Id |
Subject |
Object |
Predicate |
Lexical cue |
T2683 |
0-10 |
NNPS |
denotes |
Monolayers |
T2684 |
11-13 |
IN |
denotes |
of |
T2685 |
14-24 |
NNS |
denotes |
astrocytes |
T2686 |
25-29 |
VBD |
denotes |
were |
T2687 |
30-36 |
VBN |
denotes |
washed |
T2688 |
37-41 |
IN |
denotes |
with |
T2689 |
42-45 |
NNP |
denotes |
PBS |
T2690 |
46-49 |
CC |
denotes |
and |
T2691 |
50-59 |
VBN |
denotes |
harvested |
T2692 |
60-62 |
IN |
denotes |
by |
T2693 |
63-71 |
VBG |
denotes |
scraping |
T2694 |
72-76 |
IN |
denotes |
into |
T2695 |
77-81 |
JJ |
denotes |
cold |
T2696 |
82-85 |
NNP |
denotes |
PBS |
T2697 |
85-86 |
. |
denotes |
. |
T2698 |
87-90 |
DT |
denotes |
The |
T2699 |
91-95 |
NN |
denotes |
cell |
T2700 |
96-102 |
NN |
denotes |
pellet |
T2701 |
103-111 |
VBN |
denotes |
obtained |
T2702 |
112-114 |
IN |
denotes |
by |
T2703 |
115-129 |
NN |
denotes |
centrifugation |
T2704 |
130-133 |
VBD |
denotes |
was |
T2705 |
134-145 |
VBN |
denotes |
resuspended |
T2706 |
146-148 |
IN |
denotes |
in |
T2707 |
149-155 |
NN |
denotes |
buffer |
T2708 |
156-166 |
VBG |
denotes |
containing |
T2709 |
167-169 |
CD |
denotes |
10 |
T2710 |
170-172 |
NNP |
denotes |
mM |
T2711 |
173-178 |
NNP |
denotes |
HEPES |
T2712 |
179-180 |
-LRB- |
denotes |
( |
T2713 |
180-182 |
NNP |
denotes |
pH |
T2714 |
183-186 |
CD |
denotes |
7.9 |
T2715 |
186-187 |
-RRB- |
denotes |
) |
T2716 |
187-188 |
, |
denotes |
, |
T2717 |
189-191 |
CD |
denotes |
10 |
T2718 |
192-194 |
NNP |
denotes |
mM |
T2719 |
195-198 |
NNP |
denotes |
KCl |
T2720 |
198-199 |
, |
denotes |
, |
T2721 |
200-203 |
CD |
denotes |
0.1 |
T2722 |
204-206 |
NNP |
denotes |
mM |
T2723 |
207-211 |
NNP |
denotes |
EDTA |
T2724 |
211-212 |
, |
denotes |
, |
T2725 |
213-216 |
CD |
denotes |
0.1 |
T2726 |
217-219 |
NNP |
denotes |
mM |
T2727 |
220-224 |
NNP |
denotes |
EGTA |
T2728 |
224-225 |
, |
denotes |
, |
T2729 |
226-229 |
CD |
denotes |
1.0 |
T2730 |
230-232 |
NNP |
denotes |
mM |
T2731 |
233-236 |
NNP |
denotes |
DTT |
T2732 |
236-237 |
, |
denotes |
, |
T2733 |
238-241 |
CC |
denotes |
and |
T2734 |
242-245 |
CD |
denotes |
0.5 |
T2735 |
246-248 |
NNP |
denotes |
mM |
T2736 |
249-269 |
NN |
denotes |
phenylmethylsulfonyl |
T2737 |
270-278 |
NN |
denotes |
fluoride |
T2738 |
278-279 |
. |
denotes |
. |
T2739 |
280-284 |
RB |
denotes |
Then |
T2740 |
285-287 |
CD |
denotes |
10 |
T2741 |
287-288 |
NN |
denotes |
% |
T2742 |
289-296 |
NNP |
denotes |
Nonidet |
T2743 |
297-301 |
NNP |
denotes |
P-40 |
T2744 |
302-305 |
VBD |
denotes |
was |
T2745 |
306-311 |
VBN |
denotes |
added |
T2746 |
312-315 |
CC |
denotes |
and |
T2747 |
316-324 |
VBN |
denotes |
vortexed |
T2748 |
325-332 |
NN |
denotes |
briefly |
T2749 |
332-333 |
, |
denotes |
, |
T2750 |
334-337 |
CC |
denotes |
and |
T2751 |
338-341 |
DT |
denotes |
the |
T2752 |
342-348 |
NNS |
denotes |
nuclei |
T2753 |
349-353 |
VBD |
denotes |
were |
T2754 |
354-362 |
VBN |
denotes |
pelleted |
T2755 |
363-365 |
IN |
denotes |
by |
T2756 |
366-380 |
NN |
denotes |
centrifugation |
T2757 |
380-381 |
. |
denotes |
. |
T2758 |
382-385 |
DT |
denotes |
The |
T2759 |
386-393 |
JJ |
denotes |
nuclear |
T2760 |
394-402 |
NNS |
denotes |
proteins |
T2761 |
403-407 |
VBD |
denotes |
were |
T2762 |
408-417 |
VBN |
denotes |
extracted |
T2763 |
418-422 |
IN |
denotes |
with |
T2764 |
423-429 |
NN |
denotes |
buffer |
T2765 |
430-440 |
VBG |
denotes |
containing |
T2766 |
441-443 |
CD |
denotes |
20 |
T2767 |
444-446 |
NNP |
denotes |
mM |
T2768 |
447-452 |
NNP |
denotes |
HEPES |
T2769 |
453-454 |
-LRB- |
denotes |
( |
T2770 |
454-456 |
NNP |
denotes |
pH |
T2771 |
457-460 |
CD |
denotes |
7.9 |
T2772 |
460-461 |
-RRB- |
denotes |
) |
T2773 |
461-462 |
, |
denotes |
, |
T2774 |
463-466 |
CD |
denotes |
0.4 |
T2775 |
467-469 |
NNP |
denotes |
mM |
T2776 |
470-474 |
NNP |
denotes |
NaCl |
T2777 |
474-475 |
, |
denotes |
, |
T2778 |
476-479 |
CD |
denotes |
1.0 |
T2779 |
480-482 |
NNP |
denotes |
mM |
T2780 |
483-487 |
NNP |
denotes |
EDTA |
T2781 |
487-488 |
, |
denotes |
, |
T2782 |
489-492 |
CD |
denotes |
1.0 |
T2783 |
493-495 |
NNP |
denotes |
mM |
T2784 |
496-500 |
NNP |
denotes |
EGTA |
T2785 |
500-501 |
, |
denotes |
, |
T2786 |
502-505 |
CD |
denotes |
1.0 |
T2787 |
506-508 |
NNP |
denotes |
mM |
T2788 |
509-512 |
NNP |
denotes |
DTT |
T2789 |
512-513 |
, |
denotes |
, |
T2790 |
514-517 |
CC |
denotes |
and |
T2791 |
518-521 |
CD |
denotes |
1.0 |
T2792 |
522-524 |
NNP |
denotes |
mM |
T2793 |
525-545 |
NN |
denotes |
phenylmethylsulfonyl |
T2794 |
546-554 |
NN |
denotes |
fluoride |
T2795 |
554-555 |
. |
denotes |
. |
T2796 |
556-565 |
NNP |
denotes |
Insoluble |
T2797 |
566-574 |
NN |
denotes |
material |
T2798 |
575-578 |
VBD |
denotes |
was |
T2799 |
579-586 |
VBN |
denotes |
removed |
T2800 |
587-589 |
IN |
denotes |
by |
T2801 |
590-604 |
NN |
denotes |
centrifugation |
T2802 |
605-607 |
IN |
denotes |
at |
T2803 |
608-613 |
CD |
denotes |
14000 |
T2804 |
614-617 |
NN |
denotes |
rpm |
T2805 |
617-618 |
, |
denotes |
, |
T2806 |
619-622 |
CC |
denotes |
and |
T2807 |
623-626 |
DT |
denotes |
the |
T2808 |
627-638 |
JJ |
denotes |
supernatant |
T2809 |
639-649 |
VBG |
denotes |
containing |
T2810 |
650-653 |
DT |
denotes |
the |
T2811 |
654-661 |
JJ |
denotes |
nuclear |
T2812 |
662-670 |
NNS |
denotes |
proteins |
T2813 |
671-674 |
VBD |
denotes |
was |
T2814 |
675-681 |
VBN |
denotes |
stored |
T2815 |
682-684 |
IN |
denotes |
at |
T2816 |
686-688 |
CD |
denotes |
80 |
T2817 |
688-689 |
CD |
denotes |
° |
T2818 |
689-690 |
NNP |
denotes |
C |
T2819 |
691-696 |
IN |
denotes |
until |
T2820 |
697-700 |
NN |
denotes |
use |
T2821 |
700-701 |
. |
denotes |
. |
T2822 |
702-709 |
NN |
denotes |
Protein |
T2823 |
710-723 |
NN |
denotes |
concentration |
T2824 |
724-727 |
VBD |
denotes |
was |
T2825 |
728-738 |
VBN |
denotes |
determined |
T2826 |
739-744 |
VBG |
denotes |
using |
T2827 |
745-746 |
DT |
denotes |
a |
T2828 |
747-760 |
JJ |
denotes |
bicinchoninic |
T2829 |
761-765 |
NN |
denotes |
acid |
T2830 |
766-771 |
NN |
denotes |
assay |
T2831 |
772-775 |
NN |
denotes |
kit |
T2832 |
776-780 |
IN |
denotes |
with |
T2833 |
781-787 |
JJ |
denotes |
bovine |
T2834 |
788-793 |
NN |
denotes |
serum |
T2835 |
794-801 |
NN |
denotes |
albumin |
T2836 |
802-804 |
IN |
denotes |
as |
T2837 |
805-808 |
DT |
denotes |
the |
T2838 |
809-817 |
NN |
denotes |
standard |
T2839 |
818-819 |
-LRB- |
denotes |
( |
T2840 |
819-825 |
NNP |
denotes |
Pierce |
T2841 |
826-838 |
NNP |
denotes |
Biochemicals |
T2842 |
838-839 |
, |
denotes |
, |
T2843 |
840-848 |
NNP |
denotes |
Rockford |
T2844 |
848-849 |
, |
denotes |
, |
T2845 |
850-852 |
NNP |
denotes |
IL |
T2846 |
852-853 |
, |
denotes |
, |
T2847 |
854-857 |
NNP |
denotes |
USA |
T2848 |
857-858 |
-RRB- |
denotes |
) |
T2849 |
858-859 |
. |
denotes |
. |
T2850 |
860-864 |
NNP |
denotes |
EMSA |
T2851 |
865-868 |
VBD |
denotes |
was |
T2852 |
869-878 |
VBN |
denotes |
performed |
T2853 |
879-884 |
VBG |
denotes |
using |
T2854 |
885-888 |
NN |
denotes |
gel |
T2855 |
889-894 |
NN |
denotes |
shift |
T2856 |
895-900 |
NN |
denotes |
assay |
T2857 |
901-907 |
NN |
denotes |
system |
T2858 |
908-909 |
-LRB- |
denotes |
( |
T2859 |
909-916 |
NNP |
denotes |
Promega |
T2860 |
916-917 |
, |
denotes |
, |
T2861 |
918-925 |
NNP |
denotes |
Madison |
T2862 |
925-926 |
, |
denotes |
, |
T2863 |
927-929 |
NNP |
denotes |
WI |
T2864 |
929-930 |
, |
denotes |
, |
T2865 |
931-934 |
NNP |
denotes |
USA |
T2866 |
934-935 |
-RRB- |
denotes |
) |
T2867 |
935-936 |
. |
denotes |
. |
T2868 |
937-946 |
NNP |
denotes |
Consensus |
T2869 |
947-962 |
NN |
denotes |
oligonucleotide |
T2870 |
963-968 |
NN |
denotes |
probe |
T2871 |
969-970 |
-LRB- |
denotes |
( |
T2872 |
970-971 |
CD |
denotes |
5 |
T2873 |
971-972 |
SYM |
denotes |
′ |
T2874 |
972-973 |
: |
denotes |
- |
T2875 |
973-997 |
NN |
denotes |
AGTTGAGGGGACTTTCCCAGGG-3 |
T2876 |
997-998 |
NN |
denotes |
′ |
T2877 |
998-999 |
-RRB- |
denotes |
) |
T2878 |
1000-1003 |
VBD |
denotes |
was |
T2879 |
1004-1015 |
VBN |
denotes |
end-labeled |
T2880 |
1016-1018 |
IN |
denotes |
by |
T2881 |
1019-1036 |
NN |
denotes |
T4-polynucleotide |
T2882 |
1037-1043 |
NN |
denotes |
kinase |
T2883 |
1044-1049 |
VBG |
denotes |
using |
T2884 |
1050-1051 |
NNP |
denotes |
[ |
T2885 |
1051-1056 |
NNP |
denotes |
γ-32P |
T2886 |
1056-1057 |
NNP |
denotes |
] |
T2887 |
1057-1058 |
: |
denotes |
- |
T2888 |
1058-1061 |
NNP |
denotes |
ATP |
T2889 |
1062-1063 |
-LRB- |
denotes |
( |
T2890 |
1063-1067 |
NNP |
denotes |
Free |
T2891 |
1068-1076 |
NNP |
denotes |
Biotech. |
T2892 |
1076-1077 |
, |
denotes |
, |
T2893 |
1078-1085 |
NNP |
denotes |
Beijing |
T2894 |
1085-1086 |
, |
denotes |
, |
T2895 |
1087-1092 |
NNP |
denotes |
China |
T2896 |
1092-1093 |
-RRB- |
denotes |
) |
T2897 |
1093-1094 |
. |
denotes |
. |
T2898 |
1095-1102 |
NNP |
denotes |
Nuclear |
T2899 |
1103-1110 |
NN |
denotes |
protein |
T2900 |
1111-1112 |
-LRB- |
denotes |
( |
T2901 |
1112-1114 |
CD |
denotes |
20 |
T2902 |
1115-1117 |
NN |
denotes |
μg |
T2903 |
1117-1118 |
-RRB- |
denotes |
) |
T2904 |
1119-1122 |
VBD |
denotes |
was |
T2905 |
1123-1135 |
VBN |
denotes |
preincubated |
T2906 |
1136-1138 |
IN |
denotes |
in |
T2907 |
1139-1141 |
CD |
denotes |
20 |
T2908 |
1142-1144 |
NN |
denotes |
μL |
T2909 |
1145-1152 |
JJ |
denotes |
binding |
T2910 |
1153-1159 |
NN |
denotes |
buffer |
T2911 |
1160-1170 |
VBG |
denotes |
containing |
T2912 |
1171-1173 |
CD |
denotes |
10 |
T2913 |
1174-1176 |
NNP |
denotes |
mM |
T2914 |
1177-1185 |
NNP |
denotes |
Tris-HCl |
T2915 |
1186-1187 |
-LRB- |
denotes |
( |
T2916 |
1187-1189 |
NNP |
denotes |
PH |
T2917 |
1190-1193 |
CD |
denotes |
7.5 |
T2918 |
1193-1194 |
-RRB- |
denotes |
) |
T2919 |
1194-1195 |
, |
denotes |
, |
T2920 |
1196-1197 |
CD |
denotes |
1 |
T2921 |
1198-1200 |
NNP |
denotes |
mM |
T2922 |
1201-1206 |
NNP |
denotes |
MgCl2 |
T2923 |
1206-1207 |
, |
denotes |
, |
T2924 |
1208-1211 |
CD |
denotes |
0.5 |
T2925 |
1212-1214 |
NNP |
denotes |
mM |
T2926 |
1215-1219 |
NNP |
denotes |
NaCl |
T2927 |
1219-1220 |
, |
denotes |
, |
T2928 |
1221-1222 |
CD |
denotes |
4 |
T2929 |
1222-1223 |
NN |
denotes |
% |
T2930 |
1224-1232 |
NN |
denotes |
glycerol |
T2931 |
1232-1233 |
, |
denotes |
, |
T2932 |
1234-1237 |
CD |
denotes |
0.5 |
T2933 |
1238-1240 |
NNP |
denotes |
mM |
T2934 |
1241-1245 |
NNP |
denotes |
EDTA |
T2935 |
1245-1246 |
, |
denotes |
, |
T2936 |
1247-1250 |
CD |
denotes |
0.5 |
T2937 |
1251-1253 |
NNP |
denotes |
mM |
T2938 |
1254-1257 |
NNP |
denotes |
DTT |
T2939 |
1257-1258 |
, |
denotes |
, |
T2940 |
1259-1262 |
CC |
denotes |
and |
T2941 |
1263-1264 |
CD |
denotes |
2 |
T2942 |
1265-1267 |
NN |
denotes |
μg |
T2943 |
1268-1272 |
NN |
denotes |
poly |
T2944 |
1273-1278 |
NN |
denotes |
dI-dC |
T2945 |
1279-1282 |
IN |
denotes |
for |
T2946 |
1283-1285 |
CD |
denotes |
20 |
T2947 |
1286-1293 |
NNS |
denotes |
minutes |
T2948 |
1294-1296 |
IN |
denotes |
on |
T2949 |
1297-1300 |
NN |
denotes |
ice |
T2950 |
1300-1301 |
. |
denotes |
. |
T2951 |
1302-1307 |
IN |
denotes |
After |
T2952 |
1308-1316 |
NN |
denotes |
addition |
T2953 |
1317-1319 |
IN |
denotes |
of |
T2954 |
1320-1323 |
DT |
denotes |
the |
T2955 |
1324-1325 |
CD |
denotes |
1 |
T2956 |
1326-1328 |
NNP |
denotes |
μL |
T2957 |
1329-1339 |
JJ |
denotes |
32P-labled |
T2958 |
1340-1355 |
NN |
denotes |
oligonucleotide |
T2959 |
1356-1361 |
NN |
denotes |
probe |
T2960 |
1361-1362 |
, |
denotes |
, |
T2961 |
1363-1366 |
DT |
denotes |
the |
T2962 |
1367-1377 |
NN |
denotes |
incubation |
T2963 |
1378-1381 |
VBD |
denotes |
was |
T2964 |
1382-1391 |
VBN |
denotes |
continued |
T2965 |
1392-1395 |
IN |
denotes |
for |
T2966 |
1396-1398 |
CD |
denotes |
20 |
T2967 |
1399-1406 |
NNS |
denotes |
minutes |
T2968 |
1407-1409 |
IN |
denotes |
on |
T2969 |
1410-1413 |
NN |
denotes |
ice |
T2970 |
1413-1414 |
. |
denotes |
. |
T2971 |
1415-1418 |
DT |
denotes |
The |
T2972 |
1419-1430 |
JJ |
denotes |
DNA-protein |
T2973 |
1431-1440 |
NNS |
denotes |
complexes |
T2974 |
1441-1445 |
VBD |
denotes |
were |
T2975 |
1446-1455 |
VBN |
denotes |
separated |
T2976 |
1456-1458 |
IN |
denotes |
by |
T2977 |
1459-1474 |
NN |
denotes |
electrophoresis |
T2978 |
1475-1477 |
IN |
denotes |
on |
T2979 |
1478-1479 |
CD |
denotes |
4 |
T2980 |
1479-1480 |
NN |
denotes |
% |
T2981 |
1481-1494 |
JJ |
denotes |
nondenaturing |
T2982 |
1495-1509 |
NN |
denotes |
polyacrylamide |
T2983 |
1510-1513 |
NN |
denotes |
gel |
T2984 |
1514-1516 |
IN |
denotes |
in |
T2985 |
1517-1520 |
CD |
denotes |
0.5 |
T2986 |
1521-1522 |
CD |
denotes |
× |
T2987 |
1523-1526 |
NNP |
denotes |
TBE |
T2988 |
1527-1533 |
NN |
denotes |
buffer |
T2989 |
1534-1535 |
-LRB- |
denotes |
( |
T2990 |
1535-1551 |
NNP |
denotes |
tris-borate-EDTA |
T2991 |
1551-1552 |
-RRB- |
denotes |
) |
T2992 |
1553-1555 |
IN |
denotes |
at |
T2993 |
1556-1559 |
CD |
denotes |
390 |
T2994 |
1560-1561 |
NN |
denotes |
V |
T2995 |
1562-1565 |
IN |
denotes |
for |
T2996 |
1566-1567 |
CD |
denotes |
1 |
T2997 |
1568-1572 |
NN |
denotes |
hour |
T2998 |
1573-1575 |
IN |
denotes |
at |
T2999 |
1576-1577 |
CD |
denotes |
4 |
T3000 |
1577-1578 |
CD |
denotes |
° |
T3001 |
1578-1579 |
NNP |
denotes |
C |
T3002 |
1579-1580 |
. |
denotes |
. |
T3003 |
1581-1586 |
IN |
denotes |
After |
T3004 |
1587-1602 |
NN |
denotes |
electrophoresis |
T3005 |
1602-1603 |
, |
denotes |
, |
T3006 |
1604-1607 |
DT |
denotes |
the |
T3007 |
1608-1611 |
NN |
denotes |
gel |
T3008 |
1612-1615 |
VBD |
denotes |
was |
T3009 |
1616-1621 |
VBN |
denotes |
dried |
T3010 |
1622-1625 |
CC |
denotes |
and |
T3011 |
1626-1633 |
VBN |
denotes |
exposed |
T3012 |
1634-1636 |
TO |
denotes |
to |
T3013 |
1637-1642 |
NN |
denotes |
X-ray |
T3014 |
1643-1647 |
NN |
denotes |
film |
T3015 |
1648-1649 |
-LRB- |
denotes |
( |
T3016 |
1649-1653 |
NNP |
denotes |
Fuji |
T3017 |
1654-1663 |
NNP |
denotes |
Hyperfilm |
T3018 |
1663-1664 |
, |
denotes |
, |
T3019 |
1665-1670 |
NNP |
denotes |
Tokyo |
T3020 |
1670-1671 |
, |
denotes |
, |
T3021 |
1672-1677 |
NNP |
denotes |
Japan |
T3022 |
1677-1678 |
-RRB- |
denotes |
) |
T3023 |
1678-1679 |
. |
denotes |
. |
T3024 |
1680-1686 |
NNS |
denotes |
Levels |
T3025 |
1687-1689 |
IN |
denotes |
of |
T3026 |
1690-1695 |
NNP |
denotes |
NF-κB |
T3027 |
1696-1699 |
NNP |
denotes |
DNA |
T3028 |
1700-1707 |
JJ |
denotes |
binding |
T3029 |
1708-1716 |
NN |
denotes |
activity |
T3030 |
1717-1721 |
VBD |
denotes |
were |
T3031 |
1722-1732 |
VBN |
denotes |
quantified |
T3032 |
1733-1735 |
IN |
denotes |
by |
T3033 |
1736-1744 |
NN |
denotes |
software |
T3034 |
1745-1751 |
NNP |
denotes |
ImageJ |
T3035 |
1751-1752 |
. |
denotes |
. |
R2323 |
T2684 |
T2683 |
prep |
of,Monolayers |
R2324 |
T2685 |
T2684 |
pobj |
astrocytes,of |
R2325 |
T2686 |
T2687 |
auxpass |
were,washed |
R2326 |
T2687 |
T2687 |
ROOT |
washed,washed |
R2327 |
T2688 |
T2687 |
prep |
with,washed |
R2328 |
T2689 |
T2688 |
pobj |
PBS,with |
R2329 |
T2690 |
T2687 |
cc |
and,washed |
R2330 |
T2691 |
T2687 |
conj |
harvested,washed |
R2331 |
T2692 |
T2691 |
prep |
by,harvested |
R2332 |
T2693 |
T2692 |
pcomp |
scraping,by |
R2333 |
T2694 |
T2693 |
prep |
into,scraping |
R2334 |
T2695 |
T2696 |
amod |
cold,PBS |
R2335 |
T2696 |
T2694 |
pobj |
PBS,into |
R2336 |
T2697 |
T2687 |
punct |
.,washed |
R2337 |
T2698 |
T2700 |
det |
The,pellet |
R2338 |
T2699 |
T2700 |
compound |
cell,pellet |
R2339 |
T2700 |
T2704 |
nsubj |
pellet,was |
R2340 |
T2701 |
T2700 |
acl |
obtained,pellet |
R2341 |
T2702 |
T2701 |
agent |
by,obtained |
R2342 |
T2703 |
T2702 |
pobj |
centrifugation,by |
R2343 |
T2704 |
T2705 |
auxpass |
was,resuspended |
R2344 |
T2705 |
T2705 |
ROOT |
resuspended,resuspended |
R2345 |
T2706 |
T2705 |
prep |
in,resuspended |
R2346 |
T2707 |
T2706 |
pobj |
buffer,in |
R2347 |
T2708 |
T2707 |
acl |
containing,buffer |
R2348 |
T2709 |
T2711 |
nummod |
10,HEPES |
R2349 |
T2710 |
T2711 |
compound |
mM,HEPES |
R2350 |
T2711 |
T2708 |
dobj |
HEPES,containing |
R2351 |
T2712 |
T2713 |
punct |
(,pH |
R2352 |
T2713 |
T2711 |
appos |
pH,HEPES |
R2353 |
T2714 |
T2713 |
nummod |
7.9,pH |
R2354 |
T2715 |
T2713 |
punct |
),pH |
R2355 |
T2716 |
T2711 |
punct |
",",HEPES |
R2356 |
T2717 |
T2719 |
nummod |
10,KCl |
R2357 |
T2718 |
T2719 |
compound |
mM,KCl |
R2358 |
T2719 |
T2711 |
conj |
KCl,HEPES |
R2359 |
T2720 |
T2719 |
punct |
",",KCl |
R2360 |
T2721 |
T2723 |
compound |
0.1,EDTA |
R2361 |
T2722 |
T2723 |
compound |
mM,EDTA |
R2362 |
T2723 |
T2719 |
conj |
EDTA,KCl |
R2363 |
T2724 |
T2723 |
punct |
",",EDTA |
R2364 |
T2725 |
T2727 |
nummod |
0.1,EGTA |
R2365 |
T2726 |
T2727 |
compound |
mM,EGTA |
R2366 |
T2727 |
T2723 |
conj |
EGTA,EDTA |
R2367 |
T2728 |
T2727 |
punct |
",",EGTA |
R2368 |
T2729 |
T2731 |
nummod |
1.0,DTT |
R2369 |
T2730 |
T2731 |
compound |
mM,DTT |
R2370 |
T2731 |
T2727 |
conj |
DTT,EGTA |
R2371 |
T2732 |
T2731 |
punct |
",",DTT |
R2372 |
T2733 |
T2731 |
cc |
and,DTT |
R2373 |
T2734 |
T2737 |
nummod |
0.5,fluoride |
R2374 |
T2735 |
T2737 |
nmod |
mM,fluoride |
R2375 |
T2736 |
T2737 |
compound |
phenylmethylsulfonyl,fluoride |
R2376 |
T2737 |
T2731 |
conj |
fluoride,DTT |
R2377 |
T2738 |
T2705 |
punct |
.,resuspended |
R2378 |
T2739 |
T2745 |
advmod |
Then,added |
R2379 |
T2740 |
T2741 |
nummod |
10,% |
R2380 |
T2741 |
T2743 |
compound |
%,P-40 |
R2381 |
T2742 |
T2743 |
compound |
Nonidet,P-40 |
R2382 |
T2743 |
T2745 |
nsubjpass |
P-40,added |
R2383 |
T2744 |
T2745 |
auxpass |
was,added |
R2384 |
T2745 |
T2745 |
ROOT |
added,added |
R2385 |
T2746 |
T2745 |
cc |
and,added |
R2386 |
T2747 |
T2745 |
conj |
vortexed,added |
R2387 |
T2748 |
T2747 |
advmod |
briefly,vortexed |
R2388 |
T2749 |
T2747 |
punct |
",",vortexed |
R2389 |
T2750 |
T2747 |
cc |
and,vortexed |
R2390 |
T2751 |
T2752 |
det |
the,nuclei |
R2391 |
T2752 |
T2754 |
nsubjpass |
nuclei,pelleted |
R2392 |
T2753 |
T2754 |
auxpass |
were,pelleted |
R2393 |
T2754 |
T2747 |
conj |
pelleted,vortexed |
R2394 |
T2755 |
T2754 |
agent |
by,pelleted |
R2395 |
T2756 |
T2755 |
pobj |
centrifugation,by |
R2396 |
T2757 |
T2754 |
punct |
.,pelleted |
R2397 |
T2758 |
T2760 |
det |
The,proteins |
R2398 |
T2759 |
T2760 |
amod |
nuclear,proteins |
R2399 |
T2760 |
T2762 |
nsubjpass |
proteins,extracted |
R2400 |
T2761 |
T2762 |
auxpass |
were,extracted |
R2401 |
T2762 |
T2762 |
ROOT |
extracted,extracted |
R2402 |
T2763 |
T2762 |
prep |
with,extracted |
R2403 |
T2764 |
T2763 |
pobj |
buffer,with |
R2404 |
T2765 |
T2764 |
acl |
containing,buffer |
R2405 |
T2766 |
T2768 |
nummod |
20,HEPES |
R2406 |
T2767 |
T2768 |
compound |
mM,HEPES |
R2407 |
T2768 |
T2765 |
dobj |
HEPES,containing |
R2408 |
T2769 |
T2770 |
punct |
(,pH |
R2409 |
T2770 |
T2768 |
appos |
pH,HEPES |
R2410 |
T2771 |
T2770 |
nummod |
7.9,pH |
R2411 |
T2772 |
T2770 |
punct |
),pH |
R2412 |
T2773 |
T2768 |
punct |
",",HEPES |
R2413 |
T2774 |
T2776 |
nummod |
0.4,NaCl |
R2414 |
T2775 |
T2776 |
compound |
mM,NaCl |
R2415 |
T2776 |
T2768 |
appos |
NaCl,HEPES |
R2416 |
T2777 |
T2776 |
punct |
",",NaCl |
R2417 |
T2778 |
T2780 |
nummod |
1.0,EDTA |
R2418 |
T2779 |
T2780 |
compound |
mM,EDTA |
R2419 |
T2780 |
T2776 |
conj |
EDTA,NaCl |
R2420 |
T2781 |
T2780 |
punct |
",",EDTA |
R2421 |
T2782 |
T2784 |
nummod |
1.0,EGTA |
R2422 |
T2783 |
T2784 |
compound |
mM,EGTA |
R2423 |
T2784 |
T2780 |
conj |
EGTA,EDTA |
R2424 |
T2785 |
T2784 |
punct |
",",EGTA |
R2425 |
T2786 |
T2788 |
nummod |
1.0,DTT |
R2426 |
T2787 |
T2788 |
compound |
mM,DTT |
R2427 |
T2788 |
T2784 |
conj |
DTT,EGTA |
R2428 |
T2789 |
T2788 |
punct |
",",DTT |
R2429 |
T2790 |
T2788 |
cc |
and,DTT |
R2430 |
T2791 |
T2794 |
nummod |
1.0,fluoride |
R2431 |
T2792 |
T2794 |
compound |
mM,fluoride |
R2432 |
T2793 |
T2794 |
compound |
phenylmethylsulfonyl,fluoride |
R2433 |
T2794 |
T2788 |
conj |
fluoride,DTT |
R2434 |
T2795 |
T2762 |
punct |
.,extracted |
R2435 |
T2796 |
T2797 |
amod |
Insoluble,material |
R2436 |
T2797 |
T2799 |
nsubjpass |
material,removed |
R2437 |
T2798 |
T2799 |
auxpass |
was,removed |
R2438 |
T2799 |
T2799 |
ROOT |
removed,removed |
R2439 |
T2800 |
T2799 |
agent |
by,removed |
R2440 |
T2801 |
T2800 |
pobj |
centrifugation,by |
R2441 |
T2802 |
T2799 |
prep |
at,removed |
R2442 |
T2803 |
T2804 |
nummod |
14000,rpm |
R2443 |
T2804 |
T2802 |
pobj |
rpm,at |
R2444 |
T2805 |
T2799 |
punct |
",",removed |
R2445 |
T2806 |
T2799 |
cc |
and,removed |
R2446 |
T2807 |
T2808 |
det |
the,supernatant |
R2447 |
T2808 |
T2814 |
nsubjpass |
supernatant,stored |
R2448 |
T2809 |
T2808 |
acl |
containing,supernatant |
R2449 |
T2810 |
T2812 |
det |
the,proteins |
R2450 |
T2811 |
T2812 |
amod |
nuclear,proteins |
R2451 |
T2812 |
T2809 |
dobj |
proteins,containing |
R2452 |
T2813 |
T2814 |
auxpass |
was,stored |
R2453 |
T2814 |
T2799 |
conj |
stored,removed |
R2454 |
T2815 |
T2814 |
prep |
at,stored |
R2455 |
T2816 |
T2818 |
nummod |
80,C |
R2456 |
T2817 |
T2818 |
nummod |
°,C |
R2457 |
T2818 |
T2815 |
pobj |
C,at |
R2458 |
T2819 |
T2814 |
prep |
until,stored |
R2459 |
T2820 |
T2819 |
pobj |
use,until |
R2460 |
T2821 |
T2814 |
punct |
.,stored |
R2461 |
T2822 |
T2823 |
compound |
Protein,concentration |
R2462 |
T2823 |
T2825 |
nsubjpass |
concentration,determined |
R2463 |
T2824 |
T2825 |
auxpass |
was,determined |
R2464 |
T2825 |
T2825 |
ROOT |
determined,determined |
R2465 |
T2826 |
T2825 |
advcl |
using,determined |
R2466 |
T2827 |
T2831 |
det |
a,kit |
R2467 |
T2828 |
T2831 |
amod |
bicinchoninic,kit |
R2468 |
T2829 |
T2830 |
compound |
acid,assay |
R2469 |
T2830 |
T2831 |
compound |
assay,kit |
R2470 |
T2831 |
T2826 |
dobj |
kit,using |
R2471 |
T2832 |
T2831 |
prep |
with,kit |
R2472 |
T2833 |
T2834 |
amod |
bovine,serum |
R2473 |
T2834 |
T2832 |
pobj |
serum,with |
R2474 |
T2835 |
T2831 |
amod |
albumin,kit |
R2475 |
T2836 |
T2826 |
prep |
as,using |
R2476 |
T2837 |
T2838 |
det |
the,standard |
R2477 |
T2838 |
T2836 |
pobj |
standard,as |
R2478 |
T2839 |
T2841 |
punct |
(,Biochemicals |
R2479 |
T2840 |
T2841 |
compound |
Pierce,Biochemicals |
R2480 |
T2841 |
T2826 |
parataxis |
Biochemicals,using |
R2481 |
T2842 |
T2841 |
punct |
",",Biochemicals |
R2482 |
T2843 |
T2841 |
npadvmod |
Rockford,Biochemicals |
R2483 |
T2844 |
T2843 |
punct |
",",Rockford |
R2484 |
T2845 |
T2843 |
conj |
IL,Rockford |
R2485 |
T2846 |
T2845 |
punct |
",",IL |
R2486 |
T2847 |
T2843 |
npadvmod |
USA,Rockford |
R2487 |
T2848 |
T2843 |
punct |
),Rockford |
R2488 |
T2849 |
T2841 |
punct |
.,Biochemicals |
R2489 |
T2850 |
T2852 |
nsubjpass |
EMSA,performed |
R2490 |
T2851 |
T2852 |
auxpass |
was,performed |
R2491 |
T2852 |
T2852 |
ROOT |
performed,performed |
R2492 |
T2853 |
T2852 |
advcl |
using,performed |
R2493 |
T2854 |
T2855 |
compound |
gel,shift |
R2494 |
T2855 |
T2857 |
compound |
shift,system |
R2495 |
T2856 |
T2857 |
compound |
assay,system |
R2496 |
T2857 |
T2853 |
dobj |
system,using |
R2497 |
T2858 |
T2857 |
punct |
(,system |
R2498 |
T2859 |
T2857 |
appos |
Promega,system |
R2499 |
T2860 |
T2859 |
punct |
",",Promega |
R2500 |
T2861 |
T2859 |
conj |
Madison,Promega |
R2501 |
T2862 |
T2861 |
punct |
",",Madison |
R2502 |
T2863 |
T2861 |
conj |
WI,Madison |
R2503 |
T2864 |
T2863 |
punct |
",",WI |
R2504 |
T2865 |
T2863 |
conj |
USA,WI |
R2505 |
T2866 |
T2857 |
punct |
),system |
R2506 |
T2867 |
T2852 |
punct |
.,performed |
R2507 |
T2868 |
T2870 |
compound |
Consensus,probe |
R2508 |
T2869 |
T2870 |
compound |
oligonucleotide,probe |
R2509 |
T2870 |
T2879 |
nsubjpass |
probe,end-labeled |
R2510 |
T2871 |
T2870 |
punct |
(,probe |
R2511 |
T2872 |
T2875 |
nummod |
5,AGTTGAGGGGACTTTCCCAGGG-3 |
R2512 |
T2873 |
T2875 |
punct |
′,AGTTGAGGGGACTTTCCCAGGG-3 |
R2513 |
T2874 |
T2875 |
punct |
-,AGTTGAGGGGACTTTCCCAGGG-3 |
R2514 |
T2875 |
T2870 |
appos |
AGTTGAGGGGACTTTCCCAGGG-3,probe |
R2515 |
T2876 |
T2875 |
appos |
′,AGTTGAGGGGACTTTCCCAGGG-3 |
R2516 |
T2877 |
T2875 |
punct |
),AGTTGAGGGGACTTTCCCAGGG-3 |
R2517 |
T2878 |
T2879 |
auxpass |
was,end-labeled |
R2518 |
T2879 |
T2879 |
ROOT |
end-labeled,end-labeled |
R2519 |
T2880 |
T2879 |
agent |
by,end-labeled |
R2520 |
T2881 |
T2882 |
compound |
T4-polynucleotide,kinase |
R2521 |
T2882 |
T2880 |
pobj |
kinase,by |
R2522 |
T2883 |
T2879 |
advcl |
using,end-labeled |
R2523 |
T2884 |
T2888 |
compound |
[,ATP |
R2524 |
T2885 |
T2886 |
compound |
γ-32P,] |
R2525 |
T2886 |
T2888 |
compound |
],ATP |
R2526 |
T2887 |
T2888 |
punct |
-,ATP |
R2527 |
T2888 |
T2883 |
dobj |
ATP,using |
R2528 |
T2889 |
T2891 |
punct |
(,Biotech. |
R2529 |
T2890 |
T2891 |
compound |
Free,Biotech. |
R2530 |
T2891 |
T2888 |
appos |
Biotech.,ATP |
R2531 |
T2892 |
T2891 |
punct |
",",Biotech. |
R2532 |
T2893 |
T2891 |
npadvmod |
Beijing,Biotech. |
R2533 |
T2894 |
T2893 |
punct |
",",Beijing |
R2534 |
T2895 |
T2891 |
npadvmod |
China,Biotech. |
R2535 |
T2896 |
T2891 |
punct |
),Biotech. |
R2536 |
T2897 |
T2879 |
punct |
.,end-labeled |
R2537 |
T2898 |
T2899 |
compound |
Nuclear,protein |
R2538 |
T2899 |
T2904 |
nsubj |
protein,was |
R2539 |
T2900 |
T2899 |
punct |
(,protein |
R2540 |
T2901 |
T2902 |
nummod |
20,μg |
R2541 |
T2902 |
T2899 |
appos |
μg,protein |
R2542 |
T2903 |
T2899 |
punct |
),protein |
R2543 |
T2904 |
T2905 |
auxpass |
was,preincubated |
R2544 |
T2905 |
T2905 |
ROOT |
preincubated,preincubated |
R2545 |
T2906 |
T2905 |
prep |
in,preincubated |
R2546 |
T2907 |
T2908 |
nummod |
20,μL |
R2547 |
T2908 |
T2910 |
nmod |
μL,buffer |
R2548 |
T2909 |
T2910 |
amod |
binding,buffer |
R2549 |
T2910 |
T2906 |
pobj |
buffer,in |
R2550 |
T2911 |
T2910 |
acl |
containing,buffer |
R2551 |
T2912 |
T2914 |
nummod |
10,Tris-HCl |
R2552 |
T2913 |
T2914 |
compound |
mM,Tris-HCl |
R2553 |
T2914 |
T2911 |
dobj |
Tris-HCl,containing |
R2554 |
T2915 |
T2916 |
punct |
(,PH |
R2555 |
T2916 |
T2914 |
appos |
PH,Tris-HCl |
R2556 |
T2917 |
T2916 |
nummod |
7.5,PH |
R2557 |
T2918 |
T2916 |
punct |
),PH |
R2558 |
T2919 |
T2914 |
punct |
",",Tris-HCl |
R2559 |
T2920 |
T2922 |
nummod |
1,MgCl2 |
R2560 |
T2921 |
T2922 |
compound |
mM,MgCl2 |
R2561 |
T2922 |
T2914 |
conj |
MgCl2,Tris-HCl |
R2562 |
T2923 |
T2922 |
punct |
",",MgCl2 |
R2563 |
T2924 |
T2922 |
npadvmod |
0.5,MgCl2 |
R2564 |
T2925 |
T2926 |
compound |
mM,NaCl |
R2565 |
T2926 |
T2926 |
ROOT |
NaCl,NaCl |
R2566 |
T2927 |
T2926 |
punct |
",",NaCl |
R2567 |
T2928 |
T2929 |
nummod |
4,% |
R2568 |
T2929 |
T2930 |
compound |
%,glycerol |
R2569 |
T2930 |
T2926 |
appos |
glycerol,NaCl |
R2570 |
T2931 |
T2930 |
punct |
",",glycerol |
R2571 |
T2932 |
T2934 |
nummod |
0.5,EDTA |
R2572 |
T2933 |
T2934 |
compound |
mM,EDTA |
R2573 |
T2934 |
T2926 |
conj |
EDTA,NaCl |
R2574 |
T2935 |
T2934 |
punct |
",",EDTA |
R2575 |
T2936 |
T2938 |
nummod |
0.5,DTT |
R2576 |
T2937 |
T2938 |
compound |
mM,DTT |
R2577 |
T2938 |
T2934 |
conj |
DTT,EDTA |
R2578 |
T2939 |
T2938 |
punct |
",",DTT |
R2579 |
T2940 |
T2938 |
cc |
and,DTT |
R2580 |
T2941 |
T2942 |
nummod |
2,μg |
R2581 |
T2942 |
T2944 |
compound |
μg,dI-dC |
R2582 |
T2943 |
T2944 |
compound |
poly,dI-dC |
R2583 |
T2944 |
T2934 |
appos |
dI-dC,EDTA |
R2584 |
T2945 |
T2944 |
prep |
for,dI-dC |
R2585 |
T2946 |
T2947 |
nummod |
20,minutes |
R2586 |
T2947 |
T2945 |
pobj |
minutes,for |
R2587 |
T2948 |
T2947 |
prep |
on,minutes |
R2588 |
T2949 |
T2948 |
pobj |
ice,on |
R2589 |
T2950 |
T2926 |
punct |
.,NaCl |
R2590 |
T2951 |
T2964 |
prep |
After,continued |
R2591 |
T2952 |
T2951 |
pobj |
addition,After |
R2592 |
T2953 |
T2952 |
prep |
of,addition |
R2593 |
T2954 |
T2959 |
det |
the,probe |
R2594 |
T2955 |
T2956 |
nummod |
1,μL |
R2595 |
T2956 |
T2959 |
nmod |
μL,probe |
R2596 |
T2957 |
T2959 |
amod |
32P-labled,probe |
R2597 |
T2958 |
T2959 |
compound |
oligonucleotide,probe |
R2598 |
T2959 |
T2953 |
pobj |
probe,of |
R2599 |
T2960 |
T2964 |
punct |
",",continued |
R2600 |
T2961 |
T2962 |
det |
the,incubation |
R2601 |
T2962 |
T2964 |
nsubjpass |
incubation,continued |
R2602 |
T2963 |
T2964 |
auxpass |
was,continued |
R2603 |
T2964 |
T2964 |
ROOT |
continued,continued |
R2604 |
T2965 |
T2964 |
prep |
for,continued |
R2605 |
T2966 |
T2967 |
nummod |
20,minutes |
R2606 |
T2967 |
T2965 |
pobj |
minutes,for |
R2607 |
T2968 |
T2967 |
prep |
on,minutes |
R2608 |
T2969 |
T2968 |
pobj |
ice,on |
R2609 |
T2970 |
T2964 |
punct |
.,continued |
R2610 |
T2971 |
T2973 |
det |
The,complexes |
R2611 |
T2972 |
T2973 |
amod |
DNA-protein,complexes |
R2612 |
T2973 |
T2975 |
nsubjpass |
complexes,separated |
R2613 |
T2974 |
T2975 |
auxpass |
were,separated |
R2614 |
T2975 |
T2975 |
ROOT |
separated,separated |
R2615 |
T2976 |
T2975 |
agent |
by,separated |
R2616 |
T2977 |
T2976 |
pobj |
electrophoresis,by |
R2617 |
T2978 |
T2975 |
prep |
on,separated |
R2618 |
T2979 |
T2980 |
nummod |
4,% |
R2619 |
T2980 |
T2983 |
nmod |
%,gel |
R2620 |
T2981 |
T2983 |
amod |
nondenaturing,gel |
R2621 |
T2982 |
T2983 |
compound |
polyacrylamide,gel |
R2622 |
T2983 |
T2978 |
pobj |
gel,on |
R2623 |
T2984 |
T2975 |
prep |
in,separated |
R2624 |
T2985 |
T2988 |
nummod |
0.5,buffer |
R2625 |
T2986 |
T2988 |
nummod |
×,buffer |
R2626 |
T2987 |
T2988 |
compound |
TBE,buffer |
R2627 |
T2988 |
T2984 |
pobj |
buffer,in |
R2628 |
T2989 |
T2988 |
punct |
(,buffer |
R2629 |
T2990 |
T2988 |
appos |
tris-borate-EDTA,buffer |
R2630 |
T2991 |
T2988 |
punct |
),buffer |
R2631 |
T2992 |
T2975 |
prep |
at,separated |
R2632 |
T2993 |
T2994 |
nummod |
390,V |
R2633 |
T2994 |
T2992 |
pobj |
V,at |
R2634 |
T2995 |
T2975 |
prep |
for,separated |
R2635 |
T2996 |
T2997 |
nummod |
1,hour |
R2636 |
T2997 |
T2995 |
pobj |
hour,for |
R2637 |
T2998 |
T2975 |
prep |
at,separated |
R2638 |
T2999 |
T3000 |
nummod |
4,° |
R2639 |
T3000 |
T2998 |
pobj |
°,at |
R2640 |
T3001 |
T2975 |
npadvmod |
C,separated |
R2641 |
T3002 |
T2975 |
punct |
.,separated |
R2642 |
T3003 |
T3009 |
prep |
After,dried |
R2643 |
T3004 |
T3003 |
pobj |
electrophoresis,After |
R2644 |
T3005 |
T3009 |
punct |
",",dried |
R2645 |
T3006 |
T3007 |
det |
the,gel |
R2646 |
T3007 |
T3009 |
nsubjpass |
gel,dried |
R2647 |
T3008 |
T3009 |
auxpass |
was,dried |
R2648 |
T3009 |
T3009 |
ROOT |
dried,dried |
R2649 |
T3010 |
T3009 |
cc |
and,dried |
R2650 |
T3011 |
T3009 |
conj |
exposed,dried |
R2651 |
T3012 |
T3011 |
prep |
to,exposed |
R2652 |
T3013 |
T3014 |
compound |
X-ray,film |
R2653 |
T3014 |
T3012 |
pobj |
film,to |
R2654 |
T3015 |
T3017 |
punct |
(,Hyperfilm |
R2655 |
T3016 |
T3017 |
compound |
Fuji,Hyperfilm |
R2656 |
T3017 |
T3014 |
appos |
Hyperfilm,film |
R2657 |
T3018 |
T3017 |
punct |
",",Hyperfilm |
R2658 |
T3019 |
T3017 |
npadvmod |
Tokyo,Hyperfilm |
R2659 |
T3020 |
T3019 |
punct |
",",Tokyo |
R2660 |
T3021 |
T3019 |
conj |
Japan,Tokyo |
R2661 |
T3022 |
T3017 |
punct |
),Hyperfilm |
R2662 |
T3023 |
T3009 |
punct |
.,dried |
R2663 |
T3024 |
T3031 |
nsubjpass |
Levels,quantified |
R2664 |
T3025 |
T3024 |
prep |
of,Levels |
R2665 |
T3026 |
T3027 |
compound |
NF-κB,DNA |
R2666 |
T3027 |
T3025 |
pobj |
DNA,of |
R2667 |
T3028 |
T3029 |
amod |
binding,activity |
R2668 |
T3029 |
T3025 |
pobj |
activity,of |
R2669 |
T3030 |
T3031 |
auxpass |
were,quantified |
R2670 |
T3031 |
T3031 |
ROOT |
quantified,quantified |
R2671 |
T3032 |
T3031 |
agent |
by,quantified |
R2672 |
T3033 |
T3034 |
compound |
software,ImageJ |
R2673 |
T3034 |
T3032 |
pobj |
ImageJ,by |
R2674 |
T3035 |
T3031 |
punct |
.,quantified |