Id |
Subject |
Object |
Predicate |
Lexical cue |
T9881 |
0-4 |
NNP |
denotes |
Cell |
T9882 |
5-12 |
NNP |
denotes |
Culture |
T9883 |
13-15 |
NNP |
denotes |
CF |
T9884 |
16-17 |
-LRB- |
denotes |
( |
T9885 |
17-22 |
NNP |
denotes |
IB3-1 |
T9886 |
23-26 |
CC |
denotes |
and |
T9887 |
27-33 |
NNP |
denotes |
CuFi-1 |
T9888 |
33-34 |
-RRB- |
denotes |
) |
T9889 |
35-38 |
CC |
denotes |
and |
T9890 |
39-46 |
NN |
denotes |
control |
T9891 |
47-48 |
-LRB- |
denotes |
( |
T9892 |
48-50 |
CD |
denotes |
S9 |
T9893 |
51-54 |
CC |
denotes |
and |
T9894 |
55-61 |
NNP |
denotes |
NuLi-1 |
T9895 |
61-62 |
-RRB- |
denotes |
) |
T9896 |
63-68 |
NNS |
denotes |
cells |
T9897 |
69-73 |
VBD |
denotes |
were |
T9898 |
74-82 |
VBN |
denotes |
obtained |
T9899 |
83-87 |
IN |
denotes |
from |
T9900 |
88-91 |
DT |
denotes |
the |
T9901 |
92-100 |
JJ |
denotes |
American |
T9902 |
101-105 |
NNP |
denotes |
Type |
T9903 |
106-113 |
NNP |
denotes |
Culture |
T9904 |
114-124 |
NNP |
denotes |
Collection |
T9905 |
124-125 |
. |
denotes |
. |
T9906 |
126-131 |
CD |
denotes |
IB3-1 |
T9907 |
132-137 |
NNS |
denotes |
cells |
T9908 |
138-142 |
VBD |
denotes |
were |
T9909 |
143-150 |
VBN |
denotes |
derived |
T9910 |
151-155 |
IN |
denotes |
from |
T9911 |
156-157 |
DT |
denotes |
a |
T9912 |
158-165 |
JJ |
denotes |
patient |
T9913 |
166-176 |
VBG |
denotes |
expressing |
T9914 |
177-180 |
DT |
denotes |
the |
T9915 |
181-186 |
NNP |
denotes |
ΔF508 |
T9916 |
187-190 |
CC |
denotes |
and |
T9917 |
191-197 |
NNP |
denotes |
W1282X |
T9918 |
198-207 |
NNS |
denotes |
mutations |
T9919 |
208-211 |
CC |
denotes |
and |
T9920 |
212-218 |
NN |
denotes |
CuFi-1 |
T9921 |
219-223 |
VBD |
denotes |
were |
T9922 |
224-231 |
VBN |
denotes |
derived |
T9923 |
232-236 |
IN |
denotes |
from |
T9924 |
237-238 |
DT |
denotes |
a |
T9925 |
239-244 |
NNP |
denotes |
ΔF508 |
T9926 |
245-255 |
JJ |
denotes |
homozygous |
T9927 |
256-263 |
NN |
denotes |
patient |
T9928 |
263-264 |
. |
denotes |
. |
T9929 |
265-267 |
CD |
denotes |
S9 |
T9930 |
268-273 |
NNS |
denotes |
cells |
T9931 |
274-277 |
VBP |
denotes |
are |
T9932 |
278-283 |
JJ |
denotes |
IB3-1 |
T9933 |
284-289 |
NNS |
denotes |
cells |
T9934 |
290-294 |
WDT |
denotes |
that |
T9935 |
295-299 |
VBP |
denotes |
have |
T9936 |
300-304 |
VBN |
denotes |
been |
T9937 |
305-316 |
VBN |
denotes |
transfected |
T9938 |
317-321 |
IN |
denotes |
with |
T9939 |
322-326 |
NNP |
denotes |
CFTR |
T9940 |
327-332 |
VBG |
denotes |
using |
T9941 |
333-335 |
DT |
denotes |
an |
T9942 |
336-352 |
JJ |
denotes |
adeno-associated |
T9943 |
353-358 |
JJ |
denotes |
viral |
T9944 |
359-365 |
NN |
denotes |
vector |
T9945 |
366-369 |
CC |
denotes |
and |
T9946 |
370-376 |
JJ |
denotes |
NuLi-1 |
T9947 |
377-382 |
NNS |
denotes |
cells |
T9948 |
383-387 |
VBD |
denotes |
were |
T9949 |
388-395 |
VBN |
denotes |
derived |
T9950 |
396-400 |
IN |
denotes |
from |
T9951 |
401-402 |
DT |
denotes |
a |
T9952 |
403-410 |
JJ |
denotes |
patient |
T9953 |
411-421 |
VBG |
denotes |
possessing |
T9954 |
422-423 |
DT |
denotes |
a |
T9955 |
424-433 |
JJ |
denotes |
wild-type |
T9956 |
434-438 |
NNP |
denotes |
CFTR |
T9957 |
439-447 |
NN |
denotes |
genotype |
T9958 |
447-448 |
. |
denotes |
. |
T9959 |
449-459 |
JJ |
denotes |
THP1-XBlue |
T9960 |
460-465 |
NNS |
denotes |
cells |
T9961 |
466-472 |
RB |
denotes |
stably |
T9962 |
473-480 |
VBP |
denotes |
express |
T9963 |
481-482 |
DT |
denotes |
a |
T9964 |
483-491 |
JJ |
denotes |
secreted |
T9965 |
492-501 |
JJ |
denotes |
embryonic |
T9966 |
502-510 |
NN |
denotes |
alkaline |
T9967 |
511-522 |
NN |
denotes |
phosphatase |
T9968 |
523-524 |
-LRB- |
denotes |
( |
T9969 |
524-528 |
NNP |
denotes |
SEAP |
T9970 |
528-529 |
-RRB- |
denotes |
) |
T9971 |
530-538 |
NN |
denotes |
reporter |
T9972 |
539-548 |
JJ |
denotes |
inducible |
T9973 |
549-551 |
IN |
denotes |
by |
T9974 |
552-557 |
NNP |
denotes |
NF-κB |
T9975 |
558-561 |
CC |
denotes |
and |
T9976 |
562-566 |
NNP |
denotes |
AP-1 |
T9977 |
567-568 |
-LRB- |
denotes |
( |
T9978 |
568-577 |
NNP |
denotes |
Invivogen |
T9979 |
577-578 |
-RRB- |
denotes |
) |
T9980 |
578-579 |
. |
denotes |
. |
T9981 |
580-585 |
NNS |
denotes |
Cells |
T9982 |
586-590 |
VBD |
denotes |
were |
T9983 |
591-599 |
VBN |
denotes |
cultured |
T9984 |
600-602 |
IN |
denotes |
as |
T9985 |
603-614 |
VBN |
denotes |
recommended |
T9986 |
615-617 |
IN |
denotes |
by |
T9987 |
618-623 |
PRP$ |
denotes |
their |
T9988 |
624-634 |
JJ |
denotes |
respective |
T9989 |
635-644 |
NNS |
denotes |
suppliers |
T9990 |
645-650 |
VBG |
denotes |
using |
T9991 |
651-659 |
JJ |
denotes |
standard |
T9992 |
660-669 |
NNS |
denotes |
protocols |
T9993 |
669-670 |
. |
denotes |
. |
T9994 |
671-673 |
CD |
denotes |
S9 |
T9995 |
674-677 |
CC |
denotes |
and |
T9996 |
678-683 |
CD |
denotes |
IB3-1 |
T9997 |
684-689 |
NNS |
denotes |
cells |
T9998 |
690-694 |
VBD |
denotes |
were |
T9999 |
695-703 |
VBN |
denotes |
cultured |
T10000 |
704-706 |
IN |
denotes |
in |
T10001 |
707-712 |
JJ |
denotes |
basal |
T10002 |
713-718 |
NN |
denotes |
LHC-8 |
T10003 |
719-720 |
-LRB- |
denotes |
( |
T10004 |
720-730 |
NNP |
denotes |
Invitrogen |
T10005 |
730-731 |
-RRB- |
denotes |
) |
T10006 |
732-744 |
VBN |
denotes |
supplemented |
T10007 |
745-749 |
IN |
denotes |
with |
T10008 |
750-752 |
CD |
denotes |
10 |
T10009 |
752-753 |
NN |
denotes |
% |
T10010 |
754-755 |
-LRB- |
denotes |
( |
T10011 |
755-758 |
FW |
denotes |
v/v |
T10012 |
758-759 |
-RRB- |
denotes |
) |
T10013 |
760-765 |
JJ |
denotes |
fetal |
T10014 |
766-772 |
JJ |
denotes |
bovine |
T10015 |
773-778 |
NN |
denotes |
serum |
T10016 |
779-780 |
-LRB- |
denotes |
( |
T10017 |
780-783 |
NNP |
denotes |
FBS |
T10018 |
783-784 |
-RRB- |
denotes |
) |
T10019 |
784-785 |
, |
denotes |
, |
T10020 |
786-787 |
CD |
denotes |
2 |
T10021 |
788-790 |
NNP |
denotes |
mM |
T10022 |
791-802 |
NNP |
denotes |
L-glutamine |
T10023 |
802-803 |
, |
denotes |
, |
T10024 |
804-807 |
CC |
denotes |
and |
T10025 |
808-809 |
CD |
denotes |
1 |
T10026 |
810-812 |
NNP |
denotes |
mM |
T10027 |
813-819 |
NN |
denotes |
sodium |
T10028 |
820-828 |
NN |
denotes |
pyruvate |
T10029 |
828-829 |
. |
denotes |
. |
T10030 |
830-836 |
JJ |
denotes |
NuLi-1 |
T10031 |
836-837 |
NN |
denotes |
/ |
T10032 |
837-843 |
NN |
denotes |
CuFi-1 |
T10033 |
844-849 |
NNS |
denotes |
cells |
T10034 |
850-854 |
VBD |
denotes |
were |
T10035 |
855-863 |
VBN |
denotes |
cultured |
T10036 |
864-866 |
IN |
denotes |
in |
T10037 |
867-871 |
NNP |
denotes |
BEBM |
T10038 |
872-882 |
JJ |
denotes |
serum-free |
T10039 |
883-889 |
NN |
denotes |
medium |
T10040 |
890-891 |
-LRB- |
denotes |
( |
T10041 |
891-896 |
NNP |
denotes |
Lonza |
T10042 |
896-897 |
-RRB- |
denotes |
) |
T10043 |
898-902 |
IN |
denotes |
with |
T10044 |
903-913 |
NN |
denotes |
supplement |
T10045 |
914-920 |
NN |
denotes |
bullet |
T10046 |
921-924 |
NN |
denotes |
kit |
T10047 |
925-926 |
-LRB- |
denotes |
( |
T10048 |
926-929 |
NNP |
denotes |
EGF |
T10049 |
929-930 |
, |
denotes |
, |
T10050 |
931-945 |
NN |
denotes |
hydrocortisone |
T10051 |
945-946 |
, |
denotes |
, |
T10052 |
947-953 |
JJ |
denotes |
bovine |
T10053 |
954-963 |
JJ |
denotes |
pituitary |
T10054 |
964-971 |
VB |
denotes |
extract |
T10055 |
971-972 |
, |
denotes |
, |
T10056 |
973-984 |
NN |
denotes |
transferrin |
T10057 |
984-985 |
, |
denotes |
, |
T10058 |
986-992 |
JJ |
denotes |
bovine |
T10059 |
993-1000 |
NN |
denotes |
insulin |
T10060 |
1000-1001 |
, |
denotes |
, |
T10061 |
1002-1018 |
NN |
denotes |
triiodothyronine |
T10062 |
1018-1019 |
, |
denotes |
, |
T10063 |
1020-1031 |
NN |
denotes |
epinephrine |
T10064 |
1031-1032 |
, |
denotes |
, |
T10065 |
1033-1041 |
JJ |
denotes |
retinoic |
T10066 |
1042-1046 |
NN |
denotes |
acid |
T10067 |
1046-1047 |
-RRB- |
denotes |
) |
T10068 |
1047-1048 |
, |
denotes |
, |
T10069 |
1049-1050 |
CD |
denotes |
2 |
T10070 |
1051-1053 |
NNP |
denotes |
mM |
T10071 |
1054-1065 |
NNP |
denotes |
L-glutamine |
T10072 |
1065-1066 |
, |
denotes |
, |
T10073 |
1067-1070 |
CC |
denotes |
and |
T10074 |
1071-1072 |
CD |
denotes |
1 |
T10075 |
1073-1075 |
NNP |
denotes |
mM |
T10076 |
1076-1082 |
NN |
denotes |
sodium |
T10077 |
1083-1091 |
NN |
denotes |
pyruvate |
T10078 |
1091-1092 |
. |
denotes |
. |
T10079 |
1093-1098 |
NNS |
denotes |
PBMCs |
T10080 |
1099-1103 |
IN |
denotes |
from |
T10081 |
1104-1106 |
NNP |
denotes |
CF |
T10082 |
1107-1115 |
NNS |
denotes |
patients |
T10083 |
1116-1119 |
CC |
denotes |
and |
T10084 |
1120-1128 |
NNS |
denotes |
controls |
T10085 |
1129-1133 |
VBD |
denotes |
were |
T10086 |
1134-1142 |
VBN |
denotes |
cultured |
T10087 |
1143-1145 |
IN |
denotes |
in |
T10088 |
1146-1155 |
NNP |
denotes |
RPMI-1640 |
T10089 |
1156-1157 |
-LRB- |
denotes |
( |
T10090 |
1157-1164 |
NNP |
denotes |
Hyclone |
T10091 |
1164-1165 |
-RRB- |
denotes |
) |
T10092 |
1166-1178 |
VBN |
denotes |
supplemented |
T10093 |
1179-1183 |
IN |
denotes |
with |
T10094 |
1184-1186 |
CD |
denotes |
10 |
T10095 |
1186-1187 |
NN |
denotes |
% |
T10096 |
1188-1191 |
NNP |
denotes |
FBS |
T10097 |
1191-1192 |
, |
denotes |
, |
T10098 |
1193-1194 |
CD |
denotes |
2 |
T10099 |
1195-1197 |
NNP |
denotes |
mM |
T10100 |
1198-1209 |
NNP |
denotes |
L-glutamine |
T10101 |
1209-1210 |
, |
denotes |
, |
T10102 |
1211-1214 |
CC |
denotes |
and |
T10103 |
1215-1216 |
CD |
denotes |
1 |
T10104 |
1217-1219 |
NNP |
denotes |
mM |
T10105 |
1220-1226 |
NN |
denotes |
sodium |
T10106 |
1227-1235 |
NN |
denotes |
pyruvate |
T10107 |
1236-1237 |
-LRB- |
denotes |
( |
T10108 |
1237-1245 |
JJ |
denotes |
complete |
T10109 |
1246-1250 |
NNP |
denotes |
RPMI |
T10110 |
1250-1251 |
-RRB- |
denotes |
) |
T10111 |
1251-1252 |
. |
denotes |
. |
T10112 |
1253-1263 |
JJ |
denotes |
THP1-XBlue |
T10113 |
1264-1269 |
NNS |
denotes |
cells |
T10114 |
1270-1274 |
VBD |
denotes |
were |
T10115 |
1275-1283 |
VBN |
denotes |
cultured |
T10116 |
1284-1286 |
IN |
denotes |
in |
T10117 |
1287-1295 |
JJ |
denotes |
complete |
T10118 |
1296-1300 |
NNP |
denotes |
RPMI |
T10119 |
1301-1305 |
IN |
denotes |
with |
T10120 |
1306-1309 |
DT |
denotes |
the |
T10121 |
1310-1318 |
NN |
denotes |
addition |
T10122 |
1319-1321 |
IN |
denotes |
of |
T10123 |
1322-1328 |
NN |
denotes |
zeocin |
T10124 |
1329-1330 |
-LRB- |
denotes |
( |
T10125 |
1330-1333 |
CD |
denotes |
100 |
T10126 |
1334-1336 |
NN |
denotes |
µg |
T10127 |
1336-1337 |
NN |
denotes |
/ |
T10128 |
1337-1339 |
NN |
denotes |
ml |
T10129 |
1339-1340 |
-RRB- |
denotes |
) |
T10130 |
1341-1343 |
TO |
denotes |
to |
T10131 |
1344-1350 |
VB |
denotes |
select |
T10132 |
1351-1354 |
IN |
denotes |
for |
T10133 |
1355-1360 |
NNS |
denotes |
cells |
T10134 |
1361-1371 |
VBG |
denotes |
expressing |
T10135 |
1372-1375 |
DT |
denotes |
the |
T10136 |
1376-1380 |
NNP |
denotes |
SEAP |
T10137 |
1381-1386 |
NNP |
denotes |
NF-κB |
T10138 |
1386-1387 |
NNP |
denotes |
/ |
T10139 |
1387-1391 |
NNP |
denotes |
AP-1 |
T10140 |
1392-1400 |
NN |
denotes |
reporter |
T10141 |
1400-1401 |
. |
denotes |
. |
T10142 |
1402-1407 |
RB |
denotes |
Prior |
T10143 |
1408-1410 |
TO |
denotes |
to |
T10144 |
1411-1422 |
NN |
denotes |
stimulation |
T10145 |
1422-1423 |
, |
denotes |
, |
T10146 |
1424-1433 |
JJ |
denotes |
bronchial |
T10147 |
1434-1444 |
JJ |
denotes |
epithelial |
T10148 |
1445-1449 |
NN |
denotes |
cell |
T10149 |
1450-1455 |
NNS |
denotes |
lines |
T10150 |
1456-1460 |
VBD |
denotes |
were |
T10151 |
1461-1467 |
VBN |
denotes |
plated |
T10152 |
1468-1470 |
IN |
denotes |
in |
T10153 |
1471-1477 |
JJ |
denotes |
coated |
T10154 |
1478-1479 |
NNP |
denotes |
[ |
T10155 |
1479-1481 |
CD |
denotes |
62 |
T10156 |
1481-1482 |
NNP |
denotes |
] |
T10157 |
1483-1490 |
JJ |
denotes |
96-well |
T10158 |
1491-1497 |
NNS |
denotes |
plates |
T10159 |
1498-1499 |
-LRB- |
denotes |
( |
T10160 |
1499-1501 |
NNP |
denotes |
BD |
T10161 |
1502-1513 |
NNPS |
denotes |
Biosciences |
T10162 |
1513-1514 |
-RRB- |
denotes |
) |
T10163 |
1515-1517 |
IN |
denotes |
at |
T10164 |
1518-1519 |
CD |
denotes |
3 |
T10165 |
1519-1520 |
CD |
denotes |
× |
T10166 |
1520-1523 |
CD |
denotes |
104 |
T10167 |
1524-1534 |
NN |
denotes |
cells/well |
T10168 |
1535-1541 |
IN |
denotes |
unless |
T10169 |
1542-1551 |
VBN |
denotes |
indicated |
T10170 |
1551-1552 |
, |
denotes |
, |
T10171 |
1553-1556 |
CC |
denotes |
and |
T10172 |
1557-1564 |
VBD |
denotes |
allowed |
T10173 |
1565-1567 |
TO |
denotes |
to |
T10174 |
1568-1574 |
VB |
denotes |
adhere |
T10175 |
1575-1584 |
JJ |
denotes |
overnight |
T10176 |
1584-1585 |
. |
denotes |
. |
T10177 |
1586-1592 |
NNP |
denotes |
Plates |
T10178 |
1593-1596 |
IN |
denotes |
for |
T10179 |
1597-1599 |
NNP |
denotes |
S9 |
T10180 |
1600-1603 |
CC |
denotes |
and |
T10181 |
1604-1609 |
NNP |
denotes |
IB3-1 |
T10182 |
1610-1622 |
NNS |
denotes |
stimulations |
T10183 |
1623-1627 |
VBD |
denotes |
were |
T10184 |
1628-1634 |
VBN |
denotes |
coated |
T10185 |
1635-1637 |
IN |
denotes |
in |
T10186 |
1638-1639 |
DT |
denotes |
a |
T10187 |
1640-1647 |
NN |
denotes |
mixture |
T10188 |
1648-1650 |
IN |
denotes |
of |
T10189 |
1651-1657 |
JJ |
denotes |
bovine |
T10190 |
1658-1663 |
NN |
denotes |
serum |
T10191 |
1664-1671 |
NN |
denotes |
albumin |
T10192 |
1672-1673 |
-LRB- |
denotes |
( |
T10193 |
1673-1676 |
CD |
denotes |
100 |
T10194 |
1677-1679 |
NN |
denotes |
µg |
T10195 |
1679-1680 |
NN |
denotes |
/ |
T10196 |
1680-1682 |
NN |
denotes |
ml |
T10197 |
1682-1683 |
-RRB- |
denotes |
) |
T10198 |
1683-1684 |
, |
denotes |
, |
T10199 |
1685-1696 |
NN |
denotes |
fibronectin |
T10200 |
1697-1698 |
-LRB- |
denotes |
( |
T10201 |
1698-1700 |
CD |
denotes |
10 |
T10202 |
1701-1703 |
NN |
denotes |
µg |
T10203 |
1703-1704 |
NN |
denotes |
/ |
T10204 |
1704-1706 |
NN |
denotes |
ml |
T10205 |
1706-1707 |
-RRB- |
denotes |
) |
T10206 |
1707-1708 |
, |
denotes |
, |
T10207 |
1709-1712 |
CC |
denotes |
and |
T10208 |
1713-1719 |
JJ |
denotes |
bovine |
T10209 |
1720-1727 |
NN |
denotes |
collage |
T10210 |
1728-1732 |
NN |
denotes |
type |
T10211 |
1733-1734 |
PRP |
denotes |
I |
T10212 |
1735-1736 |
-LRB- |
denotes |
( |
T10213 |
1736-1738 |
CD |
denotes |
30 |
T10214 |
1739-1741 |
NN |
denotes |
µg |
T10215 |
1741-1742 |
NN |
denotes |
/ |
T10216 |
1742-1744 |
NN |
denotes |
ml |
T10217 |
1744-1745 |
-RRB- |
denotes |
) |
T10218 |
1746-1747 |
-LRB- |
denotes |
( |
T10219 |
1747-1749 |
NNP |
denotes |
BD |
T10220 |
1750-1761 |
NNPS |
denotes |
Biosciences |
T10221 |
1761-1762 |
-RRB- |
denotes |
) |
T10222 |
1762-1763 |
. |
denotes |
. |
T10223 |
1764-1770 |
NNS |
denotes |
Plates |
T10224 |
1771-1774 |
IN |
denotes |
for |
T10225 |
1775-1781 |
NN |
denotes |
NuLi-1 |
T10226 |
1782-1785 |
CC |
denotes |
and |
T10227 |
1786-1792 |
NN |
denotes |
CuFi-1 |
T10228 |
1793-1797 |
VBD |
denotes |
were |
T10229 |
1798-1804 |
VBN |
denotes |
coated |
T10230 |
1805-1809 |
IN |
denotes |
with |
T10231 |
1810-1818 |
NN |
denotes |
collagen |
T10232 |
1819-1823 |
NN |
denotes |
type |
T10233 |
1824-1826 |
NNP |
denotes |
IV |
T10234 |
1827-1828 |
-LRB- |
denotes |
( |
T10235 |
1828-1830 |
CD |
denotes |
60 |
T10236 |
1831-1833 |
NN |
denotes |
µg |
T10237 |
1833-1834 |
NN |
denotes |
/ |
T10238 |
1834-1836 |
NN |
denotes |
ml |
T10239 |
1836-1837 |
-RRB- |
denotes |
) |
T10240 |
1838-1839 |
-LRB- |
denotes |
( |
T10241 |
1839-1844 |
NNP |
denotes |
Sigma |
T10242 |
1845-1852 |
NNP |
denotes |
Aldrich |
T10243 |
1852-1853 |
-RRB- |
denotes |
) |
T10244 |
1853-1854 |
. |
denotes |
. |
T10245 |
1855-1860 |
NNS |
denotes |
PBMCs |
T10246 |
1861-1865 |
VBD |
denotes |
were |
T10247 |
1866-1872 |
VBN |
denotes |
plated |
T10248 |
1873-1875 |
IN |
denotes |
in |
T10249 |
1876-1883 |
JJ |
denotes |
96-well |
T10250 |
1884-1890 |
NNS |
denotes |
plates |
T10251 |
1891-1893 |
IN |
denotes |
at |
T10252 |
1894-1895 |
DT |
denotes |
a |
T10253 |
1896-1903 |
NN |
denotes |
density |
T10254 |
1904-1906 |
IN |
denotes |
of |
T10255 |
1907-1910 |
CD |
denotes |
1.5 |
T10256 |
1910-1911 |
CD |
denotes |
× |
T10257 |
1911-1914 |
CD |
denotes |
105 |
T10258 |
1915-1925 |
NN |
denotes |
cells/well |
T10259 |
1926-1928 |
IN |
denotes |
in |
T10260 |
1929-1932 |
CD |
denotes |
200 |
T10261 |
1933-1935 |
NN |
denotes |
µl |
T10262 |
1936-1937 |
-LRB- |
denotes |
( |
T10263 |
1937-1940 |
CD |
denotes |
7.5 |
T10264 |
1940-1941 |
CD |
denotes |
× |
T10265 |
1941-1944 |
CD |
denotes |
105 |
T10266 |
1945-1953 |
NNP |
denotes |
cells/mL |
T10267 |
1953-1954 |
-RRB- |
denotes |
) |
T10268 |
1954-1955 |
. |
denotes |
. |
T10269 |
1956-1961 |
CD |
denotes |
THP-1 |
T10270 |
1962-1970 |
NN |
denotes |
reporter |
T10271 |
1971-1976 |
NNS |
denotes |
cells |
T10272 |
1977-1981 |
VBD |
denotes |
were |
T10273 |
1982-1996 |
VBN |
denotes |
differentiated |
T10274 |
1997-2001 |
IN |
denotes |
into |
T10275 |
2002-2003 |
DT |
denotes |
a |
T10276 |
2004-2019 |
JJ |
denotes |
macrophage-like |
T10277 |
2020-2029 |
NN |
denotes |
phenotype |
T10278 |
2030-2035 |
VBG |
denotes |
using |
T10279 |
2036-2038 |
CD |
denotes |
50 |
T10280 |
2039-2044 |
NN |
denotes |
ng/ml |
T10281 |
2045-2047 |
IN |
denotes |
of |
T10282 |
2048-2055 |
NN |
denotes |
phorbol |
T10283 |
2056-2068 |
JJ |
denotes |
12-myristate |
T10284 |
2069-2079 |
JJ |
denotes |
13-acetate |
T10285 |
2080-2081 |
-LRB- |
denotes |
( |
T10286 |
2081-2084 |
NNP |
denotes |
PMA |
T10287 |
2084-2085 |
-RRB- |
denotes |
) |
T10288 |
2086-2087 |
-LRB- |
denotes |
( |
T10289 |
2087-2092 |
NNP |
denotes |
Sigma |
T10290 |
2093-2100 |
NNP |
denotes |
Aldrich |
T10291 |
2100-2101 |
-RRB- |
denotes |
) |
T10292 |
2102-2105 |
IN |
denotes |
for |
T10293 |
2106-2108 |
CD |
denotes |
24 |
T10294 |
2109-2114 |
NNS |
denotes |
hours |
T10295 |
2115-2117 |
IN |
denotes |
at |
T10296 |
2118-2119 |
DT |
denotes |
a |
T10297 |
2120-2127 |
NN |
denotes |
density |
T10298 |
2128-2130 |
IN |
denotes |
of |
T10299 |
2131-2132 |
CD |
denotes |
1 |
T10300 |
2132-2133 |
CD |
denotes |
× |
T10301 |
2133-2136 |
CD |
denotes |
105 |
T10302 |
2137-2147 |
NN |
denotes |
cells/well |
T10303 |
2148-2150 |
IN |
denotes |
in |
T10304 |
2151-2154 |
CD |
denotes |
200 |
T10305 |
2155-2157 |
NN |
denotes |
µl |
T10306 |
2158-2159 |
-LRB- |
denotes |
( |
T10307 |
2159-2160 |
CD |
denotes |
5 |
T10308 |
2160-2161 |
CD |
denotes |
× |
T10309 |
2161-2164 |
CD |
denotes |
105 |
T10310 |
2165-2173 |
NN |
denotes |
cells/ml |
T10311 |
2173-2174 |
-RRB- |
denotes |
) |
T10312 |
2174-2175 |
. |
denotes |
. |
T10313 |
2176-2181 |
NNS |
denotes |
Cells |
T10314 |
2182-2186 |
VBD |
denotes |
were |
T10315 |
2187-2193 |
VBN |
denotes |
washed |
T10316 |
2194-2198 |
IN |
denotes |
with |
T10317 |
2199-2202 |
NNP |
denotes |
PBS |
T10318 |
2203-2206 |
CC |
denotes |
and |
T10319 |
2207-2214 |
VBN |
denotes |
allowed |
T10320 |
2215-2217 |
TO |
denotes |
to |
T10321 |
2218-2222 |
VB |
denotes |
rest |
T10322 |
2223-2224 |
DT |
denotes |
a |
T10323 |
2225-2232 |
JJ |
denotes |
further |
T10324 |
2233-2235 |
CD |
denotes |
42 |
T10325 |
2236-2241 |
NNS |
denotes |
hours |
T10326 |
2242-2247 |
RB |
denotes |
prior |
T10327 |
2248-2250 |
TO |
denotes |
to |
T10328 |
2251-2262 |
NN |
denotes |
stimulation |
T10329 |
2262-2263 |
. |
denotes |
. |
R8670 |
T9881 |
T9883 |
compound |
Cell,CF |
R8671 |
T9882 |
T9883 |
compound |
Culture,CF |
R8672 |
T9883 |
T9898 |
nsubjpass |
CF,obtained |
R8673 |
T9884 |
T9883 |
punct |
(,CF |
R8674 |
T9885 |
T9896 |
nmod |
IB3-1,cells |
R8675 |
T9886 |
T9885 |
cc |
and,IB3-1 |
R8676 |
T9887 |
T9885 |
conj |
CuFi-1,IB3-1 |
R8677 |
T9888 |
T9887 |
punct |
),CuFi-1 |
R8678 |
T9889 |
T9885 |
cc |
and,IB3-1 |
R8679 |
T9890 |
T9885 |
conj |
control,IB3-1 |
R8680 |
T9891 |
T9890 |
punct |
(,control |
R8681 |
T9892 |
T9890 |
appos |
S9,control |
R8682 |
T9893 |
T9885 |
cc |
and,IB3-1 |
R8683 |
T9894 |
T9885 |
conj |
NuLi-1,IB3-1 |
R8684 |
T9895 |
T9896 |
punct |
),cells |
R8685 |
T9896 |
T9898 |
nsubjpass |
cells,obtained |
R8686 |
T9897 |
T9898 |
auxpass |
were,obtained |
R8687 |
T9898 |
T9898 |
ROOT |
obtained,obtained |
R8688 |
T9899 |
T9898 |
prep |
from,obtained |
R8689 |
T9900 |
T9904 |
det |
the,Collection |
R8690 |
T9901 |
T9904 |
compound |
American,Collection |
R8691 |
T9902 |
T9904 |
compound |
Type,Collection |
R8692 |
T9903 |
T9904 |
compound |
Culture,Collection |
R8693 |
T9904 |
T9899 |
pobj |
Collection,from |
R8694 |
T9905 |
T9898 |
punct |
.,obtained |
R8695 |
T9906 |
T9907 |
nummod |
IB3-1,cells |
R8696 |
T9907 |
T9909 |
nsubjpass |
cells,derived |
R8697 |
T9908 |
T9909 |
auxpass |
were,derived |
R8698 |
T9909 |
T9909 |
ROOT |
derived,derived |
R8699 |
T9910 |
T9909 |
prep |
from,derived |
R8700 |
T9911 |
T9912 |
det |
a,patient |
R8701 |
T9912 |
T9910 |
pobj |
patient,from |
R8702 |
T9913 |
T9912 |
acl |
expressing,patient |
R8703 |
T9914 |
T9915 |
det |
the,ΔF508 |
R8704 |
T9915 |
T9913 |
dobj |
ΔF508,expressing |
R8705 |
T9916 |
T9915 |
cc |
and,ΔF508 |
R8706 |
T9917 |
T9915 |
conj |
W1282X,ΔF508 |
R8707 |
T9918 |
T9915 |
conj |
mutations,ΔF508 |
R8708 |
T9919 |
T9918 |
cc |
and,mutations |
R8709 |
T9920 |
T9918 |
conj |
CuFi-1,mutations |
R8710 |
T9921 |
T9922 |
auxpass |
were,derived |
R8711 |
T9922 |
T9909 |
conj |
derived,derived |
R8712 |
T9923 |
T9922 |
prep |
from,derived |
R8713 |
T9924 |
T9927 |
det |
a,patient |
R8714 |
T9925 |
T9927 |
nmod |
ΔF508,patient |
R8715 |
T9926 |
T9927 |
amod |
homozygous,patient |
R8716 |
T9927 |
T9923 |
pobj |
patient,from |
R8717 |
T9928 |
T9909 |
punct |
.,derived |
R8718 |
T9929 |
T9930 |
nummod |
S9,cells |
R8719 |
T9930 |
T9931 |
nsubj |
cells,are |
R8720 |
T9931 |
T9931 |
ROOT |
are,are |
R8721 |
T9932 |
T9933 |
amod |
IB3-1,cells |
R8722 |
T9933 |
T9931 |
attr |
cells,are |
R8723 |
T9934 |
T9937 |
nsubjpass |
that,transfected |
R8724 |
T9935 |
T9937 |
aux |
have,transfected |
R8725 |
T9936 |
T9937 |
auxpass |
been,transfected |
R8726 |
T9937 |
T9933 |
relcl |
transfected,cells |
R8727 |
T9938 |
T9937 |
prep |
with,transfected |
R8728 |
T9939 |
T9940 |
nsubj |
CFTR,using |
R8729 |
T9940 |
T9938 |
pcomp |
using,with |
R8730 |
T9941 |
T9949 |
det |
an,derived |
R8731 |
T9942 |
T9944 |
amod |
adeno-associated,vector |
R8732 |
T9943 |
T9944 |
amod |
viral,vector |
R8733 |
T9944 |
T9949 |
nsubjpass |
vector,derived |
R8734 |
T9945 |
T9944 |
cc |
and,vector |
R8735 |
T9946 |
T9944 |
conj |
NuLi-1,vector |
R8736 |
T9947 |
T9944 |
conj |
cells,vector |
R8737 |
T9948 |
T9949 |
auxpass |
were,derived |
R8738 |
T9949 |
T9931 |
advcl |
derived,are |
R8739 |
T9950 |
T9949 |
prep |
from,derived |
R8740 |
T9951 |
T9952 |
det |
a,patient |
R8741 |
T9952 |
T9950 |
pobj |
patient,from |
R8742 |
T9953 |
T9952 |
acl |
possessing,patient |
R8743 |
T9954 |
T9957 |
det |
a,genotype |
R8744 |
T9955 |
T9957 |
amod |
wild-type,genotype |
R8745 |
T9956 |
T9957 |
amod |
CFTR,genotype |
R8746 |
T9957 |
T9953 |
dobj |
genotype,possessing |
R8747 |
T9958 |
T9949 |
punct |
.,derived |
R8748 |
T9959 |
T9960 |
amod |
THP1-XBlue,cells |
R8749 |
T9960 |
T9962 |
nsubj |
cells,express |
R8750 |
T9961 |
T9962 |
advmod |
stably,express |
R8751 |
T9962 |
T9962 |
ROOT |
express,express |
R8752 |
T9963 |
T9967 |
det |
a,phosphatase |
R8753 |
T9964 |
T9967 |
amod |
secreted,phosphatase |
R8754 |
T9965 |
T9967 |
amod |
embryonic,phosphatase |
R8755 |
T9966 |
T9967 |
compound |
alkaline,phosphatase |
R8756 |
T9967 |
T9962 |
dobj |
phosphatase,express |
R8757 |
T9968 |
T9967 |
punct |
(,phosphatase |
R8758 |
T9969 |
T9967 |
appos |
SEAP,phosphatase |
R8759 |
T9970 |
T9967 |
punct |
),phosphatase |
R8760 |
T9971 |
T9972 |
compound |
reporter,inducible |
R8761 |
T9972 |
T9967 |
appos |
inducible,phosphatase |
R8762 |
T9973 |
T9972 |
prep |
by,inducible |
R8763 |
T9974 |
T9973 |
pobj |
NF-κB,by |
R8764 |
T9975 |
T9974 |
cc |
and,NF-κB |
R8765 |
T9976 |
T9974 |
conj |
AP-1,NF-κB |
R8766 |
T9977 |
T9978 |
punct |
(,Invivogen |
R8767 |
T9978 |
T9976 |
appos |
Invivogen,AP-1 |
R8768 |
T9979 |
T9976 |
punct |
),AP-1 |
R8769 |
T9980 |
T9962 |
punct |
.,express |
R8770 |
T9981 |
T9983 |
nsubjpass |
Cells,cultured |
R8771 |
T9982 |
T9983 |
auxpass |
were,cultured |
R8772 |
T9983 |
T9983 |
ROOT |
cultured,cultured |
R8773 |
T9984 |
T9985 |
mark |
as,recommended |
R8774 |
T9985 |
T9983 |
advcl |
recommended,cultured |
R8775 |
T9986 |
T9985 |
agent |
by,recommended |
R8776 |
T9987 |
T9989 |
poss |
their,suppliers |
R8777 |
T9988 |
T9989 |
amod |
respective,suppliers |
R8778 |
T9989 |
T9986 |
pobj |
suppliers,by |
R8779 |
T9990 |
T9989 |
acl |
using,suppliers |
R8780 |
T9991 |
T9992 |
amod |
standard,protocols |
R8781 |
T9992 |
T9990 |
dobj |
protocols,using |
R8782 |
T9993 |
T9983 |
punct |
.,cultured |
R8783 |
T9994 |
T9997 |
nummod |
S9,cells |
R8784 |
T9995 |
T9994 |
cc |
and,S9 |
R8785 |
T9996 |
T9994 |
conj |
IB3-1,S9 |
R8786 |
T9997 |
T9999 |
nsubjpass |
cells,cultured |
R8787 |
T9998 |
T9999 |
auxpass |
were,cultured |
R8788 |
T9999 |
T9999 |
ROOT |
cultured,cultured |
R8789 |
T10000 |
T9999 |
prep |
in,cultured |
R8790 |
T10001 |
T10002 |
amod |
basal,LHC-8 |
R8791 |
T10002 |
T10000 |
pobj |
LHC-8,in |
R8792 |
T10003 |
T10004 |
punct |
(,Invitrogen |
R8793 |
T10004 |
T10002 |
appos |
Invitrogen,LHC-8 |
R8794 |
T10005 |
T9999 |
punct |
),cultured |
R8795 |
T10006 |
T9999 |
prep |
supplemented,cultured |
R8796 |
T10007 |
T10006 |
prep |
with,supplemented |
R8797 |
T10008 |
T10009 |
nummod |
10,% |
R8798 |
T10009 |
T10007 |
pobj |
%,with |
R8799 |
T10010 |
T10011 |
punct |
(,v/v |
R8800 |
T10011 |
T10009 |
appos |
v/v,% |
R8801 |
T10012 |
T10011 |
punct |
),v/v |
R8802 |
T10013 |
T10015 |
amod |
fetal,serum |
R8803 |
T10014 |
T10015 |
amod |
bovine,serum |
R8804 |
T10015 |
T10009 |
appos |
serum,% |
R8805 |
T10016 |
T10017 |
punct |
(,FBS |
R8806 |
T10017 |
T10015 |
parataxis |
FBS,serum |
R8807 |
T10018 |
T10017 |
punct |
),FBS |
R8808 |
T10019 |
T10015 |
punct |
",",serum |
R8809 |
T10020 |
T10022 |
nummod |
2,L-glutamine |
R8810 |
T10021 |
T10022 |
compound |
mM,L-glutamine |
R8811 |
T10022 |
T10015 |
appos |
L-glutamine,serum |
R8812 |
T10023 |
T10015 |
punct |
",",serum |
R8813 |
T10024 |
T10015 |
cc |
and,serum |
R8814 |
T10025 |
T10026 |
nummod |
1,mM |
R8815 |
T10026 |
T10028 |
compound |
mM,pyruvate |
R8816 |
T10027 |
T10028 |
compound |
sodium,pyruvate |
R8817 |
T10028 |
T10015 |
conj |
pyruvate,serum |
R8818 |
T10029 |
T9999 |
punct |
.,cultured |
R8819 |
T10030 |
T10033 |
amod |
NuLi-1,cells |
R8820 |
T10031 |
T10033 |
compound |
/,cells |
R8821 |
T10032 |
T10033 |
compound |
CuFi-1,cells |
R8822 |
T10033 |
T10034 |
nsubj |
cells,were |
R8823 |
T10034 |
T10034 |
ROOT |
were,were |
R8824 |
T10035 |
T10034 |
acomp |
cultured,were |
R8825 |
T10036 |
T10035 |
prep |
in,cultured |
R8826 |
T10037 |
T10039 |
nmod |
BEBM,medium |
R8827 |
T10038 |
T10039 |
amod |
serum-free,medium |
R8828 |
T10039 |
T10036 |
pobj |
medium,in |
R8829 |
T10040 |
T10041 |
punct |
(,Lonza |
R8830 |
T10041 |
T10039 |
appos |
Lonza,medium |
R8831 |
T10042 |
T10035 |
punct |
),cultured |
R8832 |
T10043 |
T10034 |
prep |
with,were |
R8833 |
T10044 |
T10046 |
compound |
supplement,kit |
R8834 |
T10045 |
T10046 |
compound |
bullet,kit |
R8835 |
T10046 |
T10043 |
pobj |
kit,with |
R8836 |
T10047 |
T10046 |
punct |
(,kit |
R8837 |
T10048 |
T10050 |
nmod |
EGF,hydrocortisone |
R8838 |
T10049 |
T10050 |
punct |
",",hydrocortisone |
R8839 |
T10050 |
T10046 |
appos |
hydrocortisone,kit |
R8840 |
T10051 |
T10050 |
punct |
",",hydrocortisone |
R8841 |
T10052 |
T10054 |
amod |
bovine,extract |
R8842 |
T10053 |
T10054 |
amod |
pituitary,extract |
R8843 |
T10054 |
T10050 |
conj |
extract,hydrocortisone |
R8844 |
T10055 |
T10054 |
punct |
",",extract |
R8845 |
T10056 |
T10050 |
conj |
transferrin,hydrocortisone |
R8846 |
T10057 |
T10056 |
punct |
",",transferrin |
R8847 |
T10058 |
T10059 |
amod |
bovine,insulin |
R8848 |
T10059 |
T10056 |
conj |
insulin,transferrin |
R8849 |
T10060 |
T10059 |
punct |
",",insulin |
R8850 |
T10061 |
T10059 |
conj |
triiodothyronine,insulin |
R8851 |
T10062 |
T10061 |
punct |
",",triiodothyronine |
R8852 |
T10063 |
T10061 |
conj |
epinephrine,triiodothyronine |
R8853 |
T10064 |
T10063 |
punct |
",",epinephrine |
R8854 |
T10065 |
T10066 |
amod |
retinoic,acid |
R8855 |
T10066 |
T10063 |
appos |
acid,epinephrine |
R8856 |
T10067 |
T10063 |
punct |
),epinephrine |
R8857 |
T10068 |
T10061 |
punct |
",",triiodothyronine |
R8858 |
T10069 |
T10071 |
nummod |
2,L-glutamine |
R8859 |
T10070 |
T10071 |
compound |
mM,L-glutamine |
R8860 |
T10071 |
T10061 |
conj |
L-glutamine,triiodothyronine |
R8861 |
T10072 |
T10071 |
punct |
",",L-glutamine |
R8862 |
T10073 |
T10071 |
cc |
and,L-glutamine |
R8863 |
T10074 |
T10077 |
nummod |
1,pyruvate |
R8864 |
T10075 |
T10077 |
compound |
mM,pyruvate |
R8865 |
T10076 |
T10077 |
compound |
sodium,pyruvate |
R8866 |
T10077 |
T10071 |
conj |
pyruvate,L-glutamine |
R8867 |
T10078 |
T10077 |
punct |
.,pyruvate |
R8868 |
T10079 |
T10085 |
nsubj |
PBMCs,were |
R8869 |
T10080 |
T10079 |
prep |
from,PBMCs |
R8870 |
T10081 |
T10082 |
compound |
CF,patients |
R8871 |
T10082 |
T10080 |
pobj |
patients,from |
R8872 |
T10083 |
T10082 |
cc |
and,patients |
R8873 |
T10084 |
T10082 |
conj |
controls,patients |
R8874 |
T10085 |
T10085 |
ROOT |
were,were |
R8875 |
T10086 |
T10085 |
acomp |
cultured,were |
R8876 |
T10087 |
T10086 |
prep |
in,cultured |
R8877 |
T10088 |
T10087 |
pobj |
RPMI-1640,in |
R8878 |
T10089 |
T10088 |
punct |
(,RPMI-1640 |
R8879 |
T10090 |
T10088 |
appos |
Hyclone,RPMI-1640 |
R8880 |
T10091 |
T10088 |
punct |
),RPMI-1640 |
R8881 |
T10092 |
T10086 |
conj |
supplemented,cultured |
R8882 |
T10093 |
T10092 |
prep |
with,supplemented |
R8883 |
T10094 |
T10095 |
nummod |
10,% |
R8884 |
T10095 |
T10096 |
compound |
%,FBS |
R8885 |
T10096 |
T10093 |
pobj |
FBS,with |
R8886 |
T10097 |
T10096 |
punct |
",",FBS |
R8887 |
T10098 |
T10100 |
nummod |
2,L-glutamine |
R8888 |
T10099 |
T10100 |
compound |
mM,L-glutamine |
R8889 |
T10100 |
T10096 |
conj |
L-glutamine,FBS |
R8890 |
T10101 |
T10100 |
punct |
",",L-glutamine |
R8891 |
T10102 |
T10100 |
cc |
and,L-glutamine |
R8892 |
T10103 |
T10106 |
nummod |
1,pyruvate |
R8893 |
T10104 |
T10106 |
compound |
mM,pyruvate |
R8894 |
T10105 |
T10106 |
compound |
sodium,pyruvate |
R8895 |
T10106 |
T10100 |
conj |
pyruvate,L-glutamine |
R8896 |
T10107 |
T10106 |
punct |
(,pyruvate |
R8897 |
T10108 |
T10109 |
amod |
complete,RPMI |
R8898 |
T10109 |
T10106 |
appos |
RPMI,pyruvate |
R8899 |
T10110 |
T10106 |
punct |
),pyruvate |
R8900 |
T10111 |
T10085 |
punct |
.,were |
R8901 |
T10112 |
T10113 |
amod |
THP1-XBlue,cells |
R8902 |
T10113 |
T10114 |
nsubj |
cells,were |
R8903 |
T10114 |
T10114 |
ROOT |
were,were |
R8904 |
T10115 |
T10114 |
acomp |
cultured,were |
R8905 |
T10116 |
T10115 |
prep |
in,cultured |
R8906 |
T10117 |
T10118 |
amod |
complete,RPMI |
R8907 |
T10118 |
T10116 |
pobj |
RPMI,in |
R8908 |
T10119 |
T10115 |
prep |
with,cultured |
R8909 |
T10120 |
T10121 |
det |
the,addition |
R8910 |
T10121 |
T10119 |
pobj |
addition,with |
R8911 |
T10122 |
T10121 |
prep |
of,addition |
R8912 |
T10123 |
T10122 |
pobj |
zeocin,of |
R8913 |
T10124 |
T10123 |
punct |
(,zeocin |
R8914 |
T10125 |
T10126 |
nummod |
100,µg |
R8915 |
T10126 |
T10128 |
nmod |
µg,ml |
R8916 |
T10127 |
T10128 |
compound |
/,ml |
R8917 |
T10128 |
T10123 |
appos |
ml,zeocin |
R8918 |
T10129 |
T10128 |
punct |
),ml |
R8919 |
T10130 |
T10131 |
aux |
to,select |
R8920 |
T10131 |
T10115 |
advcl |
select,cultured |
R8921 |
T10132 |
T10131 |
prep |
for,select |
R8922 |
T10133 |
T10132 |
pobj |
cells,for |
R8923 |
T10134 |
T10133 |
acl |
expressing,cells |
R8924 |
T10135 |
T10140 |
det |
the,reporter |
R8925 |
T10136 |
T10139 |
compound |
SEAP,AP-1 |
R8926 |
T10137 |
T10139 |
compound |
NF-κB,AP-1 |
R8927 |
T10138 |
T10139 |
compound |
/,AP-1 |
R8928 |
T10139 |
T10140 |
compound |
AP-1,reporter |
R8929 |
T10140 |
T10134 |
dobj |
reporter,expressing |
R8930 |
T10141 |
T10114 |
punct |
.,were |
R8931 |
T10142 |
T10151 |
advmod |
Prior,plated |
R8932 |
T10143 |
T10151 |
prep |
to,plated |
R8933 |
T10144 |
T10143 |
pobj |
stimulation,to |
R8934 |
T10145 |
T10151 |
punct |
",",plated |
R8935 |
T10146 |
T10149 |
amod |
bronchial,lines |
R8936 |
T10147 |
T10149 |
amod |
epithelial,lines |
R8937 |
T10148 |
T10149 |
compound |
cell,lines |
R8938 |
T10149 |
T10151 |
nsubjpass |
lines,plated |
R8939 |
T10150 |
T10151 |
auxpass |
were,plated |
R8940 |
T10151 |
T10151 |
ROOT |
plated,plated |
R8941 |
T10152 |
T10151 |
prep |
in,plated |
R8942 |
T10153 |
T10158 |
amod |
coated,plates |
R8943 |
T10154 |
T10158 |
nmod |
[,plates |
R8944 |
T10155 |
T10158 |
nummod |
62,plates |
R8945 |
T10156 |
T10158 |
nmod |
],plates |
R8946 |
T10157 |
T10158 |
compound |
96-well,plates |
R8947 |
T10158 |
T10152 |
pobj |
plates,in |
R8948 |
T10159 |
T10161 |
punct |
(,Biosciences |
R8949 |
T10160 |
T10161 |
compound |
BD,Biosciences |
R8950 |
T10161 |
T10158 |
appos |
Biosciences,plates |
R8951 |
T10162 |
T10161 |
punct |
),Biosciences |
R8952 |
T10163 |
T10151 |
prep |
at,plated |
R8953 |
T10164 |
T10167 |
nummod |
3,cells/well |
R8954 |
T10165 |
T10167 |
nummod |
×,cells/well |
R8955 |
T10166 |
T10167 |
nummod |
104,cells/well |
R8956 |
T10167 |
T10163 |
pobj |
cells/well,at |
R8957 |
T10168 |
T10169 |
mark |
unless,indicated |
R8958 |
T10169 |
T10151 |
advcl |
indicated,plated |
R8959 |
T10170 |
T10151 |
punct |
",",plated |
R8960 |
T10171 |
T10151 |
cc |
and,plated |
R8961 |
T10172 |
T10151 |
conj |
allowed,plated |
R8962 |
T10173 |
T10174 |
aux |
to,adhere |
R8963 |
T10174 |
T10172 |
xcomp |
adhere,allowed |
R8964 |
T10175 |
T10174 |
advmod |
overnight,adhere |
R8965 |
T10176 |
T10151 |
punct |
.,plated |
R8966 |
T10177 |
T10184 |
nsubjpass |
Plates,coated |
R8967 |
T10178 |
T10177 |
prep |
for,Plates |
R8968 |
T10179 |
T10178 |
pobj |
S9,for |
R8969 |
T10180 |
T10179 |
cc |
and,S9 |
R8970 |
T10181 |
T10179 |
conj |
IB3-1,S9 |
R8971 |
T10182 |
T10184 |
nsubjpass |
stimulations,coated |
R8972 |
T10183 |
T10184 |
auxpass |
were,coated |
R8973 |
T10184 |
T10184 |
ROOT |
coated,coated |
R8974 |
T10185 |
T10184 |
prep |
in,coated |
R8975 |
T10186 |
T10187 |
det |
a,mixture |
R8976 |
T10187 |
T10185 |
pobj |
mixture,in |
R8977 |
T10188 |
T10187 |
prep |
of,mixture |
R8978 |
T10189 |
T10190 |
amod |
bovine,serum |
R8979 |
T10190 |
T10191 |
compound |
serum,albumin |
R8980 |
T10191 |
T10188 |
pobj |
albumin,of |
R8981 |
T10192 |
T10191 |
punct |
(,albumin |
R8982 |
T10193 |
T10194 |
nummod |
100,µg |
R8983 |
T10194 |
T10196 |
compound |
µg,ml |
R8984 |
T10195 |
T10196 |
compound |
/,ml |
R8985 |
T10196 |
T10191 |
appos |
ml,albumin |
R8986 |
T10197 |
T10191 |
punct |
),albumin |
R8987 |
T10198 |
T10184 |
punct |
",",coated |
R8988 |
T10199 |
T10184 |
dep |
fibronectin,coated |
R8989 |
T10200 |
T10199 |
punct |
(,fibronectin |
R8990 |
T10201 |
T10202 |
nummod |
10,µg |
R8991 |
T10202 |
T10199 |
appos |
µg,fibronectin |
R8992 |
T10203 |
T10204 |
compound |
/,ml |
R8993 |
T10204 |
T10202 |
appos |
ml,µg |
R8994 |
T10205 |
T10202 |
punct |
),µg |
R8995 |
T10206 |
T10199 |
punct |
",",fibronectin |
R8996 |
T10207 |
T10199 |
cc |
and,fibronectin |
R8997 |
T10208 |
T10209 |
amod |
bovine,collage |
R8998 |
T10209 |
T10210 |
compound |
collage,type |
R8999 |
T10210 |
T10199 |
conj |
type,fibronectin |
R9000 |
T10211 |
T10199 |
nsubj |
I,fibronectin |
R9001 |
T10212 |
T10211 |
punct |
(,I |
R9002 |
T10213 |
T10214 |
nummod |
30,µg |
R9003 |
T10214 |
T10216 |
compound |
µg,ml |
R9004 |
T10215 |
T10216 |
compound |
/,ml |
R9005 |
T10216 |
T10211 |
appos |
ml,I |
R9006 |
T10217 |
T10211 |
punct |
),I |
R9007 |
T10218 |
T10220 |
punct |
(,Biosciences |
R9008 |
T10219 |
T10220 |
compound |
BD,Biosciences |
R9009 |
T10220 |
T10211 |
appos |
Biosciences,I |
R9010 |
T10221 |
T10220 |
punct |
),Biosciences |
R9011 |
T10222 |
T10184 |
punct |
.,coated |
R9012 |
T10223 |
T10229 |
nsubjpass |
Plates,coated |
R9013 |
T10224 |
T10223 |
prep |
for,Plates |
R9014 |
T10225 |
T10224 |
pobj |
NuLi-1,for |
R9015 |
T10226 |
T10225 |
cc |
and,NuLi-1 |
R9016 |
T10227 |
T10225 |
conj |
CuFi-1,NuLi-1 |
R9017 |
T10228 |
T10229 |
auxpass |
were,coated |
R9018 |
T10229 |
T10229 |
ROOT |
coated,coated |
R9019 |
T10230 |
T10229 |
prep |
with,coated |
R9020 |
T10231 |
T10232 |
compound |
collagen,type |
R9021 |
T10232 |
T10233 |
compound |
type,IV |
R9022 |
T10233 |
T10230 |
pobj |
IV,with |
R9023 |
T10234 |
T10233 |
punct |
(,IV |
R9024 |
T10235 |
T10236 |
nummod |
60,µg |
R9025 |
T10236 |
T10238 |
compound |
µg,ml |
R9026 |
T10237 |
T10238 |
compound |
/,ml |
R9027 |
T10238 |
T10233 |
appos |
ml,IV |
R9028 |
T10239 |
T10233 |
punct |
),IV |
R9029 |
T10240 |
T10233 |
punct |
(,IV |
R9030 |
T10241 |
T10242 |
compound |
Sigma,Aldrich |
R9031 |
T10242 |
T10233 |
appos |
Aldrich,IV |
R9032 |
T10243 |
T10233 |
punct |
),IV |
R9033 |
T10244 |
T10229 |
punct |
.,coated |
R9034 |
T10245 |
T10247 |
nsubjpass |
PBMCs,plated |
R9035 |
T10246 |
T10247 |
auxpass |
were,plated |
R9036 |
T10247 |
T10247 |
ROOT |
plated,plated |
R9037 |
T10248 |
T10247 |
prep |
in,plated |
R9038 |
T10249 |
T10250 |
amod |
96-well,plates |
R9039 |
T10250 |
T10248 |
pobj |
plates,in |
R9040 |
T10251 |
T10247 |
prep |
at,plated |
R9041 |
T10252 |
T10253 |
det |
a,density |
R9042 |
T10253 |
T10251 |
pobj |
density,at |
R9043 |
T10254 |
T10253 |
prep |
of,density |
R9044 |
T10255 |
T10258 |
nummod |
1.5,cells/well |
R9045 |
T10256 |
T10258 |
nmod |
×,cells/well |
R9046 |
T10257 |
T10258 |
nummod |
105,cells/well |
R9047 |
T10258 |
T10254 |
pobj |
cells/well,of |
R9048 |
T10259 |
T10247 |
prep |
in,plated |
R9049 |
T10260 |
T10261 |
nummod |
200,µl |
R9050 |
T10261 |
T10259 |
pobj |
µl,in |
R9051 |
T10262 |
T10261 |
punct |
(,µl |
R9052 |
T10263 |
T10266 |
nummod |
7.5,cells/mL |
R9053 |
T10264 |
T10266 |
nummod |
×,cells/mL |
R9054 |
T10265 |
T10266 |
nummod |
105,cells/mL |
R9055 |
T10266 |
T10261 |
appos |
cells/mL,µl |
R9056 |
T10267 |
T10261 |
punct |
),µl |
R9057 |
T10268 |
T10247 |
punct |
.,plated |
R9058 |
T10269 |
T10271 |
nummod |
THP-1,cells |
R9059 |
T10270 |
T10271 |
compound |
reporter,cells |
R9060 |
T10271 |
T10273 |
nsubjpass |
cells,differentiated |
R9061 |
T10272 |
T10273 |
auxpass |
were,differentiated |
R9062 |
T10273 |
T10273 |
ROOT |
differentiated,differentiated |
R9063 |
T10274 |
T10273 |
prep |
into,differentiated |
R9064 |
T10275 |
T10277 |
det |
a,phenotype |
R9065 |
T10276 |
T10277 |
amod |
macrophage-like,phenotype |
R9066 |
T10277 |
T10274 |
pobj |
phenotype,into |
R9067 |
T10278 |
T10273 |
advcl |
using,differentiated |
R9068 |
T10279 |
T10280 |
nummod |
50,ng/ml |
R9069 |
T10280 |
T10278 |
dobj |
ng/ml,using |
R9070 |
T10281 |
T10280 |
prep |
of,ng/ml |
R9071 |
T10282 |
T10284 |
nmod |
phorbol,13-acetate |
R9072 |
T10283 |
T10284 |
amod |
12-myristate,13-acetate |
R9073 |
T10284 |
T10281 |
pobj |
13-acetate,of |
R9074 |
T10285 |
T10286 |
punct |
(,PMA |
R9075 |
T10286 |
T10284 |
appos |
PMA,13-acetate |
R9076 |
T10287 |
T10284 |
punct |
),13-acetate |
R9077 |
T10288 |
T10290 |
punct |
(,Aldrich |
R9078 |
T10289 |
T10290 |
compound |
Sigma,Aldrich |
R9079 |
T10290 |
T10284 |
appos |
Aldrich,13-acetate |
R9080 |
T10291 |
T10280 |
punct |
),ng/ml |
R9081 |
T10292 |
T10278 |
prep |
for,using |
R9082 |
T10293 |
T10294 |
nummod |
24,hours |
R9083 |
T10294 |
T10292 |
pobj |
hours,for |
R9084 |
T10295 |
T10278 |
prep |
at,using |
R9085 |
T10296 |
T10297 |
det |
a,density |
R9086 |
T10297 |
T10295 |
pobj |
density,at |
R9087 |
T10298 |
T10297 |
prep |
of,density |
R9088 |
T10299 |
T10302 |
nummod |
1,cells/well |
R9089 |
T10300 |
T10302 |
nummod |
×,cells/well |
R9090 |
T10301 |
T10302 |
nummod |
105,cells/well |
R9091 |
T10302 |
T10298 |
pobj |
cells/well,of |
R9092 |
T10303 |
T10278 |
prep |
in,using |
R9093 |
T10304 |
T10305 |
nummod |
200,µl |
R9094 |
T10305 |
T10303 |
pobj |
µl,in |
R9095 |
T10306 |
T10305 |
punct |
(,µl |
R9096 |
T10307 |
T10310 |
nummod |
5,cells/ml |
R9097 |
T10308 |
T10310 |
nummod |
×,cells/ml |
R9098 |
T10309 |
T10310 |
nummod |
105,cells/ml |
R9099 |
T10310 |
T10305 |
appos |
cells/ml,µl |
R9100 |
T10311 |
T10305 |
punct |
),µl |
R9101 |
T10312 |
T10273 |
punct |
.,differentiated |
R9102 |
T10313 |
T10315 |
nsubjpass |
Cells,washed |
R9103 |
T10314 |
T10315 |
auxpass |
were,washed |
R9104 |
T10315 |
T10315 |
ROOT |
washed,washed |
R9105 |
T10316 |
T10315 |
prep |
with,washed |
R9106 |
T10317 |
T10316 |
pobj |
PBS,with |
R9107 |
T10318 |
T10315 |
cc |
and,washed |
R9108 |
T10319 |
T10315 |
conj |
allowed,washed |
R9109 |
T10320 |
T10321 |
aux |
to,rest |
R9110 |
T10321 |
T10319 |
xcomp |
rest,allowed |
R9111 |
T10322 |
T10326 |
det |
a,prior |
R9112 |
T10323 |
T10326 |
amod |
further,prior |
R9113 |
T10324 |
T10325 |
nummod |
42,hours |
R9114 |
T10325 |
T10326 |
npadvmod |
hours,prior |
R9115 |
T10326 |
T10321 |
advmod |
prior,rest |
R9116 |
T10327 |
T10326 |
prep |
to,prior |
R9117 |
T10328 |
T10327 |
pobj |
stimulation,to |
R9118 |
T10329 |
T10315 |
punct |
.,washed |