Id |
Subject |
Object |
Predicate |
Lexical cue |
T4013 |
28-50 |
Protein |
denotes |
total protein lysates, |
T4014 |
51-56 |
Entity |
denotes |
cells |
T4008 |
99-134 |
Entity |
denotes |
0.5% sodium deoxycholate, 0.1% SDS, |
T4012 |
125-133 |
Protein |
denotes |
0.1% SDS |
T4023 |
135-156 |
Entity |
denotes |
1 mM EDTA, 1 mM EGTA, |
T3999 |
146-155 |
Entity |
denotes |
1 mM EGTA |
T4025 |
170-179 |
Entity |
denotes |
20 mM NaF |
T3998 |
181-191 |
Entity |
denotes |
0.5 mM DTT |
T4022 |
193-202 |
Entity |
denotes |
1 mM PMSF |
T4018 |
208-216 |
Protein |
denotes |
protease |
T4002 |
208-235 |
Entity |
denotes |
protease inhibitor cocktail |
T4031 |
295-305 |
Protein |
denotes |
C. Protein |
T4005 |
354-361 |
Entity |
denotes |
Protein |
T4010 |
368-388 |
Entity |
denotes |
Reagent (Pierce, IL, |
T4032 |
377-387 |
Entity |
denotes |
Pierce, IL |
T4017 |
439-446 |
Protein |
denotes |
protein |
T4028 |
447-455 |
Entity |
denotes |
extracts |
T4016 |
520-584 |
Entity |
denotes |
by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis |
T4009 |
520-584 |
Entity |
denotes |
by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis |
T4003 |
550-564 |
Entity |
denotes |
polyacrylamide |
T4019 |
612-626 |
Entity |
denotes |
nitrocellulose |
T4026 |
612-635 |
Entity |
denotes |
nitrocellulose membrane |
T4006 |
657-666 |
Entity |
denotes |
membranes |
T4027 |
707-713 |
Entity |
denotes |
saline |
T4024 |
743-767 |
Entity |
denotes |
the following antibodies |
T4007 |
773-803 |
Protein |
denotes |
used: anti-β-actin (sc-130657, |
T4000 |
804-828 |
Entity |
denotes |
Santa Cruz Biotechnology |
T4020 |
851-888 |
Protein |
denotes |
anti-matrix metallopeptidase 9 (MMP9) |
T4021 |
883-887 |
Protein |
denotes |
MMP9 |
T4001 |
899-904 |
Entity |
denotes |
Santa |
T4015 |
948-964 |
Protein |
denotes |
primary antibody |
T4004 |
1127-1166 |
Protein |
denotes |
covered with the horseradish peroxidase |
T4011 |
1174-1192 |
Protein |
denotes |
secondary antibody |
T4030 |
1279-1282 |
Protein |
denotes |
ECL |
T4033 |
1324-1333 |
Protein |
denotes |
Bucks, UK |
T4029 |
1468-1475 |
Protein |
denotes |
β-actin |