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PMC:3312845 / 10129-11629 JSONTXT 11 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5059 0-13 JJ denotes Free-floating
T5060 14-22 NNS denotes sections
T5061 23-27 VBD denotes were
T5062 28-33 JJ denotes first
T5063 34-43 VBN denotes incubated
T5064 44-48 IN denotes with
T5065 49-51 CD denotes 10
T5066 51-52 NN denotes %
T5067 53-59 JJ denotes normal
T5068 60-64 NN denotes goat
T5069 65-70 NN denotes serum
T5070 71-74 IN denotes for
T5071 75-77 CD denotes 30
T5072 78-81 NN denotes min
T5073 82-84 IN denotes at
T5074 85-89 NN denotes room
T5075 90-101 NN denotes temperature
T5076 101-102 . denotes .
T5077 103-107 PRP denotes They
T5078 108-112 VBD denotes were
T5079 113-117 RB denotes then
T5080 118-127 VBN denotes incubated
T5081 128-130 IN denotes in
T5082 131-137 NN denotes rabbit
T5083 138-146 JJ denotes anti-MPO
T5084 147-150 NNP denotes IgG
T5085 151-152 -LRB- denotes (
T5086 152-157 CD denotes 1:100
T5087 157-158 , denotes ,
T5088 159-165 NNP denotes Thermo
T5089 166-172 NN denotes fisher
T5090 173-183 JJ denotes scientific
T5091 183-184 -RRB- denotes )
T5092 185-187 CC denotes or
T5093 188-194 NN denotes rabbit
T5094 195-205 JJ denotes anti-MIP-2
T5095 206-209 NNP denotes IgG
T5096 210-211 -LRB- denotes (
T5097 211-216 CD denotes 1:200
T5098 216-217 , denotes ,
T5099 218-228 NNP denotes Invitrogen
T5100 228-229 , denotes ,
T5101 230-238 NNP denotes Carlsbad
T5102 238-239 , denotes ,
T5103 240-242 NNP denotes CA
T5104 242-243 -RRB- denotes )
T5105 244-246 IN denotes in
T5106 247-250 NNP denotes PBS
T5107 251-261 VBG denotes containing
T5108 262-265 CD denotes 0.3
T5109 265-266 NN denotes %
T5110 267-273 NNP denotes Triton
T5111 274-279 NNP denotes X-100
T5112 280-281 -LRB- denotes (
T5113 281-294 NNP denotes Sigma-Aldrich
T5114 295-298 NNP denotes Co.
T5115 298-299 , denotes ,
T5116 300-303 NNP denotes St.
T5117 304-309 NNP denotes Louis
T5118 309-310 , denotes ,
T5119 311-313 NNP denotes MO
T5120 313-314 -RRB- denotes )
T5121 315-318 CC denotes and
T5122 319-320 CD denotes 2
T5123 320-321 NN denotes %
T5124 322-328 JJ denotes normal
T5125 329-333 NN denotes goat
T5126 334-339 NN denotes serum
T5127 339-340 -LRB- denotes (
T5128 340-353 NNP denotes Sigma-Aldrich
T5129 354-357 NNP denotes Co.
T5130 357-358 , denotes ,
T5131 359-362 NNP denotes St.
T5132 363-368 NNP denotes Louis
T5133 368-369 , denotes ,
T5134 370-372 NNP denotes MO
T5135 372-373 -RRB- denotes )
T5136 374-383 RB denotes overnight
T5137 384-386 IN denotes at
T5138 387-391 NN denotes room
T5139 392-403 NN denotes temperature
T5140 403-404 . denotes .
T5141 405-410 IN denotes After
T5142 411-418 VBG denotes washing
T5143 419-424 CD denotes three
T5144 425-430 NNS denotes times
T5145 431-434 IN denotes for
T5146 435-437 CD denotes 10
T5147 438-441 NN denotes min
T5148 442-446 IN denotes with
T5149 447-450 NNP denotes PBS
T5150 450-451 , denotes ,
T5151 452-455 DT denotes the
T5152 456-464 NNS denotes sections
T5153 465-469 VBD denotes were
T5154 470-479 VBN denotes incubated
T5155 480-492 RB denotes sequentially
T5156 492-493 , denotes ,
T5157 494-496 IN denotes in
T5158 497-501 NN denotes goat
T5159 502-513 JJ denotes anti-rabbit
T5160 514-516 CC denotes or
T5161 517-522 NN denotes horse
T5162 523-533 JJ denotes anti-mouse
T5163 534-537 NNP denotes IgG
T5164 538-539 -LRB- denotes (
T5165 539-545 NNP denotes Vector
T5166 545-546 , denotes ,
T5167 547-557 NNP denotes Burlingame
T5168 557-558 , denotes ,
T5169 559-561 NNP denotes CA
T5170 561-562 -RRB- denotes )
T5171 563-566 CC denotes and
T5172 567-570 NNP denotes ABC
T5173 571-578 NN denotes complex
T5174 579-580 -LRB- denotes (
T5175 580-586 NNP denotes Vector
T5176 586-587 , denotes ,
T5177 588-598 NNP denotes Burlingame
T5178 598-599 , denotes ,
T5179 600-602 NNP denotes CA
T5180 602-603 -RRB- denotes )
T5181 603-604 , denotes ,
T5182 605-612 VBN denotes diluted
T5183 613-618 CD denotes 1:200
T5184 619-621 IN denotes in
T5185 622-625 DT denotes the
T5186 626-630 JJ denotes same
T5187 631-639 NN denotes solution
T5188 640-642 IN denotes as
T5189 643-646 DT denotes the
T5190 647-654 JJ denotes primary
T5191 655-664 NN denotes antiserum
T5192 664-665 . denotes .
T5193 666-673 IN denotes Between
T5194 674-677 DT denotes the
T5195 678-689 NNS denotes incubations
T5196 689-690 , denotes ,
T5197 691-694 DT denotes the
T5198 695-702 NNS denotes tissues
T5199 703-707 VBD denotes were
T5200 708-714 VBN denotes washed
T5201 715-719 IN denotes with
T5202 720-723 NNP denotes PBS
T5203 724-729 CD denotes three
T5204 730-735 NNS denotes times
T5205 736-739 IN denotes for
T5206 740-742 CD denotes 10
T5207 743-746 NN denotes min
T5208 747-751 DT denotes each
T5209 751-752 . denotes .
T5210 753-755 TO denotes To
T5211 756-763 VB denotes confirm
T5212 764-773 JJ denotes vasogenic
T5213 774-779 NN denotes edema
T5214 779-780 , denotes ,
T5215 781-785 DT denotes some
T5216 786-792 NN denotes tissue
T5217 793-801 NNS denotes sections
T5218 802-806 VBD denotes were
T5219 807-814 VBN denotes reacted
T5220 815-818 IN denotes for
T5221 819-833 NNS denotes serum-proteins
T5222 834-839 VBG denotes using
T5223 840-845 NN denotes horse
T5224 846-854 JJ denotes anti-rat
T5225 855-858 NNP denotes IgG
T5226 859-860 -LRB- denotes (
T5227 860-866 NNP denotes Vector
T5228 866-867 , denotes ,
T5229 868-878 NNP denotes Burlingame
T5230 878-879 , denotes ,
T5231 880-882 NNP denotes CA
T5232 882-883 -RRB- denotes )
T5233 884-886 IN denotes as
T5234 887-888 DT denotes a
T5235 889-896 JJ denotes primary
T5236 897-905 NN denotes antibody
T5237 905-906 . denotes .
T5238 907-910 DT denotes The
T5239 911-919 NNS denotes sections
T5240 920-924 VBD denotes were
T5241 925-935 VBN denotes visualized
T5242 936-940 IN denotes with
T5243 941-944 CD denotes 3,3
T5244 944-945 '' denotes '
T5245 945-946 : denotes -
T5246 946-962 NN denotes diaminobenzidine
T5247 963-964 -LRB- denotes (
T5248 964-967 NNP denotes DAB
T5249 967-968 , denotes ,
T5250 969-982 NNP denotes Sigma-Aldrich
T5251 983-986 NNP denotes Co.
T5252 986-987 , denotes ,
T5253 988-991 NNP denotes St.
T5254 992-997 NNP denotes Louis
T5255 997-998 , denotes ,
T5256 999-1001 NNP denotes MO
T5257 1001-1002 -RRB- denotes )
T5258 1003-1005 IN denotes in
T5259 1006-1009 CD denotes 0.1
T5260 1010-1011 NNP denotes M
T5261 1012-1016 NNP denotes Tris
T5262 1017-1023 NN denotes buffer
T5263 1024-1027 CC denotes and
T5264 1028-1035 VBD denotes mounted
T5265 1036-1038 IN denotes on
T5266 1039-1042 DT denotes the
T5267 1043-1057 JJ denotes gelatin-coated
T5268 1058-1064 NNS denotes slides
T5269 1064-1065 . denotes .
T5270 1066-1069 DT denotes The
T5271 1070-1085 NNS denotes immunoreactions
T5272 1086-1090 VBD denotes were
T5273 1091-1099 VBN denotes observed
T5274 1100-1105 IN denotes under
T5275 1106-1109 DT denotes the
T5276 1110-1119 NNP denotes Axioscope
T5277 1120-1130 NN denotes microscope
T5278 1131-1132 -LRB- denotes (
T5279 1132-1136 NNP denotes Carl
T5280 1137-1142 NNP denotes Zeiss
T5281 1142-1143 , denotes ,
T5282 1144-1164 NNP denotes Munchen-Hallbergmoos
T5283 1164-1165 -RRB- denotes )
T5284 1165-1166 . denotes .
T5285 1167-1170 IN denotes For
T5286 1171-1179 JJ denotes negative
T5287 1180-1188 NNS denotes controls
T5288 1188-1189 , denotes ,
T5289 1190-1193 NN denotes rat
T5290 1194-1205 JJ denotes hippocampal
T5291 1206-1213 NNS denotes tissues
T5292 1214-1218 VBD denotes were
T5293 1219-1228 VBN denotes incubated
T5294 1229-1233 IN denotes with
T5295 1234-1235 CD denotes 1
T5296 1236-1238 NN denotes μg
T5297 1239-1241 IN denotes of
T5298 1242-1245 DT denotes the
T5299 1246-1254 NN denotes antibody
T5300 1255-1259 WDT denotes that
T5301 1260-1263 VBD denotes was
T5302 1264-1276 VBN denotes preincubated
T5303 1277-1281 IN denotes with
T5304 1282-1283 CD denotes 1
T5305 1284-1286 NN denotes μg
T5306 1287-1289 IN denotes of
T5307 1290-1298 JJ denotes purified
T5308 1299-1306 NN denotes peptide
T5309 1307-1310 IN denotes for
T5310 1311-1312 CD denotes 1
T5311 1313-1315 NN denotes hr
T5312 1316-1318 IN denotes at
T5313 1319-1323 NN denotes room
T5314 1324-1335 NN denotes temperature
T5315 1336-1338 CC denotes or
T5316 1339-1348 VBN denotes incubated
T5317 1349-1353 IN denotes with
T5318 1354-1364 JJ denotes pre-immune
T5319 1365-1370 NN denotes serum
T5320 1371-1378 RB denotes instead
T5321 1379-1381 IN denotes of
T5322 1382-1385 DT denotes the
T5323 1386-1393 JJ denotes primary
T5324 1394-1402 NN denotes antibody
T5325 1402-1403 . denotes .
T5326 1404-1407 IN denotes For
T5327 1408-1416 JJ denotes negative
T5328 1417-1425 NNS denotes controls
T5329 1425-1426 , denotes ,
T5330 1427-1434 NNS denotes tissues
T5331 1435-1439 VBD denotes were
T5332 1440-1449 VBN denotes incubated
T5333 1450-1454 IN denotes with
T5334 1455-1465 JJ denotes pre-immune
T5335 1466-1471 NN denotes serum
T5336 1472-1479 RB denotes instead
T5337 1480-1482 IN denotes of
T5338 1483-1490 JJ denotes primary
T5339 1491-1499 NN denotes antibody
T5340 1499-1500 . denotes .
R4061 T5059 T5060 amod Free-floating,sections
R4062 T5060 T5063 nsubjpass sections,incubated
R4063 T5061 T5063 auxpass were,incubated
R4064 T5062 T5063 advmod first,incubated
R4065 T5063 T5063 ROOT incubated,incubated
R4066 T5064 T5063 prep with,incubated
R4067 T5065 T5066 nummod 10,%
R4068 T5066 T5069 nmod %,serum
R4069 T5067 T5069 amod normal,serum
R4070 T5068 T5069 compound goat,serum
R4071 T5069 T5064 pobj serum,with
R4072 T5070 T5069 prep for,serum
R4073 T5071 T5072 nummod 30,min
R4074 T5072 T5070 pobj min,for
R4075 T5073 T5072 prep at,min
R4076 T5074 T5075 compound room,temperature
R4077 T5075 T5073 pobj temperature,at
R4078 T5076 T5063 punct .,incubated
R4079 T5077 T5080 nsubjpass They,incubated
R4080 T5078 T5080 auxpass were,incubated
R4081 T5079 T5080 advmod then,incubated
R4082 T5080 T5080 ROOT incubated,incubated
R4083 T5081 T5080 prep in,incubated
R4084 T5082 T5084 compound rabbit,IgG
R4085 T5083 T5084 compound anti-MPO,IgG
R4086 T5084 T5081 pobj IgG,in
R4087 T5085 T5084 punct (,IgG
R4088 T5086 T5084 appos 1:100,IgG
R4089 T5087 T5084 punct ",",IgG
R4090 T5088 T5095 nmod Thermo,IgG
R4091 T5089 T5090 advmod fisher,scientific
R4092 T5090 T5088 amod scientific,Thermo
R4093 T5091 T5090 punct ),scientific
R4094 T5092 T5090 cc or,scientific
R4095 T5093 T5090 conj rabbit,scientific
R4096 T5094 T5095 amod anti-MIP-2,IgG
R4097 T5095 T5084 appos IgG,IgG
R4098 T5096 T5095 punct (,IgG
R4099 T5097 T5095 appos 1:200,IgG
R4100 T5098 T5095 punct ",",IgG
R4101 T5099 T5095 conj Invitrogen,IgG
R4102 T5100 T5099 punct ",",Invitrogen
R4103 T5101 T5099 conj Carlsbad,Invitrogen
R4104 T5102 T5101 punct ",",Carlsbad
R4105 T5103 T5101 conj CA,Carlsbad
R4106 T5104 T5101 punct ),Carlsbad
R4107 T5105 T5095 prep in,IgG
R4108 T5106 T5105 pobj PBS,in
R4109 T5107 T5080 advcl containing,incubated
R4110 T5108 T5109 nummod 0.3,%
R4111 T5109 T5111 compound %,X-100
R4112 T5110 T5111 compound Triton,X-100
R4113 T5111 T5107 dobj X-100,containing
R4114 T5112 T5114 punct (,Co.
R4115 T5113 T5114 compound Sigma-Aldrich,Co.
R4116 T5114 T5111 appos Co.,X-100
R4117 T5115 T5114 punct ",",Co.
R4118 T5116 T5117 compound St.,Louis
R4119 T5117 T5114 npadvmod Louis,Co.
R4120 T5118 T5114 punct ",",Co.
R4121 T5119 T5114 npadvmod MO,Co.
R4122 T5120 T5114 punct ),Co.
R4123 T5121 T5111 cc and,X-100
R4124 T5122 T5123 nummod 2,%
R4125 T5123 T5126 nmod %,serum
R4126 T5124 T5126 amod normal,serum
R4127 T5125 T5126 compound goat,serum
R4128 T5126 T5111 conj serum,X-100
R4129 T5127 T5129 punct (,Co.
R4130 T5128 T5129 compound Sigma-Aldrich,Co.
R4131 T5129 T5126 appos Co.,serum
R4132 T5130 T5129 punct ",",Co.
R4133 T5131 T5132 compound St.,Louis
R4134 T5132 T5129 npadvmod Louis,Co.
R4135 T5133 T5129 punct ",",Co.
R4136 T5134 T5129 appos MO,Co.
R4137 T5135 T5129 punct ),Co.
R4138 T5136 T5107 advmod overnight,containing
R4139 T5137 T5136 prep at,overnight
R4140 T5138 T5139 compound room,temperature
R4141 T5139 T5137 pobj temperature,at
R4142 T5140 T5080 punct .,incubated
R4143 T5141 T5154 prep After,incubated
R4144 T5142 T5141 pcomp washing,After
R4145 T5143 T5144 nummod three,times
R4146 T5144 T5142 npadvmod times,washing
R4147 T5145 T5144 prep for,times
R4148 T5146 T5147 nummod 10,min
R4149 T5147 T5145 pobj min,for
R4150 T5148 T5147 prep with,min
R4151 T5149 T5148 pobj PBS,with
R4152 T5150 T5147 punct ",",min
R4153 T5151 T5152 det the,sections
R4154 T5152 T5154 nsubjpass sections,incubated
R4155 T5153 T5154 auxpass were,incubated
R4156 T5154 T5154 ROOT incubated,incubated
R4157 T5155 T5154 advmod sequentially,incubated
R4158 T5156 T5154 punct ",",incubated
R4159 T5157 T5154 prep in,incubated
R4160 T5158 T5157 pobj goat,in
R4161 T5159 T5163 nmod anti-rabbit,IgG
R4162 T5160 T5159 cc or,anti-rabbit
R4163 T5161 T5159 conj horse,anti-rabbit
R4164 T5162 T5163 amod anti-mouse,IgG
R4165 T5163 T5165 nmod IgG,Vector
R4166 T5164 T5165 punct (,Vector
R4167 T5165 T5165 ROOT Vector,Vector
R4168 T5166 T5165 punct ",",Vector
R4169 T5167 T5165 conj Burlingame,Vector
R4170 T5168 T5167 punct ",",Burlingame
R4171 T5169 T5167 conj CA,Burlingame
R4172 T5170 T5169 punct ),CA
R4173 T5171 T5167 cc and,Burlingame
R4174 T5172 T5173 compound ABC,complex
R4175 T5173 T5175 amod complex,Vector
R4176 T5174 T5175 punct (,Vector
R4177 T5175 T5165 conj Vector,Vector
R4178 T5176 T5175 punct ",",Vector
R4179 T5177 T5175 npadvmod Burlingame,Vector
R4180 T5178 T5177 punct ",",Burlingame
R4181 T5179 T5177 conj CA,Burlingame
R4182 T5180 T5177 punct ),Burlingame
R4183 T5181 T5175 punct ",",Vector
R4184 T5182 T5175 acl diluted,Vector
R4185 T5183 T5182 npadvmod 1:200,diluted
R4186 T5184 T5182 prep in,diluted
R4187 T5185 T5187 det the,solution
R4188 T5186 T5187 amod same,solution
R4189 T5187 T5184 pobj solution,in
R4190 T5188 T5187 prep as,solution
R4191 T5189 T5191 det the,antiserum
R4192 T5190 T5191 amod primary,antiserum
R4193 T5191 T5188 pobj antiserum,as
R4194 T5192 T5154 punct .,incubated
R4195 T5193 T5200 prep Between,washed
R4196 T5194 T5195 det the,incubations
R4197 T5195 T5193 pobj incubations,Between
R4198 T5196 T5200 punct ",",washed
R4199 T5197 T5198 det the,tissues
R4200 T5198 T5200 nsubjpass tissues,washed
R4201 T5199 T5200 auxpass were,washed
R4202 T5200 T5200 ROOT washed,washed
R4203 T5201 T5200 prep with,washed
R4204 T5202 T5201 pobj PBS,with
R4205 T5203 T5204 nummod three,times
R4206 T5204 T5200 npadvmod times,washed
R4207 T5205 T5200 prep for,washed
R4208 T5206 T5207 nummod 10,min
R4209 T5207 T5205 pobj min,for
R4210 T5208 T5207 appos each,min
R4211 T5209 T5200 punct .,washed
R4212 T5210 T5211 aux To,confirm
R4213 T5211 T5219 advcl confirm,reacted
R4214 T5212 T5213 amod vasogenic,edema
R4215 T5213 T5211 dobj edema,confirm
R4216 T5214 T5219 punct ",",reacted
R4217 T5215 T5217 det some,sections
R4218 T5216 T5217 compound tissue,sections
R4219 T5217 T5219 nsubjpass sections,reacted
R4220 T5218 T5219 auxpass were,reacted
R4221 T5219 T5219 ROOT reacted,reacted
R4222 T5220 T5219 prep for,reacted
R4223 T5221 T5220 pobj serum-proteins,for
R4224 T5222 T5219 advcl using,reacted
R4225 T5223 T5227 nmod horse,Vector
R4226 T5224 T5225 amod anti-rat,IgG
R4227 T5225 T5227 nmod IgG,Vector
R4228 T5226 T5227 punct (,Vector
R4229 T5227 T5222 dobj Vector,using
R4230 T5228 T5227 punct ",",Vector
R4231 T5229 T5227 conj Burlingame,Vector
R4232 T5230 T5229 punct ",",Burlingame
R4233 T5231 T5229 conj CA,Burlingame
R4234 T5232 T5227 punct ),Vector
R4235 T5233 T5222 prep as,using
R4236 T5234 T5236 det a,antibody
R4237 T5235 T5236 amod primary,antibody
R4238 T5236 T5233 pobj antibody,as
R4239 T5237 T5219 punct .,reacted
R4240 T5238 T5239 det The,sections
R4241 T5239 T5241 nsubjpass sections,visualized
R4242 T5240 T5241 auxpass were,visualized
R4243 T5241 T5241 ROOT visualized,visualized
R4244 T5242 T5241 prep with,visualized
R4245 T5243 T5246 nummod "3,3",diaminobenzidine
R4246 T5244 T5246 punct ',diaminobenzidine
R4247 T5245 T5246 punct -,diaminobenzidine
R4248 T5246 T5242 pobj diaminobenzidine,with
R4249 T5247 T5246 punct (,diaminobenzidine
R4250 T5248 T5246 appos DAB,diaminobenzidine
R4251 T5249 T5246 punct ",",diaminobenzidine
R4252 T5250 T5251 compound Sigma-Aldrich,Co.
R4253 T5251 T5246 conj Co.,diaminobenzidine
R4254 T5252 T5251 punct ",",Co.
R4255 T5253 T5254 compound St.,Louis
R4256 T5254 T5251 npadvmod Louis,Co.
R4257 T5255 T5251 punct ",",Co.
R4258 T5256 T5251 appos MO,Co.
R4259 T5257 T5251 punct ),Co.
R4260 T5258 T5246 prep in,diaminobenzidine
R4261 T5259 T5262 nummod 0.1,buffer
R4262 T5260 T5262 compound M,buffer
R4263 T5261 T5262 compound Tris,buffer
R4264 T5262 T5258 pobj buffer,in
R4265 T5263 T5241 cc and,visualized
R4266 T5264 T5241 conj mounted,visualized
R4267 T5265 T5264 prep on,mounted
R4268 T5266 T5268 det the,slides
R4269 T5267 T5268 amod gelatin-coated,slides
R4270 T5268 T5265 pobj slides,on
R4271 T5269 T5241 punct .,visualized
R4272 T5270 T5271 det The,immunoreactions
R4273 T5271 T5273 nsubjpass immunoreactions,observed
R4274 T5272 T5273 auxpass were,observed
R4275 T5273 T5273 ROOT observed,observed
R4276 T5274 T5273 prep under,observed
R4277 T5275 T5277 det the,microscope
R4278 T5276 T5277 compound Axioscope,microscope
R4279 T5277 T5274 pobj microscope,under
R4280 T5278 T5277 punct (,microscope
R4281 T5279 T5280 compound Carl,Zeiss
R4282 T5280 T5277 appos Zeiss,microscope
R4283 T5281 T5280 punct ",",Zeiss
R4284 T5282 T5280 appos Munchen-Hallbergmoos,Zeiss
R4285 T5283 T5280 punct ),Zeiss
R4286 T5284 T5273 punct .,observed
R4287 T5285 T5293 prep For,incubated
R4288 T5286 T5287 amod negative,controls
R4289 T5287 T5285 pobj controls,For
R4290 T5288 T5293 punct ",",incubated
R4291 T5289 T5291 nmod rat,tissues
R4292 T5290 T5291 amod hippocampal,tissues
R4293 T5291 T5293 nsubjpass tissues,incubated
R4294 T5292 T5293 auxpass were,incubated
R4295 T5293 T5293 ROOT incubated,incubated
R4296 T5294 T5293 prep with,incubated
R4297 T5295 T5296 nummod 1,μg
R4298 T5296 T5294 pobj μg,with
R4299 T5297 T5296 prep of,μg
R4300 T5298 T5299 det the,antibody
R4301 T5299 T5297 pobj antibody,of
R4302 T5300 T5302 nsubjpass that,preincubated
R4303 T5301 T5302 auxpass was,preincubated
R4304 T5302 T5299 relcl preincubated,antibody
R4305 T5303 T5302 prep with,preincubated
R4306 T5304 T5305 nummod 1,μg
R4307 T5305 T5303 pobj μg,with
R4308 T5306 T5305 prep of,μg
R4309 T5307 T5308 amod purified,peptide
R4310 T5308 T5306 pobj peptide,of
R4311 T5309 T5302 prep for,preincubated
R4312 T5310 T5311 nummod 1,hr
R4313 T5311 T5309 pobj hr,for
R4314 T5312 T5311 prep at,hr
R4315 T5313 T5314 compound room,temperature
R4316 T5314 T5312 pobj temperature,at
R4317 T5315 T5302 cc or,preincubated
R4318 T5316 T5302 conj incubated,preincubated
R4319 T5317 T5316 prep with,incubated
R4320 T5318 T5319 amod pre-immune,serum
R4321 T5319 T5317 pobj serum,with
R4322 T5320 T5321 advmod instead,of
R4323 T5321 T5319 cc of,serum
R4324 T5322 T5324 det the,antibody
R4325 T5323 T5324 amod primary,antibody
R4326 T5324 T5319 conj antibody,serum
R4327 T5325 T5293 punct .,incubated
R4328 T5326 T5332 prep For,incubated
R4329 T5327 T5328 amod negative,controls
R4330 T5328 T5326 pobj controls,For
R4331 T5329 T5332 punct ",",incubated
R4332 T5330 T5332 nsubjpass tissues,incubated
R4333 T5331 T5332 auxpass were,incubated
R4334 T5332 T5332 ROOT incubated,incubated
R4335 T5333 T5332 prep with,incubated
R4336 T5334 T5335 amod pre-immune,serum
R4337 T5335 T5333 pobj serum,with
R4338 T5336 T5337 advmod instead,of
R4339 T5337 T5335 cc of,serum
R4340 T5338 T5339 amod primary,antibody
R4341 T5339 T5335 conj antibody,serum
R4342 T5340 T5332 punct .,incubated