| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3697 |
0-3 |
CD |
denotes |
2.6 |
| T3698 |
3-4 |
. |
denotes |
. |
| T3699 |
5-12 |
JJ |
denotes |
Western |
| T3700 |
13-17 |
NNP |
denotes |
Blot |
| T3701 |
18-20 |
TO |
denotes |
To |
| T3702 |
21-27 |
VB |
denotes |
obtain |
| T3703 |
28-33 |
JJ |
denotes |
total |
| T3704 |
34-41 |
NN |
denotes |
protein |
| T3705 |
42-49 |
VBZ |
denotes |
lysates |
| T3706 |
49-50 |
, |
denotes |
, |
| T3707 |
51-56 |
NNS |
denotes |
cells |
| T3708 |
57-61 |
VBD |
denotes |
were |
| T3709 |
62-73 |
VBN |
denotes |
homogenized |
| T3710 |
74-76 |
IN |
denotes |
in |
| T3711 |
77-81 |
NNP |
denotes |
RIPA |
| T3712 |
82-88 |
NN |
denotes |
buffer |
| T3713 |
89-90 |
-LRB- |
denotes |
( |
| T3714 |
90-91 |
CD |
denotes |
1 |
| T3715 |
91-92 |
NN |
denotes |
% |
| T3716 |
93-97 |
CD |
denotes |
NP40 |
| T3717 |
97-98 |
, |
denotes |
, |
| T3718 |
99-102 |
CD |
denotes |
0.5 |
| T3719 |
102-103 |
NN |
denotes |
% |
| T3720 |
104-110 |
NN |
denotes |
sodium |
| T3721 |
111-123 |
NN |
denotes |
deoxycholate |
| T3722 |
123-124 |
, |
denotes |
, |
| T3723 |
125-128 |
CD |
denotes |
0.1 |
| T3724 |
128-129 |
NN |
denotes |
% |
| T3725 |
130-133 |
NNP |
denotes |
SDS |
| T3726 |
133-134 |
, |
denotes |
, |
| T3727 |
135-136 |
CD |
denotes |
1 |
| T3728 |
137-139 |
NNP |
denotes |
mM |
| T3729 |
140-144 |
NNP |
denotes |
EDTA |
| T3730 |
144-145 |
, |
denotes |
, |
| T3731 |
146-147 |
CD |
denotes |
1 |
| T3732 |
148-150 |
NNP |
denotes |
mM |
| T3733 |
151-155 |
NNP |
denotes |
EGTA |
| T3734 |
155-156 |
, |
denotes |
, |
| T3735 |
157-158 |
CD |
denotes |
1 |
| T3736 |
159-161 |
NNP |
denotes |
mM |
| T3737 |
162-168 |
NNP |
denotes |
Na3VO4 |
| T3738 |
168-169 |
, |
denotes |
, |
| T3739 |
170-172 |
CD |
denotes |
20 |
| T3740 |
173-175 |
NNP |
denotes |
mM |
| T3741 |
176-179 |
NNP |
denotes |
NaF |
| T3742 |
179-180 |
, |
denotes |
, |
| T3743 |
181-184 |
CD |
denotes |
0.5 |
| T3744 |
185-187 |
NNP |
denotes |
mM |
| T3745 |
188-191 |
NNP |
denotes |
DTT |
| T3746 |
191-192 |
, |
denotes |
, |
| T3747 |
193-194 |
CD |
denotes |
1 |
| T3748 |
195-197 |
NNP |
denotes |
mM |
| T3749 |
198-202 |
NNP |
denotes |
PMSF |
| T3750 |
202-203 |
, |
denotes |
, |
| T3751 |
204-207 |
CC |
denotes |
and |
| T3752 |
208-216 |
NN |
denotes |
protease |
| T3753 |
217-226 |
NN |
denotes |
inhibitor |
| T3754 |
227-235 |
NN |
denotes |
cocktail |
| T3755 |
236-238 |
IN |
denotes |
in |
| T3756 |
239-242 |
NNP |
denotes |
PBS |
| T3757 |
243-245 |
NNP |
denotes |
pH |
| T3758 |
246-249 |
CD |
denotes |
7.4 |
| T3759 |
249-250 |
-RRB- |
denotes |
) |
| T3760 |
251-254 |
CC |
denotes |
and |
| T3761 |
255-266 |
VBN |
denotes |
centrifuged |
| T3762 |
267-269 |
IN |
denotes |
at |
| T3763 |
270-276 |
CD |
denotes |
12,000 |
| T3764 |
277-278 |
NN |
denotes |
g |
| T3765 |
279-282 |
IN |
denotes |
for |
| T3766 |
283-285 |
CD |
denotes |
15 |
| T3767 |
286-289 |
NN |
denotes |
min |
| T3768 |
290-292 |
IN |
denotes |
at |
| T3769 |
293-294 |
CD |
denotes |
4 |
| T3770 |
294-295 |
CD |
denotes |
° |
| T3771 |
295-297 |
NNP |
denotes |
C. |
| T3772 |
298-305 |
NNP |
denotes |
Protein |
| T3773 |
306-320 |
NNS |
denotes |
concentrations |
| T3774 |
321-325 |
VBD |
denotes |
were |
| T3775 |
326-335 |
VBN |
denotes |
estimated |
| T3776 |
336-338 |
IN |
denotes |
by |
| T3777 |
339-348 |
NNP |
denotes |
Coomassie |
| T3778 |
349-353 |
NNP |
denotes |
Plus |
| T3779 |
354-361 |
NNP |
denotes |
Protein |
| T3780 |
362-367 |
NNP |
denotes |
Assay |
| T3781 |
368-375 |
NNP |
denotes |
Reagent |
| T3782 |
376-377 |
-LRB- |
denotes |
( |
| T3783 |
377-383 |
NNP |
denotes |
Pierce |
| T3784 |
383-384 |
, |
denotes |
, |
| T3785 |
385-387 |
NNP |
denotes |
IL |
| T3786 |
387-388 |
, |
denotes |
, |
| T3787 |
389-392 |
NNP |
denotes |
USA |
| T3788 |
392-393 |
-RRB- |
denotes |
) |
| T3789 |
393-394 |
. |
denotes |
. |
| T3790 |
395-400 |
NNP |
denotes |
Fifty |
| T3791 |
401-411 |
NNS |
denotes |
micrograms |
| T3792 |
412-414 |
IN |
denotes |
of |
| T3793 |
415-418 |
DT |
denotes |
the |
| T3794 |
419-428 |
VBG |
denotes |
resulting |
| T3795 |
429-438 |
JJ |
denotes |
cytosolic |
| T3796 |
439-446 |
NN |
denotes |
protein |
| T3797 |
447-455 |
NNS |
denotes |
extracts |
| T3798 |
456-460 |
VBD |
denotes |
were |
| T3799 |
461-475 |
JJ |
denotes |
heat-denatured |
| T3800 |
476-478 |
IN |
denotes |
in |
| T3801 |
479-486 |
NNP |
denotes |
Laemmli |
| T3802 |
487-493 |
NN |
denotes |
sample |
| T3803 |
494-501 |
VBG |
denotes |
loading |
| T3804 |
502-508 |
NN |
denotes |
buffer |
| T3805 |
508-509 |
, |
denotes |
, |
| T3806 |
510-519 |
VBN |
denotes |
separated |
| T3807 |
520-522 |
IN |
denotes |
by |
| T3808 |
523-525 |
CD |
denotes |
10 |
| T3809 |
525-526 |
NN |
denotes |
% |
| T3810 |
527-533 |
NN |
denotes |
sodium |
| T3811 |
534-541 |
NN |
denotes |
dodecyl |
| T3812 |
542-549 |
NN |
denotes |
sulfate |
| T3813 |
550-564 |
NN |
denotes |
polyacrylamide |
| T3814 |
565-568 |
NN |
denotes |
gel |
| T3815 |
569-584 |
NN |
denotes |
electrophoresis |
| T3816 |
584-585 |
, |
denotes |
, |
| T3817 |
586-589 |
CC |
denotes |
and |
| T3818 |
590-604 |
VBD |
denotes |
electroblotted |
| T3819 |
605-609 |
IN |
denotes |
onto |
| T3820 |
610-611 |
DT |
denotes |
a |
| T3821 |
612-626 |
JJ |
denotes |
nitrocellulose |
| T3822 |
627-635 |
NN |
denotes |
membrane |
| T3823 |
635-636 |
. |
denotes |
. |
| T3824 |
637-640 |
IN |
denotes |
For |
| T3825 |
641-655 |
VBG |
denotes |
immunoblotting |
| T3826 |
655-656 |
, |
denotes |
, |
| T3827 |
657-666 |
NNS |
denotes |
membranes |
| T3828 |
667-671 |
VBD |
denotes |
were |
| T3829 |
672-679 |
VBN |
denotes |
blocked |
| T3830 |
680-684 |
IN |
denotes |
with |
| T3831 |
685-686 |
CD |
denotes |
5 |
| T3832 |
686-687 |
NN |
denotes |
% |
| T3833 |
688-694 |
JJ |
denotes |
nonfat |
| T3834 |
695-698 |
JJ |
denotes |
dry |
| T3835 |
699-703 |
NN |
denotes |
milk |
| T3836 |
704-706 |
IN |
denotes |
in |
| T3837 |
707-713 |
NN |
denotes |
saline |
| T3838 |
714-720 |
NN |
denotes |
buffer |
| T3839 |
721-730 |
JJ |
denotes |
overnight |
| T3840 |
731-733 |
IN |
denotes |
at |
| T3841 |
734-735 |
CD |
denotes |
4 |
| T3842 |
735-736 |
CD |
denotes |
° |
| T3843 |
736-737 |
NNP |
denotes |
C |
| T3844 |
737-738 |
, |
denotes |
, |
| T3845 |
739-742 |
CC |
denotes |
and |
| T3846 |
743-746 |
DT |
denotes |
the |
| T3847 |
747-756 |
VBG |
denotes |
following |
| T3848 |
757-767 |
NNS |
denotes |
antibodies |
| T3849 |
768-772 |
VBD |
denotes |
were |
| T3850 |
773-777 |
VBN |
denotes |
used |
| T3851 |
777-778 |
: |
denotes |
: |
| T3852 |
779-791 |
JJ |
denotes |
anti-β-actin |
| T3853 |
792-793 |
-LRB- |
denotes |
( |
| T3854 |
793-802 |
JJ |
denotes |
sc-130657 |
| T3855 |
802-803 |
, |
denotes |
, |
| T3856 |
804-809 |
NNP |
denotes |
Santa |
| T3857 |
810-814 |
NNP |
denotes |
Cruz |
| T3858 |
815-828 |
NNP |
denotes |
Biotechnology |
| T3859 |
828-829 |
, |
denotes |
, |
| T3860 |
830-832 |
NNP |
denotes |
CA |
| T3861 |
832-833 |
, |
denotes |
, |
| T3862 |
834-837 |
NNP |
denotes |
USA |
| T3863 |
837-838 |
, |
denotes |
, |
| T3864 |
839-841 |
CD |
denotes |
43 |
| T3865 |
842-845 |
NN |
denotes |
kDa |
| T3866 |
845-846 |
-RRB- |
denotes |
) |
| T3867 |
847-850 |
CC |
denotes |
and |
| T3868 |
851-862 |
JJ |
denotes |
anti-matrix |
| T3869 |
863-879 |
NN |
denotes |
metallopeptidase |
| T3870 |
880-881 |
CD |
denotes |
9 |
| T3871 |
882-883 |
-LRB- |
denotes |
( |
| T3872 |
883-887 |
NNP |
denotes |
MMP9 |
| T3873 |
887-888 |
-RRB- |
denotes |
) |
| T3874 |
889-890 |
-LRB- |
denotes |
( |
| T3875 |
890-897 |
JJ |
denotes |
sc-6841 |
| T3876 |
897-898 |
, |
denotes |
, |
| T3877 |
899-904 |
NNP |
denotes |
Santa |
| T3878 |
905-909 |
NNP |
denotes |
Cruz |
| T3879 |
910-923 |
NNP |
denotes |
Biotechnology |
| T3880 |
923-924 |
, |
denotes |
, |
| T3881 |
925-927 |
NNP |
denotes |
CA |
| T3882 |
927-928 |
, |
denotes |
, |
| T3883 |
929-932 |
NNP |
denotes |
USA |
| T3884 |
932-933 |
, |
denotes |
, |
| T3885 |
934-936 |
CD |
denotes |
92 |
| T3886 |
937-940 |
NN |
denotes |
kDa |
| T3887 |
940-941 |
-RRB- |
denotes |
) |
| T3888 |
941-942 |
. |
denotes |
. |
| T3889 |
943-947 |
DT |
denotes |
Each |
| T3890 |
948-955 |
JJ |
denotes |
primary |
| T3891 |
956-964 |
NN |
denotes |
antibody |
| T3892 |
965-968 |
VBD |
denotes |
was |
| T3893 |
969-976 |
VBN |
denotes |
diluted |
| T3894 |
977-990 |
RB |
denotes |
appropriately |
| T3895 |
991-993 |
IN |
denotes |
in |
| T3896 |
994-1002 |
VBG |
denotes |
blocking |
| T3897 |
1003-1009 |
NN |
denotes |
buffer |
| T3898 |
1010-1013 |
CC |
denotes |
and |
| T3899 |
1014-1018 |
RB |
denotes |
then |
| T3900 |
1019-1024 |
VBD |
denotes |
added |
| T3901 |
1025-1027 |
TO |
denotes |
to |
| T3902 |
1028-1031 |
DT |
denotes |
the |
| T3903 |
1032-1037 |
NNS |
denotes |
blots |
| T3904 |
1038-1041 |
IN |
denotes |
for |
| T3905 |
1042-1043 |
CD |
denotes |
1 |
| T3906 |
1044-1045 |
NN |
denotes |
h |
| T3907 |
1046-1048 |
IN |
denotes |
at |
| T3908 |
1049-1053 |
NN |
denotes |
room |
| T3909 |
1054-1065 |
NN |
denotes |
temperature |
| T3910 |
1065-1066 |
. |
denotes |
. |
| T3911 |
1067-1070 |
DT |
denotes |
The |
| T3912 |
1071-1076 |
NNS |
denotes |
blots |
| T3913 |
1077-1081 |
VBD |
denotes |
were |
| T3914 |
1082-1088 |
VBN |
denotes |
washed |
| T3915 |
1089-1094 |
CD |
denotes |
three |
| T3916 |
1095-1100 |
NNS |
denotes |
times |
| T3917 |
1101-1103 |
IN |
denotes |
in |
| T3918 |
1104-1107 |
DT |
denotes |
the |
| T3919 |
1108-1115 |
VBG |
denotes |
washing |
| T3920 |
1116-1122 |
NN |
denotes |
buffer |
| T3921 |
1123-1126 |
CC |
denotes |
and |
| T3922 |
1127-1134 |
VBN |
denotes |
covered |
| T3923 |
1135-1139 |
IN |
denotes |
with |
| T3924 |
1140-1143 |
DT |
denotes |
the |
| T3925 |
1144-1155 |
JJ |
denotes |
horseradish |
| T3926 |
1156-1173 |
JJ |
denotes |
peroxidase-linked |
| T3927 |
1174-1183 |
JJ |
denotes |
secondary |
| T3928 |
1184-1192 |
NN |
denotes |
antibody |
| T3929 |
1193-1195 |
IN |
denotes |
at |
| T3930 |
1196-1197 |
DT |
denotes |
a |
| T3931 |
1198-1199 |
CD |
denotes |
1 |
| T3932 |
1200-1201 |
: |
denotes |
: |
| T3933 |
1202-1206 |
CD |
denotes |
2000 |
| T3934 |
1207-1215 |
NN |
denotes |
dilution |
| T3935 |
1216-1219 |
IN |
denotes |
for |
| T3936 |
1220-1221 |
CD |
denotes |
1 |
| T3937 |
1222-1224 |
NN |
denotes |
h. |
| T3938 |
1225-1230 |
NNS |
denotes |
Blots |
| T3939 |
1231-1235 |
VBD |
denotes |
were |
| T3940 |
1236-1245 |
VBN |
denotes |
incubated |
| T3941 |
1246-1250 |
IN |
denotes |
with |
| T3942 |
1251-1259 |
JJ |
denotes |
enhanced |
| T3943 |
1260-1277 |
NN |
denotes |
chemiluminescence |
| T3944 |
1278-1279 |
-LRB- |
denotes |
( |
| T3945 |
1279-1282 |
NNP |
denotes |
ECL |
| T3946 |
1282-1283 |
-RRB- |
denotes |
) |
| T3947 |
1284-1293 |
NN |
denotes |
detection |
| T3948 |
1294-1300 |
NN |
denotes |
system |
| T3949 |
1301-1302 |
-LRB- |
denotes |
( |
| T3950 |
1302-1310 |
NNP |
denotes |
Amersham |
| T3951 |
1311-1322 |
NNP |
denotes |
Biosciences |
| T3952 |
1322-1323 |
, |
denotes |
, |
| T3953 |
1324-1329 |
NNP |
denotes |
Bucks |
| T3954 |
1329-1330 |
, |
denotes |
, |
| T3955 |
1331-1333 |
NNP |
denotes |
UK |
| T3956 |
1333-1334 |
-RRB- |
denotes |
) |
| T3957 |
1335-1338 |
CC |
denotes |
and |
| T3958 |
1339-1346 |
VBN |
denotes |
exposed |
| T3959 |
1347-1349 |
TO |
denotes |
to |
| T3960 |
1350-1362 |
JJ |
denotes |
radiographic |
| T3961 |
1363-1367 |
NN |
denotes |
film |
| T3962 |
1368-1369 |
-LRB- |
denotes |
( |
| T3963 |
1369-1373 |
NNP |
denotes |
Fuji |
| T3964 |
1374-1383 |
NNP |
denotes |
Hyperfilm |
| T3965 |
1383-1384 |
, |
denotes |
, |
| T3966 |
1385-1390 |
NNP |
denotes |
Tokyo |
| T3967 |
1390-1391 |
, |
denotes |
, |
| T3968 |
1392-1397 |
NNP |
denotes |
Japan |
| T3969 |
1397-1398 |
-RRB- |
denotes |
) |
| T3970 |
1398-1399 |
. |
denotes |
. |
| T3971 |
1400-1406 |
NNP |
denotes |
ImageJ |
| T3972 |
1407-1410 |
VBD |
denotes |
was |
| T3973 |
1411-1415 |
VBN |
denotes |
used |
| T3974 |
1416-1418 |
TO |
denotes |
to |
| T3975 |
1419-1426 |
VB |
denotes |
analyze |
| T3976 |
1427-1430 |
DT |
denotes |
the |
| T3977 |
1431-1440 |
NN |
denotes |
intensity |
| T3978 |
1441-1443 |
IN |
denotes |
of |
| T3979 |
1444-1447 |
DT |
denotes |
the |
| T3980 |
1448-1453 |
NNS |
denotes |
blots |
| T3981 |
1453-1454 |
. |
denotes |
. |
| T3982 |
1455-1458 |
DT |
denotes |
The |
| T3983 |
1459-1464 |
NN |
denotes |
level |
| T3984 |
1465-1467 |
IN |
denotes |
of |
| T3985 |
1468-1475 |
NN |
denotes |
β-actin |
| T3986 |
1476-1479 |
VBD |
denotes |
was |
| T3987 |
1480-1484 |
VBN |
denotes |
used |
| T3988 |
1485-1487 |
IN |
denotes |
as |
| T3989 |
1488-1496 |
JJ |
denotes |
internal |
| T3990 |
1497-1505 |
NN |
denotes |
standard |
| T3991 |
1505-1506 |
. |
denotes |
. |
| R3191 |
T3697 |
T3697 |
ROOT |
2.6,2.6 |
| R3192 |
T3698 |
T3697 |
punct |
.,2.6 |
| R3193 |
T3699 |
T3700 |
compound |
Western,Blot |
| R3194 |
T3700 |
T3702 |
nsubj |
Blot,obtain |
| R3195 |
T3701 |
T3702 |
aux |
To,obtain |
| R3196 |
T3702 |
T3702 |
ROOT |
obtain,obtain |
| R3197 |
T3703 |
T3704 |
amod |
total,protein |
| R3198 |
T3704 |
T3702 |
dobj |
protein,obtain |
| R3199 |
T3705 |
T3709 |
advmod |
lysates,homogenized |
| R3200 |
T3706 |
T3709 |
punct |
",",homogenized |
| R3201 |
T3707 |
T3709 |
nsubjpass |
cells,homogenized |
| R3202 |
T3708 |
T3709 |
auxpass |
were,homogenized |
| R3203 |
T3709 |
T3702 |
advcl |
homogenized,obtain |
| R3204 |
T3710 |
T3709 |
prep |
in,homogenized |
| R3205 |
T3711 |
T3712 |
compound |
RIPA,buffer |
| R3206 |
T3712 |
T3710 |
pobj |
buffer,in |
| R3207 |
T3713 |
T3715 |
punct |
(,% |
| R3208 |
T3714 |
T3715 |
nummod |
1,% |
| R3209 |
T3715 |
T3775 |
nsubjpass |
%,estimated |
| R3210 |
T3716 |
T3721 |
nummod |
NP40,deoxycholate |
| R3211 |
T3717 |
T3716 |
punct |
",",NP40 |
| R3212 |
T3718 |
T3719 |
nummod |
0.5,% |
| R3213 |
T3719 |
T3721 |
nmod |
%,deoxycholate |
| R3214 |
T3720 |
T3721 |
compound |
sodium,deoxycholate |
| R3215 |
T3721 |
T3715 |
appos |
deoxycholate,% |
| R3216 |
T3722 |
T3721 |
punct |
",",deoxycholate |
| R3217 |
T3723 |
T3724 |
nummod |
0.1,% |
| R3218 |
T3724 |
T3715 |
appos |
%,% |
| R3219 |
T3725 |
T3715 |
npadvmod |
SDS,% |
| R3220 |
T3726 |
T3725 |
punct |
",",SDS |
| R3221 |
T3727 |
T3729 |
nummod |
1,EDTA |
| R3222 |
T3728 |
T3729 |
compound |
mM,EDTA |
| R3223 |
T3729 |
T3725 |
appos |
EDTA,SDS |
| R3224 |
T3730 |
T3725 |
punct |
",",SDS |
| R3225 |
T3731 |
T3733 |
nummod |
1,EGTA |
| R3226 |
T3732 |
T3733 |
compound |
mM,EGTA |
| R3227 |
T3733 |
T3725 |
appos |
EGTA,SDS |
| R3228 |
T3734 |
T3725 |
punct |
",",SDS |
| R3229 |
T3735 |
T3737 |
nummod |
1,Na3VO4 |
| R3230 |
T3736 |
T3737 |
compound |
mM,Na3VO4 |
| R3231 |
T3737 |
T3725 |
appos |
Na3VO4,SDS |
| R3232 |
T3738 |
T3725 |
punct |
",",SDS |
| R3233 |
T3739 |
T3741 |
nummod |
20,NaF |
| R3234 |
T3740 |
T3741 |
compound |
mM,NaF |
| R3235 |
T3741 |
T3725 |
appos |
NaF,SDS |
| R3236 |
T3742 |
T3725 |
punct |
",",SDS |
| R3237 |
T3743 |
T3745 |
nummod |
0.5,DTT |
| R3238 |
T3744 |
T3745 |
compound |
mM,DTT |
| R3239 |
T3745 |
T3725 |
appos |
DTT,SDS |
| R3240 |
T3746 |
T3725 |
punct |
",",SDS |
| R3241 |
T3747 |
T3749 |
nummod |
1,PMSF |
| R3242 |
T3748 |
T3749 |
compound |
mM,PMSF |
| R3243 |
T3749 |
T3725 |
appos |
PMSF,SDS |
| R3244 |
T3750 |
T3725 |
punct |
",",SDS |
| R3245 |
T3751 |
T3715 |
cc |
and,% |
| R3246 |
T3752 |
T3753 |
compound |
protease,inhibitor |
| R3247 |
T3753 |
T3754 |
compound |
inhibitor,cocktail |
| R3248 |
T3754 |
T3715 |
conj |
cocktail,% |
| R3249 |
T3755 |
T3715 |
prep |
in,% |
| R3250 |
T3756 |
T3755 |
pobj |
PBS,in |
| R3251 |
T3757 |
T3758 |
quantmod |
pH,7.4 |
| R3252 |
T3758 |
T3715 |
dep |
7.4,% |
| R3253 |
T3759 |
T3758 |
punct |
),7.4 |
| R3254 |
T3760 |
T3715 |
cc |
and,% |
| R3255 |
T3761 |
T3715 |
conj |
centrifuged,% |
| R3256 |
T3762 |
T3761 |
prep |
at,centrifuged |
| R3257 |
T3763 |
T3764 |
nummod |
"12,000",g |
| R3258 |
T3764 |
T3762 |
pobj |
g,at |
| R3259 |
T3765 |
T3761 |
prep |
for,centrifuged |
| R3260 |
T3766 |
T3767 |
nummod |
15,min |
| R3261 |
T3767 |
T3765 |
pobj |
min,for |
| R3262 |
T3768 |
T3767 |
prep |
at,min |
| R3263 |
T3769 |
T3770 |
nummod |
4,° |
| R3264 |
T3770 |
T3773 |
nummod |
°,concentrations |
| R3265 |
T3771 |
T3773 |
nmod |
C.,concentrations |
| R3266 |
T3772 |
T3773 |
compound |
Protein,concentrations |
| R3267 |
T3773 |
T3768 |
pobj |
concentrations,at |
| R3268 |
T3774 |
T3775 |
auxpass |
were,estimated |
| R3269 |
T3775 |
T3702 |
conj |
estimated,obtain |
| R3270 |
T3776 |
T3775 |
agent |
by,estimated |
| R3271 |
T3777 |
T3781 |
compound |
Coomassie,Reagent |
| R3272 |
T3778 |
T3779 |
compound |
Plus,Protein |
| R3273 |
T3779 |
T3781 |
compound |
Protein,Reagent |
| R3274 |
T3780 |
T3781 |
compound |
Assay,Reagent |
| R3275 |
T3781 |
T3776 |
pobj |
Reagent,by |
| R3276 |
T3782 |
T3783 |
punct |
(,Pierce |
| R3277 |
T3783 |
T3781 |
appos |
Pierce,Reagent |
| R3278 |
T3784 |
T3783 |
punct |
",",Pierce |
| R3279 |
T3785 |
T3783 |
prep |
IL,Pierce |
| R3280 |
T3786 |
T3785 |
punct |
",",IL |
| R3281 |
T3787 |
T3785 |
npadvmod |
USA,IL |
| R3282 |
T3788 |
T3783 |
punct |
),Pierce |
| R3283 |
T3789 |
T3775 |
punct |
.,estimated |
| R3284 |
T3790 |
T3791 |
nummod |
Fifty,micrograms |
| R3285 |
T3791 |
T3798 |
nsubj |
micrograms,were |
| R3286 |
T3792 |
T3791 |
prep |
of,micrograms |
| R3287 |
T3793 |
T3797 |
det |
the,extracts |
| R3288 |
T3794 |
T3797 |
amod |
resulting,extracts |
| R3289 |
T3795 |
T3797 |
amod |
cytosolic,extracts |
| R3290 |
T3796 |
T3797 |
compound |
protein,extracts |
| R3291 |
T3797 |
T3792 |
pobj |
extracts,of |
| R3292 |
T3798 |
T3798 |
ROOT |
were,were |
| R3293 |
T3799 |
T3798 |
acomp |
heat-denatured,were |
| R3294 |
T3800 |
T3799 |
prep |
in,heat-denatured |
| R3295 |
T3801 |
T3804 |
compound |
Laemmli,buffer |
| R3296 |
T3802 |
T3804 |
compound |
sample,buffer |
| R3297 |
T3803 |
T3804 |
compound |
loading,buffer |
| R3298 |
T3804 |
T3800 |
pobj |
buffer,in |
| R3299 |
T3805 |
T3799 |
punct |
",",heat-denatured |
| R3300 |
T3806 |
T3798 |
advcl |
separated,were |
| R3301 |
T3807 |
T3806 |
agent |
by,separated |
| R3302 |
T3808 |
T3809 |
nummod |
10,% |
| R3303 |
T3809 |
T3815 |
compound |
%,electrophoresis |
| R3304 |
T3810 |
T3812 |
compound |
sodium,sulfate |
| R3305 |
T3811 |
T3812 |
compound |
dodecyl,sulfate |
| R3306 |
T3812 |
T3814 |
compound |
sulfate,gel |
| R3307 |
T3813 |
T3814 |
compound |
polyacrylamide,gel |
| R3308 |
T3814 |
T3815 |
compound |
gel,electrophoresis |
| R3309 |
T3815 |
T3807 |
pobj |
electrophoresis,by |
| R3310 |
T3816 |
T3806 |
punct |
",",separated |
| R3311 |
T3817 |
T3806 |
cc |
and,separated |
| R3312 |
T3818 |
T3806 |
conj |
electroblotted,separated |
| R3313 |
T3819 |
T3818 |
prep |
onto,electroblotted |
| R3314 |
T3820 |
T3822 |
det |
a,membrane |
| R3315 |
T3821 |
T3822 |
amod |
nitrocellulose,membrane |
| R3316 |
T3822 |
T3819 |
pobj |
membrane,onto |
| R3317 |
T3823 |
T3798 |
punct |
.,were |
| R3318 |
T3824 |
T3829 |
prep |
For,blocked |
| R3319 |
T3825 |
T3824 |
pobj |
immunoblotting,For |
| R3320 |
T3826 |
T3829 |
punct |
",",blocked |
| R3321 |
T3827 |
T3829 |
nsubjpass |
membranes,blocked |
| R3322 |
T3828 |
T3829 |
auxpass |
were,blocked |
| R3323 |
T3829 |
T3829 |
ROOT |
blocked,blocked |
| R3324 |
T3830 |
T3829 |
prep |
with,blocked |
| R3325 |
T3831 |
T3832 |
nummod |
5,% |
| R3326 |
T3832 |
T3835 |
nmod |
%,milk |
| R3327 |
T3833 |
T3835 |
amod |
nonfat,milk |
| R3328 |
T3834 |
T3835 |
amod |
dry,milk |
| R3329 |
T3835 |
T3830 |
pobj |
milk,with |
| R3330 |
T3836 |
T3835 |
prep |
in,milk |
| R3331 |
T3837 |
T3838 |
compound |
saline,buffer |
| R3332 |
T3838 |
T3839 |
compound |
buffer,overnight |
| R3333 |
T3839 |
T3836 |
pobj |
overnight,in |
| R3334 |
T3840 |
T3829 |
prep |
at,blocked |
| R3335 |
T3841 |
T3842 |
nummod |
4,° |
| R3336 |
T3842 |
T3843 |
nummod |
°,C |
| R3337 |
T3843 |
T3840 |
pobj |
C,at |
| R3338 |
T3844 |
T3829 |
punct |
",",blocked |
| R3339 |
T3845 |
T3829 |
cc |
and,blocked |
| R3340 |
T3846 |
T3848 |
det |
the,antibodies |
| R3341 |
T3847 |
T3848 |
amod |
following,antibodies |
| R3342 |
T3848 |
T3850 |
nsubjpass |
antibodies,used |
| R3343 |
T3849 |
T3850 |
auxpass |
were,used |
| R3344 |
T3850 |
T3829 |
conj |
used,blocked |
| R3345 |
T3851 |
T3852 |
punct |
:,anti-β-actin |
| R3346 |
T3852 |
T3850 |
prep |
anti-β-actin,used |
| R3347 |
T3853 |
T3854 |
punct |
(,sc-130657 |
| R3348 |
T3854 |
T3852 |
dep |
sc-130657,anti-β-actin |
| R3349 |
T3855 |
T3854 |
punct |
",",sc-130657 |
| R3350 |
T3856 |
T3858 |
compound |
Santa,Biotechnology |
| R3351 |
T3857 |
T3858 |
compound |
Cruz,Biotechnology |
| R3352 |
T3858 |
T3854 |
conj |
Biotechnology,sc-130657 |
| R3353 |
T3859 |
T3858 |
punct |
",",Biotechnology |
| R3354 |
T3860 |
T3858 |
conj |
CA,Biotechnology |
| R3355 |
T3861 |
T3860 |
punct |
",",CA |
| R3356 |
T3862 |
T3860 |
conj |
USA,CA |
| R3357 |
T3863 |
T3860 |
punct |
",",CA |
| R3358 |
T3864 |
T3865 |
nummod |
43,kDa |
| R3359 |
T3865 |
T3860 |
appos |
kDa,CA |
| R3360 |
T3866 |
T3865 |
punct |
),kDa |
| R3361 |
T3867 |
T3865 |
cc |
and,kDa |
| R3362 |
T3868 |
T3869 |
amod |
anti-matrix,metallopeptidase |
| R3363 |
T3869 |
T3865 |
conj |
metallopeptidase,kDa |
| R3364 |
T3870 |
T3869 |
nummod |
9,metallopeptidase |
| R3365 |
T3871 |
T3872 |
punct |
(,MMP9 |
| R3366 |
T3872 |
T3869 |
appos |
MMP9,metallopeptidase |
| R3367 |
T3873 |
T3872 |
punct |
),MMP9 |
| R3368 |
T3874 |
T3875 |
punct |
(,sc-6841 |
| R3369 |
T3875 |
T3869 |
appos |
sc-6841,metallopeptidase |
| R3370 |
T3876 |
T3875 |
punct |
",",sc-6841 |
| R3371 |
T3877 |
T3879 |
compound |
Santa,Biotechnology |
| R3372 |
T3878 |
T3879 |
compound |
Cruz,Biotechnology |
| R3373 |
T3879 |
T3875 |
appos |
Biotechnology,sc-6841 |
| R3374 |
T3880 |
T3879 |
punct |
",",Biotechnology |
| R3375 |
T3881 |
T3879 |
conj |
CA,Biotechnology |
| R3376 |
T3882 |
T3881 |
punct |
",",CA |
| R3377 |
T3883 |
T3881 |
conj |
USA,CA |
| R3378 |
T3884 |
T3881 |
punct |
",",CA |
| R3379 |
T3885 |
T3886 |
nummod |
92,kDa |
| R3380 |
T3886 |
T3881 |
appos |
kDa,CA |
| R3381 |
T3887 |
T3875 |
punct |
),sc-6841 |
| R3382 |
T3888 |
T3854 |
punct |
.,sc-130657 |
| R3383 |
T3889 |
T3891 |
det |
Each,antibody |
| R3384 |
T3890 |
T3891 |
amod |
primary,antibody |
| R3385 |
T3891 |
T3893 |
nsubjpass |
antibody,diluted |
| R3386 |
T3892 |
T3893 |
auxpass |
was,diluted |
| R3387 |
T3893 |
T3893 |
ROOT |
diluted,diluted |
| R3388 |
T3894 |
T3893 |
advmod |
appropriately,diluted |
| R3389 |
T3895 |
T3893 |
prep |
in,diluted |
| R3390 |
T3896 |
T3895 |
pcomp |
blocking,in |
| R3391 |
T3897 |
T3896 |
dobj |
buffer,blocking |
| R3392 |
T3898 |
T3893 |
cc |
and,diluted |
| R3393 |
T3899 |
T3900 |
advmod |
then,added |
| R3394 |
T3900 |
T3893 |
conj |
added,diluted |
| R3395 |
T3901 |
T3900 |
prep |
to,added |
| R3396 |
T3902 |
T3903 |
det |
the,blots |
| R3397 |
T3903 |
T3901 |
pobj |
blots,to |
| R3398 |
T3904 |
T3900 |
prep |
for,added |
| R3399 |
T3905 |
T3906 |
nummod |
1,h |
| R3400 |
T3906 |
T3904 |
pobj |
h,for |
| R3401 |
T3907 |
T3906 |
prep |
at,h |
| R3402 |
T3908 |
T3909 |
compound |
room,temperature |
| R3403 |
T3909 |
T3907 |
pobj |
temperature,at |
| R3404 |
T3910 |
T3900 |
punct |
.,added |
| R3405 |
T3911 |
T3912 |
det |
The,blots |
| R3406 |
T3912 |
T3914 |
nsubjpass |
blots,washed |
| R3407 |
T3913 |
T3914 |
auxpass |
were,washed |
| R3408 |
T3914 |
T3914 |
ROOT |
washed,washed |
| R3409 |
T3915 |
T3916 |
nummod |
three,times |
| R3410 |
T3916 |
T3914 |
npadvmod |
times,washed |
| R3411 |
T3917 |
T3914 |
prep |
in,washed |
| R3412 |
T3918 |
T3920 |
det |
the,buffer |
| R3413 |
T3919 |
T3920 |
amod |
washing,buffer |
| R3414 |
T3920 |
T3917 |
pobj |
buffer,in |
| R3415 |
T3921 |
T3914 |
cc |
and,washed |
| R3416 |
T3922 |
T3914 |
conj |
covered,washed |
| R3417 |
T3923 |
T3922 |
prep |
with,covered |
| R3418 |
T3924 |
T3928 |
det |
the,antibody |
| R3419 |
T3925 |
T3928 |
amod |
horseradish,antibody |
| R3420 |
T3926 |
T3928 |
amod |
peroxidase-linked,antibody |
| R3421 |
T3927 |
T3928 |
amod |
secondary,antibody |
| R3422 |
T3928 |
T3923 |
pobj |
antibody,with |
| R3423 |
T3929 |
T3922 |
prep |
at,covered |
| R3424 |
T3930 |
T3931 |
det |
a,1 |
| R3425 |
T3931 |
T3929 |
pobj |
1,at |
| R3426 |
T3932 |
T3934 |
punct |
:,dilution |
| R3427 |
T3933 |
T3934 |
nummod |
2000,dilution |
| R3428 |
T3934 |
T3940 |
nsubjpass |
dilution,incubated |
| R3429 |
T3935 |
T3934 |
prep |
for,dilution |
| R3430 |
T3936 |
T3938 |
nummod |
1,Blots |
| R3431 |
T3937 |
T3938 |
compound |
h.,Blots |
| R3432 |
T3938 |
T3935 |
pobj |
Blots,for |
| R3433 |
T3939 |
T3940 |
auxpass |
were,incubated |
| R3434 |
T3940 |
T3914 |
conj |
incubated,washed |
| R3435 |
T3941 |
T3940 |
prep |
with,incubated |
| R3436 |
T3942 |
T3943 |
amod |
enhanced,chemiluminescence |
| R3437 |
T3943 |
T3941 |
pobj |
chemiluminescence,with |
| R3438 |
T3944 |
T3945 |
punct |
(,ECL |
| R3439 |
T3945 |
T3943 |
appos |
ECL,chemiluminescence |
| R3440 |
T3946 |
T3945 |
punct |
),ECL |
| R3441 |
T3947 |
T3948 |
compound |
detection,system |
| R3442 |
T3948 |
T3943 |
conj |
system,chemiluminescence |
| R3443 |
T3949 |
T3948 |
punct |
(,system |
| R3444 |
T3950 |
T3951 |
compound |
Amersham,Biosciences |
| R3445 |
T3951 |
T3948 |
appos |
Biosciences,system |
| R3446 |
T3952 |
T3951 |
punct |
",",Biosciences |
| R3447 |
T3953 |
T3951 |
conj |
Bucks,Biosciences |
| R3448 |
T3954 |
T3953 |
punct |
",",Bucks |
| R3449 |
T3955 |
T3953 |
conj |
UK,Bucks |
| R3450 |
T3956 |
T3953 |
punct |
),Bucks |
| R3451 |
T3957 |
T3940 |
cc |
and,incubated |
| R3452 |
T3958 |
T3940 |
conj |
exposed,incubated |
| R3453 |
T3959 |
T3958 |
prep |
to,exposed |
| R3454 |
T3960 |
T3961 |
amod |
radiographic,film |
| R3455 |
T3961 |
T3959 |
pobj |
film,to |
| R3456 |
T3962 |
T3964 |
punct |
(,Hyperfilm |
| R3457 |
T3963 |
T3964 |
compound |
Fuji,Hyperfilm |
| R3458 |
T3964 |
T3961 |
appos |
Hyperfilm,film |
| R3459 |
T3965 |
T3964 |
punct |
",",Hyperfilm |
| R3460 |
T3966 |
T3964 |
conj |
Tokyo,Hyperfilm |
| R3461 |
T3967 |
T3966 |
punct |
",",Tokyo |
| R3462 |
T3968 |
T3966 |
conj |
Japan,Tokyo |
| R3463 |
T3969 |
T3964 |
punct |
),Hyperfilm |
| R3464 |
T3970 |
T3940 |
punct |
.,incubated |
| R3465 |
T3971 |
T3973 |
nsubjpass |
ImageJ,used |
| R3466 |
T3972 |
T3973 |
auxpass |
was,used |
| R3467 |
T3973 |
T3973 |
ROOT |
used,used |
| R3468 |
T3974 |
T3975 |
aux |
to,analyze |
| R3469 |
T3975 |
T3973 |
xcomp |
analyze,used |
| R3470 |
T3976 |
T3977 |
det |
the,intensity |
| R3471 |
T3977 |
T3975 |
dobj |
intensity,analyze |
| R3472 |
T3978 |
T3977 |
prep |
of,intensity |
| R3473 |
T3979 |
T3980 |
det |
the,blots |
| R3474 |
T3980 |
T3978 |
pobj |
blots,of |
| R3475 |
T3981 |
T3973 |
punct |
.,used |
| R3476 |
T3982 |
T3983 |
det |
The,level |
| R3477 |
T3983 |
T3987 |
nsubjpass |
level,used |
| R3478 |
T3984 |
T3983 |
prep |
of,level |
| R3479 |
T3985 |
T3984 |
pobj |
β-actin,of |
| R3480 |
T3986 |
T3987 |
auxpass |
was,used |
| R3481 |
T3987 |
T3987 |
ROOT |
used,used |
| R3482 |
T3988 |
T3987 |
prep |
as,used |
| R3483 |
T3989 |
T3990 |
amod |
internal,standard |
| R3484 |
T3990 |
T3988 |
pobj |
standard,as |
| R3485 |
T3991 |
T3987 |
punct |
.,used |
