bionlp-st-ge-2016-test-ihmc  

PMC:3317373 JSONTXT 30 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue Speculation
T567 12-19 Protein denotes of Nrf2
T574 86-119 Entity denotes Mouse Primary Cultured Astrocytes
T593 207-250 Positive_regulation denotes activation of NF-κB in the brain after TBI,
T580 218-226 Protein denotes of NF-κB
T579 302-306 Protein denotes Nrf2
T591 311-340 Protein denotes NF-κB in astrocytes after TBI
T590 320-330 Entity denotes astrocytes
T588 357-384 Entity denotes primary cultured astrocytes
T585 414-427 Protein denotes knockout mice
T594 462-485 Positive_regulation denotes the activation of NF-κB
T575 477-485 Protein denotes of NF-κB
T595 490-547 Gene_expression denotes expression of proinflammatory cytokines in a model of TBI
T586 501-529 Protein denotes of proinflammatory cytokines
T577 558-585 Entity denotes Primary cultured astrocytes
T564 671-679 Protein denotes of NF-κB
T578 707-711 Protein denotes mRNA
T582 716-723 Protein denotes protein
T581 734-739 Protein denotes TNF-α
T576 741-753 Protein denotes IL-1β, IL-6,
T566 748-752 Protein denotes IL-6
T573 758-762 Protein denotes MMP9
T584 784-802 Entity denotes Gelatin zymography
T563 840-847 Protein denotes of MMP9
T568 865-870 Protein denotes NF-κB
T592 875-924 Gene_expression denotes expression of proinflammatory cytokines mentioned
T583 886-914 Protein denotes of proinflammatory cytokines
T562 954-969 Entity denotes h after scratch
T589 999-1006 Protein denotes of MMP9
T571 1072-1076 Protein denotes Nrf2
T565 1072-1090 Protein denotes Nrf2 KO astrocytes
T570 1072-1090 Entity denotes Nrf2 KO astrocytes
T572 1104-1117 Entity denotes WT astrocytes
T569 1214-1233 Protein denotes activation of NF-κB
T587 1278-1288 Entity denotes astrocytes
T1207 1607-1632 Entity denotes of peripheral blood cells
T1197 1661-1682 Entity denotes immunocompetent cells
T1199 1744-1756 Protein denotes interleukins
T1210 1938-1942 Protein denotes Nrf2
T1215 1996-2016 Protein denotes of antioxidant genes
T1206 1999-2010 Entity denotes antioxidant
T1208 2057-2061 Protein denotes Nrf2
T1213 2205-2209 Protein denotes Nrf2
T1205 2205-2223 Protein denotes Nrf2 knockout mice
T1216 2232-2249 Protein denotes wildtype mice [5]
T1211 2335-2339 Protein denotes Nrf2
T1209 2343-2383 Entity denotes its inducers, such as sulforaphane (SFN)
T1204 2379-2382 Protein denotes SFN
T1200 2416-2426 Entity denotes astrocytes
T1202 2634-2638 Protein denotes Nrf2
T1214 2659-2669 Entity denotes astrocytes
T1218 2697-2850 Regulation denotes we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
T1198 2723-2730 Protein denotes of Nrf2
T1217 2731-2850 Gene_expression denotes on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
T1212 2749-2777 Protein denotes of proinflammatory cytokines
T1201 2781-2850 Entity denotes primary cultured astrocytes from transgenic mice after scratch injury
T1203 2814-2850 Protein denotes transgenic mice after scratch injury
T1808 2919-2938 Entity denotes of Mouse Astrocytes
T1801 2939-2943 Protein denotes Nrf2
T1809 3056-3074 Entity denotes Primary astrocytes
T1812 3056-3151 Binding denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO)
T1806 3114-3118 Protein denotes Nrf2
T1791 3148-3150 Protein denotes KO
T1810 3152-3183 Protein denotes mice (6 mice for each genotype)
T1807 3158-3182 Protein denotes 6 mice for each genotype
T1790 3368-3403 Entity denotes 0.5% trypsin for 10 minutes at 37°C
T1802 3495-3503 Protein denotes 10% FBS,
T1794 3555-3562 Protein denotes 20% FBS
T1798 3569-3588 Entity denotes units/mL penicillin
T1799 3590-3636 Entity denotes 5 μg/mL streptomycin (complete culture medium)
T1789 3659-3662 Entity denotes 1 h
T1792 3664-3679 Entity denotes the supernatant
T1803 3747-3776 Entity denotes the residual epithelial cells
T1811 3893-3903 Entity denotes Astrocytes
T1804 4004-4007 Entity denotes 4 h
T1805 4019-4032 Entity denotes the microglia
T1795 4037-4056 Entity denotes less adherent cells
T1793 4126-4137 Entity denotes astrocytes,
T1800 4184-4200 Protein denotes GFAP (sc-166481,
T1797 4201-4226 Protein denotes Santa Cruz Biotechnology,
T1796 4237-4242 Entity denotes Cells
T2028 4366-4432 Entity denotes Astrocyte scratch injury was performed as in a previous report [7]
T2027 4434-4444 Entity denotes Astrocytes
T2029 4500-4508 Entity denotes The cell
T2023 4540-4547 Protein denotes sterile
T2030 4644-4649 Entity denotes cells
T2031 4673-4723 Regulation denotes sterile PBS, cultured with complete culture medium
T2026 4673-4723 Protein denotes sterile PBS, cultured with complete culture medium
T2025 4749-4769 Entity denotes Cells, which did not
T2024 4828-4832 Entity denotes sham
T2297 4846-4850 Entity denotes Cell
T2300 4872-4877 Entity denotes CCK-8
T2299 4892-4902 Entity denotes well cells
T2304 4963-4974 Entity denotes h to adhere
T2298 4981-4989 Entity denotes the cell
T2303 5022-5031 Entity denotes Astrocyte
T2302 5059-5063 Entity denotes cell
T2306 5080-5085 Entity denotes CCK-8
T2301 5110-5128 Entity denotes 24 h after scratch
T2307 5186-5191 Entity denotes CCK-8
T2305 5236-5239 Entity denotes 1 h
T3039 5519-5529 Entity denotes astrocytes
T3044 5592-5638 Entity denotes The cell pellet obtained by centrifugation was
T3093 5672-5683 Entity denotes 10 mM HEPES
T3080 5685-5687 Protein denotes pH
T3043 5694-5729 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3083 5700-5704 Entity denotes KCl,
T3091 5712-5717 Entity denotes EDTA,
T3094 5731-5742 Entity denotes 1.0 mM DTT,
T3076 5747-5783 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3054 5790-5806 Entity denotes 10% Nonidet P-40
T3060 5821-5829 Entity denotes vortexed
T3081 5843-5853 Entity denotes the nuclei
T3059 5887-5907 Protein denotes The nuclear proteins
T3095 5887-5957 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3040 5946-5957 Entity denotes 20 mM HEPES
T3068 5959-5961 Protein denotes pH
T3063 5968-5993 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
T3042 5981-5992 Entity denotes 1.0 mM EDTA
T3075 5994-6018 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3041 6007-6017 Entity denotes 1.0 mM DTT
T3070 6023-6059 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3072 6128-6175 Entity denotes the supernatant containing the nuclear proteins
T3062 6155-6175 Protein denotes the nuclear proteins
T3086 6207-6232 Entity denotes Protein concentration was
T3057 6250-6280 Entity denotes a bicinchoninic acid assay kit
T3092 6252-6270 Entity denotes bicinchoninic acid
T3082 6286-6306 Protein denotes bovine serum albumin
T3061 6324-6343 Entity denotes Pierce Biochemicals
T3090 6345-6363 Entity denotes Rockford, IL, USA)
T3050 6390-6393 Entity denotes gel
T3065 6442-6504 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3074 6524-6548 Entity denotes T4-polynucleotide kinase
T3056 6524-6548 Entity denotes T4-polynucleotide kinase
T3058 6527-6548 Protein denotes polynucleotide kinase
T3055 6562-6582 Entity denotes -ATP (Free Biotech.,
T3067 6600-6615 Protein denotes Nuclear protein
T3089 6676-6690 Entity denotes 10 mM Tris-HCl
T3046 6676-6690 Entity denotes 10 mM Tris-HCl
T3073 6692-6694 Protein denotes PH
T3084 6713-6738 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3071 6726-6737 Entity denotes 4% glycerol
T3078 6739-6763 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3087 6752-6762 Entity denotes 0.5 mM DTT
T3088 6773-6777 Entity denotes poly
T3053 6788-6805 Entity denotes 20 minutes on ice
T3064 6822-6866 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3045 6901-6918 Entity denotes 20 minutes on ice
T3085 6920-6945 Entity denotes The DNA-protein complexes
T3079 6983-7018 Entity denotes 4% nondenaturing polyacrylamide gel
T3049 7000-7014 Entity denotes polyacrylamide
T3069 7028-7057 Entity denotes TBE buffer (tris-borate-EDTA)
T3051 7040-7044 Entity denotes tris
T3048 7040-7056 Entity denotes tris-borate-EDTA
T3066 7040-7056 Entity denotes tris-borate-EDTA
T3047 7045-7051 Entity denotes borate
T3077 7109-7116 Entity denotes the gel
T3052 7195-7200 Protein denotes NF-κB
T3358 7264-7270 Protein denotes RT-PCR
T3366 7271-7280 Protein denotes Total RNA
T3361 7299-7318 Entity denotes Trizol (Invitrogen,
T3364 7333-7353 Entity denotes single-stranded cDNA
T3359 7375-7392 Protein denotes 2 μg of total RNA
T3362 7380-7392 Protein denotes of total RNA
T3360 7398-7441 Protein denotes BU-Script RT-Kit (Biunique, Jiangsu, China)
T3363 7484-7492 Entity denotes The cDNA
T3355 7670-7673 Protein denotes PCR
T3365 7686-7689 Protein denotes PCR
T3356 7716-7723 Entity denotes agarose
T3357 7716-7743 Entity denotes agarose gel electrophoresis
T3354 7817-7824 Protein denotes β-actin
T4013 7888-7910 Protein denotes total protein lysates,
T4014 7911-7916 Entity denotes cells
T4008 7959-7994 Entity denotes 0.5% sodium deoxycholate, 0.1% SDS,
T4012 7985-7993 Protein denotes 0.1% SDS
T4023 7995-8016 Entity denotes 1 mM EDTA, 1 mM EGTA,
T3999 8006-8015 Entity denotes 1 mM EGTA
T4025 8030-8039 Entity denotes 20 mM NaF
T3998 8041-8051 Entity denotes 0.5 mM DTT
T4022 8053-8062 Entity denotes 1 mM PMSF
T4018 8068-8076 Protein denotes protease
T4002 8068-8095 Entity denotes protease inhibitor cocktail
T4031 8155-8165 Protein denotes C. Protein
T4005 8214-8221 Entity denotes Protein
T4010 8228-8248 Entity denotes Reagent (Pierce, IL,
T4032 8237-8247 Entity denotes Pierce, IL
T4017 8299-8306 Protein denotes protein
T4028 8307-8315 Entity denotes extracts
T4016 8380-8444 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4009 8380-8444 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4003 8410-8424 Entity denotes polyacrylamide
T4019 8472-8486 Entity denotes nitrocellulose
T4026 8472-8495 Entity denotes nitrocellulose membrane
T4006 8517-8526 Entity denotes membranes
T4027 8567-8573 Entity denotes saline
T4024 8603-8627 Entity denotes the following antibodies
T4007 8633-8663 Protein denotes used: anti-β-actin (sc-130657,
T4000 8664-8688 Entity denotes Santa Cruz Biotechnology
T4020 8711-8748 Protein denotes anti-matrix metallopeptidase 9 (MMP9)
T4021 8743-8747 Protein denotes MMP9
T4001 8759-8764 Entity denotes Santa
T4015 8808-8824 Protein denotes primary antibody
T4004 8987-9026 Protein denotes covered with the horseradish peroxidase
T4011 9034-9052 Protein denotes secondary antibody
T4030 9139-9142 Protein denotes ECL
T4033 9184-9193 Protein denotes Bucks, UK
T4029 9328-9335 Protein denotes β-actin
T4345 9415-9471 Entity denotes Cells of four groups were homogenized as mentioned above
T4353 9473-9488 Entity denotes The supernatant
T4349 9508-9521 Protein denotes total protein
T4357 9555-9576 Entity denotes Protein Assay Reagent
T4344 9555-9576 Entity denotes Protein Assay Reagent
T4341 9555-9576 Entity denotes Protein Assay Reagent
T4347 9586-9588 Entity denotes IL
T4346 9603-9641 Protein denotes of tumor necrosis factor-alpha (TNF-α)
T4355 9635-9640 Protein denotes TNF-α
T4338 9643-9670 Protein denotes interleukin-1 beta (IL-1β),
T4359 9663-9668 Protein denotes IL-1β
T4350 9675-9688 Protein denotes interleukin-6
T4356 9690-9694 Protein denotes IL-6
T4361 9696-9703 Protein denotes protein
T4340 9802-9824 Entity denotes Bender MedSystems Inc.
T4360 10014-10038 Entity denotes 2 h at room temperature,
T4358 10102-10118 Protein denotes streptavidin-HRP
T4354 10102-10118 Protein denotes streptavidin-HRP
T4352 10115-10118 Protein denotes HRP
T4348 10164-10168 Entity denotes 1 h,
T4343 10225-10238 Entity denotes TMB substrate
T4342 10277-10294 Entity denotes the stop solution
T4351 10441-10451 Protein denotes of protein
T4339 10525-10538 Protein denotes total protein
T4752 10546-10554 Entity denotes Gelatine
T4749 10566-10586 Entity denotes Cells of four groups
T4742 10631-10645 Entity denotes 50 mM Tris-HCl
T4734 10631-10645 Entity denotes 50 mM Tris-HCl
T4740 10656-10667 Entity denotes 150 mM NaCl
T4744 10681-10710 Entity denotes 0.2 mM NaN3, and 0.01% Triton
T4743 10698-10710 Entity denotes 0.01% Triton
T4746 10712-10728 Entity denotes Soluble extracts
T4736 10783-10790 Entity denotes Gelatin
T4750 10905-10937 Entity denotes 40 μg cytosolic protein extracts
T4737 10905-10937 Protein denotes 40 μg cytosolic protein extracts
T4735 10978-10990 Protein denotes the proteins
T4747 11023-11040 Entity denotes 2.5% Triton X-100
T4751 11063-11072 Entity denotes substrate
T4745 11107-11115 Protein denotes the MMP9
T4739 11163-11171 Entity denotes each gel
T4748 11208-11211 Entity denotes 1 h
T4733 11229-11241 Entity denotes 50% methanol
T4741 11296-11347 Entity denotes blue gel which indicates the lysis of the substrate
T4738 11367-11371 Protein denotes MMP9
T4841 11485-11494 Entity denotes mean ± SD
T4840 11512-11517 Protein denotes ANOVA
T4842 11522-11525 Protein denotes LSD
T5091 11687-11694 Protein denotes of Nrf2
T5101 11706-11755 Entity denotes the Cell Death in Astrocytes after Scratch Injury
T5093 11710-11714 Entity denotes Cell
T5100 11724-11734 Entity denotes Astrocytes
T5094 11798-11803 Protein denotes CCK-8
T5098 11819-11834 Entity denotes h after scratch
T5096 11871-11880 Entity denotes cell lost
T5097 11977-11979 Protein denotes KO
T5090 11985-11990 Protein denotes CCK-8
T5095 12033-12035 Protein denotes KO
T5099 12148-12171 Protein denotes group KO TBI (Figure 1)
T5092 12209-12222 Entity denotes sham-operated
T5355 12237-12255 Negative_regulation denotes Disruption of Nrf2
T5356 12237-12328 Positive_regulation denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T5347 12248-12255 Protein denotes of Nrf2
T5343 12278-12307 Protein denotes of NF-κB DNA-Binding Activity
T5344 12287-12298 Entity denotes DNA-Binding
T5353 12350-12360 Protein denotes that NF-κB
T5354 12350-12396 Positive_regulation denotes that NF-κB is activated in brain after TBI [2]
T5351 12440-12448 Protein denotes of NF-κB
T5342 12452-12486 Entity denotes astrocytes from Nrf2 WT or KO mice
T5349 12468-12475 Protein denotes Nrf2 WT
T5340 12479-12481 Protein denotes KO
T5352 12479-12486 Protein denotes KO mice
T5346 12498-12516 Entity denotes 24 h after scratch
T5345 12559-12567 Protein denotes of NF-κB
T5348 12571-12600 Entity denotes astrocytes of both genotypes,
T5341 12614-12619 Protein denotes NF-κB
T5350 12651-12653 Protein denotes KO
T6385 12798-12802 Protein denotes Nrf2
T6406 12803-12822 Entity denotes Knockout Astrocytes
T6389 12860-12865 Protein denotes TNF-α
T6412 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6411 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6410 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6397 12867-12872 Protein denotes IL-1β
T6395 12878-12882 Protein denotes IL-6
T6398 12897-12905 Protein denotes by NF-κB
T6401 12942-12950 Protein denotes of NF-κB
T6376 13167-13189 Protein denotes of Nrf2-deficient mice
T6372 13262-13277 Protein denotes these cytokines
T6413 13282-13310 Localization denotes Nrf2 in astrocytes after TBI
T6407 13282-13310 Protein denotes Nrf2 in astrocytes after TBI
T6382 13290-13310 Entity denotes astrocytes after TBI
T6373 13342-13347 Protein denotes TNF-α
T6399 13349-13354 Protein denotes IL-1β
T6414 13360-13394 Localization denotes IL-6 in cultured astrocytes at 24 
T6377 13360-13394 Protein denotes IL-6 in cultured astrocytes at 24 
T6409 13368-13387 Entity denotes cultured astrocytes
T6384 13394-13426 Entity denotes h after scratch injury by RT-PCR
T6403 13417-13426 Protein denotes by RT-PCR
T6375 13460-13464 Protein denotes mRNA
T6368 13475-13480 Protein denotes TNF-α
T6394 13482-13487 Protein denotes IL-1β
T6386 13493-13497 Protein denotes IL-6
T6405 13542-13555 Entity denotes KO astrocytes
T6400 13542-13555 Protein denotes KO astrocytes
T6381 13575-13585 Entity denotes their sham
T6396 13628-13632 Protein denotes mRNA
T6388 13643-13674 Protein denotes these cytokines in group KO TBI
T6387 13668-13670 Protein denotes KO
T6391 13757-13763 Protein denotes TNF-α,
T6378 13801-13806 Protein denotes IL-1β
T6371 13849-13853 Protein denotes IL-6
T6370 13921-13947 Protein denotes the protein level of TNF-α
T6390 13942-13947 Protein denotes TNF-α
T6404 13995-13997 Protein denotes KO
T6393 13995-14008 Entity denotes KO astrocytes
T6408 14034-14038 Entity denotes sham
T6402 14085-14087 Protein denotes KO
T6379 14223-14227 Protein denotes IL-6
T6369 14232-14237 Protein denotes IL-1β
T6383 14243-14247 Protein denotes IL-6
T6392 14280-14282 Protein denotes KO
T6380 14354-14358 Protein denotes IL-1
T6374 14393-14395 Protein denotes KO
T6994 14494-14501 Protein denotes of MMP9
T6995 14527-14531 Protein denotes Nrf2
T7000 14532-14551 Entity denotes Knockout Astrocytes
T6992 14573-14577 Protein denotes MMP9
T6988 14688-14697 Protein denotes the mRNA,
T7003 14698-14705 Protein denotes protein
T6991 14727-14734 Protein denotes of MMP9
T6996 14738-14748 Entity denotes astrocytes
T6989 14755-14826 Entity denotes h after scratch injury by RT-PCR, western blot, and gelatine zymography
T6987 14781-14787 Protein denotes RT-PCR
T6993 14807-14815 Entity denotes gelatine
T7009 14828-14846 Gene_expression denotes Expression of MMP9
T6999 14839-14846 Protein denotes of MMP9
T7001 14891-14893 Protein denotes KO
T7005 14891-14904 Entity denotes KO astrocytes
T6997 14928-14932 Entity denotes sham
T7006 14951-14955 Protein denotes mRNA
T7008 14965-14969 Protein denotes MMP9
T7004 14990-14992 Protein denotes KO
T6986 15134-15138 Protein denotes MMP9
T7002 15196-15198 Protein denotes KO
T7007 15260-15268 Entity denotes Gelatine
T6998 15296-15300 Protein denotes MMP9
T6990 15319-15321 Protein denotes KO
T10091 15490-15536 Positive_regulation denotes the upregulation of NF-κB DNA binding activity
T9967 15510-15515 Protein denotes NF-κB
T10073 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10072 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10071 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10070 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10069 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10068 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10067 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10066 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T9974 15559-15564 Protein denotes TNF-α
T10008 15566-15571 Protein denotes IL-1β
T10009 15573-15577 Protein denotes IL-6
T9965 15583-15587 Protein denotes MMP9
T9990 15591-15610 Entity denotes cultured astrocytes
T10096 15634-15734 Positive_regulation denotes scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines
T9942 15673-15681 Protein denotes of NF-κB
T10074 15695-15734 Gene_expression denotes expression of proinflammatory cytokines
T10014 15706-15734 Protein denotes of proinflammatory cytokines
T10050 15761-15771 Entity denotes astrocytes
T10036 15786-15824 Entity denotes wildtype astrocytes for the first time
T9947 15851-15861 Protein denotes that NF-κB
T10075 15851-15922 Positive_regulation denotes that NF-κB is activated in brain and spinal cord after traumatic injury
T9952 15934-15946 Protein denotes a transcript
T10034 15955-15960 Protein denotes NF-κB
T10054 15955-15997 Binding denotes NF-κB binds with DNA once it was activated
T10002 15967-15975 Entity denotes with DNA
T10064 16010-16031 Transcription denotes transcription of MMP9
T9960 16024-16031 Protein denotes of MMP9
T9940 16065-16108 Protein denotes cytokines, including TNF-α, IL-1β, and IL-6
T10027 16086-16091 Protein denotes TNF-α
T9948 16093-16098 Protein denotes IL-1β
T9984 16104-16108 Protein denotes IL-6
T9996 16217-16268 Protein denotes NF-κB DNA binding activity, overexpression of MMP9,
T10037 16260-16268 Protein denotes of MMP9,
T10035 16318-16337 Entity denotes cultured astrocytes
T10042 16453-16458 Protein denotes TNF-α
T9979 16463-16468 Protein denotes IL-1β
T9941 16496-16500 Protein denotes MMP9
T9953 16504-16519 Entity denotes astrocytes [12,
T9962 16529-16533 Protein denotes IL-6
T10098 16542-16564 Positive_regulation denotes overexpression of MMP9
T10097 16542-16564 Gene_expression denotes overexpression of MMP9
T9957 16557-16564 Protein denotes of MMP9
T9983 16606-16611 Protein denotes TNF-α
T9981 16616-16621 Protein denotes IL-1β
T10055 16638-16666 Positive_regulation denotes activation of NF-κB [15, 16]
T10015 16649-16657 Protein denotes of NF-κB
T10058 17004-17037 Binding denotes accumulation of toxic metabolites
T10020 17017-17037 Entity denotes of toxic metabolites
T10030 17117-17140 Protein denotes of Nrf2 in brain tissue
T10065 17117-17141 Localization denotes of Nrf2 in brain tissue
T9995 17218-17229 Entity denotes antioxidant
T9968 17218-17236 Protein denotes antioxidant genes,
T10040 17245-17268 Protein denotes heme oxygenase-1 (HO-1)
T10016 17245-17315 Entity denotes heme oxygenase-1 (HO-1) and NAD (P)H: quinone oxidoreductase-1 (NQO1),
T10021 17263-17267 Protein denotes HO-1
T10049 17273-17280 Entity denotes NAD (P)
T9955 17278-17279 Entity denotes P
T9988 17283-17305 Protein denotes quinone oxidoreductase
T10052 17309-17313 Protein denotes NQO1
T10005 17403-17407 Protein denotes SFN,
T10044 17550-17555 Entity denotes lipid
T9992 17592-17595 Protein denotes CA3
T10000 17655-17674 Protein denotes Nrf2-deficient mice
T10039 17717-17724 Protein denotes of Nrf2
T10084 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10083 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10082 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10081 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10080 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10079 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10078 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10077 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T9946 17855-17860 Protein denotes TNF-α
T10031 17862-17867 Protein denotes IL-1β
T10007 17869-17873 Protein denotes IL-6
T10024 17879-17897 Protein denotes MMP9 after scratch
T9980 17942-17961 Entity denotes knockout astrocytes
T9971 18069-18094 Entity denotes to more astrocytes deaths
T10041 18159-18170 Entity denotes antioxidant
T10006 18178-18185 Protein denotes of Nrf2
T10085 18264-18282 Positive_regulation denotes by NF-κB signaling
T9956 18267-18272 Protein denotes NF-κB
T10003 18302-18305 Protein denotes SFN
T9985 18365-18368 Protein denotes p65
T9945 18365-18404 Protein denotes p65 NF-κB subunit nuclear translocation
T9958 18431-18436 Protein denotes NF-κB
T10076 18431-18446 Positive_regulation denotes NF-κB signaling
T10038 18457-18532 Protein denotes NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by
T10057 18457-18532 Negative_regulation denotes NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by true
T10001 18482-18509 Entity denotes by lipopolysaccharide (LPS)
T10012 18505-18508 Entity denotes LPS
T9998 18546-18556 Entity denotes activators
T9977 18566-18569 Protein denotes SFN
T10051 18574-18588 Entity denotes curcumin (CUR)
T10013 18685-18690 Protein denotes NF-κB
T9966 18849-18868 Entity denotes cultured astrocytes
T10056 18891-18919 Positive_regulation denotes Enhanced activation of NF-κB
T10059 18891-18992 Positive_regulation denotes Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts
T9972 18911-18919 Protein denotes of NF-κB
T9954 18948-18959 Entity denotes macrophages
T10048 18965-18992 Entity denotes mouse embryonic fibroblasts
T10023 19011-19046 Protein denotes mice after experimental sepsis [24]
T10086 19078-19130 Negative_regulation denotes that NF-κB can inhibit Nrf2 at transcriptional level true
T9999 19083-19088 Protein denotes NF-κB
T10011 19101-19105 Protein denotes Nrf2
T9944 19132-19137 Protein denotes NF-κB
T9987 19132-19149 Protein denotes NF-κB p65 subunit
T9949 19233-19256 Entity denotes with the CH1-KIX domain
T9978 19246-19249 Entity denotes KIX
T9975 19260-19263 Protein denotes CBP
T9991 19273-19280 Entity denotes histone
T9963 19374-19379 Protein denotes NF-κB
T9982 19384-19389 Protein denotes Nrf2,
T9969 19413-19482 Protein denotes by the upstream mitogen-activated protein kinase (MAPKs) pathway [26]
T9994 19463-19468 Protein denotes MAPKs
T10043 19511-19515 Protein denotes Nrf2
T10019 19538-19559 Entity denotes nucleus of astrocytes
T10010 19549-19559 Entity denotes astrocytes
T10029 19596-19605 Protein denotes that Nrf2
T10004 19609-19676 Protein denotes a transcription factor which should take function mainly in nucleus
T10045 19669-19676 Entity denotes nucleus
T9939 19702-19712 Entity denotes astrocytes
T9943 19743-19792 Entity denotes cells in activation of Nrf2-ARE pathway after TBI
T10101 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10100 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10099 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10018 19838-19843 Protein denotes TNF-α
T9986 19845-19850 Protein denotes IL-1β
T9997 19856-19860 Protein denotes IL-6
T9973 19864-19883 Entity denotes cultured astrocytes
T10047 19905-19908 Entity denotes LPS
T9959 19912-19925 Entity denotes oxyhemoglobin
T9993 20004-20008 Protein denotes MMP9
T9938 20009-20028 Entity denotes positive astrocytes
T10033 20122-20135 Entity denotes of astrocytes
T9951 20246-20263 Entity denotes astrocytes, Nrf2,
T9976 20258-20262 Protein denotes Nrf2
T10063 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10062 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10061 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10060 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10095 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10094 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10093 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10092 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T9964 20360-20365 Protein denotes TNF-α
T9970 20367-20372 Protein denotes IL-1β
T10026 20374-20378 Protein denotes IL-6
T10028 20384-20388 Protein denotes MMP9
T10032 20406-20416 Entity denotes astrocytes
T10053 20499-20522 Protein denotes the activation of NF-κB
T10090 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10089 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10088 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10087 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T9989 20545-20550 Protein denotes TNF-α
T10017 20552-20557 Protein denotes IL-1β
T9950 20559-20563 Protein denotes IL-6
T10022 20569-20573 Protein denotes MMP9
T9961 20607-20617 Entity denotes astrocytes
T10025 20667-20671 Protein denotes Nrf2
T10046 20703-20738 Entity denotes anti-inflammatory therapy after TBI
R1725 T2026 T2031 themeOf "sterile PBS, cultured with complete culture medium","sterile PBS, cultured with complete culture medium"
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES
R439 T586 T595 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines in a model of TBI
R448 T575 T594 themeOf of NF-κB,the activation of NF-κB
R449 T580 T593 themeOf of NF-κB,"activation of NF-κB in the brain after TBI,"
R450 T583 T592 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines mentioned
R4551 T5347 T5355 themeOf of Nrf2,Disruption of Nrf2
R4552 T5353 T5354 themeOf that NF-κB,that NF-κB is activated in brain after TBI [2]
R4553 T5355 T5356 causeOf Disruption of Nrf2,Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
R5361 T6377 T6414 themeOf IL-6 in cultured astrocytes at 24 ,IL-6 in cultured astrocytes at 24 
R5362 T6382 T6413 locationOf astrocytes after TBI,Nrf2 in astrocytes after TBI
R5363 T6389 T6411 themeOf TNF-α,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5364 T6395 T6410 themeOf IL-6,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5365 T6397 T6412 themeOf IL-1β,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5366 T6398 T6410 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5367 T6398 T6411 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5368 T6398 T6412 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5369 T6407 T6413 themeOf Nrf2 in astrocytes after TBI,Nrf2 in astrocytes after TBI
R5370 T6409 T6414 locationOf cultured astrocytes,IL-6 in cultured astrocytes at 24 
R5808 T6999 T7009 themeOf of MMP9,Expression of MMP9
R8178 T9946 T10077 themeOf TNF-α,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8179 T9946 T10084 themeOf TNF-α,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8180 T9947 T10075 themeOf that NF-κB,that NF-κB is activated in brain and spinal cord after traumatic injury
R8182 T9950 T10087 themeOf IL-6,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8184 T9956 T10085 causeOf NF-κB,by NF-κB signaling
R8187 T9957 T10097 themeOf of MMP9,overexpression of MMP9
R8188 T9957 T10098 themeOf of MMP9,overexpression of MMP9
R8189 T9958 T10076 causeOf NF-κB,NF-κB signaling
R8192 T9960 T10064 themeOf of MMP9,transcription of MMP9
R8195 T9964 T10062 themeOf TNF-α,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8196 T9965 T10066 themeOf MMP9,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8197 T9965 T10071 themeOf MMP9,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8198 T9970 T10061 themeOf IL-1β,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8200 T9972 T10059 themeOf of NF-κB,"Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts"
R8203 T9974 T10070 themeOf TNF-α,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8204 T9974 T10073 themeOf TNF-α,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8207 T10014 T10074 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines
R8208 T10015 T10055 themeOf of NF-κB,"activation of NF-κB [15, 16]"
R8209 T9986 T10101 themeOf IL-1β,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8210 T10017 T10090 themeOf IL-1β,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8211 T9989 T10088 themeOf TNF-α,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8212 T10018 T10099 themeOf TNF-α,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8213 T10022 T10089 themeOf MMP9,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8214 T9997 T10100 themeOf IL-6,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8215 T10024 T10079 themeOf MMP9 after scratch,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8216 T9999 T10086 causeOf NF-κB,that NF-κB can inhibit Nrf2 at transcriptional level
R8217 T10024 T10081 themeOf MMP9 after scratch,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8218 T10002 T10054 themeOf with DNA,NF-κB binds with DNA once it was activated
R8219 T10026 T10060 themeOf IL-6,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8220 T10028 T10063 themeOf MMP9,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8221 T10007 T10078 themeOf IL-6,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8222 T10007 T10082 themeOf IL-6,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8223 T10008 T10067 themeOf IL-1β,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8224 T10008 T10069 themeOf IL-1β,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8225 T10009 T10068 themeOf IL-6,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8226 T10009 T10072 themeOf IL-6,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8227 T10011 T10086 themeOf Nrf2,that NF-κB can inhibit Nrf2 at transcriptional level
R8228 T10030 T10065 themeOf of Nrf2 in brain tissue,of Nrf2 in brain tissue
R8229 T10031 T10080 themeOf IL-1β,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8230 T10031 T10083 themeOf IL-1β,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8231 T10034 T10054 themeOf NF-κB,NF-κB binds with DNA once it was activated
R8232 T10038 T10057 causeOf NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by,NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by
R8233 T10059 T10056 themeOf "Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts",Enhanced activation of NF-κB
R8234 T10060 T10094 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8235 T10061 T10095 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8236 T10062 T10092 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8237 T10063 T10093 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8238 T10074 T10096 themeOf expression of proinflammatory cytokines,scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines
R982 T1198 T1218 causeOf of Nrf2,we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
R983 T1212 T1217 themeOf of proinflammatory cytokines,on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
R984 T1217 T1218 themeOf on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury,we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury