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PMC:3317373 / 10541-11434 JSONTXT 19 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4560 0-3 CD denotes 2.8
T4561 3-4 . denotes .
T4562 5-13 NNP denotes Gelatine
T4563 14-24 NNP denotes Zymography
T4564 25-30 NNP denotes Cells
T4565 31-33 IN denotes of
T4566 34-38 CD denotes four
T4567 39-45 NNS denotes groups
T4568 46-50 VBD denotes were
T4569 51-62 VBN denotes homogenized
T4570 63-65 IN denotes in
T4571 66-71 NN denotes lysis
T4572 72-78 NN denotes buffer
T4573 79-89 VBG denotes containing
T4574 90-92 CD denotes 50
T4575 93-95 NNP denotes mM
T4576 96-104 NNP denotes Tris-HCl
T4577 105-106 -LRB- denotes (
T4578 106-108 NNP denotes pH
T4579 109-112 CD denotes 7.4
T4580 112-113 -RRB- denotes )
T4581 113-114 , denotes ,
T4582 115-118 CD denotes 150
T4583 119-121 NNP denotes mM
T4584 122-126 NNP denotes NaCl
T4585 126-127 , denotes ,
T4586 128-129 CD denotes 5
T4587 130-132 NNP denotes mM
T4588 133-138 NNP denotes CaCl2
T4589 138-139 , denotes ,
T4590 140-143 CD denotes 0.2
T4591 144-146 NNP denotes mM
T4592 147-151 NNP denotes NaN3
T4593 151-152 , denotes ,
T4594 153-156 CC denotes and
T4595 157-161 CD denotes 0.01
T4596 161-162 NN denotes %
T4597 163-169 NNP denotes Triton
T4598 169-170 . denotes .
T4599 171-178 JJ denotes Soluble
T4600 179-187 NNS denotes extracts
T4601 188-192 VBD denotes were
T4602 193-202 VBN denotes separated
T4603 203-205 IN denotes by
T4604 206-220 NN denotes centrifugation
T4605 221-224 CC denotes and
T4606 225-231 VBD denotes stored
T4607 232-234 IN denotes at
T4608 236-238 CD denotes 20
T4609 238-239 NN denotes °
T4610 239-241 NNP denotes C.
T4611 242-249 NNP denotes Gelatin
T4612 250-260 NN denotes zymography
T4613 261-264 VBD denotes was
T4614 265-274 VBN denotes performed
T4615 275-284 VBG denotes according
T4616 285-287 TO denotes to
T4617 288-291 DT denotes the
T4618 292-304 NN denotes manufacturer
T4619 304-306 POS denotes 's
T4620 307-319 NNS denotes instructions
T4621 320-321 -LRB- denotes (
T4622 321-327 NNP denotes Genmed
T4623 328-339 NNP denotes Scientifics
T4624 340-343 NNP denotes Inc
T4625 343-344 , denotes ,
T4626 345-347 NNP denotes MA
T4627 347-348 , denotes ,
T4628 349-352 NNP denotes USA
T4629 352-353 -RRB- denotes )
T4630 353-354 . denotes .
T4631 355-362 RB denotes Briefly
T4632 362-363 , denotes ,
T4633 364-366 CD denotes 40
T4634 367-369 NN denotes μg
T4635 370-379 JJ denotes cytosolic
T4636 380-387 NN denotes protein
T4637 388-396 NNS denotes extracts
T4638 397-401 VBD denotes were
T4639 402-411 VBN denotes separated
T4640 412-414 IN denotes by
T4641 415-430 NN denotes electrophoresis
T4642 430-431 . denotes .
T4643 432-436 RB denotes Then
T4644 437-440 DT denotes the
T4645 441-449 NNS denotes proteins
T4646 450-454 VBD denotes were
T4647 455-464 VBN denotes renatured
T4648 465-467 IN denotes by
T4649 468-478 NN denotes incubation
T4650 479-481 IN denotes in
T4651 482-485 CD denotes 2.5
T4652 485-486 NN denotes %
T4653 487-493 NNP denotes Triton
T4654 494-499 NNP denotes X-100
T4655 500-503 CC denotes and
T4656 504-508 RB denotes then
T4657 509-518 VBD denotes incubated
T4658 519-521 IN denotes in
T4659 522-531 JJ denotes substrate
T4660 532-538 NN denotes buffer
T4661 539-542 IN denotes for
T4662 543-545 CD denotes 40
T4663 546-547 NN denotes h
T4664 548-550 IN denotes at
T4665 551-553 CD denotes 37
T4666 553-554 CD denotes °
T4667 554-555 NNP denotes C
T4668 556-558 TO denotes to
T4669 559-565 VB denotes enable
T4670 566-569 DT denotes the
T4671 570-574 NNP denotes MMP9
T4672 575-577 TO denotes to
T4673 578-584 VB denotes cleave
T4674 585-588 DT denotes the
T4675 589-596 NN denotes gelatin
T4676 596-597 . denotes .
T4677 598-603 IN denotes After
T4678 604-611 VBG denotes rinsing
T4679 612-614 IN denotes in
T4680 615-620 NN denotes water
T4681 620-621 , denotes ,
T4682 622-626 DT denotes each
T4683 627-630 NN denotes gel
T4684 631-634 VBD denotes was
T4685 635-642 VBN denotes stained
T4686 643-647 IN denotes with
T4687 648-657 NNP denotes Coomassie
T4688 658-662 JJ denotes blue
T4689 663-666 IN denotes for
T4690 667-668 CD denotes 1
T4691 669-670 NN denotes h
T4692 671-674 CC denotes and
T4693 675-684 VBN denotes destained
T4694 685-687 IN denotes in
T4695 688-690 CD denotes 50
T4696 690-691 NN denotes %
T4697 692-700 NN denotes methanol
T4698 700-701 . denotes .
T4699 702-713 JJ denotes Proteolytic
T4700 714-724 NNS denotes activities
T4701 725-729 VBD denotes were
T4702 730-736 VBN denotes showed
T4703 737-739 IN denotes by
T4704 740-745 JJ denotes clear
T4705 746-751 NNS denotes bands
T4706 752-754 IN denotes in
T4707 755-759 JJ denotes blue
T4708 760-763 NN denotes gel
T4709 764-769 WDT denotes which
T4710 770-779 VBZ denotes indicates
T4711 780-783 DT denotes the
T4712 784-789 NN denotes lysis
T4713 790-792 IN denotes of
T4714 793-796 DT denotes the
T4715 797-806 NN denotes substrate
T4716 806-807 . denotes .
T4717 808-822 NN denotes Quantification
T4718 823-825 IN denotes of
T4719 826-830 NNP denotes MMP9
T4720 831-835 NN denotes band
T4721 836-843 NN denotes density
T4722 844-847 VBD denotes was
T4723 848-857 VBN denotes performed
T4724 858-862 IN denotes with
T4725 863-868 NN denotes image
T4726 869-877 NN denotes analysis
T4727 878-885 NN denotes program
T4728 886-892 NNP denotes ImageJ
T4729 892-893 . denotes .
R3873 T4560 T4560 ROOT 2.8,2.8
R3874 T4561 T4560 punct .,2.8
R3875 T4562 T4564 compound Gelatine,Cells
R3876 T4563 T4564 compound Zymography,Cells
R3877 T4564 T4569 nsubjpass Cells,homogenized
R3878 T4565 T4564 prep of,Cells
R3879 T4566 T4567 nummod four,groups
R3880 T4567 T4565 pobj groups,of
R3881 T4568 T4569 auxpass were,homogenized
R3882 T4569 T4569 ROOT homogenized,homogenized
R3883 T4570 T4569 prep in,homogenized
R3884 T4571 T4572 compound lysis,buffer
R3885 T4572 T4570 pobj buffer,in
R3886 T4573 T4572 acl containing,buffer
R3887 T4574 T4576 nummod 50,Tris-HCl
R3888 T4575 T4576 compound mM,Tris-HCl
R3889 T4576 T4573 dobj Tris-HCl,containing
R3890 T4577 T4578 punct (,pH
R3891 T4578 T4576 appos pH,Tris-HCl
R3892 T4579 T4578 nummod 7.4,pH
R3893 T4580 T4578 punct ),pH
R3894 T4581 T4576 punct ",",Tris-HCl
R3895 T4582 T4584 nummod 150,NaCl
R3896 T4583 T4584 compound mM,NaCl
R3897 T4584 T4576 conj NaCl,Tris-HCl
R3898 T4585 T4584 punct ",",NaCl
R3899 T4586 T4588 nummod 5,CaCl2
R3900 T4587 T4588 compound mM,CaCl2
R3901 T4588 T4584 conj CaCl2,NaCl
R3902 T4589 T4588 punct ",",CaCl2
R3903 T4590 T4592 nummod 0.2,NaN3
R3904 T4591 T4592 compound mM,NaN3
R3905 T4592 T4588 conj NaN3,CaCl2
R3906 T4593 T4592 punct ",",NaN3
R3907 T4594 T4592 cc and,NaN3
R3908 T4595 T4596 nummod 0.01,%
R3909 T4596 T4597 compound %,Triton
R3910 T4597 T4592 conj Triton,NaN3
R3911 T4598 T4569 punct .,homogenized
R3912 T4599 T4600 amod Soluble,extracts
R3913 T4600 T4602 nsubjpass extracts,separated
R3914 T4601 T4602 auxpass were,separated
R3915 T4602 T4602 ROOT separated,separated
R3916 T4603 T4602 agent by,separated
R3917 T4604 T4603 pobj centrifugation,by
R3918 T4605 T4602 cc and,separated
R3919 T4606 T4614 nsubjpass stored,performed
R3920 T4607 T4606 prep at,stored
R3921 T4608 T4609 nummod 20,°
R3922 T4609 T4612 compound °,zymography
R3923 T4610 T4612 compound C.,zymography
R3924 T4611 T4612 compound Gelatin,zymography
R3925 T4612 T4607 pobj zymography,at
R3926 T4613 T4614 auxpass was,performed
R3927 T4614 T4602 conj performed,separated
R3928 T4615 T4614 xcomp according,performed
R3929 T4616 T4615 prep to,according
R3930 T4617 T4618 det the,manufacturer
R3931 T4618 T4620 poss manufacturer,instructions
R3932 T4619 T4618 case 's,manufacturer
R3933 T4620 T4616 pobj instructions,to
R3934 T4621 T4624 punct (,Inc
R3935 T4622 T4624 compound Genmed,Inc
R3936 T4623 T4624 compound Scientifics,Inc
R3937 T4624 T4620 appos Inc,instructions
R3938 T4625 T4624 punct ",",Inc
R3939 T4626 T4624 conj MA,Inc
R3940 T4627 T4626 punct ",",MA
R3941 T4628 T4626 appos USA,MA
R3942 T4629 T4626 punct ),MA
R3943 T4630 T4614 punct .,performed
R3944 T4631 T4639 advmod Briefly,separated
R3945 T4632 T4639 punct ",",separated
R3946 T4633 T4637 nummod 40,extracts
R3947 T4634 T4637 compound μg,extracts
R3948 T4635 T4636 compound cytosolic,protein
R3949 T4636 T4637 compound protein,extracts
R3950 T4637 T4639 nsubjpass extracts,separated
R3951 T4638 T4639 auxpass were,separated
R3952 T4639 T4639 ROOT separated,separated
R3953 T4640 T4639 prep by,separated
R3954 T4641 T4640 pobj electrophoresis,by
R3955 T4642 T4639 punct .,separated
R3956 T4643 T4647 advmod Then,renatured
R3957 T4644 T4645 det the,proteins
R3958 T4645 T4647 nsubjpass proteins,renatured
R3959 T4646 T4647 auxpass were,renatured
R3960 T4647 T4647 ROOT renatured,renatured
R3961 T4648 T4647 agent by,renatured
R3962 T4649 T4648 pobj incubation,by
R3963 T4650 T4647 prep in,renatured
R3964 T4651 T4652 nummod 2.5,%
R3965 T4652 T4654 compound %,X-100
R3966 T4653 T4654 compound Triton,X-100
R3967 T4654 T4650 pobj X-100,in
R3968 T4655 T4647 cc and,renatured
R3969 T4656 T4657 advmod then,incubated
R3970 T4657 T4647 conj incubated,renatured
R3971 T4658 T4657 prep in,incubated
R3972 T4659 T4660 compound substrate,buffer
R3973 T4660 T4658 pobj buffer,in
R3974 T4661 T4660 prep for,buffer
R3975 T4662 T4663 nummod 40,h
R3976 T4663 T4661 pobj h,for
R3977 T4664 T4657 prep at,incubated
R3978 T4665 T4666 nummod 37,°
R3979 T4666 T4664 pobj °,at
R3980 T4667 T4657 conj C,incubated
R3981 T4668 T4669 aux to,enable
R3982 T4669 T4657 advcl enable,incubated
R3983 T4670 T4671 det the,MMP9
R3984 T4671 T4669 dobj MMP9,enable
R3985 T4672 T4673 aux to,cleave
R3986 T4673 T4669 xcomp cleave,enable
R3987 T4674 T4675 det the,gelatin
R3988 T4675 T4673 dobj gelatin,cleave
R3989 T4676 T4647 punct .,renatured
R3990 T4677 T4685 prep After,stained
R3991 T4678 T4677 pcomp rinsing,After
R3992 T4679 T4678 prep in,rinsing
R3993 T4680 T4679 pobj water,in
R3994 T4681 T4685 punct ",",stained
R3995 T4682 T4683 det each,gel
R3996 T4683 T4685 nsubjpass gel,stained
R3997 T4684 T4685 auxpass was,stained
R3998 T4685 T4685 ROOT stained,stained
R3999 T4686 T4685 prep with,stained
R4000 T4687 T4688 compound Coomassie,blue
R4001 T4688 T4686 pobj blue,with
R4002 T4689 T4688 prep for,blue
R4003 T4690 T4691 nummod 1,h
R4004 T4691 T4689 pobj h,for
R4005 T4692 T4691 cc and,h
R4006 T4693 T4688 conj destained,blue
R4007 T4694 T4693 prep in,destained
R4008 T4695 T4696 nummod 50,%
R4009 T4696 T4697 compound %,methanol
R4010 T4697 T4694 pobj methanol,in
R4011 T4698 T4685 punct .,stained
R4012 T4699 T4700 amod Proteolytic,activities
R4013 T4700 T4702 nsubjpass activities,showed
R4014 T4701 T4702 auxpass were,showed
R4015 T4702 T4702 ROOT showed,showed
R4016 T4703 T4702 agent by,showed
R4017 T4704 T4705 amod clear,bands
R4018 T4705 T4703 pobj bands,by
R4019 T4706 T4705 prep in,bands
R4020 T4707 T4708 amod blue,gel
R4021 T4708 T4706 pobj gel,in
R4022 T4709 T4710 nsubj which,indicates
R4023 T4710 T4702 advcl indicates,showed
R4024 T4711 T4712 det the,lysis
R4025 T4712 T4710 dobj lysis,indicates
R4026 T4713 T4712 prep of,lysis
R4027 T4714 T4715 det the,substrate
R4028 T4715 T4713 pobj substrate,of
R4029 T4716 T4702 punct .,showed
R4030 T4717 T4723 nsubjpass Quantification,performed
R4031 T4718 T4717 prep of,Quantification
R4032 T4719 T4721 compound MMP9,density
R4033 T4720 T4721 compound band,density
R4034 T4721 T4718 pobj density,of
R4035 T4722 T4723 auxpass was,performed
R4036 T4723 T4723 ROOT performed,performed
R4037 T4724 T4723 prep with,performed
R4038 T4725 T4726 compound image,analysis
R4039 T4726 T4727 compound analysis,program
R4040 T4727 T4728 compound program,ImageJ
R4041 T4728 T4724 pobj ImageJ,with
R4042 T4729 T4723 punct .,performed