bionlp-st-ge-2016-test-ihmc  

PMC:3317373 / 5505-7257 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3039 14-24 Entity denotes astrocytes
T3044 87-133 Entity denotes The cell pellet obtained by centrifugation was
T3093 167-178 Entity denotes 10 mM HEPES
T3080 180-182 Protein denotes pH
T3043 189-224 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3083 195-199 Entity denotes KCl,
T3091 207-212 Entity denotes EDTA,
T3094 226-237 Entity denotes 1.0 mM DTT,
T3076 242-278 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3054 285-301 Entity denotes 10% Nonidet P-40
T3060 316-324 Entity denotes vortexed
T3081 338-348 Entity denotes the nuclei
T3059 382-402 Protein denotes The nuclear proteins
T3095 382-452 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3040 441-452 Entity denotes 20 mM HEPES
T3068 454-456 Protein denotes pH
T3063 463-488 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
T3042 476-487 Entity denotes 1.0 mM EDTA
T3075 489-513 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3041 502-512 Entity denotes 1.0 mM DTT
T3070 518-554 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3072 623-670 Entity denotes the supernatant containing the nuclear proteins
T3062 650-670 Protein denotes the nuclear proteins
T3086 702-727 Entity denotes Protein concentration was
T3057 745-775 Entity denotes a bicinchoninic acid assay kit
T3092 747-765 Entity denotes bicinchoninic acid
T3082 781-801 Protein denotes bovine serum albumin
T3061 819-838 Entity denotes Pierce Biochemicals
T3090 840-858 Entity denotes Rockford, IL, USA)
T3050 885-888 Entity denotes gel
T3065 937-999 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3074 1019-1043 Entity denotes T4-polynucleotide kinase
T3056 1019-1043 Entity denotes T4-polynucleotide kinase
T3058 1022-1043 Protein denotes polynucleotide kinase
T3055 1057-1077 Entity denotes -ATP (Free Biotech.,
T3067 1095-1110 Protein denotes Nuclear protein
T3089 1171-1185 Entity denotes 10 mM Tris-HCl
T3046 1171-1185 Entity denotes 10 mM Tris-HCl
T3073 1187-1189 Protein denotes PH
T3084 1208-1233 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3071 1221-1232 Entity denotes 4% glycerol
T3078 1234-1258 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3087 1247-1257 Entity denotes 0.5 mM DTT
T3088 1268-1272 Entity denotes poly
T3053 1283-1300 Entity denotes 20 minutes on ice
T3064 1317-1361 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3045 1396-1413 Entity denotes 20 minutes on ice
T3085 1415-1440 Entity denotes The DNA-protein complexes
T3079 1478-1513 Entity denotes 4% nondenaturing polyacrylamide gel
T3049 1495-1509 Entity denotes polyacrylamide
T3069 1523-1552 Entity denotes TBE buffer (tris-borate-EDTA)
T3051 1535-1539 Entity denotes tris
T3048 1535-1551 Entity denotes tris-borate-EDTA
T3066 1535-1551 Entity denotes tris-borate-EDTA
T3047 1540-1546 Entity denotes borate
T3077 1604-1611 Entity denotes the gel
T3052 1690-1695 Protein denotes NF-κB
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES