Id |
Subject |
Object |
Predicate |
Lexical cue |
T15439 |
13-63 |
Entity |
denotes |
downstream effector molecules of the PI3K pathway, |
T15435 |
24-32 |
Entity |
denotes |
effector |
T15433 |
46-62 |
Protein |
denotes |
the PI3K pathway |
T15446 |
64-118 |
Positive_regulation |
denotes |
we evaluated the activation of Akt by western blotting |
T15429 |
92-98 |
Protein |
denotes |
of Akt |
T15444 |
169-188 |
Protein |
denotes |
anti-CD3 (10 μg/ml) |
T15428 |
192-200 |
Entity |
denotes |
LY294002 |
T15432 |
227-260 |
Protein |
denotes |
the amounts of phosphorylated Akt |
T15427 |
327-350 |
Protein |
denotes |
Akt increased (lane 2), |
T15425 |
380-400 |
Entity |
denotes |
by LY294002 (lane 3) |
T15434 |
464-486 |
Protein |
denotes |
non-phosphorylated Akt |
T15449 |
464-486 |
Phosphorylation |
denotes |
non-phosphorylated Akt |
T15442 |
491-498 |
Protein |
denotes |
β-actin |
T15430 |
549-552 |
Protein |
denotes |
PHA |
T15448 |
549-658 |
Regulation |
denotes |
PHA, concanavalin A and IL-15 also demonstrated the same effect on phosphorylated Akt as shown with anti-CD3, |
T15437 |
554-568 |
Protein |
denotes |
concanavalin A |
T15438 |
573-578 |
Protein |
denotes |
IL-15 |
T15447 |
613-634 |
Phosphorylation |
denotes |
on phosphorylated Akt |
T15441 |
613-634 |
Protein |
denotes |
on phosphorylated Akt |
T15436 |
649-658 |
Protein |
denotes |
anti-CD3, |
T15443 |
686-696 |
Entity |
denotes |
wortmannin |
T15445 |
720-745 |
Entity |
denotes |
LY294002 (data not shown) |
R8527 |
T15429 |
T15446 |
themeOf |
of Akt,we evaluated the activation of Akt by western blotting |
R8528 |
T15434 |
T15449 |
themeOf |
non-phosphorylated Akt,non-phosphorylated Akt |
R8529 |
T15441 |
T15447 |
themeOf |
on phosphorylated Akt,on phosphorylated Akt |
R8530 |
T15441 |
T15448 |
themeOf |
on phosphorylated Akt,"PHA, concanavalin A and IL-15 also demonstrated the same effect on phosphorylated Akt as shown with anti-CD3," |