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PMC:3279418 / 27163-28081 JSONTXT 12 Projects

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Id Subject Object Predicate Lexical cue
T9969 0-10 NNS denotes Constructs
T9970 11-14 CC denotes and
T9971 15-27 NN denotes transfection
T9972 28-31 DT denotes The
T9973 32-45 JJ denotes complementary
T9974 46-49 NN denotes DNA
T9975 50-51 -LRB- denotes (
T9976 51-55 NNP denotes cDNA
T9977 55-56 -RRB- denotes )
T9978 57-59 IN denotes of
T9979 60-63 DT denotes the
T9980 64-69 NN denotes mouse
T9981 70-77 NNP denotes Sharpin
T9982 78-82 NN denotes gene
T9983 83-86 VBD denotes was
T9984 87-93 VBN denotes cloned
T9985 94-97 CC denotes and
T9986 98-107 VBN denotes amplified
T9987 108-112 IN denotes from
T9988 113-119 NNP denotes RAW264
T9989 119-121 CD denotes .7
T9990 122-125 NNP denotes RNA
T9991 126-134 NNS denotes extracts
T9992 134-135 . denotes .
T9993 136-139 DT denotes The
T9994 140-146 NN denotes primer
T9995 147-156 NNS denotes sequences
T9996 157-161 VBD denotes were
T9997 162-169 RB denotes forward
T9998 169-170 , denotes ,
T9999 171-172 CD denotes 5
T10000 172-173 SYM denotes ′
T10001 173-174 : denotes -
T10002 174-176 NNP denotes CC
T10003 177-180 NNP denotes ATG
T10004 181-184 NNP denotes GCG
T10005 185-188 NNP denotes ATG
T10006 189-192 NNP denotes TCG
T10007 193-196 NNP denotes CCG
T10008 197-200 NNP denotes CCC
T10009 201-204 NNP denotes GCC
T10010 205-208 NNP denotes GGC
T10011 209-212 NNP denotes GGT
T10012 212-213 : denotes ;
T10013 214-221 VB denotes reverse
T10014 221-222 , denotes ,
T10015 223-224 CD denotes 5
T10016 224-225 SYM denotes ′
T10017 225-226 : denotes -
T10018 227-230 NNP denotes AAG
T10019 231-234 NNP denotes CTT
T10020 235-238 NNP denotes CTA
T10021 239-242 NNP denotes GGT
T10022 243-246 NNP denotes GGA
T10023 247-250 NNP denotes AGC
T10024 251-254 NNP denotes TGC
T10025 255-258 NNP denotes AGC
T10026 259-262 NNP denotes AAG
T10027 263-264 NNP denotes A
T10028 264-265 . denotes .
T10029 266-269 DT denotes The
T10030 270-277 NNP denotes Sharpin
T10031 278-282 NNP denotes cDNA
T10032 283-286 VBD denotes was
T10033 287-293 VBN denotes cloned
T10034 294-298 IN denotes into
T10035 299-309 NN denotes expression
T10036 310-316 NN denotes vector
T10037 317-328 NN denotes pFLAG-CMV-2
T10038 328-329 . denotes .
T10039 330-336 NNP denotes Murine
T10040 337-348 NNS denotes fibroblasts
T10041 349-352 CC denotes and
T10042 353-364 NNS denotes macrophages
T10043 365-369 VBD denotes were
T10044 370-374 VBD denotes were
T10045 375-379 VBN denotes used
T10046 380-382 TO denotes to
T10047 383-390 VB denotes express
T10048 391-402 JJ denotes recombinant
T10049 403-410 NNP denotes SHARPIN
T10050 411-418 NN denotes protein
T10051 418-419 . denotes .
T10052 420-425 NNS denotes Cells
T10053 426-427 -LRB- denotes (
T10054 427-428 CD denotes 2
T10055 428-429 CD denotes ×
T10056 429-432 CD denotes 104
T10057 432-433 -RRB- denotes )
T10058 434-438 VBD denotes were
T10059 439-445 VBN denotes seeded
T10060 446-448 IN denotes in
T10061 449-456 JJ denotes 96-well
T10062 457-464 VBN denotes treated
T10063 465-471 VBZ denotes plates
T10064 472-475 DT denotes the
T10065 476-479 NN denotes day
T10066 480-486 IN denotes before
T10067 487-499 NN denotes transfection
T10068 499-500 . denotes .
T10069 501-506 IN denotes After
T10070 507-516 JJ denotes overnight
T10071 517-527 NN denotes incubation
T10072 527-528 , denotes ,
T10073 529-532 CD denotes 200
T10074 533-535 NN denotes ng
T10075 536-549 NN denotes pFLAG-SHARPIN
T10076 550-558 NNS denotes plasmids
T10077 559-563 VBD denotes were
T10078 564-575 VBN denotes transfected
T10079 576-580 IN denotes with
T10080 581-584 CD denotes 0.5
T10081 585-587 NN denotes ul
T10082 588-601 NNP denotes Lipofectamine
T10083 602-606 CD denotes 2000
T10084 606-607 . denotes .
T10085 608-610 CD denotes 24
T10086 611-616 NNS denotes hours
T10087 617-622 RB denotes later
T10088 622-623 , denotes ,
T10089 624-629 NNS denotes cells
T10090 630-634 VBD denotes were
T10091 635-644 VBN denotes incubated
T10092 645-649 IN denotes with
T10093 650-655 JJ denotes fresh
T10094 656-663 NN denotes culture
T10095 664-670 NN denotes medium
T10096 670-671 . denotes .
T10097 672-677 IN denotes After
T10098 678-685 DT denotes another
T10099 686-688 CD denotes 24
T10100 689-694 NNS denotes hours
T10101 694-695 , denotes ,
T10102 696-701 NNS denotes cells
T10103 702-706 VBD denotes were
T10104 707-712 VBN denotes lysed
T10105 713-715 TO denotes to
T10106 716-723 VB denotes confirm
T10107 724-727 DT denotes the
T10108 728-740 NNP denotes FLAG-SHARPIN
T10109 741-751 NN denotes expression
T10110 752-754 IN denotes by
T10111 755-766 NNS denotes immunoblots
T10112 767-771 IN denotes with
T10113 772-781 JJ denotes anti-FLAG
T10114 781-782 . denotes .
T10115 783-788 NNS denotes Cells
T10116 789-793 IN denotes with
T10117 794-796 DT denotes no
T10118 797-809 NN denotes transfection
T10119 810-813 CC denotes and
T10120 814-825 VBN denotes transfected
T10121 826-830 IN denotes with
T10122 831-836 JJ denotes empty
T10123 837-843 NN denotes vector
T10124 844-855 NN denotes pFLAG-CMV-2
T10125 856-860 VBD denotes were
T10126 861-865 VBN denotes used
T10127 866-868 IN denotes as
T10128 869-877 JJ denotes negative
T10129 878-885 NN denotes control
T10130 886-888 IN denotes in
T10131 889-892 DT denotes all
T10132 893-905 NN denotes transfection
T10133 906-917 NNS denotes experiments
T10134 917-918 . denotes .
R8184 T9969 T9969 ROOT Constructs,Constructs
R8185 T9970 T9969 cc and,Constructs
R8186 T9971 T9969 conj transfection,Constructs
R8187 T9972 T9974 det The,DNA
R8188 T9973 T9974 amod complementary,DNA
R8189 T9974 T9984 nsubjpass DNA,cloned
R8190 T9975 T9974 punct (,DNA
R8191 T9976 T9974 appos cDNA,DNA
R8192 T9977 T9974 punct ),DNA
R8193 T9978 T9974 prep of,DNA
R8194 T9979 T9982 det the,gene
R8195 T9980 T9982 compound mouse,gene
R8196 T9981 T9982 compound Sharpin,gene
R8197 T9982 T9978 pobj gene,of
R8198 T9983 T9984 auxpass was,cloned
R8199 T9984 T9984 ROOT cloned,cloned
R8200 T9985 T9984 cc and,cloned
R8201 T9986 T9984 conj amplified,cloned
R8202 T9987 T9986 prep from,amplified
R8203 T9988 T9990 compound RAW264,RNA
R8204 T9989 T9990 compound .7,RNA
R8205 T9990 T9991 compound RNA,extracts
R8206 T9991 T9987 pobj extracts,from
R8207 T9992 T9984 punct .,cloned
R8208 T9993 T9995 det The,sequences
R8209 T9994 T9995 compound primer,sequences
R8210 T9995 T9996 nsubj sequences,were
R8211 T9996 T10027 ccomp were,A
R8212 T9997 T9996 advmod forward,were
R8213 T9998 T9996 punct ",",were
R8214 T9999 T10011 nummod 5,GGT
R8215 T10000 T10002 punct ′,CC
R8216 T10001 T10002 punct -,CC
R8217 T10002 T10004 compound CC,GCG
R8218 T10003 T10004 compound ATG,GCG
R8219 T10004 T10011 compound GCG,GGT
R8220 T10005 T10011 compound ATG,GGT
R8221 T10006 T10011 compound TCG,GGT
R8222 T10007 T10011 compound CCG,GGT
R8223 T10008 T10011 compound CCC,GGT
R8224 T10009 T10011 compound GCC,GGT
R8225 T10010 T10011 compound GGC,GGT
R8226 T10011 T9996 attr GGT,were
R8227 T10012 T10027 punct ;,A
R8228 T10013 T10027 advmod reverse,A
R8229 T10014 T10027 punct ",",A
R8230 T10015 T10027 nummod 5,A
R8231 T10016 T10027 punct ′,A
R8232 T10017 T10027 punct -,A
R8233 T10018 T10027 compound AAG,A
R8234 T10019 T10027 compound CTT,A
R8235 T10020 T10027 compound CTA,A
R8236 T10021 T10027 compound GGT,A
R8237 T10022 T10027 compound GGA,A
R8238 T10023 T10027 compound AGC,A
R8239 T10024 T10027 compound TGC,A
R8240 T10025 T10027 compound AGC,A
R8241 T10026 T10027 compound AAG,A
R8242 T10027 T10027 ROOT A,A
R8243 T10028 T10027 punct .,A
R8244 T10029 T10031 det The,cDNA
R8245 T10030 T10031 compound Sharpin,cDNA
R8246 T10031 T10033 nsubjpass cDNA,cloned
R8247 T10032 T10033 auxpass was,cloned
R8248 T10033 T10033 ROOT cloned,cloned
R8249 T10034 T10033 prep into,cloned
R8250 T10035 T10036 compound expression,vector
R8251 T10036 T10037 compound vector,pFLAG-CMV-2
R8252 T10037 T10034 pobj pFLAG-CMV-2,into
R8253 T10038 T10033 punct .,cloned
R8254 T10039 T10040 compound Murine,fibroblasts
R8255 T10040 T10045 nsubjpass fibroblasts,used
R8256 T10041 T10040 cc and,fibroblasts
R8257 T10042 T10040 conj macrophages,fibroblasts
R8258 T10043 T10045 auxpass were,used
R8259 T10044 T10045 auxpass were,used
R8260 T10045 T10045 ROOT used,used
R8261 T10046 T10047 aux to,express
R8262 T10047 T10045 xcomp express,used
R8263 T10048 T10050 compound recombinant,protein
R8264 T10049 T10050 compound SHARPIN,protein
R8265 T10050 T10047 dobj protein,express
R8266 T10051 T10045 punct .,used
R8267 T10052 T10059 nsubjpass Cells,seeded
R8268 T10053 T10052 punct (,Cells
R8269 T10054 T10056 nummod 2,104
R8270 T10055 T10056 nummod ×,104
R8271 T10056 T10052 appos 104,Cells
R8272 T10057 T10052 punct ),Cells
R8273 T10058 T10059 auxpass were,seeded
R8274 T10059 T10059 ROOT seeded,seeded
R8275 T10060 T10059 prep in,seeded
R8276 T10061 T10062 npadvmod 96-well,treated
R8277 T10062 T10063 amod treated,plates
R8278 T10063 T10060 pobj plates,in
R8279 T10064 T10065 det the,day
R8280 T10065 T10063 npadvmod day,plates
R8281 T10066 T10059 prep before,seeded
R8282 T10067 T10066 pobj transfection,before
R8283 T10068 T10059 punct .,seeded
R8284 T10069 T10078 prep After,transfected
R8285 T10070 T10071 amod overnight,incubation
R8286 T10071 T10069 pobj incubation,After
R8287 T10072 T10078 punct ",",transfected
R8288 T10073 T10076 nummod 200,plasmids
R8289 T10074 T10076 compound ng,plasmids
R8290 T10075 T10076 compound pFLAG-SHARPIN,plasmids
R8291 T10076 T10078 nsubjpass plasmids,transfected
R8292 T10077 T10078 auxpass were,transfected
R8293 T10078 T10078 ROOT transfected,transfected
R8294 T10079 T10078 prep with,transfected
R8295 T10080 T10081 nummod 0.5,ul
R8296 T10081 T10082 compound ul,Lipofectamine
R8297 T10082 T10079 pobj Lipofectamine,with
R8298 T10083 T10082 nummod 2000,Lipofectamine
R8299 T10084 T10078 punct .,transfected
R8300 T10085 T10086 nummod 24,hours
R8301 T10086 T10087 npadvmod hours,later
R8302 T10087 T10091 advmod later,incubated
R8303 T10088 T10091 punct ",",incubated
R8304 T10089 T10091 nsubjpass cells,incubated
R8305 T10090 T10091 auxpass were,incubated
R8306 T10091 T10091 ROOT incubated,incubated
R8307 T10092 T10091 prep with,incubated
R8308 T10093 T10095 amod fresh,medium
R8309 T10094 T10095 compound culture,medium
R8310 T10095 T10092 pobj medium,with
R8311 T10096 T10091 punct .,incubated
R8312 T10097 T10104 prep After,lysed
R8313 T10098 T10100 det another,hours
R8314 T10099 T10100 nummod 24,hours
R8315 T10100 T10097 pobj hours,After
R8316 T10101 T10104 punct ",",lysed
R8317 T10102 T10104 nsubjpass cells,lysed
R8318 T10103 T10104 auxpass were,lysed
R8319 T10104 T10104 ROOT lysed,lysed
R8320 T10105 T10106 aux to,confirm
R8321 T10106 T10104 advcl confirm,lysed
R8322 T10107 T10109 det the,expression
R8323 T10108 T10109 compound FLAG-SHARPIN,expression
R8324 T10109 T10106 dobj expression,confirm
R8325 T10110 T10109 prep by,expression
R8326 T10111 T10110 pobj immunoblots,by
R8327 T10112 T10111 prep with,immunoblots
R8328 T10113 T10112 pobj anti-FLAG,with
R8329 T10114 T10104 punct .,lysed
R8330 T10115 T10120 nsubj Cells,transfected
R8331 T10116 T10115 prep with,Cells
R8332 T10117 T10118 det no,transfection
R8333 T10118 T10116 pobj transfection,with
R8334 T10119 T10115 cc and,Cells
R8335 T10120 T10126 nsubjpass transfected,used
R8336 T10121 T10120 prep with,transfected
R8337 T10122 T10124 amod empty,pFLAG-CMV-2
R8338 T10123 T10124 compound vector,pFLAG-CMV-2
R8339 T10124 T10121 pobj pFLAG-CMV-2,with
R8340 T10125 T10126 auxpass were,used
R8341 T10126 T10126 ROOT used,used
R8342 T10127 T10126 prep as,used
R8343 T10128 T10129 amod negative,control
R8344 T10129 T10127 pobj control,as
R8345 T10130 T10126 prep in,used
R8346 T10131 T10133 det all,experiments
R8347 T10132 T10133 compound transfection,experiments
R8348 T10133 T10130 pobj experiments,in
R8349 T10134 T10126 punct .,used