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PMC:3342329 / 33686-34891 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T16467 0-4 NNP denotes Flow
T16468 5-15 NNP denotes Cytometric
T16469 16-24 NNP denotes Analysis
T16470 25-36 NNPS denotes Lymphocytes
T16471 37-41 VBD denotes were
T16472 42-50 VBN denotes obtained
T16473 51-55 IN denotes from
T16474 56-61 NN denotes tumor
T16475 62-65 CC denotes and
T16476 66-72 NN denotes spleen
T16477 73-80 NNS denotes tissues
T16478 81-84 IN denotes for
T16479 85-94 NN denotes phenotype
T16480 95-103 NN denotes analysis
T16481 103-104 . denotes .
T16482 105-111 NNP denotes Spleen
T16483 112-115 CC denotes and
T16484 116-121 NN denotes tumor
T16485 122-133 NNS denotes lymphocytes
T16486 134-138 VBD denotes were
T16487 139-147 VBN denotes isolated
T16488 148-152 IN denotes from
T16489 153-158 JJ denotes fresh
T16490 159-165 NN denotes spleen
T16491 166-169 CC denotes and
T16492 170-175 NN denotes tumor
T16493 176-184 NNS denotes biopsies
T16494 185-193 VBN denotes obtained
T16495 194-198 IN denotes from
T16496 199-204 CD denotes three
T16497 205-209 NNS denotes mice
T16498 210-213 IN denotes per
T16499 214-219 NN denotes group
T16500 219-220 . denotes .
T16501 221-227 NNP denotes Spleen
T16502 228-231 CC denotes and
T16503 232-237 NN denotes tumor
T16504 238-246 NNS denotes biopsies
T16505 247-251 VBD denotes were
T16506 252-259 RB denotes briefly
T16507 260-271 VBN denotes homogenized
T16508 272-284 RB denotes mechanically
T16509 285-287 IN denotes in
T16510 288-291 NNP denotes PBS
T16511 291-292 , denotes ,
T16512 293-301 VBN denotes filtered
T16513 302-303 -LRB- denotes (
T16514 303-306 CD denotes 100
T16515 307-309 NN denotes µm
T16516 310-314 NN denotes cell
T16517 315-323 NN denotes strainer
T16518 323-324 : denotes ;
T16519 325-327 NNP denotes BD
T16520 328-338 NNP denotes PharMingen
T16521 338-339 -RRB- denotes )
T16522 340-343 CC denotes and
T16523 344-348 RB denotes then
T16524 349-355 VBN denotes placed
T16525 356-358 IN denotes in
T16526 359-364 NN denotes lysis
T16527 365-371 NN denotes buffer
T16528 372-373 -LRB- denotes (
T16529 373-376 NNP denotes ACK
T16530 377-383 VBG denotes lysing
T16531 384-390 NN denotes buffer
T16532 390-391 , denotes ,
T16533 392-397 NNP denotes LONZA
T16534 397-398 -RRB- denotes )
T16535 399-401 TO denotes to
T16536 402-408 VB denotes remove
T16537 409-412 JJ denotes red
T16538 413-418 NN denotes blood
T16539 419-424 NNS denotes cells
T16540 424-425 . denotes .
T16541 426-429 CD denotes One
T16542 430-437 CD denotes million
T16543 438-449 NNS denotes splenocytes
T16544 450-454 VBD denotes were
T16545 455-461 VBN denotes washed
T16546 462-466 RB denotes once
T16547 467-469 IN denotes in
T16548 470-473 NNP denotes PBS
T16549 474-484 VBG denotes containing
T16550 485-486 CD denotes 1
T16551 486-487 NN denotes %
T16552 488-494 JJ denotes bovine
T16553 495-500 NN denotes serum
T16554 501-508 NN denotes albumin
T16555 509-510 -LRB- denotes (
T16556 510-513 NNP denotes BSA
T16557 513-514 -RRB- denotes )
T16558 515-518 CC denotes and
T16559 519-531 JJ denotes re-suspended
T16560 532-534 IN denotes in
T16561 535-538 CD denotes 100
T16562 539-541 NN denotes µl
T16563 542-544 IN denotes of
T16564 545-552 NNP denotes PBS/BSA
T16565 553-559 NN denotes buffer
T16566 559-560 . denotes .
T16567 561-570 RB denotes Meanwhile
T16568 570-571 , denotes ,
T16569 572-575 CD denotes one
T16570 576-583 CD denotes million
T16571 584-589 NNS denotes cells
T16572 590-598 VBN denotes obtained
T16573 599-603 IN denotes from
T16574 604-609 NN denotes tumor
T16575 610-617 NNS denotes tissues
T16576 618-622 VBD denotes were
T16577 623-629 VBN denotes placed
T16578 630-632 IN denotes in
T16579 633-637 NNP denotes RPMI
T16580 638-644 NN denotes medium
T16581 644-645 , denotes ,
T16582 646-655 VBN denotes incubated
T16583 656-658 TO denotes to
T16584 659-665 VB denotes obtain
T16585 666-676 NN denotes suspension
T16586 677-681 NN denotes cell
T16587 682-685 IN denotes for
T16588 686-691 CD denotes 1hour
T16589 692-694 IN denotes at
T16590 695-697 CD denotes 37
T16591 697-698 CD denotes °
T16592 698-699 NNP denotes C
T16593 699-700 , denotes ,
T16594 701-712 VBN denotes centrifuged
T16595 713-714 -LRB- denotes (
T16596 714-718 CD denotes 1200
T16597 719-722 NN denotes rpm
T16598 722-723 , denotes ,
T16599 724-725 CD denotes 3
T16600 726-729 NN denotes min
T16601 729-730 -RRB- denotes )
T16602 731-734 CC denotes and
T16603 735-739 RB denotes then
T16604 740-746 VBD denotes washed
T16605 747-749 IN denotes in
T16606 750-753 NNP denotes PBS
T16607 753-754 . denotes .
T16608 755-759 JJ denotes Next
T16609 759-760 , denotes ,
T16610 761-764 DT denotes the
T16611 765-776 NNS denotes lymphocytes
T16612 777-781 VBD denotes were
T16613 782-791 VBN denotes incubated
T16614 792-796 IN denotes with
T16615 797-804 JJ denotes various
T16616 805-815 VBN denotes conjugated
T16617 816-826 JJ denotes monoclonal
T16618 827-837 NNS denotes antibodies
T16619 837-838 , denotes ,
T16620 839-848 VBG denotes including
T16621 849-856 NNP denotes CD4-APC
T16622 856-857 , denotes ,
T16623 858-866 NNP denotes CD8-FITC
T16624 867-870 CC denotes and
T16625 871-878 NNP denotes CD19-PE
T16626 879-882 IN denotes for
T16627 883-888 CD denotes 20min
T16628 889-891 IN denotes at
T16629 892-893 CD denotes 4
T16630 893-894 CD denotes °
T16631 894-895 NNP denotes C
T16632 895-896 , denotes ,
T16633 897-903 VBD denotes washed
T16634 904-909 RB denotes twice
T16635 910-912 IN denotes in
T16636 913-916 NNP denotes PBS
T16637 916-917 , denotes ,
T16638 918-921 CC denotes and
T16639 922-934 JJ denotes re-suspended
T16640 935-937 IN denotes in
T16641 938-941 CD denotes 400
T16642 942-944 NN denotes µl
T16643 945-947 IN denotes of
T16644 948-951 NNP denotes PBS
T16645 951-952 . denotes .
T16646 953-954 DT denotes A
T16647 955-959 NN denotes flow
T16648 960-970 JJ denotes cytometric
T16649 971-979 NN denotes analysis
T16650 980-983 VBD denotes was
T16651 984-993 VBN denotes performed
T16652 994-996 IN denotes on
T16653 997-998 DT denotes a
T16654 999-1007 NNP denotes FACSAria
T16655 1008-1012 NN denotes flow
T16656 1013-1022 NN denotes cytometry
T16657 1023-1024 -LRB- denotes (
T16658 1024-1026 NNP denotes BD
T16659 1027-1038 NNPS denotes Biosciences
T16660 1038-1039 -RRB- denotes )
T16661 1039-1040 , denotes ,
T16662 1041-1044 CC denotes and
T16663 1045-1048 DT denotes the
T16664 1049-1053 NNS denotes data
T16665 1054-1058 VBD denotes were
T16666 1059-1067 VBN denotes analyzed
T16667 1068-1073 VBG denotes using
T16668 1074-1077 DT denotes the
T16669 1078-1084 NNP denotes WinMDI
T16670 1085-1096 JJ denotes statistical
T16671 1097-1105 NN denotes software
T16672 1106-1107 -LRB- denotes (
T16673 1107-1114 NNP denotes Scripps
T16674 1114-1115 , denotes ,
T16675 1116-1118 NNP denotes La
T16676 1119-1124 NNP denotes Jolla
T16677 1124-1125 , denotes ,
T16678 1126-1128 NNP denotes CA
T16679 1128-1129 , denotes ,
T16680 1130-1133 NNP denotes USA
T16681 1133-1134 -RRB- denotes )
T16682 1134-1135 . denotes .
T16683 1136-1143 RB denotes Forward
T16684 1144-1147 CC denotes and
T16685 1148-1152 NN denotes side
T16686 1153-1160 NN denotes scatter
T16687 1161-1171 NNS denotes parameters
T16688 1172-1176 VBD denotes were
T16689 1177-1181 VBN denotes used
T16690 1182-1184 TO denotes to
T16691 1185-1189 NN denotes gate
T16692 1190-1192 IN denotes on
T16693 1193-1204 NNS denotes lymphocytes
T16694 1204-1205 . denotes .
R11813 T16467 T16470 compound Flow,Lymphocytes
R11814 T16468 T16470 compound Cytometric,Lymphocytes
R11815 T16469 T16470 compound Analysis,Lymphocytes
R11816 T16470 T16472 nsubjpass Lymphocytes,obtained
R11817 T16471 T16472 auxpass were,obtained
R11818 T16472 T16472 ROOT obtained,obtained
R11819 T16473 T16472 prep from,obtained
R11820 T16474 T16477 nmod tumor,tissues
R11821 T16475 T16474 cc and,tumor
R11822 T16476 T16474 conj spleen,tumor
R11823 T16477 T16473 pobj tissues,from
R11824 T16478 T16477 prep for,tissues
R11825 T16479 T16480 compound phenotype,analysis
R11826 T16480 T16478 pobj analysis,for
R11827 T16481 T16472 punct .,obtained
R11828 T16482 T16486 nsubj Spleen,were
R11829 T16483 T16482 cc and,Spleen
R11830 T16484 T16485 compound tumor,lymphocytes
R11831 T16485 T16482 conj lymphocytes,Spleen
R11832 T16486 T16487 auxpass were,isolated
R11833 T16487 T16487 ROOT isolated,isolated
R11834 T16488 T16487 prep from,isolated
R11835 T16489 T16490 amod fresh,spleen
R11836 T16490 T16488 pobj spleen,from
R11837 T16491 T16490 cc and,spleen
R11838 T16492 T16493 compound tumor,biopsies
R11839 T16493 T16490 conj biopsies,spleen
R11840 T16494 T16487 conj obtained,isolated
R11841 T16495 T16494 prep from,obtained
R11842 T16496 T16497 nummod three,mice
R11843 T16497 T16495 pobj mice,from
R11844 T16498 T16497 prep per,mice
R11845 T16499 T16498 pobj group,per
R11846 T16500 T16487 punct .,isolated
R11847 T16501 T16507 nsubjpass Spleen,homogenized
R11848 T16502 T16501 cc and,Spleen
R11849 T16503 T16504 compound tumor,biopsies
R11850 T16504 T16501 conj biopsies,Spleen
R11851 T16505 T16507 auxpass were,homogenized
R11852 T16506 T16507 advmod briefly,homogenized
R11853 T16507 T16524 ccomp homogenized,placed
R11854 T16508 T16507 advmod mechanically,homogenized
R11855 T16509 T16507 prep in,homogenized
R11856 T16510 T16509 pobj PBS,in
R11857 T16511 T16507 punct ",",homogenized
R11858 T16512 T16507 conj filtered,homogenized
R11859 T16513 T16517 punct (,strainer
R11860 T16514 T16517 nummod 100,strainer
R11861 T16515 T16517 compound µm,strainer
R11862 T16516 T16517 compound cell,strainer
R11863 T16517 T16512 appos strainer,filtered
R11864 T16518 T16524 punct ;,placed
R11865 T16519 T16520 compound BD,PharMingen
R11866 T16520 T16524 nsubj PharMingen,placed
R11867 T16521 T16520 punct ),PharMingen
R11868 T16522 T16520 cc and,PharMingen
R11869 T16523 T16524 advmod then,placed
R11870 T16524 T16524 ROOT placed,placed
R11871 T16525 T16524 prep in,placed
R11872 T16526 T16527 compound lysis,buffer
R11873 T16527 T16525 pobj buffer,in
R11874 T16528 T16531 punct (,buffer
R11875 T16529 T16531 compound ACK,buffer
R11876 T16530 T16531 compound lysing,buffer
R11877 T16531 T16527 appos buffer,buffer
R11878 T16532 T16531 punct ",",buffer
R11879 T16533 T16531 appos LONZA,buffer
R11880 T16534 T16531 punct ),buffer
R11881 T16535 T16536 aux to,remove
R11882 T16536 T16524 advcl remove,placed
R11883 T16537 T16539 amod red,cells
R11884 T16538 T16539 compound blood,cells
R11885 T16539 T16536 dobj cells,remove
R11886 T16540 T16524 punct .,placed
R11887 T16541 T16542 compound One,million
R11888 T16542 T16543 nummod million,splenocytes
R11889 T16543 T16545 nsubjpass splenocytes,washed
R11890 T16544 T16545 auxpass were,washed
R11891 T16545 T16545 ROOT washed,washed
R11892 T16546 T16545 advmod once,washed
R11893 T16547 T16545 prep in,washed
R11894 T16548 T16547 pobj PBS,in
R11895 T16549 T16545 advcl containing,washed
R11896 T16550 T16551 nummod 1,%
R11897 T16551 T16554 compound %,albumin
R11898 T16552 T16553 amod bovine,serum
R11899 T16553 T16554 compound serum,albumin
R11900 T16554 T16549 dobj albumin,containing
R11901 T16555 T16556 punct (,BSA
R11902 T16556 T16554 appos BSA,albumin
R11903 T16557 T16556 punct ),BSA
R11904 T16558 T16554 cc and,albumin
R11905 T16559 T16554 conj re-suspended,albumin
R11906 T16560 T16559 prep in,re-suspended
R11907 T16561 T16562 nummod 100,µl
R11908 T16562 T16560 pobj µl,in
R11909 T16563 T16562 prep of,µl
R11910 T16564 T16565 compound PBS/BSA,buffer
R11911 T16565 T16563 pobj buffer,of
R11912 T16566 T16545 punct .,washed
R11913 T16567 T16577 advmod Meanwhile,placed
R11914 T16568 T16577 punct ",",placed
R11915 T16569 T16570 compound one,million
R11916 T16570 T16571 nummod million,cells
R11917 T16571 T16577 nsubjpass cells,placed
R11918 T16572 T16571 acl obtained,cells
R11919 T16573 T16572 prep from,obtained
R11920 T16574 T16575 compound tumor,tissues
R11921 T16575 T16573 pobj tissues,from
R11922 T16576 T16577 auxpass were,placed
R11923 T16577 T16577 ROOT placed,placed
R11924 T16578 T16577 prep in,placed
R11925 T16579 T16580 compound RPMI,medium
R11926 T16580 T16578 pobj medium,in
R11927 T16581 T16577 punct ",",placed
R11928 T16582 T16577 conj incubated,placed
R11929 T16583 T16584 aux to,obtain
R11930 T16584 T16582 advcl obtain,incubated
R11931 T16585 T16586 compound suspension,cell
R11932 T16586 T16584 dobj cell,obtain
R11933 T16587 T16586 prep for,cell
R11934 T16588 T16587 pobj 1hour,for
R11935 T16589 T16584 prep at,obtain
R11936 T16590 T16591 nummod 37,°
R11937 T16591 T16592 nummod °,C
R11938 T16592 T16589 pobj C,at
R11939 T16593 T16592 punct ",",C
R11940 T16594 T16592 amod centrifuged,C
R11941 T16595 T16594 punct (,centrifuged
R11942 T16596 T16597 nummod 1200,rpm
R11943 T16597 T16594 appos rpm,centrifuged
R11944 T16598 T16600 punct ",",min
R11945 T16599 T16600 nummod 3,min
R11946 T16600 T16597 appos min,rpm
R11947 T16601 T16597 punct ),rpm
R11948 T16602 T16594 cc and,centrifuged
R11949 T16603 T16604 advmod then,washed
R11950 T16604 T16594 conj washed,centrifuged
R11951 T16605 T16604 prep in,washed
R11952 T16606 T16605 pobj PBS,in
R11953 T16607 T16577 punct .,placed
R11954 T16608 T16613 advmod Next,incubated
R11955 T16609 T16613 punct ",",incubated
R11956 T16610 T16611 det the,lymphocytes
R11957 T16611 T16613 nsubjpass lymphocytes,incubated
R11958 T16612 T16613 auxpass were,incubated
R11959 T16613 T16613 ROOT incubated,incubated
R11960 T16614 T16613 prep with,incubated
R11961 T16615 T16618 amod various,antibodies
R11962 T16616 T16618 amod conjugated,antibodies
R11963 T16617 T16618 amod monoclonal,antibodies
R11964 T16618 T16614 pobj antibodies,with
R11965 T16619 T16618 punct ",",antibodies
R11966 T16620 T16618 prep including,antibodies
R11967 T16621 T16620 pobj CD4-APC,including
R11968 T16622 T16621 punct ",",CD4-APC
R11969 T16623 T16621 conj CD8-FITC,CD4-APC
R11970 T16624 T16623 cc and,CD8-FITC
R11971 T16625 T16623 conj CD19-PE,CD8-FITC
R11972 T16626 T16613 prep for,incubated
R11973 T16627 T16626 pobj 20min,for
R11974 T16628 T16613 prep at,incubated
R11975 T16629 T16630 nummod 4,°
R11976 T16630 T16631 nummod °,C
R11977 T16631 T16628 pobj C,at
R11978 T16632 T16633 punct ",",washed
R11979 T16633 T16613 dep washed,incubated
R11980 T16634 T16633 advmod twice,washed
R11981 T16635 T16633 prep in,washed
R11982 T16636 T16635 pobj PBS,in
R11983 T16637 T16633 punct ",",washed
R11984 T16638 T16633 cc and,washed
R11985 T16639 T16633 conj re-suspended,washed
R11986 T16640 T16639 prep in,re-suspended
R11987 T16641 T16642 nummod 400,µl
R11988 T16642 T16640 pobj µl,in
R11989 T16643 T16642 prep of,µl
R11990 T16644 T16643 pobj PBS,of
R11991 T16645 T16613 punct .,incubated
R11992 T16646 T16649 det A,analysis
R11993 T16647 T16649 nmod flow,analysis
R11994 T16648 T16649 amod cytometric,analysis
R11995 T16649 T16651 nsubjpass analysis,performed
R11996 T16650 T16651 auxpass was,performed
R11997 T16651 T16651 ROOT performed,performed
R11998 T16652 T16651 prep on,performed
R11999 T16653 T16655 det a,flow
R12000 T16654 T16655 compound FACSAria,flow
R12001 T16655 T16652 pobj flow,on
R12002 T16656 T16651 conj cytometry,performed
R12003 T16657 T16659 punct (,Biosciences
R12004 T16658 T16659 compound BD,Biosciences
R12005 T16659 T16656 appos Biosciences,cytometry
R12006 T16660 T16659 punct ),Biosciences
R12007 T16661 T16656 punct ",",cytometry
R12008 T16662 T16656 cc and,cytometry
R12009 T16663 T16664 det the,data
R12010 T16664 T16666 nsubjpass data,analyzed
R12011 T16665 T16666 auxpass were,analyzed
R12012 T16666 T16656 conj analyzed,cytometry
R12013 T16667 T16666 advcl using,analyzed
R12014 T16668 T16671 det the,software
R12015 T16669 T16671 nmod WinMDI,software
R12016 T16670 T16671 amod statistical,software
R12017 T16671 T16667 dobj software,using
R12018 T16672 T16681 punct (,)
R12019 T16673 T16681 nmod Scripps,)
R12020 T16674 T16673 punct ",",Scripps
R12021 T16675 T16676 compound La,Jolla
R12022 T16676 T16673 conj Jolla,Scripps
R12023 T16677 T16676 punct ",",Jolla
R12024 T16678 T16676 conj CA,Jolla
R12025 T16679 T16678 punct ",",CA
R12026 T16680 T16678 conj USA,CA
R12027 T16681 T16671 punct ),software
R12028 T16682 T16666 punct .,analyzed
R12029 T16683 T16687 amod Forward,parameters
R12030 T16684 T16683 cc and,Forward
R12031 T16685 T16687 compound side,parameters
R12032 T16686 T16687 compound scatter,parameters
R12033 T16687 T16689 nsubjpass parameters,used
R12034 T16688 T16689 auxpass were,used
R12035 T16689 T16689 ROOT used,used
R12036 T16690 T16691 aux to,gate
R12037 T16691 T16689 xcomp gate,used
R12038 T16692 T16691 prep on,gate
R12039 T16693 T16692 pobj lymphocytes,on
R12040 T16694 T16689 punct .,used