40158 T40156 conj myofibrils,mitochondria
R10302 T40159 T40153 prep in,density
R10303 T40160 T40161 det each,field
R10304 T40161 T40159 pobj field,in
R10305 T40162 T40149 punct .,expressed
R10306 T40164 T40165 prep For,prepared
R10307 T40166 T40167 nmod electron,analysis
R10308 T40167 T40164 pobj analysis,For
R10309 T40168 T40167 amod microscopic,analysis
R10310 T40169 T40167 prep of,analysis
R10311 T40170 T40171 det the,brain
R10312 T40171 T40169 pobj brain,of
R10313 T40172 T40165 punct ", ",prepared
R10314 T40173 T40165 nsubjpass tissue,prepared
R10315 T40174 T40165 auxpass was,prepared
R10316 T40175 T40176 mark as,described
R10317 T40176 T40165 advcl described,prepared
R10318 T40177 T40176 advmod previously,described
R10319 T40178 T40179 punct [,46
R10320 T40179 T40176 parataxis 46,described
R10321 T40180 T40179 punct ],46
R10322 T40181 T40165 punct .,prepared
R10323 T40183 T40184 amod Ultrathin,sections
R10324 T40184 T40185 nsubjpass sections,cut
R10325 T40186 T40184 prep of,sections
R10326 T40187 T40186 pobj cortex,of
R10327 T40188 T40185 auxpass were,cut
R10328 T40189 T40185 prep onto,cut
R10329 T40190 T40191 npadvmod formvar,coated
R10330 T40191 T40193 amod coated,grids
R10331 T40192 T40191 punct -,coated
R10332 T40193 T40189 pobj grids,onto
R10333 T40194 T40193 compound slot,grids
R10334 T40195 T40193 acl stained,grids
R10335 T40196 T40195 prep with,stained
R10336 T40197 T40198 compound uranyl,acetate
R10337 T40198 T40196 pobj acetate,with
R10338 T40199 T40198 cc and,acetate
R10339 T40200 T40201 compound lead,citrate
R10340 T40201 T40198 conj citrate,acetate
R10341 T40202 T40185 cc and,cut
R10342 T40203 T40185 conj examined,cut
R10343 T40204 T40203 prep with,examined
R10344 T40205 T40206 det a,microscope
R10345 T40206 T40204 pobj microscope,with
R10346 T40207 T40206 nmod JEOL,microscope
R10347 T40208 T40207 nummod 1200,JEOL
R10348 T40209 T40206 compound electron,microscope
R10349 T40210 T40185 punct .,cut
R10350 T40212 T40213 prep For,dehydrated
R10351 T40214 T40215 nmod H,staining
R10352 T40215 T40212 pobj staining,For
R10353 T40216 T40214 cc &,H
R10354 T40217 T40214 conj E,H
R10355 T40218 T40213 punct ", ",dehydrated
R10356 T40219 T40213 nsubjpass sections,dehydrated
R10357 T40220 T40219 prep of,sections
R10358 T40221 T40220 pobj brain,of
R10359 T40222 T40219 punct ", ",sections
R10360 T40223 T40219 prep including,sections
R10361 T40224 T40225 amod cerebral,cortex
R10362 T40225 T40223 pobj cortex,including
R10363 T40226 T40225 punct ", ",cortex
R10364 T40227 T40225 conj brainstem,cortex
R10365 T40228 T40227 punct ", ",brainstem
R10366 T40229 T40227 cc and,brainstem
R10367 T40230 T40227 conj cerebellum,brainstem
R10368 T40231 T40213 punct ", ",dehydrated
R10369 T40232 T40213 auxpass were,dehydrated
R10370 T40233 T40213 prep in,dehydrated
R10371 T40234 T40235 amod graded,concentrations
R10372 T40235 T40233 pobj concentrations,in
R10373 T40236 T40235 prep of,concentrations
R10374 T40237 T40236 pobj alcohol,of
R10375 T40238 T40213 cc and,dehydrated
R10376 T40239 T40213 conj embedded,dehydrated
R10377 T40240 T40239 prep in,embedded
R10378 T40241 T40240 pobj paraffin,in
R10379 T40242 T40243 prep from,prepared
R10380 T40243 T40241 relcl prepared,paraffin
R10381 T40244 T40242 pobj which,from
R10382 T40245 T40246 nummod 5,μm
R10383 T40246 T40248 compound μm,sections
R10384 T40247 T40246 punct -,μm
R10385 T40248 T40243 nsubjpass sections,prepared
R10386 T40249 T40243 auxpass were,prepared
R10387 T40250 T40213 punct .,dehydrated
R10388 T40760 T40761 amod Primary,studies
R10389 T40762 T40763 compound mouse,hepatocyte
R10390 T40763 T40761 compound hepatocyte,studies
R10391 T40765 T40766 amod Primary,cultures
R10392 T40766 T40767 nsubjpass cultures,prepared
R10393 T40768 T40766 prep of,cultures
R10394 T40769 T40770 compound mouse,hepatocytes
R10395 T40770 T40768 pobj hepatocytes,of
R10396 T40771 T40767 auxpass were,prepared
R10397 T40772 T40767 prep from,prepared
R10398 T40773 T40774 amod male,mice
R10399 T40774 T40772 pobj mice,from
R10400 T40775 T40776 nmod PGC,1α
R10401 T40776 T40774 nmod 1α,mice
R10402 T40777 T40776 punct -,1α
R10403 T40778 T40776 punct +,1α
R10404 T40779 T40776 punct /,1α
R10405 T40780 T40776 punct +,1α
R10406 T40781 T40776 cc and,1α
R10407 T40782 T40783 compound PGC,1α
R10408 T40783 T40776 conj 1α,1α
R10409 T40784 T40783 punct -,1α
R10410 T40785 T40783 punct −,1α
R10411 T40786 T40783 punct /,1α
R10412 T40787 T40783 punct −,1α
R10413 T40788 T40789 advmod essentially,described
R10414 T40789 T40767 advcl described,prepared
R10415 T40790 T40789 mark as,described
R10416 T40791 T40792 punct [,47
R10417 T40792 T40789 parataxis 47,described
R10418 T40793 T40792 punct ],47
R10419 T40794 T40767 punct .,prepared
R10420 T40796 T40797 amod Fatty,acid
R10421 T40797 T40798 nmod acid,oxidation
R10422 T40798 T40799 nmod oxidation,experiments
R10423 T40799 T40803 nsubjpass experiments,commenced
R10424 T40800 T40798 cc and,oxidation
R10425 T40801 T40802 compound triglyceride,synthesis
R10426 T40802 T40798 conj synthesis,oxidation
R10427 T40804 T40803 auxpass were,commenced
R10428 T40805 T40806 quantmod 2,3
R10429 T40806 T40808 nummod 3,h
R10430 T40807 T40806 punct –,3
R10431 T40808 T40809 npadvmod h,plated
R10432 T40809 T40803 advcl plated,commenced
R10433 T40810 T40809 mark after,plated
R10434 T40811 T40812 det the,cells
R10435 T40812 T40809 nsubjpass cells,plated
R10436 T40813 T40809 auxpass were,plated
R10437 T40814 T40803 punct .,commenced
R10438 T40816 T40817 compound Triglyceride,synthesis
R10439 T40817 T40818 compound synthesis,studies
R10440 T40818 T40819 nsubjpass studies,performed
R10441 T40820 T40819 punct ", ",performed
R10442 T40821 T40819 auxpass were,performed
R10443 T40822 T40823 mark as,described
R10444 T40823 T40819 advcl described,performed
R10445 T40824 T40823 advmod previously,described
R10446 T40825 T40826 punct [,47
R10447 T40826 T40823 parataxis 47,described
R10448 T40827 T40826 punct ],47
R10449 T40828 T40819 punct .,performed
R10450 T40830 T40831 compound Palmitate,rates
R10451 T40831 T40833 nsubjpass rates,quantified
R10452 T40832 T40831 compound oxidation,rates
R10453 T40834 T40833 auxpass were,quantified
R10454 T40835 T40833 advcl using,quantified
R10455 T40836 T40835 punct [,using
R10456 T40837 T40838 nummod 9,3H
R10457 T40838 T40842 npadvmod 3H,palmitic
R10458 T40839 T40838 punct ",",3H
R10459 T40840 T40838 nummod 10,3H
R10460 T40841 T40838 punct -,3H
R10461 T40842 T40845 amod palmitic,acid
R10462 T40843 T40842 punct ],palmitic
R10463 T40844 T40842 punct -,palmitic
R10464 T40845 T40835 dobj acid,using
R10465 T40846 T40847 mark as,described
R10466 T40847 T40835 advcl described,using
R10467 T40848 T40849 punct [,48
R10468 T40849 T40847 parataxis 48,described
R10469 T40850 T40849 punct ],48
R10470 T40851 T40833 cc and,quantified
R10471 T40852 T40833 conj corrected,quantified
R10472 T40853 T40852 prep for,corrected
R10473 T40854 T40855 amod total,content
R10474 T40855 T40853 pobj content,for
R10475 T40856 T40855 amod cellular,content
R10476 T40857 T40855 compound protein,content
R10477 T40858 T40833 punct .,quantified
R10478 T40860 T40861 prep For,spun
R10479 T40862 T40863 compound respiration,studies
R10480 T40863 T40860 pobj studies,For
R10481 T40864 T40861 punct ", ",spun
R10482 T40865 T40861 nsubjpass cells,spun
R10483 T40866 T40861 auxpass were,spun
R10484 T40867 T40861 prt down,spun
R10485 T40868 T40869 amod prior,to
R10486 T40869 T40861 prep to,spun
R10487 T40870 T40869 pobj plating,to
R10488 T40871 T40861 cc and,spun
R10489 T40872 T40861 conj resuspended,spun
R10490 T40873 T40872 prep in,resuspended
R10491 T40874 T40875 det a,buffer
R10492 T40875 T40873 pobj buffer,in
R10493 T40876 T40875 compound permeabilization,buffer
R10494 T40877 T40875 punct (,buffer
R10495 T40878 T40875 acl described,buffer
R10496 T40879 T40878 advmod above,described
R10497 T40880 T40875 punct ),buffer
R10498 T40881 T40875 acl containing,buffer
R10499 T40882 T40883 nummod 50,μg
R10500 T40883 T40884 nmod μg,saponin
R10501 T40884 T40881 dobj saponin,containing
R10502 T40885 T40886 punct /,ml
R10503 T40886 T40883 prep ml,μg
R10504 T40887 T40861 punct .,spun
R10505 T40889 T40890 compound Respiration,studies
R10506 T40890 T40891 nsubjpass studies,performed
R10507 T40892 T40891 auxpass were,performed
R10508 T40893 T40891 prep in,performed
R10509 T40894 T40895 det the,presence
R10510 T40895 T40893 pobj presence,in
R10511 T40896 T40895 prep of,presence
R10512 T40897 T40898 nummod 5,mM
R10513 T40898 T40899 compound mM,succinate
R10514 T40899 T40896 pobj succinate,of
R10515 T40900 T40891 prep in,performed
R10516 T40901 T40902 det the,presence
R10517 T40902 T40900 pobj presence,in
R10518 T40903 T40902 prep of,presence
R10519 T40904 T40905 nummod 10,μM
R10520 T40905 T40906 compound μM,rotenone
R10521 T40906 T40903 pobj rotenone,of
R10522 T40907 T40891 punct .,performed
R10523 T40909 T40910 compound Respiration,rates
R10524 T40910 T40911 nsubjpass rates,expressed
R10525 T40912 T40911 auxpass were,expressed
R10526 T40913 T40911 prep as,expressed
R10527 T40914 T40915 compound nmol,O2
R10528 T40915 T40913 pobj O2,as
R10529 T40916 T40915 punct ·,O2
R10530 T40917 T40915 appos min,O2
R10531 T40918 T40919 punct −,1
R10532 T40919 T40917 nummod 1,min
R10533 T40920 T40915 punct ·,O2
R10534 T40921 T40915 appos mg,O2
R10535 T40922 T40921 prep of,mg
R10536 T40923 T40922 pobj protein,of
R10537 T40924 T40925 punct −,1
R10538 T40925 T40921 nummod 1,mg
R10539 T40926 T40911 punct .,expressed
R10540 T42847 T42846 prep of,Evaluation
R10541 T42848 T42849 compound locomotor,activity
R10542 T42849 T42847 pobj activity,of
R10543 T42850 T42849 punct ", ",activity
R10544 T42851 T42852 compound sensorimotor,capabilities
R10545 T42852 T42849 conj capabilities,activity
R10546 T42853 T42852 punct ", ",capabilities
R10547 T42854 T42852 cc and,capabilities
R10548 T42855 T42856 compound muscle,function
R10549 T42856 T42852 conj function,capabilities
R10550 T42858 T42859 aux To,evaluate
R10551 T42859 T42860 advcl evaluate,evaluated
R10552 T42861 T42862 amod general,levels
R10553 T42862 T42859 dobj levels,evaluate
R10554 T42863 T42862 compound activity,levels
R10555 T42864 T42862 cc and,levels
R10556 T42865 T42866 compound muscle,use
R10557 T42866 T42862 conj use,levels
R10558 T42867 T42860 punct ", ",evaluated
R10559 T42868 T42860 nsubjpass mice,evaluated
R10560 T42869 T42860 auxpass were,evaluated
R10561 T42870 T42860 prep over,evaluated
R10562 T42871 T42872 det a,period
R10563 T42872 T42870 pobj period,over
R10564 T42873 T42874 nummod 1,h
R10565 T42874 T42872 compound h,period
R10566 T42875 T42874 punct -,h
R10567 T42876 T42860 prep in,evaluated
R10568 T42877 T42878 nmod transparent,polystyrene
R10569 T42878 T42876 pobj polystyrene,in
R10570 T42879 T42878 punct (,polystyrene
R10571 T42880 T42881 nummod 47.6,cm
R10572 T42881 T42878 nmod cm,polystyrene
R10573 T42882 T42883 punct ×,cm
R10574 T42883 T42881 prep cm,cm
R10575 T42884 T42883 nummod 25.4,cm
R10576 T42885 T42886 punct ×,cm
R10577 T42886 T42881 prep cm,cm
R10578 T42887 T42886 nummod 20.6,cm
R10579 T42888 T42878 punct ),polystyrene
R10580 T42889 T42878 nmod enclosures,polystyrene
R10581 T42890 T42891 mark as,described
R10582 T42891 T42860 advcl described,evaluated
R10583 T42892 T42891 advmod previously,described
R10584 T42893 T42894 punct [,49
R10585 T42894 T42891 parataxis 49,described
R10586 T42895 T42894 punct ],49
R10587 T42896 T42860 advcl using,evaluated
R10588 T42897 T42898 det a,system
R10589 T42898 T42896 dobj system,using
R10590 T42899 T42900 amod high,resolution
R10591 T42900 T42898 compound resolution,system
R10592 T42901 T42900 punct -,resolution
R10593 T42902 T42898 compound photobeam,system
R10594 T42903 T42904 punct (,Monitor
R10595 T42904 T42898 parataxis Monitor,system
R10596 T42905 T42904 compound Motor,Monitor
R10597 T42906 T42904 punct ", ",Monitor
R10598 T42907 T42908 compound Hamilton,Kinder
R10599 T42908 T42904 npadvmod Kinder,Monitor
R10600 T42909 T42908 punct -,Kinder
R10601 T42910 T42904 punct ", ",Monitor
R10602 T42911 T42904 npadvmod Poway,Monitor
R10603 T42912 T42904 punct ", ",Monitor
R10604 T42913 T42904 npadvmod California,Monitor
R10605 T42914 T42904 punct ", ",Monitor
R10606 T42915 T42916 compound United,States
R10607 T42916 T42904 npadvmod States,Monitor
R10608 T42917 T42904 punct ),Monitor
R10609 T42918 T42860 punct .,evaluated
R10610 T42920 T42921 det Each,enclosure
R10611 T42921 T42922 nsubjpass enclosure,surrounded
R10612 T42923 T42922 auxpass was,surrounded
R10613 T42924 T42922 agent by,surrounded
R10614 T42925 T42926 det a,frame
R10615 T42926 T42924 pobj frame,by
R10616 T42927 T42926 acl containing,frame
R10617 T42928 T42929 det a,matrix
R10618 T42929 T42927 dobj matrix,containing
R10619 T42930 T42929 nummod 4,matrix
R10620 T42931 T42932 punct ×,8
R10621 T42932 T42930 prep 8,4
R10622 T42933 T42929 prep of,matrix
R10623 T42934 T42935 compound photocell,pairs
R10624 T42935 T42933 pobj pairs,of
R10625 T42936 T42929 punct ", ",matrix
R10626 T42937 T42938 det the,output
R10627 T42938 T42939 dep output,fed
R10628 T42939 T42929 relcl fed,matrix
R10629 T42940 T42938 prep of,output
R10630 T42941 T42940 pobj which,of
R10631 T42942 T42939 auxpass was,fed
R10632 T42943 T42939 prep to,fed
R10633 T42944 T42945 det an,computer
R10634 T42945 T42943 pobj computer,to
R10635 T42946 T42945 nmod on,computer
R10636 T42947 T42946 punct -,on
R10637 T42948 T42946 pobj line,on
R10638 T42949 T42922 punct .,surrounded
R10639 T42951 T42952 det The,software
R10640 T42952 T42954 nsubjpass software,used
R10641 T42953 T42952 compound system,software
R10642 T42955 T42956 punct (,Kinder
R10643 T42956 T42952 parataxis Kinder,software
R10644 T42957 T42956 compound Hamilton,Kinder
R10645 T42958 T42956 punct -,Kinder
R10646 T42959 T42956 punct ),Kinder
R10647 T42960 T42954 auxpass was,used
R10648 T42961 T42962 aux to,define
R10649 T42962 T42954 advcl define,used
R10650 T42963 T42964 det a,zone
R10651 T42964 T42962 dobj zone,define
R10652 T42965 T42966 nummod 33,cm
R10653 T42966 T42964 nmod cm,zone
R10654 T42967 T42968 punct ×,cm
R10655 T42968 T42966 prep cm,cm
R10656 T42969 T42968 nummod 11,cm
R10657 T42970 T42964 amod central,zone
R10658 T42971 T42964 cc and,zone
R10659 T42972 T42973 det a,zone
R10660 T42973 T42964 conj zone,zone
R10661 T42974 T42973 amod peripheral,zone
R10662 T42975 T42974 cc or,peripheral
R10663 T42976 T42974 conj surrounding,peripheral
R10664 T42977 T42978 dep that,was
R10665 T42978 T42973 relcl was,zone
R10666 T42979 T42980 nummod 5.5,cm
R10667 T42980 T42981 npadvmod cm,wide
R10668 T42981 T42978 acomp wide,was
R10669 T42982 T42978 prep with,was
R10670 T42983 T42984 det the,sides
R10671 T42984 T42985 nsubj sides,being
R10672 T42985 T42982 pcomp being,with
R10673 T42986 T42984 prep of,sides
R10674 T42987 T42988 det the,cage
R10675 T42988 T42986 pobj cage,of
R10676 T42989 T42990 det the,boundary
R10677 T42990 T42985 attr boundary,being
R10678 T42991 T42990 amod outermost,boundary
R10679 T42992 T42954 punct .,used
R10680 T42994 T42995 det This,area
R10681 T42995 T42997 nsubj area,extended
R10682 T42996 T42995 amod peripheral,area
R10683 T42998 T42997 prep along,extended
R10684 T42999 T43000 det the,perimeter
R10685 T43000 T42998 pobj perimeter,along
R10686 T43001 T43000 amod entire,perimeter
R10687 T43002 T43000 prep of,perimeter
R10688 T43003 T43004 det the,cage
R10689 T43004 T43002 pobj cage,of
R10690 T43005 T42997 punct .,extended
R10691 T43007 T43008 nsubj Variables,included
R10692 T43009 T43010 dep that,analyzed
R10693 T43010 T43007 relcl analyzed,Variables
R10694 T43011 T43010 auxpass were,analyzed
R10695 T43012 T43013 det the,number
R10696 T43013 T43008 dobj number,included
R10697 T43014 T43013 amod total,number
R10698 T43015 T43013 prep of,number
R10699 T43016 T43015 pobj ambulations,of
R10700 T43017 T43013 punct ", ",number
R10701 T43018 T43019 advmod as,as
R10702 T43019 T43013 cc as,number
R10703 T43020 T43019 advmod well,as
R10704 T43021 T43022 det the,number
R10705 T43022 T43013 conj number,number
R10706 T43023 T43022 prep of,number
R10707 T43024 T43023 pobj entries,of
R10708 T43025 T43022 punct ", ",number
R10709 T43026 T43027 det the,time
R10710 T43027 T43022 conj time,number
R10711 T43028 T43027 acl spent,time
R10712 T43029 T43027 punct ", ",time
R10713 T43030 T43027 cc and,time
R10714 T43031 T43032 det the,distance
R10715 T43032 T43027 conj distance,time
R10716 T43033 T43032 acl traveled,distance
R10717 T43034 T43033 prep in,traveled
R10718 T43035 T43036 det the,area
R10719 T43036 T43034 pobj area,in
R10720 T43037 T43036 compound center,area
R10721 T43038 T43039 advmod as,as
R10722 T43039 T43032 cc as,distance
R10723 T43040 T43039 advmod well,as
R10724 T43041 T43042 det the,distance
R10725 T43042 T43032 conj distance,distance
R10726 T43043 T43042 acl traveled,distance
R10727 T43044 T43043 prep in,traveled
R10728 T43045 T43046 det the,periphery
R10729 T43046 T43044 pobj periphery,in
R10730 T43047 T43046 acl surrounding,periphery
R10731 T43048 T43049 det the,center
R10732 T43049 T43047 dobj center,surrounding
R10733 T43050 T43008 punct .,included
R10734 T43052 T43053 det The,number
R10735 T43053 T43055 nsubjpass number,recorded
R10736 T43054 T43053 amod total,number
R10737 T43056 T43053 prep of,number
R10738 T43057 T43056 pobj ambulations,of
R10739 T43058 T43057 cc and,ambulations
R10740 T43059 T43057 conj rearings,ambulations
R10741 T43060 T43055 auxpass were,recorded
R10742 T43061 T43055 punct .,recorded
R10743 T43063 T43064 prep For,placed
R10744 T43065 T43066 det the,test
R10745 T43066 T43063 pobj test,For
R10746 T43067 T43066 amod inverted,test
R10747 T43068 T43066 compound screen,test
R10748 T43069 T43064 punct ", ",placed
R10749 T43070 T43064 nsubjpass mice,placed
R10750 T43071 T43064 auxpass were,placed
R10751 T43072 T43064 prep on,placed
R10752 T43073 T43074 det a,grid
R10753 T43074 T43072 pobj grid,on
R10754 T43075 T43074 compound wire,grid
R10755 T43076 T43074 compound mesh,grid
R10756 T43077 T43078 punct (,squares
R10757 T43078 T43074 parataxis squares,grid
R10758 T43079 T43078 nummod 16,squares
R10759 T43080 T43078 prep per,squares
R10760 T43081 T43082 nummod 10,cm
R10761 T43082 T43080 pobj cm,per
R10762 T43083 T43078 punct ),squares
R10763 T43084 T43064 cc and,placed
R10764 T43085 T43086 det the,screen
R10765 T43086 T43087 nsubjpass screen,inverted
R10766 T43087 T43064 conj inverted,placed
R10767 T43088 T43087 auxpass was,inverted
R10768 T43089 T43087 prep to,inverted
R10769 T43090 T43091 nummod 180,°
R10770 T43091 T43089 pobj °,to
R10771 T43092 T43064 punct .,placed
R10772 T43094 T43095 det A,score
R10773 T43095 T43097 nsubjpass score,given
R10774 T43096 T43095 amod maximum,score
R10775 T43098 T43095 prep of,score
R10776 T43099 T43100 nummod 60,s
R10777 T43100 T43098 pobj s,of
R10778 T43101 T43097 auxpass was,given
R10779 T43102 T43103 mark if,fall
R10780 T43103 T43097 advcl fall,given
R10781 T43104 T43105 det an,animal
R10782 T43105 T43103 nsubj animal,fall
R10783 T43106 T43103 aux did,fall
R10784 T43107 T43103 neg not,fall
R10785 T43108 T43097 punct .,given
R10786 T43110 T43111 det The,tests
R10787 T43111 T43112 nsubjpass tests,designed
R10788 T43113 T43111 acl included,tests
R10789 T43114 T43113 prep in,included
R10790 T43115 T43116 det the,battery
R10791 T43116 T43114 pobj battery,in
R10792 T43117 T43116 compound sensorimotor,battery
R10793 T43118 T43119 punct [,50
R10794 T43119 T43116 parataxis 50,battery
R10795 T43120 T43119 punct ],50
R10796 T43121 T43116 cc and,battery
R10797 T43122 T43123 amod accompanying,protocols
R10798 T43123 T43116 conj protocols,battery
R10799 T43124 T43112 auxpass were,designed
R10800 T43125 T43126 mark as,follows
R10801 T43126 T43112 advcl follows,designed
R10802 T43127 T43112 punct .,designed
R10803 T43129 T43130 punct (,1
R10804 T43130 T43131 meta 1,placed
R10805 T43132 T43130 punct ),1
R10806 T43133 T43134 amod Inclined,screen
R10807 T43134 T43135 nmod screen,tests
R10808 T43135 T43131 dep tests,placed
R10809 T43136 T43134 cc and,screen
R10810 T43137 T43138 amod inverted,screen
R10811 T43138 T43134 conj screen,screen
R10812 T43139 T43131 punct : ,placed
R10813 T43140 T43131 prep For,placed
R10814 T43141 T43142 det the,tests
R10815 T43142 T43140 pobj tests,For
R10816 T43143 T43142 amod inclined,tests
R10817 T43144 T43142 compound screen,tests
R10818 T43145 T43131 punct ", ",placed
R10819 T43146 T43147 det each,mouse
R10820 T43147 T43131 nsubjpass mouse,placed
R10821 T43148 T43131 auxpass was,placed
R10822 T43149 T43131 prep on,placed
R10823 T43150 T43149 pobj top,on
R10824 T43151 T43150 prep of,top
R10825 T43152 T43153 det an,grid
R10826 T43153 T43151 pobj grid,of
R10827 T43154 T43153 amod elevated,grid
R10828 T43155 T43156 punct (,above
R10829 T43156 T43154 parataxis above,elevated
R10830 T43157 T43158 nummod 47,cm
R10831 T43158 T43156 npadvmod cm,above
R10832 T43159 T43160 det the,floor
R10833 T43160 T43156 pobj floor,above
R10834 T43161 T43156 punct ),above
R10835 T43162 T43163 compound wire,mesh
R10836 T43163 T43153 compound mesh,grid
R10837 T43164 T43165 punct (,squares
R10838 T43165 T43153 parataxis squares,grid
R10839 T43166 T43165 nummod 16,squares
R10840 T43167 T43165 prep per,squares
R10841 T43168 T43169 nummod 10,cm
R10842 T43169 T43167 pobj cm,per
R10843 T43170 T43165 punct ),squares
R10844 T43171 T43172 dep that,inclined
R10845 T43172 T43153 relcl inclined,grid
R10846 T43173 T43172 auxpass was,inclined
R10847 T43174 T43172 prep to,inclined
R10848 T43175 T43176 nummod 60,°
R10849 T43176 T43174 pobj °,to
R10850 T43177 T43176 cc or,°
R10851 T43178 T43179 nummod 90,°
R10852 T43179 T43176 conj °,°
R10853 T43180 T43131 punct .,placed
R10854 T43182 T43183 det Each,animal
R10855 T43183 T43184 nsubjpass animal,placed
R10856 T43185 T43184 auxpass was,placed
R10857 T43186 T43184 prep in,placed
R10858 T43187 T43188 det the,middle
R10859 T43188 T43186 pobj middle,in
R10860 T43189 T43188 prep of,middle
R10861 T43190 T43191 det the,screen
R10862 T43191 T43189 pobj screen,of
R10863 T43192 T43193 mark with,oriented
R10864 T43193 T43184 advcl oriented,placed
R10865 T43194 T43195 poss its,head
R10866 T43195 T43193 nsubj head,oriented
R10867 T43196 T43193 advmod down,oriented
R10868 T43197 T43184 cc and,placed
R10869 T43198 T43199 auxpass was,timed
R10870 T43199 T43184 conj timed,placed
R10871 T43200 T43199 prep for,timed
R10872 T43201 T43202 advmod how,long
R10873 T43202 T43203 advmod long,remained
R10874 T43203 T43200 pobj remained,for
R10875 T43204 T43203 nsubj it,remained
R10876 T43205 T43203 prep on,remained
R10877 T43206 T43207 det the,screen
R10878 T43207 T43205 pobj screen,on
R10879 T43208 T43203 cc and,remained
R10880 T43209 T43210 advmod how,long
R10881 T43210 T43211 advmod long,took
R10882 T43211 T43203 conj took,remained
R10883 T43212 T43211 nsubj it,took
R10884 T43213 T43214 aux to,climb
R10885 T43214 T43211 advcl climb,took
R10886 T43215 T43214 prep to,climb
R10887 T43216 T43217 det the,top
R10888 T43217 T43215 pobj top,to
R10889 T43218 T43217 prep of,top
R10890 T43219 T43220 det the,screen
R10891 T43220 T43218 pobj screen,of
R10892 T43221 T43184 punct .,placed
R10893 T43223 T43224 prep For,placed
R10894 T43225 T43226 det the,test
R10895 T43226 T43223 pobj test,For
R10896 T43227 T43226 amod inverted,test
R10897 T43228 T43226 compound screen,test
R10898 T43229 T43224 punct ", ",placed
R10899 T43230 T43224 nsubjpass mice,placed
R10900 T43231 T43224 auxpass were,placed
R10901 T43232 T43224 prep as,placed
R10902 T43233 T43232 pcomp above,as
R10903 T43234 T43224 cc and,placed
R10904 T43235 T43236 advmod then,inverted
R10905 T43236 T43224 conj inverted,placed
R10906 T43237 T43238 det the,screen
R10907 T43238 T43236 nsubjpass screen,inverted
R10908 T43239 T43236 auxpass was,inverted
R10909 T43240 T43236 prep to,inverted
R10910 T43241 T43242 nummod 180,°
R10911 T43242 T43240 pobj °,to
R10912 T43243 T43236 punct .,inverted
R10913 T43245 T43246 det A,score
R10914 T43246 T43248 nsubjpass score,given
R10915 T43247 T43246 amod maximum,score
R10916 T43249 T43246 prep of,score
R10917 T43250 T43251 nummod 60,s
R10918 T43251 T43249 pobj s,of
R10919 T43252 T43248 auxpass was,given
R10920 T43253 T43254 mark if,fall
R10921 T43254 T43248 advcl fall,given
R10922 T43255 T43256 det an,animal
R10923 T43256 T43254 nsubj animal,fall
R10924 T43257 T43254 aux did,fall
R10925 T43258 T43254 neg not,fall
R10926 T43259 T43248 punct ;,given
R10927 T43261 T43262 punct (,2
R10928 T43262 T43263 meta 2,timed
R10929 T43264 T43262 punct ),2
R10930 T43265 T43266 compound Platform,test
R10931 T43266 T43263 dep test,timed
R10932 T43267 T43263 punct : ,timed
R10933 T43268 T43269 det Each,mouse
R10934 T43269 T43263 nsubjpass mouse,timed
R10935 T43270 T43263 auxpass was,timed
R10936 T43271 T43263 prep for,timed
R10937 T43272 T43273 advmod how,long
R10938 T43273 T43274 advmod long,remained
R10939 T43274 T43271 pcomp remained,for
R10940 T43275 T43274 nsubj it,remained
R10941 T43276 T43274 prep on,remained
R10942 T43277 T43278 det an,platform
R10943 T43278 T43276 pobj platform,on
R10944 T43279 T43278 amod elevated,platform
R10945 T43280 T43281 punct (,above
R10946 T43281 T43279 parataxis above,elevated
R10947 T43282 T43283 nummod 47,cm
R10948 T43283 T43281 npadvmod cm,above
R10949 T43284 T43285 det the,floor
R10950 T43285 T43281 pobj floor,above
R10951 T43286 T43281 punct ),above
R10952 T43287 T43278 amod circular,platform
R10953 T43288 T43289 punct (,thick
R10954 T43289 T43278 parataxis thick,platform
R10955 T43290 T43291 nummod 1.0,cm
R10956 T43291 T43289 npadvmod cm,thick
R10957 T43292 T43289 cc and,thick
R10958 T43293 T43294 nummod 3.0,cm
R10959 T43294 T43289 conj cm,thick
R10960 T43295 T43294 prep in,cm
R10961 T43296 T43295 pobj diameter,in
R10962 T43297 T43289 punct ),thick
R10963 T43298 T43263 punct .,timed
R10964 T43300 T43301 det A,score
R10965 T43301 T43303 nsubjpass score,assigned
R10966 T43302 T43301 amod maximum,score
R10967 T43304 T43301 prep of,score
R10968 T43305 T43306 nummod 60,s
R10969 T43306 T43304 pobj s,of
R10970 T43307 T43303 auxpass was,assigned
R10971 T43308 T43309 mark if,remained
R10972 T43309 T43303 advcl remained,assigned
R10973 T43310 T43311 det the,mouse
R10974 T43311 T43309 nsubj mouse,remained
R10975 T43312 T43309 prep on,remained
R10976 T43313 T43314 det the,platform
R10977 T43314 T43312 pobj platform,on
R10978 T43315 T43309 prep for,remained
R10979 T43316 T43317 det the,amount
R10980 T43317 T43315 pobj amount,for
R10981 T43318 T43317 compound maximum,amount
R10982 T43319 T43317 prep of,amount
R10983 T43320 T43319 pobj time,of
R10984 T43321 T43309 cc or,remained
R10985 T43322 T43323 mark if,climb
R10986 T43323 T43309 conj climb,remained
R10987 T43324 T43323 nsubj it,climb
R10988 T43325 T43323 aux could,climb
R10989 T43326 T43323 advmod down,climb
R10990 T43327 T43323 prep on,climb
R10991 T43328 T43329 det a,pole
R10992 T43329 T43327 pobj pole,on
R10993 T43330 T43331 advmod very,thin
R10994 T43331 T43329 amod thin,pole
R10995 T43332 T43333 dep that,supported
R10996 T43333 T43329 relcl supported,pole
R10997 T43334 T43335 det the,platform
R10998 T43335 T43333 dobj platform,supported
R10999 T43336 T43323 punct ", ",climb
R11000 T43337 T43323 prep without,climb
R11001 T43338 T43337 pcomp falling,without
R11002 T43339 T43303 punct ;,assigned
R11003 T43341 T43342 punct (,3
R11004 T43342 T43343 meta 3,timed
R11005 T43344 T43342 punct ),3
R11006 T43345 T43346 compound Ledge,test
R11007 T43346 T43343 dep test,timed
R11008 T43347 T43343 punct : ,timed
R11009 T43348 T43349 det Each,mouse
R11010 T43349 T43343 nsubjpass mouse,timed
R11011 T43350 T43343 auxpass was,timed
R11012 T43351 T43343 prep for,timed
R11013 T43352 T43353 advmod how,long
R11014 T43353 T43354 advmod long,maintain
R11015 T43354 T43351 pcomp maintain,for
R11016 T43355 T43354 nsubj it,maintain
R11017 T43356 T43354 aux could,maintain
R11018 T43357 T43358 poss its,balance
R11019 T43358 T43354 dobj balance,maintain
R11020 T43359 T43354 prep on,maintain
R11021 T43360 T43361 det a,ledge
R11022 T43361 T43359 pobj ledge,on
R11023 T43362 T43363 nummod 0.75,cm
R11024 T43363 T43365 npadvmod cm,wide
R11025 T43364 T43363 punct -,cm
R11026 T43365 T43361 amod wide,ledge
R11027 T43366 T43361 compound Plexiglas,ledge
R11028 T43367 T43354 prep without,maintain
R11029 T43368 T43367 pcomp falling,without
R11030 T43369 T43370 punct (,maximum
R11031 T43370 T43354 parataxis maximum,maintain
R11032 T43371 T43372 nummod 60,s
R11033 T43372 T43370 compound s,maximum
R11034 T43373 T43370 punct ),maximum
R11035 T43374 T43343 punct .,timed
R11036 T43376 T43377 det A,score
R11037 T43377 T43378 nsubjpass score,assigned
R11038 T43379 T43377 prep of,score
R11039 T43380 T43381 nummod 60,s
R11040 T43381 T43379 pobj s,of
R11041 T43382 T43378 auxpass was,assigned
R11042 T43383 T43378 advmod also,assigned
R11043 T43384 T43385 mark if,traversed
R11044 T43385 T43378 advcl traversed,assigned
R11045 T43386 T43387 det the,mouse
R11046 T43387 T43385 nsubj mouse,traversed
R11047 T43388 T43389 det the,length
R11048 T43389 T43385 dobj length,traversed
R11049 T43390 T43389 amod entire,length
R11050 T43391 T43389 punct (,length
R11051 T43392 T43393 nummod 51,cm
R11052 T43393 T43389 appos cm,length
R11053 T43394 T43389 punct ),length
R11054 T43395 T43389 prep of,length
R11055 T43396 T43397 det the,ledge
R11056 T43397 T43395 pobj ledge,of
R11057 T43398 T43397 compound Plexiglas,ledge
R11058 T43399 T43385 cc and,traversed
R11059 T43400 T43385 conj returned,traversed
R11060 T43401 T43400 prep to,returned
R11061 T43402 T43403 det the,place
R11062 T43403 T43401 pobj place,to
R11063 T43404 T43403 compound starting,place
R11064 T43405 T43400 prep in,returned
R11065 T43406 T43407 amod less,60
R11066 T43407 T43409 nummod 60,s
R11067 T43408 T43407 quantmod than,60
R11068 T43409 T43405 pobj s,in
R11069 T43410 T43400 prep without,returned
R11070 T43411 T43410 pcomp falling,without
R11071 T43412 T43378 punct ;,assigned
R11072 T43414 T43415 punct (,4
R11073 T43415 T43416 meta 4,test
R11074 T43417 T43415 punct ),4
R11075 T43418 T43416 compound Walking,test
R11076 T43419 T43416 compound initiation,test
R11077 T43420 T43416 punct : ,test
R11078 T43421 T43422 det Each,mouse
R11079 T43422 T43423 nsubjpass mouse,placed
R11080 T43423 T43416 conj placed,test
R11081 T43424 T43423 auxpass was,placed
R11082 T43425 T43423 prep in,placed
R11083 T43426 T43427 det the,middle
R11084 T43427 T43425 pobj middle,in
R11085 T43428 T43427 prep of,middle
R11086 T43429 T43430 det a,square
R11087 T43430 T43428 pobj square,of
R11088 T43431 T43430 acl outlined,square
R11089 T43432 T43431 agent by,outlined
R11090 T43433 T43434 amod white,tape
R11091 T43434 T43432 pobj tape,by
R11092 T43435 T43434 compound cloth,tape
R11093 T43436 T43437 punct (,cm
R11094 T43437 T43434 parataxis cm,tape
R11095 T43438 T43437 nummod 21,cm
R11096 T43439 T43440 punct ×,cm
R11097 T43440 T43437 prep cm,cm
R11098 T43441 T43440 nummod 21,cm
R11099 T43442 T43437 punct ),cm
R11100 T43443 T43423 prep on,placed
R11101 T43444 T43445 det a,surface
R11102 T43445 T43443 pobj surface,on
R11103 T43446 T43445 amod smooth,surface
R11104 T43447 T43445 amod black,surface
R11105 T43448 T43445 prep of,surface
R11106 T43449 T43450 det a,tabletop
R11107 T43450 T43448 pobj tabletop,of
R11108 T43451 T43450 amod large,tabletop
R11109 T43452 T43423 cc .,placed
R11110 T43454 T43455 det The,time
R11111 T43455 T43456 nsubjpass time,recorded
R11112 T43457 T43458 nsubj it,took
R11113 T43458 T43455 advcl took,time
R11114 T43459 T43460 det each,mouse
R11115 T43460 T43461 nsubj mouse,leave
R11116 T43461 T43458 ccomp leave,took
R11117 T43462 T43461 aux to,leave
R11118 T43463 T43464 det the,square
R11119 T43464 T43461 dobj square,leave
R11120 T43465 T43461 punct (,leave
R11121 T43466 T43461 dep place,leave
R11122 T43467 T43468 det all,paws
R11123 T43468 T43466 dobj paws,place
R11124 T43469 T43468 nummod four,paws
R11125 T43470 T43466 advmod outside,place
R11126 T43471 T43470 prep of,outside
R11127 T43472 T43473 det the,tape
R11128 T43473 T43471 pobj tape,of
R11129 T43474 T43456 punct ),recorded
R11130 T43475 T43456 auxpass was,recorded
R11131 T43476 T43456 punct .,recorded
R11132 T43478 T43479 det The,time
R11133 T43479 T43481 nsubj time,was
R11134 T43480 T43479 amod maximum,time
R11135 T43482 T43479 acl allowed,time
R11136 T43483 T43484 nummod 60,s
R11137 T43484 T43481 attr s,was
R11138 T43485 T43481 punct .,was
R11139 T43487 T43488 quantmod 6,8
R11140 T43488 T43490 nummod 8,mo
R11141 T43489 T43488 punct –,8
R11142 T43490 T43492 npadvmod mo,old
R11143 T43491 T43490 punct -,mo
R11144 T43492 T43494 amod old,mice
R11145 T43493 T43492 punct -,old
R11146 T43494 T43518 nsubjpass mice,run
R11147 T43495 T43496 nmod PGC,1α
R11148 T43496 T43494 nmod 1α,mice
R11149 T43497 T43496 punct -,1α
R11150 T43498 T43496 punct +,1α
R11151 T43499 T43496 punct /,1α
R11152 T43500 T43496 punct +,1α
R11153 T43501 T43502 punct (,4
R11154 T43502 T43496 parataxis 4,1α
R11155 T43503 T43502 nsubj n,4
R11156 T43504 T43502 punct =,4
R11157 T43505 T43502 punct ),4
R11158 T43506 T43496 cc and,1α
R11159 T43507 T43508 compound PGC,1α
R11160 T43508 T43496 conj 1α,1α
R11161 T43509 T43508 punct -,1α
R11162 T43510 T43508 punct −,1α
R11163 T43511 T43508 punct /,1α
R11164 T43512 T43508 punct −,1α
R11165 T43513 T43514 punct (,8
R11166 T43514 T43508 parataxis 8,1α
R11167 T43515 T43514 nsubj n,8
R11168 T43516 T43514 punct =,8
R11169 T43517 T43514 punct ),8
R11170 T43519 T43518 auxpass were,run
R11171 T43520 T43518 prep to,run
R11172 T43521 T43520 pobj exhaustion,to
R11173 T43522 T43518 advcl employing,run
R11174 T43523 T43524 det a,system
R11175 T43524 T43522 dobj system,employing
R11176 T43525 T43524 amod motorized,system
R11177 T43526 T43524 punct ", ",system
R11178 T43527 T43528 npadvmod speed,controlled
R11179 T43528 T43524 amod controlled,system
R11180 T43529 T43524 punct ", ",system
R11181 T43530 T43524 amod modular,system
R11182 T43531 T43524 compound treadmill,system
R11183 T43532 T43533 punct (,Instruments
R11184 T43533 T43524 parataxis Instruments,system
R11185 T43534 T43533 compound Columbus,Instruments
R11186 T43535 T43533 punct ),Instruments
R11187 T43536 T43518 punct .,run
R11188 T43538 T43539 det The,treadmill
R11189 T43539 T43540 nsubjpass treadmill,equipped
R11190 T43541 T43540 auxpass was,equipped
R11191 T43542 T43540 prep with,equipped
R11192 T43543 T43544 det an,stimulus
R11193 T43544 T43542 pobj stimulus,with
R11194 T43545 T43546 amod electric,shock
R11195 T43546 T43544 compound shock,stimulus
R11196 T43547 T43544 cc and,stimulus
R11197 T43548 T43549 det an,angle
R11198 T43549 T43544 conj angle,stimulus
R11199 T43550 T43549 amod adjustable,angle
R11200 T43551 T43549 compound inclination,angle
R11201 T43552 T43540 punct .,equipped
R11202 T43554 T43555 compound Running,velocity
R11203 T43555 T43556 nsubjpass velocity,set
R11204 T43557 T43556 auxpass was,set
R11205 T43558 T43556 prep at,set
R11206 T43559 T43560 nummod 35,m
R11207 T43560 T43558 pobj m,at
R11208 T43561 T43562 punct /,min
R11209 T43562 T43560 prep min,m
R11210 T43563 T43556 punct ", ",set
R11211 T43564 T43556 prep with,set
R11212 T43565 T43566 det a,angle
R11213 T43566 T43564 pobj angle,with
R11214 T43567 T43566 compound level,angle
R11215 T43568 T43566 compound inclination,angle
R11216 T43569 T43556 punct .,set
R11217 T44022 T44023 compound VO2max,studies
R11218 T44025 T44026 nsubjpass VO2max,determined
R11219 T44027 T44026 auxpass was,determined
R11220 T44028 T44029 mark while,running
R11221 T44029 T44026 advcl running,determined
R11222 T44030 T44031 det the,mice
R11223 T44031 T44029 nsubj mice,running
R11224 T44032 T44029 aux were,running
R11225 T44033 T44029 prep on,running
R11226 T44034 T44035 det a,treadmill
R11227 T44035 T44033 pobj treadmill,on
R11228 T44036 T44026 advcl using,determined
R11229 T44037 T44038 det an,system
R11230 T44038 T44036 dobj system,using
R11231 T44039 T44040 amod open,flow
R11232 T44040 T44038 compound flow,system
R11233 T44041 T44042 punct (,System
R11234 T44042 T44038 parataxis System,system
R11235 T44043 T44042 compound Columbus,System
R11236 T44044 T44042 compound Instruments,System
R11237 T44045 T44042 compound Oxymax,System
R11238 T44046 T44042 punct ),System
R11239 T44047 T44026 punct .,determined
R11240 T44049 T44050 det All,measurements
R11241 T44050 T44051 nsubj measurements,took
R11242 T44052 T44050 prep of,measurements
R11243 T44053 T44054 compound oxygen,consumption
R11244 T44054 T44052 pobj consumption,of
R11245 T44055 T44051 dobj place,took
R11246 T44056 T44051 prep at,took
R11247 T44057 T44058 det an,elevation
R11248 T44058 T44056 pobj elevation,at
R11249 T44059 T44058 prep of,elevation
R11250 T44060 T44061 nummod 150,m
R11251 T44061 T44059 pobj m,of
R11252 T44062 T44063 punct (,torr
R11253 T44063 T44051 parataxis torr,took
R11254 T44064 T44065 compound ambient,PBAR
R11255 T44065 T44063 nsubj PBAR,torr
R11256 T44066 T44063 punct =,torr
R11257 T44067 T44063 nummod 745,torr
R11258 T44068 T44063 punct ),torr
R11259 T44069 T44051 punct .,took
R11260 T44071 T44072 nsubjpass Animals,placed
R11261 T44073 T44072 auxpass were,placed
R11262 T44074 T44072 prep into,placed
R11263 T44075 T44076 det the,chamber
R11264 T44076 T44074 pobj chamber,into
R11265 T44077 T44076 amod metabolic,chamber
R11266 T44078 T44072 prep for,placed
R11267 T44079 T44080 quantmod 3,5
R11268 T44080 T44082 nummod 5,min
R11269 T44081 T44080 punct –,5
R11270 T44082 T44078 pobj min,for
R11271 T44083 T44084 aux to,allow
R11272 T44084 T44072 advcl allow,placed
R11273 T44085 T44086 det the,system
R11274 T44086 T44084 dobj system,allow
R11275 T44087 T44088 aux to,equilibrate
R11276 T44088 T44084 xcomp equilibrate,allow
R11277 T44089 T44072 punct .,placed
R11278 T44091 T44092 nsubjpass Mice,induced
R11279 T44093 T44092 auxpass were,induced
R11280 T44094 T44092 advmod then,induced
R11281 T44095 T44096 aux to,run
R11282 T44096 T44092 xcomp run,induced
R11283 T44097 T44096 prep up,run
R11284 T44098 T44099 det an,incline
R11285 T44099 T44097 pobj incline,up
R11286 T44100 T44101 nummod 18,°
R11287 T44101 T44099 compound °,incline
R11288 T44102 T44096 prep at,run
R11289 T44103 T44104 det a,speed
R11290 T44104 T44102 pobj speed,at
R11291 T44105 T44104 prep of,speed
R11292 T44106 T44107 nummod 40,m
R11293 T44107 T44105 pobj m,of
R11294 T44108 T44109 punct /,min
R11295 T44109 T44107 prep min,m
R11296 T44110 T44096 advcl using,run
R11297 T44111 T44112 det a,grid
R11298 T44112 T44110 dobj grid,using
R11299 T44113 T44112 compound shock,grid
R11300 T44114 T44112 prep in,grid
R11301 T44115 T44116 det the,rear
R11302 T44116 T44114 pobj rear,in
R11303 T44117 T44116 prep of,rear
R11304 T44118 T44119 det the,chamber
R11305 T44119 T44117 pobj chamber,of
R11306 T44120 T44092 punct .,induced
R11307 T44122 T44123 det The,speed
R11308 T44123 T44124 nsubjpass speed,increased
R11309 T44125 T44124 auxpass was,increased
R11310 T44126 T44124 prep by,increased
R11311 T44127 T44128 nummod 5,m
R11312 T44128 T44126 pobj m,by
R11313 T44129 T44130 punct /,min
R11314 T44130 T44128 prep min,m
R11315 T44131 T44132 det every,min
R11316 T44132 T44124 npadvmod min,increased
R11317 T44133 T44132 nummod 2,min
R11318 T44134 T44135 mark until,were
R11319 T44135 T44124 advcl were,increased
R11320 T44136 T44137 det the,animals
R11321 T44137 T44135 nsubj animals,were
R11322 T44138 T44135 acomp unable,were
R11323 T44139 T44140 aux to,continue
R11324 T44140 T44138 xcomp continue,unable
R11325 T44141 T44124 punct .,increased
R11326 T44143 T44144 amod Maximal,effort
R11327 T44144 T44145 nsubjpass effort,determined
R11328 T44146 T44145 auxpass was,determined
R11329 T44147 T44148 advmod when,increase
R11330 T44148 T44145 advcl increase,determined
R11331 T44149 T44150 compound oxygen,uptake
R11332 T44150 T44148 nsubj uptake,increase
R11333 T44151 T44148 aux did,increase
R11334 T44152 T44148 neg not,increase
R11335 T44153 T44148 prep with,increase
R11336 T44154 T44155 compound power,output
R11337 T44155 T44153 pobj output,with
R11338 T44156 T44148 cc and,increase
R11339 T44157 T44158 advmod subsequently,failed
R11340 T44158 T44148 conj failed,increase
R11341 T44159 T44160 det the,mouse
R11342 T44160 T44158 nsubj mouse,failed
R11343 T44161 T44162 aux to,maintain
R11344 T44162 T44158 xcomp maintain,failed
R11345 T44163 T44162 dobj effort,maintain
R11346 T44164 T44145 punct .,determined
R11347 T44166 T44167 nsubjpass VO2max,calculated
R11348 T44168 T44167 auxpass was,calculated
R11349 T44169 T44167 advcl using,calculated
R11350 T44170 T44171 det the,values
R11351 T44171 T44169 dobj values,using
R11352 T44172 T44171 amod averaged,values
R11353 T44173 T44169 prep over,using
R11354 T44174 T44175 nummod 1,min
R11355 T44175 T44173 pobj min,over
R11356 T44176 T44177 prep during,reached
R11357 T44177 T44175 relcl reached,min
R11358 T44178 T44176 pobj which,during
R11359 T44179 T44180 det the,animal
R11360 T44180 T44181 poss animal,consumption
R11361 T44181 T44177 nsubj consumption,reached
R11362 T44182 T44180 case 's,animal
R11363 T44183 T44181 compound O2,consumption
R11364 T44184 T44185 det a,plateau
R11365 T44185 T44177 dobj plateau,reached
R11366 T44186 T44167 punct .,calculated
R11367 T44806 T44807 amod Isolated,studies
R11368 T44808 T44807 compound muscle,studies
R11369 T44809 T44807 compound stimulation,studies
R11370 T44811 T44812 nsubjpass Animals,anesthetized
R11371 T44813 T44812 auxpass were,anesthetized
R11372 T44814 T44812 prep with,anesthetized
R11373 T44815 T44816 compound ketamine,xylazine
R11374 T44816 T44814 pobj xylazine,with
R11375 T44817 T44816 punct /,xylazine
R11376 T44818 T44812 cc and,anesthetized
R11377 T44819 T44820 det the,muscle
R11378 T44820 T44822 nsubjpass muscle,removed
R11379 T44821 T44820 compound soleus,muscle
R11380 T44822 T44812 conj removed,anesthetized
R11381 T44823 T44822 auxpass was,removed
R11382 T44824 T44822 prep from,removed
R11383 T44825 T44826 nummod one,leg
R11384 T44826 T44824 pobj leg,from
R11385 T44827 T44822 punct .,removed
R11386 T44829 T44830 prep Upon,suspended
R11387 T44831 T44829 pobj removal,Upon
R11388 T44832 T44830 punct ", ",suspended
R11389 T44833 T44834 det the,muscle
R11390 T44834 T44830 nsubjpass muscle,suspended
R11391 T44835 T44830 auxpass was,suspended
R11392 T44836 T44830 prep in,suspended
R11393 T44837 T44838 det a,solution
R11394 T44838 T44836 pobj solution,in
R11395 T44839 T44838 compound Krebs,solution
R11396 T44840 T44838 acl aerated,solution
R11397 T44841 T44840 prep with,aerated
R11398 T44842 T44843 nummod 95,%
R11399 T44843 T44844 compound %,O2
R11400 T44844 T44841 pobj O2,with
R11401 T44845 T44844 cc and,O2
R11402 T44846 T44847 nummod 5,%
R11403 T44847 T44848 compound %,CO2
R11404 T44848 T44844 conj CO2,O2
R11405 T44849 T44830 punct .,suspended
R11406 T44851 T44852 det The,muscle
R11407 T44852 T44853 nsubjpass muscle,suspended
R11408 T44854 T44852 cc and,muscle
R11409 T44855 T44856 compound Krebs,solution
R11410 T44856 T44852 conj solution,muscle
R11411 T44857 T44853 auxpass were,suspended
R11412 T44858 T44853 prep within,suspended
R11413 T44859 T44860 det a,bath
R11414 T44860 T44858 pobj bath,within
R11415 T44861 T44860 compound water,bath
R11416 T44862 T44860 acl maintained,bath
R11417 T44863 T44862 prep at,maintained
R11418 T44864 T44865 nummod 37,°C
R11419 T44865 T44863 pobj °C,at
R11420 T44866 T44853 punct ", ",suspended
R11421 T44867 T44853 cc and,suspended
R11422 T44868 T44869 det the,muscle
R11423 T44869 T44870 nsubjpass muscle,anchored
R11424 T44870 T44853 conj anchored,suspended
R11425 T44871 T44870 auxpass was,anchored
R11426 T44872 T44870 prep to,anchored
R11427 T44873 T44874 det a,transducer
R11428 T44874 T44872 pobj transducer,to
R11429 T44875 T44874 nmod Grass,transducer
R11430 T44876 T44877 punct (,Warwick
R11431 T44877 T44875 parataxis Warwick,Grass
R11432 T44878 T44877 compound West,Warwick
R11433 T44879 T44877 punct ", ",Warwick
R11434 T44880 T44881 compound Rhode,Island
R11435 T44881 T44877 npadvmod Island,Warwick
R11436 T44882 T44877 punct ", ",Warwick
R11437 T44883 T44884 compound United,States
R11438 T44884 T44877 npadvmod States,Warwick
R11439 T44885 T44877 punct ),Warwick
R11440 T44886 T44874 amod isometric,transducer
R11441 T44887 T44874 compound force,transducer
R11442 T44888 T44889 punct (,FTO3C
R11443 T44889 T44874 parataxis FTO3C,transducer
R11444 T44890 T44889 compound model,FTO3C
R11445 T44891 T44889 punct ),FTO3C
R11446 T44892 T44870 punct .,anchored
R11447 T44894 T44895 nsubjpass Muscles,stimulated
R11448 T44896 T44895 auxpass were,stimulated
R11449 T44897 T44898 aux to,contract
R11450 T44898 T44895 advcl contract,stimulated
R11451 T44899 T44895 prep with,stimulated
R11452 T44900 T44901 det a,stimulator
R11453 T44901 T44899 pobj stimulator,with
R11454 T44902 T44901 compound Grass,stimulator
R11455 T44903 T44904 punct (,S88
R11456 T44904 T44901 parataxis S88,stimulator
R11457 T44905 T44904 compound model,S88
R11458 T44906 T44904 punct ),S88
R11459 T44907 T44901 acl generating,stimulator
R11460 T44908 T44909 det a,stimulus
R11461 T44909 T44907 dobj stimulus,generating
R11462 T44910 T44909 compound field,stimulus
R11463 T44911 T44907 prep through,generating
R11464 T44912 T44911 pobj electrodes,through
R11465 T44913 T44912 acl located,electrodes
R11466 T44914 T44913 prep at,located
R11467 T44915 T44916 det both,ends
R11468 T44916 T44914 pobj ends,at
R11469 T44917 T44916 prep of,ends
R11470 T44918 T44919 det the,muscle
R11471 T44919 T44917 pobj muscle,of
R11472 T44920 T44895 punct .,stimulated
R11473 T44922 T44923 nmod Force,voltage
R11474 T44923 T44925 nmod voltage,relationships
R11475 T44924 T44923 punct -,voltage
R11476 T44925 T44938 nsubjpass relationships,determined
R11477 T44926 T44923 punct (,voltage
R11478 T44927 T44928 amod maximal,force
R11479 T44928 T44923 appos force,voltage
R11480 T44929 T44928 prep at,force
R11481 T44930 T44931 amod about,100
R11482 T44931 T44932 nummod 100,V
R11483 T44932 T44929 pobj V,at
R11484 T44933 T44923 punct ),voltage
R11485 T44934 T44923 cc and,voltage
R11486 T44935 T44936 compound length,tension
R11487 T44936 T44923 conj tension,voltage
R11488 T44937 T44936 punct -,tension
R11489 T44939 T44938 auxpass were,determined
R11490 T44940 T44938 advcl using,determined
R11491 T44941 T44942 amod single,twitch
R11492 T44942 T44943 compound twitch,stimuli
R11493 T44943 T44940 dobj stimuli,using
R11494 T44944 T44938 punct .,determined
R11495 T44946 T44947 det The,stimulator
R11496 T44947 T44948 nsubj stimulator,delivered
R11497 T44949 T44948 advmod then,delivered
R11498 T44950 T44951 amod repeating,trains
R11499 T44951 T44948 dobj trains,delivered
R11500 T44952 T44951 prep of,trains
R11501 T44953 T44952 pobj stimuli,of
R11502 T44954 T44948 prep at,delivered
R11503 T44955 T44954 pobj one,at
R11504 T44956 T44955 prep per,one
R11505 T44957 T44956 pobj second,per
R11506 T44958 T44948 prep at,delivered
R11507 T44959 T44960 nummod 40,Hz
R11508 T44960 T44958 pobj Hz,at
R11509 T44961 T44948 prep for,delivered
R11510 T44962 T44963 nummod 2,min
R11511 T44963 T44961 pobj min,for
R11512 T44964 T44948 punct .,delivered
R11513 T44966 T44967 det Each,train
R11514 T44967 T44968 nsubj train,lasted
R11515 T44969 T44970 nummod 330,ms
R11516 T44970 T44968 npadvmod ms,lasted
R11517 T44971 T44968 punct ", ",lasted
R11518 T44972 T44968 cc and,lasted
R11519 T44973 T44974 auxpass were,recorded
R11520 T44974 T44968 conj recorded,lasted
R11521 T44975 T44974 advmod digitally,recorded
R11522 T44976 T44974 advcl using,recorded
R11523 T44977 T44976 dobj MacLab,using
R11524 T44978 T44979 punct (,Instruments
R11525 T44979 T44977 parataxis Instruments,MacLab
R11526 T44980 T44979 compound AD,Instruments
R11527 T44981 T44979 punct ", ",Instruments
R11528 T44982 T44983 compound Colorado,Springs
R11529 T44983 T44979 npadvmod Springs,Instruments
R11530 T44984 T44979 punct ", ",Instruments
R11531 T44985 T44979 npadvmod Colorado,Instruments
R11532 T44986 T44979 punct ", ",Instruments
R11533 T44987 T44988 compound United,States
R11534 T44988 T44979 npadvmod States,Instruments
R11535 T44989 T44979 punct ),Instruments
R11536 T44990 T44968 punct .,lasted
R11537 T44992 T44993 compound Fatigue,resistance
R11538 T44993 T44994 nsubjpass resistance,calculated
R11539 T44995 T44994 auxpass was,calculated
R11540 T44996 T44994 prep as,calculated
R11541 T44997 T44998 det the,ratio
R11542 T44998 T44996 pobj ratio,as
R11543 T44999 T44998 prep of,ratio
R11544 T45000 T45001 det the,force
R11545 T45001 T44999 pobj force,of
R11546 T45002 T45001 acl generated,force
R11547 T45003 T45002 agent by,generated
R11548 T45004 T45005 det the,tetanus
R11549 T45005 T45003 pobj tetanus,by
R11550 T45006 T45005 amod last,tetanus
R11551 T45007 T45001 acl divided,force
R11552 T45008 T45007 prep by,divided
R11553 T45009 T45010 det the,force
R11554 T45010 T45008 pobj force,by
R11555 T45011 T45010 amod highest,force
R11556 T45012 T45010 acl generated,force
R11557 T45013 T45001 acl multiplied,force
R11558 T45014 T45013 prep by,multiplied
R11559 T45015 T45014 pobj 100,by
R11560 T45016 T45017 aux to,give
R11561 T45017 T44994 advcl give,calculated
R11562 T45018 T45019 det the,percent
R11563 T45019 T45017 dobj percent,give
R11564 T45020 T45019 prep of,percent
R11565 T45021 T45022 compound force,generation
R11566 T45022 T45020 pobj generation,of
R11567 T45023 T45024 dep that,remained
R11568 T45024 T45019 relcl remained,percent
R11569 T45025 T45024 prep after,remained
R11570 T45026 T45027 det the,protocol
R11571 T45027 T45025 pobj protocol,after
R11572 T45028 T45027 compound fatigue,protocol
R11573 T45029 T44994 punct .,calculated
R11574 T45215 T45216 compound Exercise,echocardiography
R11575 T45218 T45219 amod Adult,mice
R11576 T45219 T45221 nsubjpass mice,exercised
R11577 T45220 T45219 amod female,mice
R11578 T45222 T45219 punct (,mice
R11579 T45223 T45224 quantmod 6,8
R11580 T45224 T45226 nummod 8,mo
R11581 T45225 T45224 punct –,8
R11582 T45226 T45227 npadvmod mo,old
R11583 T45227 T45219 amod old,mice
R11584 T45228 T45221 punct ),exercised
R11585 T45229 T45221 auxpass were,exercised
R11586 T45230 T45221 prep on,exercised
R11587 T45231 T45232 det the,treadmill
R11588 T45232 T45230 pobj treadmill,on
R11589 T45233 T45232 amod motorized,treadmill
R11590 T45234 T45221 advcl using,exercised
R11591 T45235 T45236 det the,settings
R11592 T45236 T45234 dobj settings,using
R11593 T45237 T45236 nmod run,settings
R11594 T45238 T45237 punct -,run
R11595 T45239 T45237 prep to,run
R11596 T45240 T45239 punct -,to
R11597 T45241 T45239 pobj exhaustion,to
R11598 T45242 T45236 acl described,settings
R11599 T45243 T45242 advmod above,described
R11600 T45244 T45234 prep for,using
R11601 T45245 T45246 nummod 60,s
R11602 T45246 T45244 pobj s,for
R11603 T45247 T45244 cc or,for
R11604 T45248 T45244 conj until,for
R11605 T45249 T45248 pobj exhaustion,until
R11606 T45250 T45251 punct ", ",came
R11607 T45251 T45248 parataxis came,until
R11608 T45252 T45251 dep whichever,came
R11609 T45253 T45251 advmod first,came
R11610 T45254 T45221 punct .,exercised
R11611 T45256 T45257 advmod Immediately,following
R11612 T45257 T45258 prep following,subjected
R11613 T45259 T45260 poss its,run
R11614 T45260 T45257 pobj run,following
R11615 T45261 T45260 compound treadmill,run
R11616 T45262 T45258 punct ", ",subjected
R11617 T45263 T45264 det the,mouse
R11618 T45264 T45258 nsubjpass mouse,subjected
R11619 T45265 T45258 auxpass was,subjected
R11620 T45266 T45258 prep to,subjected
R11621 T45267 T45268 amod serial,echocardiography
R11622 T45268 T45266 pobj echocardiography,to
R11623 T45269 T45258 advcl using,subjected
R11624 T45270 T45271 det an,System
R11625 T45271 T45269 dobj System,using
R11626 T45272 T45273 compound Acuson,Echocardiography
R11627 T45273 T45271 compound Echocardiography,System
R11628 T45274 T45273 compound Sequoia,Echocardiography
R11629 T45275 T45271 acl performed,System
R11630 T45276 T45277 mark as,described
R11631 T45277 T45275 advcl described,performed
R11632 T45278 T45277 advmod previously,described
R11633 T45279 T45280 punct [,51
R11634 T45280 T45258 parataxis 51,subjected
R11635 T45281 T45280 punct ],51
R11636 T45282 T45258 punct .,subjected
R11639 T45734 T45735 advmod In,vivo
R11640 T45735 T45736 amod vivo,studies
R11641 T45737 T45736 amod cardiac,studies
R11642 T45738 T45736 amod hemodynamic,studies
R11643 T45740 T45741 amod Hemodynamic,studies
R11644 T45741 T45742 nsubjpass studies,performed
R11645 T45743 T45742 auxpass were,performed
R11646 T45744 T45745 mark as,described
R11647 T45745 T45742 advcl described,performed
R11648 T45746 T45745 advmod previously,described
R11649 T45747 T45745 prep with,described
R11650 T45748 T45749 det some,modifications
R11651 T45749 T45747 pobj modifications,with
R11652 T45750 T45751 punct [,52
R11653 T45751 T45745 parataxis 52,described
R11654 T45752 T45751 punct ],52
R11655 T45753 T45742 punct .,performed
R11656 T45755 T45756 prep In,anesthetized
R11657 T45757 T45755 amod brief,In
R11658 T45758 T45756 punct ", ",anesthetized
R11659 T45759 T45760 amod adult,mice
R11660 T45760 T45756 nsubjpass mice,anesthetized
R11661 T45761 T45762 punct (,wk
R11662 T45762 T45760 parataxis wk,mice
R11663 T45763 T45764 quantmod 10,12
R11664 T45764 T45762 nummod 12,wk
R11665 T45765 T45764 punct –,12
R11666 T45766 T45762 punct ),wk
R11667 T45767 T45756 auxpass were,anesthetized
R11668 T45768 T45756 advmod intraperitoneally,anesthetized
R11669 T45769 T45770 punct (,IP
R11670 T45770 T45768 parataxis IP,intraperitoneally
R11671 T45771 T45770 punct ),IP
R11672 T45772 T45756 prep with,anesthetized
R11673 T45773 T45774 compound thiopental,sodium
R11674 T45774 T45772 pobj sodium,with
R11675 T45775 T45776 punct (,mg
R11676 T45776 T45774 parataxis mg,sodium
R11677 T45777 T45776 nummod 60,mg
R11678 T45778 T45779 punct /,kg
R11679 T45779 T45776 prep kg,mg
R11680 T45780 T45776 punct ),mg
R11681 T45781 T45756 punct .,anesthetized
R11682 T45783 T45784 det The,mice
R11683 T45784 T45785 nsubjpass mice,intubated
R11684 T45786 T45785 auxpass were,intubated
R11685 T45787 T45785 cc and,intubated
R11686 T45788 T45785 conj ventilated,intubated
R11687 T45789 T45788 prep with,ventilated
R11688 T45790 T45791 det a,ventilator
R11689 T45791 T45789 pobj ventilator,with
R11690 T45792 T45791 compound Harvard,ventilator
R11691 T45793 T45785 punct .,intubated
R11692 T45795 T45796 det The,artery
R11693 T45796 T45799 nsubjpass artery,isolated
R11694 T45797 T45796 amod right,artery
R11695 T45798 T45796 compound carotid,artery
R11696 T45800 T45799 auxpass was,isolated
R11697 T45801 T45799 prep in,isolated
R11698 T45802 T45803 det the,region
R11699 T45803 T45801 pobj region,in
R11700 T45804 T45803 prep of,region
R11701 T45805 T45806 det the,trachea
R11702 T45806 T45804 pobj trachea,of
R11703 T45807 T45799 cc and,isolated
R11704 T45808 T45799 conj cannulated,isolated
R11705 T45809 T45808 prep with,cannulated
R11706 T45810 T45811 det a,catheter
R11707 T45811 T45809 pobj catheter,with
R11708 T45812 T45813 nummod 1.4,French
R11709 T45813 T45811 nmod French,catheter
R11710 T45814 T45813 punct -,French
R11711 T45815 T45816 amod high,fidelity
R11712 T45816 T45811 compound fidelity,catheter
R11713 T45817 T45816 punct -,fidelity
R11714 T45818 T45811 compound micromanometer,catheter
R11715 T45819 T45820 punct (,Instruments
R11716 T45820 T45811 parataxis Instruments,catheter
R11717 T45821 T45820 compound Millar,Instruments
R11718 T45822 T45820 punct ", ",Instruments
R11719 T45823 T45820 npadvmod Houston,Instruments
R11720 T45824 T45820 punct ", ",Instruments
R11721 T45825 T45820 npadvmod Texas,Instruments
R11722 T45826 T45820 punct ", ",Instruments
R11723 T45827 T45828 compound United,States
R11724 T45828 T45820 npadvmod States,Instruments
R11725 T45829 T45820 punct ),Instruments
R11726 T45830 T45811 punct ", ",catheter
R11727 T45831 T45832 dep which,inserted
R11728 T45832 T45811 relcl inserted,catheter
R11729 T45833 T45832 auxpass was,inserted
R11730 T45834 T45832 prep into,inserted
R11731 T45835 T45836 det the,ventricle
R11732 T45836 T45834 pobj ventricle,into
R11733 T45837 T45836 amod left,ventricle
R11734 T45838 T45832 advmod retrograde,inserted
R11735 T45839 T45838 prep across,retrograde
R11736 T45840 T45841 det the,valve
R11737 T45841 T45839 pobj valve,across
R11738 T45842 T45841 amod aortic,valve
R11739 T45843 T45799 punct .,isolated
R11740 T45845 T45846 amod Hemodynamic,measurements
R11741 T45846 T45847 nsubjpass measurements,recorded
R11742 T45848 T45847 auxpass were,recorded
R11743 T45849 T45847 prep at,recorded
R11744 T45850 T45849 pobj baseline,at
R11745 T45851 T45849 cc and,at
R11746 T45852 T45853 nummod 3,min
R11747 T45853 T45854 npadvmod min,following
R11748 T45854 T45849 conj following,at
R11749 T45855 T45856 amod continuous,infusion
R11750 T45856 T45854 pobj infusion,following
R11751 T45857 T45856 prep of,infusion
R11752 T45858 T45859 amod incremental,doses
R11753 T45859 T45857 pobj doses,of
R11754 T45860 T45859 prep of,doses
R11755 T45861 T45860 pobj dobutamine,of
R11756 T45862 T45861 punct (,dobutamine
R11757 T45863 T45864 npadvmod β1,adrenergic
R11758 T45864 T45871 amod adrenergic,agonist
R11759 T45865 T45863 punct ", ",β1
R11760 T45866 T45863 conj β2,β1
R11761 T45867 T45866 punct ", ",β2
R11762 T45868 T45866 cc and,β2
R11763 T45869 T45866 conj α1,β2
R11764 T45870 T45864 punct -,adrenergic
R11765 T45871 T45861 appos agonist,dobutamine
R11766 T45872 T45859 punct ),doses
R11767 T45873 T45859 prep up,doses
R11768 T45874 T45873 prep to,up
R11769 T45875 T45876 nummod 32,ng
R11770 T45876 T45874 pobj ng,to
R11771 T45877 T45876 punct ·,ng
R11772 T45878 T45876 appos gBW,ng
R11773 T45879 T45880 punct −,1
R11774 T45880 T45878 nummod 1,gBW
R11775 T45881 T45876 punct ·,ng
R11776 T45882 T45876 appos min,ng
R11777 T45883 T45884 punct −,1
R11778 T45884 T45882 nummod 1,min
R11779 T45885 T45886 punct [,53
R11780 T45886 T45847 parataxis 53,recorded
R11781 T45887 T45886 punct ],53
R11782 T45888 T45847 punct .,recorded
R11783 T45890 T45891 amod Continuous,data
R11784 T45891 T45895 nsubjpass data,acquired
R11785 T45892 T45893 compound pressure,volume
R11786 T45893 T45891 compound volume,data
R11787 T45894 T45893 punct -,volume
R11788 T45896 T45895 auxpass were,acquired
R11789 T45897 T45895 cc and,acquired
R11790 T45898 T45895 conj digitized,acquired
R11791 T45899 T45898 prep with,digitized
R11792 T45900 T45901 det the,system
R11793 T45901 T45899 pobj system,with
R11794 T45902 T45903 compound BioBench,software
R11795 T45903 T45901 compound software,system
R11796 T45904 T45903 compound computer,software
R11797 T45905 T45906 compound data,acquisition
R11798 T45906 T45901 compound acquisition,system
R11799 T45907 T45908 punct (,Instruments
R11800 T45908 T45901 parataxis Instruments,system
R11801 T45909 T45908 compound National,Instruments
R11802 T45910 T45908 punct ", ",Instruments
R11803 T45911 T45908 npadvmod Austin,Instruments
R11804 T45912 T45908 punct ", ",Instruments
R11805 T45913 T45908 npadvmod Texas,Instruments
R11806 T45914 T45908 punct ", ",Instruments
R11807 T45915 T45916 compound United,States
R11808 T45916 T45908 npadvmod States,Instruments
R11809 T45917 T45908 punct ),Instruments
R11810 T45918 T45895 punct .,acquired
R11811 T46753 T46754 amod Isolated,perfusion
R11812 T46755 T46754 amod working,perfusion
R11813 T46756 T46757 compound mouse,heart
R11814 T46757 T46754 compound heart,perfusion
R11815 T46759 T46760 amod Isolated,perfusion
R11816 T46760 T46764 nsubjpass perfusion,based
R11817 T46761 T46760 amod working,perfusion
R11818 T46762 T46763 compound mouse,heart
R11819 T46763 T46760 compound heart,perfusion
R11820 T46765 T46764 auxpass was,based
R11821 T46766 T46764 prep on,based
R11822 T46767 T46768 det a,procedure
R11823 T46768 T46766 pobj procedure,on
R11824 T46769 T46770 advmod previously,described
R11825 T46770 T46768 amod described,procedure
R11826 T46771 T46772 punct [,54
R11827 T46772 T46768 parataxis 54,procedure
R11828 T46773 T46772 punct ],54
R11829 T46774 T46764 punct .,based
R11830 T46776 T46777 amod Adult,mice
R11831 T46777 T46778 nsubjpass mice,heparinized
R11832 T46779 T46780 punct (,old
R11833 T46780 T46777 parataxis old,mice
R11834 T46781 T46782 quantmod 4,7
R11835 T46782 T46784 nummod 7,mo
R11836 T46783 T46782 punct –,7
R11837 T46784 T46780 npadvmod mo,old
R11838 T46785 T46780 punct ),old
R11839 T46786 T46778 auxpass were,heparinized
R11840 T46787 T46788 punct (,units
R11841 T46788 T46778 parataxis units,heparinized
R11842 T46789 T46788 nummod 100,units
R11843 T46790 T46788 advmod IP,units
R11844 T46791 T46788 punct ),units
R11845 T46792 T46793 nummod 10,min
R11846 T46793 T46794 npadvmod min,to
R11847 T46794 T46778 prep to,heparinized
R11848 T46795 T46794 amod prior,to
R11849 T46796 T46794 pobj anesthesia,to
R11850 T46797 T46778 punct .,heparinized
R11851 T46799 T46800 nsubjpass Animals,anesthetized
R11852 T46801 T46800 auxpass were,anesthetized
R11853 T46802 T46800 advmod then,anesthetized
R11854 T46803 T46800 advmod deeply,anesthetized
R11855 T46804 T46800 prep with,anesthetized
R11856 T46805 T46806 quantmod 5,10
R11857 T46806 T46808 nummod 10,mg
R11858 T46807 T46806 punct –,10
R11859 T46808 T46804 pobj mg,with
R11860 T46809 T46808 prep of,mg
R11861 T46810 T46811 compound sodium,pentobarbital
R11862 T46811 T46809 pobj pentobarbital,of
R11863 T46812 T46800 advmod IP,anesthetized
R11864 T46813 T46800 punct .,anesthetized
R11865 T46815 T46816 nsubjpass Hearts,excised
R11866 T46817 T46816 auxpass were,excised
R11867 T46818 T46816 cc and,excised
R11868 T46819 T46816 conj placed,excised
R11869 T46820 T46819 prep in,placed
R11870 T46821 T46822 det an,solution
R11871 T46822 T46820 pobj solution,in
R11872 T46823 T46824 npadvmod ice,cold
R11873 T46824 T46822 amod cold,solution
R11874 T46825 T46824 punct -,cold
R11875 T46826 T46827 nmod Krebs,Henseleit
R11876 T46827 T46829 nmod Henseleit,bicarbonate
R11877 T46828 T46827 punct -,Henseleit
R11878 T46829 T46822 nmod bicarbonate,solution
R11879 T46830 T46829 punct (,bicarbonate
R11880 T46831 T46829 appos KHB,bicarbonate
R11881 T46832 T46822 punct ),solution
R11882 T46833 T46822 punct [,solution
R11883 T46834 T46835 nummod 118,mM
R11884 T46835 T46836 compound mM,NaCl
R11885 T46836 T46822 appos NaCl,solution
R11886 T46837 T46836 punct ", ",NaCl
R11887 T46838 T46839 nummod 25,mM
R11888 T46839 T46840 compound mM,NaHCO3
R11889 T46840 T46836 conj NaHCO3,NaCl
R11890 T46841 T46840 punct ", ",NaHCO3
R11891 T46842 T46843 nummod 4.7,mM
R11892 T46843 T46844 compound mM,KCl
R11893 T46844 T46840 conj KCl,NaHCO3
R11894 T46845 T46844 punct ", ",KCl
R11895 T46846 T46847 nummod 1.2,mM
R11896 T46847 T46848 compound mM,KH2PO4
R11897 T46848 T46844 conj KH2PO4,KCl
R11898 T46849 T46848 punct ", ",KH2PO4
R11899 T46850 T46851 nummod 2.5,mM
R11900 T46851 T46852 compound mM,CaCl2
R11901 T46852 T46848 conj CaCl2,KH2PO4
R11902 T46853 T46852 punct ", ",CaCl2
R11903 T46854 T46855 nummod 5.0,mM
R11904 T46855 T46856 compound mM,glucose
R11905 T46856 T46852 conj glucose,CaCl2
R11906 T46857 T46856 punct ", ",glucose
R11907 T46858 T46856 cc and,glucose
R11908 T46859 T46860 nummod 100,units
R11909 T46860 T46856 conj units,glucose
R11910 T46861 T46862 punct /,insulin
R11911 T46862 T46860 prep insulin,units
R11912 T46863 T46862 compound L,insulin
R11913 T46864 T46865 punct (,pH
R11914 T46865 T46860 parataxis pH,units
R11915 T46866 T46865 nummod 7.4,pH
R11916 T46867 T46865 punct ),pH
R11917 T46868 T46816 punct ],excised
R11918 T46869 T46816 punct .,excised
R11919 T46871 T46872 nsubjpass Hearts,cannulated
R11920 T46873 T46872 auxpass were,cannulated
R11921 T46874 T46872 advmod first,cannulated
R11922 T46875 T46872 prep via,cannulated
R11923 T46876 T46877 det the,aorta
R11924 T46877 T46875 pobj aorta,via
R11925 T46878 T46872 cc and,cannulated
R11926 T46879 T46872 conj perfused,cannulated
R11927 T46880 T46879 advmod retrogradely,perfused
R11928 T46881 T46879 prep by,perfused
R11929 T46882 T46883 det the,method
R11930 T46883 T46881 pobj method,by
R11931 T46884 T46883 compound Langendorff,method
R11932 T46885 T46872 punct .,cannulated
R11933 T46887 T46888 prep Following,switched
R11934 T46889 T46890 amod left,cannulation
R11935 T46890 T46887 pobj cannulation,Following
R11936 T46891 T46890 amod atrial,cannulation
R11937 T46892 T46888 punct ", ",switched
R11938 T46893 T46888 nsubjpass perfusion,switched
R11939 T46894 T46888 auxpass was,switched
R11940 T46895 T46888 prep to,switched
R11941 T46896 T46897 det the,mode
R11942 T46897 T46895 pobj mode,to
R11943 T46898 T46897 amod working,mode
R11944 T46899 T46900 mark with,bound
R11945 T46900 T46888 advcl bound,switched
R11946 T46901 T46902 compound KHB,solution
R11947 T46902 T46900 nsubj solution,bound
R11948 T46903 T46902 acl containing,solution
R11949 T46904 T46905 nummod 1.2,mM
R11950 T46905 T46906 compound mM,palmitate
R11951 T46906 T46903 dobj palmitate,containing
R11952 T46907 T46900 prep to,bound
R11953 T46908 T46909 nummod 3,%
R11954 T46909 T46910 nmod %,BSA
R11955 T46910 T46907 pobj BSA,to
R11956 T46911 T46912 amod fatty,acid
R11957 T46912 T46913 npadvmod acid,free
R11958 T46913 T46910 amod free,BSA
R11959 T46914 T46913 punct -,free
R11960 T46915 T46900 prep with,bound
R11961 T46916 T46917 det a,pressure
R11962 T46917 T46915 pobj pressure,with
R11963 T46918 T46917 amod preload,pressure
R11964 T46919 T46917 prep of,pressure
R11965 T46920 T46921 nummod 11.5,mm
R11966 T46921 T46922 compound mm,Hg
R11967 T46922 T46919 pobj Hg,of
R11968 T46923 T46917 cc and,pressure
R11969 T46924 T46925 det an,pressure
R11970 T46925 T46917 conj pressure,pressure
R11971 T46926 T46925 amod afterload,pressure
R11972 T46927 T46925 prep of,pressure
R11973 T46928 T46929 nummod 50,mm
R11974 T46929 T46930 compound mm,Hg
R11975 T46930 T46927 pobj Hg,of
R11976 T46931 T46900 prep for,bound
R11977 T46932 T46933 nummod 60,min
R11978 T46933 T46931 pobj min,for
R11979 T46934 T46900 prep with,bound
R11980 T46935 T46936 amod oxygenated,solution
R11981 T46936 T46934 pobj solution,with
R11982 T46937 T46936 compound buffer,solution
R11983 T46938 T46888 punct .,switched
R11984 T46940 T46941 amod Functional,measurements
R11985 T46941 T46942 nsubjpass measurements,acquired
R11986 T46943 T46941 punct ", ",measurements
R11987 T46944 T46945 advmod namely,output
R11988 T46945 T46941 appos output,measurements
R11989 T46946 T46945 amod cardiac,output
R11990 T46947 T46945 punct ", ",output
R11991 T46948 T46949 amod aortic,flows
R11992 T46949 T46945 conj flows,output
R11993 T46950 T46949 punct ", ",flows
R11994 T46951 T46952 amod peak,pressure
R11995 T46952 T46949 conj pressure,flows
R11996 T46953 T46952 amod systolic,pressure
R11997 T46954 T46952 punct ", ",pressure
R11998 T46955 T46952 cc and,pressure
R11999 T46956 T46957 compound heart,rate
R12000 T46957 T46952 conj rate,pressure
R12001 T46958 T46942 auxpass were,acquired
R12002 T46959 T46960 det every,min
R12003 T46960 T46942 npadvmod min,acquired
R12004 T46961 T46960 nummod 10,min
R12005 T46962 T46942 advcl using,acquired
R12006 T46963 T46964 compound inline,probes
R12007 T46964 T46962 dobj probes,using
R12008 T46965 T46964 compound flow,probes
R12009 T46966 T46967 punct (,Systems
R12010 T46967 T46964 parataxis Systems,probes
R12011 T46968 T46967 compound Transonic,Systems
R12012 T46969 T46967 punct ", ",Systems
R12013 T46970 T46967 npadvmod Ithaca,Systems
R12014 T46971 T46967 punct ", ",Systems
R12015 T46972 T46973 compound New,York
R12016 T46973 T46967 npadvmod York,Systems
R12017 T46974 T46967 punct ", ",Systems
R12018 T46975 T46976 compound United,States
R12019 T46976 T46967 npadvmod States,Systems
R12020 T46977 T46967 punct ),Systems
R12021 T46978 T46964 punct ", ",probes
R12022 T46979 T46980 det a,transducer
R12023 T46980 T46964 conj transducer,probes
R12024 T46981 T46980 compound pressure,transducer
R12025 T46982 T46983 punct (,104A
R12026 T46983 T46980 parataxis 104A,transducer
R12027 T46984 T46983 compound TSD,104A
R12028 T46985 T46983 punct ", ",104A
R12029 T46986 T46987 compound BIOPAC,Systems
R12030 T46987 T46983 npadvmod Systems,104A
R12031 T46988 T46983 punct ", ",104A
R12032 T46989 T46990 compound Santa,Barbara
R12033 T46990 T46983 npadvmod Barbara,104A
R12034 T46991 T46983 punct ", ",104A
R12035 T46992 T46983 npadvmod California,104A
R12036 T46993 T46983 punct ", ",104A
R12037 T46994 T46995 compound United,States
R12038 T46995 T46983 npadvmod States,104A
R12039 T46996 T46983 punct ),104A
R12040 T46997 T46980 cc and,transducer
R12041 T46998 T46980 conj data,transducer
R12042 T46999 T46998 acl acquired,data
R12043 T47000 T46999 prep with,acquired
R12044 T47001 T47002 det the,system
R12045 T47002 T47000 pobj system,with
R12046 T47003 T47002 compound MP100,system
R12047 T47004 T47002 prep from,system
R12048 T47005 T47004 pobj AcqKnowledge,from
R12049 T47006 T47007 punct (,Systems
R12050 T47007 T47005 parataxis Systems,AcqKnowledge
R12051 T47008 T47007 compound BIOPAC,Systems
R12052 T47009 T47007 punct ),Systems
R12053 T47010 T46942 punct .,acquired
R12054 T47012 T47013 amod Cardiac,work
R12055 T47013 T47014 nsubjpass work,calculated
R12056 T47015 T47014 auxpass was,calculated
R12057 T47016 T47014 prep as,calculated
R12058 T47017 T47018 det the,product
R12059 T47018 T47016 pobj product,as
R12060 T47019 T47018 prep of,product
R12061 T47020 T47021 nmod peak,pressure
R12062 T47021 T47019 pobj pressure,of
R12063 T47022 T47021 amod systolic,pressure
R12064 T47023 T47021 cc and,pressure
R12065 T47024 T47025 amod cardiac,output
R12066 T47025 T47021 conj output,pressure
R12067 T47026 T47014 punct .,calculated
R12068 T47484 T47485 nsubjpass Data,analyzed
R12069 T47486 T47485 auxpass were,analyzed
R12070 T47487 T47485 advcl using,analyzed
R12071 T47488 T47489 compound T,tests
R12072 T47489 T47487 dobj tests,using
R12073 T47490 T47489 punct -,tests
R12074 T47491 T47489 cc or,tests
R12075 T47492 T47489 conj ANOVAs,tests
R12076 T47493 T47494 punct (,measures
R12077 T47494 T47492 parataxis measures,ANOVAs
R12078 T47495 T47494 prep of,measures
R12079 T47496 T47497 amod general,activity
R12080 T47497 T47495 pobj activity,of
R12081 T47498 T47497 cc and,activity
R12082 T47499 T47500 compound sensorimotor,battery
R12083 T47500 T47497 conj battery,activity
R12084 T47501 T47494 punct ),measures
R12085 T47502 T47485 punct .,analyzed
R12086 T47504 T47505 det The,level
R12087 T47505 T47506 nsubjpass level,set
R12088 T47507 T47505 prep of,level
R12089 T47508 T47507 pobj significance,of
R12090 T47509 T47506 auxpass was,set
R12091 T47510 T47506 prep at,set
R12092 T47511 T47510 pobj p,at
R12093 T47512 T47513 punct <,0.05
R12094 T47513 T47511 amod 0.05,p
R12095 T47514 T47506 prep in,set
R12096 T47515 T47516 det all,cases
R12097 T47516 T47514 pobj cases,in
R12098 T47517 T47506 punct .,set
R12099 T47519 T47520 nsubjpass Data,reported
R12100 T47521 T47520 auxpass are,reported
R12101 T47522 T47520 prep as,reported
R12102 T47523 T47524 compound mean,values
R12103 T47524 T47522 pobj values,as
R12104 T47525 T47524 punct ±,values
R12105 T47526 T47524 appos SEM,values
R12106 T47527 T47520 punct ", ",reported
R12107 T47528 T47529 mark unless,noted
R12108 T47529 T47520 advcl noted,reported
R12109 T47530 T47529 advmod otherwise,noted
R12110 T47531 T47520 punct .,reported
R12111 T47533 T47534 det The,model
R12112 T47534 T47536 nsubj model,included
R12113 T47535 T47534 compound ANOVA,model
R12114 T47537 T47534 acl used,model
R12115 T47538 T47539 aux to,analyze
R12116 T47539 T47537 advcl analyze,used
R12117 T47540 T47541 det each,test
R12118 T47541 T47539 dobj test,analyze
R12119 T47542 T47541 compound sensorimotor,test
R12120 T47543 T47544 nummod one,between
R12121 T47544 T47536 dobj between,included
R12122 T47545 T47544 punct -,between
R12123 T47546 T47544 pobj subjects,between
R12124 T47547 T47544 amod variable,between
R12125 T47548 T47549 punct (,genotype
R12126 T47549 T47544 parataxis genotype,between
R12127 T47550 T47549 punct ),genotype
R12128 T47551 T47544 punct ", ",between
R12129 T47552 T47544 cc and,between
R12130 T47553 T47554 nummod one,within
R12131 T47554 T47544 conj within,between
R12132 T47555 T47554 punct -,within
R12133 T47556 T47554 pobj subjects,within
R12134 T47557 T47554 amod variable,within
R12135 T47558 T47559 punct (,trials
R12136 T47559 T47554 parataxis trials,within
R12137 T47560 T47559 punct ),trials
R12138 T47561 T47536 punct .,included
R12139 T47563 T47564 advmod When,conducted
R12140 T47564 T47570 advcl conducted,used
R12141 T47565 T47564 nsubjpass ANOVAs,conducted
R12142 T47566 T47565 prep with,ANOVAs
R12143 T47567 T47568 amod repeated,measures
R12144 T47568 T47566 pobj measures,with
R12145 T47569 T47564 auxpass were,conducted
R12146 T47571 T47570 punct ", ",used
R12147 T47572 T47573 det the,adjustment
R12148 T47573 T47570 nsubjpass adjustment,used
R12149 T47574 T47575 nmod Huynh,Feldt
R12150 T47575 T47573 nmod Feldt,adjustment
R12151 T47576 T47575 punct -,Feldt
R12152 T47577 T47575 punct (,Feldt
R12153 T47578 T47579 compound H,F
R12154 T47579 T47575 appos F,Feldt
R12155 T47580 T47579 punct -,F
R12156 T47581 T47573 punct ),adjustment
R12157 T47582 T47573 prep of,adjustment
R12158 T47583 T47584 compound alpha,levels
R12159 T47584 T47582 pobj levels,of
R12160 T47585 T47570 auxpass was,used
R12161 T47586 T47570 prep for,used
R12162 T47587 T47588 det all,effects
R12163 T47588 T47586 pobj effects,for
R12164 T47589 T47588 nmod within,effects
R12165 T47590 T47589 punct -,within
R12166 T47591 T47589 pobj subjects,within
R12167 T47592 T47588 acl containing,effects
R12168 T47593 T47594 amod more,two
R12169 T47594 T47596 nummod two,levels
R12170 T47595 T47594 quantmod than,two
R12171 T47596 T47592 dobj levels,containing
R12172 T47597 T47570 punct ", ",used
R12173 T47598 T47570 prep in,used
R12174 T47599 T47598 pobj order,in
R12175 T47600 T47601 aux to,protect
R12176 T47601 T47599 acl protect,order
R12177 T47602 T47601 prep against,protect
R12178 T47603 T47602 pobj violations,against
R12179 T47604 T47603 prep of,violations
R12180 T47605 T47606 det the,assumptions
R12181 T47606 T47604 pobj assumptions,of
R12182 T47607 T47606 nmod sphericity,assumptions
R12183 T47608 T47607 punct /,sphericity
R12184 T47609 T47610 amod compound,symmetry
R12185 T47610 T47607 appos symmetry,sphericity
R12186 T47611 T47606 acl underlying,assumptions
R12187 T47612 T47613 det this,model
R12188 T47613 T47611 dobj model,underlying
R12189 T47614 T47613 compound ANOVA,model
R12190 T47615 T47570 punct .,used
R12191 T47617 T47618 prep In,used
R12192 T47619 T47617 pobj addition,In
R12193 T47620 T47618 punct ", ",used
R12194 T47621 T47622 compound Bonferroni,correction
R12195 T47622 T47618 nsubjpass correction,used
R12196 T47623 T47618 auxpass was,used
R12197 T47624 T47625 advmod when,appropriate
R12198 T47625 T47618 advcl appropriate,used
R12199 T47626 T47627 aux to,help
R12200 T47627 T47618 advcl help,used
R12201 T47628 T47627 xcomp maintain,help
R12202 T47629 T47630 amod prescribed,levels
R12203 T47630 T47628 dobj levels,maintain
R12204 T47631 T47630 compound alpha,levels
R12205 T47632 T47633 punct (,p
R12206 T47633 T47630 parataxis p,levels
R12207 T47634 T47633 advmod e.g.,p
R12208 T47635 T47633 punct ", ",p
R12209 T47636 T47637 punct <,0.05
R12210 T47637 T47633 amod 0.05,p
R12211 T47638 T47633 punct ),p
R12212 T47639 T47640 advmod when,conducted
R12213 T47640 T47628 advcl conducted,maintain
R12214 T47641 T47642 amod multiple,comparisons
R12215 T47642 T47640 nsubjpass comparisons,conducted
R12216 T47643 T47640 auxpass were,conducted
R12217 T47644 T47618 punct .,used
R8687 T34069 T34070 det the,gene
R8688 T34070 T34068 dobj gene,Targeting
R8689 T34071 T34072 compound PGC,1α
R8690 T34072 T34070 compound 1α,gene
R8691 T34073 T34072 punct -,1α
R8692 T34074 T34068 prep in,Targeting
R8693 T34075 T34074 pobj mice,in
R8694 T34077 T34078 det A,clone
R8695 T34078 T34081 nsubjpass clone,obtained
R8696 T34079 T34078 nmod BAC,clone
R8697 T34080 T34078 amod genomic,clone
R8698 T34082 T34078 acl containing,clone
R8699 T34083 T34084 det the,gene
R8700 T34084 T34082 dobj gene,containing
R8701 T34085 T34084 amod murine,gene
R8702 T34086 T34087 compound PGC,1α
R8703 T34087 T34084 compound 1α,gene
R8704 T34088 T34087 punct -,1α
R8705 T34089 T34084 punct ", ",gene
R8706 T34090 T34084 acl isolated,gene
R8707 T34091 T34090 prep from,isolated
R8708 T34092 T34093 det an,library
R8709 T34093 T34091 pobj library,from
R8710 T34094 T34093 nmod Sv129,library
R8711 T34095 T34093 amod genomic,library
R8712 T34096 T34081 punct ", ",obtained
R8713 T34097 T34081 auxpass was,obtained
R8714 T34098 T34081 prep from,obtained
R8715 T34099 T34100 compound Incyte,Genomics
R8716 T34100 T34098 pobj Genomics,from
R8717 T34101 T34102 punct (,Alto
R8718 T34102 T34100 parataxis Alto,Genomics
R8719 T34103 T34102 compound Palo,Alto
R8720 T34104 T34102 punct ", ",Alto
R8721 T34105 T34102 npadvmod California,Alto
R8722 T34106 T34102 punct ", ",Alto
R8723 T34107 T34108 compound United,States
R8724 T34108 T34102 npadvmod States,Alto
R8725 T34109 T34102 punct ),Alto
R8726 T34110 T34081 punct .,obtained
R8727 T34112 T34113 det A,region
R8728 T34113 T34117 nsubjpass region,amplified
R8729 T34114 T34115 nummod 3,kb
R8730 T34115 T34113 compound kb,region
R8731 T34116 T34115 punct -,kb
R8732 T34118 T34113 acl spanning,region
R8733 T34119 T34118 dobj exon,spanning
R8734 T34120 T34119 nummod 3,exon
R8735 T34121 T34117 auxpass was,amplified
R8736 T34122 T34117 prep from,amplified
R8737 T34123 T34124 det the,clone
R8738 T34124 T34122 pobj clone,from
R8739 T34125 T34124 amod genomic,clone
R8740 T34126 T34117 punct .,amplified
R8741 T34128 T34129 det A,primer
R8742 T34129 T34132 nsubjpass primer,designed
R8743 T34130 T34129 nummod 5,primer
R8744 T34131 T34130 punct ′,5
R8745 T34133 T34132 auxpass was,designed
R8746 T34134 T34135 aux to,amplify
R8747 T34135 T34132 advcl amplify,designed
R8748 T34136 T34137 det a,fragment
R8749 T34137 T34135 dobj fragment,amplify
R8750 T34138 T34137 prep with,fragment
R8751 T34139 T34140 det the,end
R8752 T34140 T34143 nsubj end,beginning
R8753 T34141 T34140 nummod 5,end
R8754 T34142 T34141 punct ′,5
R8755 T34143 T34138 pcomp beginning,with
R8756 T34144 T34145 nummod 732,bp
R8757 T34145 T34146 npadvmod bp,upstream
R8758 T34146 T34143 advmod upstream,beginning
R8759 T34147 T34146 prep of,upstream
R8760 T34148 T34147 pobj exon,of
R8761 T34149 T34148 nummod 3,exon
R8762 T34150 T34151 advmod just,upstream
R8763 T34151 T34143 advmod upstream,beginning
R8764 T34152 T34151 prep of,upstream
R8765 T34153 T34154 det an,site
R8766 T34154 T34152 pobj site,of
R8767 T34155 T34154 amod endogenous,site
R8768 T34156 T34154 compound Kpn1,site
R8769 T34157 T34154 compound restriction,site
R8770 T34158 T34159 punct (,AGTTTCCTTAGCAACTTCATA
R8771 T34159 T34154 parataxis AGTTTCCTTAGCAACTTCATA,site
R8772 T34160 T34159 nummod 5,AGTTTCCTTAGCAACTTCATA
R8773 T34161 T34160 punct ′,5
R8774 T34162 T34159 punct -,AGTTTCCTTAGCAACTTCATA
R8775 T34163 T34159 punct -,AGTTTCCTTAGCAACTTCATA
R8776 T34164 T34159 nummod 3,AGTTTCCTTAGCAACTTCATA
R8777 T34165 T34159 punct ′,AGTTTCCTTAGCAACTTCATA
R8778 T34166 T34159 punct ),AGTTTCCTTAGCAACTTCATA
R8779 T34167 T34132 punct .,designed
R8780 T34169 T34170 det The,primer
R8781 T34170 T34173 nsubj primer,contained
R8782 T34171 T34170 nummod 3,primer
R8783 T34172 T34171 punct ′,3
R8784 T34174 T34175 det a,site
R8785 T34175 T34173 dobj site,contained
R8786 T34176 T34175 compound BamH1,site
R8787 T34177 T34175 acl engineered,site
R8788 T34178 T34177 prep by,engineered
R8789 T34179 T34178 pcomp mutating,by
R8790 T34180 T34181 det the,bases
R8791 T34181 T34179 dobj bases,mutating
R8792 T34182 T34181 acl shown,bases
R8793 T34183 T34182 prep in,shown
R8794 T34184 T34183 pobj lowercase,in
R8795 T34185 T34186 punct (,AAGGATTTTAgGATcc CAGTAC
R8796 T34186 T34175 parataxis AAGGATTTTAgGATcc CAGTAC,site
R8797 T34187 T34186 nummod 5,AAGGATTTTAgGATcc CAGTAC
R8798 T34188 T34187 punct ′,5
R8799 T34189 T34186 punct -,AAGGATTTTAgGATcc CAGTAC
R8800 T34190 T34186 punct -,AAGGATTTTAgGATcc CAGTAC
R8801 T34191 T34186 nummod 3,AAGGATTTTAgGATcc CAGTAC
R8802 T34192 T34186 punct ′,AAGGATTTTAgGATcc CAGTAC
R8803 T34193 T34186 punct ),AAGGATTTTAgGATcc CAGTAC
R8804 T34194 T34173 punct .,contained
R8805 T34196 T34197 det A,fragment
R8806 T34197 T34199 nsubjpass fragment,amplified
R8807 T34198 T34197 amod second,fragment
R8808 T34200 T34197 advmod downstream,fragment
R8809 T34201 T34200 prep of,downstream
R8810 T34202 T34201 pobj exon,of
R8811 T34203 T34202 nummod 5,exon
R8812 T34204 T34199 auxpass was,amplified
R8813 T34205 T34199 punct .,amplified
R8814 T34207 T34208 prep In,engineered
R8815 T34209 T34210 det this,amplicon
R8816 T34210 T34207 pobj amplicon,In
R8817 T34211 T34210 amod latter,amplicon
R8818 T34212 T34208 punct ", ",engineered
R8819 T34213 T34214 nmod Not1,sites
R8820 T34214 T34208 nsubjpass sites,engineered
R8821 T34215 T34213 cc and,Not1
R8822 T34216 T34213 conj Xho1,Not1
R8823 T34217 T34208 auxpass were,engineered
R8824 T34218 T34208 prep into,engineered
R8825 T34219 T34220 det the,5
R8826 T34220 T34221 nummod 5,primers
R8827 T34221 T34218 pobj primers,into
R8828 T34222 T34220 punct ′,5
R8829 T34223 T34220 cc and,5
R8830 T34224 T34220 conj 3,5
R8831 T34225 T34224 punct ′,3
R8832 T34226 T34208 punct ", ",engineered
R8833 T34227 T34208 advmod respectively,engineered
R8834 T34228 T34229 punct (,TGGAGTgc GGCCGCTGGGA
R8835 T34229 T34208 parataxis TGGAGTgc GGCCGCTGGGA,engineered
R8836 T34230 T34229 nummod 5,TGGAGTgc GGCCGCTGGGA
R8837 T34231 T34230 punct ′,5
R8838 T34232 T34229 punct -,TGGAGTgc GGCCGCTGGGA
R8839 T34233 T34229 punct -,TGGAGTgc GGCCGCTGGGA
R8840 T34234 T34229 nummod 3,TGGAGTgc GGCCGCTGGGA
R8841 T34235 T34229 punct ′,TGGAGTgc GGCCGCTGGGA
R8842 T34236 T34229 cc and,TGGAGTgc GGCCGCTGGGA
R8843 T34237 T34238 nummod 5,AAAGAGTCTCgAg AATAGTTTCT
R8844 T34238 T34229 conj AAAGAGTCTCgAg AATAGTTTCT,TGGAGTgc GGCCGCTGGGA
R8845 T34239 T34237 punct ′,5
R8846 T34240 T34238 punct -,AAAGAGTCTCgAg AATAGTTTCT
R8847 T34241 T34238 punct -,AAAGAGTCTCgAg AATAGTTTCT
R8848 T34242 T34238 nummod 3,AAAGAGTCTCgAg AATAGTTTCT
R8849 T34243 T34238 punct ′,AAAGAGTCTCgAg AATAGTTTCT
R8850 T34244 T34229 punct ),TGGAGTgc GGCCGCTGGGA
R8851 T34245 T34208 punct .,engineered
R8852 T34247 T34248 det The,fragments
R8853 T34248 T34249 nsubjpass fragments,cloned
R8854 T34250 T34249 auxpass were,cloned
R8855 T34251 T34249 prep into,cloned
R8856 T34252 T34253 compound p1339,Neomycin
R8857 T34253 T34257 compound Neomycin,vector
R8858 T34254 T34253 punct -,Neomycin
R8859 T34255 T34253 compound PGK,Neomycin
R8860 T34256 T34253 punct -,Neomycin
R8861 T34257 T34251 pobj vector,into
R8862 T34258 T34257 compound targeting,vector
R8863 T34259 T34249 punct .,cloned
R8864 T34261 T34262 det The,construct
R8865 T34262 T34263 nsubjpass construct,linearized
R8866 T34264 T34263 auxpass was,linearized
R8867 T34265 T34263 prep with,linearized
R8868 T34266 T34265 pobj Xho1,with
R8869 T34267 T34263 cc and,linearized
R8870 T34268 T34263 conj electroporated,linearized
R8871 T34269 T34268 prep into,electroporated
R8872 T34270 T34271 compound RW4,cells
R8873 T34271 T34269 pobj cells,into
R8874 T34272 T34271 compound ES,cells
R8875 T34273 T34271 punct (,cells
R8876 T34274 T34275 npadvmod Sv129,derived
R8877 T34275 T34271 amod derived,cells
R8878 T34276 T34268 punct ),electroporated
R8879 T34277 T34268 advcl using,electroporated
R8880 T34278 T34279 compound G418,selection
R8881 T34279 T34277 dobj selection,using
R8882 T34280 T34263 punct .,linearized
R8883 T34282 T34283 det The,electroporation
R8884 T34283 T34284 nsubjpass electroporation,performed
R8885 T34285 T34284 auxpass was,performed
R8886 T34286 T34284 agent by,performed
R8887 T34287 T34288 det the,Core
R8888 T34288 T34286 pobj Core,by
R8889 T34289 T34290 compound Siteman,Center
R8890 T34290 T34288 compound Center,Core
R8891 T34291 T34290 compound Cancer,Center
R8892 T34292 T34293 compound ES,Cell
R8893 T34293 T34288 compound Cell,Core
R8894 T34294 T34288 prep at,Core
R8895 T34295 T34296 compound Washington,University
R8896 T34296 T34294 pobj University,at
R8897 T34297 T34298 punct (,Louis
R8898 T34298 T34296 parataxis Louis,University
R8899 T34299 T34298 compound St.,Louis
R8900 T34300 T34298 punct ", ",Louis
R8901 T34301 T34298 npadvmod Missouri,Louis
R8902 T34302 T34298 punct ", ",Louis
R8903 T34303 T34304 compound United,States
R8904 T34304 T34298 npadvmod States,Louis
R8905 T34305 T34298 punct ),Louis
R8906 T34306 T34284 punct .,performed
R8907 T34308 T34309 det The,clones
R8908 T34309 T34310 nsubjpass clones,screened
R8909 T34311 T34310 auxpass were,screened
R8910 T34312 T34310 prep by,screened
R8911 T34313 T34314 compound Southern,blot
R8912 T34314 T34312 pobj blot,by
R8913 T34315 T34310 advcl using,screened
R8914 T34316 T34317 det an,digest
R8915 T34317 T34315 dobj digest,using
R8916 T34318 T34317 compound Xba1,digest
R8917 T34319 T34320 punct (,see
R8918 T34320 T34310 parataxis see,screened
R8919 T34321 T34322 compound Figure,1A
R8920 T34322 T34320 dobj 1A,see
R8921 T34323 T34322 cc and,1A
R8922 T34324 T34322 conj 1B,1A
R8923 T34325 T34320 punct ),see
R8924 T34326 T34310 punct .,screened
R8925 T34328 T34329 nummod One,clone
R8926 T34329 T34330 nsubj clone,was
R8927 T34331 T34329 prep out,clone
R8928 T34332 T34331 prep of,out
R8929 T34333 T34334 advmod approximately,400
R8930 T34334 T34332 pobj 400,of
R8931 T34335 T34334 acl screened,400
R8932 T34336 T34330 acomp positive,was
R8933 T34337 T34336 prep for,positive
R8934 T34338 T34339 det the,recombination
R8935 T34339 T34337 pobj recombination,for
R8936 T34340 T34339 amod homologous,recombination
R8937 T34341 T34339 prep on,recombination
R8938 T34342 T34343 det the,end
R8939 T34343 T34341 pobj end,on
R8940 T34344 T34343 nummod 3,end
R8941 T34345 T34344 punct ′,3
R8942 T34346 T34339 cc and,recombination
R8943 T34347 T34348 det an,insertion
R8944 T34348 T34339 conj insertion,recombination
R8945 T34349 T34348 prep on,insertion
R8946 T34350 T34351 det the,end
R8947 T34351 T34349 pobj end,on
R8948 T34352 T34351 nummod 5,end
R8949 T34353 T34352 punct ′,5
R8950 T34354 T34330 punct .,was
R8951 T34356 T34357 det This,clone
R8952 T34357 T34358 nsubjpass clone,injected
R8953 T34359 T34358 auxpass was,injected
R8954 T34360 T34358 prep into,injected
R8955 T34361 T34362 det a,blastocyst
R8956 T34362 T34360 pobj blastocyst,into
R8957 T34363 T34364 compound C57BL6,J
R8958 T34364 T34362 compound J,blastocyst
R8959 T34365 T34364 punct /,J
R8960 T34366 T34358 punct .,injected
R8961 T34368 T34369 nsubjpass Chimeras,mated
R8962 T34370 T34369 auxpass were,mated
R8963 T34371 T34369 prep to,mated
R8964 T34372 T34373 compound C57BL6,J
R8965 T34373 T34375 compound J,mice
R8966 T34374 T34373 punct /,J
R8967 T34375 T34371 pobj mice,to
R8968 T34376 T34369 cc and,mated
R8969 T34377 T34378 compound germline,transmission
R8970 T34378 T34379 nsubjpass transmission,confirmed
R8971 T34379 T34369 conj confirmed,mated
R8972 T34380 T34379 auxpass was,confirmed
R8973 T34381 T34379 prep by,confirmed
R8974 T34382 T34383 compound Southern,blotting
R8975 T34383 T34381 pobj blotting,by
R8976 T34384 T34383 prep of,blotting
R8977 T34385 T34386 compound tail,DNA
R8978 T34386 T34384 pobj DNA,of
R8979 T34387 T34388 punct (,see
R8980 T34388 T34379 parataxis see,confirmed
R8981 T34389 T34390 compound Figure,1B
R8982 T34390 T34388 dobj 1B,see
R8983 T34391 T34388 punct ),see
R8984 T34392 T34379 punct .,confirmed
R8985 T34394 T34395 det All,experiments
R8986 T34395 T34396 nsubjpass experiments,performed
R8987 T34397 T34396 auxpass were,performed
R8988 T34398 T34396 advcl using,performed
R8989 T34399 T34400 npadvmod sex,matched
R8990 T34400 T34405 amod matched,controls
R8991 T34401 T34399 punct -,sex
R8992 T34402 T34399 cc and,sex
R8993 T34403 T34399 conj age,sex
R8994 T34404 T34400 punct -,matched
R8995 T34405 T34398 dobj controls,using
R8996 T34406 T34400 cc or,matched
R8997 T34407 T34400 conj littermate,matched
R8998 T34408 T34409 mark as,noted
R8999 T34409 T34396 advcl noted,performed
R9000 T34410 T34396 punct .,performed
R9001 T35414 T35415 amod General,studies
R9002 T35416 T35415 compound animal,studies
R9003 T35418 T35419 det All,experiments
R9004 T35419 T35421 nsubjpass experiments,conducted
R9005 T35420 T35419 compound animal,experiments
R9006 T35422 T35419 cc and,experiments
R9007 T35423 T35424 compound euthanasia,protocols
R9008 T35424 T35419 conj protocols,experiments
R9009 T35425 T35421 auxpass were,conducted
R9010 T35426 T35421 prep in,conducted
R9011 T35427 T35428 amod strict,accordance
R9012 T35428 T35426 pobj accordance,in
R9013 T35429 T35428 prep with,accordance
R9014 T35430 T35431 det the,Institutes
R9015 T35431 T35433 nmod Institutes,guidelines
R9016 T35432 T35431 nmod National,Institutes
R9017 T35433 T35429 pobj guidelines,with
R9018 T35434 T35431 prep of,Institutes
R9019 T35435 T35434 pobj Health,of
R9020 T35436 T35433 prep for,guidelines
R9021 T35437 T35438 compound humane,treatment
R9022 T35438 T35436 pobj treatment,for
R9023 T35439 T35438 prep of,treatment
R9024 T35440 T35439 pobj animals,of
R9025 T35441 T35421 cc and,conducted
R9026 T35442 T35443 auxpass were,reviewed
R9027 T35443 T35421 conj reviewed,conducted
R9028 T35444 T35443 cc and,reviewed
R9029 T35445 T35443 conj approved,reviewed
R9030 T35446 T35445 agent by,approved
R9031 T35447 T35448 det the,Committee
R9032 T35448 T35446 pobj Committee,by
R9033 T35449 T35450 compound Animal,Care
R9034 T35450 T35448 compound Care,Committee
R9035 T35451 T35448 prep of,Committee
R9036 T35452 T35453 compound Washington,University
R9037 T35453 T35451 pobj University,of
R9038 T35454 T35421 punct .,conducted
R9039 T35456 T35457 nsubjpass Animals,weighed
R9040 T35458 T35457 auxpass were,weighed
R9041 T35459 T35457 prep at,weighed
R9042 T35460 T35461 amod different,points
R9043 T35461 T35459 pobj points,at
R9044 T35462 T35461 compound time,points
R9045 T35463 T35457 punct .,weighed
R9046 T35465 T35466 amod Male,mice
R9047 T35466 T35490 nsubjpass mice,euthanized
R9048 T35467 T35465 cc and,Male
R9049 T35468 T35465 conj female,Male
R9050 T35469 T35470 quantmod 3,8
R9051 T35470 T35473 nummod 8,wk
R9052 T35471 T35470 punct -,8
R9053 T35472 T35470 quantmod to,8
R9054 T35473 T35475 npadvmod wk,old
R9055 T35474 T35473 punct -,wk
R9056 T35475 T35466 amod old,mice
R9057 T35476 T35475 punct -,old
R9058 T35477 T35478 nmod PGC,1α
R9059 T35478 T35466 nmod 1α,mice
R9060 T35479 T35478 punct -,1α
R9061 T35480 T35478 punct +,1α
R9062 T35481 T35478 punct /,1α
R9063 T35482 T35478 punct +,1α
R9064 T35483 T35478 cc and,1α
R9065 T35484 T35485 compound PGC,1α
R9066 T35485 T35478 conj 1α,1α
R9067 T35486 T35485 punct -,1α
R9068 T35487 T35485 punct −,1α
R9069 T35488 T35485 punct /,1α
R9070 T35489 T35485 punct −,1α
R9071 T35491 T35490 auxpass were,euthanized
R9072 T35492 T35490 punct ", ",euthanized
R9073 T35493 T35490 cc and,euthanized
R9074 T35494 T35495 nsubjpass tissues,dissected
R9075 T35495 T35490 conj dissected,euthanized
R9076 T35496 T35495 auxpass were,dissected
R9077 T35497 T35495 cc and,dissected
R9078 T35498 T35495 conj weighed,dissected
R9079 T35499 T35498 prep on,weighed
R9080 T35500 T35501 det an,balance
R9081 T35501 T35499 pobj balance,on
R9082 T35502 T35501 amod analytical,balance
R9083 T35503 T35495 punct .,dissected
R9084 T35505 T35506 compound Tissue,weights
R9085 T35506 T35507 nsubjpass weights,corrected
R9086 T35508 T35507 auxpass were,corrected
R9087 T35509 T35507 prep for,corrected
R9088 T35510 T35511 amod total,weight
R9089 T35511 T35509 pobj weight,for
R9090 T35512 T35511 compound body,weight
R9091 T35513 T35507 prep before,corrected
R9092 T35514 T35513 pobj comparison,before
R9093 T35515 T35507 punct .,corrected
R9094 T35517 T35518 compound DEXA,studies
R9095 T35518 T35519 nsubjpass studies,performed
R9096 T35520 T35519 auxpass were,performed
R9097 T35521 T35522 mark as,described
R9098 T35522 T35519 advcl described,performed
R9099 T35523 T35522 advmod previously,described
R9100 T35524 T35525 punct [,39
R9101 T35525 T35522 parataxis 39,described
R9102 T35526 T35525 punct ],39
R9103 T35527 T35519 advcl using,performed
R9104 T35528 T35529 det a,system
R9105 T35529 T35527 dobj system,using
R9106 T35530 T35529 compound Lunar,system
R9107 T35531 T35529 compound PIXIMUS,system
R9108 T35532 T35529 compound DEXA,system
R9109 T35533 T35527 prep at,using
R9110 T35534 T35535 nummod 10,wk
R9111 T35535 T35533 pobj wk,at
R9112 T35536 T35534 punct ", ",10
R9113 T35537 T35534 conj 18,10
R9114 T35538 T35537 punct ", ",18
R9115 T35539 T35537 cc and,18
R9116 T35540 T35537 conj 24,18
R9117 T35541 T35527 prep in,using
R9118 T35542 T35543 amod male,mice
R9119 T35543 T35541 pobj mice,in
R9120 T35544 T35542 cc and,male
R9121 T35545 T35542 conj female,male
R9122 T35546 T35547 nmod PGC,1α
R9123 T35547 T35543 nmod 1α,mice
R9124 T35548 T35547 punct -,1α
R9125 T35549 T35547 punct +,1α
R9126 T35550 T35547 punct /,1α
R9127 T35551 T35547 punct +,1α
R9128 T35552 T35547 cc and,1α
R9129 T35553 T35554 compound PGC,1α
R9130 T35554 T35547 conj 1α,1α
R9131 T35555 T35554 punct -,1α
R9132 T35556 T35554 punct −,1α
R9133 T35557 T35554 punct /,1α
R9134 T35558 T35554 punct −,1α
R9135 T35559 T35519 punct .,performed
R9136 T35561 T35562 prep For,housed
R9137 T35563 T35564 compound cold,experiments
R9138 T35564 T35561 pobj experiments,For
R9139 T35565 T35564 compound exposure,experiments
R9140 T35566 T35562 punct ", ",housed
R9141 T35567 T35568 amod male,mice
R9142 T35568 T35562 nsubjpass mice,housed
R9143 T35569 T35567 cc and,male
R9144 T35570 T35567 conj female,male
R9145 T35571 T35572 nmod PGC,1α
R9146 T35572 T35568 nmod 1α,mice
R9147 T35573 T35572 punct -,1α
R9148 T35574 T35572 punct +,1α
R9149 T35575 T35572 punct /,1α
R9150 T35576 T35572 punct +,1α
R9151 T35577 T35572 cc and,1α
R9152 T35578 T35579 compound PGC,1α
R9153 T35579 T35572 conj 1α,1α
R9154 T35580 T35579 punct -,1α
R9155 T35581 T35579 punct −,1α
R9156 T35582 T35579 punct /,1α
R9157 T35583 T35579 punct −,1α
R9158 T35584 T35562 auxpass were,housed
R9159 T35585 T35562 advmod singly,housed
R9160 T35586 T35562 cc and,housed
R9161 T35587 T35562 conj placed,housed
R9162 T35588 T35587 prep at,placed
R9163 T35589 T35590 nummod 4,°C
R9164 T35590 T35588 pobj °C,at
R9165 T35591 T35587 prep for,placed
R9166 T35592 T35593 nummod 5,h
R9167 T35593 T35591 pobj h,for
R9168 T35594 T35587 prep without,placed
R9169 T35595 T35594 pobj food,without
R9170 T35596 T35562 punct .,housed
R9171 T35598 T35599 compound Core,temperatures
R9172 T35599 T35601 nsubjpass temperatures,monitored
R9173 T35600 T35599 compound body,temperatures
R9174 T35602 T35601 auxpass were,monitored
R9175 T35603 T35601 prep by,monitored
R9176 T35604 T35605 amod rectal,probe
R9177 T35605 T35603 pobj probe,by
R9178 T35606 T35601 prep at,monitored
R9179 T35607 T35606 pobj baseline,at
R9180 T35608 T35607 cc and,baseline
R9181 T35609 T35610 det every,hour
R9182 T35610 T35607 conj hour,baseline
R9183 T35611 T35610 advmod thereafter,hour
R9184 T35612 T35601 punct .,monitored
R9185 T35614 T35615 nsubjpass Mice,monitored
R9186 T35616 T35615 auxpass were,monitored
R9187 T35617 T35618 advmod at,least
R9188 T35618 T35619 advmod least,min
R9189 T35619 T35615 npadvmod min,monitored
R9190 T35620 T35619 det every,min
R9191 T35621 T35619 nummod 30,min
R9192 T35622 T35623 aux to,check
R9193 T35623 T35615 advcl check,monitored
R9194 T35624 T35623 prep for,check
R9195 T35625 T35624 pobj lethargy,for
R9196 T35626 T35615 punct .,monitored
R9197 T35628 T35629 prep At,sacrificed
R9198 T35630 T35631 det the,end
R9199 T35631 T35628 pobj end,At
R9200 T35632 T35631 prep of,end
R9201 T35633 T35634 nummod 5,h
R9202 T35634 T35632 pobj h,of
R9203 T35635 T35629 punct ", ",sacrificed
R9204 T35636 T35629 nsubjpass mice,sacrificed
R9205 T35637 T35629 auxpass were,sacrificed
R9206 T35638 T35629 cc and,sacrificed
R9207 T35639 T35640 nsubj tissues,harvested
R9208 T35640 T35629 conj harvested,sacrificed
R9209 T35641 T35640 prep for,harvested
R9210 T35642 T35643 nmod RNA,extraction
R9211 T35643 T35641 pobj extraction,for
R9212 T35644 T35642 cc and,RNA
R9213 T35645 T35642 conj protein,RNA
R9214 T35646 T35629 punct .,sacrificed
R9215 T35648 T35649 prep For,housed
R9216 T35650 T35651 compound fasting,studies
R9217 T35651 T35648 pobj studies,For
R9218 T35652 T35649 punct ", ",housed
R9219 T35653 T35649 nsubjpass animals,housed
R9220 T35654 T35649 auxpass were,housed
R9221 T35655 T35649 advmod singly,housed
R9222 T35656 T35649 cc and,housed
R9223 T35657 T35649 conj given,housed
R9224 T35658 T35657 dobj water,given
R9225 T35659 T35660 advmod ad,libitum
R9226 T35660 T35657 advmod libitum,given
R9227 T35661 T35649 punct .,housed
R9228 T35663 T35664 nsubjpass Food,removed
R9229 T35665 T35664 auxpass was,removed
R9230 T35666 T35664 prep from,removed
R9231 T35667 T35666 pobj cages,from
R9232 T35668 T35664 prep in,removed
R9233 T35669 T35670 det the,morning
R9234 T35670 T35668 pobj morning,in
R9235 T35671 T35664 cc and,removed
R9236 T35672 T35673 nsubj tissues,harvested
R9237 T35673 T35664 conj harvested,removed
R9238 T35674 T35673 prep at,harvested
R9239 T35675 T35676 nummod 24,h
R9240 T35676 T35674 pobj h,at
R9241 T35677 T35673 prep for,harvested
R9242 T35678 T35677 pobj RNA,for
R9243 T35679 T35678 cc and,RNA
R9244 T35680 T35678 conj histology,RNA
R9245 T35681 T35673 punct .,harvested
R9246 T35683 T35684 nsubjpass Photography,performed
R9247 T35685 T35683 prep of,Photography
R9248 T35686 T35687 det the,mice
R9249 T35687 T35685 pobj mice,of
R9250 T35688 T35684 auxpass was,performed
R9251 T35689 T35684 agent by,performed
R9252 T35690 T35689 pobj MedPic,by
R9253 T35691 T35690 prep at,MedPic
R9254 T35692 T35693 compound Washington,University
R9255 T35693 T35694 compound University,School
R9256 T35694 T35691 pobj School,at
R9257 T35695 T35694 prep of,School
R9258 T35696 T35695 pobj Medicine,of
R9259 T35697 T35684 punct .,performed
R9260 T35699 T35700 nummod 48,h
R9261 T35700 T35702 compound h,monitoring
R9262 T35701 T35700 punct -,h
R9263 T35702 T35704 nsubjpass monitoring,performed
R9264 T35703 T35702 compound activity,monitoring
R9265 T35705 T35704 auxpass was,performed
R9266 T35706 T35704 agent by,performed
R9267 T35707 T35708 compound JAX,Services
R9268 T35708 T35706 pobj Services,by
R9269 T35709 T35710 punct (,Laboratory
R9270 T35710 T35708 parataxis Laboratory,Services
R9271 T35711 T35710 det The,Laboratory
R9272 T35712 T35710 compound Jackson,Laboratory
R9273 T35713 T35710 punct ", ",Laboratory
R9274 T35714 T35715 compound West,Sacramento
R9275 T35715 T35710 npadvmod Sacramento,Laboratory
R9276 T35716 T35710 punct ", ",Laboratory
R9277 T35717 T35710 npadvmod California,Laboratory
R9278 T35718 T35710 punct ", ",Laboratory
R9279 T35719 T35720 compound United,States
R9280 T35720 T35710 npadvmod States,Laboratory
R9281 T35721 T35710 punct ),Laboratory
R9282 T35722 T35704 advcl using,performed
R9283 T35723 T35724 det a,System
R9284 T35724 T35722 dobj System,using
R9285 T35725 T35724 compound Comprehensive,System
R9286 T35726 T35727 compound Laboratory,Animal
R9287 T35727 T35724 compound Animal,System
R9288 T35728 T35724 compound Monitoring,System
R9289 T35729 T35730 punct (,CLAMS
R9290 T35730 T35724 parataxis CLAMS,System
R9291 T35731 T35730 punct ", ",CLAMS
R9292 T35732 T35733 compound Columbus,Instruments
R9293 T35733 T35730 npadvmod Instruments,CLAMS
R9294 T35734 T35730 punct ", ",CLAMS
R9295 T35735 T35730 npadvmod Columbus,CLAMS
R9296 T35736 T35730 punct ", ",CLAMS
R9297 T35737 T35730 npadvmod Ohio,CLAMS
R9298 T35738 T35730 punct ", ",CLAMS
R9299 T35739 T35740 compound United,States
R9300 T35740 T35730 npadvmod States,CLAMS
R9301 T35741 T35730 punct ),CLAMS
R9302 T35742 T35704 punct .,performed
R9303 T35744 T35745 advmod Briefly,acclimated
R9304 T35746 T35745 punct ", ",acclimated
R9305 T35747 T35748 nummod 3,mo
R9306 T35748 T35750 npadvmod mo,old
R9307 T35749 T35748 punct -,mo
R9308 T35750 T35752 amod old,mice
R9309 T35751 T35750 punct -,old
R9310 T35752 T35745 nsubjpass mice,acclimated
R9311 T35753 T35752 amod female,mice
R9312 T35754 T35745 auxpass were,acclimated
R9313 T35755 T35745 prep for,acclimated
R9314 T35756 T35757 nummod 17,h
R9315 T35757 T35755 pobj h,for
R9316 T35758 T35745 prep before,acclimated
R9317 T35759 T35760 compound data,collection
R9318 T35760 T35758 pobj collection,before
R9319 T35761 T35745 punct .,acclimated
R9320 T35763 T35764 nsubjpass Data,collected
R9321 T35765 T35764 auxpass were,collected
R9322 T35766 T35767 det every,min
R9323 T35767 T35764 npadvmod min,collected
R9324 T35768 T35767 nummod 30,min
R9325 T35769 T35764 punct .,collected
R9326 T35771 T35772 amod Total,breaks
R9327 T35772 T35774 nsubjpass breaks,tabulated
R9328 T35773 T35772 compound beam,breaks
R9329 T35775 T35772 prep in,breaks
R9330 T35776 T35777 det the,direction
R9331 T35777 T35775 pobj direction,in
R9332 T35778 T35777 compound XY,direction
R9333 T35779 T35774 auxpass were,tabulated
R9334 T35780 T35774 prep for,tabulated
R9335 T35781 T35782 det the,cycles
R9336 T35782 T35780 pobj cycles,for
R9337 T35783 T35784 nummod 12,h
R9338 T35784 T35782 nmod h,cycles
R9339 T35785 T35784 punct -,h
R9340 T35786 T35782 nmod light,cycles
R9341 T35787 T35786 cc and,light
R9342 T35788 T35786 conj dark,light
R9343 T35789 T35774 cc and,tabulated
R9344 T35790 T35774 conj compared,tabulated
R9345 T35791 T35790 prep across,compared
R9346 T35792 T35791 pobj genotypes,across
R9347 T35793 T35774 punct .,tabulated
R9348 T36774 T36775 nmod RNA,analyses
R9349 T36776 T36774 punct ", ",RNA
R9350 T36777 T36774 conj DNA,RNA
R9351 T36778 T36777 punct ", ",DNA
R9352 T36779 T36777 cc and,DNA
R9353 T36780 T36777 conj protein,DNA
R9354 T36782 T36783 amod Total,RNA
R9355 T36783 T36784 nsubjpass RNA,isolated
R9356 T36785 T36784 auxpass was,isolated
R9357 T36786 T36784 prep by,isolated
R9358 T36787 T36788 det the,method
R9359 T36788 T36786 pobj method,by
R9360 T36789 T36788 compound RNAzol,method
R9361 T36790 T36791 punct (,Test
R9362 T36791 T36788 parataxis Test,method
R9363 T36792 T36791 compound Tel,Test
R9364 T36793 T36791 punct -,Test
R9365 T36794 T36791 punct ", ",Test
R9366 T36795 T36791 npadvmod Friendswood,Test
R9367 T36796 T36791 punct ", ",Test
R9368 T36797 T36791 npadvmod Texas,Test
R9369 T36798 T36791 punct ),Test
R9370 T36799 T36784 cc and,isolated
R9371 T36800 T36801 compound Northern,blotting
R9372 T36801 T36802 nsubjpass blotting,performed
R9373 T36802 T36784 conj performed,isolated
R9374 T36803 T36802 auxpass was,performed
R9375 T36804 T36805 mark as,described
R9376 T36805 T36802 advcl described,performed
R9377 T36806 T36805 advmod previously,described
R9378 T36807 T36808 punct [,40
R9379 T36808 T36805 parataxis 40,described
R9380 T36809 T36808 punct ],40
R9381 T36810 T36802 punct .,performed
R9382 T36812 T36813 det The,cDNAs
R9383 T36813 T36819 nsubj cDNAs,were
R9384 T36814 T36815 nmod PGC,1β
R9385 T36815 T36813 nmod 1β,cDNAs
R9386 T36816 T36815 punct -,1β
R9387 T36817 T36815 cc and,1β
R9388 T36818 T36815 conj PRC,1β
R9389 T36820 T36821 amod generous,gifts
R9390 T36821 T36819 attr gifts,were
R9391 T36822 T36821 prep from,gifts
R9392 T36823 T36824 compound Bruce,Spiegelman
R9393 T36824 T36822 pobj Spiegelman,from
R9394 T36825 T36824 cc and,Spiegelman
R9395 T36826 T36827 compound Richard,Scarpulla
R9396 T36827 T36824 conj Scarpulla,Spiegelman
R9397 T36828 T36819 punct ", ",were
R9398 T36829 T36819 advmod respectively,were
R9399 T36830 T36819 punct .,were
R9400 T36832 T36833 det The,cDNA
R9401 T36833 T36837 nsubj cDNA,was
R9402 T36834 T36833 nmod UCP,cDNA
R9403 T36835 T36834 punct -,UCP
R9404 T36836 T36834 nummod 1,UCP
R9405 T36838 T36839 det a,gift
R9406 T36839 T36837 attr gift,was
R9407 T36840 T36839 prep from,gift
R9408 T36841 T36842 compound Daniel,Ricquier
R9409 T36842 T36840 pobj Ricquier,from
R9410 T36843 T36837 punct .,was
R9411 T36845 T36846 compound RT,PCR
R9412 T36846 T36848 nsubjpass PCR,performed
R9413 T36847 T36846 punct -,PCR
R9414 T36849 T36848 auxpass was,performed
R9415 T36850 T36851 mark as,described
R9416 T36851 T36848 advcl described,performed
R9417 T36852 T36853 punct [,41
R9418 T36853 T36851 parataxis 41,described
R9419 T36854 T36853 punct ],41
R9420 T36855 T36848 punct .,performed
R9421 T36857 T36858 prep In,transcribed
R9422 T36859 T36857 amod brief,In
R9423 T36860 T36858 punct ", ",transcribed
R9424 T36861 T36862 amod total,RNA
R9425 T36862 T36858 nsubjpass RNA,transcribed
R9426 T36863 T36862 acl isolated,RNA
R9427 T36864 T36863 prep from,isolated
R9428 T36865 T36866 compound soleus,muscle
R9429 T36866 T36864 pobj muscle,from
R9430 T36867 T36866 punct ", ",muscle
R9431 T36868 T36866 conj BAT,muscle
R9432 T36869 T36868 punct ", ",BAT
R9433 T36870 T36868 cc and,BAT
R9434 T36871 T36868 conj heart,BAT
R9435 T36872 T36866 prep of,muscle
R9436 T36873 T36874 quantmod 1,2
R9437 T36874 T36876 nummod 2,mo
R9438 T36875 T36874 punct –,2
R9439 T36876 T36878 npadvmod mo,old
R9440 T36877 T36876 punct -,mo
R9441 T36878 T36880 amod old,mice
R9442 T36879 T36878 punct -,old
R9443 T36880 T36872 pobj mice,of
R9444 T36881 T36882 nmod PGC,1α
R9445 T36882 T36880 nmod 1α,mice
R9446 T36883 T36882 punct -,1α
R9447 T36884 T36882 punct +,1α
R9448 T36885 T36882 punct /,1α
R9449 T36886 T36882 punct +,1α
R9450 T36887 T36882 cc or,1α
R9451 T36888 T36882 conj PGCα,1α
R9452 T36889 T36888 punct −,PGCα
R9453 T36890 T36888 punct /,PGCα
R9454 T36891 T36888 punct −,PGCα
R9455 T36892 T36858 auxpass was,transcribed
R9456 T36893 T36858 advmod reverse,transcribed
R9457 T36894 T36858 prep with,transcribed
R9458 T36895 T36896 nmod Taqman,reagents
R9459 T36896 T36894 pobj reagents,with
R9460 T36897 T36898 amod reverse,transcription
R9461 T36898 T36896 compound transcription,reagents
R9462 T36899 T36900 punct (,Biosystems
R9463 T36900 T36896 parataxis Biosystems,reagents
R9464 T36901 T36900 compound Applied,Biosystems
R9465 T36902 T36900 punct ", ",Biosystems
R9466 T36903 T36904 compound Foster,City
R9467 T36904 T36900 npadvmod City,Biosystems
R9468 T36905 T36900 punct ", ",Biosystems
R9469 T36906 T36900 npadvmod California,Biosystems
R9470 T36907 T36900 punct ", ",Biosystems
R9471 T36908 T36909 compound United,States
R9472 T36909 T36900 npadvmod States,Biosystems
R9473 T36910 T36900 punct ),Biosystems
R9474 T36911 T36858 punct .,transcribed
R9475 T36913 T36914 nsubjpass Reactions,performed
R9476 T36915 T36914 auxpass were,performed
R9477 T36916 T36914 prep in,performed
R9478 T36917 T36916 pobj triplicate,in
R9479 T36918 T36914 prep in,performed
R9480 T36919 T36920 nummod 96,well
R9481 T36920 T36922 compound well,format
R9482 T36921 T36920 punct -,well
R9483 T36922 T36918 pobj format,in
R9484 T36923 T36914 advcl using,performed
R9485 T36924 T36925 compound Taqman,reagents
R9486 T36925 T36923 dobj reagents,using
R9487 T36926 T36925 compound core,reagents
R9488 T36927 T36925 cc and,reagents
R9489 T36928 T36929 det a,Detector
R9490 T36929 T36925 conj Detector,reagents
R9491 T36930 T36929 nmod Prism,Detector
R9492 T36931 T36930 nummod 7500,Prism
R9493 T36932 T36929 compound Sequence,Detector
R9494 T36933 T36934 punct (,Biosystems
R9495 T36934 T36929 parataxis Biosystems,Detector
R9496 T36935 T36934 compound Applied,Biosystems
R9497 T36936 T36934 punct ),Biosystems
R9498 T36937 T36914 punct .,performed
R9499 T36939 T36940 det The,sets
R9500 T36940 T36947 nsubjpass sets,found
R9501 T36941 T36942 npadvmod mouse,specific
R9502 T36942 T36940 amod specific,sets
R9503 T36943 T36942 punct -,specific
R9504 T36944 T36945 compound primer,probe
R9505 T36945 T36940 compound probe,sets
R9506 T36946 T36945 punct -,probe
R9507 T36948 T36940 acl used,sets
R9508 T36949 T36950 aux to,detect
R9509 T36950 T36948 advcl detect,used
R9510 T36951 T36952 amod specific,expression
R9511 T36952 T36950 dobj expression,detect
R9512 T36953 T36952 compound gene,expression
R9513 T36954 T36947 aux can,found
R9514 T36955 T36947 auxpass be,found
R9515 T36956 T36947 prep in,found
R9516 T36957 T36958 compound Table,S1
R9517 T36958 T36956 pobj S1,in
R9518 T36959 T36947 punct .,found
R9519 T36961 T36962 det The,primers
R9520 T36962 T36963 nsubjpass primers,described
R9521 T36964 T36962 prep for,primers
R9522 T36965 T36964 pobj UCP,for
R9523 T36966 T36965 punct -,UCP
R9524 T36967 T36965 nummod 1,UCP
R9525 T36968 T36963 aux have,described
R9526 T36969 T36963 auxpass been,described
R9527 T36970 T36963 advmod previously,described
R9528 T36971 T36972 punct [,42
R9529 T36972 T36963 parataxis 42,described
R9530 T36973 T36972 punct ],42
R9531 T36974 T36963 punct .,described
R9532 T36976 T36977 compound Actin,set
R9533 T36977 T36981 nsubjpass set,included
R9534 T36978 T36979 compound primer,probe
R9535 T36979 T36977 compound probe,set
R9536 T36980 T36979 punct -,probe
R9537 T36982 T36983 punct (,Biosystems
R9538 T36983 T36977 parataxis Biosystems,set
R9539 T36984 T36983 compound Applied,Biosystems
R9540 T36985 T36983 punct ),Biosystems
R9541 T36986 T36981 auxpass was,included
R9542 T36987 T36981 prep in,included
R9543 T36988 T36989 det a,well
R9544 T36989 T36987 pobj well,in
R9545 T36990 T36989 amod separate,well
R9546 T36991 T36981 cc and,included
R9547 T36992 T36981 conj used,included
R9548 T36993 T36994 aux to,normalize
R9549 T36994 T36992 advcl normalize,used
R9550 T36995 T36996 det the,data
R9551 T36996 T36994 dobj data,normalize
R9552 T36997 T36996 nmod soleus,data
R9553 T36998 T36997 punct ", ",soleus
R9554 T36999 T36997 conj BAT,soleus
R9555 T37000 T36999 punct ", ",BAT
R9556 T37001 T36999 cc and,BAT
R9557 T37002 T37003 compound heart,gene
R9558 T37003 T36999 conj gene,BAT
R9559 T37004 T36996 compound expression,data
R9560 T37005 T36981 punct .,included
R9561 T37007 T37008 compound GAPDH,primers
R9562 T37008 T37010 nsubjpass primers,used
R9563 T37009 T37008 compound Rodent,primers
R9564 T37011 T37012 punct (,Biosystems
R9565 T37012 T37008 parataxis Biosystems,primers
R9566 T37013 T37012 compound Applied,Biosystems
R9567 T37014 T37012 punct ),Biosystems
R9568 T37015 T37010 auxpass were,used
R9569 T37016 T37010 prep in,used
R9570 T37017 T37018 det the,well
R9571 T37018 T37016 pobj well,in
R9572 T37019 T37018 amod same,well
R9573 T37020 T37021 aux to,normalize
R9574 T37021 T37010 advcl normalize,used
R9575 T37022 T37023 det the,data
R9576 T37023 T37021 dobj data,normalize
R9577 T37024 T37025 compound liver,gene
R9578 T37025 T37023 compound gene,data
R9579 T37026 T37023 compound expression,data
R9580 T37027 T37010 punct .,used
R9581 T37029 T37030 prep For,digested
R9582 T37031 T37032 compound Southern,blot
R9583 T37032 T37033 compound blot,studies
R9584 T37033 T37029 pobj studies,For
R9585 T37034 T37030 punct ", ",digested
R9586 T37035 T37036 nummod 5,μg
R9587 T37036 T37030 nsubjpass μg,digested
R9588 T37037 T37036 prep of,μg
R9589 T37038 T37039 amod genomic,DNA
R9590 T37039 T37037 pobj DNA,of
R9591 T37040 T37030 auxpass was,digested
R9592 T37041 T37030 prep with,digested
R9593 T37042 T37041 pobj Pst1,with
R9594 T37043 T37042 cc or,Pst1
R9595 T37044 T37042 conj Xba1,Pst1
R9596 T37045 T37030 punct ", ",digested
R9597 T37046 T37030 conj electrophoresed,digested
R9598 T37047 T37046 prep on,electrophoresed
R9599 T37048 T37049 det a,gel
R9600 T37049 T37047 pobj gel,on
R9601 T37050 T37051 nummod 0.8,%
R9602 T37051 T37052 compound %,TAE
R9603 T37052 T37049 compound TAE,gel
R9604 T37053 T37046 cc and,electrophoresed
R9605 T37054 T37046 conj transferred,electrophoresed
R9606 T37055 T37054 prep to,transferred
R9607 T37056 T37057 det a,membrane
R9608 T37057 T37055 pobj membrane,to
R9609 T37058 T37059 nmod Gene,Screen
R9610 T37059 T37057 nmod Screen,membrane
R9611 T37060 T37061 punct (,Elmer
R9612 T37061 T37059 parataxis Elmer,Screen
R9613 T37062 T37061 compound Perkin,Elmer
R9614 T37063 T37061 punct ", ",Elmer
R9615 T37064 T37061 npadvmod Wellesley,Elmer
R9616 T37065 T37061 punct ", ",Elmer
R9617 T37066 T37061 npadvmod California,Elmer
R9618 T37067 T37061 punct ", ",Elmer
R9619 T37068 T37069 compound United,States
R9620 T37069 T37061 npadvmod States,Elmer
R9621 T37070 T37061 punct ),Elmer
R9622 T37071 T37054 prep for,transferred
R9623 T37072 T37071 pobj hybridization,for
R9624 T37073 T37030 punct .,digested
R9625 T37075 T37076 compound Western,blotting
R9626 T37076 T37077 nsubjpass blotting,performed
R9627 T37078 T37077 auxpass was,performed
R9628 T37079 T37080 mark as,described
R9629 T37080 T37077 advcl described,performed
R9630 T37081 T37082 punct [,43
R9631 T37082 T37080 parataxis 43,described
R9632 T37083 T37082 punct ],43
R9633 T37084 T37077 advcl using,performed
R9634 T37085 T37086 det the,system
R9635 T37086 T37084 dobj system,using
R9636 T37087 T37088 amod Enhanced,Chemiluminescence
R9637 T37088 T37086 compound Chemiluminescence,system
R9638 T37089 T37086 compound detection,system
R9639 T37090 T37091 punct (,Biotech
R9640 T37091 T37086 parataxis Biotech,system
R9641 T37092 T37091 compound Amersham,Biotech
R9642 T37093 T37091 compound Pharmacia,Biotech
R9643 T37094 T37091 punct ", ",Biotech
R9644 T37095 T37091 npadvmod Piscataway,Biotech
R9645 T37096 T37091 punct ", ",Biotech
R9646 T37097 T37098 compound New,Jersey
R9647 T37098 T37091 npadvmod Jersey,Biotech
R9648 T37099 T37091 punct ", ",Biotech
R9649 T37100 T37101 compound United,States
R9650 T37101 T37091 npadvmod States,Biotech
R9651 T37102 T37091 punct ),Biotech
R9652 T37103 T37077 punct .,performed
R9653 T37105 T37106 nmod Ponceau,S
R9654 T37106 T37107 nmod S,staining
R9655 T37107 T37119 nsubjpass staining,used
R9656 T37108 T37109 punct (,Sigma
R9657 T37109 T37106 parataxis Sigma,S
R9658 T37110 T37109 punct ", ",Sigma
R9659 T37111 T37112 compound St.,Louis
R9660 T37112 T37109 npadvmod Louis,Sigma
R9661 T37113 T37109 punct ", ",Sigma
R9662 T37114 T37109 npadvmod Missouri,Sigma
R9663 T37115 T37109 punct ", ",Sigma
R9664 T37116 T37117 compound United,States
R9665 T37117 T37109 npadvmod States,Sigma
R9666 T37118 T37109 punct ),Sigma
R9667 T37120 T37107 prep of,staining
R9668 T37121 T37122 det the,membrane
R9669 T37122 T37120 pobj membrane,of
R9670 T37123 T37119 auxpass was,used
R9671 T37124 T37119 prep as,used
R9672 T37125 T37126 det a,control
R9673 T37126 T37124 pobj control,as
R9674 T37127 T37119 punct .,used
R9675 T38198 T38199 amod Mitochondrial,respiration
R9676 T38199 T38200 compound respiration,studies
R9677 T38202 T38203 amod Mitochondrial,respiration
R9678 T38203 T38204 nsubjpass respiration,assessed
R9679 T38205 T38204 auxpass was,assessed
R9680 T38206 T38204 prep in,assessed
R9681 T38207 T38208 npadvmod saponin,skinned
R9682 T38208 T38210 amod skinned,fibers
R9683 T38209 T38208 punct -,skinned
R9684 T38210 T38206 pobj fibers,in
R9685 T38211 T38210 compound soleus,fibers
R9686 T38212 T38204 prep with,assessed
R9687 T38213 T38212 pobj succinate,with
R9688 T38214 T38213 prep as,succinate
R9689 T38215 T38214 pobj substrate,as
R9690 T38216 T38212 cc and,with
R9691 T38217 T38212 conj in,with
R9692 T38218 T38219 det the,presence
R9693 T38219 T38217 pobj presence,in
R9694 T38220 T38219 prep of,presence
R9695 T38221 T38220 pobj rotenone,of
R9696 T38222 T38223 mark as,described
R9697 T38223 T38204 advcl described,assessed
R9698 T38224 T38223 advmod previously,described
R9699 T38225 T38226 punct [,44
R9700 T38226 T38223 parataxis 44,described
R9701 T38227 T38226 punct ],44
R9702 T38228 T38204 punct .,assessed
R9703 T38230 T38231 prep In,anesthetized
R9704 T38232 T38230 amod brief,In
R9705 T38233 T38231 punct ", ",anesthetized
R9706 T38234 T38235 nummod 3,mo
R9707 T38235 T38237 npadvmod mo,old
R9708 T38236 T38235 punct -,mo
R9709 T38237 T38239 amod old,mice
R9710 T38238 T38237 punct -,old
R9711 T38239 T38231 nsubjpass mice,anesthetized
R9712 T38240 T38239 amod female,mice
R9713 T38241 T38231 auxpass were,anesthetized
R9714 T38242 T38231 prep with,anesthetized
R9715 T38243 T38244 amod chloral,hydrate
R9716 T38244 T38242 pobj hydrate,with
R9717 T38245 T38246 punct (,mg
R9718 T38246 T38244 parataxis mg,hydrate
R9719 T38247 T38246 nummod 400,mg
R9720 T38248 T38249 punct /,kg
R9721 T38249 T38246 prep kg,mg
R9722 T38250 T38249 prep of,kg
R9723 T38251 T38252 compound body,weight
R9724 T38252 T38250 pobj weight,of
R9725 T38253 T38246 punct ),mg
R9726 T38254 T38231 punct .,anesthetized
R9727 T38256 T38257 compound Soleus,fibers
R9728 T38257 T38258 nsubjpass fibers,separated
R9729 T38259 T38258 auxpass were,separated
R9730 T38260 T38258 cc and,separated
R9731 T38261 T38262 advmod then,transferred
R9732 T38262 T38258 conj transferred,separated
R9733 T38263 T38262 prep to,transferred
R9734 T38264 T38265 det a,buffer
R9735 T38265 T38263 pobj buffer,to
R9736 T38266 T38265 punct (,buffer
R9737 T38267 T38268 nummod 2.77,mM
R9738 T38268 T38269 compound mM,EGTA
R9739 T38269 T38265 appos EGTA,buffer
R9740 T38270 T38269 compound K2Ca,EGTA
R9741 T38271 T38269 punct -,EGTA
R9742 T38272 T38269 punct ", ",EGTA
R9743 T38273 T38274 nummod 7.23,mM
R9744 T38274 T38275 compound mM,K2EGTA
R9745 T38275 T38269 appos K2EGTA,EGTA
R9746 T38276 T38269 punct ", ",EGTA
R9747 T38277 T38278 nummod 6.56,mM
R9748 T38278 T38279 compound mM,MgCl2
R9749 T38279 T38269 appos MgCl2,EGTA
R9750 T38280 T38269 punct ", ",EGTA
R9751 T38281 T38282 nummod 20,mM
R9752 T38282 T38283 compound mM,imidazole
R9753 T38283 T38269 appos imidazole,EGTA
R9754 T38284 T38269 punct ", ",EGTA
R9755 T38285 T38286 nummod 53.3,mM
R9756 T38286 T38287 compound mM,MES
R9757 T38287 T38269 appos MES,EGTA
R9758 T38288 T38287 compound K,MES
R9759 T38289 T38287 punct -,MES
R9760 T38290 T38269 punct ", ",EGTA
R9761 T38291 T38292 nummod 20,mM
R9762 T38292 T38293 compound mM,taurine
R9763 T38293 T38269 appos taurine,EGTA
R9764 T38294 T38269 punct ", ",EGTA
R9765 T38295 T38296 nummod 5.3,mM
R9766 T38296 T38297 compound mM,ATP
R9767 T38297 T38269 appos ATP,EGTA
R9768 T38298 T38269 punct ", ",EGTA
R9769 T38299 T38300 nummod 15,mM
R9770 T38300 T38301 compound mM,PCr
R9771 T38301 T38269 appos PCr,EGTA
R9772 T38302 T38269 punct ", ",EGTA
R9773 T38303 T38304 nummod 3,mM
R9774 T38304 T38305 compound mM,KH2PO4
R9775 T38305 T38269 appos KH2PO4,EGTA
R9776 T38306 T38269 punct ", ",EGTA
R9777 T38307 T38308 nummod 0.5,mM
R9778 T38308 T38309 compound mM,DTT
R9779 T38309 T38269 appos DTT,EGTA
R9780 T38310 T38311 punct [,pH
R9781 T38311 T38269 parataxis pH,EGTA
R9782 T38312 T38311 nummod 7.1,pH
R9783 T38313 T38311 punct ],pH
R9784 T38314 T38265 punct ),buffer
R9785 T38315 T38265 acl supplemented,buffer
R9786 T38316 T38315 prep with,supplemented
R9787 T38317 T38318 nummod 50,μg
R9788 T38318 T38316 pobj μg,with
R9789 T38319 T38320 punct /,saponin
R9790 T38320 T38318 prep saponin,μg
R9791 T38321 T38320 compound ml,saponin
R9792 T38322 T38315 cc and,supplemented
R9793 T38323 T38315 conj permeabilized,supplemented
R9794 T38324 T38323 prep for,permeabilized
R9795 T38325 T38326 nummod 30,min
R9796 T38326 T38324 pobj min,for
R9797 T38327 T38323 prep at,permeabilized
R9798 T38328 T38329 nummod 4,°C
R9799 T38329 T38327 pobj °C,at
R9800 T38330 T38323 prep with,permeabilized
R9801 T38331 T38332 amod gentle,stirring
R9802 T38332 T38330 pobj stirring,with
R9803 T38333 T38258 punct .,separated
R9804 T38335 T38336 nsubjpass Fibers,washed
R9805 T38337 T38336 auxpass were,washed
R9806 T38338 T38336 advmod then,washed
R9807 T38339 T38336 advmod twice,washed
R9808 T38340 T38336 prep for,washed
R9809 T38341 T38342 nummod 10,min
R9810 T38342 T38340 pobj min,for
R9811 T38343 T38342 advmod each,min
R9812 T38344 T38345 punct (,EGTA
R9813 T38345 T38336 parataxis EGTA,washed
R9814 T38346 T38347 nummod 2.77,mM
R9815 T38347 T38345 compound mM,EGTA
R9816 T38348 T38345 compound K2Ca,EGTA
R9817 T38349 T38345 punct -,EGTA
R9818 T38350 T38345 punct ", ",EGTA
R9819 T38351 T38352 nummod 7.23,mM
R9820 T38352 T38353 compound mM,K2EGTA
R9821 T38353 T38345 appos K2EGTA,EGTA
R9822 T38354 T38345 punct ", ",EGTA
R9823 T38355 T38356 nummod 1.38,mM
R9824 T38356 T38357 compound mM,MgCl2
R9825 T38357 T38345 appos MgCl2,EGTA
R9826 T38358 T38345 punct ", ",EGTA
R9827 T38359 T38360 nummod 20,mM
R9828 T38360 T38361 compound mM,imidazole
R9829 T38361 T38345 appos imidazole,EGTA
R9830 T38362 T38345 punct ", ",EGTA
R9831 T38363 T38364 nummod 100,mM
R9832 T38364 T38365 compound mM,MES
R9833 T38365 T38345 appos MES,EGTA
R9834 T38366 T38365 compound K,MES
R9835 T38367 T38365 punct -,MES
R9836 T38368 T38345 punct ", ",EGTA
R9837 T38369 T38370 nummod 20,mM
R9838 T38370 T38371 compound mM,taurine
R9839 T38371 T38345 appos taurine,EGTA
R9840 T38372 T38345 punct ", ",EGTA
R9841 T38373 T38374 nummod 3,mM
R9842 T38374 T38375 compound mM,KH2PO4
R9843 T38375 T38345 appos KH2PO4,EGTA
R9844 T38376 T38345 punct ", ",EGTA
R9845 T38377 T38378 nummod 0.5,mM
R9846 T38378 T38379 compound mM,DTT
R9847 T38379 T38345 appos DTT,EGTA
R9848 T38380 T38345 punct ", ",EGTA
R9849 T38381 T38382 nummod 2,mg
R9850 T38382 T38345 appos mg,EGTA
R9851 T38383 T38384 punct /,BSA
R9852 T38384 T38382 prep BSA,mg
R9853 T38385 T38384 compound ml,BSA
R9854 T38386 T38387 punct [,pH
R9855 T38387 T38345 parataxis pH,EGTA
R9856 T38388 T38387 nummod 7.1,pH
R9857 T38389 T38387 punct ],pH
R9858 T38390 T38345 punct ),EGTA
R9859 T38391 T38336 punct .,washed
R9860 T38393 T38394 nsubjpass Respiration,measured
R9861 T38395 T38394 auxpass was,measured
R9862 T38396 T38394 prep at,measured
R9863 T38397 T38398 nummod 25,°C
R9864 T38398 T38396 pobj °C,at
R9865 T38399 T38394 advcl using,measured
R9866 T38400 T38401 det an,probe
R9867 T38401 T38399 dobj probe,using
R9868 T38402 T38401 amod optical,probe
R9869 T38403 T38404 punct (,Probe
R9870 T38404 T38401 parataxis Probe,probe
R9871 T38405 T38404 compound Oxygen,Probe
R9872 T38406 T38404 compound FOXY,Probe
R9873 T38407 T38404 punct ", ",Probe
R9874 T38408 T38409 compound Ocean,Optics
R9875 T38409 T38404 npadvmod Optics,Probe
R9876 T38410 T38404 punct ", ",Probe
R9877 T38411 T38404 npadvmod Dunedin,Probe
R9878 T38412 T38404 punct ", ",Probe
R9879 T38413 T38404 npadvmod Florida,Probe
R9880 T38414 T38404 punct ", ",Probe
R9881 T38415 T38416 compound United,States
R9882 T38416 T38404 npadvmod States,Probe
R9883 T38417 T38404 punct ),Probe
R9884 T38418 T38394 punct .,measured
R9885 T38420 T38421 prep Following,determined
R9886 T38422 T38420 pobj measurement,Following
R9887 T38423 T38422 prep of,measurement
R9888 T38424 T38425 amod basal,respiration
R9889 T38425 T38423 pobj respiration,of
R9890 T38426 T38425 nmod state,respiration
R9891 T38427 T38426 nummod 2,state
R9892 T38428 T38421 punct ", ",determined
R9893 T38429 T38430 amod maximal,respiration
R9894 T38430 T38421 nsubjpass respiration,determined
R9895 T38431 T38430 punct (,respiration
R9896 T38432 T38433 npadvmod ADP,stimulated
R9897 T38433 T38430 amod stimulated,respiration
R9898 T38434 T38433 punct -,stimulated
R9899 T38435 T38430 punct ),respiration
R9900 T38436 T38430 nmod state,respiration
R9901 T38437 T38436 nummod 3,state
R9902 T38438 T38421 auxpass was,determined
R9903 T38439 T38421 prep by,determined
R9904 T38440 T38439 pcomp exposing,by
R9905 T38441 T38440 dobj fibers,exposing
R9906 T38442 T38440 prep to,exposing
R9907 T38443 T38444 nummod 1,mM
R9908 T38444 T38445 compound mM,ADP
R9909 T38445 T38442 pobj ADP,to
R9910 T38446 T38421 punct .,determined
R9911 T38448 T38449 det The,integrity
R9912 T38449 T38450 nsubjpass integrity,assessed
R9913 T38451 T38449 prep of,integrity
R9914 T38452 T38453 det the,membrane
R9915 T38453 T38451 pobj membrane,of
R9916 T38454 T38453 amod outer,membrane
R9917 T38455 T38453 amod mitochondrial,membrane
R9918 T38456 T38450 auxpass was,assessed
R9919 T38457 T38450 prep by,assessed
R9920 T38458 T38457 pcomp adding,by
R9921 T38459 T38460 nummod 8,μM
R9922 T38460 T38461 nmod μM,c
R9923 T38461 T38458 dobj c,adding
R9924 T38462 T38461 amod exogenous,c
R9925 T38463 T38461 compound cytochrome,c
R9926 T38464 T38458 prep to,adding
R9927 T38465 T38466 npadvmod ADP,stimulated
R9928 T38466 T38468 amod stimulated,fibers
R9929 T38467 T38466 punct -,stimulated
R9930 T38468 T38464 pobj fibers,to
R9931 T38469 T38450 punct .,assessed
R9932 T38471 T38472 nmod State,respiration
R9933 T38472 T38474 nsubjpass respiration,evaluated
R9934 T38473 T38471 nummod 4,State
R9935 T38475 T38476 punct (,uncoupled
R9936 T38476 T38472 parataxis uncoupled,respiration
R9937 T38477 T38476 punct ),uncoupled
R9938 T38478 T38474 auxpass was,evaluated
R9939 T38479 T38474 prep following,evaluated
R9940 T38480 T38479 pobj addition,following
R9941 T38481 T38480 prep of,addition
R9942 T38482 T38481 pobj oligomycin,of
R9943 T38483 T38484 punct (,μg
R9944 T38484 T38482 parataxis μg,oligomycin
R9945 T38485 T38484 nummod 1,μg
R9946 T38486 T38487 punct /,ml
R9947 T38487 T38484 prep ml,μg
R9948 T38488 T38484 punct ),μg
R9949 T38489 T38474 punct .,evaluated
R9950 T38491 T38492 det The,solubility
R9951 T38492 T38493 nsubjpass solubility,taken
R9952 T38494 T38492 prep of,solubility
R9953 T38495 T38494 pobj oxygen,of
R9954 T38496 T38492 prep in,solubility
R9955 T38497 T38498 det the,buffer
R9956 T38498 T38496 pobj buffer,in
R9957 T38499 T38498 compound respiration,buffer
R9958 T38500 T38492 prep at,solubility
R9959 T38501 T38502 nummod 25,°C
R9960 T38502 T38500 pobj °C,at
R9961 T38503 T38493 auxpass was,taken
R9962 T38504 T38493 prep as,taken
R9963 T38505 T38506 nummod 246.87,nmol
R9964 T38506 T38507 compound nmol,O2
R9965 T38507 T38504 pobj O2,as
R9966 T38508 T38507 punct ·,O2
R9967 T38509 T38507 appos ml,O2
R9968 T38510 T38511 punct −,1
R9969 T38511 T38509 nummod 1,ml
R9970 T38512 T38493 punct .,taken
R9971 T38514 T38515 compound Respiration,rates
R9972 T38515 T38516 nsubjpass rates,expressed
R9973 T38517 T38516 auxpass were,expressed
R9974 T38518 T38516 prep as,expressed
R9975 T38519 T38520 compound nmol,O2
R9976 T38520 T38518 pobj O2,as
R9977 T38521 T38520 punct ·,O2
R9978 T38522 T38520 appos min,O2
R9979 T38523 T38524 punct −,1
R9980 T38524 T38522 nummod 1,min
R9981 T38525 T38520 punct ·,O2
R9982 T38526 T38520 appos mgdw,O2
R9983 T38527 T38528 punct −,1
R9984 T38528 T38526 nummod 1,mgdw
R9985 T38529 T38516 punct .,expressed
R9988 T38945 T38946 nmod Insulin,tolerance
R9989 T38946 T38949 compound tolerance,tests
R9990 T38947 T38945 cc and,Insulin
R9991 T38948 T38945 conj glucose,Insulin
R9992 T38951 T38952 nmod Glucose,tolerance
R9993 T38952 T38955 compound tolerance,tests
R9994 T38953 T38951 cc and,Glucose
R9995 T38954 T38951 conj Insulin,Glucose
R9996 T38955 T38956 nsubjpass tests,performed
R9997 T38957 T38956 auxpass were,performed
R9998 T38958 T38959 mark as,described
R9999 T38959 T38956 advcl described,performed