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PMC:3245220 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T58 0-5 NNP denotes M-CSF
T59 6-13 NNP denotes Induces
T60 14-22 NNP denotes Monocyte
T61 23-31 NNP denotes Survival
T62 32-34 IN denotes by
T63 35-45 NNP denotes Activating
T64 46-51 NNP denotes NF-κB
T14519 46-51 JJ denotes NF-κB
T65 52-55 CD denotes p65
T66 56-71 NNP denotes Phosphorylation
T67 72-74 IN denotes at
T68 75-81 NNP denotes Ser276
T69 82-85 IN denotes via
T70 86-93 NNP denotes Protein
T71 94-100 NNP denotes Kinase
T72 101-102 NNP denotes C
T73 134-144 NNP denotes Macrophage
T74 145-163 JJ denotes colony-stimulating
T75 164-170 NN denotes factor
T76 171-172 -LRB- denotes (
T77 172-177 NNP denotes M-CSF
T78 177-178 -RRB- denotes )
T79 179-187 VBZ denotes promotes
T80 188-199 JJ denotes mononuclear
T81 200-209 JJ denotes phagocyte
T82 210-218 NN denotes survival
T83 219-222 CC denotes and
T84 223-236 NN denotes proliferation
T85 236-237 . denotes .
T86 238-241 DT denotes The
T87 242-255 NN denotes transcription
T14520 242-454 JJ denotes transcription factor Nuclear Factor-kappaB (NF-κB) is a key regulator of genes involved in M-CSF-induced mononuclear phagocyte survival and this study focused at identifying the mechanism of NF-κB transcriptional
T88 256-262 NN denotes factor
T89 263-270 NNP denotes Nuclear
T90 271-284 NNP denotes Factor-kappaB
T91 285-286 -LRB- denotes (
T92 286-291 NNP denotes NF-κB
T93 291-292 -RRB- denotes )
T94 293-295 VBZ denotes is
T95 296-297 DT denotes a
T96 298-301 JJ denotes key
T97 302-311 NN denotes regulator
T98 312-314 IN denotes of
T99 315-320 NNS denotes genes
T100 321-329 VBN denotes involved
T101 330-332 IN denotes in
T102 333-346 JJ denotes M-CSF-induced
T103 347-358 NN denotes mononuclear
T104 359-368 NN denotes phagocyte
T105 369-377 NN denotes survival
T106 378-381 CC denotes and
T107 382-386 DT denotes this
T108 387-392 NN denotes study
T109 393-400 VBD denotes focused
T110 401-403 IN denotes at
T111 404-415 VBG denotes identifying
T112 416-419 DT denotes the
T113 420-429 NN denotes mechanism
T114 430-432 IN denotes of
T115 433-438 JJ denotes NF-κB
T116 439-454 JJ denotes transcriptional
T117 455-465 NN denotes activation
T14521 455-540 NN denotes activation. Here, we demonstrate that M-CSF stimulated NF-κB transcriptional activity
T118 465-466 . denotes .
T119 467-471 RB denotes Here
T120 471-472 , denotes ,
T121 473-475 PRP denotes we
T122 476-487 VBP denotes demonstrate
T123 488-492 IN denotes that
T124 493-498 NNP denotes M-CSF
T125 499-509 VBD denotes stimulated
T126 510-515 NNP denotes NF-κB
T127 516-531 JJ denotes transcriptional
T128 532-540 NN denotes activity
T129 541-543 IN denotes in
T14522 541-1876 VBZ denotes in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is
T130 544-549 JJ denotes human
T131 550-566 JJ denotes monocyte-derived
T132 567-578 NNS denotes macrophages
T133 579-580 -LRB- denotes (
T134 580-584 NNS denotes MDMs
T135 584-585 -RRB- denotes )
T136 586-589 CC denotes and
T137 590-593 DT denotes the
T138 594-600 NN denotes murine
T139 601-611 NN denotes macrophage
T140 612-616 NN denotes cell
T141 617-621 NN denotes line
T142 622-625 NNP denotes RAW
T143 626-631 CD denotes 264.7
T144 631-632 . denotes .
T145 633-636 DT denotes The
T146 637-644 JJ denotes general
T147 645-652 NN denotes protein
T148 653-659 NN denotes kinase
T149 660-661 NNP denotes C
T150 662-663 -LRB- denotes (
T151 663-666 NNP denotes PKC
T152 666-667 -RRB- denotes )
T153 668-677 NN denotes inhibitor
T154 678-688 NN denotes Ro-31-8220
T155 688-689 , denotes ,
T156 690-693 DT denotes the
T157 694-706 JJ denotes conventional
T158 707-711 NNP denotes PKCα
T159 711-712 NN denotes /
T160 712-713 NN denotes β
T161 714-723 NN denotes inhibitor
T162 724-731 NNP denotes Gö-6976
T163 731-732 , denotes ,
T164 733-747 NN denotes overexpression
T165 748-750 IN denotes of
T166 751-759 JJ denotes dominant
T167 760-768 JJ denotes negative
T168 769-773 NNP denotes PKCα
T169 774-784 NNS denotes constructs
T170 785-788 CC denotes and
T171 789-793 NNP denotes PKCα
T172 794-799 NNP denotes siRNA
T173 800-807 VBD denotes reduced
T174 808-813 JJ denotes NF-κB
T175 814-822 NN denotes activity
T176 823-825 IN denotes in
T177 826-834 NN denotes response
T178 835-837 TO denotes to
T179 838-843 NNP denotes M-CSF
T180 843-844 . denotes .
T181 845-858 RB denotes Interestingly
T182 858-859 , denotes ,
T183 860-870 JJ denotes Ro-31-8220
T184 871-878 JJ denotes reduced
T185 879-885 NNP denotes Ser276
T186 886-901 NN denotes phosphorylation
T187 902-904 IN denotes of
T188 905-913 NN denotes NF-κBp65
T189 914-921 VBG denotes leading
T190 922-924 TO denotes to
T191 925-934 VBN denotes decreased
T192 935-948 JJ denotes M-CSF-induced
T193 949-957 NN denotes monocyte
T194 958-966 NN denotes survival
T195 966-967 . denotes .
T196 968-970 IN denotes In
T197 971-975 DT denotes this
T198 976-982 NN denotes report
T199 982-983 , denotes ,
T200 984-986 PRP denotes we
T201 987-995 VBP denotes identify
T202 996-1008 JJ denotes conventional
T203 1009-1013 NNS denotes PKCs
T204 1013-1014 , denotes ,
T205 1015-1024 VBG denotes including
T206 1025-1029 NNP denotes PKCα
T207 1030-1032 IN denotes as
T208 1033-1042 JJ denotes important
T209 1043-1051 JJ denotes upstream
T210 1052-1059 NNS denotes kinases
T211 1060-1063 IN denotes for
T212 1064-1077 JJ denotes M-CSF-induced
T213 1078-1083 NN denotes NF-κB
T214 1084-1099 JJ denotes transcriptional
T215 1100-1110 NN denotes activation
T216 1110-1111 , denotes ,
T217 1112-1127 JJ denotes NF-κB-regulated
T218 1128-1132 NN denotes gene
T219 1133-1143 NN denotes expression
T220 1143-1144 , denotes ,
T221 1145-1150 NNP denotes NF-κB
T222 1151-1154 CD denotes p65
T223 1155-1161 NNP denotes Ser276
T224 1162-1177 NN denotes phosphorylation
T225 1177-1178 , denotes ,
T226 1179-1182 CC denotes and
T227 1183-1193 NN denotes macrophage
T228 1194-1202 NN denotes survival
T229 1202-1203 . denotes .
T230 1204-1210 RB denotes Lastly
T231 1210-1211 , denotes ,
T232 1212-1214 PRP denotes we
T233 1215-1219 VBP denotes find
T234 1220-1224 IN denotes that
T235 1225-1230 NNP denotes NF-κB
T236 1231-1234 CD denotes p65
T237 1235-1241 NNP denotes Ser276
T238 1242-1247 VBZ denotes plays
T239 1248-1250 DT denotes an
T240 1251-1260 JJ denotes important
T241 1261-1265 NN denotes role
T242 1266-1268 IN denotes in
T243 1269-1274 JJ denotes basal
T244 1275-1278 CC denotes and
T245 1279-1295 JJ denotes M-CSF-stimulated
T246 1296-1301 NN denotes NF-κB
T247 1302-1312 NN denotes activation
T248 1313-1315 IN denotes in
T249 1316-1321 JJ denotes human
T250 1322-1333 NN denotes mononuclear
T251 1334-1344 NNS denotes phagocytes
T252 1344-1345 . denotes .
T1018 1360-1369 NNS denotes Monocytes
T1019 1370-1373 VBP denotes are
T1020 1374-1382 VBN denotes produced
T1021 1383-1385 IN denotes in
T1022 1386-1389 DT denotes the
T1023 1390-1394 NN denotes bone
T1024 1395-1401 NN denotes marrow
T1025 1402-1405 CC denotes and
T1026 1406-1415 VBP denotes circulate
T1027 1416-1418 IN denotes in
T1028 1419-1424 NN denotes blood
T1029 1425-1428 IN denotes for
T1030 1429-1431 CD denotes 24
T1031 1432-1434 CD denotes 48
T1032 1435-1440 NNS denotes hours
T1033 1441-1442 NNP denotes [
T1034 1442-1443 CD denotes 1
T1035 1443-1444 NNP denotes ]
T1036 1444-1445 . denotes .
T1037 1446-1448 IN denotes In
T1038 1449-1452 DT denotes the
T1039 1453-1460 NN denotes absence
T1040 1461-1463 IN denotes of
T1041 1464-1469 NN denotes serum
T1042 1469-1470 , denotes ,
T1043 1471-1480 NNS denotes monocytes
T1044 1481-1484 VBP denotes die
T1045 1485-1488 IN denotes via
T1046 1489-1498 NN denotes apoptosis
T1047 1499-1500 NN denotes [
T1048 1500-1501 CD denotes 1
T1049 1501-1502 NNP denotes ]
T1050 1502-1503 , denotes ,
T1051 1504-1505 NNP denotes [
T1052 1505-1506 CD denotes 2
T1053 1506-1507 NNP denotes ]
T1054 1507-1508 . denotes .
T1055 1509-1519 NNP denotes Macrophage
T1056 1520-1538 JJ denotes colony-stimulating
T1057 1539-1545 NN denotes factor
T1058 1546-1547 -LRB- denotes (
T1059 1547-1552 NNP denotes M-CSF
T1060 1552-1553 -RRB- denotes )
T1061 1554-1564 VBZ denotes stimulates
T1062 1565-1576 JJ denotes mononuclear
T1063 1577-1586 JJ denotes phagocyte
T1064 1587-1595 NN denotes survival
T1065 1596-1599 CC denotes and
T1066 1600-1615 NN denotes differentiation
T1067 1616-1617 NN denotes [
T1068 1617-1618 CD denotes 3
T1069 1618-1619 NNP denotes ]
T1070 1619-1620 . denotes .
T1071 1621-1632 RB denotes Importantly
T1072 1632-1633 , denotes ,
T1073 1634-1648 JJ denotes M-CSF-mediated
T1074 1649-1653 NN denotes cell
T1075 1654-1662 NN denotes survival
T1076 1663-1666 CC denotes and
T1077 1667-1677 NN denotes activation
T1078 1678-1680 VBZ denotes is
T1079 1681-1691 VBN denotes associated
T1080 1692-1696 IN denotes with
T1081 1697-1698 DT denotes a
T1082 1699-1706 NN denotes variety
T1083 1707-1709 IN denotes of
T1084 1710-1715 JJ denotes human
T1085 1716-1724 NNS denotes diseases
T1086 1724-1725 , denotes ,
T1087 1726-1735 VBG denotes including
T1088 1736-1751 NN denotes atherosclerosis
T1089 1751-1752 , denotes ,
T1090 1753-1763 NN denotes transplant
T1091 1764-1772 JJ denotes vascular
T1092 1773-1782 NN denotes sclerosis
T1093 1783-1786 CC denotes and
T1094 1787-1793 NN denotes breast
T1095 1794-1800 NN denotes cancer
T1096 1801-1811 NN denotes metastasis
T1097 1812-1813 NNP denotes [
T1098 1813-1814 CD denotes 4
T1099 1814-1815 NNP denotes ]
T1100 1815-1816 , denotes ,
T1101 1817-1818 NNP denotes [
T1102 1818-1819 CD denotes 5
T1103 1819-1820 NNP denotes ]
T1104 1820-1821 , denotes ,
T1105 1822-1823 NNP denotes [
T1106 1823-1824 CD denotes 6
T1107 1824-1825 NNP denotes ]
T1108 1825-1826 . denotes .
T1109 1827-1829 PRP denotes We
T1110 1830-1840 RB denotes previously
T1111 1841-1851 VBD denotes identified
T1112 1852-1856 IN denotes that
T1113 1857-1862 JJ denotes NF-κB
T1114 1863-1873 NN denotes activation
T1115 1874-1876 VBZ denotes is
T1116 1877-1886 JJ denotes important
T14523 1877-1886 JJ denotes important
T1117 1887-1889 IN denotes in
T14524 1887-1889 IN denotes in
T1118 1890-1903 JJ denotes M-CSF-induced
T1119 1904-1912 NN denotes monocyte
T1120 1913-1921 NN denotes survival
T1121 1922-1923 NN denotes [
T1122 1923-1924 CD denotes 7
T1123 1924-1925 NNP denotes ]
T1124 1925-1926 . denotes .
T1125 1927-1929 IN denotes In
T1126 1930-1938 NN denotes addition
T1127 1939-1941 TO denotes to
T1128 1942-1945 PRP$ denotes its
T1129 1946-1950 NN denotes role
T1130 1951-1953 IN denotes in
T1131 1954-1965 NN denotes mononuclear
T1132 1966-1975 JJ denotes phagocyte
T1133 1976-1984 NN denotes survival
T1134 1984-1985 , denotes ,
T1135 1986-1989 DT denotes the
T1136 1990-2003 NN denotes transcription
T1137 2004-2010 NN denotes factor
T1138 2011-2016 NNP denotes NF-κB
T1139 2017-2026 VBZ denotes regulates
T1140 2027-2035 JJ denotes numerous
T1141 2036-2041 NNS denotes genes
T1142 2042-2046 WDT denotes that
T1143 2047-2051 VBP denotes play
T1144 2052-2061 JJ denotes important
T1145 2062-2067 NNS denotes roles
T1146 2068-2070 IN denotes in
T1147 2071-2079 JJ denotes cellular
T1148 2080-2089 VBG denotes signaling
T1149 2089-2090 , denotes ,
T1150 2091-2097 NN denotes stress
T1151 2098-2106 NN denotes response
T1152 2106-2107 , denotes ,
T1153 2108-2112 NN denotes cell
T1154 2113-2119 NN denotes growth
T1155 2119-2120 , denotes ,
T1156 2121-2129 NN denotes survival
T1157 2129-2130 , denotes ,
T1158 2131-2146 NN denotes differentiation
T1159 2147-2150 CC denotes and
T1160 2151-2163 NN denotes inflammation
T1161 2164-2165 NN denotes [
T1162 2165-2166 CD denotes 8
T1163 2166-2167 NNP denotes ]
T1164 2167-2168 . denotes .
T1165 2169-2174 EX denotes There
T1166 2175-2178 VBP denotes are
T1167 2179-2183 CD denotes five
T1168 2184-2191 NNS denotes members
T1169 2192-2194 IN denotes in
T1170 2195-2198 DT denotes the
T1171 2199-2204 NNP denotes NF-κB
T1172 2205-2211 NN denotes family
T1173 2211-2212 : denotes :
T1174 2213-2221 NNS denotes RelA/p65
T1175 2221-2222 , denotes ,
T1176 2223-2226 CD denotes p50
T1177 2226-2227 , denotes ,
T1178 2228-2231 CD denotes p52
T1179 2231-2232 , denotes ,
T1180 2233-2238 NNP denotes c-Rel
T1181 2239-2242 CC denotes and
T1182 2243-2247 NNP denotes RelB
T1183 2247-2248 . denotes .
T1184 2249-2252 DT denotes The
T1185 2253-2257 RBS denotes most
T1186 2258-2264 JJ denotes common
T1187 2265-2275 NN denotes activating
T1188 2276-2283 NN denotes complex
T1189 2284-2286 VBZ denotes is
T1190 2287-2290 DT denotes the
T1191 2291-2298 CD denotes p50/p65
T1192 2299-2310 NN denotes heterodimer
T1193 2310-2311 , denotes ,
T1194 2312-2318 VBN denotes driven
T1195 2319-2321 IN denotes by
T1196 2322-2325 DT denotes the
T1197 2326-2336 NN denotes activation
T1198 2337-2343 NN denotes domain
T1199 2344-2346 IN denotes in
T1200 2347-2350 DT denotes the
T1201 2351-2356 NNP denotes NF-κB
T1202 2357-2360 CD denotes p65
T1203 2361-2368 NN denotes subunit
T1204 2368-2369 . denotes .
T1205 2370-2375 NNP denotes NF-κB
T1206 2376-2379 CD denotes p65
T1207 2380-2389 VBZ denotes regulates
T1208 2390-2399 JJ denotes important
T1209 2400-2413 JJ denotes developmental
T1210 2414-2423 NNS denotes processes
T1211 2424-2425 VBP denotes [
T1212 2425-2426 CD denotes 9
T1213 2426-2427 NNP denotes ]
T1214 2427-2428 , denotes ,
T1215 2429-2430 NNP denotes [
T1216 2430-2432 CD denotes 10
T1217 2432-2433 NNP denotes ]
T1218 2433-2434 . denotes .
T1219 2435-2439 NNP denotes Mice
T1220 2440-2447 VBG denotes lacking
T1221 2448-2453 NNP denotes NF-κB
T1222 2454-2457 CD denotes p65
T1223 2458-2461 NN denotes die
T1224 2462-2464 IN denotes in
T1225 2465-2470 NN denotes utero
T1226 2471-2474 CC denotes and
T1227 2475-2479 VBP denotes have
T1228 2480-2489 JJ denotes extensive
T1229 2490-2495 NN denotes liver
T1230 2496-2502 NN denotes damage
T1231 2503-2506 IN denotes via
T1232 2507-2515 JJ denotes enhanced
T1233 2516-2525 NN denotes apoptosis
T1234 2526-2527 NN denotes [
T1235 2527-2528 CD denotes 9
T1236 2528-2529 NNP denotes ]
T1237 2529-2530 . denotes .
T1238 2531-2540 JJ denotes Embryonic
T1239 2541-2552 NNS denotes macrophages
T1240 2553-2557 IN denotes from
T1241 2558-2563 NNP denotes NF-κB
T1242 2564-2567 CD denotes p65
T1243 2568-2572 NN denotes null
T1244 2573-2577 NNS denotes mice
T1245 2578-2581 VBP denotes are
T1246 2582-2593 JJ denotes susceptible
T1247 2594-2596 TO denotes to
T1248 2597-2609 JJ denotes TNFα-induced
T1249 2610-2619 NN denotes apoptosis
T1250 2620-2625 WDT denotes which
T1251 2626-2628 VBZ denotes is
T1252 2629-2636 VBN denotes rescued
T1253 2637-2639 IN denotes by
T1254 2640-2654 VBG denotes overexpressing
T1255 2655-2658 DT denotes the
T1256 2659-2664 NNP denotes NF-κB
T1257 2665-2668 CD denotes p65
T1258 2669-2676 VBN denotes subunit
T1259 2677-2678 NNP denotes [
T1260 2678-2680 CD denotes 10
T1261 2680-2681 NNP denotes ]
T1262 2681-2682 . denotes .
T1263 2683-2691 RB denotes Moreover
T1264 2691-2692 , denotes ,
T1265 2693-2703 VBG denotes inhibiting
T1266 2704-2709 JJ denotes NF-κB
T1267 2710-2717 VBZ denotes induces
T1268 2718-2722 NN denotes cell
T1269 2723-2728 NN denotes death
T1270 2729-2731 IN denotes in
T1271 2732-2736 JJ denotes many
T1272 2737-2741 NN denotes cell
T1273 2742-2747 NNS denotes types
T1274 2748-2751 CC denotes and
T1275 2752-2772 JJ denotes cytokine-independent
T1276 2773-2781 NN denotes survival
T1277 2782-2784 VBZ denotes is
T1278 2785-2793 VBN denotes mediated
T1279 2794-2796 IN denotes by
T1280 2797-2811 RB denotes constitutively
T1281 2812-2818 JJ denotes active
T1282 2819-2824 NN denotes NF-κB
T1283 2825-2827 IN denotes in
T1284 2828-2834 JJ denotes murine
T1285 2835-2846 NNS denotes macrophages
T1286 2847-2848 NNP denotes [
T1287 2848-2850 CD denotes 11
T1288 2850-2851 NNP denotes ]
T1289 2851-2852 . denotes .
T1290 2853-2855 IN denotes In
T1291 2856-2865 NNS denotes monocytes
T1292 2866-2869 CC denotes and
T1293 2870-2881 NNS denotes macrophages
T1294 2881-2882 , denotes ,
T1295 2883-2888 NN denotes NF-κB
T1296 2889-2891 VBZ denotes is
T1297 2892-2894 DT denotes an
T1298 2895-2904 JJ denotes important
T1299 2905-2920 JJ denotes transcriptional
T1300 2921-2927 NN denotes factor
T1301 2928-2931 IN denotes for
T1302 2932-2942 NN denotes expression
T1303 2943-2945 IN denotes of
T1304 2946-2955 NNS denotes cytokines
T1305 2956-2959 CC denotes and
T1306 2960-2964 NN denotes cell
T1307 2965-2972 NN denotes surface
T1308 2973-2982 NNS denotes receptors
T1309 2983-2984 NNP denotes [
T1310 2984-2986 CD denotes 12
T1311 2986-2987 NNP denotes ]
T1312 2987-2988 . denotes .
T1313 2989-2996 RB denotes However
T1314 2996-2997 , denotes ,
T1315 2998-3004 IN denotes unlike
T1316 3005-3012 VBG denotes resting
T1317 3013-3020 NNS denotes T-cells
T1318 3020-3021 , denotes ,
T1319 3022-3027 NN denotes NF-κB
T1320 3028-3030 VBZ denotes is
T1321 3031-3045 RB denotes constitutively
T1322 3046-3053 JJ denotes present
T1323 3054-3056 IN denotes in
T1324 3057-3060 DT denotes the
T1325 3061-3067 NN denotes nuclei
T1326 3068-3070 IN denotes of
T1327 3071-3078 JJ denotes primary
T1328 3079-3088 NNS denotes monocytes
T1329 3089-3092 CC denotes and
T1330 3093-3102 JJ denotes monocytic
T1331 3103-3107 NN denotes cell
T1332 3108-3113 NNS denotes lines
T1333 3114-3116 IN denotes in
T1334 3117-3120 DT denotes the
T1335 3121-3128 NN denotes absence
T1336 3129-3131 IN denotes of
T1337 3132-3141 JJ denotes exogenous
T1338 3142-3149 NNS denotes stimuli
T1339 3150-3152 IN denotes as
T1340 3153-3165 VBN denotes demonstrated
T1341 3166-3168 IN denotes by
T1342 3169-3177 NN denotes mobility
T1343 3178-3183 NN denotes shift
T1344 3184-3192 NN denotes analysis
T1345 3193-3194 NNP denotes [
T1346 3194-3196 CD denotes 13
T1347 3196-3197 NNP denotes ]
T1348 3197-3198 . denotes .
T1349 3199-3208 RB denotes Similarly
T1350 3208-3209 , denotes ,
T1351 3210-3224 RB denotes constitutively
T1352 3225-3231 JJ denotes active
T1353 3232-3237 NN denotes NF-κB
T1354 3238-3241 VBD denotes was
T1355 3242-3250 VBN denotes observed
T1356 3251-3253 IN denotes in
T1357 3254-3259 JJ denotes human
T1358 3260-3268 JJ denotes alveolar
T1359 3269-3280 NNS denotes macrophages
T1360 3281-3282 NNP denotes [
T1361 3282-3284 CD denotes 14
T1362 3284-3285 NNP denotes ]
T1363 3285-3286 . denotes .
T1364 3287-3289 IN denotes In
T1365 3290-3293 DT denotes the
T1366 3294-3311 JJ denotes classic/canonical
T1367 3312-3319 NN denotes pathway
T1368 3319-3320 , denotes ,
T1369 3321-3324 DT denotes the
T1370 3325-3330 NNP denotes NF-κB
T1371 3331-3338 CD denotes p50/p65
T1372 3339-3346 NN denotes complex
T1373 3347-3349 VBZ denotes is
T1374 3350-3361 VBN denotes sequestered
T1375 3362-3364 IN denotes in
T1376 3365-3368 DT denotes the
T1377 3369-3376 NN denotes cytosol
T1378 3377-3379 IN denotes by
T1379 3380-3384 NNP denotes IκBα
T1380 3385-3386 NNP denotes [
T1381 3386-3388 CD denotes 15
T1382 3388-3389 NNP denotes ]
T1383 3389-3390 . denotes .
T1384 3391-3395 IN denotes Upon
T1385 3396-3407 NN denotes stimulation
T1386 3408-3410 IN denotes by
T1387 3411-3420 NNS denotes cytokines
T1388 3421-3423 CC denotes or
T1389 3424-3426 NN denotes UV
T1390 3427-3436 NN denotes radiation
T1391 3436-3437 , denotes ,
T1392 3438-3442 NNP denotes IκBα
T1393 3443-3445 VBZ denotes is
T1394 3446-3460 VBN denotes phosphorylated
T1395 3460-3461 , denotes ,
T1396 3462-3475 VBN denotes ubiquitinated
T1397 3475-3476 , denotes ,
T1398 3477-3480 CC denotes and
T1399 3481-3489 JJ denotes degraded
T1400 3489-3490 , denotes ,
T1401 3491-3500 VBG denotes releasing
T1402 3501-3506 NNP denotes NF-κB
T1403 3507-3514 CD denotes p50/p65
T1404 3515-3517 TO denotes to
T1405 3518-3529 VB denotes translocate
T1406 3530-3532 TO denotes to
T1407 3533-3536 DT denotes the
T1408 3537-3544 NN denotes nucleus
T1409 3545-3548 CC denotes and
T1410 3549-3562 NN denotes transactivate
T1411 3563-3569 NN denotes target
T1412 3570-3575 NNS denotes genes
T1413 3575-3576 . denotes .
T1414 3577-3582 IN denotes After
T1415 3583-3586 PRP$ denotes its
T1416 3587-3594 NN denotes release
T1417 3595-3599 IN denotes from
T1418 3600-3604 NNP denotes IκBα
T1419 3604-3605 , denotes ,
T1420 3606-3611 NNP denotes NF-κB
T1421 3612-3615 NNS denotes p65
T1422 3616-3619 MD denotes can
T1423 3620-3627 VB denotes undergo
T1424 3628-3646 JJ denotes post-translational
T1425 3647-3659 NN denotes modification
T1426 3660-3662 TO denotes to
T1427 3663-3671 VB denotes activate
T1428 3672-3676 NN denotes gene
T1429 3677-3690 NN denotes transcription
T1430 3690-3691 . denotes .
T1431 3692-3695 DT denotes The
T1432 3696-3700 NN denotes role
T1433 3701-3703 IN denotes of
T1434 3704-3709 NNP denotes NF-κB
T1435 3710-3713 CD denotes p65
T1436 3714-3729 NN denotes phosphorylation
T1437 3730-3732 IN denotes on
T1438 3733-3738 JJ denotes NF-κB
T1439 3739-3754 JJ denotes transcriptional
T1440 3755-3763 NN denotes activity
T1441 3764-3770 VBZ denotes varies
T1442 3771-3773 IN denotes by
T1443 3774-3782 NN denotes stimulus
T1444 3782-3783 , denotes ,
T1445 3784-3788 NN denotes time
T1446 3789-3791 IN denotes of
T1447 3792-3803 NN denotes stimulation
T1448 3804-3807 CC denotes and
T1449 3808-3812 NN denotes cell
T1450 3813-3817 NN denotes type
T1451 3818-3819 NNP denotes [
T1452 3819-3821 CD denotes 16
T1453 3821-3822 NNP denotes ]
T1454 3822-3823 . denotes .
T1455 3824-3832 JJ denotes Previous
T1456 3833-3841 NN denotes research
T1457 3842-3847 VBZ denotes shows
T1458 3848-3852 IN denotes that
T1459 3853-3868 NN denotes phosphorylation
T1460 3869-3871 IN denotes of
T1461 3872-3877 NNP denotes NF-κB
T1462 3878-3881 NNS denotes p65
T1463 3882-3884 IN denotes at
T1464 3885-3891 NNP denotes Ser276
T1465 3891-3892 , denotes ,
T1466 3893-3899 NNP denotes Ser529
T1467 3900-3902 CC denotes or
T1468 3903-3909 NNP denotes Ser536
T1469 3910-3915 VBZ denotes plays
T1470 3916-3918 DT denotes an
T1471 3919-3928 JJ denotes important
T1472 3929-3933 NN denotes role
T1473 3934-3936 IN denotes in
T1474 3937-3947 VBG denotes regulating
T1475 3948-3963 JJ denotes transcriptional
T1476 3964-3972 NN denotes activity
T1477 3973-3975 IN denotes of
T1478 3976-3981 NNP denotes NF-κB
T1479 3982-3983 NNP denotes [
T1480 3983-3985 CD denotes 17
T1481 3985-3986 NNP denotes ]
T1482 3986-3987 . denotes .
T1483 3988-3990 IN denotes In
T1484 3991-4003 JJ denotes TNFα-treated
T1485 4004-4010 NN denotes murine
T1486 4011-4022 NNS denotes fibroblasts
T1487 4022-4023 , denotes ,
T1488 4024-4030 NNP denotes Ser276
T1489 4031-4033 IN denotes of
T1490 4034-4039 NNP denotes NF-κB
T1491 4040-4043 CD denotes p65
T1492 4044-4046 VBZ denotes is
T1493 4047-4061 VBN denotes phosphorylated
T1494 4062-4064 IN denotes by
T1495 4065-4069 CD denotes MSK1
T1496 4070-4072 TO denotes to
T1497 4073-4080 VB denotes enhance
T1498 4081-4086 JJ denotes NF-κB
T1499 4087-4102 JJ denotes transcriptional
T1500 4103-4111 NN denotes activity
T1501 4112-4113 NNP denotes [
T1502 4113-4115 CD denotes 18
T1503 4115-4116 NNP denotes ]
T1504 4116-4117 . denotes .
T1505 4118-4120 IN denotes In
T1506 4121-4132 NNS denotes macrophages
T1507 4133-4140 VBN denotes treated
T1508 4141-4145 IN denotes with
T1509 4146-4155 NN denotes endotoxin
T1510 4155-4156 , denotes ,
T1511 4157-4162 JJ denotes NF-κB
T1512 4163-4176 NN denotes transcription
T1513 4177-4185 NN denotes activity
T1514 4186-4188 VBZ denotes is
T1515 4189-4199 VBN denotes associated
T1516 4200-4204 IN denotes with
T1517 4205-4220 NN denotes phosphorylation
T1518 4221-4223 IN denotes on
T1519 4224-4230 NNP denotes Ser276
T1520 4231-4234 CC denotes and
T1521 4235-4241 NNP denotes Ser536
T1522 4242-4246 WDT denotes that
T1523 4247-4249 VBZ denotes is
T1524 4250-4259 VBN denotes regulated
T1525 4260-4267 IN denotes through
T1526 4268-4275 NN denotes protein
T1527 4276-4282 NN denotes kinase
T1528 4283-4284 DT denotes A
T1529 4285-4286 -LRB- denotes (
T1530 4286-4289 NNP denotes PKA
T1531 4289-4290 -RRB- denotes )
T1532 4291-4294 CC denotes and
T1533 4295-4299 NNP denotes IKKβ
T1534 4300-4312 RB denotes respectively
T1535 4312-4313 NNP denotes [
T1536 4313-4315 CD denotes 16
T1537 4315-4316 NNP denotes ]
T1538 4316-4317 , denotes ,
T1539 4318-4319 NNP denotes [
T1540 4319-4321 CD denotes 19
T1541 4321-4322 NNP denotes ]
T1542 4322-4323 . denotes .
T1543 4324-4326 IN denotes In
T1544 4327-4332 JJ denotes human
T1545 4333-4334 NNP denotes T
T1546 4335-4340 NNS denotes cells
T1547 4340-4341 , denotes ,
T1548 4342-4347 NNP denotes NF-κB
T1549 4348-4351 CD denotes p65
T1550 4352-4354 VBZ denotes is
T1551 4355-4369 RB denotes constitutively
T1552 4370-4384 VBN denotes phosphorylated
T1553 4385-4387 IN denotes on
T1554 4388-4394 CD denotes Ser536
T1555 4395-4397 TO denotes to
T1556 4398-4408 VB denotes facilitate
T1557 4409-4414 NNP denotes NF-κB
T1558 4415-4418 CD denotes p65
T1559 4419-4426 JJ denotes nuclear
T1560 4427-4440 NN denotes translocation
T1561 4441-4450 VBG denotes following
T1562 4451-4459 JJ denotes cellular
T1563 4460-4471 NN denotes stimulation
T1564 4472-4473 NNP denotes [
T1565 4473-4475 CD denotes 20
T1566 4475-4476 NNP denotes ]
T1567 4476-4477 . denotes .
T1568 4478-4490 VBG denotes Accumulating
T1569 4491-4499 NN denotes evidence
T1570 4500-4507 VBZ denotes reveals
T1571 4508-4512 IN denotes that
T1572 4513-4518 NNP denotes NF-κB
T1573 4519-4522 CD denotes p65
T1574 4523-4538 NN denotes phosphorylation
T1575 4539-4541 IN denotes at
T1576 4542-4548 NNP denotes Ser276
T1577 4549-4551 VBZ denotes is
T1578 4552-4559 JJ denotes crucial
T1579 4560-4563 IN denotes for
T1580 4564-4567 PRP$ denotes its
T1581 4568-4583 JJ denotes transcriptional
T1582 4584-4592 NN denotes activity
T1583 4592-4593 . denotes .
T1584 4594-4598 IN denotes Upon
T1585 4599-4606 JJ denotes nuclear
T1586 4607-4620 NN denotes translocation
T1587 4620-4621 , denotes ,
T1588 4622-4637 NN denotes phosphorylation
T1589 4638-4640 IN denotes of
T1590 4641-4647 NNP denotes Ser276
T1591 4648-4650 IN denotes on
T1592 4651-4656 NNP denotes NF-κB
T1593 4657-4660 CD denotes p65
T1594 4661-4663 IN denotes by
T1595 4664-4667 NNP denotes PKA
T1596 4668-4676 VBZ denotes recruits
T1597 4677-4680 DT denotes the
T1598 4681-4694 NN denotes transcription
T1599 4695-4707 NN denotes co-activator
T1600 4707-4708 , denotes ,
T1601 4709-4713 CD denotes p300
T1602 4714-4716 TO denotes to
T1603 4717-4727 VB denotes potentiate
T1604 4728-4743 JJ denotes NF-κB-regulated
T1605 4744-4748 NN denotes gene
T1606 4749-4762 NN denotes transcription
T1607 4763-4764 NNP denotes [
T1608 4764-4766 CD denotes 21
T1609 4766-4767 NNP denotes ]
T1610 4767-4768 . denotes .
T1611 4769-4776 RB denotes However
T1612 4776-4777 , denotes ,
T1613 4778-4783 JJ denotes other
T1614 4784-4791 NNS denotes studies
T1615 4792-4796 VBP denotes show
T1616 4797-4801 IN denotes that
T1617 4802-4805 NNP denotes PKA
T1618 4806-4814 VBZ denotes inhibits
T1619 4815-4830 JJ denotes NF-κB-regulated
T1620 4831-4835 NN denotes gene
T1621 4836-4846 NN denotes expression
T1622 4847-4849 IN denotes by
T1623 4850-4861 VBG denotes stabilizing
T1624 4862-4866 NNP denotes IκBα
T1625 4867-4868 NNP denotes [
T1626 4868-4870 CD denotes 22
T1627 4870-4871 NNP denotes ]
T1628 4871-4872 , denotes ,
T1629 4873-4874 NNP denotes [
T1630 4874-4876 CD denotes 23
T1631 4876-4877 NNP denotes ]
T1632 4877-4878 . denotes .
T1633 4879-4892 RB denotes Interestingly
T1634 4892-4893 , denotes ,
T1635 4894-4897 DT denotes the
T1636 4898-4914 JJ denotes serine/threonine
T1637 4915-4921 NN denotes kinase
T1638 4922-4927 NN denotes Pim-1
T1639 4928-4936 RB denotes directly
T1640 4937-4951 VBZ denotes phosphorylates
T1641 4952-4957 NNP denotes NF-κB
T1642 4958-4961 CD denotes p65
T1643 4962-4964 IN denotes at
T1644 4965-4971 CD denotes Ser276
T1645 4972-4974 IN denotes by
T1646 4975-4986 VBG denotes stabilizing
T1647 4987-4989 TO denotes to
T1648 4990-4997 VB denotes prevent
T1649 4998-5018 JJ denotes ubiquitin-proteasome
T1650 5019-5030 NN denotes degradation
T1651 5031-5032 NNP denotes [
T1652 5032-5034 CD denotes 24
T1653 5034-5035 NNP denotes ]
T1654 5035-5036 . denotes .
T1655 5037-5044 JJ denotes Several
T1656 5045-5050 JJ denotes other
T1657 5051-5066 NN denotes phosphorylation
T1658 5067-5072 NNS denotes sites
T1659 5073-5076 VBP denotes are
T1660 5077-5081 RB denotes also
T1661 5082-5091 VBN denotes described
T1662 5092-5094 TO denotes to
T1663 5095-5102 VB denotes enhance
T1664 5103-5108 JJ denotes NF-κB
T1665 5109-5113 NN denotes gene
T1666 5114-5129 NN denotes transactivation
T1667 5130-5131 NNP denotes [
T1668 5131-5133 CD denotes 25
T1669 5133-5134 NNP denotes ]
T1670 5134-5135 . denotes .
T1671 5136-5140 RB denotes Here
T1672 5140-5141 , denotes ,
T1673 5142-5144 PRP denotes we
T1674 5145-5156 VBP denotes investigate
T1675 5157-5160 DT denotes the
T1676 5161-5165 NN denotes role
T1677 5166-5168 IN denotes of
T1678 5169-5176 NN denotes protein
T1679 5177-5183 NN denotes kinase
T1680 5184-5185 NNP denotes C
T1681 5186-5187 -LRB- denotes (
T1682 5187-5190 NNP denotes PKC
T1683 5190-5191 -RRB- denotes )
T1684 5192-5194 IN denotes in
T1685 5195-5211 NNP denotes M-CSF-stimulated
T1686 5212-5217 NNP denotes NF-κB
T1687 5218-5228 NN denotes activation
T1688 5228-5229 . denotes .
T1689 5230-5233 NNP denotes PKC
T1690 5234-5242 NNS denotes proteins
T1691 5243-5246 VBP denotes are
T1692 5247-5262 JJ denotes multifunctional
T1693 5263-5270 NNS denotes kinases
T1694 5271-5275 WDT denotes that
T1695 5276-5282 VBP denotes differ
T1696 5283-5285 IN denotes in
T1697 5286-5295 NN denotes structure
T1698 5295-5296 , denotes ,
T1699 5297-5305 NN denotes function
T1700 5306-5309 CC denotes and
T1701 5310-5319 NN denotes co-factor
T1702 5320-5331 NN denotes requirement
T1703 5332-5333 NNP denotes [
T1704 5333-5335 CD denotes 26
T1705 5335-5336 NNP denotes ]
T1706 5336-5337 . denotes .
T1707 5338-5342 NNS denotes PKCs
T1708 5343-5346 VBP denotes are
T1709 5347-5355 VBN denotes involved
T1710 5356-5358 IN denotes in
T1711 5359-5366 JJ denotes diverse
T1712 5367-5371 NN denotes cell
T1713 5372-5381 NNS denotes responses
T1714 5381-5382 , denotes ,
T1715 5383-5392 VBG denotes including
T1716 5393-5397 NN denotes cell
T1717 5398-5404 NN denotes growth
T1718 5404-5405 , denotes ,
T1719 5406-5414 NN denotes survival
T1720 5414-5415 , denotes ,
T1721 5416-5431 NN denotes differentiation
T1722 5432-5435 CC denotes and
T1723 5436-5447 NN denotes development
T1724 5448-5449 NNP denotes [
T1725 5449-5451 CD denotes 27
T1726 5451-5452 NNP denotes ]
T1727 5452-5453 . denotes .
T1728 5454-5457 DT denotes The
T1729 5458-5460 CD denotes 12
T1730 5461-5468 RB denotes closely
T1731 5469-5476 JJ denotes related
T1732 5477-5484 NNS denotes enzymes
T1733 5485-5487 IN denotes of
T1734 5488-5491 DT denotes the
T1735 5492-5495 NNP denotes PKC
T1736 5496-5502 NN denotes family
T1737 5503-5506 VBP denotes are
T1738 5507-5514 VBN denotes divided
T1739 5515-5519 IN denotes into
T1740 5520-5525 CD denotes three
T1741 5526-5533 NNS denotes classes
T1742 5533-5534 : denotes :
T1743 5535-5547 JJ denotes conventional
T1744 5548-5549 -LRB- denotes (
T1745 5549-5554 NNS denotes cPKCs
T1746 5554-5555 : denotes :
T1747 5556-5557 VB denotes α
T1748 5558-5560 NNP denotes βI
T1749 5561-5564 NNP denotes βII
T1750 5565-5568 CC denotes and
T1751 5569-5570 NNP denotes γ
T1752 5571-5578 VBP denotes require
T1753 5579-5582 JJ denotes Ca2
T1754 5582-5583 NN denotes +
T1755 5584-5587 CC denotes and
T1756 5588-5602 NN denotes diacylglycerol
T1757 5603-5604 -LRB- denotes (
T1758 5604-5607 NNP denotes DAG
T1759 5607-5608 -RRB- denotes )
T1760 5608-5609 : denotes ;
T1761 5610-5615 NN denotes novel
T1762 5616-5617 -LRB- denotes (
T1763 5617-5622 NNS denotes nPKCs
T1764 5622-5623 : denotes :
T1765 5624-5625 VB denotes δ
T1766 5625-5626 , denotes ,
T1767 5627-5628 VB denotes ε
T1768 5628-5629 , denotes ,
T1769 5630-5631 VB denotes η
T1770 5631-5632 , denotes ,
T1771 5633-5634 VB denotes θ
T1772 5635-5638 CC denotes and
T1773 5639-5640 VB denotes μ
T1774 5640-5641 -RRB- denotes )
T1775 5642-5649 VBP denotes require
T1776 5650-5653 NNP denotes DAG
T1777 5653-5654 : denotes ;
T1778 5655-5658 CC denotes and
T1779 5659-5667 JJ denotes atypical
T1780 5668-5669 -LRB- denotes (
T1781 5669-5674 NNS denotes aPKCs
T1782 5674-5675 : denotes :
T1783 5676-5677 VB denotes ξ
T1784 5677-5678 , denotes ,
T1785 5679-5680 VB denotes ι
T1786 5681-5684 CC denotes and
T1787 5685-5686 VB denotes λ
T1788 5686-5687 -RRB- denotes )
T1789 5688-5695 VBP denotes require
T1790 5696-5703 CC denotes neither
T1791 5704-5707 VBP denotes Ca2
T1792 5707-5708 CD denotes +
T1793 5709-5712 CC denotes nor
T1794 5713-5716 NNP denotes DAG
T1795 5716-5717 . denotes .
T1796 5718-5727 NNS denotes Monocytes
T1797 5728-5731 CC denotes and
T1798 5732-5743 NNS denotes macrophages
T1799 5744-5757 RB denotes predominantly
T1800 5758-5765 VBP denotes express
T1801 5766-5778 JJ denotes conventional
T1802 5779-5782 NNP denotes PKC
T1803 5783-5791 NNS denotes isoforms
T1804 5792-5793 -LRB- denotes (
T1805 5793-5797 NNP denotes PKCα
T1806 5798-5803 NNP denotes PKCβI
T1807 5804-5807 CC denotes and
T1808 5808-5814 NNP denotes PKCβII
T1809 5814-5815 -RRB- denotes )
T1810 5816-5819 CC denotes and
T1811 5820-5825 JJ denotes novel
T1812 5826-5830 NNS denotes PKCs
T1813 5831-5832 -LRB- denotes (
T1814 5832-5836 NNP denotes PKCδ
T1815 5837-5840 CC denotes and
T1816 5841-5845 NNP denotes PKCε
T1817 5845-5846 -RRB- denotes )
T1818 5846-5847 . denotes .
T1819 5848-5860 JJ denotes Conventional
T1820 5861-5865 NNS denotes PKCs
T1821 5866-5874 VB denotes regulate
T1822 5875-5890 NN denotes differentiation
T1823 5891-5893 IN denotes of
T1824 5894-5897 DT denotes the
T1825 5898-5903 JJ denotes human
T1826 5904-5917 JJ denotes promyelocytic
T1827 5918-5926 NN denotes leukemia
T1828 5927-5931 NN denotes cell
T1829 5932-5936 NN denotes line
T1830 5937-5941 CD denotes HL60
T1831 5942-5944 TO denotes to
T1832 5945-5956 NNS denotes macrophages
T1833 5957-5958 NNP denotes [
T1834 5958-5960 CD denotes 28
T1835 5960-5961 NNP denotes ]
T1836 5961-5962 . denotes .
T1837 5963-5967 NNP denotes PKCα
T1838 5968-5975 VBZ denotes induces
T1839 5976-5981 NNP denotes IL-1α
T1840 5981-5982 , denotes ,
T1841 5983-5987 NNP denotes iNOS
T1842 5988-5991 CC denotes and
T1843 5992-5996 NNP denotes TNFα
T1844 5997-6001 NNP denotes mRNA
T1845 6002-6012 NN denotes production
T1846 6013-6018 IN denotes after
T1847 6019-6037 NN denotes lipopolysaccharide
T1848 6038-6039 -LRB- denotes (
T1849 6039-6042 NNP denotes LPS
T1850 6042-6043 -RRB- denotes )
T1851 6044-6052 NN denotes exposure
T1852 6053-6054 NNP denotes [
T1853 6054-6056 CD denotes 29
T1854 6056-6057 NNP denotes ]
T1855 6057-6058 . denotes .
T1856 6059-6061 IN denotes In
T1857 6062-6070 NN denotes addition
T1858 6070-6071 , denotes ,
T1859 6072-6084 VBG denotes accumulating
T1860 6085-6093 NN denotes evidence
T1861 6094-6102 VBZ denotes suggests
T1862 6103-6107 IN denotes that
T1863 6108-6120 JJ denotes conventional
T1864 6121-6125 NNS denotes PKCs
T1865 6126-6130 IN denotes like
T1866 6131-6135 NNP denotes PKCα
T1867 6136-6140 VBP denotes have
T1868 6141-6155 JJ denotes anti-apoptotic
T1869 6156-6165 NNS denotes functions
T1870 6165-6166 . denotes .
T1871 6167-6170 IN denotes For
T1872 6171-6178 NN denotes example
T1873 6178-6179 , denotes ,
T1874 6180-6184 NNP denotes PKCα
T1875 6185-6187 VBZ denotes is
T1876 6188-6201 VBN denotes overexpressed
T1877 6202-6204 IN denotes in
T1878 6205-6206 DT denotes a
T1879 6207-6214 NN denotes variety
T1880 6215-6217 IN denotes of
T1881 6218-6223 NN denotes tumor
T1882 6224-6229 NNS denotes cells
T1883 6230-6239 VBG denotes including
T1884 6240-6247 NNS denotes gliomas
T1885 6247-6248 , denotes ,
T1886 6249-6254 NN denotes liver
T1887 6254-6255 , denotes ,
T1888 6256-6259 CC denotes and
T1889 6260-6264 NN denotes lung
T1890 6265-6266 NNP denotes [
T1891 6266-6268 CD denotes 30
T1892 6268-6269 NNP denotes ]
T1893 6269-6270 , denotes ,
T1894 6271-6272 NNP denotes [
T1895 6272-6274 CD denotes 31
T1896 6274-6275 NNP denotes ]
T1897 6275-6276 . denotes .
T1898 6277-6279 IN denotes In
T1899 6280-6290 JJ denotes epithelial
T1900 6291-6296 NNS denotes cells
T1901 6296-6297 , denotes ,
T1902 6298-6308 NN denotes inhibition
T1903 6309-6311 IN denotes of
T1904 6312-6316 NNP denotes PKCα
T1905 6317-6324 VBZ denotes induces
T1906 6325-6339 JJ denotes PKCδ-dependent
T1907 6340-6349 NN denotes apoptosis
T1908 6350-6351 NNP denotes [
T1909 6351-6353 CD denotes 31
T1910 6353-6354 NNP denotes ]
T1911 6354-6355 . denotes .
T1912 6356-6369 RB denotes Interestingly
T1913 6369-6370 , denotes ,
T1914 6371-6373 IN denotes in
T1915 6374-6379 JJ denotes human
T1916 6380-6389 NNS denotes monocytes
T1917 6390-6393 CC denotes and
T1918 6394-6406 JJ denotes premonocytic
T1919 6407-6412 NN denotes THP-1
T1920 6413-6421 NN denotes leukemia
T1921 6422-6427 NNS denotes cells
T1922 6427-6428 , denotes ,
T1923 6429-6434 NN denotes novel
T1924 6435-6439 NNS denotes PKCs
T1925 6440-6444 IN denotes like
T1926 6445-6449 NNP denotes PKCδ
T1927 6450-6454 VBP denotes have
T1928 6455-6458 DT denotes the
T1929 6459-6467 JJ denotes opposite
T1930 6468-6474 NN denotes effect
T1931 6475-6477 IN denotes on
T1932 6478-6482 NN denotes cell
T1933 6483-6491 NN denotes survival
T1934 6491-6492 , denotes ,
T1935 6493-6497 NNP denotes Voss
T1936 6498-6500 FW denotes et
T1937 6501-6503 FW denotes al
T1938 6504-6510 VBD denotes showed
T1939 6511-6515 IN denotes that
T1940 6516-6520 NNP denotes PKCδ
T1941 6521-6529 RB denotes directly
T1942 6530-6544 VBZ denotes phosphorylates
T1943 6545-6554 JJ denotes caspase-3
T1944 6555-6558 CC denotes and
T1945 6559-6567 VBZ denotes promotes
T1946 6568-6585 JJ denotes etoposide-induced
T1947 6586-6595 NN denotes apoptosis
T1948 6596-6597 NNP denotes [
T1949 6597-6599 CD denotes 32
T1950 6599-6600 NNP denotes ]
T1951 6600-6601 . denotes .
T1952 6602-6610 RB denotes Moreover
T1953 6610-6611 , denotes ,
T1954 6612-6620 NN denotes knockout
T1955 6621-6626 NN denotes mouse
T1956 6627-6634 NNS denotes studies
T1957 6635-6642 VBP denotes suggest
T1958 6643-6647 IN denotes that
T1959 6648-6655 DT denotes another
T1960 6656-6661 NN denotes novel
T1961 6662-6665 NNP denotes PKC
T1962 6665-6666 , denotes ,
T1963 6667-6671 NNP denotes PKCε
T1964 6672-6674 VBZ denotes is
T1965 6675-6685 RB denotes critically
T1966 6686-6694 VBN denotes involved
T1967 6695-6697 IN denotes in
T1968 6698-6703 JJ denotes early
T1969 6704-6716 JJ denotes LPS-mediated
T1970 6717-6726 VBG denotes signaling
T1971 6727-6729 IN denotes in
T1972 6730-6739 VBN denotes activated
T1973 6740-6751 NNS denotes macrophages
T1974 6752-6753 NNP denotes [
T1975 6753-6755 CD denotes 33
T1976 6755-6756 NNP denotes ]
T1977 6756-6757 . denotes .
T1978 6758-6768 RB denotes Previously
T1979 6768-6769 , denotes ,
T1980 6770-6772 PRP denotes we
T1981 6773-6781 VBD denotes reported
T1982 6782-6786 IN denotes that
T1983 6787-6792 NNP denotes M-CSF
T1984 6793-6801 VBZ denotes promotes
T1985 6802-6810 NN denotes monocyte
T1986 6811-6819 NN denotes survival
T1987 6820-6827 IN denotes through
T1988 6828-6831 DT denotes the
T1989 6832-6842 NN denotes activation
T1990 6843-6845 IN denotes of
T1991 6846-6849 DT denotes the
T1992 6850-6859 NN denotes PI3-K/AKT
T1993 6860-6867 NN denotes pathway
T1994 6868-6869 NNP denotes [
T1995 6869-6870 CD denotes 3
T1996 6870-6871 NNP denotes ]
T1997 6871-6872 . denotes .
T1998 6873-6875 IN denotes In
T1999 6876-6884 NN denotes addition
T2000 6885-6887 TO denotes to
T2001 6888-6891 NNP denotes AKT
T2002 6892-6902 NN denotes activation
T2003 6902-6903 , denotes ,
T2004 6904-6909 NNP denotes M-CSF
T2005 6910-6920 VBZ denotes stimulates
T2006 6921-6924 NNP denotes PKC
T2007 6925-6927 IN denotes in
T2008 6928-6933 JJ denotes human
T2009 6934-6943 NNS denotes monocytes
T2010 6944-6947 CC denotes and
T2011 6948-6957 NNS denotes increases
T2012 6958-6963 NNP denotes NF-κB
T2013 6964-6967 NNP denotes DNA
T2014 6968-6975 JJ denotes binding
T2015 6976-6977 NNP denotes [
T2016 6977-6979 CD denotes 34
T2017 6979-6980 NNP denotes ]
T2018 6980-6981 . denotes .
T2019 6982-6989 RB denotes However
T2020 6989-6990 , denotes ,
T2021 6991-6998 IN denotes whether
T2022 6999-7002 NNP denotes PKC
T2023 7003-7009 CC denotes and/or
T2024 7010-7015 NNP denotes NF-κB
T2025 7016-7026 NN denotes activation
T2026 7027-7029 VBZ denotes is
T2027 7030-7038 JJ denotes critical
T2028 7039-7041 IN denotes in
T2029 7042-7058 JJ denotes M-CSF-stimulated
T2030 7059-7070 NN denotes mononuclear
T2031 7071-7080 NN denotes phagocyte
T2032 7081-7089 NN denotes survival
T2033 7090-7096 CC denotes and/or
T2034 7097-7112 NN denotes differentiation
T2035 7113-7115 VBZ denotes is
T2036 7116-7123 JJ denotes unclear
T2037 7123-7124 . denotes .
T2038 7125-7127 IN denotes In
T2039 7128-7133 JJ denotes other
T2040 7134-7139 NNS denotes cells
T2041 7139-7140 , denotes ,
T2042 7141-7144 NNP denotes PKC
T2043 7145-7150 VBZ denotes plays
T2044 7151-7153 DT denotes an
T2045 7154-7163 JJ denotes important
T2046 7164-7168 NN denotes role
T2047 7169-7171 IN denotes in
T2048 7172-7177 JJ denotes NF-κB
T2049 7178-7188 NN denotes activation
T2050 7189-7192 CC denotes and
T2051 7193-7197 NN denotes cell
T2052 7198-7206 NN denotes survival
T2053 7207-7208 NNP denotes [
T2054 7208-7210 CD denotes 35
T2055 7210-7211 NNP denotes ]
T2056 7211-7212 , denotes ,
T2057 7213-7220 RB denotes however
T2058 7221-7224 DT denotes the
T2059 7225-7233 JJ denotes specific
T2060 7234-7244 NNS denotes mechanisms
T2061 7245-7247 IN denotes of
T2062 7248-7252 DT denotes this
T2063 7253-7263 NN denotes activation
T2064 7264-7267 CC denotes and
T2065 7268-7271 DT denotes the
T2066 7272-7282 JJ denotes biological
T2067 7283-7290 NNS denotes effects
T2068 7291-7293 IN denotes on
T2069 7294-7302 JJ denotes cellular
T2070 7303-7312 NN denotes phenotype
T2071 7313-7316 VBP denotes are
T2072 7317-7320 RB denotes not
T2073 7321-7326 VBN denotes known
T2074 7326-7327 . denotes .
T2075 7328-7337 RB denotes Therefore
T2076 7337-7338 , denotes ,
T2077 7339-7341 PRP denotes we
T2078 7342-7349 VBD denotes focused
T2079 7350-7352 IN denotes at
T2080 7353-7366 VBG denotes understanding
T2081 7367-7370 DT denotes the
T2082 7371-7375 NN denotes role
T2083 7376-7378 IN denotes of
T2084 7379-7382 NNP denotes PKC
T2085 7383-7385 IN denotes in
T2086 7386-7389 DT denotes the
T2087 7390-7400 NN denotes regulation
T2088 7401-7403 IN denotes of
T2089 7404-7409 JJ denotes NF-κB
T2090 7410-7420 NN denotes activation
T2091 7421-7424 CC denotes and
T2092 7425-7438 JJ denotes M-CSF-induced
T2093 7439-7447 NN denotes monocyte
T2094 7448-7456 NN denotes survival
T2095 7456-7457 . denotes .
T2096 7458-7462 RB denotes Here
T2097 7463-7465 PRP denotes we
T2098 7466-7478 VBZ denotes demonstrates
T2099 7479-7483 IN denotes that
T2100 7484-7489 NNP denotes M-CSF
T2101 7490-7501 VBD denotes upregulated
T2102 7502-7505 DT denotes the
T2103 7506-7511 JJ denotes NF-κB
T2104 7512-7525 NN denotes transcription
T2105 7526-7529 CC denotes and
T2106 7530-7534 NN denotes cell
T2107 7535-7543 NN denotes survival
T2108 7544-7546 IN denotes in
T2109 7547-7552 JJ denotes human
T2110 7553-7556 CC denotes and
T2111 7557-7562 NN denotes mouse
T2112 7563-7574 NNS denotes macrophages
T2113 7574-7575 . denotes .
T2114 7576-7580 DT denotes This
T2115 7581-7589 NN denotes activity
T2116 7590-7593 VBD denotes was
T2117 7594-7601 VBN denotes reduced
T2118 7602-7604 IN denotes by
T2119 7605-7608 DT denotes the
T2120 7609-7621 JJ denotes conventional
T2121 7621-7622 , denotes ,
T2122 7623-7626 CC denotes but
T2123 7627-7630 RB denotes not
T2124 7631-7636 JJ denotes novel
T2125 7636-7637 , denotes ,
T2126 7638-7641 NNP denotes PKC
T2127 7642-7652 NNS denotes inhibitors
T2128 7652-7653 , denotes ,
T2129 7654-7662 JJ denotes dominant
T2130 7663-7671 JJ denotes negative
T2131 7672-7676 NNP denotes PKCα
T2132 7677-7687 NNS denotes constructs
T2133 7688-7690 CC denotes or
T2134 7691-7695 NNP denotes PKCα
T2135 7696-7701 NNP denotes siRNA
T2136 7701-7702 . denotes .
T2137 7703-7715 JJ denotes Conventional
T2138 7716-7719 NNP denotes PKC
T2139 7720-7729 VBN denotes regulated
T2140 7730-7745 JJ denotes NF-κB-regulated
T2141 7746-7750 NN denotes gene
T2142 7751-7761 NN denotes expression
T2143 7762-7765 CC denotes and
T2144 7766-7781 NN denotes phosphorylation
T2145 7782-7784 IN denotes of
T2146 7785-7791 NNP denotes Ser276
T2147 7792-7794 IN denotes of
T2148 7795-7800 NNP denotes NF-κB
T2149 7801-7804 CD denotes p65
T2150 7805-7813 VBD denotes occurred
T2151 7814-7816 IN denotes in
T2152 7817-7819 DT denotes an
T2153 7820-7835 JJ denotes M-CSF-dependent
T2154 7836-7843 NN denotes fashion
T2155 7844-7855 VBG denotes correlating
T2156 7856-7860 IN denotes with
T2157 7861-7864 PRP$ denotes its
T2158 7865-7872 JJ denotes maximal
T2159 7873-7888 JJ denotes transcriptional
T2160 7889-7897 NN denotes activity
T2161 7897-7898 . denotes .
T2162 7899-7910 RB denotes Furthermore
T2163 7910-7911 , denotes ,
T2164 7912-7926 JJ denotes PKCα-regulated
T2165 7927-7942 NN denotes phosphorylation
T2166 7943-7945 IN denotes of
T2167 7946-7952 NNP denotes Ser276
T2168 7953-7955 IN denotes of
T2169 7956-7961 NNP denotes NF-κB
T2170 7962-7965 CD denotes p65
T2171 7966-7971 VBZ denotes plays
T2172 7972-7974 DT denotes an
T2173 7975-7984 JJ denotes important
T2174 7985-7989 NN denotes role
T2175 7990-7992 IN denotes in
T2176 7993-8003 VBG denotes regulating
T2177 8004-8007 PRP$ denotes its
T2178 8008-8016 NN denotes activity
T2179 8017-8019 IN denotes in
T2180 8020-8031 NN denotes mononuclear
T2181 8032-8042 NNS denotes phagocytes
T2182 8043-8046 CC denotes and
T2183 8047-8053 JJ denotes murine
T2184 8054-8063 JJ denotes embryonic
T2185 8064-8075 NNS denotes fibroblasts
T2186 8075-8076 . denotes .
T4674 8087-8092 NNP denotes M-CSF
T4675 8093-8100 VBZ denotes Induces
T4676 8101-8106 NNP denotes NF-κB
T4677 8107-8122 NNP denotes Transcriptional
T4678 8123-8131 NNP denotes Activity
T4679 8132-8134 IN denotes in
T4680 8135-8140 NNP denotes Human
T4681 8141-8149 NNP denotes Monocyte
T4682 8150-8157 NNP denotes Derived
T4683 8158-8169 NNP denotes Macrophages
T4684 8170-8171 -LRB- denotes (
T4685 8171-8175 NNP denotes MDMs
T4686 8175-8176 -RRB- denotes )
T4687 8177-8180 CC denotes and
T4688 8181-8186 NNP denotes Mouse
T4689 8187-8197 NNP denotes Macrophage
T4690 8198-8202 NNP denotes Cell
T4691 8203-8207 NNP denotes Line
T4692 8207-8208 , denotes ,
T4693 8209-8212 NNP denotes RAW
T4694 8213-8218 CD denotes 264.7
T4695 8219-8221 TO denotes To
T4696 8222-8231 VB denotes determine
T4697 8232-8234 IN denotes if
T4698 8235-8240 NNP denotes M-CSF
T4699 8241-8248 VBD denotes induced
T4700 8249-8254 NNP denotes NF-κB
T4701 8255-8258 NNP denotes DNA
T4702 8259-8266 JJ denotes binding
T4703 8267-8269 IN denotes in
T4704 8270-8275 JJ denotes human
T4705 8276-8287 NNS denotes macrophages
T4706 8287-8288 , denotes ,
T4707 8289-8291 PRP denotes we
T4708 8292-8301 VBD denotes performed
T4709 8302-8306 NNP denotes EMSA
T4710 8307-8315 NN denotes analysis
T4711 8316-8318 IN denotes on
T4712 8319-8326 JJ denotes nuclear
T4713 8327-8334 NNS denotes lysates
T4714 8335-8339 IN denotes from
T4715 8340-8353 JJ denotes M-CSF-treated
T4716 8354-8358 NNS denotes MDMs
T4717 8358-8359 . denotes .
T4718 8360-8367 JJ denotes Similar
T4719 8368-8370 TO denotes to
T4720 8371-8379 JJ denotes previous
T4721 8380-8387 NNS denotes reports
T4722 8388-8389 NNP denotes [
T4723 8389-8391 CD denotes 11
T4724 8391-8392 NNP denotes ]
T4725 8392-8393 , denotes ,
T4726 8394-8401 JJ denotes nuclear
T4727 8402-8407 NN denotes NF-κB
T4728 8408-8422 RB denotes constitutively
T4729 8423-8428 VBN denotes bound
T4730 8429-8432 NN denotes DNA
T4731 8433-8435 IN denotes in
T4732 8436-8450 JJ denotes non-stimulated
T4733 8451-8460 NNS denotes monocytes
T4734 8461-8462 -LRB- denotes (
T4735 8462-8468 NN denotes Figure
T4736 8469-8471 CD denotes 1A
T4737 8471-8472 -RRB- denotes )
T4738 8472-8473 . denotes .
T4739 8474-8487 RB denotes Interestingly
T4740 8487-8488 , denotes ,
T4741 8489-8495 VBG denotes adding
T4742 8496-8501 NNP denotes M-CSF
T4743 8502-8505 VBD denotes did
T4744 8506-8509 RB denotes not
T4745 8510-8515 VB denotes alter
T4746 8516-8521 NNP denotes NF-κB
T4747 8522-8525 NNP denotes DNA
T4748 8526-8533 JJ denotes binding
T4749 8534-8536 IN denotes by
T4750 8537-8541 NNP denotes EMSA
T4751 8541-8542 . denotes .
T4752 8543-8545 IN denotes In
T4753 8546-8554 NN denotes contrast
T4754 8554-8555 , denotes ,
T4755 8556-8561 IN denotes after
T4756 8562-8573 RB denotes transiently
T4757 8574-8586 VBG denotes transfecting
T4758 8587-8592 JJ denotes human
T4759 8593-8597 NNS denotes MDMs
T4760 8598-8602 IN denotes with
T4761 8603-8614 JJ denotes pNF-κB-SEAP
T4762 8615-8625 NNS denotes constructs
T4763 8626-8636 VBG denotes containing
T4764 8637-8641 CD denotes four
T4765 8642-8647 JJ denotes NF-κB
T4766 8648-8657 NN denotes consensus
T4767 8658-8665 JJ denotes binding
T4768 8666-8675 NNS denotes sequences
T4769 8675-8676 , denotes ,
T4770 8677-8682 NNP denotes M-CSF
T4771 8683-8692 NN denotes treatment
T4772 8693-8695 IN denotes of
T4773 8696-8699 DT denotes the
T4774 8700-8711 VBN denotes transfected
T4775 8712-8717 NNS denotes cells
T4776 8718-8726 VBD denotes resulted
T4777 8727-8729 IN denotes in
T4778 8730-8731 DT denotes a
T4779 8732-8740 JJ denotes 2.3-fold
T4780 8741-8749 NN denotes increase
T4781 8750-8752 IN denotes in
T4782 8753-8757 NNP denotes SEAP
T4783 8758-8765 NN denotes release
T4784 8766-8768 IN denotes in
T4785 8769-8772 DT denotes the
T4786 8773-8780 NN denotes culture
T4787 8781-8786 NNS denotes media
T4788 8787-8795 VBN denotes compared
T4789 8796-8798 TO denotes to
T4790 8799-8802 NNP denotes PBS
T4791 8803-8804 -LRB- denotes (
T4792 8804-8811 NN denotes vehicle
T4793 8811-8812 -RRB- denotes )
T4794 8812-8813 : denotes -
T4795 8813-8820 VBN denotes treated
T4796 8821-8832 VBN denotes transfected
T4797 8833-8837 NNS denotes MDMs
T4798 8838-8839 -LRB- denotes (
T4799 8839-8845 NN denotes Figure
T4800 8846-8848 NN denotes 1B
T4801 8848-8849 -RRB- denotes )
T4802 8849-8850 . denotes .
T4803 8851-8853 IN denotes As
T4804 8854-8855 DT denotes a
T4805 8856-8863 NN denotes control
T4806 8863-8864 , denotes ,
T4807 8865-8868 DT denotes the
T4808 8869-8878 NNP denotes pTAL-SEAP
T4809 8879-8888 VB denotes construct
T4810 8889-8896 VBG denotes lacking
T4811 8897-8902 JJ denotes NF-κB
T4812 8903-8910 JJ denotes binding
T4813 8911-8916 NNS denotes sites
T4814 8917-8920 VBD denotes was
T4815 8921-8925 VBN denotes used
T4816 8925-8926 . denotes .
T4817 8927-8932 NNS denotes Cells
T4818 8933-8944 VBN denotes transfected
T4819 8945-8949 IN denotes with
T4820 8950-8953 DT denotes the
T4821 8954-8963 NNP denotes pTAL-SEAP
T4822 8964-8973 VBP denotes construct
T4823 8974-8977 VBD denotes did
T4824 8978-8981 RB denotes not
T4825 8982-8989 VB denotes produce
T4826 8990-8994 NNP denotes SEAP
T4827 8995-8997 IN denotes in
T4828 8998-9001 DT denotes the
T4829 9002-9009 NN denotes absence
T4830 9010-9012 CC denotes or
T4831 9013-9021 NN denotes presence
T4832 9022-9024 IN denotes of
T4833 9025-9030 NNP denotes M-CSF
T4834 9031-9032 -LRB- denotes (
T4835 9032-9038 NNP denotes Figure
T4836 9039-9041 NNP denotes 1B
T4837 9041-9042 -RRB- denotes )
T4838 9042-9043 . denotes .
T22818 9088-9093 NNP denotes M-CSF
T22819 9094-9101 VBZ denotes induces
T22820 9102-9107 JJ denotes NF-κB
T22821 9108-9123 JJ denotes transcriptional
T22822 9124-9132 NN denotes activity
T22823 9133-9135 IN denotes in
T22824 9136-9147 NNS denotes macrophages
T22825 9147-9148 . denotes .
T22826 9149-9150 -LRB- denotes (
T22827 9150-9151 NNP denotes A
T22828 9151-9152 -RRB- denotes )
T22829 9153-9159 NNP denotes Nuclei
T22830 9160-9164 VBD denotes were
T22831 9165-9174 VBN denotes extracted
T22832 9175-9179 IN denotes from
T22833 9180-9185 JJ denotes human
T22834 9186-9190 NNS denotes MDMs
T22835 9191-9198 VBN denotes treated
T22836 9199-9206 IN denotes without
T22837 9207-9209 CC denotes or
T22838 9210-9214 IN denotes with
T22839 9215-9220 NNP denotes M-CSF
T22840 9221-9224 IN denotes for
T22841 9225-9227 CD denotes 15
T22842 9228-9230 CC denotes or
T22843 9231-9233 CD denotes 30
T22844 9234-9241 NNS denotes minutes
T22845 9241-9242 . denotes .
T22846 9243-9248 NNP denotes NF-κB
T22847 9249-9252 NNP denotes DNA
T22848 9253-9260 JJ denotes binding
T22849 9261-9269 NN denotes activity
T22850 9270-9273 VBD denotes was
T22851 9274-9282 VBN denotes analyzed
T22852 9283-9285 IN denotes by
T22853 9286-9290 NNP denotes EMSA
T22854 9291-9296 NNP denotes Shown
T22855 9297-9299 VBZ denotes is
T22856 9300-9301 DT denotes a
T22857 9302-9316 JJ denotes representative
T22858 9317-9321 NN denotes blot
T22859 9322-9326 IN denotes from
T22860 9327-9332 CD denotes three
T22861 9333-9344 JJ denotes independent
T22862 9345-9356 NNS denotes experiments
T22863 9356-9357 . denotes .
T22864 9358-9360 NNP denotes NS
T22865 9360-9361 : denotes :
T22866 9362-9376 JJ denotes non-stimulated
T22867 9376-9377 . denotes .
T22868 9378-9379 -LRB- denotes (
T22869 9379-9380 NNP denotes B
T22870 9380-9381 -RRB- denotes )
T22871 9382-9387 NNP denotes Human
T22872 9388-9392 NNP denotes MDMs
T22873 9393-9404 RB denotes transiently
T22874 9405-9416 VBD denotes transfected
T22875 9417-9421 IN denotes with
T22876 9422-9431 JJ denotes pTAL-SEAP
T22877 9432-9434 CC denotes or
T22878 9435-9446 JJ denotes pNF-κB-SEAP
T22879 9447-9457 NNS denotes constructs
T22880 9458-9462 VBD denotes were
T22881 9463-9470 VBN denotes treated
T22882 9471-9475 IN denotes with
T22883 9476-9481 NNP denotes M-CSF
T22884 9482-9484 IN denotes in
T22885 9485-9491 NNP denotes X-vivo
T22886 9492-9498 NN denotes medium
T22887 9499-9502 CC denotes and
T22888 9503-9512 VBN denotes incubated
T22889 9513-9516 IN denotes for
T22890 9517-9518 CD denotes 6
T22891 9519-9524 NNS denotes hours
T22892 9525-9531 IN denotes before
T22893 9532-9535 DT denotes the
T22894 9536-9546 NN denotes collection
T22895 9547-9549 IN denotes of
T22896 9550-9556 NN denotes medium
T22897 9556-9557 . denotes .
T22898 9558-9563 JJ denotes NF-κB
T22899 9564-9572 NN denotes activity
T22900 9573-9576 VBD denotes was
T22901 9577-9585 VBN denotes analyzed
T22902 9586-9588 IN denotes by
T22903 9589-9598 VBG denotes measuring
T22904 9599-9602 DT denotes the
T22905 9603-9609 NN denotes amount
T22906 9610-9612 IN denotes of
T22907 9613-9617 NNP denotes SEAP
T22908 9618-9626 VBD denotes secreted
T22909 9627-9631 IN denotes into
T22910 9632-9635 DT denotes the
T22911 9636-9642 NN denotes medium
T22912 9643-9646 CC denotes and
T22913 9647-9651 NNS denotes data
T22914 9652-9655 VBP denotes are
T22915 9656-9665 VBN denotes expressed
T22916 9666-9668 IN denotes as
T22917 9669-9673 VBP denotes fold
T22918 9674-9682 NN denotes increase
T22919 9683-9685 IN denotes of
T22920 9686-9690 NNP denotes SEAP
T22921 9691-9699 NN denotes activity
T22922 9700-9704 IN denotes over
T22923 9705-9709 DT denotes that
T22924 9710-9712 IN denotes in
T22925 9713-9722 JJ denotes pTAL-SEAP
T22926 9723-9734 VBN denotes transfected
T22927 9735-9742 VBG denotes resting
T22928 9743-9748 NNS denotes cells
T22929 9748-9749 . denotes .
T22930 9750-9751 -LRB- denotes (
T22931 9751-9752 NNP denotes C
T22932 9752-9753 -RRB- denotes )
T22933 9754-9757 NNP denotes RAW
T22934 9758-9763 CD denotes 264.7
T22935 9764-9769 NNS denotes cells
T22936 9770-9774 VBD denotes were
T22937 9775-9786 RB denotes transiently
T22938 9787-9798 VBN denotes transfected
T22939 9799-9803 IN denotes with
T22940 9804-9813 NNP denotes pTAL-SEAP
T22941 9814-9816 CC denotes or
T22942 9817-9828 NNP denotes pNF-κB-SEAP
T22943 9829-9838 VBP denotes construct
T22944 9838-9839 . denotes .
T22945 9840-9845 NNS denotes Cells
T22946 9846-9850 VBD denotes were
T22947 9851-9856 JJ denotes serum
T22948 9857-9864 VBN denotes starved
T22949 9865-9868 IN denotes for
T22950 9869-9870 CD denotes 4
T22951 9871-9876 NNS denotes hours
T22952 9877-9882 RB denotes prior
T22953 9883-9885 TO denotes to
T22954 9886-9887 CD denotes 2
T22955 9888-9893 NNS denotes hours
T22956 9894-9905 NN denotes stimulation
T22957 9906-9910 IN denotes with
T22958 9911-9916 NN denotes mouse
T22959 9917-9928 JJ denotes recombinant
T22960 9929-9934 NNP denotes M-CSF
T22961 9935-9936 -LRB- denotes (
T22962 9936-9939 CD denotes 100
T22963 9940-9945 NN denotes ng/ml
T22964 9945-9946 -RRB- denotes )
T22965 9946-9947 . denotes .
T22966 9948-9955 NNP denotes Culture
T22967 9956-9961 NNS denotes media
T22968 9962-9965 VBD denotes was
T22969 9966-9975 VBN denotes collected
T22970 9976-9978 TO denotes to
T22971 9979-9986 VB denotes measure
T22972 9987-9991 NNP denotes SEAP
T22973 9992-10002 NN denotes production
T22974 10002-10003 . denotes .
T22975 10004-10008 NNS denotes Data
T22976 10009-10012 VBP denotes are
T22977 10013-10022 VBN denotes expressed
T22978 10023-10027 JJ denotes mean
T22979 10028-10029 NN denotes ±
T22980 10030-10035 NNP denotes S.E.M
T22981 10035-10036 , denotes ,
T22982 10037-10040 IN denotes for
T22983 10041-10046 CD denotes three
T22984 10047-10058 JJ denotes independent
T22985 10059-10070 NNS denotes experiments
T22986 10070-10071 . denotes .
T4839 10074-10076 PRP denotes We
T4840 10077-10081 JJ denotes next
T4841 10082-10094 VBD denotes investigated
T4842 10095-10102 IN denotes whether
T4843 10103-10108 NNP denotes M-CSF
T4844 10109-10116 VBD denotes induced
T4845 10117-10122 JJ denotes NF-κB
T4846 10123-10131 NN denotes activity
T4847 10132-10134 IN denotes in
T4848 10135-10138 DT denotes the
T4849 10139-10144 NN denotes mouse
T4850 10145-10155 NN denotes macrophage
T4851 10156-10160 NN denotes cell
T4852 10161-10165 NN denotes line
T4853 10165-10166 , denotes ,
T4854 10167-10170 NNP denotes RAW
T4855 10171-10176 CD denotes 264.7
T4856 10176-10177 . denotes .
T4857 10178-10181 NNP denotes RAW
T4858 10182-10187 CD denotes 264.7
T4859 10188-10193 NNS denotes cells
T4860 10194-10198 VBD denotes were
T4861 10199-10210 VBN denotes transfected
T4862 10211-10215 IN denotes with
T4863 10216-10222 CC denotes either
T4864 10223-10226 DT denotes the
T4865 10227-10237 NNP denotes NF-κB-SEAP
T4866 10238-10246 NN denotes reporter
T4867 10247-10249 CC denotes or
T4868 10250-10257 NN denotes control
T4869 10258-10267 JJ denotes pTAL-SEAP
T4870 10268-10277 VBP denotes construct
T4871 10277-10278 . denotes .
T4872 10279-10281 IN denotes As
T4873 10282-10287 VBN denotes shown
T4874 10288-10290 IN denotes in
T4875 10291-10297 NN denotes Figure
T4876 10298-10300 CD denotes 1C
T4877 10300-10301 , denotes ,
T4878 10302-10307 NNP denotes M-CSF
T4879 10308-10317 NN denotes treatment
T4880 10318-10320 IN denotes of
T4881 10321-10324 NNP denotes RAW
T4882 10325-10330 CD denotes 264.7
T4883 10331-10336 NNS denotes cells
T4884 10337-10346 VBD denotes increased
T4885 10347-10352 JJ denotes NF-κB
T4886 10353-10361 NN denotes reporter
T4887 10362-10370 NN denotes activity
T4888 10371-10373 IN denotes by
T4889 10374-10382 CD denotes 2.5-fold
T4890 10383-10387 IN denotes over
T4891 10388-10392 DT denotes that
T4892 10393-10395 IN denotes of
T4893 10396-10407 JJ denotes non-treated
T4894 10408-10413 NNS denotes cells
T4895 10413-10414 . denotes .
T4896 10415-10423 RB denotes Together
T4897 10423-10424 , denotes ,
T4898 10425-10428 PRP$ denotes our
T4899 10429-10433 NNS denotes data
T4900 10434-10445 VBP denotes demonstrate
T4901 10446-10450 IN denotes that
T4902 10451-10456 NNP denotes M-CSF
T4903 10457-10464 VBD denotes induced
T4904 10465-10470 NNP denotes NF-κB
T4905 10471-10486 JJ denotes transcriptional
T4906 10487-10495 NN denotes activity
T4907 10496-10498 IN denotes in
T4908 10499-10510 NNS denotes macrophages
T4909 10510-10511 . denotes .
T5344 10513-10516 NNP denotes PKC
T5345 10517-10527 NNP denotes Inhibition
T5346 10528-10535 VBZ denotes Reduces
T5347 10536-10541 NNP denotes NF-κB
T5348 10542-10550 NNP denotes Activity
T5349 10551-10553 IN denotes in
T5350 10554-10559 NNP denotes Human
T5351 10560-10564 NNPS denotes MDMs
T5352 10565-10568 CC denotes and
T5353 10569-10572 NNP denotes RAW
T5354 10573-10578 CD denotes 264.7
T5355 10579-10584 NNP denotes Cells
T5356 10585-10590 IN denotes Since
T5357 10591-10596 NNP denotes NF-κB
T5358 10597-10599 VBZ denotes is
T5359 10600-10609 VBN denotes activated
T5360 10610-10612 IN denotes by
T5361 10613-10616 NNP denotes PKC
T5362 10617-10619 IN denotes in
T5363 10620-10627 JJ denotes several
T5364 10628-10632 NN denotes cell
T5365 10633-10638 NNS denotes types
T5366 10639-10640 NNP denotes [
T5367 10640-10642 CD denotes 36
T5368 10642-10643 NNP denotes ]
T5369 10643-10644 , denotes ,
T5370 10645-10647 PRP denotes we
T5371 10648-10652 JJ denotes next
T5372 10653-10663 VBN denotes determined
T5373 10664-10666 IN denotes if
T5374 10667-10680 JJ denotes M-CSF-induced
T5375 10681-10686 NN denotes NF-κB
T5376 10687-10702 JJ denotes transcriptional
T5377 10703-10711 NN denotes activity
T5378 10712-10715 VBD denotes was
T5379 10716-10725 JJ denotes dependent
T5380 10726-10728 IN denotes on
T5381 10729-10732 NNP denotes PKC
T5382 10733-10743 NN denotes activation
T5383 10744-10747 CC denotes and
T5384 10748-10750 IN denotes if
T5385 10751-10758 NN denotes calcium
T5386 10758-10759 , denotes ,
T5387 10760-10761 DT denotes a
T5388 10762-10771 NN denotes co-factor
T5389 10772-10775 IN denotes for
T5390 10776-10788 JJ denotes conventional
T5391 10789-10792 NNP denotes PKC
T5392 10793-10800 NN denotes isoform
T5393 10801-10811 NN denotes activation
T5394 10811-10812 , denotes ,
T5395 10813-10816 VBD denotes was
T5396 10817-10826 JJ denotes important
T5397 10826-10827 . denotes .
T5398 10828-10830 IN denotes In
T5399 10831-10839 NN denotes addition
T5400 10839-10840 , denotes ,
T5401 10841-10848 IN denotes because
T5402 10849-10851 IN denotes of
T5403 10852-10855 DT denotes the
T5404 10856-10862 NN denotes number
T5405 10863-10865 IN denotes of
T5406 10866-10878 JJ denotes conventional
T5407 10879-10883 NNS denotes PKCs
T5408 10884-10892 VBG denotes existing
T5409 10893-10899 IN denotes within
T5410 10900-10911 NN denotes mononuclear
T5411 10912-10917 NNS denotes cells
T5412 10917-10918 , denotes ,
T5413 10919-10934 JJ denotes pharmacological
T5414 10935-10945 NNS denotes inhibitors
T5415 10946-10948 IN denotes of
T5416 10949-10952 NNP denotes PKC
T5417 10953-10959 NN denotes family
T5418 10960-10970 NN denotes activation
T5419 10971-10974 VBD denotes was
T5420 10975-10979 VBN denotes used
T5421 10980-10982 TO denotes to
T5422 10983-10992 VB denotes determine
T5423 10993-10996 DT denotes the
T5424 10997-11009 NN denotes relationship
T5425 11010-11012 IN denotes of
T5426 11013-11016 NNP denotes PKC
T5427 11017-11027 NN denotes activation
T5428 11028-11030 TO denotes to
T5429 11031-11044 JJ denotes M-CSF-induced
T5430 11045-11053 JJ denotes cellular
T5431 11054-11062 NN denotes survival
T5432 11062-11063 . denotes .
T5433 11064-11068 NNS denotes MDMs
T5434 11069-11073 VBD denotes were
T5435 11074-11085 VBN denotes transfected
T5436 11086-11090 IN denotes with
T5437 11091-11094 DT denotes the
T5438 11095-11106 JJ denotes pNF-κB-SEAP
T5439 11107-11115 NN denotes reporter
T5440 11116-11119 CC denotes and
T5441 11120-11124 RB denotes then
T5442 11125-11132 VBN denotes treated
T5443 11133-11137 IN denotes with
T5444 11138-11141 DT denotes the
T5445 11142-11149 JJ denotes general
T5446 11150-11153 NNP denotes PKC
T5447 11154-11163 NN denotes inhibitor
T5448 11163-11164 , denotes ,
T5449 11165-11175 NN denotes Ro-31-8220
T5450 11175-11176 : denotes ;
T5451 11177-11180 DT denotes the
T5452 11181-11193 JJ denotes conventional
T5453 11194-11198 NNP denotes PKCα
T5454 11198-11199 NN denotes /
T5455 11199-11200 NN denotes β
T5456 11201-11210 NN denotes inhibitor
T5457 11211-11218 NNP denotes Gö-6976
T5458 11218-11219 : denotes ;
T5459 11220-11222 CC denotes or
T5460 11223-11226 DT denotes the
T5461 11227-11240 JJ denotes intracellular
T5462 11241-11248 NN denotes calcium
T5463 11249-11256 NN denotes blocker
T5464 11257-11265 NNP denotes BAPTA/AM
T5465 11265-11266 . denotes .
T5466 11267-11277 JJ denotes Ro-31-8220
T5467 11278-11291 RB denotes significantly
T5468 11292-11302 VBN denotes suppressed
T5469 11303-11316 JJ denotes M-CSF-induced
T5470 11317-11322 NN denotes NF-κB
T5471 11323-11331 NN denotes activity
T5472 11332-11340 VBN denotes compared
T5473 11341-11343 TO denotes to
T5474 11344-11349 NNS denotes cells
T5475 11350-11357 VBN denotes treated
T5476 11358-11362 IN denotes with
T5477 11363-11368 NNP denotes M-CSF
T5478 11369-11374 RB denotes alone
T5479 11375-11376 -LRB- denotes (
T5480 11376-11382 NN denotes Figure
T5481 11383-11385 NN denotes 2A
T5482 11385-11386 -RRB- denotes )
T5483 11386-11387 . denotes .
T5484 11388-11390 IN denotes In
T5485 11391-11399 NN denotes addition
T5486 11399-11400 , denotes ,
T5487 11401-11408 NNP denotes Gö-6976
T5488 11409-11412 CC denotes and
T5489 11413-11421 NNP denotes BAPTA/AM
T5490 11422-11426 RB denotes also
T5491 11427-11434 VBD denotes blocked
T5492 11435-11440 JJ denotes NF-κB
T5493 11441-11449 NN denotes activity
T5494 11450-11452 IN denotes in
T5495 11453-11466 JJ denotes M-CSF-treated
T5496 11467-11471 NNS denotes MDMs
T5497 11471-11472 . denotes .
T5498 11473-11479 NNP denotes Trypan
T5499 11480-11484 JJ denotes blue
T5500 11485-11494 NN denotes exclusion
T5501 11495-11503 NN denotes analysis
T5502 11504-11507 VBD denotes did
T5503 11508-11511 RB denotes not
T5504 11512-11520 VB denotes indicate
T5505 11521-11525 NN denotes cell
T5506 11526-11531 NN denotes death
T5507 11532-11542 VBG denotes suggesting
T5508 11543-11547 IN denotes that
T5509 11548-11552 DT denotes this
T5510 11553-11564 NN denotes suppression
T5511 11565-11568 VBD denotes was
T5512 11569-11572 RB denotes not
T5513 11573-11576 JJ denotes due
T5514 11577-11579 TO denotes to
T5515 11580-11592 JJ denotes non-specific
T5516 11593-11601 NN denotes toxicity
T5517 11601-11602 . denotes .
T5518 11603-11608 DT denotes These
T5519 11609-11613 NNS denotes data
T5520 11614-11622 VBP denotes indicate
T5521 11623-11627 IN denotes that
T5522 11628-11633 NNP denotes M-CSF
T5523 11634-11642 VBD denotes mediated
T5524 11643-11648 NNP denotes NF-κB
T5525 11649-11659 NN denotes activation
T5526 11660-11667 IN denotes through
T5527 11668-11685 JJ denotes calcium-dependent
T5528 11686-11698 JJ denotes conventional
T5529 11699-11702 NNP denotes PKC
T5530 11703-11713 NN denotes activation
T5531 11713-11714 . denotes .
T23222 11759-11769 NNP denotes Inhibition
T23223 11770-11772 IN denotes of
T23224 11773-11776 NNP denotes PKC
T23225 11777-11784 VBZ denotes reduces
T23226 11785-11790 JJ denotes NF-κB
T23227 11791-11799 NN denotes activity
T23228 11800-11802 IN denotes in
T23229 11803-11808 NNP denotes M-CSF
T23230 11809-11819 VBD denotes stimulated
T23231 11820-11831 NNS denotes macrophages
T23232 11831-11832 . denotes .
T23233 11833-11834 -LRB- denotes (
T23234 11834-11835 NNP denotes A
T23235 11835-11836 -RRB- denotes )
T23236 11837-11842 NNP denotes Human
T23237 11843-11847 NNS denotes MDMs
T23238 11848-11859 VBN denotes transfected
T23239 11860-11864 IN denotes with
T23240 11865-11876 JJ denotes pNF-κB-SEAP
T23241 11877-11881 VBD denotes were
T23242 11882-11895 VBN denotes pre-incubated
T23243 11896-11900 IN denotes with
T23244 11901-11911 NNS denotes inhibitors
T23245 11911-11912 : denotes ;
T23246 11913-11923 NNP denotes Ro-31-8220
T23247 11923-11924 , denotes ,
T23248 11925-11932 NNP denotes Gö-6976
T23249 11932-11933 , denotes ,
T23250 11934-11936 CC denotes or
T23251 11937-11945 NNP denotes BAPTA/AM
T23252 11946-11949 IN denotes for
T23253 11950-11952 CD denotes 30
T23254 11953-11960 NNS denotes minutes
T23255 11961-11966 RB denotes prior
T23256 11967-11969 TO denotes to
T23257 11970-11971 CD denotes 6
T23258 11972-11977 NNS denotes hours
T23259 11978-11983 JJ denotes M-CSF
T23260 11984-11995 NN denotes stimulation
T23261 11995-11996 . denotes .
T23262 11997-11998 -LRB- denotes (
T23263 11998-11999 NNP denotes B
T23264 11999-12000 -RRB- denotes )
T23265 12001-12004 NNP denotes RAW
T23266 12005-12010 CD denotes 264.7
T23267 12011-12016 NNS denotes cells
T23268 12017-12028 VBN denotes transfected
T23269 12029-12033 IN denotes with
T23270 12034-12037 DT denotes the
T23271 12038-12049 NNP denotes pNF-κB-SEAP
T23272 12050-12059 VBP denotes construct
T23273 12060-12064 VBD denotes were
T23274 12065-12078 VBN denotes pre-incubated
T23275 12079-12083 IN denotes with
T23276 12084-12094 NNS denotes inhibitors
T23277 12095-12098 IN denotes for
T23278 12099-12101 CD denotes 30
T23279 12102-12109 NNS denotes minutes
T23280 12110-12115 RB denotes prior
T23281 12116-12118 TO denotes to
T23282 12119-12120 CD denotes 2
T23283 12121-12126 NNS denotes hours
T23284 12127-12129 IN denotes of
T23285 12130-12141 NN denotes stimulation
T23286 12142-12146 IN denotes with
T23287 12147-12152 NN denotes mouse
T23288 12153-12164 JJ denotes recombinant
T23289 12165-12170 NNP denotes M-CSF
T23290 12170-12171 . denotes .
T23291 12172-12175 DT denotes The
T23292 12176-12181 JJ denotes NF-κB
T23293 12182-12190 NN denotes activity
T23294 12191-12194 VBD denotes was
T23295 12195-12203 VBN denotes analyzed
T23296 12204-12206 IN denotes by
T23297 12207-12216 VBG denotes measuring
T23298 12217-12221 NNP denotes SEAP
T23299 12222-12232 NN denotes production
T23300 12233-12235 IN denotes in
T23301 12236-12239 DT denotes the
T23302 12240-12246 NN denotes medium
T23303 12247-12250 CC denotes and
T23304 12251-12255 NNS denotes data
T23305 12256-12259 VBP denotes are
T23306 12260-12269 VBN denotes expressed
T23307 12270-12272 IN denotes as
T23308 12273-12277 VBP denotes fold
T23309 12278-12286 NN denotes increase
T23310 12287-12289 IN denotes of
T23311 12290-12294 NNP denotes SEAP
T23312 12295-12303 NN denotes activity
T23313 12304-12308 IN denotes over
T23314 12309-12313 DT denotes that
T23315 12314-12316 IN denotes in
T23316 12317-12326 JJ denotes pTAL-SEAP
T23317 12327-12338 VBN denotes transfected
T23318 12339-12346 VBG denotes resting
T23319 12347-12352 NNS denotes cells
T23320 12352-12353 . denotes .
T23321 12354-12357 DT denotes The
T23322 12358-12363 NN denotes graph
T23323 12364-12374 VBZ denotes represents
T23324 12375-12379 JJ denotes mean
T23325 12380-12381 NN denotes ±
T23326 12382-12387 NNP denotes S.E.M
T23327 12388-12391 IN denotes for
T23328 12392-12397 CD denotes three
T23329 12398-12409 JJ denotes independent
T23330 12410-12421 NNS denotes experiments
T23331 12421-12422 . denotes .
T23332 12423-12424 SYM denotes *
T23333 12424-12427 DT denotes The
T23334 12428-12436 NNS denotes p-values
T23335 12437-12439 IN denotes of
T23336 12440-12445 NNS denotes cells
T23337 12446-12453 VBN denotes treated
T23338 12454-12458 IN denotes with
T23339 12459-12475 JJ denotes inhibitors/M-CSF
T23340 12476-12484 VBN denotes compared
T23341 12485-12487 TO denotes to
T23342 12488-12501 NNP denotes vehicle/M-CSF
T23343 12502-12506 VBD denotes were
T23344 12507-12508 CD denotes ≤
T23345 12508-12512 CD denotes 0.05
T23346 12512-12513 . denotes .
T5532 12516-12518 PRP denotes We
T5533 12519-12523 JJ denotes next
T5534 12524-12536 VBD denotes investigated
T5535 12537-12544 IN denotes whether
T5536 12545-12548 NNP denotes PKC
T5537 12549-12559 NN denotes inhibition
T5538 12560-12568 VBD denotes affected
T5539 12569-12574 JJ denotes NF-κB
T5540 12575-12583 NN denotes activity
T5541 12584-12586 IN denotes in
T5542 12587-12590 DT denotes the
T5543 12591-12596 NN denotes mouse
T5544 12597-12607 NN denotes macrophage
T5545 12608-12612 NN denotes cell
T5546 12613-12617 NN denotes line
T5547 12617-12618 , denotes ,
T5548 12619-12622 NNP denotes RAW
T5549 12623-12628 CD denotes 264.7
T5550 12628-12629 . denotes .
T5551 12630-12637 JJ denotes Similar
T5552 12638-12640 TO denotes to
T5553 12641-12645 NNS denotes MDMs
T5554 12645-12646 , denotes ,
T5555 12647-12650 NNP denotes PKC
T5556 12651-12661 NNS denotes inhibitors
T5557 12662-12672 NNP denotes Ro-31-8220
T5558 12672-12673 , denotes ,
T5559 12674-12681 NNP denotes Gö-6976
T5560 12682-12685 CC denotes and
T5561 12686-12694 NNP denotes BAPTA/AM
T5562 12695-12702 VBD denotes reduced
T5563 12703-12716 JJ denotes M-CSF-induced
T5564 12717-12722 NN denotes NF-κB
T5565 12723-12731 NN denotes activity
T5566 12732-12734 IN denotes in
T5567 12735-12736 DT denotes a
T5568 12737-12751 JJ denotes dose-dependent
T5569 12752-12758 NN denotes manner
T5570 12759-12761 IN denotes in
T5571 12762-12765 NNP denotes RAW
T5572 12766-12771 CD denotes 264.7
T5573 12772-12783 NNS denotes macrophages
T5574 12784-12785 -LRB- denotes (
T5575 12785-12791 NN denotes Figure
T5576 12792-12794 NN denotes 2B
T5577 12794-12795 -RRB- denotes )
T5578 12795-12796 . denotes .
T5579 12797-12799 TO denotes To
T5580 12800-12806 VB denotes ensure
T5581 12807-12811 IN denotes that
T5582 12812-12815 NNP denotes PKC
T5583 12816-12828 RB denotes specifically
T5584 12829-12838 VBD denotes regulated
T5585 12839-12844 NNP denotes NF-κB
T5586 12845-12848 CD denotes p65
T5587 12849-12852 CC denotes and
T5588 12853-12856 RB denotes not
T5589 12857-12860 DT denotes the
T5590 12861-12868 RB denotes closely
T5591 12869-12876 JJ denotes related
T5592 12877-12883 NN denotes family
T5593 12884-12890 NN denotes member
T5594 12890-12891 , denotes ,
T5595 12892-12897 NNP denotes c-Rel
T5596 12897-12898 , denotes ,
T5597 12899-12901 PRP denotes we
T5598 12902-12916 VBD denotes co-transfected
T5599 12917-12922 NNP denotes c-Rel
T5600 12923-12926 CC denotes and
T5601 12927-12937 NNP denotes NF-κB-SEAP
T5602 12938-12948 VBZ denotes constructs
T5603 12949-12953 IN denotes into
T5604 12954-12957 DT denotes the
T5605 12958-12961 NNP denotes Raw
T5606 12962-12967 CD denotes 264.7
T5607 12968-12972 NN denotes cell
T5608 12973-12977 NN denotes line
T5609 12978-12981 CC denotes and
T5610 12982-12990 VBN denotes measured
T5611 12991-12996 JJ denotes NF-κB
T5612 12997-13005 NN denotes activity
T5613 13006-13008 IN denotes in
T5614 13009-13017 NN denotes response
T5615 13018-13020 TO denotes to
T5616 13021-13026 NNP denotes M-CSF
T5617 13026-13027 . denotes .
T5618 13028-13033 EX denotes There
T5619 13034-13037 VBD denotes was
T5620 13038-13040 DT denotes no
T5621 13041-13050 VBN denotes increased
T5622 13051-13056 NN denotes NF-κB
T5623 13057-13065 NN denotes activity
T5624 13066-13068 IN denotes in
T5625 13069-13074 NNS denotes cells
T5626 13075-13085 VBG denotes expressing
T5627 13086-13091 NNP denotes c-Rel
T5628 13092-13093 -LRB- denotes (
T5629 13093-13099 NNP denotes Figure
T5630 13100-13102 NNP denotes S1
T5631 13102-13103 -RRB- denotes )
T5632 13103-13104 . denotes .
T5633 13105-13110 DT denotes These
T5634 13111-13123 NNS denotes observations
T5635 13124-13132 VBP denotes indicate
T5636 13133-13137 IN denotes that
T5637 13138-13141 NNP denotes PKC
T5638 13142-13152 VBD denotes functioned
T5639 13153-13161 RB denotes upstream
T5640 13162-13164 IN denotes of
T5641 13165-13170 NNP denotes NF-κB
T5642 13171-13174 CD denotes p65
T5643 13175-13177 IN denotes in
T5644 13178-13182 NNS denotes MDMs
T5645 13183-13186 CC denotes and
T5646 13187-13190 NNP denotes RAW
T5647 13191-13196 CD denotes 264.7
T5648 13197-13202 NNS denotes cells
T5649 13202-13203 . denotes .
T6258 13205-13210 NN denotes NF-κB
T6259 13211-13214 CC denotes and
T6260 13215-13218 NNP denotes PKC
T6261 13218-13219 -LRB- denotes (
T6262 13219-13220 PRP denotes s
T6263 13220-13221 -RRB- denotes )
T6264 13222-13229 NNP denotes Mediate
T6265 13230-13235 NNP denotes Human
T6266 13236-13239 NNP denotes MDM
T6267 13240-13248 NNP denotes Survival
T6268 13249-13251 IN denotes in
T6269 13252-13260 NNP denotes Response
T6270 13261-13263 TO denotes to
T6271 13264-13269 NNP denotes M-CSF
T6272 13270-13281 NNP denotes Stimulation
T6273 13282-13287 IN denotes Since
T6274 13288-13291 NNP denotes PKC
T6275 13292-13295 CC denotes and
T6276 13296-13301 NNP denotes NF-κB
T6277 13302-13305 VBP denotes are
T6278 13306-13314 JJ denotes critical
T6279 13315-13317 IN denotes in
T6280 13318-13322 NN denotes cell
T6281 13323-13331 NN denotes survival
T6282 13332-13333 NNP denotes [
T6283 13333-13335 CD denotes 37
T6284 13335-13336 NNP denotes ]
T6285 13336-13337 , denotes ,
T6286 13338-13339 NNP denotes [
T6287 13339-13341 CD denotes 38
T6288 13341-13342 NNP denotes ]
T6289 13342-13343 , denotes ,
T6290 13344-13346 PRP denotes we
T6291 13347-13359 VBD denotes hypothesized
T6292 13360-13364 IN denotes that
T6293 13365-13370 NNP denotes M-CSF
T6294 13371-13379 VBD denotes promoted
T6295 13380-13384 NN denotes cell
T6296 13385-13393 NN denotes survival
T6297 13394-13401 IN denotes through
T6298 13402-13405 NNP denotes PKC
T6299 13406-13408 IN denotes in
T6300 13409-13414 JJ denotes human
T6301 13415-13419 NNS denotes MDMs
T6302 13419-13420 . denotes .
T6303 13421-13423 TO denotes To
T6304 13424-13430 VB denotes detect
T6305 13431-13440 NN denotes apoptosis
T6306 13440-13441 , denotes ,
T6307 13442-13445 DT denotes the
T6308 13446-13456 NN denotes expression
T6309 13457-13459 IN denotes of
T6310 13460-13467 JJ denotes cleaved
T6311 13468-13477 JJ denotes caspase-3
T6312 13477-13478 , denotes ,
T6313 13479-13480 DT denotes a
T6314 13481-13487 NN denotes marker
T6315 13488-13490 IN denotes of
T6316 13491-13500 NN denotes apoptosis
T6317 13500-13501 , denotes ,
T6318 13502-13505 VBD denotes was
T6319 13506-13514 VBN denotes analyzed
T6320 13515-13517 IN denotes in
T6321 13518-13521 DT denotes the
T6322 13522-13530 NN denotes presence
T6323 13531-13533 CC denotes or
T6324 13534-13541 NN denotes absence
T6325 13542-13544 IN denotes of
T6326 13545-13550 NNP denotes M-CSF
T6327 13551-13554 CC denotes and
T6328 13555-13558 NNP denotes PKC
T6329 13559-13569 NNS denotes inhibitors
T6330 13569-13570 . denotes .
T6331 13571-13573 IN denotes In
T6332 13574-13578 NNS denotes MDMs
T6333 13579-13589 VBN denotes pretreated
T6334 13590-13594 IN denotes with
T6335 13595-13598 NNP denotes PKC
T6336 13599-13609 NNS denotes inhibitors
T6337 13610-13611 -LRB- denotes (
T6338 13611-13621 NN denotes Ro-31-8220
T6339 13621-13622 , denotes ,
T6340 13623-13630 NNP denotes Gö-6976
T6341 13630-13631 , denotes ,
T6342 13632-13634 CC denotes or
T6343 13635-13643 NNP denotes BAPTA/AM
T6344 13643-13644 -RRB- denotes )
T6345 13645-13648 CC denotes and
T6346 13649-13659 VBN denotes stimulated
T6347 13660-13664 IN denotes with
T6348 13665-13670 NNP denotes M-CSF
T6349 13670-13671 , denotes ,
T6350 13672-13679 VBD denotes cleaved
T6351 13680-13689 JJ denotes caspase-3
T6352 13690-13693 VBD denotes was
T6353 13694-13702 VBN denotes elevated
T6354 13703-13705 TO denotes to
T6355 13706-13712 NNS denotes levels
T6356 13713-13715 IN denotes of
T6357 13716-13721 NNS denotes cells
T6358 13722-13729 VBN denotes treated
T6359 13730-13734 IN denotes with
T6360 13735-13742 NN denotes vehicle
T6361 13743-13748 RB denotes alone
T6362 13749-13750 -LRB- denotes (
T6363 13750-13756 NN denotes Figure
T6364 13757-13759 NN denotes 3A
T6365 13759-13760 -RRB- denotes )
T6366 13760-13761 . denotes .
T6367 13762-13764 IN denotes In
T6368 13765-13773 NN denotes contrast
T6369 13773-13774 , denotes ,
T6370 13775-13780 NNS denotes cells
T6371 13781-13790 VBN denotes incubated
T6372 13791-13795 IN denotes with
T6373 13796-13801 NNP denotes M-CSF
T6374 13802-13805 CC denotes and
T6375 13806-13813 NN denotes vehicle
T6376 13814-13817 VBD denotes had
T6377 13818-13822 RBR denotes less
T6378 13823-13830 JJ denotes cleaved
T6379 13831-13840 JJ denotes caspase-3
T6380 13841-13845 IN denotes than
T6381 13846-13851 NNS denotes cells
T6382 13852-13861 VBN denotes incubated
T6383 13862-13866 IN denotes with
T6384 13867-13874 NN denotes vehicle
T6385 13875-13880 RB denotes alone
T6386 13881-13883 CC denotes or
T6387 13884-13889 RB denotes M-CSF
T6388 13890-13894 IN denotes with
T6389 13895-13898 NNP denotes PKC
T6390 13899-13909 NNS denotes inhibitors
T6391 13909-13910 . denotes .
T6392 13911-13916 DT denotes These
T6393 13917-13921 NNS denotes data
T6394 13922-13931 VBD denotes supported
T6395 13932-13935 DT denotes the
T6396 13936-13946 NN denotes hypothesis
T6397 13947-13951 WDT denotes that
T6398 13952-13965 VBD denotes M-CSF-induced
T6399 13966-13971 JJ denotes NF-κB
T6400 13972-13980 NN denotes activity
T6401 13981-13984 VBD denotes was
T6402 13985-13994 VBN denotes regulated
T6403 13995-13997 IN denotes by
T6404 13998-14010 JJ denotes conventional
T6405 14011-14015 NNS denotes PKCs
T6406 14015-14016 , denotes ,
T6407 14017-14020 RB denotes not
T6408 14021-14026 JJ denotes novel
T6409 14027-14031 NNS denotes PKCs
T6410 14031-14032 . denotes .
T23560 14077-14087 NNP denotes Inhibition
T23561 14088-14090 IN denotes of
T23562 14091-14094 NNP denotes PKC
T23563 14095-14097 CC denotes or
T23564 14098-14103 NNP denotes NF-κB
T23565 14104-14111 VBZ denotes induces
T23566 14112-14121 NN denotes apoptosis
T23567 14122-14124 IN denotes in
T23568 14125-14129 NNS denotes MDMs
T23569 14129-14130 . denotes .
T23570 14131-14132 -LRB- denotes (
T23571 14132-14133 NNP denotes A
T23572 14133-14134 -RRB- denotes )
T23573 14135-14139 NNS denotes MDMs
T23574 14140-14144 VBD denotes were
T23575 14145-14158 VBN denotes pre-incubated
T23576 14159-14161 IN denotes in
T23577 14162-14166 NNP denotes RPMI
T23578 14167-14173 NN denotes medium
T23579 14174-14184 VBG denotes containing
T23580 14185-14195 NNS denotes inhibitors
T23581 14196-14197 -LRB- denotes (
T23582 14197-14207 NN denotes Ro-31-8220
T23583 14207-14208 : denotes :
T23584 14209-14210 CD denotes 5
T23585 14211-14213 NNP denotes µM
T23586 14213-14214 , denotes ,
T23587 14215-14222 NNP denotes Gö-6976
T23588 14222-14223 : denotes :
T23589 14224-14225 CD denotes 5
T23590 14226-14228 NNP denotes µM
T23591 14228-14229 , denotes ,
T23592 14230-14238 NNP denotes BAPTA/AM
T23593 14238-14239 : denotes :
T23594 14240-14243 CD denotes 2.5
T23595 14244-14246 NNP denotes µM
T23596 14246-14247 -RRB- denotes )
T23597 14248-14251 IN denotes for
T23598 14252-14254 CD denotes 30
T23599 14255-14262 NNS denotes minutes
T23600 14263-14268 RB denotes prior
T23601 14269-14271 TO denotes to
T23602 14272-14275 DT denotes the
T23603 14276-14284 NN denotes addition
T23604 14285-14287 IN denotes of
T23605 14288-14293 NNP denotes M-CSF
T23606 14293-14294 . denotes .
T23607 14295-14297 IN denotes As
T23608 14298-14299 DT denotes a
T23609 14300-14307 NN denotes control
T23610 14307-14308 , denotes ,
T23611 14309-14318 JJ denotes untreated
T23612 14319-14324 NNS denotes cells
T23613 14325-14329 VBD denotes were
T23614 14330-14339 VBN denotes incubated
T23615 14340-14344 IN denotes with
T23616 14345-14353 NN denotes dimethyl
T23617 14354-14363 NN denotes sulfoxide
T23618 14364-14365 -LRB- denotes (
T23619 14365-14369 NNP denotes DMSO
T23620 14369-14370 -RRB- denotes )
T23621 14370-14371 . denotes .
T23622 14372-14376 NN denotes Cell
T23623 14377-14384 NNS denotes lysates
T23624 14385-14389 VBD denotes were
T23625 14390-14398 VBN denotes resolved
T23626 14399-14401 IN denotes by
T23627 14402-14410 NNP denotes SDS-PAGE
T23628 14411-14414 CC denotes and
T23629 14415-14428 VBN denotes immunoblotted
T23630 14429-14433 IN denotes with
T23631 14434-14442 NN denotes antibody
T23632 14443-14454 VBG denotes recognizing
T23633 14455-14458 DT denotes the
T23634 14459-14465 JJ denotes active
T23635 14466-14473 JJ denotes cleaved
T23636 14474-14478 NN denotes form
T23637 14479-14481 IN denotes of
T23638 14482-14491 JJ denotes caspase-3
T23639 14491-14492 . denotes .
T23640 14493-14496 DT denotes The
T23641 14497-14502 NNS denotes blots
T23642 14503-14507 VBD denotes were
T23643 14508-14517 VBN denotes reblotted
T23644 14518-14522 IN denotes with
T23645 14523-14530 NN denotes β-actin
T23646 14531-14535 IN denotes that
T23647 14536-14542 VBD denotes served
T23648 14543-14545 IN denotes as
T23649 14546-14547 DT denotes a
T23650 14548-14555 VBG denotes loading
T23651 14556-14563 NN denotes control
T23652 14563-14564 . denotes .
T23653 14565-14568 DT denotes The
T23654 14569-14574 NN denotes ratio
T23655 14575-14577 IN denotes of
T23656 14578-14584 JJ denotes active
T23657 14585-14594 JJ denotes caspase-3
T23658 14595-14600 NNS denotes bands
T23659 14601-14602 -LRB- denotes (
T23660 14602-14604 CD denotes 17
T23661 14605-14607 NN denotes kD
T23662 14608-14611 CC denotes and
T23663 14612-14614 CD denotes 19
T23664 14615-14617 NN denotes kD
T23665 14617-14618 -RRB- denotes )
T23666 14619-14621 TO denotes to
T23667 14622-14629 VB denotes β-actin
T23668 14630-14637 NN denotes control
T23669 14638-14641 VBD denotes was
T23670 14642-14652 VBN denotes determined
T23671 14653-14655 IN denotes by
T23672 14656-14668 NN denotes densitometry
T23673 14669-14677 NN denotes analysis
T23674 14678-14679 -LRB- denotes (
T23675 14679-14685 JJ denotes bottom
T23676 14686-14691 NN denotes panel
T23677 14691-14692 -RRB- denotes )
T23678 14692-14693 . denotes .
T23679 14694-14698 NNP denotes Data
T23680 14699-14709 VBZ denotes represents
T23681 14710-14713 DT denotes the
T23682 14714-14718 JJ denotes mean
T23683 14719-14720 NN denotes ±
T23684 14721-14726 NNP denotes S.E.M
T23685 14727-14731 IN denotes from
T23686 14732-14735 CD denotes two
T23687 14736-14747 JJ denotes independent
T23688 14748-14754 NNS denotes donors
T23689 14754-14755 . denotes .
T23690 14756-14757 -LRB- denotes (
T23691 14757-14758 NNP denotes B
T23692 14758-14759 -RRB- denotes )
T23693 14760-14769 NNP denotes Apoptosis
T23694 14770-14772 IN denotes of
T23695 14773-14776 DT denotes the
T23696 14777-14784 VBN denotes treated
T23697 14785-14789 NNS denotes MDMs
T23698 14790-14793 VBD denotes was
T23699 14794-14798 RB denotes also
T23700 14799-14807 VBN denotes measured
T23701 14808-14810 IN denotes by
T23702 14811-14818 NNP denotes Annexin
T23703 14819-14825 NNP denotes V-FITC
T23704 14826-14829 CC denotes and
T23705 14830-14839 NN denotes propidium
T23706 14840-14846 NN denotes iodine
T23707 14847-14848 -LRB- denotes (
T23708 14848-14850 NNP denotes PI
T23709 14850-14851 -RRB- denotes )
T23710 14852-14860 VBG denotes staining
T23711 14861-14864 CC denotes and
T23712 14865-14873 VBN denotes analyzed
T23713 14874-14876 IN denotes by
T23714 14877-14881 NN denotes flow
T23715 14882-14891 NN denotes cytometry
T23716 14891-14892 . denotes .
T23717 14893-14896 DT denotes The
T23718 14897-14907 NN denotes percentage
T23719 14908-14910 IN denotes of
T23720 14911-14920 VBG denotes surviving
T23721 14921-14926 NNS denotes cells
T23722 14927-14928 -LRB- denotes (
T23723 14928-14935 NNP denotes Annexin
T23724 14936-14940 NNP denotes V/PI
T23725 14941-14949 JJ denotes negative
T23726 14949-14950 -RRB- denotes )
T23727 14951-14954 IN denotes for
T23728 14955-14958 DT denotes the
T23729 14959-14964 NNS denotes cells
T23730 14965-14972 VBN denotes treated
T23731 14973-14977 IN denotes with
T23732 14978-14991 NNP denotes vehicle/M-CSF
T23733 14992-14995 VBD denotes was
T23734 14996-15007 RB denotes arbitrarily
T23735 15008-15011 VBN denotes set
T23736 15012-15014 IN denotes as
T23737 15015-15018 CD denotes 100
T23738 15018-15019 . denotes .
T23739 15020-15024 NNP denotes Data
T23740 15025-15030 VBN denotes shown
T23741 15031-15040 VBP denotes represent
T23742 15041-15044 DT denotes the
T23743 15045-15049 JJ denotes mean
T23744 15050-15051 NN denotes ±
T23745 15052-15057 NNP denotes S.E.M
T23746 15058-15062 IN denotes from
T23747 15063-15068 CD denotes three
T23748 15069-15080 JJ denotes independent
T23749 15081-15092 NNS denotes experiments
T23750 15092-15093 . denotes .
T23751 15094-15095 SYM denotes *
T23752 15095-15098 DT denotes The
T23753 15099-15107 NNS denotes p-values
T23754 15108-15110 IN denotes of
T23755 15111-15127 JJ denotes inhibitors/M-CSF
T23756 15128-15136 VBN denotes compared
T23757 15137-15139 TO denotes to
T23758 15140-15153 NNP denotes vehicle/M-CSF
T23759 15154-15158 VBD denotes were
T23760 15159-15160 CD denotes ≤
T23761 15160-15164 CD denotes 0.05
T23762 15164-15165 . denotes .
T6411 15168-15170 TO denotes To
T6412 15171-15178 VB denotes confirm
T6413 15179-15183 DT denotes that
T6414 15184-15187 NNP denotes PKC
T6415 15188-15198 NN denotes inhibition
T6416 15199-15208 VBD denotes decreased
T6417 15209-15213 NN denotes cell
T6418 15214-15222 NN denotes survival
T6419 15222-15223 , denotes ,
T6420 15224-15229 NNS denotes cells
T6421 15230-15234 VBD denotes were
T6422 15235-15242 VBN denotes treated
T6423 15243-15247 IN denotes with
T6424 15248-15253 NNP denotes M-CSF
T6425 15254-15256 IN denotes in
T6426 15257-15260 DT denotes the
T6427 15261-15268 NN denotes absence
T6428 15269-15271 CC denotes or
T6429 15272-15280 NN denotes presence
T6430 15281-15283 IN denotes of
T6431 15284-15287 NNP denotes PKC
T6432 15288-15298 NNS denotes inhibitors
T6433 15299-15302 CC denotes and
T6434 15303-15307 RB denotes then
T6435 15308-15312 RB denotes also
T6436 15313-15321 VBD denotes examined
T6437 15322-15325 IN denotes for
T6438 15326-15335 NN denotes apoptosis
T6439 15336-15338 IN denotes by
T6440 15339-15346 NNP denotes Annexin
T6441 15347-15351 NNP denotes V/PI
T6442 15352-15360 VBG denotes staining
T6443 15360-15361 . denotes .
T6444 15362-15365 NNP denotes PKC
T6445 15366-15376 NNS denotes inhibitors
T6446 15377-15379 IN denotes in
T6447 15380-15383 DT denotes the
T6448 15384-15392 NN denotes presence
T6449 15393-15395 IN denotes of
T6450 15396-15401 NNP denotes M-CSF
T6451 15402-15409 VBD denotes reduced
T6452 15410-15413 DT denotes the
T6453 15414-15420 NN denotes number
T6454 15421-15423 IN denotes of
T6455 15424-15431 NNP denotes Annexin
T6456 15432-15436 NNP denotes V/PI
T6457 15437-15445 JJ denotes negative
T6458 15446-15450 NNS denotes MDMs
T6459 15451-15459 VBN denotes compared
T6460 15460-15462 TO denotes to
T6461 15463-15468 NNS denotes cells
T6462 15469-15476 VBN denotes treated
T6463 15477-15481 IN denotes with
T6464 15482-15487 NNP denotes M-CSF
T6465 15488-15493 RB denotes alone
T6466 15494-15495 -LRB- denotes (
T6467 15495-15501 NN denotes Figure
T6468 15502-15504 NN denotes 3B
T6469 15504-15505 -RRB- denotes )
T6470 15505-15506 . denotes .
T6471 15507-15512 DT denotes These
T6472 15513-15525 NNS denotes observations
T6473 15526-15533 RBR denotes further
T6474 15534-15543 VBD denotes suggested
T6475 15544-15548 IN denotes that
T6476 15549-15552 NNP denotes MDM
T6477 15553-15561 NN denotes survival
T6478 15562-15564 VBZ denotes is
T6479 15565-15573 VBN denotes promoted
T6480 15574-15576 IN denotes by
T6481 15577-15582 NNP denotes M-CSF
T6482 15583-15586 CC denotes and
T6483 15587-15597 RB denotes critically
T6484 15598-15606 VBZ denotes involves
T6485 15607-15619 JJ denotes conventional
T6486 15620-15624 NNS denotes PKCs
T6487 15625-15628 CC denotes and
T6488 15629-15634 JJ denotes NF-κB
T6489 15635-15643 NN denotes activity
T6490 15643-15644 . denotes .
T7014 15646-15659 NNS denotes M-CSF-Induces
T7015 15660-15664 NNP denotes PKCα
T7016 15665-15671 NNP denotes Kinase
T7017 15672-15680 NNP denotes Activity
T7018 15681-15686 IN denotes Since
T7019 15687-15690 PRP$ denotes our
T7020 15691-15695 NNS denotes data
T7021 15696-15705 VBD denotes suggested
T7022 15706-15710 IN denotes that
T7023 15711-15723 JJ denotes conventional
T7024 15724-15728 NNS denotes PKCs
T7025 15729-15733 VBD denotes were
T7026 15734-15742 VBN denotes involved
T7027 15743-15745 IN denotes in
T7028 15746-15756 VBG denotes activating
T7029 15757-15762 NN denotes NF-κB
T7030 15763-15765 IN denotes in
T7031 15766-15774 NN denotes response
T7032 15775-15777 TO denotes to
T7033 15778-15783 NNP denotes M-CSF
T7034 15784-15786 IN denotes in
T7035 15787-15794 JJ denotes primary
T7036 15795-15800 JJ denotes human
T7037 15801-15812 NNS denotes macrophages
T7038 15812-15813 , denotes ,
T7039 15814-15816 PRP denotes we
T7040 15817-15821 JJ denotes next
T7041 15822-15834 VBD denotes investigated
T7042 15835-15842 IN denotes whether
T7043 15843-15846 DT denotes the
T7044 15847-15859 JJ denotes conventional
T7045 15860-15863 NNP denotes PKC
T7046 15863-15864 , denotes ,
T7047 15865-15869 NNP denotes PKCα
T7048 15870-15873 VBD denotes was
T7049 15874-15875 DT denotes a
T7050 15876-15886 JJ denotes downstream
T7051 15887-15893 NN denotes target
T7052 15894-15896 IN denotes of
T7053 15897-15902 NNP denotes M-CSF
T7054 15903-15905 IN denotes in
T7055 15906-15911 JJ denotes human
T7056 15912-15916 NNS denotes MDMs
T7057 15916-15917 . denotes .
T7058 15918-15922 NNP denotes PKCα
T7059 15923-15926 VBD denotes was
T7060 15927-15945 VBN denotes immunoprecipitated
T7061 15946-15950 IN denotes from
T7062 15951-15956 JJ denotes human
T7063 15957-15961 NNS denotes MDMs
T7064 15962-15964 IN denotes at
T7065 15965-15968 DT denotes the
T7066 15969-15978 JJ denotes indicated
T7067 15979-15983 NN denotes time
T7068 15984-15990 NNS denotes points
T7069 15991-15992 -LRB- denotes (
T7070 15992-15998 NN denotes Figure
T7071 15999-16000 CD denotes 4
T7072 16000-16001 -RRB- denotes )
T7073 16001-16002 , denotes ,
T7074 16003-16006 CC denotes and
T7075 16007-16011 RB denotes then
T7076 16012-16013 DT denotes a
T7077 16014-16017 NNP denotes PKC
T7078 16018-16024 NN denotes kinase
T7079 16025-16030 NN denotes assay
T7080 16031-16034 VBD denotes was
T7081 16035-16044 VBN denotes performed
T7082 16045-16050 VBG denotes using
T7083 16051-16052 DT denotes a
T7084 16053-16071 JJ denotes fluorescein-tagged
T7085 16072-16079 NN denotes peptide
T7086 16080-16082 IN denotes as
T7087 16083-16084 DT denotes a
T7088 16085-16094 NN denotes substrate
T7089 16094-16095 . denotes .
T7090 16096-16098 IN denotes As
T7091 16099-16104 VBN denotes shown
T7092 16105-16107 IN denotes in
T7093 16108-16114 NN denotes Figure
T7094 16115-16116 CD denotes 4
T7095 16117-16118 -LRB- denotes (
T7096 16118-16123 JJ denotes upper
T7097 16124-16129 NN denotes panel
T7098 16129-16130 -RRB- denotes )
T7099 16130-16131 , denotes ,
T7100 16132-16135 DT denotes the
T7101 16136-16143 NN denotes peptide
T7102 16144-16153 NN denotes substrate
T7103 16154-16157 VBD denotes was
T7104 16158-16167 RB denotes maximally
T7105 16168-16182 VBN denotes phosphorylated
T7106 16183-16189 IN denotes within
T7107 16190-16192 CD denotes 10
T7108 16193-16200 NNS denotes minutes
T7109 16201-16203 IN denotes of
T7110 16204-16215 NN denotes stimulation
T7111 16216-16217 -LRB- denotes (
T7112 16217-16225 JJ denotes 1.8-fold
T7113 16226-16230 IN denotes over
T7114 16231-16238 VBG denotes resting
T7115 16239-16244 NNS denotes cells
T7116 16244-16245 , denotes ,
T7117 16246-16251 JJR denotes lower
T7118 16252-16257 NN denotes panel
T7119 16257-16258 -RRB- denotes )
T7120 16258-16259 , denotes ,
T7121 16260-16263 CC denotes and
T7122 16264-16268 RB denotes then
T7123 16269-16277 VBD denotes returned
T7124 16278-16280 TO denotes to
T7125 16281-16286 JJ denotes basal
T7126 16287-16293 NNS denotes levels
T7127 16294-16296 IN denotes by
T7128 16297-16299 CD denotes 15
T7129 16300-16307 NNS denotes minutes
T7130 16307-16308 . denotes .
T7131 16309-16313 DT denotes This
T7132 16314-16320 NN denotes effect
T7133 16321-16324 VBD denotes was
T7134 16325-16328 RB denotes not
T7135 16329-16333 VBN denotes seen
T7136 16334-16336 IN denotes in
T7137 16337-16353 JJ denotes M-CSF-stimulated
T7138 16354-16363 NNS denotes monocytes
T7139 16364-16368 WRB denotes when
T7140 16369-16377 JJ denotes isogenic
T7141 16378-16388 NNS denotes antibodies
T7142 16389-16390 -LRB- denotes (
T7143 16390-16393 NNP denotes IgG
T7144 16393-16394 -RRB- denotes )
T7145 16395-16399 VBD denotes were
T7146 16400-16404 VBN denotes used
T7147 16405-16408 IN denotes for
T7148 16409-16428 NN denotes immunoprecipitation
T7149 16428-16429 . denotes .
T7150 16430-16437 JJ denotes Western
T7151 16438-16443 NNS denotes blots
T7152 16444-16446 IN denotes of
T7153 16447-16456 JJ denotes identical
T7154 16457-16464 NNS denotes samples
T7155 16465-16470 VBG denotes using
T7156 16471-16473 DT denotes an
T7157 16474-16482 NN denotes antibody
T7158 16483-16494 VBG denotes recognizing
T7159 16495-16499 NNP denotes PKCα
T7160 16500-16512 VBD denotes demonstrated
T7161 16513-16517 IN denotes that
T7162 16518-16523 JJ denotes equal
T7163 16524-16531 NNS denotes amounts
T7164 16532-16534 IN denotes of
T7165 16535-16539 NNP denotes PKCα
T7166 16540-16544 VBD denotes were
T7167 16545-16552 VBN denotes assayed
T7168 16553-16554 -LRB- denotes (
T7169 16554-16560 NN denotes Figure
T7170 16561-16562 CD denotes 4
T7171 16562-16563 , denotes ,
T7172 16564-16570 JJ denotes middle
T7173 16571-16576 NN denotes panel
T7174 16576-16577 -RRB- denotes )
T7175 16577-16578 . denotes .
T24095 16623-16628 NNP denotes M-CSF
T24096 16629-16638 VBZ denotes activates
T24097 16639-16643 NNP denotes PKCα
T24098 16644-16646 IN denotes in
T24099 16647-16652 JJ denotes human
T24100 16653-16657 NNS denotes MDMs
T24101 16657-16658 . denotes .
T24102 16659-16663 NNS denotes MDMs
T24103 16664-16671 VBN denotes treated
T24104 16672-16676 IN denotes with
T24105 16677-16682 NNP denotes M-CSF
T24106 16683-16684 -LRB- denotes (
T24107 16684-16687 CD denotes 100
T24108 16688-16693 NN denotes ng/ml
T24109 16693-16694 -RRB- denotes )
T24110 16695-16698 IN denotes for
T24111 16699-16706 VBG denotes varying
T24112 16707-16714 NNS denotes amounts
T24113 16715-16717 IN denotes of
T24114 16718-16722 NN denotes time
T24115 16723-16727 VBD denotes were
T24116 16728-16733 VBN denotes lysed
T24117 16734-16737 CC denotes and
T24118 16738-16756 VBN denotes immunoprecipitated
T24119 16757-16762 VBG denotes using
T24120 16763-16771 JJ denotes anti-PKC
T24121 16772-16780 NN denotes antibody
T24122 16781-16783 CC denotes or
T24123 16784-16791 NN denotes control
T24124 16792-16795 NNP denotes IgG
T24125 16796-16804 NN denotes antibody
T24126 16804-16805 . denotes .
T24127 16806-16809 CD denotes One
T24128 16810-16814 NN denotes half
T24129 16815-16817 IN denotes of
T24130 16818-16821 DT denotes the
T24131 16822-16829 NNS denotes samples
T24132 16830-16833 VBD denotes was
T24133 16834-16838 VBN denotes used
T24134 16839-16841 TO denotes to
T24135 16842-16849 VB denotes analyze
T24136 16850-16853 NNP denotes PKC
T24137 16854-16860 NN denotes kinase
T24138 16861-16869 NN denotes activity
T24139 16870-16875 VBG denotes using
T24140 16876-16877 DT denotes a
T24141 16878-16889 NN denotes fluorescein
T24142 16890-16896 VBD denotes tagged
T24143 16897-16904 NN denotes peptide
T24144 16905-16908 CC denotes and
T24145 16909-16919 VBN denotes visualized
T24146 16920-16922 IN denotes by
T24147 16923-16930 JJ denotes agarose
T24148 16931-16934 NN denotes gel
T24149 16935-16950 NN denotes electrophoresis
T24150 16951-16952 -LRB- denotes (
T24151 16952-16955 JJ denotes top
T24152 16956-16961 NN denotes panel
T24153 16961-16962 -RRB- denotes )
T24154 16962-16963 , denotes ,
T24155 16964-16969 IN denotes while
T24156 16970-16973 DT denotes the
T24157 16974-16979 JJ denotes other
T24158 16980-16984 NN denotes half
T24159 16985-16988 VBD denotes was
T24160 16989-16998 VBN denotes subjected
T24161 16999-17001 TO denotes to
T24162 17002-17009 JJ denotes Western
T24163 17010-17014 NN denotes blot
T24164 17015-17023 NN denotes analysis
T24165 17024-17026 TO denotes to
T24166 17027-17034 VB denotes confirm
T24167 17035-17040 JJ denotes equal
T24168 17041-17048 NNS denotes amounts
T24169 17049-17051 IN denotes of
T24170 17052-17056 NNP denotes PKCα
T24171 17057-17061 VBD denotes were
T24172 17062-17080 VBN denotes immunoprecipitated
T24173 17081-17085 IN denotes from
T24174 17086-17090 DT denotes each
T24175 17091-17097 NN denotes sample
T24176 17098-17099 -LRB- denotes (
T24177 17099-17105 JJ denotes middle
T24178 17106-17111 NN denotes panel
T24179 17111-17112 -RRB- denotes )
T24180 17112-17113 . denotes .
T24181 17114-17117 DT denotes The
T24182 17118-17124 NN denotes kinase
T24183 17125-17130 NN denotes assay
T24184 17131-17134 VBD denotes was
T24185 17135-17146 VBN denotes quantitated
T24186 17147-17152 VBG denotes using
T24187 17153-17161 NNP denotes Quantity
T24188 17162-17165 CD denotes One
T24189 17166-17174 NN denotes software
T24190 17175-17176 -LRB- denotes (
T24191 17176-17183 NNP denotes Bio-Rad
T24192 17183-17184 -RRB- denotes )
T24193 17185-17186 -LRB- denotes (
T24194 17186-17192 JJ denotes bottom
T24195 17193-17198 NN denotes panel
T24196 17198-17199 -RRB- denotes )
T24197 17199-17200 . denotes .
T24198 17201-17205 NNP denotes Data
T24199 17206-17216 VBZ denotes represents
T24200 17217-17220 DT denotes the
T24201 17221-17228 JJ denotes average
T24202 17229-17233 VBP denotes fold
T24203 17234-17242 NN denotes increase
T24204 17243-17245 IN denotes of
T24205 17246-17250 NN denotes PKCα
T24206 17251-17259 NN denotes activity
T24207 17260-17262 IN denotes in
T24208 17263-17277 JJ denotes non-stimulated
T24209 17278-17285 NNS denotes samples
T24210 17286-17294 VBN denotes compared
T24211 17295-17297 TO denotes to
T24212 17298-17311 JJ denotes M-CSF-treated
T24213 17312-17315 NNP denotes MDM
T24214 17316-17317 NN denotes ±
T24215 17318-17323 NNP denotes S.E.M
T24216 17324-17327 IN denotes for
T24217 17328-17333 CD denotes three
T24218 17334-17345 JJ denotes independent
T24219 17346-17357 NNS denotes experiments
T24220 17357-17358 . denotes .
T24221 17359-17361 NNP denotes NS
T24222 17361-17362 : denotes :
T24223 17363-17377 JJ denotes non-stimulated
T24224 17377-17378 . denotes .
T24225 17379-17380 SYM denotes *
T24226 17381-17384 DT denotes The
T24227 17385-17393 NNS denotes p-values
T24228 17394-17396 IN denotes of
T24229 17397-17402 NNP denotes M-CSF
T24230 17403-17413 VBD denotes stimulated
T24231 17414-17422 VBN denotes compared
T24232 17423-17425 TO denotes to
T24233 17426-17440 JJ denotes non-stimulated
T24234 17441-17445 VBD denotes were
T24235 17446-17447 CD denotes ≤
T24236 17447-17451 CD denotes 0.05
T24237 17451-17452 . denotes .
T7492 17454-17469 JJ denotes M-CSF-Dependent
T7493 17470-17480 NNP denotes Activation
T7494 17481-17483 IN denotes of
T7495 17484-17487 NNP denotes PKC
T7496 17488-17492 VBZ denotes Does
T7497 17493-17496 RB denotes Not
T7498 17497-17505 VB denotes Regulate
T7499 17506-17509 DT denotes the
T7500 17510-17519 JJ denotes Classical
T7501 17520-17525 NN denotes NF-κB
T7502 17526-17529 CD denotes p65
T7503 17530-17540 NNP denotes Activation
T7504 17541-17548 NNP denotes Pathway
T7505 17549-17558 NNP denotes Canonical
T7506 17559-17569 NN denotes activation
T7507 17570-17572 IN denotes of
T7508 17573-17578 JJ denotes NF-κB
T7509 17579-17585 VBZ denotes occurs
T7510 17586-17589 IN denotes via
T7511 17590-17605 NN denotes phosphorylation
T7512 17606-17609 CC denotes and
T7513 17610-17621 NN denotes degradation
T7514 17622-17624 IN denotes of
T7515 17625-17629 NNP denotes IκBα
T7516 17630-17637 VBG denotes leading
T7517 17638-17640 TO denotes to
T7518 17641-17644 DT denotes the
T7519 17645-17652 NN denotes release
T7520 17653-17656 CC denotes and
T7521 17657-17664 JJ denotes nuclear
T7522 17665-17678 NN denotes translocation
T7523 17679-17681 IN denotes of
T7524 17682-17685 DT denotes the
T7525 17686-17691 NNP denotes NF-κB
T7526 17692-17699 CD denotes p50/p65
T7527 17700-17711 NN denotes heterodimer
T7528 17712-17714 TO denotes to
T7529 17715-17728 VB denotes transactivate
T7530 17729-17735 NN denotes target
T7531 17736-17741 NNS denotes genes
T7532 17742-17743 NNP denotes [
T7533 17743-17745 CD denotes 37
T7534 17745-17746 NNP denotes ]
T7535 17746-17747 , denotes ,
T7536 17748-17749 NNP denotes [
T7537 17749-17751 CD denotes 39
T7538 17751-17752 NNP denotes ]
T7539 17752-17753 . denotes .
T7540 17754-17759 IN denotes Since
T7541 17760-17772 JJ denotes conventional
T7542 17773-17776 NNP denotes PKC
T7543 17777-17785 NN denotes activity
T7544 17786-17789 VBD denotes was
T7545 17790-17799 JJ denotes important
T7546 17800-17802 IN denotes in
T7547 17803-17813 VBG denotes regulating
T7548 17814-17827 JJ denotes M-CSF-induced
T7549 17828-17833 NN denotes NF-κB
T7550 17834-17844 NN denotes activation
T7551 17844-17845 , denotes ,
T7552 17846-17848 PRP denotes we
T7553 17849-17853 JJ denotes next
T7554 17854-17866 VBD denotes investigated
T7555 17867-17874 IN denotes whether
T7556 17875-17879 NNP denotes IκBα
T7557 17880-17891 NN denotes degradation
T7558 17892-17895 VBD denotes was
T7559 17896-17905 VBN denotes regulated
T7560 17906-17908 IN denotes by
T7561 17909-17912 NNP denotes PKC
T7562 17912-17913 . denotes .
T7563 17914-17919 NNS denotes Cells
T7564 17920-17924 VBD denotes were
T7565 17925-17932 VBN denotes treated
T7566 17933-17937 IN denotes with
T7567 17938-17951 NN denotes cyclohexamide
T7568 17952-17953 -LRB- denotes (
T7569 17953-17956 NNP denotes CHX
T7570 17956-17957 -RRB- denotes )
T7571 17958-17960 TO denotes to
T7572 17961-17968 VB denotes inhibit
T7573 17969-17976 NN denotes protein
T7574 17977-17986 NN denotes synthesis
T7575 17987-17989 IN denotes of
T7576 17990-17994 NNP denotes IκBα
T7577 17994-17995 , denotes ,
T7578 17996-17999 CC denotes and
T7579 18000-18003 PRP$ denotes its
T7580 18004-18015 NN denotes degradation
T7581 18016-18019 VBD denotes was
T7582 18020-18028 VBN denotes followed
T7583 18028-18029 . denotes .
T7584 18030-18032 IN denotes As
T7585 18033-18038 VBN denotes shown
T7586 18039-18041 IN denotes in
T7587 18042-18048 NN denotes Figure
T7588 18049-18051 CD denotes 5A
T7589 18051-18052 , denotes ,
T7590 18053-18056 NNP denotes PKC
T7591 18057-18067 NN denotes inhibition
T7592 18068-18072 IN denotes with
T7593 18073-18083 NN denotes Ro-31-8220
T7594 18084-18087 VBD denotes did
T7595 18088-18091 RB denotes not
T7596 18092-18097 VB denotes alter
T7597 18098-18111 JJ denotes M-CSF-induced
T7598 18112-18116 NNP denotes IκBα
T7599 18117-18128 NN denotes degradation
T7600 18128-18129 , denotes ,
T7601 18130-18140 VBG denotes suggesting
T7602 18141-18145 IN denotes that
T7603 18146-18159 JJ denotes M-CSF-induced
T7604 18160-18163 NNP denotes PKC
T7605 18164-18172 NN denotes activity
T7606 18173-18182 VBD denotes augmented
T7607 18183-18188 JJ denotes NF-κB
T7608 18189-18204 JJ denotes transcriptional
T7609 18205-18213 NN denotes activity
T7610 18214-18216 IN denotes by
T7611 18217-18219 DT denotes an
T7612 18220-18231 JJ denotes alternative
T7613 18232-18239 NN denotes pathway
T7614 18239-18240 , denotes ,
T7615 18241-18245 IN denotes like
T7616 18246-18264 JJ denotes post-translational
T7617 18265-18277 NN denotes modification
T7618 18278-18280 IN denotes of
T7619 18281-18286 NNP denotes NF-κB
T7620 18287-18290 NNS denotes p65
T7621 18290-18291 . denotes .
T24510 18336-18349 JJ denotes M-CSF-induced
T24511 18350-18353 NN denotes PKC
T24512 18354-18362 NN denotes activity
T24513 18363-18367 VBZ denotes does
T24514 18368-18371 RB denotes not
T24515 18372-18380 VB denotes regulate
T24516 18381-18385 NNP denotes IκBα
T24517 18386-18397 NN denotes degradation
T24518 18398-18401 CC denotes but
T24519 18402-18411 VBZ denotes regulates
T24520 18412-18415 DT denotes the
T24521 18416-18431 NN denotes phosphorylation
T24522 18432-18434 IN denotes of
T24523 18435-18440 NNP denotes NF-κB
T24524 18441-18444 NNS denotes p65
T24525 18445-18447 IN denotes at
T24526 18448-18454 NNP denotes Ser276
T24527 18454-18455 . denotes .
T24528 18456-18457 -LRB- denotes (
T24529 18457-18458 NNP denotes A
T24530 18458-18459 -RRB- denotes )
T24531 18460-18464 NNS denotes MDMs
T24532 18465-18469 VBD denotes were
T24533 18470-18480 VBN denotes pretreated
T24534 18481-18485 IN denotes with
T24535 18486-18499 NN denotes cycloheximide
T24536 18500-18501 -LRB- denotes (
T24537 18501-18504 NNP denotes CHX
T24538 18504-18505 -RRB- denotes )
T24539 18506-18508 IN denotes in
T24540 18509-18512 DT denotes the
T24541 18513-18520 NN denotes absence
T24542 18521-18523 CC denotes or
T24543 18524-18532 NN denotes presence
T24544 18533-18535 IN denotes of
T24545 18536-18546 NN denotes Ro-31-8220
T24546 18547-18550 IN denotes for
T24547 18551-18553 CD denotes 30
T24548 18554-18561 NNS denotes minutes
T24549 18562-18567 RB denotes prior
T24550 18568-18570 TO denotes to
T24551 18571-18576 NNP denotes M-CSF
T24552 18577-18588 NN denotes stimulation
T24553 18589-18592 IN denotes for
T24554 18593-18596 DT denotes the
T24555 18597-18606 JJ denotes indicated
T24556 18607-18612 NNS denotes times
T24557 18612-18613 . denotes .
T24558 18614-18619 JJ denotes Whole
T24559 18620-18624 NN denotes cell
T24560 18625-18632 NNS denotes lysates
T24561 18633-18637 VBD denotes were
T24562 18638-18647 VBN denotes subjected
T24563 18648-18650 TO denotes to
T24564 18651-18658 JJ denotes Western
T24565 18659-18667 VBG denotes blotting
T24566 18668-18672 IN denotes with
T24567 18673-18682 JJ denotes anti-IκBα
T24568 18683-18691 NN denotes antibody
T24569 18691-18692 . denotes .
T24570 18693-18697 NNS denotes Data
T24571 18698-18701 VBP denotes are
T24572 18702-18716 NN denotes representative
T24573 18717-18719 IN denotes of
T24574 18720-18725 CD denotes three
T24575 18726-18737 JJ denotes independent
T24576 18738-18749 NNS denotes experiments
T24577 18749-18750 . denotes .
T24578 18751-18752 -LRB- denotes (
T24579 18752-18753 NNP denotes B
T24580 18753-18754 -RRB- denotes )
T24581 18755-18759 NNS denotes MDMs
T24582 18760-18764 VBD denotes were
T24583 18765-18775 VBN denotes pretreated
T24584 18776-18780 IN denotes with
T24585 18781-18787 DT denotes either
T24586 18788-18795 NN denotes vehicle
T24587 18796-18798 CC denotes or
T24588 18799-18809 NN denotes Ro-31-8220
T24589 18810-18813 IN denotes for
T24590 18814-18816 CD denotes 30
T24591 18817-18824 NNS denotes minutes
T24592 18825-18831 IN denotes before
T24593 18832-18835 DT denotes the
T24594 18836-18844 NN denotes addition
T24595 18845-18847 IN denotes of
T24596 18848-18853 NNP denotes M-CSF
T24597 18854-18857 IN denotes for
T24598 18858-18860 CD denotes 10
T24599 18861-18868 NNS denotes minutes
T24600 18868-18869 . denotes .
T24601 18870-18875 JJ denotes Whole
T24602 18876-18880 NN denotes cell
T24603 18881-18888 NNS denotes lysates
T24604 18889-18893 VBD denotes were
T24605 18894-18902 VBN denotes resolved
T24606 18903-18905 IN denotes by
T24607 18906-18914 NNP denotes SDS-PAGE
T24608 18915-18918 CC denotes and
T24609 18919-18933 NNP denotes phospho-Ser276
T24610 18934-18936 CC denotes or
T24611 18937-18951 NNP denotes phospho-Ser536
T24612 18952-18954 IN denotes of
T24613 18955-18960 NNP denotes NF-κB
T24614 18961-18964 CD denotes p65
T24615 18965-18968 VBD denotes was
T24616 18969-18977 VBN denotes detected
T24617 18978-18983 VBG denotes using
T24618 18984-19000 JJ denotes phospho-specific
T24619 19001-19011 NNS denotes antibodies
T24620 19012-19014 TO denotes to
T24621 19015-19021 DT denotes either
T24622 19022-19029 NN denotes residue
T24623 19030-19032 IN denotes of
T24624 19033-19038 NNP denotes NF-κB
T24625 19039-19042 NNS denotes p65
T24626 19042-19043 . denotes .
T24627 19044-19045 -LRB- denotes (
T24628 19045-19046 $ denotes C
T24629 19046-19047 -RRB- denotes )
T24630 19048-19053 JJ denotes Whole
T24631 19054-19058 NN denotes cell
T24632 19059-19066 NNS denotes lysates
T24633 19067-19069 IN denotes of
T24634 19070-19073 NNP denotes RAW
T24635 19074-19079 CD denotes 264.7
T24636 19080-19085 NNS denotes cells
T24637 19086-19093 VBN denotes treated
T24638 19094-19098 IN denotes with
T24639 19099-19106 NN denotes vehicle
T24640 19107-19114 NN denotes control
T24641 19115-19117 CC denotes or
T24642 19118-19128 NN denotes Ro-31-8220
T24643 19129-19131 IN denotes in
T24644 19132-19135 DT denotes the
T24645 19136-19143 NN denotes absence
T24646 19144-19146 CC denotes or
T24647 19147-19155 NN denotes presence
T24648 19156-19158 IN denotes of
T24649 19159-19164 NNP denotes M-CSF
T24650 19165-19169 VBD denotes were
T24651 19170-19179 VBN denotes subjected
T24652 19180-19182 TO denotes to
T24653 19183-19190 JJ denotes Western
T24654 19191-19195 NN denotes blot
T24655 19196-19204 NN denotes analysis
T24656 19205-19209 IN denotes with
T24657 19210-19224 NN denotes phospho-Ser276
T24658 19225-19227 CC denotes or
T24659 19228-19242 NN denotes phospho-Ser536
T24660 19243-19248 NNP denotes NF-κB
T24661 19249-19252 CD denotes p65
T24662 19253-19263 NNS denotes antibodies
T24663 19263-19264 . denotes .
T24664 19265-19266 -LRB- denotes (
T24665 19266-19267 NNP denotes D
T24666 19267-19268 -RRB- denotes )
T24667 19269-19278 NNP denotes Cytosolic
T24668 19279-19282 CC denotes and
T24669 19283-19290 JJ denotes nuclear
T24670 19291-19300 NNS denotes fractions
T24671 19301-19303 IN denotes of
T24672 19304-19307 NNP denotes RAW
T24673 19308-19313 CD denotes 264.7
T24674 19314-19318 VBD denotes were
T24675 19319-19327 VBN denotes obtained
T24676 19328-19332 IN denotes from
T24677 19333-19336 DT denotes the
T24678 19337-19344 VBN denotes treated
T24679 19345-19350 NNS denotes cells
T24680 19351-19354 CC denotes and
T24681 19355-19368 VBN denotes immunoblotted
T24682 19369-19372 IN denotes for
T24683 19373-19386 NN denotes phospho-NF-κB
T24684 19386-19387 . denotes .
T24685 19388-19391 DT denotes The
T24686 19392-19398 NN denotes purity
T24687 19399-19401 IN denotes of
T24688 19402-19405 DT denotes the
T24689 19406-19415 JJ denotes cytosolic
T24690 19416-19419 CC denotes and
T24691 19420-19427 JJ denotes nuclear
T24692 19428-19437 NNS denotes fractions
T24693 19438-19441 VBD denotes was
T24694 19442-19451 VBN denotes confirmed
T24695 19452-19454 IN denotes by
T24696 19455-19469 VBG denotes immunoblotting
T24697 19470-19474 IN denotes with
T24698 19475-19480 NNP denotes GAPDH
T24699 19481-19484 CC denotes and
T24700 19485-19490 NNP denotes Lamin
T24701 19491-19492 NNP denotes B
T24702 19492-19493 , denotes ,
T24703 19494-19506 RB denotes respectively
T24704 19506-19507 . denotes .
T24705 19508-19513 VBN denotes Shown
T24706 19514-19517 VBP denotes are
T24707 19518-19532 JJ denotes representative
T24708 19533-19538 NNS denotes blots
T24709 19539-19543 IN denotes from
T24710 19544-19549 CD denotes three
T24711 19550-19561 JJ denotes independent
T24712 19562-19573 NNS denotes experiments
T24713 19573-19574 . denotes .
T8089 19576-19581 NNP denotes M-CSF
T8090 19582-19589 NNP denotes Induces
T8091 19590-19605 NNP denotes Phosphorylation
T8092 19606-19608 IN denotes of
T8093 19609-19614 NNP denotes NF-κB
T8094 19615-19621 NNP denotes Ser276
T8095 19622-19624 IN denotes in
T8096 19625-19626 DT denotes a
T8097 19627-19640 JJ denotes PKC-dependent
T8098 19641-19648 NN denotes Fashion
T8099 19649-19654 IN denotes Since
T8100 19655-19660 NNP denotes M-CSF
T8101 19661-19664 VBD denotes did
T8102 19665-19668 RB denotes not
T8103 19669-19677 VB denotes regulate
T8104 19678-19683 JJ denotes NF-κB
T8105 19684-19694 NN denotes activation
T8106 19695-19697 IN denotes by
T8107 19698-19709 VBG denotes influencing
T8108 19710-19714 NNP denotes IκBα
T8109 19714-19715 , denotes ,
T8110 19716-19718 PRP denotes we
T8111 19719-19723 JJ denotes next
T8112 19724-19730 VBN denotes sought
T8113 19731-19733 TO denotes to
T8114 19734-19743 VB denotes determine
T8115 19744-19746 IN denotes if
T8116 19747-19752 NNP denotes M-CSF
T8117 19753-19761 VBD denotes affected
T8118 19762-19767 NNP denotes NF-κB
T8119 19768-19771 CD denotes p65
T8120 19772-19774 IN denotes by
T8121 19775-19793 JJ denotes post-translational
T8122 19794-19804 NNS denotes mechanisms
T8123 19804-19805 . denotes .
T8124 19806-19810 RB denotes Thus
T8125 19810-19811 , denotes ,
T8126 19812-19814 PRP denotes we
T8127 19815-19823 VBD denotes examined
T8128 19824-19827 DT denotes the
T8129 19828-19843 NN denotes phosphorylation
T8130 19844-19846 IN denotes of
T8131 19847-19852 NNP denotes NF-κB
T8132 19853-19856 CD denotes p65
T8133 19857-19861 IN denotes with
T8134 19862-19870 JJ denotes specific
T8135 19871-19883 NN denotes phospho-NFκB
T8136 19884-19887 NNS denotes p65
T8137 19888-19889 -LRB- denotes (
T8138 19889-19895 CD denotes Ser276
T8139 19896-19899 CC denotes and
T8140 19900-19906 NNP denotes Ser536
T8141 19906-19907 -RRB- denotes )
T8142 19908-19918 NNS denotes antibodies
T8143 19918-19919 . denotes .
T8144 19920-19925 NNP denotes M-CSF
T8145 19926-19933 VBD denotes induced
T8146 19934-19937 DT denotes the
T8147 19938-19953 NN denotes phosphorylation
T8148 19954-19956 IN denotes of
T8149 19957-19963 NNP denotes Ser276
T8150 19964-19967 CC denotes but
T8151 19968-19971 RB denotes not
T8152 19972-19978 NNP denotes Ser536
T8153 19979-19981 IN denotes of
T8154 19982-19987 NNP denotes NF-κB
T8155 19988-19991 CD denotes p65
T8156 19992-19994 IN denotes in
T8157 19995-19999 NNS denotes MDMs
T8158 19999-20000 . denotes .
T8159 20001-20009 VBN denotes Compared
T8160 20010-20012 TO denotes to
T8161 20013-20020 NN denotes vehicle
T8162 20020-20021 , denotes ,
T8163 20022-20025 DT denotes the
T8164 20026-20033 JJ denotes general
T8165 20034-20037 NNP denotes PKC
T8166 20038-20047 NN denotes inhibitor
T8167 20048-20058 NN denotes Ro-31-8220
T8168 20059-20066 VBN denotes reduced
T8169 20067-20073 NNP denotes Ser276
T8170 20074-20089 NN denotes phosphorylation
T8171 20089-20090 , denotes ,
T8172 20091-20094 CC denotes but
T8173 20095-20098 RB denotes not
T8174 20099-20105 NNP denotes Ser536
T8175 20105-20106 , denotes ,
T8176 20107-20122 NN denotes phosphorylation
T8177 20123-20125 IN denotes in
T8178 20126-20142 JJ denotes M-CSF-stimulated
T8179 20143-20148 NNS denotes cells
T8180 20149-20150 -LRB- denotes (
T8181 20150-20156 NN denotes Figure
T8182 20157-20159 NN denotes 5B
T8183 20159-20160 -RRB- denotes )
T8184 20160-20161 . denotes .
T8185 20162-20173 RB denotes Furthermore
T8186 20173-20174 , denotes ,
T8187 20175-20191 JJ denotes M-CSF-stimulated
T8188 20192-20197 NNP denotes NF-κB
T8189 20198-20201 CD denotes p65
T8190 20202-20217 NN denotes phosphorylation
T8191 20218-20220 IN denotes at
T8192 20221-20228 NN denotes residue
T8193 20229-20235 NNP denotes Ser276
T8194 20236-20238 IN denotes in
T8195 20239-20242 NNP denotes RAW
T8196 20243-20248 CD denotes 264.7
T8197 20249-20254 NNS denotes cells
T8198 20255-20258 VBD denotes was
T8199 20259-20263 RB denotes also
T8200 20264-20267 NNP denotes PKC
T8201 20268-20277 JJ denotes dependent
T8202 20278-20279 -LRB- denotes (
T8203 20279-20285 NN denotes Figure
T8204 20286-20288 NN denotes 5C
T8205 20288-20289 -RRB- denotes )
T8206 20289-20290 . denotes .
T8207 20291-20296 DT denotes These
T8208 20297-20304 NNS denotes studies
T8209 20305-20314 VBD denotes suggested
T8210 20315-20319 IN denotes that
T8211 20320-20323 NNP denotes PKC
T8212 20323-20324 -LRB- denotes (
T8213 20324-20325 PRP denotes s
T8214 20325-20326 -RRB- denotes )
T8215 20327-20336 VBN denotes regulated
T8216 20337-20343 NNP denotes Ser276
T8217 20344-20359 NN denotes phosphorylation
T8218 20360-20363 CC denotes but
T8219 20364-20367 RB denotes not
T8220 20368-20374 NNP denotes Ser536
T8221 20375-20377 IN denotes in
T8222 20378-20382 DT denotes both
T8223 20383-20388 JJ denotes human
T8224 20389-20393 NNS denotes MDMs
T8225 20394-20397 CC denotes and
T8226 20398-20403 NN denotes mouse
T8227 20404-20415 NNS denotes macrophages
T8228 20416-20421 IN denotes after
T8229 20422-20427 NNP denotes M-CSF
T8230 20428-20439 NN denotes stimulation
T8231 20439-20440 . denotes .
T8232 20441-20443 PRP denotes We
T8233 20444-20448 JJ denotes next
T8234 20449-20458 VBN denotes performed
T8235 20459-20467 JJ denotes cellular
T8236 20468-20481 NN denotes fractionation
T8237 20482-20484 TO denotes to
T8238 20485-20493 VB denotes identify
T8239 20494-20497 DT denotes the
T8240 20498-20506 JJ denotes cellular
T8241 20507-20515 NN denotes location
T8242 20516-20518 IN denotes of
T8243 20519-20533 VBN denotes phosphorylated
T8244 20534-20539 NNP denotes NF-κB
T8245 20540-20543 NNS denotes p65
T8246 20544-20546 IN denotes in
T8247 20547-20550 NNP denotes Raw
T8248 20551-20556 CD denotes 264.7
T8249 20557-20562 NNS denotes cells
T8250 20562-20563 . denotes .
T8251 20564-20582 JJ denotes Non-phosphorylated
T8252 20583-20588 NNP denotes NF-κB
T8253 20589-20592 NNS denotes p65
T8254 20593-20596 VBD denotes was
T8255 20597-20604 VBN denotes located
T8256 20605-20607 IN denotes in
T8257 20608-20612 DT denotes both
T8258 20613-20622 JJ denotes cytosolic
T8259 20623-20626 CC denotes and
T8260 20627-20634 JJ denotes nuclear
T8261 20635-20644 NNS denotes fractions
T8262 20644-20645 , denotes ,
T8263 20646-20649 CC denotes but
T8264 20650-20664 VBD denotes phosphorylated
T8265 20665-20671 NNP denotes Ser276
T8266 20672-20675 CC denotes and
T8267 20676-20682 NNP denotes Ser536
T8268 20683-20688 NNP denotes NF-κB
T8269 20689-20692 CD denotes p65
T8270 20693-20696 VBD denotes was
T8271 20697-20706 RB denotes primarily
T8272 20707-20714 VBN denotes located
T8273 20715-20717 IN denotes in
T8274 20718-20725 JJ denotes nuclear
T8275 20726-20734 NN denotes fraction
T8276 20735-20740 IN denotes after
T8277 20741-20746 NNP denotes M-CSF
T8278 20747-20758 NN denotes stimulation
T8279 20759-20760 -LRB- denotes (
T8280 20760-20766 NN denotes Figure
T8281 20767-20769 CD denotes 5D
T8282 20769-20770 -RRB- denotes )
T8283 20770-20771 . denotes .
T8284 20772-20779 RB denotes Notably
T8285 20779-20780 , denotes ,
T8286 20781-20793 JJ denotes constitutive
T8287 20794-20809 NN denotes phosphorylation
T8288 20810-20812 IN denotes of
T8289 20813-20819 NNP denotes Ser536
T8290 20820-20825 NNP denotes NF-κB
T8291 20826-20829 CD denotes p65
T8292 20830-20833 VBD denotes was
T8293 20834-20839 VBN denotes found
T8294 20840-20842 IN denotes in
T8295 20843-20848 DT denotes these
T8296 20849-20854 NNS denotes cells
T8297 20854-20855 . denotes .
T8298 20856-20867 RB denotes Importantly
T8299 20867-20868 , denotes ,
T8300 20869-20879 JJ denotes Ro-31-8220
T8301 20880-20887 JJ denotes reduced
T8302 20888-20901 JJ denotes M-CSF-induced
T8303 20902-20908 NNP denotes Ser276
T8304 20909-20924 NN denotes phosphorylation
T8305 20925-20927 IN denotes of
T8306 20928-20933 NNP denotes NF-κB
T8307 20934-20937 CD denotes p65
T8308 20938-20940 IN denotes in
T8309 20941-20945 CC denotes both
T8310 20946-20949 DT denotes the
T8311 20950-20959 JJ denotes cytosolic
T8312 20960-20963 CC denotes and
T8313 20964-20971 JJ denotes nuclear
T8314 20972-20981 NNS denotes fractions
T8315 20981-20982 , denotes ,
T8316 20983-20988 IN denotes while
T8317 20989-21002 NNP denotes M-CSF-induced
T8318 21003-21008 NNP denotes NF-κB
T8319 21009-21012 CD denotes p65
T8320 21013-21019 NNP denotes Ser536
T8321 21020-21035 NN denotes phosphorylation
T8322 21036-21039 VBD denotes was
T8323 21040-21047 JJ denotes present
T8324 21048-21050 IN denotes in
T8325 21051-21054 DT denotes the
T8326 21055-21062 NN denotes nucleus
T8327 21063-21073 RB denotes regardless
T8328 21074-21076 IN denotes of
T8329 21077-21080 NNP denotes PKC
T8330 21081-21091 NN denotes inhibition
T8331 21091-21092 . denotes .
T8332 21093-21098 DT denotes These
T8333 21099-21111 NNS denotes observations
T8334 21112-21120 VBP denotes indicate
T8335 21121-21125 IN denotes that
T8336 21126-21139 JJ denotes M-CSF-induced
T8337 21140-21146 NNP denotes Ser276
T8338 21147-21150 CC denotes and
T8339 21151-21157 NNP denotes Ser536
T8340 21158-21161 VBP denotes are
T8341 21162-21171 VBN denotes regulated
T8342 21172-21183 RB denotes differently
T8343 21184-21186 IN denotes by
T8344 21187-21199 JJ denotes conventional
T8345 21200-21203 NNP denotes PKC
T8346 21204-21214 NN denotes activation
T8347 21215-21217 IN denotes in
T8348 21218-21229 NN denotes mononuclear
T8349 21230-21240 NNS denotes phagocytes
T8350 21240-21241 . denotes .
T8351 21242-21245 DT denotes The
T8352 21246-21252 NN denotes purity
T8353 21253-21255 IN denotes of
T8354 21256-21259 DT denotes the
T8355 21260-21267 NN denotes cytosol
T8356 21268-21271 CC denotes and
T8357 21272-21279 JJ denotes nuclear
T8358 21280-21284 NN denotes cell
T8359 21285-21294 NNS denotes fractions
T8360 21295-21298 VBD denotes was
T8361 21299-21308 VBN denotes confirmed
T8362 21309-21311 IN denotes by
T8363 21312-21326 VBG denotes immunoblotting
T8364 21327-21331 IN denotes with
T8365 21332-21337 NNP denotes GAPDH
T8366 21338-21341 CC denotes and
T8367 21342-21347 NNP denotes Lamin
T8368 21348-21349 NNP denotes B
T8369 21349-21350 , denotes ,
T8370 21351-21363 RB denotes respectively
T8371 21364-21365 -LRB- denotes (
T8372 21365-21371 NN denotes Figure
T8373 21372-21374 CD denotes 5D
T8374 21374-21375 -RRB- denotes )
T8375 21375-21376 . denotes .
T9427 21378-21393 JJ denotes M-CSF-dependent
T9428 21394-21397 NNP denotes PKC
T9429 21398-21407 VBZ denotes Regulates
T9430 21408-21422 JJ denotes NF-κB-targeted
T9431 21423-21428 NNS denotes Genes
T9432 21429-21434 JJ denotes NF-κB
T9433 21435-21442 VBZ denotes induces
T9434 21443-21444 DT denotes a
T9435 21445-21451 NN denotes number
T9436 21452-21454 IN denotes of
T9437 21455-21465 JJ denotes downstream
T9438 21466-21471 NNS denotes genes
T9439 21471-21472 , denotes ,
T9440 21473-21482 VBG denotes including
T9441 21483-21486 DT denotes the
T9442 21487-21490 NNP denotes IκB
T9443 21491-21497 NN denotes family
T9444 21497-21498 . denotes .
T9445 21499-21504 IN denotes Among
T9446 21505-21508 DT denotes the
T9447 21509-21512 NNP denotes IκB
T9448 21513-21522 NNS denotes molecules
T9449 21522-21523 , denotes ,
T9450 21524-21528 NNP denotes IκBα
T9451 21529-21531 VBZ denotes is
T9452 21532-21538 RB denotes highly
T9453 21539-21546 VBN denotes induced
T9454 21547-21549 IN denotes by
T9455 21550-21555 JJ denotes NF-κB
T9456 21556-21566 NN denotes activation
T9457 21567-21568 NNP denotes [
T9458 21568-21570 CD denotes 40
T9459 21570-21571 NNP denotes ]
T9460 21571-21572 . denotes .
T9461 21573-21579 VBG denotes Having
T9462 21580-21585 VBN denotes shown
T9463 21586-21590 IN denotes that
T9464 21591-21594 NNP denotes PKC
T9465 21595-21604 VBD denotes regulated
T9466 21605-21610 JJ denotes NF-κB
T9467 21611-21619 NN denotes activity
T9468 21620-21622 IN denotes in
T9469 21623-21639 JJ denotes M-CSF-stimulated
T9470 21640-21644 NNS denotes MDMs
T9471 21644-21645 , denotes ,
T9472 21646-21648 PRP denotes we
T9473 21649-21653 JJ denotes next
T9474 21654-21664 VBN denotes determined
T9475 21665-21672 IN denotes whether
T9476 21673-21683 NN denotes inhibition
T9477 21684-21686 IN denotes of
T9478 21687-21690 NNP denotes PKC
T9479 21691-21699 NN denotes activity
T9480 21700-21709 VBD denotes decreased
T9481 21710-21720 NN denotes expression
T9482 21721-21723 IN denotes of
T9483 21724-21739 JJ denotes NF-κB-regulated
T9484 21740-21745 NNS denotes genes
T9485 21745-21746 . denotes .
T9486 21747-21749 PRP denotes We
T9487 21750-21757 VBD denotes treated
T9488 21758-21762 DT denotes both
T9489 21763-21767 NNS denotes MDMs
T9490 21768-21771 CC denotes and
T9491 21772-21775 NNP denotes RAW
T9492 21776-21781 CD denotes 264.7
T9493 21782-21787 NNS denotes cells
T9494 21788-21792 IN denotes with
T9495 21793-21796 DT denotes the
T9496 21797-21800 NNP denotes PKC
T9497 21801-21810 NN denotes inhibitor
T9498 21811-21821 NN denotes Ro-31-8220
T9499 21822-21825 IN denotes for
T9500 21826-21828 CD denotes 30
T9501 21829-21836 NNS denotes minutes
T9502 21837-21840 CC denotes and
T9503 21841-21845 RB denotes then
T9504 21846-21856 VBN denotes stimulated
T9505 21857-21861 IN denotes with
T9506 21862-21867 NNP denotes M-CSF
T9507 21867-21868 . denotes .
T9508 21869-21873 NNP denotes IκBα
T9509 21874-21878 NN denotes gene
T9510 21879-21882 VBD denotes was
T9511 21883-21891 VBN denotes measured
T9512 21892-21894 IN denotes by
T9513 21895-21902 NNP denotes qRT-PCR
T9514 21902-21903 . denotes .
T9515 21904-21906 IN denotes As
T9516 21907-21912 VBN denotes shown
T9517 21913-21915 IN denotes in
T9518 21916-21923 NNS denotes Figures
T9519 21924-21926 NNP denotes 6A
T9520 21927-21930 CC denotes and
T9521 21931-21933 NNP denotes 6B
T9522 21933-21934 , denotes ,
T9523 21935-21940 NNP denotes M-CSF
T9524 21941-21949 VBD denotes enhanced
T9525 21950-21954 NNP denotes IκBα
T9526 21955-21959 NN denotes gene
T9527 21960-21970 NN denotes expression
T9528 21971-21974 CC denotes and
T9529 21975-21978 NNP denotes PKC
T9530 21979-21989 NN denotes inhibition
T9531 21990-21992 IN denotes by
T9532 21993-22003 JJ denotes Ro-31-8220
T9533 22004-22013 VBN denotes decreased
T9534 22014-22018 NNP denotes IκBα
T9535 22019-22023 NN denotes gene
T9536 22024-22034 NN denotes expression
T9537 22035-22037 IN denotes in
T9538 22038-22042 DT denotes both
T9539 22043-22047 NNS denotes MDMs
T9540 22048-22051 CC denotes and
T9541 22052-22055 NNP denotes RAW
T9542 22056-22061 CD denotes 264.7
T9543 22062-22067 NNS denotes cells
T9544 22068-22069 -LRB- denotes (
T9545 22069-22070 FW denotes p
T9546 22070-22071 FW denotes <
T9547 22071-22075 CD denotes 0.01
T9548 22075-22076 -RRB- denotes )
T9549 22076-22077 , denotes ,
T9550 22078-22091 VBG denotes demonstrating
T9551 22092-22096 IN denotes that
T9552 22097-22100 NNP denotes PKC
T9553 22101-22109 VBD denotes affected
T9554 22110-22125 JJ denotes NF-κB-regulated
T9555 22126-22130 NN denotes gene
T9556 22131-22141 NN denotes expression
T9557 22142-22144 IN denotes in
T9558 22145-22156 NNS denotes macrophages
T9559 22156-22157 . denotes .
T25121 22202-22212 NNP denotes Inhibition
T25122 22213-22215 IN denotes of
T25123 22216-22229 NNP denotes M-CSF-induced
T25124 22230-22233 NNP denotes PKC
T25125 22234-22241 VBZ denotes reduces
T25126 22242-22257 JJ denotes NF-κB-regulated
T25127 22258-22263 NNS denotes genes
T25128 22264-22266 IN denotes in
T25129 22267-22271 DT denotes both
T25130 22272-22276 NNS denotes MDMs
T25131 22277-22280 CC denotes and
T25132 22281-22284 NNP denotes RAW
T25133 22285-22290 CD denotes 264.7
T25134 22291-22296 NNS denotes cells
T25135 22296-22297 . denotes .
T25136 22298-22302 NNS denotes MDMs
T25137 22303-22304 -LRB- denotes (
T25138 22304-22305 DT denotes A
T25139 22306-22309 CC denotes and
T25140 22310-22311 NNP denotes C
T25141 22311-22312 -RRB- denotes )
T25142 22313-22316 CC denotes and
T25143 22317-22320 NNP denotes RAW
T25144 22321-22326 CD denotes 264.7
T25145 22327-22328 -LRB- denotes (
T25146 22328-22329 NN denotes B
T25147 22329-22330 -RRB- denotes )
T25148 22331-22336 NNS denotes cells
T25149 22337-22341 VBD denotes were
T25150 22342-22352 VBN denotes pretreated
T25151 22353-22357 IN denotes with
T25152 22358-22368 NN denotes Ro-31-8220
T25153 22369-22371 CC denotes or
T25154 22372-22379 JJ denotes solvent
T25155 22380-22387 NN denotes control
T25156 22388-22392 NNP denotes DMSO
T25157 22393-22396 IN denotes for
T25158 22397-22399 CD denotes 30
T25159 22400-22407 NNS denotes minutes
T25160 22408-22413 RB denotes prior
T25161 22414-22416 TO denotes to
T25162 22417-22422 NNP denotes M-CSF
T25163 22423-22434 NN denotes stimulation
T25164 22435-22438 IN denotes for
T25165 22439-22442 DT denotes the
T25166 22443-22452 JJ denotes indicated
T25167 22453-22458 NNS denotes times
T25168 22458-22459 . denotes .
T25169 22460-22465 JJ denotes Total
T25170 22466-22469 NNP denotes RNA
T25171 22470-22473 VBD denotes was
T25172 22474-22482 VBN denotes isolated
T25173 22483-22486 CC denotes and
T25174 22487-22496 VBN denotes converted
T25175 22497-22499 TO denotes to
T25176 22500-22504 NNP denotes cDNA
T25177 22504-22505 . denotes .
T25178 22506-22515 JJ denotes Real-time
T25179 22516-22518 NNP denotes RT
T25180 22519-22522 NNP denotes PCR
T25181 22523-22526 VBD denotes was
T25182 22527-22536 VBN denotes performed
T25183 22537-22542 VBG denotes using
T25184 22543-22550 NNS denotes primers
T25185 22551-22554 IN denotes for
T25186 22555-22559 NNP denotes IκBα
T25187 22559-22560 , denotes ,
T25188 22561-22567 NNP denotes BCL-xl
T25189 22568-22570 CC denotes or
T25190 22571-22576 NNP denotes GAPDH
T25191 22576-22577 . denotes .
T25192 22578-22582 NNS denotes Data
T25193 22583-22586 VBP denotes are
T25194 22587-22596 VBN denotes expressed
T25195 22597-22599 IN denotes as
T25196 22600-22608 JJ denotes relative
T25197 22609-22613 VBP denotes fold
T25198 22614-22622 NN denotes increase
T25199 22623-22625 IN denotes of
T25200 22626-22630 NNP denotes IκBα
T25201 22631-22633 CC denotes or
T25202 22634-22640 NNP denotes BCL-xl
T25203 22641-22645 NN denotes gene
T25204 22646-22656 NN denotes expression
T25205 22657-22661 IN denotes upon
T25206 22662-22671 NN denotes treatment
T25207 22672-22676 IN denotes over
T25208 22677-22691 JJ denotes non-stimulated
T25209 22692-22697 NNS denotes cells
T25210 22697-22698 . denotes .
T25211 22699-22703 NNP denotes Data
T25212 22704-22713 VBP denotes represent
T25213 22714-22717 DT denotes the
T25214 22718-22722 JJ denotes mean
T25215 22723-22724 NN denotes ±
T25216 22725-22730 NNP denotes S.E.M
T25217 22731-22734 IN denotes for
T25218 22735-22740 CD denotes three
T25219 22741-22752 JJ denotes independent
T25220 22753-22764 NNS denotes experiments
T25221 22764-22765 . denotes .
T9560 22768-22770 TO denotes To
T9561 22771-22778 RBR denotes further
T9562 22779-22785 VB denotes define
T9563 22786-22789 DT denotes the
T9564 22790-22794 NN denotes role
T9565 22795-22797 IN denotes of
T9566 22798-22801 NNP denotes PKC
T9567 22802-22804 IN denotes in
T9568 22805-22814 VBG denotes mediating
T9569 22815-22820 JJ denotes human
T9570 22821-22824 NNP denotes MDM
T9571 22825-22833 NN denotes survival
T9572 22834-22836 IN denotes in
T9573 22837-22845 NN denotes response
T9574 22846-22848 TO denotes to
T9575 22849-22854 NNP denotes M-CSF
T9576 22854-22855 , denotes ,
T9577 22856-22858 PRP denotes we
T9578 22859-22867 VBD denotes examined
T9579 22868-22871 DT denotes the
T9580 22872-22882 NN denotes expression
T9581 22883-22885 IN denotes of
T9582 22886-22889 DT denotes the
T9583 22890-22904 JJ denotes anti-apoptotic
T9584 22905-22909 NN denotes gene
T9585 22910-22916 NNP denotes BCL-xL
T9586 22916-22917 , denotes ,
T9587 22918-22923 WDT denotes which
T9588 22924-22926 VBZ denotes is
T9589 22927-22931 RB denotes also
T9590 22932-22941 VBN denotes regulated
T9591 22942-22944 IN denotes by
T9592 22945-22950 NNP denotes NF-κB
T9593 22950-22951 . denotes .
T9594 22952-22954 IN denotes As
T9595 22955-22960 VBN denotes shown
T9596 22961-22963 IN denotes in
T9597 22964-22970 NNP denotes Figure
T9598 22971-22973 NNP denotes 6C
T9599 22973-22974 , denotes ,
T9600 22975-22985 NNP denotes Ro-31-8220
T9601 22986-22993 VBD denotes reduced
T9602 22994-23010 JJ denotes M-CSF-stimulated
T9603 23011-23017 JJ denotes BCL-xL
T9604 23018-23028 NN denotes expression
T9605 23029-23037 VBN denotes compared
T9606 23038-23040 TO denotes to
T9607 23041-23046 NNS denotes cells
T9608 23047-23054 VBN denotes treated
T9609 23055-23059 IN denotes with
T9610 23060-23065 NNP denotes M-CSF
T9611 23066-23069 CC denotes and
T9612 23070-23073 DT denotes the
T9613 23074-23081 NN denotes vehicle
T9614 23082-23089 NN denotes control
T9615 23090-23094 NNP denotes DMSO
T9616 23095-23096 -LRB- denotes (
T9617 23096-23097 JJ denotes p
T9618 23097-23098 NN denotes <
T9619 23098-23102 CD denotes 0.05
T9620 23102-23103 -RRB- denotes )
T9621 23103-23104 . denotes .
T10239 23106-23120 NN denotes Identification
T10240 23121-23123 IN denotes of
T10241 23124-23128 NNP denotes PKCα
T10242 23129-23131 IN denotes as
T10243 23132-23135 DT denotes the
T10244 23136-23144 NNP denotes Upstream
T10245 23145-23154 NNP denotes Activator
T10246 23155-23157 IN denotes of
T10247 23158-23163 NNP denotes NF-κB
T10248 23164-23166 IN denotes in
T10249 23167-23174 NNP denotes Myeloid
T10250 23175-23180 NNP denotes Cells
T10251 23181-23185 RB denotes Even
T10252 23186-23192 IN denotes though
T10253 23193-23196 DT denotes the
T10254 23197-23200 NNP denotes PKC
T10255 23201-23207 NN denotes family
T10256 23208-23216 VBZ denotes consists
T10257 23217-23219 IN denotes of
T10258 23220-23222 CD denotes 10
T10259 23223-23230 NNS denotes members
T10260 23230-23231 , denotes ,
T10261 23232-23239 VBG denotes finding
T10262 23240-23244 IN denotes that
T10263 23245-23249 NNP denotes PKCα
T10264 23249-23250 NNP denotes /
T10265 23250-23251 VBD denotes β
T10266 23252-23262 NNS denotes inhibitors
T10267 23263-23266 CC denotes and
T10268 23267-23280 JJ denotes intracellular
T10269 23281-23288 NN denotes calcium
T10270 23289-23299 NNS denotes inhibitors
T10271 23300-23307 VBD denotes reduced
T10272 23308-23321 JJ denotes M-CSF-induced
T10273 23322-23327 NN denotes NF-κB
T10274 23328-23336 NN denotes activity
T10275 23336-23337 , denotes ,
T10276 23338-23347 VBD denotes suggested
T10277 23348-23352 NNP denotes PKCα
T10278 23353-23356 VBD denotes was
T10279 23357-23365 VBN denotes involved
T10280 23366-23368 IN denotes in
T10281 23369-23374 JJ denotes NF-κB
T10282 23375-23385 NN denotes activation
T10283 23386-23391 IN denotes after
T10284 23392-23397 NNP denotes M-CSF
T10285 23398-23407 NN denotes treatment
T10286 23407-23408 . denotes .
T10287 23409-23411 TO denotes To
T10288 23412-23419 VB denotes confirm
T10289 23420-23423 DT denotes the
T10290 23424-23428 NN denotes role
T10291 23429-23431 IN denotes of
T10292 23432-23436 NNP denotes PKCα
T10293 23437-23439 IN denotes in
T10294 23440-23445 NNP denotes NF-κB
T10295 23446-23456 NN denotes activation
T10296 23457-23459 IN denotes in
T10297 23460-23471 NNS denotes macrophages
T10298 23471-23472 , denotes ,
T10299 23473-23483 NNS denotes constructs
T10300 23484-23487 IN denotes for
T10301 23488-23494 DT denotes either
T10302 23495-23503 NN denotes wildtype
T10303 23504-23505 -LRB- denotes (
T10304 23505-23507 NNP denotes WT
T10305 23507-23508 -RRB- denotes )
T10306 23508-23509 : denotes -
T10307 23509-23513 NN denotes PKCα
T10308 23514-23516 CC denotes or
T10309 23517-23533 NN denotes kinase-deficient
T10310 23534-23535 -LRB- denotes (
T10311 23535-23537 NNP denotes KD
T10312 23537-23538 -RRB- denotes )
T10313 23538-23539 : denotes -
T10314 23539-23543 NN denotes PKCα
T10315 23544-23547 VBD denotes was
T10316 23548-23562 VBN denotes co-transfected
T10317 23563-23567 IN denotes with
T10318 23568-23571 DT denotes the
T10319 23572-23583 JJ denotes pNF-κB-SEAP
T10320 23584-23592 NN denotes reporter
T10321 23593-23597 NN denotes gene
T10322 23598-23601 CC denotes and
T10323 23602-23606 NNP denotes SEAP
T10324 23607-23616 NN denotes secretion
T10325 23617-23620 VBD denotes was
T10326 23621-23629 VBN denotes measured
T10327 23629-23630 . denotes .
T10328 23631-23633 IN denotes As
T10329 23634-23639 VBN denotes shown
T10330 23640-23642 IN denotes in
T10331 23643-23649 NN denotes Figure
T10332 23650-23652 NN denotes 7A
T10333 23652-23653 , denotes ,
T10334 23654-23658 NNP denotes MDMs
T10335 23659-23673 VBD denotes co-transfected
T10336 23674-23678 IN denotes with
T10337 23679-23690 NNP denotes pNF-κB-SEAP
T10338 23691-23694 CC denotes and
T10339 23695-23702 NNP denotes WT-PKCα
T10340 23703-23706 VBD denotes had
T10341 23707-23708 DT denotes a
T10342 23709-23717 JJ denotes 1.8-fold
T10343 23718-23726 NN denotes increase
T10344 23727-23729 IN denotes in
T10345 23730-23735 JJ denotes NF-κB
T10346 23736-23751 JJ denotes transcriptional
T10347 23752-23760 NN denotes activity
T10348 23761-23766 IN denotes after
T10349 23767-23772 NNP denotes M-CSF
T10350 23773-23783 NN denotes activation
T10351 23784-23792 VBN denotes compared
T10352 23793-23797 IN denotes with
T10353 23798-23800 NNP denotes NS
T10354 23801-23806 NNS denotes cells
T10355 23807-23808 -LRB- denotes (
T10356 23808-23809 FW denotes p
T10357 23810-23811 SYM denotes =
T10358 23812-23816 CD denotes 0.05
T10359 23816-23817 -RRB- denotes )
T10360 23817-23818 , denotes ,
T10361 23819-23826 JJ denotes similar
T10362 23827-23829 TO denotes to
T10363 23830-23843 JJ denotes M-CSF-treated
T10364 23844-23849 NNS denotes cells
T10365 23850-23860 VBG denotes expressing
T10366 23861-23865 RB denotes only
T10367 23866-23877 JJ denotes pNF-κB-SEAP
T10368 23877-23878 . denotes .
T10369 23879-23891 VBG denotes Transfecting
T10370 23892-23897 JJ denotes human
T10371 23898-23909 NNS denotes macrophages
T10372 23910-23914 IN denotes with
T10373 23915-23918 DT denotes the
T10374 23919-23926 NNP denotes KD-PKCα
T10375 23927-23936 VBP denotes construct
T10376 23937-23950 RB denotes significantly
T10377 23951-23958 VBN denotes reduced
T10378 23959-23972 JJ denotes M-CSF-induced
T10379 23973-23978 NN denotes NF-κB
T10380 23979-23987 NN denotes activity
T10381 23988-23996 VBN denotes compared
T10382 23997-23999 TO denotes to
T10383 24000-24007 JJ denotes WT-PKCα
T10384 24008-24019 VBN denotes transfected
T10385 24020-24025 NNS denotes cells
T10386 24026-24027 -LRB- denotes (
T10387 24027-24028 FW denotes p
T10388 24029-24030 SYM denotes =
T10389 24031-24036 CD denotes 0.016
T10390 24036-24037 -RRB- denotes )
T10391 24037-24038 . denotes .
T10392 24039-24048 RB denotes Similarly
T10393 24048-24049 , denotes ,
T10394 24050-24053 NNP denotes RAW
T10395 24054-24059 CD denotes 264.7
T10396 24060-24065 NNS denotes cells
T10397 24066-24077 VBN denotes transfected
T10398 24078-24082 IN denotes with
T10399 24083-24090 NNP denotes WT-PKCα
T10400 24091-24094 VBD denotes had
T10401 24095-24103 CD denotes 2.5-fold
T10402 24104-24108 JJR denotes more
T10403 24109-24114 JJ denotes NF-κB
T10404 24115-24130 NN denotes transcriptional
T10405 24131-24139 NN denotes activity
T10406 24140-24145 IN denotes after
T10407 24146-24151 NNP denotes M-CSF
T10408 24152-24162 NN denotes activation
T10409 24163-24171 VBN denotes compared
T10410 24172-24174 TO denotes to
T10411 24175-24187 JJ denotes unstimulated
T10412 24188-24191 NNP denotes RAW
T10413 24192-24197 CD denotes 264.7
T10414 24198-24203 NNS denotes cells
T10415 24204-24205 -LRB- denotes (
T10416 24205-24207 NNP denotes NS
T10417 24207-24208 -RRB- denotes )
T10418 24209-24220 VBN denotes transfected
T10419 24221-24225 IN denotes with
T10420 24226-24233 NNP denotes WT-PKCα
T10421 24234-24235 -LRB- denotes (
T10422 24235-24241 NNP denotes Figure
T10423 24242-24244 NN denotes 7B
T10424 24244-24245 -RRB- denotes )
T10425 24245-24246 . denotes .
T10426 24247-24257 NN denotes Expression
T10427 24258-24260 IN denotes of
T10428 24261-24264 DT denotes the
T10429 24265-24272 NNP denotes KD-PKCα
T10430 24273-24282 VBP denotes construct
T10431 24283-24287 IN denotes into
T10432 24288-24291 NNP denotes RAW
T10433 24292-24297 CD denotes 264.7
T10434 24298-24303 NNS denotes cells
T10435 24304-24311 VBN denotes reduced
T10436 24312-24325 JJ denotes M-CSF-induced
T10437 24326-24331 NN denotes NF-κB
T10438 24332-24340 NN denotes activity
T10439 24341-24343 TO denotes to
T10440 24344-24352 CD denotes 1.5-fold
T10441 24353-24354 -LRB- denotes (
T10442 24354-24355 NN denotes p
T10443 24356-24357 SYM denotes =
T10444 24358-24363 CD denotes 0.045
T10445 24363-24364 -RRB- denotes )
T10446 24365-24373 VBN denotes compared
T10447 24374-24376 TO denotes to
T10448 24377-24382 NNS denotes cells
T10449 24383-24394 VBN denotes transfected
T10450 24395-24399 IN denotes with
T10451 24400-24402 NNP denotes WT
T10452 24403-24407 NNP denotes PKCα
T10453 24407-24408 . denotes .
T25475 24453-24457 NNP denotes PKCα
T25476 24458-24467 VBZ denotes regulates
T25477 24468-24483 NN denotes phosphorylation
T25478 24484-24486 IN denotes of
T25479 24487-24492 NNP denotes NF-κB
T25480 24493-24496 NNS denotes p65
T25481 24497-24499 IN denotes at
T25482 24500-24506 NNP denotes Ser276
T25483 24506-24507 . denotes .
T25484 24508-24509 -LRB- denotes (
T25485 24509-24510 NNP denotes A
T25486 24510-24511 -RRB- denotes )
T25487 24512-24515 NNP denotes MDM
T25488 24516-24518 CC denotes or
T25489 24519-24520 -LRB- denotes (
T25490 24520-24521 NNP denotes B
T25491 24521-24522 -RRB- denotes )
T25492 24523-24526 NNP denotes RAW
T25493 24527-24532 CD denotes 264.7
T25494 24533-24538 NNS denotes cells
T25495 24539-24543 VBD denotes were
T25496 24544-24555 RB denotes transiently
T25497 24556-24567 VBN denotes transfected
T25498 24568-24572 IN denotes with
T25499 24573-24584 JJ denotes pNF-κB-SEAP
T25500 24585-24590 IN denotes along
T25501 24591-24595 IN denotes with
T25502 24596-24602 DT denotes either
T25503 24603-24610 NNP denotes WT-PKCα
T25504 24611-24613 CC denotes or
T25505 24614-24617 DT denotes the
T25506 24618-24634 JJ denotes kinase-deficient
T25507 24635-24636 -LRB- denotes (
T25508 24636-24638 NNP denotes KD
T25509 24638-24639 -RRB- denotes )
T25510 24639-24640 : denotes -
T25511 24640-24644 NNP denotes PKCα
T25512 24645-24654 VB denotes construct
T25513 24655-24657 IN denotes at
T25514 24658-24659 DT denotes a
T25515 24660-24661 CD denotes 1
T25516 24661-24662 NN denotes ∶
T25517 24662-24663 CD denotes 5
T25518 24664-24669 NN denotes ratio
T25519 24669-24670 . denotes .
T25520 24671-24676 NNS denotes Cells
T25521 24677-24681 VBD denotes were
T25522 24682-24687 JJ denotes serum
T25523 24688-24695 VBN denotes starved
T25524 24696-24699 CC denotes and
T25525 24700-24710 VBN denotes stimulated
T25526 24711-24715 IN denotes with
T25527 24716-24721 NNP denotes M-CSF
T25528 24722-24725 CC denotes and
T25529 24726-24730 RB denotes then
T25530 24731-24735 NNP denotes SEAP
T25531 24736-24745 NN denotes secretion
T25532 24746-24748 IN denotes in
T25533 24749-24752 DT denotes the
T25534 24753-24759 NN denotes medium
T25535 24760-24763 VBD denotes was
T25536 24764-24772 VBN denotes measured
T25537 24772-24773 . denotes .
T25538 24774-24778 NNP denotes Data
T25539 24779-24781 VBZ denotes is
T25540 24782-24786 IN denotes from
T25541 24787-24789 IN denotes of
T25542 24790-24795 CD denotes three
T25543 24796-24807 JJ denotes independent
T25544 24808-24819 NNS denotes experiments
T25545 24819-24820 . denotes .
T25546 24821-24824 DT denotes The
T25547 24825-24832 NN denotes p-value
T25548 24833-24835 IN denotes of
T25549 24836-24841 NNS denotes cells
T25550 24842-24853 VBN denotes transfected
T25551 24854-24858 IN denotes with
T25552 24859-24861 NNP denotes KD
T25553 24862-24870 VBN denotes compared
T25554 24871-24873 TO denotes to
T25555 24874-24879 DT denotes those
T25556 24880-24891 VBN denotes transfected
T25557 24892-24896 IN denotes with
T25558 24897-24899 NNP denotes WT
T25559 24900-24903 VBD denotes was
T25560 24904-24908 CD denotes 0.05
T25561 24908-24909 . denotes .
T25562 24910-24911 -LRB- denotes (
T25563 24911-24912 $ denotes C
T25564 24912-24913 -RRB- denotes )
T25565 24914-24918 NNS denotes MDMs
T25566 24919-24923 VBD denotes were
T25567 24924-24931 VBN denotes removed
T25568 24932-24936 IN denotes from
T25569 24937-24940 DT denotes the
T25570 24941-24946 NN denotes plate
T25571 24947-24952 VBG denotes using
T25572 24953-24961 NN denotes accutase
T25573 24962-24965 CC denotes and
T25574 24966-24975 NN denotes apoptosis
T25575 24976-24978 IN denotes of
T25576 24979-24983 NNS denotes MDMs
T25577 24984-24987 VBD denotes was
T25578 24988-24996 VBN denotes measured
T25579 24997-24999 IN denotes by
T25580 25000-25004 NN denotes flow
T25581 25005-25014 NN denotes cytometry
T25582 25015-25020 VBG denotes using
T25583 25021-25028 NNP denotes Annexin
T25584 25029-25035 NNP denotes V-FITC
T25585 25036-25039 CC denotes and
T25586 25040-25049 NN denotes propidium
T25587 25050-25056 NN denotes iodine
T25588 25057-25058 -LRB- denotes (
T25589 25058-25060 NNP denotes PI
T25590 25060-25061 -RRB- denotes )
T25591 25061-25062 . denotes .
T25592 25063-25064 -LRB- denotes (
T25593 25064-25065 NNP denotes D
T25594 25065-25066 -RRB- denotes )
T25595 25067-25072 NNP denotes Whole
T25596 25073-25077 NN denotes cell
T25597 25078-25085 VBZ denotes lysates
T25598 25086-25090 IN denotes from
T25599 25091-25094 DT denotes the
T25600 25095-25106 VBN denotes transfected
T25601 25107-25110 NNP denotes RAW
T25602 25111-25116 CD denotes 264.7
T25603 25117-25122 NNS denotes cells
T25604 25123-25127 VBD denotes were
T25605 25128-25137 VBN denotes subjected
T25606 25138-25140 TO denotes to
T25607 25141-25148 JJ denotes Western
T25608 25149-25153 NN denotes blot
T25609 25154-25162 NN denotes analysis
T25610 25163-25167 IN denotes with
T25611 25168-25182 NN denotes phospho-Ser276
T25612 25183-25185 CC denotes or
T25613 25186-25200 NN denotes phospho-Ser536
T25614 25201-25206 NNP denotes NF-κB
T25615 25207-25210 CD denotes p65
T25616 25211-25221 NNS denotes antibodies
T25617 25221-25222 . denotes .
T25618 25223-25228 NNS denotes Blots
T25619 25229-25233 VBD denotes were
T25620 25234-25247 VBN denotes immunoblotted
T25621 25248-25252 IN denotes with
T25622 25253-25257 NNP denotes PKCα
T25623 25258-25260 TO denotes to
T25624 25261-25270 VB denotes determine
T25625 25271-25276 JJ denotes equal
T25626 25277-25284 NN denotes protein
T25627 25285-25295 NN denotes expression
T25628 25296-25299 IN denotes for
T25629 25300-25303 DT denotes the
T25630 25304-25308 NNP denotes PKCα
T25631 25309-25319 NNS denotes constructs
T25632 25319-25320 . denotes .
T25633 25321-25328 JJ denotes β-actin
T25634 25329-25335 VBD denotes served
T25635 25336-25338 IN denotes as
T25636 25339-25340 DT denotes a
T25637 25341-25348 VBG denotes loading
T25638 25349-25356 NN denotes control
T25639 25356-25357 . denotes .
T25640 25358-25363 VBN denotes Shown
T25641 25364-25366 VBZ denotes is
T25642 25367-25368 DT denotes a
T25643 25369-25383 JJ denotes representative
T25644 25384-25388 NN denotes blot
T25645 25389-25393 IN denotes from
T25646 25394-25399 CD denotes three
T25647 25400-25411 JJ denotes independent
T25648 25412-25423 NNS denotes experiments
T25649 25423-25424 . denotes .
T25650 25425-25426 -LRB- denotes (
T25651 25426-25427 NNP denotes E
T25652 25427-25428 -RRB- denotes )
T25653 25429-25432 NNP denotes MDM
T25654 25433-25435 CC denotes or
T25655 25436-25437 -LRB- denotes (
T25656 25437-25438 NN denotes F
T25657 25438-25439 -RRB- denotes )
T25658 25440-25443 NNP denotes RAW
T25659 25444-25449 CD denotes 264.7
T25660 25450-25455 NNS denotes cells
T25661 25456-25460 VBD denotes were
T25662 25461-25472 RB denotes transiently
T25663 25473-25484 VBN denotes transfected
T25664 25485-25489 IN denotes with
T25665 25490-25491 DT denotes a
T25666 25492-25503 JJ denotes pNF-κB-SEAP
T25667 25504-25509 IN denotes along
T25668 25510-25514 IN denotes with
T25669 25515-25521 DT denotes either
T25670 25522-25525 CD denotes 100
T25671 25526-25528 NNP denotes nM
T25672 25529-25533 NNP denotes PKCα
T25673 25534-25539 NNP denotes siRNA
T25674 25540-25542 CC denotes or
T25675 25543-25550 VB denotes control
T25676 25551-25556 NNP denotes siRNA
T25677 25557-25560 IN denotes for
T25678 25561-25566 CD denotes 20-24
T25679 25567-25572 NNS denotes hours
T25680 25572-25573 . denotes .
T25681 25574-25579 NNS denotes Cells
T25682 25580-25584 VBD denotes were
T25683 25585-25590 JJ denotes serum
T25684 25591-25598 VBN denotes starved
T25685 25599-25602 IN denotes for
T25686 25603-25606 CD denotes 2-4
T25687 25607-25612 NNS denotes hours
T25688 25613-25616 CC denotes and
T25689 25617-25627 VBN denotes stimulated
T25690 25628-25632 IN denotes with
T25691 25633-25636 CD denotes 100
T25692 25637-25642 JJ denotes ng/ml
T25693 25643-25648 NN denotes M-CSF
T25694 25649-25652 IN denotes for
T25695 25653-25654 CD denotes 6
T25696 25655-25660 NNS denotes hours
T25697 25661-25664 IN denotes for
T25698 25665-25668 NNP denotes MDM
T25699 25669-25671 CC denotes or
T25700 25672-25675 NNP denotes RAW
T25701 25676-25681 CD denotes 264.7
T25702 25682-25685 IN denotes for
T25703 25686-25687 CD denotes 2
T25704 25688-25693 NNS denotes hours
T25705 25694-25697 CC denotes and
T25706 25698-25702 RB denotes then
T25707 25703-25707 NNP denotes SEAP
T25708 25708-25717 NN denotes secretion
T25709 25718-25720 IN denotes in
T25710 25721-25724 DT denotes the
T25711 25725-25731 NN denotes medium
T25712 25732-25735 VBD denotes was
T25713 25736-25744 VBN denotes measured
T25714 25744-25745 . denotes .
T25715 25746-25751 VBN denotes Shown
T25716 25752-25754 VBZ denotes is
T25717 25755-25759 NNS denotes data
T25718 25760-25762 IN denotes of
T25719 25763-25768 CD denotes three
T25720 25769-25780 JJ denotes independent
T25721 25781-25792 NNS denotes experiments
T25722 25792-25793 . denotes .
T25723 25794-25795 -LRB- denotes (
T25724 25795-25796 NNP denotes G
T25725 25796-25797 -RRB- denotes )
T25726 25798-25802 NNS denotes MDMs
T25727 25803-25807 VBD denotes were
T25728 25808-25815 VBN denotes removed
T25729 25816-25820 IN denotes from
T25730 25821-25824 DT denotes the
T25731 25825-25830 NN denotes plate
T25732 25831-25836 VBG denotes using
T25733 25837-25845 NN denotes accutase
T25734 25846-25849 CC denotes and
T25735 25850-25859 NN denotes apoptosis
T25736 25860-25862 IN denotes of
T25737 25863-25867 NNS denotes MDMs
T25738 25868-25871 VBD denotes was
T25739 25872-25880 VBN denotes measured
T25740 25881-25883 IN denotes by
T25741 25884-25891 NNP denotes Annexin
T25742 25892-25898 NNP denotes V-FITC
T25743 25899-25902 CC denotes and
T25744 25903-25912 NN denotes propidium
T25745 25913-25919 NN denotes iodine
T25746 25920-25921 -LRB- denotes (
T25747 25921-25923 NNP denotes PI
T25748 25923-25924 -RRB- denotes )
T25749 25925-25933 VBG denotes staining
T25750 25934-25937 CC denotes and
T25751 25938-25946 VBN denotes analyzed
T25752 25947-25949 IN denotes by
T25753 25950-25954 NN denotes flow
T25754 25955-25964 NN denotes cytometry
T25755 25964-25965 . denotes .
T25756 25966-25967 -LRB- denotes (
T25757 25967-25968 NNP denotes H
T25758 25968-25969 -RRB- denotes )
T25759 25970-25975 NNP denotes Whole
T25760 25976-25980 NN denotes cell
T25761 25981-25988 VBZ denotes lysates
T25762 25989-25993 IN denotes from
T25763 25994-25997 DT denotes the
T25764 25998-26009 VBN denotes transfected
T25765 26010-26013 NNP denotes MDM
T25766 26014-26017 CC denotes and
T25767 26018-26021 NNP denotes RAW
T25768 26022-26027 CD denotes 264.7
T25769 26028-26033 NNS denotes cells
T25770 26034-26038 VBD denotes were
T25771 26039-26048 VBN denotes subjected
T25772 26049-26051 TO denotes to
T25773 26052-26059 JJ denotes Western
T25774 26060-26064 NN denotes blot
T25775 26065-26073 NN denotes analysis
T25776 26074-26078 IN denotes with
T25777 26079-26083 NNP denotes PKCα
T25778 26084-26092 NN denotes antibody
T25779 26092-26093 . denotes .
T25780 26094-26101 JJ denotes β-actin
T25781 26102-26108 VBD denotes served
T25782 26109-26111 IN denotes as
T25783 26112-26113 DT denotes a
T25784 26114-26121 VBG denotes loading
T25785 26122-26129 NN denotes control
T25786 26129-26130 . denotes .
T25787 26131-26136 VBN denotes Shown
T25788 26137-26139 VBZ denotes is
T25789 26140-26141 DT denotes a
T25790 26142-26156 JJ denotes representative
T25791 26157-26161 NN denotes blot
T25792 26162-26166 IN denotes from
T25793 26167-26169 IN denotes at
T25794 26170-26175 JJS denotes least
T25795 26176-26181 CD denotes three
T25796 26182-26193 JJ denotes independent
T25797 26194-26205 NNS denotes experiments
T25798 26205-26206 . denotes .
T10454 26209-26213 NNP denotes Cell
T10455 26214-26222 NN denotes survival
T10456 26223-26226 VBD denotes was
T10457 26227-26231 RB denotes also
T10458 26232-26240 VBN denotes examined
T10459 26241-26243 IN denotes in
T10460 26244-26248 NNS denotes MDMs
T10461 26249-26259 VBG denotes expressing
T10462 26260-26266 CC denotes either
T10463 26267-26274 NNP denotes WT-PKCα
T10464 26275-26277 CC denotes or
T10465 26278-26285 NNP denotes KD-PKCα
T10466 26286-26296 NNS denotes constructs
T10467 26297-26299 IN denotes by
T10468 26300-26307 NNP denotes Annexin
T10469 26308-26314 NNP denotes V-FITC
T10470 26315-26318 CC denotes and
T10471 26319-26321 NNP denotes PI
T10472 26322-26330 VBG denotes staining
T10473 26330-26331 . denotes .
T10474 26332-26334 IN denotes As
T10475 26335-26343 VBN denotes expected
T10476 26343-26344 , denotes ,
T10477 26345-26350 NNP denotes M-CSF
T10478 26351-26360 VBD denotes increased
T10479 26361-26364 NNP denotes MDM
T10480 26365-26373 NN denotes survival
T10481 26374-26376 IN denotes as
T10482 26377-26385 VBN denotes measured
T10483 26386-26388 IN denotes by
T10484 26389-26392 DT denotes the
T10485 26393-26400 NN denotes percent
T10486 26401-26403 IN denotes of
T10487 26404-26411 NNP denotes Annexin
T10488 26412-26416 NNP denotes V/PI
T10489 26417-26425 JJ denotes negative
T10490 26426-26431 NNS denotes cells
T10491 26431-26432 . denotes .
T10492 26433-26442 RB denotes Similarly
T10493 26442-26443 , denotes ,
T10494 26444-26454 NN denotes expression
T10495 26455-26457 IN denotes of
T10496 26458-26465 JJ denotes WT-PKCα
T10497 26466-26475 JJ denotes protected
T10498 26476-26481 NNS denotes cells
T10499 26482-26486 IN denotes from
T10500 26487-26496 NN denotes apoptosis
T10501 26496-26497 . denotes .
T10502 26498-26500 IN denotes In
T10503 26501-26509 NN denotes contrast
T10504 26509-26510 , denotes ,
T10505 26511-26521 NN denotes expression
T10506 26522-26524 IN denotes of
T10507 26525-26532 NNP denotes KD-PKCα
T10508 26533-26542 VBD denotes decreased
T10509 26543-26556 JJ denotes M-CSF-induced
T10510 26557-26561 NN denotes cell
T10511 26562-26570 NN denotes survival
T10512 26571-26572 -LRB- denotes (
T10513 26572-26573 FW denotes p
T10514 26573-26574 FW denotes <
T10515 26574-26578 CD denotes 0.01
T10516 26578-26579 -RRB- denotes )
T10517 26580-26581 -LRB- denotes (
T10518 26581-26587 NN denotes Figure
T10519 26588-26590 NN denotes 7C
T10520 26590-26591 -RRB- denotes )
T10521 26591-26592 . denotes .
T10522 26593-26597 JJ denotes Next
T10523 26597-26598 , denotes ,
T10524 26599-26601 PRP denotes we
T10525 26602-26610 VBD denotes examined
T10526 26611-26614 DT denotes the
T10527 26615-26621 NN denotes effect
T10528 26622-26624 IN denotes of
T10529 26625-26635 VBG denotes expressing
T10530 26636-26639 DT denotes the
T10531 26640-26644 NNP denotes PKCα
T10532 26645-26655 NNS denotes constructs
T10533 26656-26658 IN denotes on
T10534 26659-26664 JJ denotes NF-κB
T10535 26665-26680 NN denotes phosphorylation
T10536 26680-26681 . denotes .
T10537 26682-26684 IN denotes As
T10538 26685-26690 VBN denotes shown
T10539 26691-26693 IN denotes in
T10540 26694-26700 NN denotes Figure
T10541 26701-26703 CD denotes 7D
T10542 26703-26704 , denotes ,
T10543 26705-26715 NN denotes expression
T10544 26716-26718 IN denotes of
T10545 26719-26726 NNP denotes KD-PKCα
T10546 26727-26729 IN denotes in
T10547 26730-26733 NNP denotes RAW
T10548 26734-26739 CD denotes 264.7
T10549 26740-26745 NNS denotes cells
T10550 26746-26749 VBD denotes did
T10551 26750-26753 RB denotes not
T10552 26754-26760 VB denotes affect
T10553 26761-26764 DT denotes the
T10554 26765-26777 JJ denotes constitutive
T10555 26778-26793 NN denotes phosphorylation
T10556 26794-26796 IN denotes at
T10557 26797-26803 NNP denotes Ser536
T10558 26804-26806 IN denotes of
T10559 26807-26812 NNP denotes NF-κB
T10560 26813-26816 CD denotes p65
T10561 26816-26817 , denotes ,
T10562 26818-26821 CC denotes but
T10563 26822-26832 VBD denotes attenuated
T10564 26833-26836 DT denotes the
T10565 26837-26852 NN denotes phosphorylation
T10566 26853-26855 IN denotes at
T10567 26856-26862 NNP denotes Ser276
T10568 26862-26863 . denotes .
T10569 26864-26874 NN denotes Expression
T10570 26875-26877 IN denotes of
T10571 26878-26885 NNP denotes WT-PKCα
T10572 26886-26889 VBD denotes did
T10573 26890-26893 RB denotes not
T10574 26894-26900 NN denotes effect
T10575 26901-26904 DT denotes the
T10576 26905-26920 NN denotes phosphorylation
T10577 26921-26923 IN denotes of
T10578 26924-26930 DT denotes either
T10579 26931-26938 NN denotes residue
T10580 26939-26943 IN denotes with
T10581 26944-26946 CC denotes or
T10582 26947-26954 IN denotes without
T10583 26955-26960 NNP denotes M-CSF
T10584 26961-26972 NN denotes stimulation
T10585 26972-26973 . denotes .
T10586 26974-26979 DT denotes These
T10587 26980-26992 NNS denotes observations
T10588 26993-27004 VBP denotes demonstrate
T10589 27005-27009 IN denotes that
T10590 27010-27014 NNP denotes PKCα
T10591 27015-27017 VBZ denotes is
T10592 27018-27027 JJ denotes important
T10593 27028-27030 IN denotes in
T10594 27031-27046 JJ denotes M-CSF-regulated
T10595 27047-27051 NN denotes cell
T10596 27052-27060 NN denotes survival
T10597 27061-27064 CC denotes and
T10598 27065-27070 JJ denotes NF-κB
T10599 27071-27081 NN denotes activation
T10600 27082-27085 CC denotes and
T10601 27086-27092 JJ denotes likely
T10602 27093-27102 VBN denotes regulated
T10603 27103-27110 IN denotes through
T10604 27111-27126 NN denotes phosphorylation
T10605 27127-27129 IN denotes of
T10606 27130-27136 NNP denotes Ser276
T10607 27137-27139 IN denotes of
T10608 27140-27145 NNP denotes NF-κB
T10609 27146-27149 NNS denotes p65
T10610 27149-27150 . denotes .
T10611 27151-27153 TO denotes To
T10612 27154-27161 RBR denotes further
T10613 27162-27170 VB denotes validate
T10614 27171-27174 DT denotes the
T10615 27175-27181 NN denotes impact
T10616 27182-27186 WDT denotes that
T10617 27187-27191 NNP denotes PKCα
T10618 27192-27198 VBD denotes played
T10619 27199-27201 IN denotes in
T10620 27202-27215 JJ denotes M-CSF-induced
T10621 27216-27221 NN denotes NF-κB
T10622 27222-27237 JJ denotes transcriptional
T10623 27238-27246 NN denotes activity
T10624 27246-27247 , denotes ,
T10625 27248-27250 PRP denotes we
T10626 27251-27255 JJ denotes next
T10627 27256-27264 VBN denotes employed
T10628 27265-27269 NNP denotes PKCα
T10629 27270-27275 NNP denotes siRNA
T10630 27276-27285 NN denotes treatment
T10631 27286-27288 IN denotes of
T10632 27289-27292 NNP denotes MDM
T10633 27293-27295 CC denotes or
T10634 27296-27299 NNP denotes RAW
T10635 27300-27305 NNS denotes cells
T10636 27305-27306 . denotes .
T10637 27307-27308 DT denotes A
T10638 27309-27313 NN denotes pool
T10639 27314-27316 IN denotes of
T10640 27317-27325 JJ denotes specific
T10641 27326-27330 NNP denotes PKCα
T10642 27331-27336 NNP denotes siRNA
T10643 27337-27341 VBD denotes were
T10644 27342-27353 VBN denotes transfected
T10645 27354-27358 IN denotes into
T10646 27359-27362 NNP denotes MDM
T10647 27363-27365 CC denotes or
T10648 27366-27369 NNP denotes Raw
T10649 27370-27375 CD denotes 264.7
T10650 27376-27381 NNS denotes cells
T10651 27382-27384 IN denotes in
T10652 27385-27388 DT denotes the
T10653 27389-27397 NN denotes presence
T10654 27398-27400 CC denotes or
T10655 27401-27408 NN denotes absence
T10656 27409-27411 CC denotes or
T10657 27412-27417 NN denotes M-CSF
T10658 27417-27418 . denotes .
T10659 27419-27427 VBG denotes Reducing
T10660 27428-27434 JJ denotes native
T10661 27435-27439 NNP denotes PKCα
T10662 27440-27450 NN denotes expression
T10663 27451-27460 VBD denotes decreased
T10664 27461-27474 JJ denotes M-CSF-induced
T10665 27475-27480 NN denotes NF-κB
T10666 27481-27496 JJ denotes transcriptional
T10667 27497-27505 NN denotes activity
T10668 27506-27508 IN denotes in
T10669 27509-27513 DT denotes both
T10670 27514-27517 NNP denotes MDM
T10671 27518-27519 -LRB- denotes (
T10672 27519-27525 NNP denotes Figure
T10673 27526-27528 NNP denotes 7E
T10674 27528-27529 -RRB- denotes )
T10675 27530-27531 -LRB- denotes (
T10676 27531-27532 FW denotes p
T10677 27533-27534 SYM denotes =
T10678 27535-27540 CD denotes 0.012
T10679 27540-27541 -RRB- denotes )
T10680 27542-27545 CC denotes and
T10681 27546-27549 NNP denotes Raw
T10682 27550-27555 CD denotes 264.7
T10683 27556-27561 NNS denotes cells
T10684 27562-27563 -LRB- denotes (
T10685 27563-27569 NN denotes Figure
T10686 27570-27572 CD denotes 7F
T10687 27572-27573 -RRB- denotes )
T10688 27574-27575 -LRB- denotes (
T10689 27575-27576 FW denotes p
T10690 27577-27578 SYM denotes =
T10691 27579-27583 CD denotes 0.01
T10692 27583-27584 -RRB- denotes )
T10693 27584-27585 . denotes .
T10694 27586-27588 PRP denotes We
T10695 27589-27593 RB denotes also
T10696 27594-27602 VBD denotes examined
T10697 27603-27607 NN denotes cell
T10698 27608-27616 NN denotes survival
T10699 27617-27619 IN denotes of
T10700 27620-27623 DT denotes the
T10701 27624-27628 NNS denotes MDMs
T10702 27629-27631 IN denotes by
T10703 27632-27639 NNP denotes Annexin
T10704 27640-27646 NNP denotes V-FITC
T10705 27647-27650 CC denotes and
T10706 27651-27653 NNP denotes PI
T10707 27654-27662 VBG denotes staining
T10708 27663-27668 IN denotes after
T10709 27669-27673 NNP denotes PKCα
T10710 27674-27679 NNP denotes siRNA
T10711 27680-27692 NN denotes transfection
T10712 27692-27693 . denotes .
T10713 27694-27696 IN denotes As
T10714 27697-27702 VBN denotes shown
T10715 27703-27705 IN denotes in
T10716 27706-27712 NN denotes Figure
T10717 27713-27715 CD denotes 7G
T10718 27715-27716 , denotes ,
T10719 27717-27730 JJ denotes M-CSF-induced
T10720 27731-27734 NNP denotes MDM
T10721 27735-27743 NN denotes survival
T10722 27744-27747 VBD denotes was
T10723 27748-27755 VBN denotes reduced
T10724 27756-27758 IN denotes in
T10725 27759-27762 DT denotes the
T10726 27763-27768 NNS denotes cells
T10727 27769-27780 VBN denotes transfected
T10728 27781-27785 IN denotes with
T10729 27786-27790 NNP denotes PKCα
T10730 27791-27796 NNP denotes siRNA
T10731 27797-27805 VBN denotes compared
T10732 27806-27810 IN denotes with
T10733 27811-27816 NNS denotes cells
T10734 27817-27828 VBN denotes transfected
T10735 27829-27833 IN denotes with
T10736 27834-27841 NN denotes control
T10737 27842-27847 NNP denotes siRNA
T10738 27848-27849 -LRB- denotes (
T10739 27849-27850 FW denotes p
T10740 27851-27852 SYM denotes =
T10741 27853-27858 CD denotes 0.047
T10742 27858-27859 -RRB- denotes )
T10743 27859-27860 . denotes .
T10744 27861-27863 IN denotes In
T10745 27864-27870 NN denotes Figure
T10746 27871-27873 CD denotes 7H
T10747 27873-27874 , denotes ,
T10748 27875-27877 PRP denotes we
T10749 27878-27887 VBD denotes confirmed
T10750 27888-27892 IN denotes that
T10751 27893-27897 NNP denotes PKCα
T10752 27898-27903 NNP denotes siRNA
T10753 27904-27916 NN denotes transfection
T10754 27917-27926 VBD denotes decreased
T10755 27927-27931 NNP denotes PKCα
T10756 27932-27939 NN denotes protein
T10757 27940-27950 NN denotes expression
T10758 27951-27953 IN denotes in
T10759 27954-27958 DT denotes both
T10760 27959-27962 NNP denotes MDM
T10761 27963-27966 CC denotes and
T10762 27967-27970 NNP denotes Raw
T10763 27971-27976 CD denotes 264.7
T10764 27977-27982 NNS denotes cells
T10765 27982-27983 . denotes .
T10766 27984-27987 PRP$ denotes Our
T10767 27988-27995 NNS denotes results
T10768 27996-28005 VBD denotes indicated
T10769 28006-28010 IN denotes that
T10770 28011-28015 NNP denotes PKCα
T10771 28016-28025 VBD denotes regulated
T10772 28026-28031 NNP denotes NF-κB
T10773 28032-28042 NN denotes activation
T10774 28043-28046 CC denotes and
T10775 28047-28062 JJ denotes M-CSF-regulated
T10776 28063-28067 NN denotes cell
T10777 28068-28076 NN denotes survival
T10778 28076-28077 . denotes .
T12350 28079-28082 NNP denotes PKC
T12351 28083-28085 VBZ denotes is
T12352 28086-28095 JJ denotes Essential
T12353 28096-28098 IN denotes in
T12354 28099-28109 VBG denotes Regulating
T12355 28110-28115 NNP denotes NF-κB
T12356 28116-28124 NNP denotes Activity
T12357 28125-28140 NNP denotes M-CSF-dependent
T12358 28141-28144 NNP denotes PKC
T12359 28145-28153 NN denotes activity
T12360 28154-28165 VBD denotes facilitated
T12361 28166-28171 NNP denotes NF-κB
T12362 28172-28175 CD denotes p65
T12363 28176-28191 NN denotes phosphorylation
T12364 28192-28194 IN denotes at
T12365 28195-28201 NNP denotes Ser276
T12366 28202-28205 CC denotes but
T12367 28206-28209 RB denotes not
T12368 28210-28216 NNP denotes Ser536
T12369 28216-28217 . denotes .
T12370 28218-28220 TO denotes To
T12371 28221-28228 VB denotes confirm
T12372 28229-28232 DT denotes the
T12373 28233-28237 NN denotes role
T12374 28238-28240 IN denotes of
T12375 28241-28244 NNP denotes PKC
T12376 28245-28248 CC denotes and
T12377 28249-28252 CD denotes p65
T12378 28253-28259 NNP denotes Ser276
T12379 28260-28275 NN denotes phosphorylation
T12380 28276-28278 IN denotes on
T12381 28279-28284 JJ denotes NF-κB
T12382 28285-28293 NN denotes activity
T12383 28293-28294 , denotes ,
T12384 28295-28297 PRP denotes we
T12385 28298-28312 VBD denotes co-transfected
T12386 28313-28316 DT denotes the
T12387 28317-28327 NNP denotes NF-κB-SEAP
T12388 28328-28337 VBP denotes construct
T12389 28338-28342 IN denotes with
T12390 28343-28349 DT denotes either
T12391 28350-28352 NNP denotes WT
T12392 28353-28358 NNP denotes NF-κB
T12393 28359-28362 CD denotes p65
T12394 28363-28365 CC denotes or
T12395 28366-28371 NNP denotes NF-κB
T12396 28372-28375 CD denotes p65
T12397 28376-28382 NNP denotes 276S/A
T12398 28383-28393 NNS denotes constructs
T12399 28394-28396 IN denotes in
T12400 28397-28400 NNP denotes Raw
T12401 28401-28406 CD denotes 264.7
T12402 28407-28412 NNS denotes cells
T12403 28412-28413 , denotes ,
T12404 28414-28418 RB denotes then
T12405 28419-28426 VBD denotes treated
T12406 28427-28432 NNS denotes cells
T12407 28433-28437 IN denotes with
T12408 28438-28441 DT denotes the
T12409 28442-28445 NNP denotes PKC
T12410 28446-28455 NN denotes inhibitor
T12411 28456-28466 NN denotes Ro-31-8220
T12412 28467-28469 IN denotes in
T12413 28470-28473 DT denotes the
T12414 28474-28481 NN denotes absence
T12415 28482-28484 CC denotes or
T12416 28485-28494 NNS denotes presences
T12417 28495-28497 IN denotes of
T12418 28498-28503 NNP denotes M-CSF
T12419 28503-28504 . denotes .
T12420 28505-28507 IN denotes As
T12421 28508-28516 VBN denotes expected
T12422 28517-28519 IN denotes in
T12423 28520-28525 NNS denotes cells
T12424 28526-28537 VBN denotes transfected
T12425 28538-28542 IN denotes with
T12426 28543-28549 NN denotes vector
T12427 28550-28557 NN denotes control
T12428 28557-28558 , denotes ,
T12429 28559-28569 VBG denotes inhibiting
T12430 28570-28573 NNP denotes PKC
T12431 28574-28581 VBD denotes reduced
T12432 28582-28598 JJ denotes M-CSF-stimulated
T12433 28599-28604 NN denotes NF-κB
T12434 28605-28613 NN denotes activity
T12435 28614-28622 VBN denotes compared
T12436 28623-28625 TO denotes to
T12437 28626-28631 NNS denotes cells
T12438 28632-28639 VBN denotes treated
T12439 28640-28644 IN denotes with
T12440 28645-28650 NNP denotes M-CSF
T12441 28651-28654 CC denotes and
T12442 28655-28658 DT denotes the
T12443 28659-28666 NN denotes vehicle
T12444 28667-28671 NNP denotes DMSO
T12445 28672-28673 -LRB- denotes (
T12446 28673-28674 FW denotes p
T12447 28675-28676 SYM denotes =
T12448 28677-28682 CD denotes 0.001
T12449 28682-28683 -RRB- denotes )
T12450 28684-28685 -LRB- denotes (
T12451 28685-28691 NN denotes Figure
T12452 28692-28694 NN denotes 8A
T12453 28694-28695 -RRB- denotes )
T12454 28695-28696 . denotes .
T12455 28697-28699 IN denotes In
T12456 28700-28708 NN denotes contrast
T12457 28708-28709 , denotes ,
T12458 28710-28720 VBG denotes expressing
T12459 28721-28723 NNP denotes WT
T12460 28724-28729 NNP denotes NF-κB
T12461 28730-28733 NNS denotes p65
T12462 28734-28735 -LRB- denotes (
T12463 28735-28738 CD denotes p65
T12464 28739-28741 NNP denotes WT
T12465 28741-28742 -RRB- denotes )
T12466 28743-28752 VBD denotes increased
T12467 28753-28758 JJ denotes NF-κB
T12468 28759-28767 NN denotes activity
T12469 28767-28768 , denotes ,
T12470 28769-28774 IN denotes while
T12471 28775-28778 DT denotes the
T12472 28779-28786 JJ denotes general
T12473 28787-28790 NNP denotes PKC
T12474 28791-28800 NN denotes inhibitor
T12475 28801-28811 NN denotes Ro-31-8220
T12476 28812-28821 VBD denotes decreased
T12477 28822-28826 DT denotes this
T12478 28827-28832 JJ denotes NF-κB
T12479 28833-28841 NN denotes activity
T12480 28842-28843 -LRB- denotes (
T12481 28843-28844 FW denotes p
T12482 28845-28846 SYM denotes =
T12483 28847-28852 CD denotes 0.001
T12484 28852-28853 -RRB- denotes )
T12485 28853-28854 . denotes .
T12486 28855-28862 RB denotes Notably
T12487 28862-28863 , denotes ,
T12488 28864-28867 DT denotes the
T12489 28868-28880 NN denotes introduction
T12490 28881-28883 IN denotes of
T12491 28884-28889 NNP denotes NF-κB
T12492 28890-28893 CD denotes p65
T12493 28894-28897 CD denotes 276
T12494 28898-28901 NNP denotes S/A
T12495 28902-28911 VB denotes construct
T12496 28912-28925 RB denotes significantly
T12497 28926-28933 VBN denotes reduced
T12498 28934-28939 JJ denotes NF-κB
T12499 28940-28948 NN denotes activity
T12500 28949-28957 VBN denotes compared
T12501 28958-28962 IN denotes with
T12502 28963-28966 DT denotes the
T12503 28967-28969 NNP denotes WT
T12504 28970-28975 NNP denotes NF-κB
T12505 28976-28979 NNS denotes p65
T12506 28980-28989 VBP denotes construct
T12507 28990-28991 -LRB- denotes (
T12508 28991-28992 FW denotes p
T12509 28993-28994 SYM denotes =
T12510 28995-29000 CD denotes 0.005
T12511 29000-29001 -RRB- denotes )
T12512 29002-29005 CC denotes and
T12513 29006-29011 NNP denotes M-CSF
T12514 29012-29021 NN denotes treatment
T12515 29022-29025 VBD denotes was
T12516 29026-29032 JJ denotes unable
T12517 29033-29035 TO denotes to
T12518 29036-29044 VB denotes overcome
T12519 29045-29049 DT denotes this
T12520 29050-29060 NN denotes inhibition
T12521 29060-29061 . denotes .
T26362 29106-29111 NNP denotes NF-κB
T26363 29112-29115 CD denotes p65
T26364 29116-29122 NNP denotes Ser276
T26365 29123-29125 VBZ denotes is
T26366 29126-29135 JJ denotes essential
T26367 29136-29138 IN denotes in
T26368 29139-29149 VBG denotes regulating
T26369 29150-29155 JJ denotes NF-κB
T26370 29156-29164 NN denotes activity
T26371 29164-29165 . denotes .
T26372 29166-29167 -LRB- denotes (
T26373 29167-29168 NNP denotes A
T26374 29168-29169 -RRB- denotes )
T26375 29170-29173 NNP denotes Raw
T26376 29174-29179 CD denotes 264.7
T26377 29180-29184 NN denotes cell
T26378 29185-29189 NN denotes line
T26379 29190-29193 VBD denotes was
T26380 29194-29205 RB denotes transiently
T26381 29206-29217 VBN denotes transfected
T26382 29218-29222 IN denotes with
T26383 29223-29234 JJ denotes pNF-κB-SEAP
T26384 29235-29240 IN denotes along
T26385 29241-29245 IN denotes with
T26386 29246-29251 JJ denotes empty
T26387 29252-29258 NN denotes vector
T26388 29259-29261 CC denotes or
T26389 29262-29269 NN denotes plasmid
T26390 29270-29278 VBG denotes encoding
T26391 29279-29285 CC denotes either
T26392 29286-29291 NNP denotes NF-κB
T26393 29292-29295 CD denotes p65
T26394 29296-29298 NNP denotes WT
T26395 29299-29301 CC denotes or
T26396 29302-29307 NNP denotes NF-κB
T26397 29308-29311 CD denotes p65
T26398 29312-29318 NNP denotes 276S/A
T26399 29318-29319 . denotes .
T26400 29320-29323 DT denotes The
T26401 29324-29329 NNS denotes cells
T26402 29330-29334 VBD denotes were
T26403 29335-29346 VBN denotes transfected
T26404 29347-29350 IN denotes for
T26405 29351-29356 CD denotes 18-24
T26406 29357-29362 NNS denotes hours
T26407 29362-29363 , denotes ,
T26408 29364-29369 NN denotes serum
T26409 29370-29377 VBD denotes starved
T26410 29378-29381 IN denotes for
T26411 29382-29383 CD denotes 4
T26412 29384-29389 NNS denotes hours
T26413 29389-29390 , denotes ,
T26414 29391-29394 CC denotes and
T26415 29395-29399 RB denotes then
T26416 29400-29409 VBN denotes incubated
T26417 29410-29414 IN denotes with
T26418 29415-29417 CD denotes 10
T26419 29418-29420 NN denotes µM
T26420 29421-29423 IN denotes of
T26421 29424-29434 NN denotes Ro-31-8220
T26422 29435-29438 IN denotes for
T26423 29439-29441 CD denotes 30
T26424 29442-29449 NNS denotes minutes
T26425 29450-29455 RB denotes prior
T26426 29456-29458 TO denotes to
T26427 29459-29468 NN denotes treatment
T26428 29469-29473 IN denotes with
T26429 29474-29477 CD denotes 100
T26430 29478-29483 NN denotes ng/ml
T26431 29484-29486 IN denotes of
T26432 29487-29492 NNP denotes M-CSF
T26433 29493-29496 IN denotes for
T26434 29497-29498 CD denotes 2
T26435 29499-29504 NNS denotes hours
T26436 29505-29508 CC denotes and
T26437 29509-29513 NNP denotes SEAP
T26438 29514-29523 NN denotes secretion
T26439 29524-29526 IN denotes in
T26440 29527-29530 DT denotes the
T26441 29531-29537 NN denotes medium
T26442 29538-29541 VBD denotes was
T26443 29542-29550 VBN denotes measured
T26444 29550-29551 . denotes .
T26445 29552-29553 -LRB- denotes (
T26446 29553-29554 NNP denotes B
T26447 29554-29555 -RRB- denotes )
T26448 29556-29561 NNP denotes NF-κB
T26449 29562-29565 NNS denotes p65
T26450 29565-29566 VBP denotes −
T26451 29566-29567 CD denotes /
T26452 29567-29568 CD denotes −
T26453 29569-29573 NN denotes cell
T26454 29574-29578 NN denotes line
T26455 29579-29582 VBD denotes was
T26456 29583-29594 RB denotes transiently
T26457 29595-29606 VBN denotes transfected
T26458 29607-29611 IN denotes with
T26459 29612-29623 JJ denotes pNF-κB-SEAP
T26460 29624-29629 IN denotes along
T26461 29630-29634 IN denotes with
T26462 29635-29640 JJ denotes empty
T26463 29641-29647 NN denotes vector
T26464 29648-29650 CC denotes or
T26465 29651-29658 NN denotes plasmid
T26466 29659-29667 VBG denotes encoding
T26467 29668-29674 CC denotes either
T26468 29675-29680 NNP denotes NF-κB
T26469 29681-29684 CD denotes p65
T26470 29685-29687 NNP denotes WT
T26471 29687-29688 , denotes ,
T26472 29689-29694 NNP denotes NF-κB
T26473 29695-29698 CD denotes p65
T26474 29699-29705 NNP denotes 276S/A
T26475 29705-29706 , denotes ,
T26476 29707-29709 CC denotes or
T26477 29710-29715 NNP denotes NF-κB
T26478 29716-29719 CD denotes p65
T26479 29720-29726 NNP denotes 536S/A
T26480 29726-29727 . denotes .
T26481 29728-29731 DT denotes The
T26482 29732-29737 NNS denotes cells
T26483 29738-29742 VBD denotes were
T26484 29743-29751 VBN denotes cultured
T26485 29752-29755 IN denotes for
T26486 29756-29758 CD denotes 24
T26487 29759-29764 NNS denotes hours
T26488 29765-29768 CC denotes and
T26489 29769-29773 RB denotes then
T26490 29774-29779 NN denotes serum
T26491 29780-29787 VBD denotes starved
T26492 29788-29791 IN denotes for
T26493 29792-29793 CD denotes 4
T26494 29794-29799 NNS denotes hours
T26495 29799-29800 . denotes .
T26496 29801-29806 NNS denotes Cells
T26497 29807-29811 VBD denotes were
T26498 29812-29816 RB denotes then
T26499 29817-29826 VBN denotes incubated
T26500 29827-29829 IN denotes in
T26501 29830-29835 JJ denotes fresh
T26502 29836-29840 NNP denotes DMEM
T26503 29841-29847 NN denotes medium
T26504 29848-29851 IN denotes for
T26505 29852-29853 CD denotes 2
T26506 29854-29859 NNS denotes hours
T26507 29860-29863 CC denotes and
T26508 29864-29868 NNP denotes SEAP
T26509 29869-29878 NN denotes secretion
T26510 29879-29881 IN denotes in
T26511 29882-29885 DT denotes the
T26512 29886-29892 NN denotes medium
T26513 29893-29896 VBD denotes was
T26514 29897-29905 VBN denotes measured
T26515 29905-29906 . denotes .
T26516 29907-29910 DT denotes The
T26517 29911-29918 NNS denotes results
T26518 29919-29924 VBN denotes shown
T26519 29925-29928 VBP denotes are
T26520 29929-29933 VB denotes fold
T26521 29934-29940 NN denotes change
T26522 29941-29945 IN denotes over
T26523 29946-29951 JJ denotes empty
T26524 29952-29958 NN denotes vector
T26525 29959-29960 NN denotes +
T26526 29961-29970 NN denotes pTAL-SEAP
T26527 29970-29971 . denotes .
T26528 29972-29973 -LRB- denotes (
T26529 29973-29974 NNP denotes C
T26530 29974-29975 -RRB- denotes )
T26531 29976-29981 NNP denotes NF-κB
T26532 29982-29985 NNS denotes p65
T26533 29985-29986 VBP denotes −
T26534 29986-29987 CD denotes /
T26535 29987-29988 CD denotes −
T26536 29989-29993 NN denotes cell
T26537 29994-29998 NN denotes line
T26538 29999-30002 VBD denotes was
T26539 30003-30014 RB denotes transiently
T26540 30015-30026 VBN denotes transfected
T26541 30027-30031 IN denotes with
T26542 30032-30043 JJ denotes pNF-κB-SEAP
T26543 30044-30048 IN denotes with
T26544 30049-30055 DT denotes either
T26545 30056-30061 JJ denotes empty
T26546 30062-30068 NN denotes vector
T26547 30069-30071 CC denotes or
T26548 30072-30079 NN denotes plasmid
T26549 30080-30088 VBG denotes encoding
T26550 30089-30095 CC denotes either
T26551 30096-30101 NNP denotes NF-κB
T26552 30102-30105 CD denotes p65
T26553 30106-30108 NNP denotes WT
T26554 30109-30111 CC denotes or
T26555 30112-30117 NNP denotes NF-κB
T26556 30118-30121 CD denotes p65
T26557 30122-30128 NNP denotes 276S/A
T26558 30128-30129 . denotes .
T26559 30130-30133 DT denotes The
T26560 30134-30139 NNS denotes cells
T26561 30140-30144 VBD denotes were
T26562 30145-30156 VBN denotes transfected
T26563 30157-30160 IN denotes for
T26564 30161-30163 CD denotes 24
T26565 30164-30169 NNS denotes hours
T26566 30170-30173 CC denotes and
T26567 30174-30179 NN denotes serum
T26568 30180-30187 VBD denotes starved
T26569 30188-30191 IN denotes for
T26570 30192-30193 CD denotes 4
T26571 30194-30199 NNS denotes hours
T26572 30199-30200 , denotes ,
T26573 30201-30204 CC denotes and
T26574 30205-30209 RB denotes then
T26575 30210-30219 VBN denotes incubated
T26576 30220-30224 IN denotes with
T26577 30225-30227 CD denotes 10
T26578 30228-30230 NN denotes µM
T26579 30231-30233 IN denotes of
T26580 30234-30244 NN denotes Ro-31-8220
T26581 30245-30248 IN denotes for
T26582 30249-30251 CD denotes 30
T26583 30252-30259 NNS denotes minutes
T26584 30260-30265 RB denotes prior
T26585 30266-30268 TO denotes to
T26586 30269-30278 NN denotes treatment
T26587 30279-30283 IN denotes with
T26588 30284-30286 CD denotes 10
T26589 30287-30292 NN denotes ng/ml
T26590 30293-30295 IN denotes of
T26591 30296-30300 NNP denotes TNFα
T26592 30300-30301 . denotes .
T26593 30302-30305 DT denotes The
T26594 30306-30317 JJ denotes supernatant
T26595 30318-30322 VBD denotes were
T26596 30323-30332 VBN denotes collected
T26597 30333-30338 IN denotes after
T26598 30339-30340 CD denotes 2
T26599 30341-30346 NNS denotes hours
T26600 30347-30349 IN denotes of
T26601 30350-30359 NN denotes treatment
T26602 30360-30363 CC denotes and
T26603 30364-30368 NNP denotes SEAP
T26604 30369-30378 NN denotes secretion
T26605 30379-30381 IN denotes in
T26606 30382-30385 DT denotes the
T26607 30386-30392 NN denotes medium
T26608 30393-30396 VBD denotes was
T26609 30397-30405 VBN denotes measured
T26610 30405-30406 . denotes .
T26611 30407-30410 DT denotes The
T26612 30411-30418 NNS denotes results
T26613 30419-30424 VBN denotes shown
T26614 30425-30428 VBP denotes are
T26615 30429-30432 DT denotes the
T26616 30433-30437 VBP denotes fold
T26617 30438-30444 NN denotes change
T26618 30445-30449 IN denotes over
T26619 30450-30455 JJ denotes empty
T26620 30456-30462 NN denotes vector
T26621 30463-30464 NN denotes +
T26622 30465-30476 NN denotes pNF-κB-SEAP
T26623 30476-30477 . denotes .
T26624 30478-30482 NNP denotes Data
T26625 30483-30488 VBN denotes shown
T26626 30489-30492 VBP denotes are
T26627 30493-30497 JJ denotes mean
T26628 30498-30499 NN denotes ±
T26629 30500-30505 NNP denotes S.E.M
T26630 30506-30509 IN denotes for
T26631 30510-30512 IN denotes at
T26632 30513-30518 JJS denotes least
T26633 30519-30524 CD denotes three
T26634 30525-30536 JJ denotes independent
T26635 30537-30548 NNS denotes experiments
T26636 30549-30558 VBN denotes performed
T26637 30559-30561 IN denotes in
T26638 30562-30571 VB denotes duplicate
T26639 30571-30572 . denotes .
T12522 30575-30586 RB denotes Furthermore
T12523 30586-30587 , denotes ,
T12524 30588-30590 PRP denotes we
T12525 30591-30605 VBD denotes co-transfected
T12526 30606-30609 DT denotes the
T12527 30610-30620 NNP denotes NF-κB-SEAP
T12528 30621-30630 VBP denotes construct
T12529 30631-30636 IN denotes along
T12530 30637-30641 IN denotes with
T12531 30642-30648 CC denotes either
T12532 30649-30652 DT denotes the
T12533 30653-30655 NNP denotes WT
T12534 30656-30661 NNP denotes NF-κB
T12535 30662-30665 NNS denotes p65
T12536 30665-30666 , denotes ,
T12537 30667-30672 NNP denotes NF-κB
T12538 30673-30676 CD denotes p65
T12539 30677-30683 NNP denotes 276S/A
T12540 30684-30686 CC denotes or
T12541 30687-30692 NNP denotes NF-κB
T12542 30693-30696 CD denotes p65
T12543 30697-30703 NNP denotes 536S/A
T12544 30704-30714 NNS denotes constructs
T12545 30715-30719 IN denotes into
T12546 30720-30721 DT denotes a
T12547 30722-30727 NNP denotes NF-κB
T12548 30728-30731 CD denotes p65
T12549 30731-30732 FW denotes −
T12550 30732-30733 FW denotes /
T12551 30733-30734 FW denotes −
T12552 30735-30741 FW denotes murine
T12553 30742-30752 FW denotes fibroblast
T12554 30753-30757 NN denotes cell
T12555 30758-30762 NN denotes line
T12556 30763-30766 CC denotes and
T12557 30767-30775 VBN denotes measured
T12558 30776-30781 JJ denotes NF-κB
T12559 30782-30790 NN denotes activity
T12560 30790-30791 . denotes .
T12561 30792-30794 IN denotes As
T12562 30795-30804 VBN denotes predicted
T12563 30804-30805 , denotes ,
T12564 30806-30816 NN denotes expression
T12565 30817-30819 IN denotes of
T12566 30820-30822 NNP denotes WT
T12567 30823-30828 NNP denotes NF-κB
T12568 30829-30832 NNS denotes p65
T12569 30833-30834 -LRB- denotes (
T12570 30834-30837 CD denotes p65
T12571 30838-30840 NNP denotes WT
T12572 30840-30841 -RRB- denotes )
T12573 30842-30844 IN denotes in
T12574 30845-30850 NNP denotes NF-κB
T12575 30851-30854 CD denotes p65
T12576 30854-30855 CD denotes −
T12577 30855-30856 NN denotes /
T12578 30856-30857 NN denotes −
T12579 30858-30862 NN denotes cell
T12580 30863-30867 NN denotes line
T12581 30868-30882 RB denotes constitutively
T12582 30883-30892 VBD denotes activated
T12583 30893-30898 NNP denotes NF-κB
T12584 30899-30907 VBN denotes compared
T12585 30908-30910 TO denotes to
T12586 30911-30916 NNS denotes cells
T12587 30917-30928 VBN denotes transfected
T12588 30929-30933 IN denotes with
T12589 30934-30940 NN denotes vector
T12590 30941-30948 NN denotes control
T12591 30949-30956 IN denotes without
T12592 30957-30960 DT denotes any
T12593 30961-30972 NN denotes stimulation
T12594 30973-30974 -LRB- denotes (
T12595 30974-30980 NN denotes Figure
T12596 30981-30983 NN denotes 8B
T12597 30983-30984 -RRB- denotes )
T12598 30984-30985 . denotes .
T12599 30986-30988 IN denotes In
T12600 30989-30999 NN denotes comparison
T12601 30999-31000 , denotes ,
T12602 31001-31013 VBG denotes transfecting
T12603 31014-31017 DT denotes the
T12604 31018-31023 NNP denotes NF-κB
T12605 31024-31027 CD denotes p65
T12606 31028-31034 NNP denotes 276S/A
T12607 31035-31044 VB denotes construct
T12608 31045-31052 VBN denotes reduced
T12609 31053-31058 JJ denotes NF-κB
T12610 31059-31067 NN denotes activity
T12611 31068-31070 IN denotes by
T12612 31071-31077 JJ denotes 5-fold
T12613 31078-31079 -LRB- denotes (
T12614 31079-31080 FW denotes p
T12615 31081-31082 SYM denotes =
T12616 31083-31088 CD denotes 0.006
T12617 31088-31089 , denotes ,
T12618 31090-31092 NNP denotes WT
T12619 31093-31098 NNP denotes NF-κB
T12620 31099-31102 CD denotes p65
T12621 31103-31106 FW denotes vs.
T12622 31107-31112 NNP denotes NF-κB
T12623 31113-31116 CD denotes p65
T12624 31117-31123 NNP denotes 276S/A
T12625 31123-31124 -RRB- denotes )
T12626 31124-31125 , denotes ,
T12627 31126-31131 IN denotes while
T12628 31132-31142 VBG denotes expressing
T12629 31143-31146 DT denotes the
T12630 31147-31152 NNP denotes NF-κB
T12631 31153-31156 CD denotes p65
T12632 31157-31163 NNP denotes 536S/A
T12633 31164-31173 VB denotes construct
T12634 31174-31183 VBN denotes increased
T12635 31184-31189 JJ denotes NF-κB
T12636 31190-31198 NN denotes activity
T12637 31199-31201 IN denotes in
T12638 31202-31205 DT denotes the
T12639 31206-31209 NNS denotes p65
T12640 31209-31210 VBP denotes −
T12641 31210-31211 CD denotes /
T12642 31211-31212 JJ denotes −
T12643 31213-31218 NNS denotes cells
T12644 31219-31221 TO denotes to
T12645 31222-31228 NNS denotes levels
T12646 31229-31236 JJ denotes similar
T12647 31237-31239 TO denotes to
T12648 31240-31242 NNP denotes WT
T12649 31243-31248 NNP denotes NF-κB
T12650 31249-31252 CD denotes p65
T12651 31253-31264 VBD denotes transfected
T12652 31265-31270 NNS denotes cells
T12653 31270-31271 . denotes .
T12654 31272-31277 IN denotes Since
T12655 31278-31291 JJ denotes M-CSF-induced
T12656 31292-31295 NNP denotes PKC
T12657 31296-31306 NN denotes activation
T12658 31307-31316 VBN denotes regulated
T12659 31317-31322 JJ denotes NF-κB
T12660 31323-31331 NN denotes activity
T12661 31332-31335 IN denotes via
T12662 31336-31342 NNP denotes Ser276
T12663 31343-31350 NN denotes residue
T12664 31351-31353 IN denotes of
T12665 31354-31359 NNP denotes NF-κB
T12666 31360-31363 CD denotes p65
T12667 31364-31366 IN denotes in
T12668 31367-31374 JJ denotes primary
T12669 31375-31380 JJ denotes human
T12670 31381-31385 NNS denotes MDMs
T12671 31386-31389 CC denotes and
T12672 31390-31393 NNP denotes RAW
T12673 31394-31399 CD denotes 264.7
T12674 31400-31405 NNS denotes cells
T12675 31405-31406 , denotes ,
T12676 31407-31409 PRP denotes we
T12677 31410-31414 JJ denotes next
T12678 31415-31423 VBN denotes examined
T12679 31424-31426 IN denotes if
T12680 31427-31431 DT denotes this
T12681 31432-31440 VBD denotes occurred
T12682 31441-31443 IN denotes in
T12683 31444-31449 NNP denotes NF-κB
T12684 31450-31453 CD denotes p65
T12685 31453-31454 CD denotes −
T12686 31454-31455 NN denotes /
T12687 31455-31456 NN denotes −
T12688 31457-31468 NNS denotes fibroblasts
T12689 31469-31471 IN denotes in
T12690 31472-31480 NN denotes response
T12691 31481-31483 TO denotes to
T12692 31484-31485 DT denotes a
T12693 31486-31492 JJ denotes native
T12694 31493-31501 NN denotes stimulus
T12695 31502-31505 IN denotes for
T12696 31506-31511 DT denotes these
T12697 31512-31517 NNS denotes cells
T12698 31517-31518 , denotes ,
T12699 31519-31523 NNP denotes TNFα
T12700 31523-31524 . denotes .
T12701 31525-31530 JJ denotes NF-κB
T12702 31531-31539 NN denotes activity
T12703 31540-31543 VBD denotes was
T12704 31544-31552 VBN denotes measured
T12705 31553-31555 IN denotes in
T12706 31556-31559 DT denotes the
T12707 31560-31562 NNP denotes WT
T12708 31563-31568 NNP denotes NF-κB
T12709 31569-31572 CD denotes p65
T12710 31573-31575 CC denotes or
T12711 31576-31581 NNP denotes NF-κB
T12712 31582-31585 CD denotes p65
T12713 31586-31592 NNP denotes 276S/A
T12714 31593-31604 VBD denotes transfected
T12715 31605-31610 NNS denotes cells
T12716 31611-31618 VBN denotes treated
T12717 31619-31623 IN denotes with
T12718 31624-31628 NNP denotes TNFα
T12719 31629-31631 IN denotes in
T12720 31632-31635 DT denotes the
T12721 31636-31643 NN denotes absence
T12722 31644-31646 CC denotes or
T12723 31647-31655 NN denotes presence
T12724 31656-31658 IN denotes of
T12725 31659-31669 NNP denotes Ro-31-8220
T12726 31670-31671 -LRB- denotes (
T12727 31671-31677 NNP denotes Figure
T12728 31678-31680 NNP denotes 8C
T12729 31680-31681 -RRB- denotes )
T12730 31681-31682 . denotes .
T12731 31683-31685 IN denotes As
T12732 31686-31694 VBN denotes expected
T12733 31694-31695 , denotes ,
T12734 31696-31700 NNP denotes TNFα
T12735 31701-31710 VBD denotes increased
T12736 31711-31716 JJ denotes NF-κB
T12737 31717-31725 NN denotes activity
T12738 31726-31728 IN denotes in
T12739 31729-31734 NNP denotes NF-κB
T12740 31735-31738 CD denotes p65
T12741 31738-31739 CD denotes −
T12742 31739-31740 NN denotes /
T12743 31740-31741 NN denotes −
T12744 31742-31747 NNS denotes cells
T12745 31748-31758 VBG denotes expressing
T12746 31759-31761 NNP denotes WT
T12747 31762-31765 CD denotes p65
T12748 31766-31769 CC denotes and
T12749 31770-31771 -LRB- denotes (
T12750 31771-31772 FW denotes p
T12751 31773-31774 SYM denotes =
T12752 31775-31780 CD denotes 0.001
T12753 31780-31781 -RRB- denotes )
T12754 31782-31785 NNP denotes PKC
T12755 31786-31796 NNS denotes inhibitors
T12756 31797-31806 VBD denotes decreased
T12757 31807-31812 JJ denotes NF-κB
T12758 31813-31821 NN denotes activity
T12759 31822-31824 TO denotes to
T12760 31825-31828 DT denotes the
T12761 31829-31843 JJ denotes non-stimulated
T12762 31844-31845 -LRB- denotes (
T12763 31845-31847 NNP denotes NS
T12764 31847-31848 -RRB- denotes )
T12765 31849-31854 NN denotes level
T12766 31855-31856 -LRB- denotes (
T12767 31856-31857 FW denotes p
T12768 31858-31859 SYM denotes =
T12769 31860-31865 CD denotes 0.007
T12770 31865-31866 -RRB- denotes )
T12771 31866-31867 . denotes .
T12772 31868-31879 VBG denotes Introducing
T12773 31880-31885 JJ denotes NF-κB
T12774 31886-31889 NNS denotes p65
T12775 31890-31893 CD denotes 276
T12776 31894-31897 NNP denotes S/A
T12777 31898-31908 NNS denotes constructs
T12778 31909-31922 RB denotes significantly
T12779 31923-31930 VBD denotes reduced
T12780 31931-31936 JJ denotes NF-κB
T12781 31937-31945 NN denotes activity
T12782 31946-31954 VBN denotes compared
T12783 31955-31959 IN denotes with
T12784 31960-31963 DT denotes the
T12785 31964-31966 NNP denotes WT
T12786 31967-31972 NNP denotes NF-κB
T12787 31973-31976 NNS denotes p65
T12788 31977-31986 VBP denotes construct
T12789 31987-31988 -LRB- denotes (
T12790 31988-31989 FW denotes p
T12791 31990-31991 SYM denotes =
T12792 31992-31997 CD denotes 0.001
T12793 31997-31998 -RRB- denotes )
T12794 31998-31999 . denotes .
T12795 32000-32007 RB denotes Notably
T12796 32007-32008 , denotes ,
T12797 32009-32013 NNP denotes TNFα
T12798 32014-32020 VBD denotes failed
T12799 32021-32023 TO denotes to
T12800 32024-32032 VB denotes increase
T12801 32033-32038 JJ denotes NF-κB
T12802 32039-32047 NN denotes activity
T12803 32048-32050 IN denotes in
T12804 32051-32054 DT denotes the
T12805 32055-32060 NNP denotes NF-κB
T12806 32061-32064 CD denotes p65
T12807 32064-32065 CD denotes −
T12808 32065-32066 NN denotes /
T12809 32066-32067 NN denotes −
T12810 32068-32073 NNS denotes cells
T12811 32074-32084 VBG denotes expressing
T12812 32085-32090 NNP denotes NF-κB
T12813 32091-32094 CD denotes p65
T12814 32095-32101 NNP denotes 276S/A
T12815 32102-32112 NNS denotes constructs
T12816 32112-32113 . denotes .
T12817 32114-32119 DT denotes These
T12818 32120-32132 NNS denotes observations
T12819 32133-32136 VBP denotes are
T12820 32137-32144 JJ denotes similar
T12821 32145-32147 TO denotes to
T12822 32148-32159 NNS denotes macrophages
T12823 32160-32174 VBG denotes overexpressing
T12824 32175-32178 DT denotes the
T12825 32179-32184 NNP denotes NF-κB
T12826 32185-32188 CD denotes p65
T12827 32189-32195 NNP denotes 276S/A
T12828 32196-32197 -LRB- denotes (
T12829 32197-32203 NNP denotes Figure
T12830 32204-32206 NNP denotes 8A
T12831 32206-32207 -RRB- denotes )
T12832 32207-32208 . denotes .
T12833 32209-32212 PRP$ denotes Our
T12834 32213-32217 NNS denotes data
T12835 32218-32229 VBP denotes demonstrate
T12836 32230-32234 IN denotes that
T12837 32235-32241 NNP denotes Ser276
T12838 32242-32244 IN denotes of
T12839 32245-32250 NNP denotes NF-κB
T12840 32251-32254 CD denotes p65
T12841 32255-32257 VBZ denotes is
T12842 32258-32267 JJ denotes essential
T12843 32268-32270 IN denotes in
T12844 32271-32281 VBG denotes regulating
T12845 32282-32287 JJ denotes NF-κB
T12846 32288-32296 NN denotes activity
T12847 32297-32300 CC denotes and
T12848 32301-32309 VBZ denotes suggests
T12849 32310-32314 IN denotes that
T12850 32315-32318 NNP denotes PKC
T12851 32319-32328 VBZ denotes regulates
T12852 32329-32334 JJ denotes NF-κB
T12853 32335-32343 NN denotes activity
T12854 32344-32346 IN denotes by
T12855 32347-32357 VBG denotes modulating
T12856 32358-32361 DT denotes the
T12857 32362-32377 NN denotes phosphorylation
T12858 32378-32380 IN denotes of
T12859 32381-32386 NNP denotes NF-κB
T12860 32387-32390 NNS denotes p65
T12861 32391-32393 IN denotes at
T12862 32394-32400 NNP denotes Ser276
T12863 32401-32408 NN denotes residue
T12864 32408-32409 . denotes .
T14525 32469-32479 VBG denotes regulating
T14526 32480-32493 JJ denotes intracellular
T14527 32494-32503 VBG denotes signaling
T14528 32503-32504 , denotes ,
T14529 32505-32511 NN denotes stress
T14530 32512-32520 NN denotes response
T14531 32520-32521 , denotes ,
T14532 32522-32535 NN denotes proliferation
T14533 32535-32536 , denotes ,
T14534 32537-32545 NN denotes survival
T14535 32545-32546 , denotes ,
T14536 32547-32562 NN denotes differentiation
T14537 32563-32566 CC denotes and
T14538 32567-32579 NN denotes inflammation
T14539 32580-32581 NN denotes [
T14540 32581-32582 CD denotes 8
T14541 32582-32583 NNP denotes ]
T14542 32583-32584 . denotes .
T14543 32585-32587 TO denotes To
T14544 32588-32596 VB denotes regulate
T14545 32597-32601 NN denotes gene
T14546 32602-32615 NN denotes transcription
T14547 32615-32616 , denotes ,
T14548 32617-32622 NNP denotes NF-κB
T14549 32623-32630 CD denotes p50/p65
T14550 32631-32643 NNS denotes heterodimers
T14551 32644-32655 VBP denotes translocate
T14552 32656-32658 TO denotes to
T14553 32659-32662 DT denotes the
T14554 32663-32670 NN denotes nucleus
T14555 32671-32675 IN denotes from
T14556 32676-32679 DT denotes the
T14557 32680-32689 NN denotes cytoplasm
T14558 32689-32690 , denotes ,
T14559 32691-32698 RB denotes however
T14560 32698-32699 , denotes ,
T14561 32700-32705 PRP$ denotes their
T14562 32706-32719 NN denotes translocation
T14563 32720-32722 VBZ denotes is
T14564 32723-32726 RB denotes not
T14565 32727-32737 JJ denotes sufficient
T14566 32738-32740 TO denotes to
T14567 32741-32749 VB denotes activate
T14568 32750-32754 NN denotes gene
T14569 32755-32768 NN denotes transcription
T14570 32768-32769 . denotes .
T14571 32770-32788 JJ denotes Post-translational
T14572 32789-32801 NN denotes modification
T14573 32802-32804 IN denotes of
T14574 32805-32810 JJ denotes NF-κB
T14575 32811-32823 NNS denotes heterodimers
T14576 32824-32828 JJ denotes such
T14577 32829-32831 IN denotes as
T14578 32832-32847 NN denotes phosphorylation
T14579 32848-32850 CC denotes or
T14580 32851-32862 NN denotes acetylation
T14581 32863-32867 RB denotes also
T14582 32868-32879 VBZ denotes contributes
T14583 32880-32882 TO denotes to
T14584 32883-32886 PRP$ denotes its
T14585 32887-32902 JJ denotes transcriptional
T14586 32903-32911 NN denotes activity
T14587 32912-32913 NNP denotes [
T14588 32913-32915 CD denotes 41
T14589 32915-32916 NNP denotes ]
T14590 32916-32917 , denotes ,
T14591 32918-32919 NNP denotes [
T14592 32919-32921 CD denotes 42
T14593 32921-32922 NNP denotes ]
T14594 32922-32923 , denotes ,
T14595 32924-32925 NNP denotes [
T14596 32925-32927 CD denotes 43
T14597 32927-32928 NNP denotes ]
T14598 32928-32929 . denotes .
T14599 32930-32937 IN denotes Because
T14600 32938-32943 JJ denotes NF-κB
T14601 32944-32958 RB denotes constitutively
T14602 32959-32971 VBZ denotes translocates
T14603 32972-32974 TO denotes to
T14604 32975-32978 DT denotes the
T14605 32979-32986 NN denotes nucleus
T14606 32987-32989 IN denotes in
T14607 32990-32999 JJ denotes monocytic
T14608 33000-33007 NN denotes lineage
T14609 33008-33009 NNP denotes [
T14610 33009-33011 CD denotes 13
T14611 33011-33012 NNP denotes ]
T14612 33013-33016 CC denotes and
T14613 33017-33026 VBZ denotes activates
T14614 33027-33031 NN denotes gene
T14615 33032-33045 NN denotes transcription
T14616 33046-33049 CC denotes and
T14617 33050-33058 NN denotes survival
T14618 33059-33061 IN denotes in
T14619 33062-33068 JJ denotes murine
T14620 33069-33080 NNS denotes macrophages
T14621 33081-33082 NNP denotes [
T14622 33082-33084 CD denotes 11
T14623 33084-33085 NNP denotes ]
T14624 33085-33086 , denotes ,
T14625 33087-33089 PRP denotes we
T14626 33090-33096 VBD denotes sought
T14627 33097-33099 TO denotes to
T14628 33100-33109 VB denotes determine
T14629 33110-33113 WRB denotes how
T14630 33114-33119 JJ denotes M-CSF
T14631 33120-33128 JJ denotes affected
T14632 33129-33134 NN denotes NF-κB
T14633 33135-33150 JJ denotes transcriptional
T14634 33151-33159 NN denotes activity
T14635 33160-33162 IN denotes in
T14636 33163-33168 JJ denotes human
T14637 33169-33173 NNS denotes MDMs
T14638 33174-33177 CC denotes and
T14639 33178-33185 IN denotes whether
T14640 33186-33190 DT denotes this
T14641 33191-33201 NN denotes activation
T14642 33202-33211 VBN denotes regulated
T14643 33212-33217 PRP$ denotes their
T14644 33218-33226 NN denotes survival
T14645 33226-33227 . denotes .
T14646 33228-33231 DT denotes The
T14647 33232-33239 JJ denotes present
T14648 33240-33245 NN denotes study
T14649 33246-33258 VBD denotes demonstrated
T14650 33259-33263 IN denotes that
T14651 33264-33269 NNP denotes M-CSF
T14652 33270-33280 VBD denotes stimulated
T14653 33281-33285 NNP denotes PKCα
T14654 33286-33292 NN denotes kinase
T14655 33293-33301 RB denotes upstream
T14656 33302-33304 IN denotes of
T14657 33305-33310 JJ denotes NF-κB
T14658 33311-33326 JJ denotes transcriptional
T14659 33327-33335 NN denotes activity
T14660 33336-33338 IN denotes in
T14661 33339-33346 JJ denotes primary
T14662 33347-33352 JJ denotes human
T14663 33353-33357 NNS denotes MDMs
T14664 33358-33361 CC denotes and
T14665 33362-33365 NNP denotes RAW
T14666 33366-33371 CD denotes 264.7
T14667 33372-33377 NNS denotes cells
T14668 33377-33378 . denotes .
T14669 33379-33381 PRP denotes We
T14670 33382-33388 VBD denotes showed
T14671 33389-33399 NN denotes inhibition
T14672 33400-33402 IN denotes of
T14673 33403-33415 JJ denotes conventional
T14674 33416-33420 NNS denotes PKCs
T14675 33421-33423 IN denotes by
T14676 33424-33427 NNP denotes PKC
T14677 33428-33438 NNS denotes inhibitors
T14678 33438-33439 , denotes ,
T14679 33440-33446 NN denotes kinase
T14680 33447-33456 NN denotes deficient
T14681 33457-33461 NNP denotes PKCα
T14682 33462-33464 CC denotes or
T14683 33465-33469 NNP denotes PKCα
T14684 33470-33475 NNP denotes siRNA
T14685 33476-33483 VBD denotes blocked
T14686 33484-33497 JJ denotes M-CSF-induced
T14687 33498-33502 NN denotes cell
T14688 33503-33511 NN denotes survival
T14689 33512-33515 CC denotes and
T14690 33516-33531 JJ denotes NF-κB-regulated
T14691 33532-33536 NN denotes gene
T14692 33537-33547 NN denotes expression
T14693 33547-33548 . denotes .
T14694 33549-33553 DT denotes This
T14695 33554-33559 NN denotes block
T14696 33560-33570 VBN denotes correlated
T14697 33571-33575 IN denotes with
T14698 33576-33577 DT denotes a
T14699 33578-33587 NN denotes reduction
T14700 33588-33590 IN denotes in
T14701 33591-33604 JJ denotes M-CSF-induced
T14702 33605-33620 NN denotes phosphorylation
T14703 33621-33623 IN denotes of
T14704 33624-33629 NNP denotes NF-κB
T14705 33630-33633 NNS denotes p65
T14706 33634-33636 IN denotes at
T14707 33637-33643 NNP denotes Ser276
T14708 33643-33644 . denotes .
T14709 33645-33655 JJ denotes Consistent
T14710 33656-33660 IN denotes with
T14711 33661-33666 DT denotes these
T14712 33667-33675 NNS denotes findings
T14713 33676-33679 DT denotes the
T14714 33680-33688 JJ denotes dominant
T14715 33689-33697 JJ denotes negative
T14716 33698-33702 NNP denotes PKCα
T14717 33703-33713 NNS denotes constructs
T14718 33714-33718 RB denotes also
T14719 33719-33728 VBD denotes inhibited
T14720 33729-33734 NNP denotes NF-κB
T14721 33735-33738 CD denotes p65
T14722 33739-33754 NN denotes phosphorylation
T14723 33755-33757 IN denotes at
T14724 33758-33764 NNP denotes Ser276
T14725 33765-33768 CC denotes but
T14726 33769-33772 RB denotes not
T14727 33773-33775 IN denotes at
T14728 33776-33782 CD denotes Ser536
T14729 33783-33792 VBG denotes resulting
T14730 33793-33795 IN denotes in
T14731 33796-33803 VBN denotes reduced
T14732 33804-33809 NNP denotes NF-κB
T14733 33810-33825 JJ denotes transcriptional
T14734 33826-33836 NN denotes activation
T14735 33837-33840 CC denotes and
T14736 33841-33854 JJ denotes M-CSF-induced
T14737 33855-33858 NNP denotes MDM
T14738 33859-33867 NN denotes survival
T14739 33867-33868 . denotes .
T14740 33869-33870 DT denotes A
T14741 33871-33881 VBN denotes simplified
T14742 33882-33890 VBN denotes proposed
T14743 33891-33898 NN denotes cartoon
T14744 33899-33904 NN denotes model
T14745 33905-33907 IN denotes in
T14746 33908-33914 NN denotes Figure
T14747 33915-33916 CD denotes 9
T14748 33917-33929 VBZ denotes demonstrates
T14749 33930-33934 IN denotes that
T14750 33935-33940 NNP denotes M-CSF
T14751 33941-33948 VBD denotes induced
T14752 33949-33958 NNS denotes monocytes
T14753 33959-33967 NN denotes survival
T14754 33968-33970 VBZ denotes is
T14755 33971-33980 VBN denotes regulated
T14756 33981-33983 IN denotes by
T14757 33984-33994 VBG denotes activating
T14758 33995-34000 NNP denotes NF-κB
T14759 34001-34004 CD denotes p65
T14760 34005-34020 NN denotes phosphorylation
T14761 34021-34023 IN denotes at
T14762 34024-34030 NNP denotes Ser276
T14763 34031-34034 IN denotes via
T14764 34035-34038 NNP denotes PKC
T14765 34038-34039 . denotes .
T14766 34040-34047 RB denotes Finally
T14767 34047-34048 , denotes ,
T14768 34049-34051 IN denotes in
T14769 34052-34053 DT denotes a
T14770 34054-34059 NNP denotes NF-κB
T14771 34060-34063 CD denotes p65
T14772 34063-34064 CD denotes −
T14773 34064-34065 NN denotes /
T14774 34065-34066 NN denotes −
T14775 34067-34077 NN denotes fibroblast
T14776 34078-34082 NN denotes cell
T14777 34083-34087 NN denotes line
T14778 34087-34088 , denotes ,
T14779 34089-34091 PRP denotes we
T14780 34092-34101 VBD denotes confirmed
T14781 34102-34106 IN denotes that
T14782 34107-34110 DT denotes the
T14783 34111-34117 NNP denotes Ser276
T14784 34118-34125 NN denotes residue
T14785 34126-34128 IN denotes of
T14786 34129-34134 NNP denotes NF-κB
T14787 34135-34138 CD denotes p65
T14788 34139-34141 VBZ denotes is
T14789 34142-34151 JJ denotes important
T14790 34152-34155 CC denotes and
T14791 34156-34165 JJ denotes essential
T14792 34166-34169 IN denotes for
T14793 34170-34173 NNP denotes PKC
T14794 34174-34184 NN denotes modulation
T14795 34185-34187 IN denotes of
T14796 34188-34193 JJ denotes NF-κB
T14797 34194-34202 NN denotes activity
T14798 34202-34203 . denotes .
T14799 34204-34208 RBR denotes More
T14800 34209-34219 JJ denotes compelling
T14801 34220-34222 VBZ denotes is
T14802 34223-34227 IN denotes that
T14803 34228-34230 PRP denotes we
T14804 34231-34239 VBD denotes observed
T14805 34240-34241 DT denotes a
T14806 34242-34249 JJ denotes similar
T14807 34250-34260 NN denotes regulation
T14808 34261-34268 IN denotes between
T14809 34269-34272 CD denotes two
T14810 34273-34282 JJ denotes different
T14811 34283-34287 NN denotes cell
T14812 34288-34296 NNS denotes lineages
T14813 34297-34299 IN denotes in
T14814 34300-34303 PRP$ denotes our
T14815 34304-34309 NN denotes study
T14816 34309-34310 . denotes .
T26848 34355-34363 VBN denotes Proposed
T26849 34364-34369 NN denotes model
T26850 34370-34373 IN denotes for
T26851 34374-34387 JJ denotes M-CSF-induced
T26852 34388-34396 NN denotes monocyte
T26853 34397-34405 NN denotes survival
T26854 34406-34409 IN denotes via
T26855 34410-34413 NNP denotes PKC
T26856 34414-34424 NN denotes regulation
T26857 34425-34427 IN denotes by
T26858 34428-34438 VBG denotes activating
T26859 34439-34444 NNP denotes NF-κB
T26860 34445-34448 CD denotes p65
T26861 34449-34464 NN denotes phosphorylation
T26862 34465-34467 IN denotes at
T26863 34468-34474 NNP denotes Ser276
T26864 34474-34475 . denotes .
T14817 34478-34496 JJ denotes Post-translational
T14818 34497-34509 NN denotes modification
T14819 34510-34512 IN denotes of
T14820 34513-34518 NNP denotes NF-κB
T14821 34519-34522 CD denotes p65
T14822 34523-34532 VBZ denotes regulates
T14823 34533-34536 PRP$ denotes its
T14824 34537-34547 NN denotes activating
T14825 34548-34550 CC denotes or
T14826 34551-34561 VBG denotes repressing
T14827 34562-34569 NNS denotes effects
T14828 34570-34572 IN denotes on
T14829 34573-34577 NN denotes gene
T14830 34578-34588 NN denotes expression
T14831 34588-34589 . denotes .
T14832 34590-34595 IN denotes Among
T14833 34596-34599 DT denotes the
T14834 34600-34605 CD denotes seven
T14835 34606-34614 VBD denotes reported
T14836 34615-34623 JJ denotes putative
T14837 34624-34629 NNP denotes NF-κB
T14838 34630-34633 CD denotes p65
T14839 34634-34649 NN denotes phosphorylation
T14840 34650-34655 NNS denotes sites
T14841 34655-34656 , denotes ,
T14842 34657-34661 CD denotes five
T14843 34662-34670 NN denotes increase
T14844 34671-34678 JJ denotes nuclear
T14845 34679-34692 NN denotes translocation
T14846 34692-34693 , denotes ,
T14847 34694-34697 NNP denotes DNA
T14848 34698-34705 JJ denotes binding
T14849 34706-34709 CC denotes and
T14850 34710-34715 JJ denotes NF-κB
T14851 34716-34731 JJ denotes transcriptional
T14852 34732-34740 NN denotes activity
T14853 34741-34742 NNP denotes [
T14854 34742-34744 CD denotes 17
T14855 34744-34745 NNP denotes ]
T14856 34745-34746 . denotes .
T14857 34747-34754 JJ denotes Similar
T14858 34755-34757 TO denotes to
T14859 34758-34761 PRP$ denotes our
T14860 34762-34769 NN denotes finding
T14861 34769-34770 , denotes ,
T14862 34771-34776 NNP denotes Zhong
T14863 34777-34779 FW denotes et
T14864 34780-34783 FW denotes al.
T14865 34784-34789 VBD denotes found
T14866 34790-34794 IN denotes that
T14867 34795-34797 IN denotes in
T14868 34798-34806 NN denotes response
T14869 34807-34809 TO denotes to
T14870 34810-34813 NNP denotes LPS
T14871 34813-34814 , denotes ,
T14872 34815-34820 NNP denotes NF-κB
T14873 34821-34824 CD denotes p65
T14874 34825-34827 VBZ denotes is
T14875 34828-34842 VBN denotes phosphorylated
T14876 34843-34845 IN denotes at
T14877 34846-34849 DT denotes the
T14878 34850-34856 RB denotes highly
T14879 34857-34866 VBN denotes conserved
T14880 34867-34873 NNP denotes Ser276
T14881 34874-34881 NN denotes residue
T14882 34882-34884 IN denotes by
T14883 34885-34888 DT denotes the
T14884 34889-34898 JJ denotes catalytic
T14885 34899-34906 NN denotes subunit
T14886 34907-34909 IN denotes of
T14887 34910-34913 NNP denotes PKA
T14888 34914-34915 NNP denotes [
T14889 34915-34917 CD denotes 16
T14890 34917-34918 NNP denotes ]
T14891 34918-34919 . denotes .
T14892 34920-34922 IN denotes In
T14893 34923-34931 NN denotes addition
T14894 34932-34934 TO denotes to
T14895 34935-34938 NNP denotes PKA
T14896 34938-34939 , denotes ,
T14897 34940-34946 NNP denotes Ser276
T14898 34947-34950 MD denotes can
T14899 34951-34953 VB denotes be
T14900 34954-34968 VBN denotes phosphorylated
T14901 34969-34971 IN denotes by
T14902 34972-34976 NNP denotes MSK1
T14903 34977-34979 IN denotes in
T14904 34980-34983 DT denotes the
T14905 34984-34991 NN denotes nucleus
T14906 34992-34996 IN denotes upon
T14907 34997-35001 NNP denotes TNFα
T14908 35002-35011 NN denotes treatment
T14909 35012-35013 NNP denotes [
T14910 35013-35015 CD denotes 18
T14911 35015-35016 NNP denotes ]
T14912 35016-35017 . denotes .
T14913 35018-35033 NNP denotes Phosphorylation
T14914 35034-35036 IN denotes of
T14915 35037-35042 NNP denotes NF-κB
T14916 35043-35046 NNS denotes p65
T14917 35047-35049 IN denotes at
T14918 35050-35056 NNP denotes Ser536
T14919 35057-35063 VBZ denotes occurs
T14920 35064-35066 IN denotes in
T14921 35067-35075 NN denotes response
T14922 35076-35078 TO denotes to
T14923 35079-35083 JJ denotes many
T14924 35084-35096 JJ denotes inflammatory
T14925 35097-35104 NNS denotes stimuli
T14926 35105-35107 RB denotes as
T14927 35108-35112 RB denotes well
T14928 35113-35115 IN denotes as
T14929 35116-35123 NNS denotes kinases
T14930 35123-35124 , denotes ,
T14931 35125-35129 NNP denotes IKKα
T14932 35129-35130 , denotes ,
T14933 35131-35135 NNP denotes IKKβ
T14934 35136-35139 CC denotes and
T14935 35140-35144 NNP denotes RSK1
T14936 35145-35146 NNP denotes [
T14937 35146-35148 CD denotes 17
T14938 35148-35149 NNP denotes ]
T14939 35149-35150 , denotes ,
T14940 35151-35152 NNP denotes [
T14941 35152-35154 CD denotes 18
T14942 35154-35155 NNP denotes ]
T14943 35155-35156 , denotes ,
T14944 35157-35158 NNP denotes [
T14945 35158-35160 CD denotes 19
T14946 35160-35161 NNP denotes ]
T14947 35161-35162 , denotes ,
T14948 35163-35164 NNP denotes [
T14949 35164-35166 CD denotes 20
T14950 35166-35167 NNP denotes ]
T14951 35167-35168 , denotes ,
T14952 35169-35170 NNP denotes [
T14953 35170-35172 CD denotes 21
T14954 35172-35173 NNP denotes ]
T14955 35173-35174 . denotes .
T14956 35175-35182 RB denotes Notably
T14957 35182-35183 , denotes ,
T14958 35184-35186 IN denotes in
T14959 35187-35195 NN denotes addition
T14960 35196-35198 TO denotes to
T14961 35199-35214 VBG denotes phosphorylating
T14962 35215-35221 NNP denotes Ser276
T14963 35222-35224 IN denotes of
T14964 35225-35230 NNP denotes NF-κB
T14965 35230-35231 , denotes ,
T14966 35232-35235 PRP$ denotes our
T14967 35236-35241 NN denotes study
T14968 35242-35250 VBD denotes revealed
T14969 35251-35255 IN denotes that
T14970 35256-35261 NNP denotes M-CSF
T14971 35262-35266 RB denotes also
T14972 35267-35276 VBD denotes modulated
T14973 35277-35280 DT denotes the
T14974 35281-35296 NN denotes phosphorylation
T14975 35297-35299 IN denotes of
T14976 35300-35303 DT denotes the
T14977 35304-35309 NNP denotes NF-κB
T14978 35310-35313 CD denotes p65
T14979 35314-35321 NN denotes subunit
T14980 35322-35324 IN denotes at
T14981 35325-35331 NNP denotes Ser536
T14982 35331-35332 , denotes ,
T14983 35333-35340 RB denotes however
T14984 35340-35341 , denotes ,
T14985 35342-35345 NNP denotes PKC
T14986 35346-35356 NNS denotes inhibitors
T14987 35357-35359 CC denotes or
T14988 35360-35366 VB denotes kinase
T14989 35367-35376 JJ denotes deficient
T14990 35377-35381 NNP denotes PKCα
T14991 35382-35392 NNS denotes constructs
T14992 35393-35396 VBD denotes did
T14993 35397-35400 RB denotes not
T14994 35401-35407 VB denotes affect
T14995 35408-35412 DT denotes this
T14996 35413-35428 NN denotes phosphorylation
T14997 35429-35434 NN denotes event
T14998 35434-35435 . denotes .
T14999 35436-35444 RB denotes Moreover
T15000 35444-35445 , denotes ,
T15001 35446-35452 JJ denotes mutant
T15002 35453-35463 NNS denotes constructs
T15003 35464-35474 VBG denotes containing
T15004 35475-35476 DT denotes a
T15005 35477-35482 NN denotes point
T15006 35483-35491 NN denotes mutation
T15007 35492-35494 IN denotes at
T15008 35495-35501 NNP denotes Ser536
T15009 35502-35504 IN denotes of
T15010 35505-35510 NNP denotes NF-κB
T15011 35511-35514 CD denotes p65
T15012 35515-35518 VBD denotes did
T15013 35519-35522 RB denotes not
T15014 35523-35529 VB denotes reduce
T15015 35530-35535 JJ denotes NF-κB
T15016 35536-35544 NN denotes activity
T15017 35545-35547 IN denotes in
T15018 35548-35553 NNS denotes cells
T15019 35554-35561 VBG denotes lacking
T15020 35562-35572 JJ denotes endogenous
T15021 35573-35578 NNP denotes NF-κB
T15022 35579-35582 NNS denotes p65
T15023 35582-35583 , denotes ,
T15024 35584-35589 IN denotes while
T15025 35590-35593 DT denotes the
T15026 35594-35599 NNP denotes NF-κB
T15027 35600-35603 CD denotes p65
T15028 35604-35610 NNP denotes 276S/A
T15029 35611-35620 VB denotes construct
T15030 35621-35624 VBD denotes did
T15031 35625-35631 VB denotes reduce
T15032 35632-35637 NN denotes NF-κB
T15033 35638-35646 NN denotes activity
T15034 35647-35649 IN denotes in
T15035 35650-35655 DT denotes these
T15036 35656-35661 NNS denotes cells
T15037 35661-35662 . denotes .
T15038 35663-35672 RB denotes Therefore
T15039 35672-35673 , denotes ,
T15040 35674-35682 JJ denotes specific
T15041 35683-35701 JJ denotes post-translational
T15042 35702-35714 NN denotes modification
T15043 35715-35717 IN denotes of
T15044 35718-35723 NNP denotes NF-κB
T15045 35724-35727 CD denotes p65
T15046 35728-35730 VBZ denotes is
T15047 35731-35737 JJ denotes likely
T15048 35738-35740 TO denotes to
T15049 35741-35749 VB denotes regulate
T15050 35750-35765 JJ denotes transcriptional
T15051 35766-35776 NN denotes activation
T15052 35777-35779 IN denotes in
T15053 35780-35788 NN denotes response
T15054 35789-35791 TO denotes to
T15055 35792-35800 JJ denotes specific
T15056 35801-35808 NNS denotes stimuli
T15057 35809-35810 NNP denotes [
T15058 35810-35812 CD denotes 15
T15059 35812-35813 NNP denotes ]
T15060 35813-35814 , denotes ,
T15061 35815-35816 NNP denotes [
T15062 35816-35818 CD denotes 43
T15063 35818-35819 NNP denotes ]
T15064 35819-35820 . denotes .
T15065 35821-35823 PRP denotes It
T15066 35824-35826 VBZ denotes is
T15067 35827-35834 JJ denotes notable
T15068 35835-35839 IN denotes that
T15069 35840-35845 NNP denotes M-CSF
T15070 35846-35855 VBD denotes activated
T15071 35856-35861 NNP denotes NF-κB
T15072 35862-35865 CD denotes p65
T15073 35866-35872 NNP denotes Ser276
T15074 35873-35888 NN denotes phosphorylation
T15075 35889-35892 CC denotes and
T15076 35893-35908 JJ denotes transcriptional
T15077 35909-35919 NN denotes activation
T15078 35920-35922 IN denotes in
T15079 35923-35924 DT denotes a
T15080 35925-35939 JJ denotes PKCα-dependent
T15081 35940-35946 NN denotes manner
T15082 35946-35947 . denotes .
T15083 35948-35952 NNP denotes PKCα
T15084 35953-35955 VBZ denotes is
T15085 35956-35957 DT denotes a
T15086 35958-35964 NN denotes member
T15087 35965-35967 IN denotes of
T15088 35968-35971 DT denotes the
T15089 35972-35984 JJ denotes conventional
T15090 35985-35988 NNP denotes PKC
T15091 35989-35995 NN denotes family
T15092 35996-35998 IN denotes of
T15093 35999-36006 NN denotes protein
T15094 36007-36014 NNS denotes kinases
T15095 36015-36019 WDT denotes that
T15096 36020-36023 VBP denotes are
T15097 36024-36032 JJ denotes critical
T15098 36033-36036 IN denotes for
T15099 36037-36041 NN denotes cell
T15100 36042-36048 NN denotes growth
T15101 36048-36049 , denotes ,
T15102 36050-36065 NN denotes differentiation
T15103 36066-36069 CC denotes and
T15104 36070-36074 NN denotes cell
T15105 36075-36080 NN denotes death
T15106 36080-36081 . denotes .
T15107 36082-36086 NNP denotes PKCα
T15108 36087-36096 RB denotes primarily
T15109 36097-36106 VBZ denotes modulates
T15110 36107-36121 JJ denotes anti-apoptotic
T15111 36122-36125 CC denotes and
T15112 36126-36139 NN denotes proliferation
T15113 36140-36147 NNS denotes signals
T15114 36148-36157 VBG denotes following
T15115 36158-36166 JJ denotes cytokine
T15116 36167-36176 NN denotes treatment
T15117 36177-36179 IN denotes in
T15118 36180-36187 JJ denotes various
T15119 36188-36192 NN denotes cell
T15120 36193-36198 NNS denotes types
T15121 36199-36200 NNP denotes [
T15122 36200-36202 CD denotes 42
T15123 36202-36203 NNP denotes ]
T15124 36203-36204 . denotes .
T15125 36205-36215 NN denotes Expression
T15126 36216-36218 IN denotes of
T15127 36219-36223 NNP denotes PKCα
T15128 36224-36234 VBZ denotes attenuates
T15129 36235-36244 NN denotes apoptosis
T15130 36245-36247 IN denotes in
T15131 36248-36252 JJ denotes many
T15132 36253-36262 JJ denotes different
T15133 36263-36267 NN denotes cell
T15134 36268-36273 NNS denotes types
T15135 36273-36274 , denotes ,
T15136 36275-36280 IN denotes while
T15137 36281-36285 NNP denotes PKCα
T15138 36286-36296 NN denotes inhibition
T15139 36297-36306 RB denotes generally
T15140 36307-36318 VBZ denotes potentiates
T15141 36319-36328 NN denotes apoptosis
T15142 36329-36330 NNP denotes [
T15143 36330-36332 CD denotes 42
T15144 36332-36333 NNP denotes ]
T15145 36333-36334 . denotes .
T15146 36335-36337 IN denotes In
T15147 36338-36341 PRP$ denotes our
T15148 36342-36349 NNS denotes studies
T15149 36350-36355 VBG denotes using
T15150 36356-36368 JJ denotes conventional
T15151 36369-36372 NNP denotes PKC
T15152 36373-36383 NNS denotes inhibitors
T15153 36384-36387 CC denotes and
T15154 36388-36396 JJ denotes dominant
T15155 36397-36405 JJ denotes negative
T15156 36406-36410 NNP denotes PKCα
T15157 36411-36421 NNS denotes constructs
T15158 36421-36422 , denotes ,
T15159 36423-36427 NNP denotes PKCα
T15160 36428-36431 VBD denotes was
T15161 36432-36440 JJ denotes critical
T15162 36441-36443 IN denotes in
T15163 36444-36457 JJ denotes M-CSF-induced
T15164 36458-36468 NN denotes macrophage
T15165 36469-36477 NN denotes survival
T15166 36478-36480 IN denotes by
T15167 36481-36489 VBG denotes inducing
T15168 36490-36496 NNP denotes Ser276
T15169 36497-36512 NN denotes phosphorylation
T15170 36513-36515 IN denotes of
T15171 36516-36521 NNP denotes NF-κB
T15172 36522-36525 CD denotes p65
T15173 36526-36528 IN denotes in
T15174 36529-36533 DT denotes both
T15175 36534-36541 JJ denotes primary
T15176 36542-36547 JJ denotes human
T15177 36548-36552 NNS denotes MDMs
T15178 36553-36556 CC denotes and
T15179 36557-36560 NNP denotes Raw
T15180 36561-36566 CD denotes 264.7
T15181 36567-36572 NNS denotes cells
T15182 36572-36573 . denotes .
T15183 36574-36576 PRP denotes We
T15184 36577-36581 RB denotes also
T15185 36582-36594 VBD denotes demonstrated
T15186 36595-36598 NNP denotes PKC
T15187 36599-36609 NN denotes inhibition
T15188 36610-36623 VBD denotes downregulated
T15189 36624-36639 NNP denotes NF-κB-induction
T15190 36640-36642 IN denotes of
T15191 36643-36646 DT denotes the
T15192 36647-36661 JJ denotes anti-apoptotic
T15193 36662-36666 NN denotes gene
T15194 36667-36673 NN denotes BCL-xL
T15195 36673-36674 . denotes .
T15196 36675-36685 JJ denotes Consistent
T15197 36686-36690 IN denotes with
T15198 36691-36694 PRP$ denotes our
T15199 36695-36702 NN denotes finding
T15200 36702-36703 , denotes ,
T15201 36704-36710 NNS denotes others
T15202 36711-36719 VBD denotes reported
T15203 36720-36725 JJ denotes NF-κB
T15204 36726-36735 VBN denotes regulated
T15205 36736-36739 DT denotes the
T15206 36740-36744 NN denotes gene
T15207 36745-36755 NN denotes expression
T15208 36756-36758 IN denotes of
T15209 36759-36763 JJ denotes many
T15210 36764-36778 JJ denotes anti-apoptotic
T15211 36779-36787 NNS denotes proteins
T15212 36788-36797 VBG denotes including
T15213 36798-36803 JJ denotes other
T15214 36804-36809 NNP denotes BCL-2
T15215 36810-36816 NN denotes family
T15216 36817-36824 NNS denotes members
T15217 36825-36828 CC denotes and
T15218 36829-36838 NN denotes inhibitor
T15219 36839-36841 IN denotes of
T15220 36842-36851 NN denotes apoptosis
T15221 36852-36853 -LRB- denotes (
T15222 36853-36856 NNP denotes IAP
T15223 36856-36857 -RRB- denotes )
T15224 36858-36866 NNS denotes proteins
T15225 36867-36872 WDT denotes which
T15226 36873-36876 VBP denotes are
T15227 36877-36887 NNS denotes regulators
T15228 36888-36890 IN denotes of
T15229 36891-36900 NN denotes apoptosis
T15230 36901-36904 CC denotes and
T15231 36905-36913 NN denotes function
T15232 36914-36922 RB denotes upstream
T15233 36923-36925 IN denotes of
T15234 36926-36934 NNS denotes caspases
T15235 36935-36936 NNP denotes [
T15236 36936-36938 CD denotes 44
T15237 36938-36939 NNP denotes ]
T15238 36939-36940 . denotes .
T15239 36941-36943 PRP denotes It
T15240 36944-36947 VBZ denotes has
T15241 36948-36952 VBN denotes been
T15242 36953-36958 VBN denotes shown
T15243 36959-36963 IN denotes that
T15244 36964-36979 NN denotes phosphorylation
T15245 36980-36982 IN denotes of
T15246 36983-36986 NNS denotes p65
T15247 36987-36989 IN denotes at
T15248 36990-36996 NNP denotes Ser276
T15249 36997-37005 VBZ denotes prevents
T15250 37006-37009 PRP$ denotes its
T15251 37010-37021 NN denotes degradation
T15252 37022-37024 IN denotes by
T15253 37025-37043 JJ denotes ubiquitin-mediated
T15254 37044-37055 NN denotes proteolysis
T15255 37056-37059 CC denotes and
T15256 37060-37068 VBZ denotes promotes
T15257 37069-37073 NN denotes cell
T15258 37074-37082 NN denotes survival
T15259 37083-37085 IN denotes in
T15260 37086-37090 NNP denotes HeLa
T15261 37091-37096 NNS denotes cells
T15262 37097-37098 NNP denotes [
T15263 37098-37100 CD denotes 24
T15264 37100-37101 NNP denotes ]
T15265 37101-37102 . denotes .
T15266 37103-37105 IN denotes In
T15267 37106-37114 NN denotes addition
T15268 37115-37117 TO denotes to
T15269 37118-37124 JJ denotes BCL-xL
T15270 37125-37135 NN denotes expression
T15271 37136-37138 IN denotes as
T15272 37139-37147 VBN denotes examined
T15273 37148-37150 IN denotes in
T15274 37151-37155 DT denotes this
T15275 37156-37161 NN denotes study
T15276 37161-37162 , denotes ,
T15277 37163-37165 PRP denotes we
T15278 37166-37169 VBP denotes are
T15279 37170-37180 VBG denotes evaluating
T15280 37181-37184 DT denotes the
T15281 37185-37196 NN denotes possibility
T15282 37197-37201 IN denotes that
T15283 37202-37216 JJ denotes PKCα-regulated
T15284 37217-37222 NN denotes NF-κB
T15285 37223-37231 NN denotes activity
T15286 37232-37243 VBZ denotes upregulates
T15287 37244-37254 JJ denotes additional
T15288 37255-37269 JJ denotes anti-apoptotic
T15289 37270-37275 NNS denotes genes
T15290 37276-37278 IN denotes in
T15291 37279-37281 DT denotes an
T15292 37282-37297 JJ denotes M-CSF-dependent
T15293 37298-37305 NN denotes fashion
T15294 37306-37308 TO denotes to
T15295 37309-37317 VB denotes modulate
T15296 37318-37322 NN denotes cell
T15297 37323-37331 NN denotes survival
T15298 37331-37332 . denotes .
T15299 37333-37336 DT denotes The
T15300 37337-37340 NN denotes use
T15301 37341-37343 IN denotes of
T15302 37344-37354 NNS denotes inhibitors
T15303 37355-37358 CC denotes and
T15304 37359-37364 NN denotes siRNA
T15305 37365-37367 IN denotes in
T15306 37368-37371 PRP$ denotes our
T15307 37372-37377 NN denotes study
T15308 37378-37381 VBZ denotes has
T15309 37382-37385 RB denotes not
T15310 37386-37391 VBN denotes ruled
T15311 37392-37395 RP denotes out
T15312 37396-37400 IN denotes that
T15313 37401-37406 JJ denotes other
T15314 37407-37419 JJ denotes conventional
T15315 37420-37424 NNS denotes PKCs
T15316 37425-37430 MD denotes might
T15317 37431-37435 RB denotes also
T15318 37436-37438 VB denotes be
T15319 37439-37448 JJ denotes important
T15320 37449-37451 IN denotes in
T15321 37452-37465 JJ denotes M-CSF-induced
T15322 37466-37471 NN denotes NF-κB
T15323 37472-37482 NN denotes activation
T15324 37483-37486 CC denotes and
T15325 37487-37497 NN denotes macrophage
T15326 37498-37506 NN denotes survival
T15327 37506-37507 . denotes .
T15328 37508-37510 IN denotes In
T15329 37511-37519 NN denotes contrast
T15330 37520-37522 TO denotes to
T15331 37523-37526 DT denotes the
T15332 37527-37534 NNS denotes actions
T15333 37535-37537 IN denotes of
T15334 37538-37542 NNP denotes PKCα
T15335 37543-37553 NN denotes activation
T15336 37554-37556 IN denotes of
T15337 37557-37562 JJ denotes other
T15338 37563-37575 JJ denotes conventional
T15339 37576-37579 NNP denotes PKC
T15340 37580-37588 NNS denotes isoforms
T15341 37588-37589 , denotes ,
T15342 37590-37594 IN denotes like
T15343 37595-37600 NNP denotes PKCβI
T15344 37600-37601 NNP denotes /
T15345 37601-37604 NNP denotes βII
T15346 37604-37605 , denotes ,
T15347 37606-37612 VBP denotes appear
T15348 37613-37622 JJ denotes necessary
T15349 37623-37626 IN denotes for
T15350 37627-37637 NN denotes macrophage
T15351 37638-37647 NN denotes apoptosis
T15352 37647-37648 . denotes .
T15353 37649-37652 DT denotes The
T15354 37653-37658 NNP denotes PKCβI
T15355 37658-37659 NNP denotes /
T15356 37659-37662 NNP denotes βII
T15357 37663-37671 NNS denotes isoforms
T15358 37672-37675 VBP denotes are
T15359 37676-37685 VBN denotes expressed
T15360 37686-37688 IN denotes in
T15361 37689-37698 JJ denotes apoptotic
T15362 37699-37703 NNP denotes U937
T15363 37704-37718 JJ denotes myelomonocytic
T15364 37719-37724 NNS denotes cells
T15365 37725-37726 NNP denotes [
T15366 37726-37728 CD denotes 45
T15367 37728-37729 NNP denotes ]
T15368 37729-37730 . denotes .
T15369 37731-37734 DT denotes The
T15370 37735-37740 JJ denotes human
T15371 37741-37748 JJ denotes myeloid
T15372 37749-37753 NN denotes cell
T15373 37754-37758 NN denotes line
T15374 37759-37765 NN denotes HL-525
T15375 37766-37771 WDT denotes which
T15376 37772-37774 VBZ denotes is
T15377 37775-37781 JJ denotes devoid
T15378 37782-37784 IN denotes of
T15379 37785-37795 JJ denotes endogenous
T15380 37796-37802 NNP denotes PKCβII
T15381 37803-37805 VBZ denotes is
T15382 37806-37815 JJ denotes resistant
T15383 37816-37818 TO denotes to
T15384 37819-37831 JJ denotes TNFα-induced
T15385 37832-37841 NN denotes apoptosis
T15386 37842-37843 NNP denotes [
T15387 37843-37845 CD denotes 46
T15388 37845-37846 NNP denotes ]
T15389 37846-37847 . denotes .
T15390 37848-37861 NN denotes Re-expression
T15391 37862-37864 IN denotes of
T15392 37865-37871 NNP denotes PKCβII
T15393 37872-37874 IN denotes in
T15394 37875-37881 NNP denotes HL-525
T15395 37882-37887 NNS denotes cells
T15396 37888-37896 VBZ denotes restores
T15397 37897-37902 PRP$ denotes their
T15398 37903-37917 NN denotes susceptibility
T15399 37918-37920 TO denotes to
T15400 37921-37933 JJ denotes TNFα-induced
T15401 37934-37943 NN denotes apoptosis
T15402 37944-37952 VBG denotes implying
T15403 37953-37957 IN denotes that
T15404 37958-37964 NNP denotes PKCβII
T15405 37965-37967 VBZ denotes is
T15406 37968-37981 JJ denotes pro-apoptotic
T15407 37982-37985 CC denotes and
T15408 37986-37989 MD denotes may
T15409 37990-37992 VB denotes be
T15410 37993-38001 VBN denotes required
T15411 38002-38004 TO denotes to
T15412 38005-38011 VB denotes induce
T15413 38012-38022 NN denotes macrophage
T15414 38023-38032 NN denotes apoptosis
T15415 38032-38033 . denotes .
T15416 38034-38040 JJ denotes Recent
T15417 38041-38048 NNS denotes studies
T15418 38049-38061 VBD denotes demonstrated
T15419 38062-38066 IN denotes that
T15420 38067-38072 JJ denotes NF-κB
T15421 38073-38088 JJ denotes transcriptional
T15422 38089-38097 NN denotes activity
T15423 38098-38101 MD denotes can
T15424 38102-38104 VB denotes be
T15425 38105-38114 VBN denotes regulated
T15426 38115-38117 IN denotes by
T15427 38118-38133 NN denotes phosphorylation
T15428 38134-38136 IN denotes of
T15429 38137-38140 DT denotes the
T15430 38141-38146 NNP denotes NF-κB
T15431 38147-38150 CD denotes p65
T15432 38151-38158 NN denotes subunit
T15433 38159-38161 IN denotes in
T15434 38162-38165 DT denotes the
T15435 38166-38173 NN denotes absence
T15436 38174-38176 IN denotes of
T15437 38177-38181 NNP denotes IκBα
T15438 38182-38193 NN denotes degradation
T15439 38194-38195 NNP denotes [
T15440 38195-38197 CD denotes 47
T15441 38197-38198 NNP denotes ]
T15442 38198-38199 . denotes .
T15443 38200-38202 IN denotes By
T15444 38203-38207 DT denotes this
T15445 38208-38215 NN denotes pathway
T15446 38215-38216 , denotes ,
T15447 38217-38232 NN denotes phosphorylation
T15448 38233-38235 IN denotes of
T15449 38236-38241 JJ denotes NF-κB
T15450 38242-38251 VBZ denotes regulates
T15451 38252-38255 PRP$ denotes its
T15452 38256-38267 NN denotes interaction
T15453 38268-38272 IN denotes with
T15454 38273-38278 JJ denotes other
T15455 38279-38289 NNS denotes components
T15456 38290-38292 IN denotes of
T15457 38293-38296 DT denotes the
T15458 38297-38302 JJ denotes basal
T15459 38303-38318 JJ denotes transcriptional
T15460 38319-38328 NN denotes machinery
T15461 38329-38336 IN denotes without
T15462 38337-38346 VBG denotes affecting
T15463 38347-38350 PRP$ denotes its
T15464 38351-38361 NN denotes capability
T15465 38362-38364 TO denotes to
T15466 38365-38369 NN denotes bind
T15467 38370-38373 NNP denotes DNA
T15468 38374-38375 NNP denotes [
T15469 38375-38377 CD denotes 21
T15470 38377-38378 NNP denotes ]
T15471 38378-38379 . denotes .
T15472 38380-38385 IN denotes Since
T15473 38386-38391 NNP denotes NF-κB
T15474 38392-38395 MD denotes can
T15475 38396-38400 NN denotes bind
T15476 38401-38404 DT denotes the
T15477 38405-38414 NNS denotes promoters
T15478 38415-38417 IN denotes of
T15479 38418-38426 JJ denotes numerous
T15480 38427-38431 NN denotes gene
T15481 38432-38439 NNS denotes targets
T15482 38439-38440 , denotes ,
T15483 38441-38459 JJ denotes post-translational
T15484 38460-38472 NN denotes modification
T15485 38473-38475 IN denotes of
T15486 38476-38481 NNP denotes NF-κB
T15487 38482-38485 NNS denotes p65
T15488 38486-38489 MD denotes may
T15489 38490-38496 VB denotes affect
T15490 38497-38500 PRP$ denotes its
T15491 38501-38512 NN denotes interaction
T15492 38513-38517 IN denotes with
T15493 38518-38523 JJ denotes other
T15494 38524-38532 NNS denotes proteins
T15495 38532-38533 , denotes ,
T15496 38534-38542 VBG denotes refining
T15497 38543-38546 DT denotes the
T15498 38547-38557 NN denotes expression
T15499 38558-38560 IN denotes of
T15500 38561-38565 NN denotes gene
T15501 38566-38573 NNS denotes targets
T15502 38573-38574 . denotes .
T15503 38575-38577 PRP denotes We
T15504 38578-38581 VBD denotes did
T15505 38582-38585 RB denotes not
T15506 38586-38590 VB denotes find
T15507 38591-38595 DT denotes that
T15508 38596-38599 NNP denotes PKC
T15509 38600-38603 VBD denotes was
T15510 38604-38612 VBN denotes involved
T15511 38613-38615 IN denotes in
T15512 38616-38620 NNP denotes IκBα
T15513 38621-38632 NN denotes degradation
T15514 38633-38635 CC denotes or
T15515 38636-38641 NN denotes NF-κB
T15516 38642-38645 NNS denotes p65
T15517 38646-38653 JJ denotes nuclear
T15518 38654-38667 NN denotes translocation
T15519 38668-38675 VBN denotes induced
T15520 38676-38678 IN denotes by
T15521 38679-38684 NNP denotes M-CSF
T15522 38684-38685 , denotes ,
T15523 38686-38689 CC denotes but
T15524 38690-38694 DT denotes that
T15525 38695-38708 JJ denotes M-CSF-induced
T15526 38709-38724 NN denotes phosphorylation
T15527 38725-38727 IN denotes of
T15528 38728-38733 NNP denotes NF-κB
T15529 38734-38737 NNS denotes p65
T15530 38738-38740 IN denotes at
T15531 38741-38747 NNP denotes Ser276
T15532 38748-38751 VBD denotes was
T15533 38752-38764 RB denotes dramatically
T15534 38765-38772 VBN denotes reduced
T15535 38773-38775 IN denotes by
T15536 38776-38779 DT denotes the
T15537 38780-38792 JJ denotes conventional
T15538 38793-38796 NNP denotes PKC
T15539 38797-38806 NN denotes inhibitor
T15540 38807-38810 CC denotes and
T15541 38811-38817 NN denotes kinase
T15542 38818-38827 NN denotes deficient
T15543 38828-38832 NNP denotes PKCα
T15544 38833-38836 CC denotes and
T15545 38837-38839 IN denotes by
T15546 38840-38845 NNP denotes siRNA
T15547 38846-38853 IN denotes towards
T15548 38854-38858 NNP denotes PKCα
T15549 38858-38859 . denotes .
T15550 38860-38867 JJ denotes Current
T15551 38868-38875 NNS denotes studies
T15552 38876-38879 VBP denotes are
T15553 38880-38888 JJ denotes underway
T15554 38889-38891 TO denotes to
T15555 38892-38901 VB denotes delineate
T15556 38902-38905 DT denotes the
T15557 38906-38913 NN denotes protein
T15558 38914-38923 NNS denotes complexes
T15559 38924-38930 VBN denotes formed
T15560 38931-38933 IN denotes by
T15561 38934-38943 VBN denotes activated
T15562 38944-38949 NNP denotes NF-κB
T15563 38950-38953 CC denotes and
T15564 38954-38959 JJ denotes other
T15565 38960-38975 JJ denotes transcriptional
T15566 38976-38989 NNS denotes co-activators
T15567 38990-38992 IN denotes in
T15568 38993-39001 NN denotes response
T15569 39002-39004 TO denotes to
T15570 39005-39010 NNP denotes M-CSF
T15571 39011-39014 CC denotes and
T15572 39015-39018 NNP denotes PKC
T15573 39019-39029 NN denotes activation
T15574 39029-39030 . denotes .
T15575 39031-39039 RB denotes Recently
T15576 39040-39047 NNS denotes studies
T15577 39048-39057 VBD denotes indicated
T15578 39058-39062 IN denotes that
T15579 39063-39066 DT denotes the
T15580 39067-39080 JJ denotes non-canonical
T15581 39081-39088 NN denotes pathway
T15582 39089-39092 VBD denotes was
T15583 39093-39102 VBN denotes activated
T15584 39103-39109 IN denotes during
T15585 39110-39115 JJ denotes human
T15586 39116-39135 NN denotes monocyte-macrophage
T15587 39136-39151 NN denotes differentiation
T15588 39152-39153 NNP denotes [
T15589 39153-39155 CD denotes 48
T15590 39155-39156 NNP denotes ]
T15591 39156-39157 . denotes .
T15592 39158-39164 IN denotes During
T15593 39165-39169 DT denotes this
T15594 39170-39177 NN denotes process
T15595 39177-39178 , denotes ,
T15596 39179-39189 NN denotes expression
T15597 39190-39192 IN denotes of
T15598 39193-39197 NNP denotes IKKα
T15599 39198-39203 IN denotes among
T15600 39204-39209 JJ denotes other
T15601 39210-39218 NNS denotes proteins
T15602 39218-39219 , denotes ,
T15603 39220-39222 VBZ denotes is
T15604 39223-39231 JJ denotes elevated
T15605 39232-39239 VBG denotes leading
T15606 39240-39242 TO denotes to
T15607 39243-39252 VBN denotes increased
T15608 39253-39256 CD denotes p52
T15609 39257-39262 NNP denotes NF-κB
T15610 39263-39264 -LRB- denotes (
T15611 39264-39268 NNP denotes relB
T15612 39268-39269 -RRB- denotes )
T15613 39270-39280 NN denotes expression
T15614 39281-39288 IN denotes through
T15615 39289-39296 JJ denotes partial
T15616 39297-39308 JJ denotes proteolytic
T15617 39309-39320 NN denotes degradation
T15618 39321-39323 IN denotes of
T15619 39324-39327 DT denotes the
T15620 39328-39332 CD denotes p100
T15621 39333-39338 NN denotes NF-κB
T15622 39339-39346 NN denotes protein
T15623 39346-39347 . denotes .
T15624 39348-39352 RB denotes Thus
T15625 39352-39353 , denotes ,
T15626 39354-39356 PRP denotes it
T15627 39357-39359 VBZ denotes is
T15628 39360-39368 JJ denotes possible
T15629 39369-39373 IN denotes that
T15630 39374-39379 NNP denotes M-CSF
T15631 39380-39389 VBZ denotes regulates
T15632 39390-39398 NN denotes monocyte
T15633 39399-39407 NN denotes survival
T15634 39408-39415 IN denotes through
T15635 39416-39420 DT denotes both
T15636 39421-39424 DT denotes the
T15637 39425-39434 JJ denotes canonical
T15638 39435-39438 CC denotes and
T15639 39439-39452 JJ denotes non-canonical
T15640 39453-39458 NN denotes NF-κB
T15641 39459-39467 NNS denotes pathways
T15642 39467-39468 , denotes ,
T15643 39469-39474 WDT denotes which
T15644 39475-39479 MD denotes will
T15645 39480-39482 VB denotes be
T15646 39483-39484 DT denotes a
T15647 39485-39490 NN denotes focus
T15648 39491-39493 IN denotes in
T15649 39494-39500 JJ denotes future
T15650 39501-39509 NN denotes research
T15651 39509-39510 . denotes .
T15652 39511-39513 PRP denotes We
T15653 39514-39524 RB denotes previously
T15654 39525-39531 VBD denotes showed
T15655 39532-39536 IN denotes that
T15656 39537-39542 NNP denotes M-CSF
T15657 39543-39550 VBD denotes reduced
T15658 39551-39560 JJ denotes caspase-3
T15659 39561-39564 CC denotes and
T15660 39565-39567 JJ denotes -9
T15661 39568-39576 NN denotes activity
T15662 39577-39580 CC denotes and
T15663 39581-39590 VBD denotes prevented
T15664 39591-39599 NN denotes monocyte
T15665 39600-39609 NN denotes apoptosis
T15666 39610-39611 NNP denotes [
T15667 39611-39612 CD denotes 3
T15668 39612-39613 NNP denotes ]
T15669 39613-39614 . denotes .
T15670 39615-39619 DT denotes This
T15671 39620-39625 NN denotes study
T15672 39626-39633 VBZ denotes reveals
T15673 39634-39638 IN denotes that
T15674 39639-39648 NN denotes treatment
T15675 39649-39651 IN denotes of
T15676 39652-39661 NNS denotes monocytes
T15677 39662-39666 IN denotes with
T15678 39667-39679 JJ denotes conventional
T15679 39680-39683 NNP denotes PKC
T15680 39684-39694 NNS denotes inhibitors
T15681 39694-39695 , denotes ,
T15682 39696-39698 CC denotes or
T15683 39699-39713 NN denotes overexpression
T15684 39714-39716 IN denotes of
T15685 39717-39733 JJ denotes kinase-deficient
T15686 39734-39738 NNP denotes PKCα
T15687 39739-39746 VBD denotes blocked
T15688 39747-39760 JJ denotes M-CSF-induced
T15689 39761-39765 NN denotes cell
T15690 39766-39774 NN denotes survival
T15691 39774-39775 . denotes .
T15692 39776-39779 PRP$ denotes Our
T15693 39780-39784 NNS denotes data
T15694 39785-39793 VBD denotes revealed
T15695 39794-39798 IN denotes that
T15696 39799-39811 JJ denotes conventional
T15697 39812-39816 NNS denotes PKCs
T15698 39817-39820 VBP denotes are
T15699 39821-39829 RB denotes upstream
T15700 39830-39832 IN denotes of
T15701 39833-39838 JJ denotes NF-κB
T15702 39839-39849 NN denotes activation
T15703 39850-39852 IN denotes in
T15704 39853-39861 NN denotes response
T15705 39862-39864 TO denotes to
T15706 39865-39870 NNP denotes M-CSF
T15707 39871-39874 CC denotes and
T15708 39875-39882 VB denotes mediate
T15709 39883-39901 JJ denotes post-translational
T15710 39902-39912 NN denotes activation
T15711 39913-39915 IN denotes of
T15712 39916-39921 NNP denotes NF-κB
T15713 39922-39925 CD denotes p65
T15714 39926-39928 IN denotes by
T15715 39929-39944 VBG denotes phosphorylating
T15716 39945-39951 NNP denotes Ser276
T15717 39952-39954 TO denotes to
T15718 39955-39963 VB denotes regulate
T15719 39964-39968 NN denotes gene
T15720 39969-39982 NN denotes transcription
T15721 39983-39986 CC denotes and
T15722 39987-39991 NN denotes cell
T15723 39992-40000 NN denotes survival
T15724 40000-40001 . denotes .
T15725 40002-40006 RB denotes Thus
T15726 40006-40007 , denotes ,
T15727 40008-40011 PRP$ denotes our
T15728 40012-40023 NN denotes observation
T15729 40024-40027 MD denotes may
T15730 40028-40035 VB denotes provide
T15731 40036-40043 NN denotes insight
T15732 40044-40046 IN denotes in
T15733 40047-40056 JJ denotes potential
T15734 40057-40068 JJ denotes therapeutic
T15735 40069-40076 NNS denotes targets
T15736 40077-40080 IN denotes for
T15737 40081-40093 JJ denotes inflammatory
T15738 40094-40102 NNS denotes diseases
T15739 40102-40103 . denotes .
T18733 40128-40137 NNPS denotes Materials
T18734 40138-40149 NNP denotes Recombinant
T18735 40150-40155 JJ denotes human
T18736 40156-40161 NNP denotes M-CSF
T18737 40162-40165 VBD denotes was
T18738 40166-40175 VBN denotes purchased
T18739 40176-40180 IN denotes from
T18740 40181-40184 NNP denotes R&D
T18741 40185-40192 NNP denotes Systems
T18742 40193-40194 -LRB- denotes (
T18743 40194-40205 NNP denotes Minneapolis
T18744 40205-40206 , denotes ,
T18745 40207-40209 NNP denotes MN
T18746 40209-40210 -RRB- denotes )
T18747 40210-40211 . denotes .
T18748 40212-40222 NN denotes Ro-31-8220
T18749 40222-40223 , denotes ,
T18750 40224-40231 NNP denotes Gö-6976
T18751 40231-40232 , denotes ,
T18752 40233-40241 NNP denotes Rotterin
T18753 40241-40242 , denotes ,
T18754 40243-40246 CC denotes and
T18755 40247-40255 NNP denotes BAPTA/AM
T18756 40256-40260 VBD denotes were
T18757 40261-40269 VBN denotes obtained
T18758 40270-40274 IN denotes from
T18759 40275-40285 NNP denotes Calbiochem
T18760 40286-40287 -LRB- denotes (
T18761 40287-40290 NNP denotes San
T18762 40291-40296 NNP denotes Diego
T18763 40296-40297 , denotes ,
T18764 40298-40300 NNP denotes CA
T18765 40300-40301 -RRB- denotes )
T18766 40301-40302 . denotes .
T18767 40303-40306 NNP denotes Low
T18768 40307-40316 NN denotes endotoxin
T18769 40317-40321 NNP denotes RPMI
T18770 40322-40325 CC denotes and
T18771 40326-40332 NNP denotes X-vivo
T18772 40333-40338 VBP denotes serum
T18773 40339-40343 JJ denotes free
T18774 40344-40350 NN denotes medium
T18775 40351-40355 VBD denotes were
T18776 40356-40364 VBN denotes obtained
T18777 40365-40369 IN denotes from
T18778 40370-40375 NNP denotes Lonza
T18779 40376-40388 NNP denotes Walkersville
T18780 40389-40392 NNP denotes Inc
T18781 40393-40394 -LRB- denotes (
T18782 40394-40406 NNP denotes Walkersville
T18783 40406-40407 , denotes ,
T18784 40408-40410 NNP denotes MD
T18785 40410-40411 -RRB- denotes )
T18786 40411-40412 . denotes .
T18787 40413-40418 JJ denotes Fetal
T18788 40419-40425 JJ denotes bovine
T18789 40426-40431 NN denotes serum
T18790 40432-40433 -LRB- denotes (
T18791 40433-40436 NNP denotes FBS
T18792 40436-40437 , denotes ,
T18793 40438-40447 VBD denotes certified
T18794 40448-40449 CD denotes <
T18795 40449-40453 CD denotes 0.06
T18796 40454-40463 NN denotes endotoxin
T18797 40464-40472 NN denotes units/ml
T18798 40473-40482 NN denotes endotoxin
T18799 40483-40489 NNS denotes levels
T18800 40489-40490 -RRB- denotes )
T18801 40491-40494 VBD denotes was
T18802 40495-40504 VBN denotes purchased
T18803 40505-40509 IN denotes from
T18804 40510-40517 NNP denotes Atlanta
T18805 40518-40528 NNP denotes Biological
T18806 40529-40530 -LRB- denotes (
T18807 40530-40543 NNP denotes Lawrenceville
T18808 40543-40544 , denotes ,
T18809 40545-40547 NNP denotes GA
T18810 40547-40548 -RRB- denotes )
T18811 40548-40549 . denotes .
T18812 40550-40553 DT denotes All
T18813 40554-40559 JJ denotes other
T18814 40560-40564 NN denotes cell
T18815 40565-40572 NN denotes culture
T18816 40573-40581 NNS denotes reagents
T18817 40582-40586 VBD denotes were
T18818 40587-40595 VBN denotes obtained
T18819 40596-40603 IN denotes through
T18820 40604-40614 NNP denotes Invitrogen
T18821 40615-40616 -LRB- denotes (
T18822 40616-40624 NNP denotes Carlsbad
T18823 40624-40625 , denotes ,
T18824 40626-40628 NNP denotes CA
T18825 40628-40629 -RRB- denotes )
T18826 40629-40630 . denotes .
T18827 40631-40635 NNP denotes PKCα
T18828 40636-40641 NNP denotes NF-κB
T18829 40642-40645 NNS denotes p65
T18830 40645-40646 , denotes ,
T18831 40647-40652 NNP denotes Lamin
T18832 40653-40654 NNP denotes B
T18833 40654-40655 , denotes ,
T18834 40656-40663 NN denotes β-actin
T18835 40663-40664 , denotes ,
T18836 40665-40668 CC denotes and
T18837 40669-40674 NNP denotes GAPDH
T18838 40675-40685 NNS denotes antibodies
T18839 40685-40686 , denotes ,
T18840 40687-40697 JJ denotes anti-mouse
T18841 40698-40701 CC denotes and
T18842 40702-40708 NN denotes rabbit
T18843 40709-40716 JJ denotes IgG-HRP
T18844 40717-40727 VBN denotes conjugated
T18845 40728-40738 NNS denotes antibodies
T18846 40738-40739 , denotes ,
T18847 40740-40743 CC denotes and
T18848 40744-40748 NNP denotes PKCα
T18849 40749-40754 NNP denotes siRNA
T18850 40755-40759 VBD denotes were
T18851 40760-40768 VBN denotes obtained
T18852 40769-40773 IN denotes from
T18853 40774-40779 NNP denotes Santa
T18854 40780-40784 NNP denotes Cruz
T18855 40785-40798 NNP denotes Biotechnology
T18856 40799-40800 -LRB- denotes (
T18857 40800-40805 NNP denotes Santa
T18858 40806-40810 NNP denotes Cruz
T18859 40810-40811 , denotes ,
T18860 40812-40814 NNP denotes CA
T18861 40814-40815 -RRB- denotes )
T18862 40815-40816 . denotes .
T18863 40817-40829 NNP denotes Phospho-NFκB
T18864 40830-40833 NNS denotes p65
T18865 40834-40835 -LRB- denotes (
T18866 40835-40841 CD denotes Ser276
T18867 40842-40844 CC denotes or
T18868 40845-40851 CD denotes Ser536
T18869 40851-40852 -RRB- denotes )
T18870 40853-40863 NNS denotes antibodies
T18871 40864-40868 VBD denotes were
T18872 40869-40878 VBN denotes purchased
T18873 40879-40883 IN denotes from
T18874 40884-40888 NNP denotes Cell
T18875 40889-40898 NNP denotes Signaling
T18876 40899-40909 NNP denotes Technology
T18877 40910-40911 -LRB- denotes (
T18878 40911-40918 NNP denotes Danvers
T18879 40918-40919 , denotes ,
T18880 40920-40922 NNP denotes MA
T18881 40922-40923 -RRB- denotes )
T18882 40923-40924 . denotes .
T18883 40925-40928 NNP denotes DNA
T18884 40929-40939 VBZ denotes constructs
T18885 40940-40949 NNP denotes pTAL-SEAP
T18886 40950-40953 CC denotes and
T18887 40954-40965 NNP denotes pNF-κB-SEAP
T18888 40966-40970 VBD denotes were
T18889 40971-40979 VBN denotes obtained
T18890 40980-40984 IN denotes from
T18891 40985-40993 NNP denotes Clontech
T18892 40994-40995 -LRB- denotes (
T18893 40995-41003 NNP denotes Mountian
T18894 41004-41008 NNP denotes View
T18895 41008-41009 , denotes ,
T18896 41010-41012 NNP denotes CA
T18897 41012-41013 -RRB- denotes )
T18898 41013-41014 . denotes .
T18899 41015-41025 NN denotes Expression
T18900 41026-41033 NNS denotes vectors
T18901 41034-41044 VBG denotes containing
T18902 41045-41052 NNP denotes CMV-p65
T18903 41053-41055 NNP denotes WT
T18904 41055-41056 , denotes ,
T18905 41057-41064 NNP denotes CMV-p65
T18906 41065-41071 NNP denotes 276S/A
T18907 41072-41075 CC denotes and
T18908 41076-41083 NNP denotes CMV-p65
T18909 41084-41090 NNP denotes 536S/A
T18910 41091-41095 VBD denotes were
T18911 41096-41102 VBN denotes cloned
T18912 41103-41105 IN denotes in
T18913 41106-41111 NNP denotes pBa5e
T18914 41112-41116 NNP denotes Puro
T18915 41117-41123 NN denotes vector
T18916 41124-41126 CC denotes or
T18917 41127-41138 NN denotes pFLAG-CMV-2
T18918 41139-41145 NN denotes vector
T18919 41146-41148 IN denotes as
T18920 41149-41158 VBN denotes described
T18921 41159-41169 RB denotes previously
T18922 41170-41171 NNP denotes [
T18923 41171-41173 CD denotes 49
T18924 41173-41174 NNP denotes ]
T18925 41174-41175 . denotes .
T18926 41176-41182 NNP denotes Kinase
T18927 41183-41192 NN denotes deficient
T18928 41193-41201 NNP denotes CMV-PKCα
T18929 41202-41203 -LRB- denotes (
T18930 41203-41210 NNP denotes KD-PKCα
T18931 41210-41211 -RRB- denotes )
T18932 41212-41214 CC denotes or
T18933 41215-41219 JJ denotes wild
T18934 41220-41224 NN denotes type
T18935 41225-41234 NN denotes pCMV-PKCα
T18936 41235-41236 -LRB- denotes (
T18937 41236-41243 NNP denotes WT-PKCα
T18938 41243-41244 -RRB- denotes )
T18939 41245-41249 VBD denotes were
T18940 41250-41258 JJ denotes generous
T18941 41259-41264 NNS denotes gifts
T18942 41265-41269 IN denotes from
T18943 41270-41279 NNP denotes Alexandra
T18944 41280-41282 NNP denotes C.
T18945 41283-41289 NNP denotes Newton
T18946 41290-41291 -LRB- denotes (
T18947 41291-41301 NNP denotes University
T18948 41302-41304 IN denotes of
T18949 41305-41315 NNP denotes California
T18950 41315-41316 , denotes ,
T18951 41317-41320 NNP denotes San
T18952 41321-41326 NNP denotes Diego
T18953 41326-41327 , denotes ,
T18954 41328-41330 NNP denotes CA
T18955 41330-41331 -RRB- denotes )
T18956 41331-41332 . denotes .
T18957 41333-41337 NNP denotes IκBα
T18958 41338-41339 -LRB- denotes (
T18959 41339-41345 NNP denotes NFKBIA
T18960 41345-41346 -RRB- denotes )
T18961 41347-41350 CC denotes and
T18962 41351-41356 NNP denotes GAPDH
T18963 41357-41364 NNS denotes primers
T18964 41365-41368 IN denotes for
T18965 41369-41378 JJ denotes real-time
T18966 41379-41385 NN denotes RT-PCR
T18967 41386-41390 VBD denotes were
T18968 41391-41399 VBN denotes obtained
T18969 41400-41404 IN denotes from
T18970 41405-41418 NNS denotes SABiosciences
T18971 41419-41420 -LRB- denotes (
T18972 41420-41429 NNP denotes Frederick
T18973 41429-41430 , denotes ,
T18974 41431-41433 NNP denotes MD
T18975 41433-41434 -RRB- denotes )
T18976 41434-41435 . denotes .
T18977 41436-41437 DT denotes A
T18978 41438-41442 NN denotes pool
T18979 41443-41445 IN denotes of
T18980 41446-41451 NN denotes mouse
T18981 41452-41454 CC denotes or
T18982 41455-41460 JJ denotes human
T18983 41461-41465 NNP denotes PKCα
T18984 41466-41474 JJ denotes specific
T18985 41475-41480 NNP denotes siRNA
T18986 41481-41485 VBD denotes were
T18987 41486-41495 VBN denotes purchased
T18988 41496-41500 IN denotes from
T18989 41501-41506 NNP denotes Santa
T18990 41507-41511 NNP denotes Cruz
T18991 41512-41525 NNP denotes Biotechnology
T18992 41526-41527 -LRB- denotes (
T18993 41527-41532 NNP denotes Santa
T18994 41533-41537 NNP denotes cruz
T18995 41537-41538 , denotes ,
T18996 41539-41541 NNP denotes CA
T18997 41541-41542 -RRB- denotes )
T18998 41542-41543 . denotes .
T19447 41545-41549 NNP denotes Cell
T19448 41550-41557 NNP denotes Culture
T19449 41558-41561 NNP denotes The
T19450 41562-41568 JJ denotes murine
T19451 41569-41579 NN denotes macrophage
T19452 41580-41584 NN denotes cell
T19453 41585-41589 NN denotes line
T19454 41590-41593 NNP denotes RAW
T19455 41594-41599 CD denotes 264.7
T19456 41600-41603 VBD denotes was
T19457 41604-41613 VBN denotes purchased
T19458 41614-41618 IN denotes from
T19459 41619-41623 NNP denotes ATCC
T19460 41624-41625 -LRB- denotes (
T19461 41625-41633 NNP denotes Manassas
T19462 41633-41634 , denotes ,
T19463 41635-41637 NNP denotes VA
T19464 41637-41638 -RRB- denotes )
T19465 41638-41639 . denotes .
T19466 41640-41643 NNP denotes RAW
T19467 41644-41649 CD denotes 264.7
T19468 41650-41655 NNS denotes cells
T19469 41656-41660 VBD denotes were
T19470 41661-41671 VBN denotes maintained
T19471 41672-41674 IN denotes in
T19472 41675-41679 NNP denotes RPMI
T19473 41680-41692 VBN denotes supplemented
T19474 41693-41697 IN denotes with
T19475 41698-41699 CD denotes 5
T19476 41699-41700 NN denotes %
T19477 41701-41704 NNP denotes FBS
T19478 41705-41708 CC denotes and
T19479 41709-41731 JJ denotes antibiotic-antimycotic
T19480 41732-41733 -LRB- denotes (
T19481 41733-41737 CD denotes 1000
T19482 41738-41742 NNP denotes U/ml
T19483 41743-41753 NN denotes penicillin
T19484 41753-41754 , denotes ,
T19485 41755-41759 CD denotes 1000
T19486 41760-41762 NN denotes µg
T19487 41762-41763 NN denotes /
T19488 41763-41765 NN denotes ml
T19489 41766-41778 NN denotes streptomycin
T19490 41779-41786 NN denotes sulfate
T19491 41786-41787 , denotes ,
T19492 41788-41791 CC denotes and
T19493 41792-41795 CD denotes 250
T19494 41796-41801 JJ denotes ng/ml
T19495 41802-41814 NN denotes amphotericin
T19496 41815-41816 NNP denotes B
T19497 41816-41817 -RRB- denotes )
T19498 41818-41820 IN denotes at
T19499 41821-41823 CD denotes 37
T19500 41823-41824 CD denotes °
T19501 41824-41826 NNP denotes C.
T19502 41827-41832 NNP denotes Mouse
T19503 41833-41842 JJ denotes embryonic
T19504 41843-41854 NNS denotes fibroblasts
T19505 41855-41856 -LRB- denotes (
T19506 41856-41860 NNS denotes MEFs
T19507 41860-41861 -RRB- denotes )
T19508 41862-41866 NN denotes cell
T19509 41867-41871 NN denotes line
T19510 41872-41879 VBG denotes lacking
T19511 41880-41888 JJ denotes specific
T19512 41889-41893 NNP denotes NFκB
T19513 41894-41903 VBG denotes signaling
T19514 41904-41912 NNS denotes subunits
T19515 41913-41918 NNP denotes NF-κB
T19516 41919-41922 NNS denotes p65
T19517 41923-41924 -LRB- denotes (
T19518 41924-41927 CD denotes p65
T19519 41927-41928 CD denotes −
T19520 41928-41929 NN denotes /
T19521 41929-41930 NN denotes −
T19522 41931-41935 NN denotes cell
T19523 41936-41940 NN denotes line
T19524 41940-41941 -RRB- denotes )
T19525 41942-41943 NNP denotes [
T19526 41943-41945 CD denotes 50
T19527 41945-41946 NNP denotes ]
T19528 41947-41951 VBD denotes were
T19529 41952-41960 VBN denotes cultured
T19530 41961-41963 IN denotes in
T19531 41964-41968 NNP denotes DMEM
T19532 41969-41975 NN denotes medium
T19533 41976-41988 VBN denotes supplemented
T19534 41989-41993 IN denotes with
T19535 41994-41996 CD denotes 10
T19536 41996-41997 NN denotes %
T19537 41998-42001 NNP denotes FBS
T19538 42002-42005 CC denotes and
T19539 42006-42028 JJ denotes antibiotic-antimycotic
T19540 42029-42037 NN denotes solution
T19541 42037-42038 . denotes .
T19666 42040-42052 NN denotes Purification
T19667 42053-42055 IN denotes of
T19668 42056-42066 NNP denotes Peripheral
T19669 42067-42072 NNP denotes Blood
T19670 42073-42082 NNPS denotes Monocytes
T19671 42083-42086 CC denotes and
T19672 42087-42103 NNP denotes Monocyte-Derived
T19673 42104-42115 NNP denotes Macrophages
T19674 42116-42117 -LRB- denotes (
T19675 42117-42121 NNP denotes MDMs
T19676 42121-42122 -RRB- denotes )
T19677 42123-42132 NNPS denotes Monocytes
T19678 42133-42137 VBD denotes were
T19679 42138-42146 VBN denotes isolated
T19680 42147-42151 IN denotes from
T19681 42152-42158 NN denotes source
T19682 42159-42168 NN denotes leukocyte
T19683 42169-42174 VBZ denotes packs
T19684 42175-42183 VBN denotes obtained
T19685 42184-42188 IN denotes from
T19686 42189-42192 DT denotes the
T19687 42193-42201 NNP denotes American
T19688 42202-42205 NNP denotes Red
T19689 42206-42211 NNP denotes Cross
T19690 42212-42214 IN denotes as
T19691 42215-42224 VBN denotes described
T19692 42225-42235 RB denotes previously
T19693 42236-42237 NNP denotes [
T19694 42237-42239 CD denotes 51
T19695 42239-42240 NNP denotes ]
T19696 42240-42241 . denotes .
T19697 42242-42251 NNS denotes Monocytes
T19698 42252-42256 VBN denotes used
T19699 42257-42259 IN denotes in
T19700 42260-42264 JJ denotes real
T19701 42265-42269 NN denotes time
T19702 42270-42273 NNP denotes PCR
T19703 42274-42285 NNS denotes experiments
T19704 42286-42289 CC denotes and
T19705 42290-42302 NN denotes transfection
T19706 42303-42314 NNS denotes experiments
T19707 42315-42319 VBD denotes were
T19708 42320-42328 VBN denotes purified
T19709 42329-42331 IN denotes by
T19710 42332-42340 JJ denotes positive
T19711 42341-42350 NN denotes selection
T19712 42351-42356 VBG denotes using
T19713 42357-42361 NNP denotes CD14
T19714 42362-42370 NNP denotes Monocyte
T19715 42371-42380 NNP denotes Isolation
T19716 42381-42384 NNP denotes Kit
T19717 42385-42389 IN denotes from
T19718 42390-42398 NNP denotes Miltenyi
T19719 42399-42406 NNP denotes Biotech
T19720 42407-42408 -LRB- denotes (
T19721 42408-42414 NNP denotes Auburn
T19722 42414-42415 , denotes ,
T19723 42416-42418 NNP denotes CA
T19724 42418-42419 -RRB- denotes )
T19725 42420-42421 -LRB- denotes (
T19726 42421-42422 FW denotes >
T19727 42422-42424 CD denotes 90
T19728 42424-42425 NN denotes %
T19729 42426-42430 JJ denotes pure
T19730 42430-42431 -RRB- denotes )
T19731 42431-42432 . denotes .
T19732 42433-42435 IN denotes In
T19733 42436-42440 DT denotes some
T19734 42441-42452 NNS denotes experiments
T19735 42452-42453 , denotes ,
T19736 42454-42463 NNS denotes monocytes
T19737 42464-42468 VBD denotes were
T19738 42469-42477 VBN denotes isolated
T19739 42478-42480 IN denotes by
T19740 42481-42489 VBG denotes clumping
T19741 42490-42496 NN denotes method
T19742 42497-42498 -LRB- denotes (
T19743 42498-42500 CD denotes 70
T19744 42500-42501 NN denotes %
T19745 42502-42506 JJ denotes pure
T19746 42506-42507 -RRB- denotes )
T19747 42507-42508 . denotes .
T19748 42509-42511 TO denotes To
T19749 42512-42518 VB denotes obtain
T19750 42519-42535 JJ denotes monocyte-derived
T19751 42536-42547 NNS denotes macrophages
T19752 42548-42549 -LRB- denotes (
T19753 42549-42553 NNS denotes MDMs
T19754 42553-42554 -RRB- denotes )
T19755 42554-42555 , denotes ,
T19756 42556-42565 NNS denotes monocytes
T19757 42566-42570 VBD denotes were
T19758 42571-42579 VBN denotes cultured
T19759 42580-42582 IN denotes in
T19760 42583-42592 NN denotes RPMI-1640
T19761 42593-42599 NN denotes medium
T19762 42600-42610 VBG denotes containing
T19763 42611-42613 CD denotes 10
T19764 42613-42614 NN denotes %
T19765 42615-42618 NNP denotes FBS
T19766 42618-42619 , denotes ,
T19767 42620-42623 CC denotes and
T19768 42624-42626 CD denotes 10
T19769 42627-42629 NN denotes µg
T19770 42629-42630 NN denotes /
T19771 42630-42632 NN denotes ml
T19772 42633-42642 NN denotes polymyxin
T19773 42643-42644 NNP denotes B
T19774 42645-42648 CC denotes and
T19775 42649-42651 CD denotes 20
T19776 42652-42657 JJ denotes ng/ml
T19777 42658-42663 NN denotes M-CSF
T19778 42663-42664 . denotes .
T19917 42666-42681 NNP denotes Electrophoresis
T19918 42682-42684 IN denotes of
T19919 42685-42688 DT denotes the
T19920 42689-42697 NNP denotes Mobility
T19921 42698-42703 NNP denotes Shift
T19922 42704-42712 NNP denotes Analysis
T19923 42713-42714 -LRB- denotes (
T19924 42714-42718 NNP denotes EMSA
T19925 42718-42719 -RRB- denotes )
T19926 42720-42727 NNP denotes Nuclear
T19927 42728-42736 NNS denotes extracts
T19928 42737-42741 VBD denotes were
T19929 42742-42750 VBN denotes isolated
T19930 42751-42755 IN denotes from
T19931 42756-42760 NNS denotes MDMs
T19932 42761-42763 IN denotes by
T19933 42764-42769 VBG denotes using
T19934 42770-42771 DT denotes a
T19935 42772-42779 JJ denotes nuclear
T19936 42780-42790 NN denotes extraction
T19937 42791-42794 NN denotes kit
T19938 42795-42804 VBG denotes according
T19939 42805-42807 TO denotes to
T19940 42808-42811 DT denotes the
T19941 42812-42824 NN denotes manufacturer
T19942 42824-42826 POS denotes 's
T19943 42827-42838 NN denotes instruction
T19944 42839-42843 IN denotes from
T19945 42844-42850 NNP denotes Active
T19946 42851-42856 NNP denotes Motif
T19947 42857-42858 -LRB- denotes (
T19948 42858-42866 NNP denotes Carlsbad
T19949 42866-42867 , denotes ,
T19950 42868-42870 NNP denotes CA
T19951 42870-42871 -RRB- denotes )
T19952 42871-42872 . denotes .
T19953 42873-42880 RB denotes Briefly
T19954 42880-42881 , denotes ,
T19955 42882-42886 NNS denotes MDMs
T19956 42887-42891 VBD denotes were
T19957 42892-42897 VBN denotes lysed
T19958 42898-42900 IN denotes in
T19959 42901-42910 JJ denotes hypotonic
T19960 42911-42916 NN denotes lysis
T19961 42917-42923 NN denotes buffer
T19962 42924-42925 -LRB- denotes (
T19963 42925-42927 CD denotes 20
T19964 42928-42930 NNP denotes mM
T19965 42931-42936 NNP denotes Hepes
T19966 42936-42937 , denotes ,
T19967 42938-42940 NNP denotes pH
T19968 42941-42944 CD denotes 7.5
T19969 42944-42945 : denotes ;
T19970 42946-42947 CD denotes 5
T19971 42948-42950 NNP denotes mM
T19972 42951-42954 NNP denotes NaF
T19973 42954-42955 , denotes ,
T19974 42956-42958 CD denotes 10
T19975 42959-42961 NNP denotes µM
T19976 42962-42969 NNP denotes Na2MoO4
T19977 42970-42973 CD denotes 0.5
T19978 42973-42974 NN denotes %
T19979 42975-42980 NN denotes NP-40
T19980 42981-42984 CC denotes and
T19981 42985-42988 CD denotes 0.1
T19982 42989-42991 NNP denotes mM
T19983 42992-42996 NNP denotes EDTA
T19984 42996-42997 -RRB- denotes )
T19985 42997-42998 , denotes ,
T19986 42999-43002 CC denotes and
T19987 43003-43007 RB denotes then
T19988 43008-43014 NNS denotes nuclei
T19989 43015-43019 VBD denotes were
T19990 43020-43031 VBN denotes resuspended
T19991 43032-43034 IN denotes in
T19992 43035-43040 NN denotes lysis
T19993 43041-43047 NN denotes buffer
T19994 43048-43060 VBN denotes supplemented
T19995 43061-43065 IN denotes with
T19996 43066-43069 CD denotes 0.5
T19997 43070-43072 NNP denotes mM
T19998 43073-43076 NNP denotes DTT
T19999 43077-43080 CC denotes and
T20000 43081-43084 CD denotes 0.2
T20001 43085-43087 NNP denotes mM
T20002 43088-43092 NNP denotes PMSF
T20003 43092-43093 . denotes .
T20004 43094-43097 DT denotes The
T20005 43098-43103 JJ denotes NF-κB
T20006 43104-43113 NN denotes consensus
T20007 43114-43130 NNS denotes oligonucleotides
T20008 43131-43132 -LRB- denotes (
T20009 43132-43137 NN denotes sense
T20010 43137-43138 : denotes :
T20011 43139-43161 NNP denotes AGTTGAGGGGACTTTCCCAGGC
T20012 43161-43162 : denotes ;
T20013 43163-43172 NN denotes antisense
T20014 43172-43173 : denotes :
T20015 43174-43196 NNP denotes GCCTGGGAAAGTCCCCTCAACT
T20016 43196-43197 -RRB- denotes )
T20017 43198-43205 VBN denotes labeled
T20018 43206-43210 IN denotes with
T20019 43211-43214 CD denotes 32P
T20020 43215-43217 IN denotes by
T20021 43218-43220 CD denotes T4
T20022 43221-43237 NN denotes polynucleokinase
T20023 43238-43239 -LRB- denotes (
T20024 43239-43246 NNP denotes Promega
T20025 43246-43247 , denotes ,
T20026 43248-43255 NNP denotes Madison
T20027 43255-43256 , denotes ,
T20028 43257-43259 NNP denotes WI
T20029 43259-43260 -RRB- denotes )
T20030 43261-43265 VBD denotes were
T20031 43266-43275 VBN denotes incubated
T20032 43276-43280 IN denotes with
T20033 43281-43288 JJ denotes nuclear
T20034 43289-43297 NNS denotes extracts
T20035 43298-43300 IN denotes in
T20036 43301-43308 JJ denotes binding
T20037 43309-43315 NN denotes buffer
T20038 43316-43317 -LRB- denotes (
T20039 43317-43319 CD denotes 10
T20040 43320-43322 NNP denotes mM
T20041 43323-43327 NNP denotes Tris
T20042 43328-43330 NNP denotes pH
T20043 43331-43334 CD denotes 7.6
T20044 43334-43335 , denotes ,
T20045 43336-43337 CD denotes 1
T20046 43338-43340 NNP denotes mM
T20047 43341-43344 NNP denotes DTT
T20048 43344-43345 , denotes ,
T20049 43346-43349 CD denotes 0.5
T20050 43350-43352 NNP denotes mM
T20051 43353-43357 NNP denotes EDTA
T20052 43357-43358 , denotes ,
T20053 43359-43360 CD denotes 2
T20054 43361-43363 NN denotes µg
T20055 43364-43373 NN denotes polydI-dC
T20056 43374-43377 CC denotes and
T20057 43378-43380 CD denotes 10
T20058 43380-43381 NN denotes %
T20059 43382-43390 NNP denotes Glycerol
T20060 43390-43391 -RRB- denotes )
T20061 43392-43394 IN denotes at
T20062 43395-43397 CD denotes 30
T20063 43397-43398 CD denotes °
T20064 43398-43399 NNP denotes C
T20065 43400-43403 IN denotes for
T20066 43404-43406 CD denotes 30
T20067 43407-43414 NNS denotes minutes
T20068 43414-43415 . denotes .
T20069 43416-43419 DT denotes The
T20070 43420-43424 JJ denotes free
T20071 43425-43428 NN denotes DNA
T20072 43429-43432 CC denotes and
T20073 43433-43444 JJ denotes DNA-protein
T20074 43445-43453 NNS denotes mixtures
T20075 43454-43458 VBD denotes were
T20076 43459-43467 VBN denotes resolved
T20077 43468-43470 IN denotes in
T20078 43471-43472 CD denotes 5
T20079 43472-43473 NN denotes %
T20080 43474-43480 JJ denotes native
T20081 43481-43495 NN denotes polyacrylamide
T20082 43496-43500 NNS denotes gels
T20083 43501-43503 IN denotes in
T20084 43504-43507 CD denotes 0.5
T20085 43507-43508 CD denotes ×
T20086 43509-43512 NNP denotes TBE
T20087 43513-43519 NN denotes buffer
T20088 43520-43521 -LRB- denotes (
T20089 43521-43523 CD denotes 45
T20090 43524-43526 NNP denotes mM
T20091 43527-43531 NNP denotes Tris
T20092 43531-43532 , denotes ,
T20093 43533-43535 CD denotes 45
T20094 43536-43538 NN denotes mM
T20095 43539-43544 JJ denotes boric
T20096 43545-43549 NN denotes acid
T20097 43550-43553 CC denotes and
T20098 43554-43555 CD denotes 1
T20099 43556-43558 NNP denotes mM
T20100 43559-43563 NNP denotes EDTA
T20101 43563-43564 , denotes ,
T20102 43565-43567 NNP denotes pH
T20103 43568-43571 CD denotes 8.3
T20104 43571-43572 -RRB- denotes )
T20105 43573-43575 IN denotes by
T20106 43576-43591 NN denotes electrophoresis
T20107 43591-43592 . denotes .
T20108 43593-43596 DT denotes The
T20109 43597-43600 NN denotes gel
T20110 43601-43604 VBD denotes was
T20111 43605-43610 VBN denotes dried
T20112 43611-43614 CC denotes and
T20113 43615-43624 VBN denotes subjected
T20114 43625-43627 TO denotes to
T20115 43628-43643 NN denotes autoradiography
T20116 43644-43652 NN denotes analysis
T20117 43652-43653 . denotes .
T20349 43655-43664 NNP denotes Transient
T20350 43665-43677 NNP denotes Transfection
T20351 43678-43680 IN denotes of
T20352 43681-43684 NNP denotes RAW
T20353 43685-43690 CD denotes 264.7
T20354 43691-43696 NNP denotes Cells
T20355 43697-43700 NNP denotes RAW
T20356 43701-43706 CD denotes 264.7
T20357 43707-43712 NNS denotes cells
T20358 43713-43717 VBD denotes were
T20359 43718-43724 VBN denotes seeded
T20360 43725-43727 IN denotes in
T20361 43728-43735 JJ denotes 12-well
T20362 43736-43742 NNS denotes plates
T20363 43743-43745 IN denotes in
T20364 43746-43750 NNP denotes RPMI
T20365 43751-43757 NN denotes medium
T20366 43758-43768 VBG denotes containing
T20367 43769-43770 CD denotes 5
T20368 43770-43771 NN denotes %
T20369 43772-43775 NNP denotes FBS
T20370 43776-43779 CD denotes one
T20371 43780-43783 NN denotes day
T20372 43784-43789 RB denotes prior
T20373 43790-43792 TO denotes to
T20374 43793-43805 NN denotes transfection
T20375 43805-43806 , denotes ,
T20376 43807-43815 NNP denotes Secreted
T20377 43816-43824 NN denotes alkaline
T20378 43825-43836 NN denotes phosphatase
T20379 43837-43838 -LRB- denotes (
T20380 43838-43842 NNP denotes SEAP
T20381 43842-43843 -RRB- denotes )
T20382 43844-43852 NN denotes reporter
T20383 43853-43861 NNS denotes plasmids
T20384 43862-43871 JJ denotes pTAL-SEAP
T20385 43872-43874 CC denotes or
T20386 43875-43886 JJ denotes pNF-κB-SEAP
T20387 43887-43891 VBD denotes were
T20388 43892-43903 VBN denotes transfected
T20389 43904-43908 IN denotes into
T20390 43909-43914 NNS denotes cells
T20391 43915-43920 VBG denotes using
T20392 43921-43928 NNP denotes Qiagene
T20393 43929-43938 NNP denotes Effectene
T20394 43939-43941 CC denotes or
T20395 43942-43952 NNP denotes Attractene
T20396 43953-43965 NN denotes transfection
T20397 43966-43969 NN denotes kit
T20398 43970-43971 -LRB- denotes (
T20399 43971-43977 NNP denotes Qiagen
T20400 43977-43978 , denotes ,
T20401 43979-43987 NNP denotes Valencia
T20402 43987-43988 , denotes ,
T20403 43989-43991 NNP denotes CA
T20404 43991-43992 -RRB- denotes )
T20405 43993-44002 VBG denotes according
T20406 44003-44005 TO denotes to
T20407 44006-44009 DT denotes the
T20408 44010-44022 NN denotes manufacturer
T20409 44022-44024 POS denotes 's
T20410 44025-44033 NN denotes protocol
T20411 44033-44034 . denotes .
T20412 44035-44038 IN denotes For
T20413 44039-44054 JJ denotes co-transfection
T20414 44055-44062 NNS denotes studies
T20415 44062-44063 , denotes ,
T20416 44064-44068 NNP denotes PKCα
T20417 44069-44071 CC denotes or
T20418 44072-44077 NNP denotes NF-κB
T20419 44078-44081 CD denotes p65
T20420 44082-44092 NNS denotes constructs
T20421 44093-44097 VBD denotes were
T20422 44098-44103 VBN denotes mixed
T20423 44104-44106 IN denotes at
T20424 44107-44108 DT denotes a
T20425 44109-44114 NN denotes ratio
T20426 44115-44117 IN denotes of
T20427 44118-44119 CD denotes 5
T20428 44119-44120 CD denotes ∶
T20429 44120-44121 CD denotes 1
T20430 44122-44126 IN denotes with
T20431 44127-44130 DT denotes the
T20432 44131-44139 NN denotes reporter
T20433 44140-44147 NN denotes plasmid
T20434 44147-44148 . denotes .
T20435 44149-44152 IN denotes For
T20436 44153-44158 JJ denotes siRNA
T20437 44159-44171 NN denotes transfection
T20438 44171-44172 , denotes ,
T20439 44173-44176 CD denotes 100
T20440 44177-44179 NN denotes nM
T20441 44180-44182 IN denotes of
T20442 44183-44187 NNP denotes PKCα
T20443 44188-44193 NNP denotes siRNA
T20444 44194-44196 CC denotes or
T20445 44197-44204 VB denotes control
T20446 44205-44210 NNP denotes siRNA
T20447 44211-44215 VBD denotes were
T20448 44216-44220 VBN denotes used
T20449 44220-44221 . denotes .
T20450 44222-44227 NNS denotes Cells
T20451 44228-44232 VBD denotes were
T20452 44233-44242 VBN denotes incubated
T20453 44243-44247 IN denotes with
T20454 44248-44251 DT denotes the
T20455 44252-44264 NN denotes transfection
T20456 44265-44273 VBZ denotes reagents
T20457 44274-44277 IN denotes for
T20458 44278-44280 CD denotes 16
T20459 44281-44283 CD denotes 24
T20460 44284-44289 NNS denotes hours
T20461 44289-44290 , denotes ,
T20462 44291-44297 VBD denotes washed
T20463 44298-44301 CC denotes and
T20464 44302-44309 VBD denotes starved
T20465 44310-44312 IN denotes in
T20466 44313-44317 NNP denotes RPMI
T20467 44318-44325 IN denotes without
T20468 44326-44329 NNP denotes FBS
T20469 44330-44333 IN denotes for
T20470 44334-44335 CD denotes 4
T20471 44336-44341 NNS denotes hours
T20472 44341-44342 . denotes .
T20473 44343-44350 NNS denotes Samples
T20474 44351-44355 VBD denotes were
T20475 44356-44360 RB denotes then
T20476 44361-44371 VBN denotes stimulated
T20477 44372-44376 IN denotes with
T20478 44377-44380 CD denotes 100
T20479 44381-44386 JJ denotes ng/ml
T20480 44387-44392 NN denotes M-CSF
T20481 44393-44396 IN denotes for
T20482 44397-44398 CD denotes 4
T20483 44399-44404 NNS denotes hours
T20484 44405-44407 IN denotes at
T20485 44408-44413 WDT denotes which
T20486 44414-44419 VBP denotes point
T20487 44420-44423 DT denotes the
T20488 44424-44430 NN denotes medium
T20489 44431-44434 VBD denotes was
T20490 44435-44444 VBN denotes collected
T20491 44445-44448 IN denotes for
T20492 44449-44452 DT denotes the
T20493 44453-44457 NNP denotes SEAP
T20494 44458-44466 NN denotes analysis
T20495 44466-44467 . denotes .
T20496 44468-44471 IN denotes For
T20497 44472-44482 NN denotes inhibition
T20498 44483-44490 NNS denotes studies
T20499 44490-44491 , denotes ,
T20500 44492-44497 NNS denotes cells
T20501 44498-44502 VBD denotes were
T20502 44503-44516 VBN denotes pre-incubated
T20503 44517-44521 IN denotes with
T20504 44522-44532 NNS denotes inhibitors
T20505 44533-44536 IN denotes for
T20506 44537-44539 CD denotes 30
T20507 44540-44547 NNS denotes minutes
T20508 44548-44554 IN denotes before
T20509 44555-44560 NNP denotes M-CSF
T20510 44561-44570 NN denotes treatment
T20511 44570-44571 . denotes .
T20512 44572-44574 IN denotes On
T20513 44575-44582 NN denotes average
T20514 44582-44583 , denotes ,
T20515 44584-44586 PRP denotes we
T20516 44587-44595 VBD denotes realized
T20517 44596-44598 CD denotes 30
T20518 44599-44601 CD denotes 40
T20519 44601-44602 NN denotes %
T20520 44603-44615 NN denotes transfection
T20521 44616-44626 NN denotes efficiency
T20522 44627-44629 IN denotes as
T20523 44630-44639 VBN denotes confirmed
T20524 44640-44642 IN denotes by
T20525 44643-44646 NNP denotes GFP
T20526 44647-44657 NN denotes expression
T20527 44658-44660 IN denotes in
T20528 44661-44664 NNP denotes Raw
T20529 44665-44670 CD denotes 264.7
T20530 44671-44676 NNS denotes cells
T20531 44676-44677 . denotes .
T20824 44679-44688 NNP denotes Transient
T20825 44689-44701 NNP denotes Transfection
T20826 44702-44704 IN denotes of
T20827 44705-44712 JJ denotes Primary
T20828 44713-44718 JJ denotes Human
T20829 44719-44728 NNP denotes Monocytes
T20830 44729-44738 NNPS denotes Monocytes
T20831 44739-44743 VBD denotes were
T20832 44744-44755 VBN denotes transfected
T20833 44756-44761 VBG denotes using
T20834 44762-44765 DT denotes the
T20835 44766-44771 JJ denotes human
T20836 44772-44780 NN denotes monocyte
T20837 44781-44786 NNP denotes Amaxa
T20838 44787-44799 NN denotes nucleofector
T20839 44800-44803 NN denotes kit
T20840 44804-44805 -LRB- denotes (
T20841 44805-44810 NNP denotes Lonza
T20842 44811-44823 NNP denotes Walkersville
T20843 44824-44827 NNP denotes Inc
T20844 44827-44828 , denotes ,
T20845 44829-44841 NNP denotes Walkersville
T20846 44841-44842 , denotes ,
T20847 44843-44845 NNP denotes MD
T20848 44845-44846 -RRB- denotes )
T20849 44847-44849 IN denotes as
T20850 44850-44860 RB denotes previously
T20851 44861-44870 VBN denotes described
T20852 44871-44872 NNP denotes [
T20853 44872-44874 CD denotes 51
T20854 44874-44875 NNP denotes ]
T20855 44875-44876 . denotes .
T20856 44877-44884 RB denotes Briefly
T20857 44884-44885 , denotes ,
T20858 44886-44895 NNS denotes monocytes
T20859 44896-44900 VBD denotes were
T20860 44901-44912 VBN denotes resuspended
T20861 44913-44915 IN denotes in
T20862 44916-44921 NNP denotes Amaxa
T20863 44922-44934 NNP denotes Nucleofactor
T20864 44935-44943 NN denotes solution
T20865 44944-44946 IN denotes at
T20866 44947-44948 DT denotes a
T20867 44949-44956 NN denotes density
T20868 44957-44959 IN denotes of
T20869 44960-44962 CD denotes 20
T20870 44962-44963 CD denotes ×
T20871 44963-44966 CD denotes 106
T20872 44967-44975 NN denotes cells/ml
T20873 44975-44976 , denotes ,
T20874 44977-44980 CC denotes and
T20875 44981-44982 CD denotes 2
T20876 44983-44985 NN denotes µg
T20877 44986-44988 IN denotes of
T20878 44989-44994 JJ denotes total
T20879 44995-45002 JJ denotes plasmid
T20880 45003-45006 NN denotes DNA
T20881 45007-45010 CD denotes 100
T20882 45011-45013 NNP denotes nM
T20883 45014-45016 IN denotes of
T20884 45017-45021 NNP denotes PKCα
T20885 45022-45027 NNP denotes siRNA
T20886 45028-45030 CC denotes or
T20887 45031-45038 VB denotes control
T20888 45039-45044 NNP denotes siRNA
T20889 45045-45048 VBD denotes was
T20890 45049-45054 VBN denotes added
T20891 45055-45058 CC denotes and
T20892 45059-45070 VBN denotes transfected
T20893 45071-45076 VBG denotes using
T20894 45077-45084 NN denotes program
T20895 45085-45090 NNP denotes Amaxa
T20896 45091-45095 NNP denotes Y-01
T20897 45095-45096 . denotes .
T20898 45097-45100 IN denotes For
T20899 45101-45116 JJ denotes co-transfection
T20900 45117-45124 NNS denotes studies
T20901 45124-45125 , denotes ,
T20902 45126-45130 NNP denotes PKCα
T20903 45131-45133 CC denotes or
T20904 45134-45139 NNP denotes NF-κB
T20905 45140-45143 CD denotes p65
T20906 45144-45154 NNS denotes constructs
T20907 45155-45159 VBD denotes were
T20908 45160-45165 VBN denotes mixed
T20909 45166-45168 IN denotes at
T20910 45169-45170 DT denotes a
T20911 45171-45176 NN denotes ratio
T20912 45177-45179 IN denotes of
T20913 45180-45181 CD denotes 5
T20914 45181-45182 CD denotes ∶
T20915 45182-45183 CD denotes 1
T20916 45184-45188 IN denotes with
T20917 45189-45192 DT denotes the
T20918 45193-45201 NN denotes reporter
T20919 45202-45209 NN denotes plasmid
T20920 45209-45210 . denotes .
T20921 45211-45222 RB denotes Immediately
T20922 45223-45228 IN denotes after
T20923 45229-45241 NN denotes transfection
T20924 45241-45242 , denotes ,
T20925 45243-45248 NNS denotes cells
T20926 45249-45253 VBD denotes were
T20927 45254-45260 VBN denotes washed
T20928 45261-45265 IN denotes with
T20929 45266-45267 CD denotes 1
T20930 45268-45270 NN denotes ml
T20931 45271-45273 IN denotes of
T20932 45274-45278 NNP denotes RPMI
T20933 45279-45285 NN denotes medium
T20934 45286-45296 VBG denotes containing
T20935 45297-45298 CD denotes 2
T20936 45299-45301 NNP denotes mM
T20937 45302-45311 NN denotes glutamine
T20938 45312-45315 CC denotes and
T20939 45316-45318 CD denotes 10
T20940 45318-45319 NN denotes %
T20941 45320-45323 NNP denotes FBS
T20942 45324-45326 CC denotes or
T20943 45327-45332 NN denotes serum
T20944 45333-45337 JJ denotes free
T20945 45338-45341 NNP denotes LGM
T20946 45342-45348 NN denotes medium
T20947 45349-45350 -LRB- denotes (
T20948 45350-45355 NNP denotes Lonza
T20949 45356-45368 NNP denotes Walkersville
T20950 45369-45373 NNP denotes Inc.
T20951 45373-45374 -RRB- denotes )
T20952 45375-45379 RB denotes then
T20953 45380-45386 VBD denotes plated
T20954 45387-45389 IN denotes at
T20955 45390-45391 CD denotes 4
T20956 45391-45392 CD denotes ×
T20957 45392-45395 CD denotes 105
T20958 45396-45406 NN denotes cells/well
T20959 45407-45409 IN denotes in
T20960 45410-45417 JJ denotes 24-well
T20961 45418-45424 NNS denotes plates
T20962 45424-45425 . denotes .
T20963 45426-45429 DT denotes The
T20964 45430-45438 JJ denotes original
T20965 45439-45445 NN denotes medium
T20966 45446-45449 VBD denotes was
T20967 45450-45458 VBN denotes replaced
T20968 45459-45461 IN denotes by
T20969 45462-45467 NN denotes serum
T20970 45468-45472 JJ denotes free
T20971 45473-45476 NNP denotes LGM
T20972 45477-45483 NN denotes medium
T20973 45484-45491 IN denotes without
T20974 45492-45494 CC denotes or
T20975 45495-45499 IN denotes with
T20976 45500-45505 NNP denotes M-CSF
T20977 45506-45507 -LRB- denotes (
T20978 45507-45510 CD denotes 100
T20979 45511-45516 NN denotes ng/ml
T20980 45516-45517 -RRB- denotes )
T20981 45518-45521 CD denotes one
T20982 45522-45526 NN denotes hour
T20983 45527-45532 RB denotes later
T20984 45532-45533 . denotes .
T20985 45534-45537 DT denotes The
T20986 45538-45542 JJ denotes next
T20987 45543-45546 NN denotes day
T20988 45546-45547 , denotes ,
T20989 45548-45557 JJ denotes cell-free
T20990 45558-45570 NNS denotes supernatants
T20991 45571-45575 VBD denotes were
T20992 45576-45585 VBN denotes collected
T20993 45586-45589 IN denotes for
T20994 45590-45593 DT denotes the
T20995 45594-45598 NNP denotes SEAP
T20996 45599-45607 NN denotes analysis
T20997 45607-45608 . denotes .
T20998 45609-45614 NNS denotes Cells
T20999 45615-45619 VBD denotes were
T21000 45620-45627 VBN denotes stained
T21001 45628-45632 IN denotes with
T21002 45633-45640 NNP denotes Annexin
T21003 45641-45652 NNP denotes V/propidium
T21004 45653-45659 NN denotes iodide
T21005 45660-45661 -LRB- denotes (
T21006 45661-45663 NNP denotes PI
T21007 45663-45664 -RRB- denotes )
T21008 45664-45665 . denotes .
T21009 45666-45669 IN denotes For
T21010 45670-45673 DT denotes the
T21011 45674-45684 NN denotes inhibition
T21012 45685-45692 NNS denotes studies
T21013 45692-45693 , denotes ,
T21014 45694-45699 NNS denotes cells
T21015 45700-45704 VBD denotes were
T21016 45705-45718 VBN denotes pre-incubated
T21017 45719-45723 IN denotes with
T21018 45724-45733 NN denotes inhibitor
T21019 45734-45737 IN denotes for
T21020 45738-45740 CD denotes 30
T21021 45741-45748 NNS denotes minutes
T21022 45749-45751 IN denotes in
T21023 45752-45758 NNP denotes X-vivo
T21024 45759-45765 NN denotes medium
T21025 45766-45771 RB denotes prior
T21026 45772-45774 TO denotes to
T21027 45775-45778 DT denotes the
T21028 45779-45787 NN denotes addition
T21029 45788-45790 IN denotes of
T21030 45791-45796 NNP denotes M-CSF
T21031 45796-45797 . denotes .
T21302 45799-45807 NNP denotes Secreted
T21303 45808-45816 NNP denotes Alkaline
T21304 45817-45828 NNP denotes Phosphatase
T21305 45829-45830 -LRB- denotes (
T21306 45830-45834 NNP denotes SEAP
T21307 45834-45835 -RRB- denotes )
T21308 45836-45844 NNP denotes Analysis
T21309 45845-45853 NNP denotes Secreted
T21310 45854-45862 NN denotes alkaline
T21311 45863-45874 NN denotes phosphatase
T21312 45875-45883 NN denotes activity
T21313 45884-45887 VBD denotes was
T21314 45888-45896 VBN denotes analyzed
T21315 45897-45899 IN denotes by
T21316 45900-45911 NNP denotes GreatEscape
T21317 45912-45916 NNP denotes SEAP
T21318 45917-45920 NN denotes kit
T21319 45921-45930 VBG denotes according
T21320 45931-45933 TO denotes to
T21321 45934-45937 DT denotes the
T21322 45938-45950 NN denotes manufacturer
T21323 45950-45952 POS denotes 's
T21324 45953-45964 NN denotes instruction
T21325 45964-45965 . denotes .
T21326 45966-45973 RB denotes Briefly
T21327 45973-45974 , denotes ,
T21328 45975-45981 NN denotes medium
T21329 45982-45985 VBD denotes was
T21330 45986-45993 VBN denotes diluted
T21331 45994-45996 IN denotes in
T21332 45997-46000 DT denotes the
T21333 46001-46009 NN denotes dilution
T21334 46010-46016 NN denotes buffer
T21335 46017-46020 CC denotes and
T21336 46021-46027 VBN denotes heated
T21337 46028-46030 TO denotes to
T21338 46031-46033 CD denotes 65
T21339 46033-46034 CD denotes °
T21340 46034-46035 NNP denotes C
T21341 46036-46038 TO denotes to
T21342 46039-46049 VB denotes inactivate
T21343 46050-46060 JJ denotes endogenous
T21344 46061-46073 NNS denotes phosphatases
T21345 46074-46078 RB denotes then
T21346 46079-46088 VBD denotes incubated
T21347 46089-46093 IN denotes with
T21348 46094-46097 DT denotes the
T21349 46098-46107 NN denotes substrate
T21350 46108-46111 IN denotes for
T21351 46112-46114 CD denotes 30
T21352 46115-46122 NNS denotes minutes
T21353 46122-46123 . denotes .
T21354 46124-46127 DT denotes The
T21355 46128-46142 NN denotes chemiluminence
T21356 46143-46149 NN denotes signal
T21357 46150-46153 VBD denotes was
T21358 46154-46162 VBN denotes recorded
T21359 46163-46168 VBG denotes using
T21360 46169-46170 DT denotes a
T21361 46171-46182 NN denotes luminometer
T21362 46182-46183 . denotes .
T21451 46185-46192 NNP denotes Annexin
T21452 46193-46197 NNP denotes V/PI
T21453 46198-46207 NNP denotes Apoptosis
T21454 46208-46213 NNP denotes Assay
T21455 46214-46218 NNP denotes Cell
T21456 46219-46228 NN denotes apoptosis
T21457 46229-46234 NN denotes assay
T21458 46235-46238 VBD denotes was
T21459 46239-46248 VBN denotes performed
T21460 46249-46251 IN denotes as
T21461 46252-46261 VBN denotes described
T21462 46262-46272 RB denotes previously
T21463 46273-46274 NNP denotes [
T21464 46274-46276 CD denotes 51
T21465 46276-46277 NNP denotes ]
T21466 46278-46283 VBG denotes using
T21467 46284-46287 DT denotes the
T21468 46288-46295 NNP denotes Annexin
T21469 46296-46297 NNP denotes V
T21470 46298-46302 NNP denotes FITC
T21471 46303-46312 VBZ denotes apoptosis
T21472 46313-46322 NN denotes detection
T21473 46323-46326 NN denotes kit
T21474 46327-46328 -LRB- denotes (
T21475 46328-46330 NNP denotes BD
T21476 46331-46341 NNP denotes PharMingen
T21477 46341-46342 , denotes ,
T21478 46343-46346 NNP denotes San
T21479 46347-46352 NNP denotes Diego
T21480 46352-46353 , denotes ,
T21481 46354-46356 NNP denotes CA
T21482 46356-46357 -RRB- denotes )
T21483 46357-46358 . denotes .
T21484 46359-46366 RB denotes Briefly
T21485 46366-46367 , denotes ,
T21486 46368-46373 JJ denotes human
T21487 46374-46383 NNS denotes monocytes
T21488 46384-46385 -LRB- denotes (
T21489 46385-46386 CD denotes 5
T21490 46386-46387 CD denotes ×
T21491 46387-46390 CD denotes 106
T21492 46390-46391 -RRB- denotes )
T21493 46392-46396 VBD denotes were
T21494 46397-46406 VBN denotes incubated
T21495 46407-46411 IN denotes with
T21496 46412-46422 NNS denotes inhibitors
T21497 46423-46426 IN denotes for
T21498 46427-46429 CD denotes 30
T21499 46430-46437 NNS denotes minutes
T21500 46438-46440 IN denotes in
T21501 46441-46447 NNP denotes X-vivo
T21502 46448-46454 NN denotes medium
T21503 46455-46458 CC denotes and
T21504 46459-46471 VBN denotes restimulated
T21505 46472-46476 IN denotes with
T21506 46477-46480 CD denotes 100
T21507 46481-46486 JJ denotes ng/ml
T21508 46487-46492 NNP denotes M-CSF
T21509 46493-46502 JJ denotes overnight
T21510 46502-46503 . denotes .
T21511 46504-46507 DT denotes The
T21512 46508-46513 NNS denotes cells
T21513 46514-46518 VBD denotes were
T21514 46519-46526 VBN denotes removed
T21515 46527-46531 IN denotes from
T21516 46532-46535 DT denotes the
T21517 46536-46543 NN denotes culture
T21518 46544-46548 NN denotes dish
T21519 46549-46554 VBG denotes using
T21520 46555-46563 NNP denotes Accutase
T21521 46564-46565 -LRB- denotes (
T21522 46565-46576 NNP denotes eBioscience
T21523 46576-46577 , denotes ,
T21524 46578-46581 NNP denotes San
T21525 46582-46587 NNP denotes Diego
T21526 46587-46588 , denotes ,
T21527 46589-46591 NNP denotes CA
T21528 46591-46592 -RRB- denotes )
T21529 46593-46596 CC denotes and
T21530 46597-46604 VBN denotes stained
T21531 46605-46609 IN denotes with
T21532 46610-46617 NNP denotes Annexin
T21533 46618-46619 NNP denotes V
T21534 46620-46624 NNP denotes FITC
T21535 46625-46628 CC denotes and
T21536 46629-46631 NNP denotes PI
T21537 46632-46635 CC denotes and
T21538 46636-46644 VBN denotes analyzed
T21539 46645-46647 IN denotes by
T21540 46648-46652 NN denotes flow
T21541 46653-46662 NN denotes cytometry
T21542 46663-46664 -LRB- denotes (
T21543 46664-46675 NNP denotes FACSCalibur
T21544 46675-46676 : denotes ;
T21545 46677-46679 NNP denotes BD
T21546 46680-46690 NNP denotes PharMingen
T21547 46690-46691 -RRB- denotes )
T21548 46691-46692 . denotes .
T21549 46693-46700 NNP denotes Annexin
T21550 46701-46707 NNP denotes V-FITC
T21551 46708-46711 CC denotes and
T21552 46712-46714 NNP denotes PI
T21553 46715-46721 JJ denotes double
T21554 46722-46730 JJ denotes negative
T21555 46731-46736 NNS denotes cells
T21556 46737-46741 VBD denotes were
T21557 46742-46752 VBN denotes considered
T21558 46753-46766 JJ denotes non-apoptotic
T21559 46767-46772 NNS denotes cells
T21560 46773-46776 IN denotes for
T21561 46777-46788 JJ denotes statistical
T21562 46789-46797 NN denotes analysis
T21563 46797-46798 . denotes .
T21735 46800-46807 JJ denotes Western
T21736 46808-46812 NNP denotes Blot
T21737 46813-46821 NNP denotes Analysis
T21738 46822-46827 NNPS denotes Cells
T21739 46828-46832 VBD denotes were
T21740 46833-46839 VBN denotes washed
T21741 46840-46844 IN denotes with
T21742 46845-46848 NNP denotes PBS
T21743 46849-46852 CC denotes and
T21744 46853-46864 VBD denotes resuspended
T21745 46865-46867 IN denotes in
T21746 46868-46872 NN denotes cell
T21747 46873-46878 NN denotes lysis
T21748 46879-46885 NN denotes buffer
T21749 46885-46886 . denotes .
T21750 46887-46888 -LRB- denotes (
T21751 46888-46892 NNP denotes Cell
T21752 46893-46902 NNP denotes Signaling
T21753 46903-46913 NNP denotes Technology
T21754 46913-46914 -RRB- denotes )
T21755 46915-46918 CC denotes and
T21756 46919-46928 VBN denotes incubated
T21757 46929-46932 IN denotes for
T21758 46933-46935 CD denotes 10
T21759 46936-46943 NNS denotes minutes
T21760 46944-46946 IN denotes on
T21761 46947-46950 NN denotes ice
T21762 46951-46955 RB denotes then
T21763 46956-46967 VBD denotes centrifuged
T21764 46968-46970 TO denotes to
T21765 46971-46977 VB denotes remove
T21766 46978-46981 DT denotes the
T21767 46982-46991 JJ denotes insoluble
T21768 46992-47000 NN denotes fraction
T21769 47000-47001 . denotes .
T21770 47002-47005 DT denotes The
T21771 47006-47013 NN denotes protein
T21772 47014-47027 NN denotes concentration
T21773 47028-47031 VBD denotes was
T21774 47032-47042 VBN denotes determined
T21775 47043-47045 IN denotes by
T21776 47046-47049 DT denotes the
T21777 47050-47053 NNP denotes BCA
T21778 47054-47061 NN denotes protein
T21779 47062-47067 NN denotes assay
T21780 47068-47069 -LRB- denotes (
T21781 47069-47076 NNP denotes Bio-Rad
T21782 47076-47077 , denotes ,
T21783 47078-47086 NNP denotes Hercules
T21784 47086-47087 , denotes ,
T21785 47088-47090 NNP denotes CA
T21786 47090-47091 -RRB- denotes )
T21787 47091-47092 . denotes .
T21788 47093-47097 NN denotes Cell
T21789 47098-47105 NNS denotes lysates
T21790 47106-47110 VBD denotes were
T21791 47111-47120 VBN denotes separated
T21792 47121-47123 IN denotes by
T21793 47124-47132 NNP denotes SDS-PAGE
T21794 47133-47135 IN denotes on
T21795 47136-47138 CD denotes 10
T21796 47138-47139 NN denotes %
T21797 47140-47154 NN denotes polyacrylamide
T21798 47155-47159 NNS denotes gels
T21799 47160-47163 CC denotes and
T21800 47164-47168 RB denotes then
T21801 47169-47180 VBD denotes transferred
T21802 47181-47185 IN denotes onto
T21803 47186-47200 JJ denotes nitrocellulose
T21804 47201-47210 NNS denotes membranes
T21805 47211-47214 CC denotes and
T21806 47215-47224 VBN denotes subjected
T21807 47225-47227 TO denotes to
T21808 47228-47235 JJ denotes Western
T21809 47236-47244 VBG denotes blotting
T21810 47244-47245 . denotes .
T21811 47246-47249 DT denotes The
T21812 47250-47263 JJ denotes immunoblotted
T21813 47264-47272 NNS denotes proteins
T21814 47273-47277 VBD denotes were
T21815 47278-47286 VBN denotes detected
T21816 47287-47289 IN denotes by
T21817 47290-47293 NNP denotes ECL
T21818 47294-47301 NN denotes reagent
T21819 47302-47303 -LRB- denotes (
T21820 47303-47305 NNP denotes GE
T21821 47306-47316 NNP denotes Healthcare
T21822 47317-47329 NNP denotes Bio-Sciences
T21823 47330-47335 NNP denotes Corp.
T21824 47335-47336 , denotes ,
T21825 47337-47347 NNP denotes Piscataway
T21826 47347-47348 , denotes ,
T21827 47349-47351 NNP denotes NJ
T21828 47351-47352 -RRB- denotes )
T21829 47352-47353 . denotes .
T21944 47355-47363 NN denotes Analysis
T21945 47364-47366 IN denotes of
T21946 47367-47371 NNP denotes PKCα
T21947 47372-47378 NNP denotes Kinase
T21948 47379-47387 NNP denotes Activity
T21949 47388-47393 NNP denotes Human
T21950 47394-47398 NNPS denotes MDMs
T21951 47399-47403 VBD denotes were
T21952 47404-47411 VBN denotes starved
T21953 47412-47415 IN denotes for
T21954 47416-47417 CD denotes 2
T21955 47418-47423 NNS denotes hours
T21956 47424-47430 IN denotes before
T21957 47431-47442 NN denotes stimulation
T21958 47443-47447 IN denotes with
T21959 47448-47453 NNP denotes M-CSF
T21960 47454-47455 -LRB- denotes (
T21961 47455-47456 CD denotes 0
T21962 47457-47459 CD denotes 60
T21963 47460-47467 NNS denotes minutes
T21964 47467-47468 -RRB- denotes )
T21965 47468-47469 , denotes ,
T21966 47470-47474 RB denotes then
T21967 47475-47481 VBD denotes washed
T21968 47482-47486 IN denotes with
T21969 47487-47491 JJ denotes cold
T21970 47492-47495 NNP denotes PBS
T21971 47496-47499 CC denotes and
T21972 47500-47507 VBN denotes removed
T21973 47508-47512 IN denotes from
T21974 47513-47516 DT denotes the
T21975 47517-47523 NNS denotes plates
T21976 47523-47524 . denotes .
T21977 47525-47528 DT denotes The
T21978 47529-47534 NNS denotes cells
T21979 47535-47539 VBD denotes were
T21980 47540-47551 VBN denotes resuspended
T21981 47552-47554 IN denotes in
T21982 47555-47560 NN denotes lysis
T21983 47561-47567 NN denotes buffer
T21984 47568-47569 -LRB- denotes (
T21985 47569-47571 CD denotes 20
T21986 47572-47574 NNP denotes mM
T21987 47575-47579 NNP denotes Tris
T21988 47579-47580 , denotes ,
T21989 47581-47582 CD denotes 5
T21990 47583-47585 NNP denotes mM
T21991 47586-47591 NNP denotes MgCl2
T21992 47591-47592 , denotes ,
T21993 47593-47594 CD denotes 1
T21994 47595-47597 NNP denotes mM
T21995 47598-47602 NNP denotes EGTA
T21996 47602-47603 , denotes ,
T21997 47604-47606 CD denotes 20
T21998 47607-47609 NNP denotes mM
T21999 47610-47620 JJ denotes β-glycerol
T22000 47621-47630 NN denotes phosphate
T22001 47630-47631 , denotes ,
T22002 47632-47633 CD denotes 1
T22003 47634-47636 NNP denotes mM
T22004 47637-47641 NNP denotes PMSF
T22005 47642-47643 CD denotes 2
T22006 47644-47646 NN denotes µg
T22007 47646-47647 NN denotes /
T22008 47647-47649 NN denotes ml
T22009 47650-47659 NN denotes aprotinin
T22010 47660-47663 CC denotes and
T22011 47664-47665 CD denotes 2
T22012 47666-47668 NN denotes µg
T22013 47668-47669 NN denotes /
T22014 47669-47671 NN denotes ml
T22015 47672-47681 NN denotes leupeptin
T22016 47681-47682 , denotes ,
T22017 47683-47685 NNP denotes pH
T22018 47686-47689 CD denotes 7.5
T22019 47689-47690 -RRB- denotes )
T22020 47690-47691 , denotes ,
T22021 47692-47701 VBN denotes sonicated
T22022 47702-47705 CC denotes and
T22023 47706-47717 VBN denotes centrifuged
T22024 47718-47720 TO denotes to
T22025 47721-47727 VB denotes obtain
T22026 47728-47733 JJ denotes whole
T22027 47734-47738 NN denotes cell
T22028 47739-47746 NNS denotes lysates
T22029 47746-47747 , denotes ,
T22030 47748-47753 WDT denotes which
T22031 47754-47758 VBD denotes were
T22032 47759-47763 RB denotes then
T22033 47764-47782 VBN denotes immunoprecipitated
T22034 47783-47787 IN denotes with
T22035 47788-47797 JJ denotes anti-PKCα
T22036 47798-47806 NN denotes antibody
T22037 47807-47810 CC denotes and
T22038 47811-47818 NN denotes protein
T22039 47819-47828 NN denotes G-agarose
T22040 47829-47830 -LRB- denotes (
T22041 47830-47840 NNP denotes Invitrogen
T22042 47840-47841 -RRB- denotes )
T22043 47841-47842 . denotes .
T22044 47843-47849 NNP denotes Immune
T22045 47850-47859 NNS denotes complexes
T22046 47860-47864 VBD denotes were
T22047 47865-47871 VBN denotes washed
T22048 47872-47877 RB denotes twice
T22049 47877-47878 , denotes ,
T22050 47879-47882 CC denotes and
T22051 47883-47887 NNP denotes PKCα
T22052 47888-47896 NN denotes activity
T22053 47897-47900 VBD denotes was
T22054 47901-47909 VBN denotes analyzed
T22055 47910-47912 IN denotes by
T22056 47913-47928 JJ denotes non-radioactive
T22057 47929-47935 NNP denotes Peptag
T22058 47936-47941 NN denotes assay
T22059 47942-47945 NN denotes kit
T22060 47946-47947 -LRB- denotes (
T22061 47947-47954 NNP denotes Promega
T22062 47954-47955 -RRB- denotes )
T22063 47955-47956 . denotes .
T22064 47957-47964 RB denotes Briefly
T22065 47964-47965 , denotes ,
T22066 47966-47972 NN denotes kinase
T22067 47973-47979 NN denotes buffer
T22068 47979-47980 , denotes ,
T22069 47981-47990 NN denotes activator
T22070 47990-47991 , denotes ,
T22071 47992-47999 NN denotes peptide
T22072 48000-48010 NN denotes protection
T22073 48011-48019 NN denotes solution
T22074 48020-48023 CC denotes and
T22075 48024-48030 NNP denotes Peptag
T22076 48031-48038 NN denotes peptide
T22077 48039-48043 VBD denotes were
T22078 48044-48053 VBN denotes incubated
T22079 48054-48058 IN denotes with
T22080 48059-48062 DT denotes the
T22081 48063-48069 JJ denotes immune
T22082 48070-48079 NNS denotes complexes
T22083 48080-48082 IN denotes at
T22084 48083-48085 CD denotes 30
T22085 48085-48086 CD denotes °
T22086 48086-48087 NNP denotes C
T22087 48088-48091 IN denotes for
T22088 48092-48094 CD denotes 30
T22089 48095-48102 NNS denotes minutes
T22090 48102-48103 . denotes .
T22091 48104-48113 NNS denotes Reactions
T22092 48114-48118 VBD denotes were
T22093 48119-48126 VBN denotes stopped
T22094 48127-48129 IN denotes by
T22095 48130-48137 VBG denotes boiling
T22096 48138-48141 IN denotes for
T22097 48142-48144 CD denotes 10
T22098 48145-48152 NNS denotes minutes
T22099 48153-48156 CC denotes and
T22100 48157-48164 NNS denotes samples
T22101 48165-48169 VBD denotes were
T22102 48170-48179 VBN denotes separated
T22103 48180-48182 IN denotes on
T22104 48183-48186 CD denotes 0.8
T22105 48186-48187 NN denotes %
T22106 48188-48195 JJ denotes agarose
T22107 48196-48199 NN denotes gel
T22108 48199-48200 . denotes .
T22109 48201-48215 NNP denotes Phosphorylated
T22110 48216-48223 NN denotes peptide
T22111 48224-48232 VBD denotes migrated
T22112 48233-48239 IN denotes toward
T22113 48240-48243 DT denotes the
T22114 48244-48249 NN denotes anode
T22115 48250-48251 -LRB- denotes (
T22116 48251-48252 FW denotes +
T22117 48252-48253 -RRB- denotes )
T22118 48253-48254 , denotes ,
T22119 48255-48260 IN denotes while
T22120 48261-48279 JJ denotes non-phosphorylated
T22121 48280-48287 NN denotes peptide
T22122 48288-48296 VBD denotes migrated
T22123 48297-48303 IN denotes toward
T22124 48304-48307 DT denotes the
T22125 48308-48315 NN denotes cathode
T22126 48316-48317 -LRB- denotes (
T22127 48317-48318 FW denotes −
T22128 48318-48319 -RRB- denotes )
T22129 48319-48320 . denotes .
T22130 48321-48339 JJ denotes Fluorescein-tagged
T22131 48340-48348 NNS denotes peptides
T22132 48349-48353 VBD denotes were
T22133 48354-48364 VBN denotes visualized
T22134 48365-48367 IN denotes by
T22135 48368-48370 NNP denotes UV
T22136 48371-48376 NN denotes light
T22137 48376-48377 . denotes .
T22378 48379-48382 NNP denotes RNA
T22379 48383-48392 NNP denotes Isolation
T22380 48393-48396 CC denotes and
T22381 48397-48409 JJ denotes Quantitative
T22382 48410-48419 JJ denotes Real-time
T22383 48420-48423 NNP denotes PCR
T22384 48424-48428 NNPS denotes MDMs
T22385 48429-48431 CC denotes or
T22386 48432-48435 NNP denotes RAW
T22387 48436-48441 CD denotes 264.7
T22388 48442-48447 NNS denotes cells
T22389 48448-48452 VBD denotes were
T22390 48453-48466 JJ denotes serum-starved
T22391 48467-48476 JJ denotes overnight
T22392 48477-48479 CC denotes or
T22393 48480-48483 IN denotes for
T22394 48484-48485 CD denotes 2
T22395 48486-48491 NNS denotes hours
T22396 48491-48492 , denotes ,
T22397 48493-48505 RB denotes respectively
T22398 48505-48506 , denotes ,
T22399 48507-48512 RB denotes prior
T22400 48513-48515 TO denotes to
T22401 48516-48526 VBG denotes incubating
T22402 48527-48531 IN denotes with
T22403 48532-48542 NNS denotes inhibitors
T22404 48543-48546 IN denotes for
T22405 48547-48549 CD denotes 30
T22406 48550-48557 NNS denotes minutes
T22407 48557-48558 . denotes .
T22408 48559-48562 DT denotes The
T22409 48563-48568 NNS denotes cells
T22410 48569-48573 VBD denotes were
T22411 48574-48586 VBN denotes restimulated
T22412 48587-48591 IN denotes with
T22413 48592-48595 CD denotes 100
T22414 48596-48601 JJ denotes ng/ml
T22415 48602-48607 NNP denotes M-CSF
T22416 48608-48611 CC denotes and
T22417 48612-48617 JJ denotes total
T22418 48618-48622 NNP denotes mRNA
T22419 48623-48626 VBD denotes was
T22420 48627-48636 VBN denotes extracted
T22421 48637-48641 IN denotes from
T22422 48642-48647 NNS denotes cells
T22423 48648-48652 IN denotes with
T22424 48653-48659 NNP denotes Trizol
T22425 48660-48661 -LRB- denotes (
T22426 48661-48671 NNP denotes Invitrogen
T22427 48671-48672 -RRB- denotes )
T22428 48673-48676 CC denotes and
T22429 48677-48678 CD denotes 1
T22430 48679-48680 CD denotes 2
T22431 48681-48683 NN denotes µg
T22432 48684-48686 IN denotes of
T22433 48687-48692 JJ denotes total
T22434 48693-48696 NNP denotes RNA
T22435 48697-48700 VBD denotes was
T22436 48701-48705 VBN denotes used
T22437 48706-48708 TO denotes to
T22438 48709-48720 VBN denotes synthesized
T22439 48721-48725 NNP denotes cDNA
T22440 48726-48731 VBG denotes using
T22441 48732-48743 NNP denotes SuperScript
T22442 48744-48747 NNP denotes III
T22443 48748-48749 -LRB- denotes (
T22444 48749-48759 NNP denotes Invitrogen
T22445 48759-48760 -RRB- denotes )
T22446 48760-48761 . denotes .
T22447 48762-48774 JJ denotes Quantitative
T22448 48775-48784 JJ denotes real-time
T22449 48785-48786 -LRB- denotes (
T22450 48786-48789 NNP denotes qRT
T22451 48789-48790 -RRB- denotes )
T22452 48790-48791 : denotes -
T22453 48791-48794 NNP denotes PCR
T22454 48795-48798 VBD denotes was
T22455 48799-48808 VBN denotes performed
T22456 48809-48814 VBG denotes using
T22457 48815-48819 NNP denotes SYBR
T22458 48820-48825 NNP denotes Green
T22459 48826-48832 NNP denotes Master
T22460 48833-48836 NNP denotes Mix
T22461 48837-48838 -LRB- denotes (
T22462 48838-48845 NNP denotes Applied
T22463 48846-48856 NNPS denotes BioSystems
T22464 48856-48857 , denotes ,
T22465 48858-48866 NNP denotes Carlsbad
T22466 48866-48867 , denotes ,
T22467 48868-48870 NNP denotes CA
T22468 48870-48871 -RRB- denotes )
T22469 48871-48872 . denotes .
T22470 48873-48876 DT denotes The
T22471 48877-48886 NNS denotes reactions
T22472 48887-48891 VBD denotes were
T22473 48892-48901 VBN denotes performed
T22474 48902-48907 VBG denotes using
T22475 48908-48910 DT denotes an
T22476 48911-48914 NNP denotes ABI
T22477 48915-48920 NNP denotes PRIZM
T22478 48921-48925 CD denotes 7700
T22479 48926-48933 NN denotes machine
T22480 48934-48938 IN denotes with
T22481 48939-48947 NN denotes software
T22482 48948-48956 NNP denotes Sequence
T22483 48957-48965 NNP denotes Detector
T22484 48966-48973 NN denotes version
T22485 48974-48977 CD denotes 1.7
T22486 48978-48979 -LRB- denotes (
T22487 48979-48986 NNP denotes Applied
T22488 48987-48997 NNPS denotes Biosystems
T22489 48997-48998 -RRB- denotes )
T22490 48998-48999 . denotes .
T22491 49000-49003 DT denotes The
T22492 49004-49010 NN denotes target
T22493 49011-49015 NN denotes gene
T22494 49016-49022 NNS denotes values
T22495 49023-49027 VBD denotes were
T22496 49028-49038 VBN denotes normalized
T22497 49039-49041 TO denotes to
T22498 49042-49045 DT denotes the
T22499 49046-49052 NNS denotes values
T22500 49053-49055 IN denotes of
T22501 49056-49061 NNP denotes GAPDH
T22502 49062-49064 IN denotes as
T22503 49065-49066 DT denotes a
T22504 49067-49079 NN denotes housekeeping
T22505 49080-49084 NN denotes gene
T22506 49085-49088 CC denotes and
T22507 49089-49098 VBD denotes expressed
T22508 49099-49101 IN denotes as
T22509 49102-49110 JJ denotes relative
T22510 49111-49115 VBP denotes fold
T22511 49116-49124 NN denotes increase
T22512 49125-49126 CD denotes 2
T22513 49126-49127 -LRB- denotes (
T22514 49127-49128 NN denotes −
T22515 49128-49132 NNP denotes ΔΔCt
T22516 49132-49133 -RRB- denotes )
T22517 49134-49138 IN denotes over
T22518 49139-49142 DT denotes the
T22519 49143-49157 JJ denotes non-stimulated
T22520 49158-49165 NNS denotes samples
T22521 49166-49167 -LRB- denotes (
T22522 49167-49169 NNP denotes NS
T22523 49169-49170 -RRB- denotes )
T22524 49170-49171 . denotes .
T22732 49395-49401 NNS denotes Ethics
T22733 49402-49411 NNP denotes Statement
T22734 49412-49415 NNP denotes All
T22735 49416-49424 NN denotes research
T22736 49425-49434 VBG denotes involving
T22737 49435-49440 JJ denotes human
T22738 49441-49446 NN denotes blood
T22739 49447-49448 -LRB- denotes (
T22740 49448-49453 NNP denotes Buffy
T22741 49454-49458 NN denotes coat
T22742 49458-49459 -RRB- denotes )
T22743 49460-49464 VBD denotes were
T22744 49465-49472 VBN denotes ordered
T22745 49473-49477 IN denotes from
T22746 49478-49486 NNP denotes American
T22747 49487-49490 NNP denotes Red
T22748 49491-49496 NN denotes cross
T22749 49497-49500 CC denotes and
T22750 49501-49505 VBP denotes have
T22751 49506-49510 VBN denotes been
T22752 49511-49519 VBN denotes approved
T22753 49520-49522 IN denotes by
T22754 49523-49526 DT denotes the
T22755 49527-49531 NNP denotes Ohio
T22756 49532-49537 NNP denotes State
T22757 49538-49548 NNP denotes University
T22758 49549-49555 NN denotes review
T22759 49556-49561 NN denotes board
T22760 49562-49563 -LRB- denotes (
T22761 49563-49566 NNP denotes ARC
T22762 49567-49570 NNP denotes IRB
T22763 49571-49579 NNP denotes Protocol
T22764 49580-49581 # denotes #
T22765 49581-49588 CD denotes 2001-26
T22766 49588-49589 -RRB- denotes )
T22767 49589-49590 . denotes .
R100 T132 T129 pobj macrophages,in
R1000 T1372 T1374 nsubjpass complex,sequestered
R1001 T1373 T1374 auxpass is,sequestered
R1002 T1374 T1374 ROOT sequestered,sequestered
R1003 T1375 T1374 prep in,sequestered
R1004 T1376 T1377 det the,cytosol
R1005 T1377 T1375 pobj cytosol,in
R1006 T1378 T1374 agent by,sequestered
R1007 T1379 T1380 compound IκBα,[
R1008 T1380 T1378 pobj [,by
R1009 T1381 T1382 nummod 15,]
R101 T133 T134 punct (,MDMs
R1010 T1382 T1380 appos ],[
R1011 T1383 T1374 punct .,sequestered
R1012 T1384 T1394 mark Upon,phosphorylated
R1013 T1385 T1384 pobj stimulation,Upon
R1014 T1386 T1385 prep by,stimulation
R1015 T1387 T1386 pobj cytokines,by
R1016 T1388 T1387 cc or,cytokines
R1017 T1389 T1390 compound UV,radiation
R1018 T1390 T1387 conj radiation,cytokines
R1019 T1391 T1390 punct ",",radiation
R102 T134 T132 appos MDMs,macrophages
R1020 T1392 T1393 nsubj IκBα,is
R1021 T1393 T1394 auxpass is,phosphorylated
R1022 T1394 T1394 ROOT phosphorylated,phosphorylated
R1023 T1395 T1394 punct ",",phosphorylated
R1024 T1396 T1394 conj ubiquitinated,phosphorylated
R1025 T1397 T1396 punct ",",ubiquitinated
R1026 T1398 T1396 cc and,ubiquitinated
R1027 T1399 T1396 conj degraded,ubiquitinated
R1028 T1400 T1394 punct ",",phosphorylated
R1029 T1401 T1394 advcl releasing,phosphorylated
R103 T135 T125 punct ),stimulated
R1030 T1402 T1403 compound NF-κB,p50/p65
R1031 T1403 T1401 dobj p50/p65,releasing
R1032 T1404 T1405 aux to,translocate
R1033 T1405 T1401 advcl translocate,releasing
R1034 T1406 T1405 prep to,translocate
R1035 T1407 T1408 det the,nucleus
R1036 T1408 T1406 pobj nucleus,to
R1037 T1409 T1408 cc and,nucleus
R1038 T1410 T1412 compound transactivate,genes
R1039 T1411 T1412 compound target,genes
R104 T136 T125 cc and,stimulated
R1040 T1412 T1408 conj genes,nucleus
R1041 T1413 T1394 punct .,phosphorylated
R1042 T1414 T1423 prep After,undergo
R1043 T1415 T1416 poss its,release
R1044 T1416 T1414 pobj release,After
R1045 T1417 T1416 prep from,release
R1046 T1418 T1417 pobj IκBα,from
R1047 T1419 T1418 punct ",",IκBα
R1048 T1420 T1421 compound NF-κB,p65
R1049 T1421 T1423 nsubj p65,undergo
R105 T137 T142 det the,RAW
R1050 T1422 T1423 aux can,undergo
R1051 T1423 T1423 ROOT undergo,undergo
R1052 T1424 T1425 amod post-translational,modification
R1053 T1425 T1423 dobj modification,undergo
R1054 T1426 T1427 aux to,activate
R1055 T1427 T1423 xcomp activate,undergo
R1056 T1428 T1429 compound gene,transcription
R1057 T1429 T1427 dobj transcription,activate
R1058 T1430 T1423 punct .,undergo
R1059 T1431 T1432 det The,role
R106 T138 T141 nmod murine,line
R1060 T1432 T1441 nsubj role,varies
R10602 T14519 T14521 amod NF-κB,"activation. Here, we demonstrate that M-CSF stimulated NF-κB transcriptional activity"
R10603 T14520 T14521 amod transcription factor Nuclear Factor-kappaB (NF-κB) is a key regulator of genes involved in M-CSF-induced mononuclear phagocyte survival and this study focused at identifying the mechanism of NF-κB transcriptional,"activation. Here, we demonstrate that M-CSF stimulated NF-κB transcriptional activity"
R10604 T14521 T14522 nsubj "activation. Here, we demonstrate that M-CSF stimulated NF-κB transcriptional activity","in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is"
R10605 T14522 T14522 ROOT "in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is","in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is"
R10606 T14523 T14522 acomp important,"in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is"
R10607 T14524 T14523 prep in,important
R10608 T14525 T14524 pcomp regulating,in
R10609 T14526 T14527 amod intracellular,signaling
R1061 T1433 T1432 prep of,role
R10610 T14527 T14525 dobj signaling,regulating
R10611 T14528 T14527 punct ",",signaling
R10612 T14529 T14530 compound stress,response
R10613 T14530 T14527 conj response,signaling
R10614 T14531 T14530 punct ",",response
R10615 T14532 T14530 conj proliferation,response
R10616 T14533 T14532 punct ",",proliferation
R10617 T14534 T14532 conj survival,proliferation
R10618 T14535 T14534 punct ",",survival
R10619 T14536 T14534 conj differentiation,survival
R1062 T1434 T1433 pobj NF-κB,of
R10620 T14537 T14536 cc and,differentiation
R10621 T14538 T14536 conj inflammation,differentiation
R10622 T14539 T14536 conj [,differentiation
R10623 T14540 T14541 nummod 8,]
R10624 T14541 T14530 npadvmod ],response
R10625 T14542 T14522 punct .,"in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is"
R10626 T14543 T14544 aux To,regulate
R10627 T14544 T14551 advcl regulate,translocate
R10628 T14545 T14546 compound gene,transcription
R10629 T14546 T14544 dobj transcription,regulate
R1063 T1435 T1436 nummod p65,phosphorylation
R10630 T14547 T14546 punct ",",transcription
R10631 T14548 T14551 npadvmod NF-κB,translocate
R10632 T14549 T14550 nummod p50/p65,heterodimers
R10633 T14550 T14551 nsubj heterodimers,translocate
R10634 T14551 T14563 dep translocate,is
R10635 T14552 T14551 prep to,translocate
R10636 T14553 T14554 det the,nucleus
R10637 T14554 T14552 pobj nucleus,to
R10638 T14555 T14554 prep from,nucleus
R10639 T14556 T14557 det the,cytoplasm
R1064 T1436 T1441 nsubj phosphorylation,varies
R10640 T14557 T14555 pobj cytoplasm,from
R10641 T14558 T14551 punct ",",translocate
R10642 T14559 T14551 advmod however,translocate
R10643 T14560 T14563 punct ",",is
R10644 T14561 T14562 poss their,translocation
R10645 T14562 T14563 nsubj translocation,is
R10646 T14563 T14563 ROOT is,is
R10647 T14564 T14563 neg not,is
R10648 T14565 T14563 acomp sufficient,is
R10649 T14566 T14567 aux to,activate
R1065 T1437 T1436 prep on,phosphorylation
R10650 T14567 T14565 xcomp activate,sufficient
R10651 T14568 T14569 compound gene,transcription
R10652 T14569 T14567 dobj transcription,activate
R10653 T14570 T14563 punct .,is
R10654 T14571 T14572 amod Post-translational,modification
R10655 T14572 T14582 nsubj modification,contributes
R10656 T14573 T14572 prep of,modification
R10657 T14574 T14575 amod NF-κB,heterodimers
R10658 T14575 T14573 pobj heterodimers,of
R10659 T14576 T14577 amod such,as
R1066 T1438 T1440 amod NF-κB,activity
R10660 T14577 T14575 prep as,heterodimers
R10661 T14578 T14577 pobj phosphorylation,as
R10662 T14579 T14578 cc or,phosphorylation
R10663 T14580 T14578 conj acetylation,phosphorylation
R10664 T14581 T14582 advmod also,contributes
R10665 T14582 T14582 ROOT contributes,contributes
R10666 T14583 T14582 prep to,contributes
R10667 T14584 T14586 poss its,activity
R10668 T14585 T14586 amod transcriptional,activity
R10669 T14586 T14583 pobj activity,to
R1067 T1439 T1440 amod transcriptional,activity
R10670 T14587 T14589 nmod [,]
R10671 T14588 T14589 nummod 41,]
R10672 T14589 T14586 appos ],activity
R10673 T14590 T14589 punct ",",]
R10674 T14591 T14593 nmod [,]
R10675 T14592 T14593 nummod 42,]
R10676 T14593 T14589 conj ],]
R10677 T14594 T14593 punct ",",]
R10678 T14595 T14597 det [,]
R10679 T14596 T14597 nummod 43,]
R1068 T1440 T1437 pobj activity,on
R10680 T14597 T14593 appos ],]
R10681 T14598 T14582 punct .,contributes
R10682 T14599 T14626 mark Because,sought
R10683 T14600 T14602 amod NF-κB,translocates
R10684 T14601 T14602 advmod constitutively,translocates
R10685 T14602 T14626 advcl translocates,sought
R10686 T14603 T14602 prep to,translocates
R10687 T14604 T14605 det the,nucleus
R10688 T14605 T14603 pobj nucleus,to
R10689 T14606 T14605 prep in,nucleus
R1069 T1441 T1441 ROOT varies,varies
R10690 T14607 T14608 amod monocytic,lineage
R10691 T14608 T14606 pobj lineage,in
R10692 T14609 T14611 nmod [,]
R10693 T14610 T14611 nummod 13,]
R10694 T14611 T14605 appos ],nucleus
R10695 T14612 T14602 cc and,translocates
R10696 T14613 T14602 conj activates,translocates
R10697 T14614 T14615 compound gene,transcription
R10698 T14615 T14613 dobj transcription,activates
R10699 T14616 T14615 cc and,transcription
R107 T139 T141 compound macrophage,line
R1070 T1442 T1441 prep by,varies
R10700 T14617 T14615 conj survival,transcription
R10701 T14618 T14615 prep in,transcription
R10702 T14619 T14620 amod murine,macrophages
R10703 T14620 T14618 pobj macrophages,in
R10704 T14621 T14623 nmod [,]
R10705 T14622 T14623 nummod 11,]
R10706 T14623 T14613 dobj ],activates
R10707 T14624 T14626 punct ",",sought
R10708 T14625 T14626 nsubj we,sought
R10709 T14626 T14626 ROOT sought,sought
R1071 T1443 T1442 pobj stimulus,by
R10710 T14627 T14628 aux to,determine
R10711 T14628 T14626 xcomp determine,sought
R10712 T14629 T14634 advmod how,activity
R10713 T14630 T14634 amod M-CSF,activity
R10714 T14631 T14634 amod affected,activity
R10715 T14632 T14634 nmod NF-κB,activity
R10716 T14633 T14634 compound transcriptional,activity
R10717 T14634 T14628 dobj activity,determine
R10718 T14635 T14634 prep in,activity
R10719 T14636 T14637 amod human,MDMs
R1072 T1444 T1453 punct ",",]
R10720 T14637 T14635 pobj MDMs,in
R10721 T14638 T14634 cc and,activity
R10722 T14639 T14642 mark whether,regulated
R10723 T14640 T14641 det this,activation
R10724 T14641 T14642 nsubj activation,regulated
R10725 T14642 T14634 conj regulated,activity
R10726 T14643 T14644 poss their,survival
R10727 T14644 T14642 dobj survival,regulated
R10728 T14645 T14626 punct .,sought
R10729 T14646 T14648 det The,study
R1073 T1445 T1453 npadvmod time,]
R10730 T14647 T14648 amod present,study
R10731 T14648 T14649 nsubj study,demonstrated
R10732 T14649 T14649 ROOT demonstrated,demonstrated
R10733 T14650 T14652 mark that,stimulated
R10734 T14651 T14652 nsubj M-CSF,stimulated
R10735 T14652 T14649 ccomp stimulated,demonstrated
R10736 T14653 T14654 compound PKCα,kinase
R10737 T14654 T14655 compound kinase,upstream
R10738 T14655 T14652 dobj upstream,stimulated
R10739 T14656 T14655 prep of,upstream
R1074 T1446 T1445 prep of,time
R10740 T14657 T14659 amod NF-κB,activity
R10741 T14658 T14659 amod transcriptional,activity
R10742 T14659 T14656 pobj activity,of
R10743 T14660 T14659 prep in,activity
R10744 T14661 T14663 amod primary,MDMs
R10745 T14662 T14663 amod human,MDMs
R10746 T14663 T14660 pobj MDMs,in
R10747 T14664 T14663 cc and,MDMs
R10748 T14665 T14663 conj RAW,MDMs
R10749 T14666 T14667 nummod 264.7,cells
R1075 T1447 T1446 pobj stimulation,of
R10750 T14667 T14659 appos cells,activity
R10751 T14668 T14649 punct .,demonstrated
R10752 T14669 T14670 nsubj We,showed
R10753 T14670 T14685 ccomp showed,blocked
R10754 T14671 T14670 dobj inhibition,showed
R10755 T14672 T14671 prep of,inhibition
R10756 T14673 T14674 amod conventional,PKCs
R10757 T14674 T14672 pobj PKCs,of
R10758 T14675 T14670 prep by,showed
R10759 T14676 T14677 compound PKC,inhibitors
R1076 T1448 T1447 cc and,stimulation
R10760 T14677 T14675 pobj inhibitors,by
R10761 T14678 T14677 punct ",",inhibitors
R10762 T14679 T14685 intj kinase,blocked
R10763 T14680 T14685 advmod deficient,blocked
R10764 T14681 T14685 nsubj PKCα,blocked
R10765 T14682 T14681 cc or,PKCα
R10766 T14683 T14684 compound PKCα,siRNA
R10767 T14684 T14681 conj siRNA,PKCα
R10768 T14685 T14685 ROOT blocked,blocked
R10769 T14686 T14688 amod M-CSF-induced,survival
R1077 T1449 T1450 compound cell,type
R10770 T14687 T14688 compound cell,survival
R10771 T14688 T14685 dobj survival,blocked
R10772 T14689 T14688 cc and,survival
R10773 T14690 T14692 amod NF-κB-regulated,expression
R10774 T14691 T14692 compound gene,expression
R10775 T14692 T14688 conj expression,survival
R10776 T14693 T14685 punct .,blocked
R10777 T14694 T14695 det This,block
R10778 T14695 T14695 ROOT block,block
R10779 T14696 T14695 acl correlated,block
R1078 T1450 T1447 conj type,stimulation
R10780 T14697 T14696 prep with,correlated
R10781 T14698 T14699 det a,reduction
R10782 T14699 T14697 pobj reduction,with
R10783 T14700 T14699 prep in,reduction
R10784 T14701 T14702 amod M-CSF-induced,phosphorylation
R10785 T14702 T14700 pobj phosphorylation,in
R10786 T14703 T14702 prep of,phosphorylation
R10787 T14704 T14705 compound NF-κB,p65
R10788 T14705 T14703 pobj p65,of
R10789 T14706 T14699 prep at,reduction
R1079 T1451 T1453 nmod [,]
R10790 T14707 T14706 pobj Ser276,at
R10791 T14708 T14695 punct .,block
R10792 T14709 T14719 advmod Consistent,inhibited
R10793 T14710 T14709 prep with,Consistent
R10794 T14711 T14712 det these,findings
R10795 T14712 T14710 pobj findings,with
R10796 T14713 T14717 det the,constructs
R10797 T14714 T14717 amod dominant,constructs
R10798 T14715 T14717 amod negative,constructs
R10799 T14716 T14717 compound PKCα,constructs
R108 T140 T141 compound cell,line
R1080 T1452 T1453 nummod 16,]
R10800 T14717 T14719 nsubj constructs,inhibited
R10801 T14718 T14719 advmod also,inhibited
R10802 T14719 T14719 ROOT inhibited,inhibited
R10803 T14720 T14722 nsubj NF-κB,phosphorylation
R10804 T14721 T14722 nummod p65,phosphorylation
R10805 T14722 T14719 dobj phosphorylation,inhibited
R10806 T14723 T14722 prep at,phosphorylation
R10807 T14724 T14723 pobj Ser276,at
R10808 T14725 T14723 cc but,at
R10809 T14726 T14729 neg not,resulting
R1081 T1453 T1441 npadvmod ],varies
R10810 T14727 T14726 prep at,not
R10811 T14728 T14727 pobj Ser536,at
R10812 T14729 T14719 advcl resulting,inhibited
R10813 T14730 T14729 prep in,resulting
R10814 T14731 T14734 amod reduced,activation
R10815 T14732 T14734 nmod NF-κB,activation
R10816 T14733 T14734 amod transcriptional,activation
R10817 T14734 T14730 pobj activation,in
R10818 T14735 T14734 cc and,activation
R10819 T14736 T14737 compound M-CSF-induced,MDM
R1082 T1454 T1441 punct .,varies
R10820 T14737 T14738 compound MDM,survival
R10821 T14738 T14734 conj survival,activation
R10822 T14739 T14719 punct .,inhibited
R10823 T14740 T14744 det A,model
R10824 T14741 T14744 amod simplified,model
R10825 T14742 T14744 amod proposed,model
R10826 T14743 T14744 compound cartoon,model
R10827 T14744 T14748 nsubj model,demonstrates
R10828 T14745 T14744 prep in,model
R10829 T14746 T14745 pobj Figure,in
R1083 T1455 T1456 amod Previous,research
R10830 T14747 T14746 nummod 9,Figure
R10831 T14748 T14748 ROOT demonstrates,demonstrates
R10832 T14749 T14751 mark that,induced
R10833 T14750 T14751 nsubj M-CSF,induced
R10834 T14751 T14748 ccomp induced,demonstrates
R10835 T14752 T14753 compound monocytes,survival
R10836 T14753 T14751 dobj survival,induced
R10837 T14754 T14755 auxpass is,regulated
R10838 T14755 T14751 conj regulated,induced
R10839 T14756 T14755 prep by,regulated
R1084 T1456 T1457 nsubj research,shows
R10840 T14757 T14756 pcomp activating,by
R10841 T14758 T14760 nsubj NF-κB,phosphorylation
R10842 T14759 T14760 nummod p65,phosphorylation
R10843 T14760 T14757 dobj phosphorylation,activating
R10844 T14761 T14760 prep at,phosphorylation
R10845 T14762 T14761 pobj Ser276,at
R10846 T14763 T14757 prep via,activating
R10847 T14764 T14763 pobj PKC,via
R10848 T14765 T14748 punct .,demonstrates
R10849 T14766 T14780 advmod Finally,confirmed
R1085 T1457 T1457 ROOT shows,shows
R10850 T14767 T14780 punct ",",confirmed
R10851 T14768 T14780 prep in,confirmed
R10852 T14769 T14777 det a,line
R10853 T14770 T14777 nmod NF-κB,line
R10854 T14771 T14772 nummod p65,−
R10855 T14772 T14773 nummod −,/
R10856 T14773 T14777 nmod /,line
R10857 T14774 T14777 nmod −,line
R10858 T14775 T14777 compound fibroblast,line
R10859 T14776 T14777 compound cell,line
R1086 T1458 T1469 mark that,plays
R10860 T14777 T14768 pobj line,in
R10861 T14778 T14780 punct ",",confirmed
R10862 T14779 T14780 nsubj we,confirmed
R10863 T14780 T14780 ROOT confirmed,confirmed
R10864 T14781 T14788 mark that,is
R10865 T14782 T14784 det the,residue
R10866 T14783 T14784 compound Ser276,residue
R10867 T14784 T14788 nsubj residue,is
R10868 T14785 T14784 prep of,residue
R10869 T14786 T14785 pobj NF-κB,of
R1087 T1459 T1469 nsubj phosphorylation,plays
R10870 T14787 T14786 nummod p65,NF-κB
R10871 T14788 T14780 ccomp is,confirmed
R10872 T14789 T14788 acomp important,is
R10873 T14790 T14789 cc and,important
R10874 T14791 T14789 conj essential,important
R10875 T14792 T14788 prep for,is
R10876 T14793 T14794 compound PKC,modulation
R10877 T14794 T14792 pobj modulation,for
R10878 T14795 T14794 prep of,modulation
R10879 T14796 T14797 amod NF-κB,activity
R1088 T1460 T1459 prep of,phosphorylation
R10880 T14797 T14795 pobj activity,of
R10881 T14798 T14780 punct .,confirmed
R10882 T14799 T14800 advmod More,compelling
R10883 T14800 T14801 acomp compelling,is
R10884 T14801 T14801 ROOT is,is
R10885 T14802 T14804 mark that,observed
R10886 T14803 T14804 nsubj we,observed
R10887 T14804 T14801 ccomp observed,is
R10888 T14805 T14807 det a,regulation
R10889 T14806 T14807 amod similar,regulation
R1089 T1461 T1462 compound NF-κB,p65
R10890 T14807 T14804 dobj regulation,observed
R10891 T14808 T14807 prep between,regulation
R10892 T14809 T14812 nummod two,lineages
R10893 T14810 T14812 amod different,lineages
R10894 T14811 T14812 compound cell,lineages
R10895 T14812 T14808 pobj lineages,between
R10896 T14813 T14812 prep in,lineages
R10897 T14814 T14815 poss our,study
R10898 T14815 T14813 pobj study,in
R10899 T14816 T14801 punct .,is
R109 T141 T142 compound line,RAW
R1090 T1462 T1460 pobj p65,of
R10900 T14817 T14818 amod Post-translational,modification
R10901 T14818 T14822 nsubj modification,regulates
R10902 T14819 T14818 prep of,modification
R10903 T14820 T14819 pobj NF-κB,of
R10904 T14821 T14820 nummod p65,NF-κB
R10905 T14822 T14822 ROOT regulates,regulates
R10906 T14823 T14824 poss its,activating
R10907 T14824 T14822 dobj activating,regulates
R10908 T14825 T14824 cc or,activating
R10909 T14826 T14824 conj repressing,activating
R1091 T1463 T1459 prep at,phosphorylation
R10910 T14827 T14826 dobj effects,repressing
R10911 T14828 T14827 prep on,effects
R10912 T14829 T14830 compound gene,expression
R10913 T14830 T14828 pobj expression,on
R10914 T14831 T14822 punct .,regulates
R10915 T14832 T14835 prep Among,reported
R10916 T14833 T14834 det the,seven
R10917 T14834 T14835 nsubj seven,reported
R10918 T14835 T14835 ROOT reported,reported
R10919 T14836 T14840 amod putative,sites
R1092 T1464 T1463 pobj Ser276,at
R10920 T14837 T14840 nmod NF-κB,sites
R10921 T14838 T14840 nummod p65,sites
R10922 T14839 T14840 compound phosphorylation,sites
R10923 T14840 T14835 dobj sites,reported
R10924 T14841 T14840 punct ",",sites
R10925 T14842 T14843 nummod five,increase
R10926 T14843 T14840 appos increase,sites
R10927 T14844 T14845 amod nuclear,translocation
R10928 T14845 T14843 appos translocation,increase
R10929 T14846 T14843 punct ",",increase
R1093 T1465 T1464 punct ",",Ser276
R10930 T14847 T14848 nsubj DNA,binding
R10931 T14848 T14852 amod binding,activity
R10932 T14849 T14848 cc and,binding
R10933 T14850 T14848 conj NF-κB,binding
R10934 T14851 T14852 amod transcriptional,activity
R10935 T14852 T14855 nsubj activity,]
R10936 T14853 T14855 nmod [,]
R10937 T14854 T14855 nummod 17,]
R10938 T14855 T14843 appos ],increase
R10939 T14856 T14835 punct .,reported
R1094 T1466 T1464 conj Ser529,Ser276
R10940 T14857 T14865 advmod Similar,found
R10941 T14858 T14857 prep to,Similar
R10942 T14859 T14860 poss our,finding
R10943 T14860 T14858 pobj finding,to
R10944 T14861 T14865 punct ",",found
R10945 T14862 T14864 compound Zhong,al.
R10946 T14863 T14864 compound et,al.
R10947 T14864 T14865 nsubj al.,found
R10948 T14865 T14865 ROOT found,found
R10949 T14866 T14874 mark that,is
R1095 T1467 T1466 cc or,Ser529
R10950 T14867 T14874 prep in,is
R10951 T14868 T14867 pobj response,in
R10952 T14869 T14868 prep to,response
R10953 T14870 T14869 pobj LPS,to
R10954 T14871 T14870 punct ",",LPS
R10955 T14872 T14873 compound NF-κB,p65
R10956 T14873 T14874 nsubj p65,is
R10957 T14874 T14865 ccomp is,found
R10958 T14875 T14874 attr phosphorylated,is
R10959 T14876 T14875 prep at,phosphorylated
R1096 T1468 T1466 conj Ser536,Ser529
R10960 T14877 T14881 det the,residue
R10961 T14878 T14879 advmod highly,conserved
R10962 T14879 T14881 amod conserved,residue
R10963 T14880 T14881 compound Ser276,residue
R10964 T14881 T14876 pobj residue,at
R10965 T14882 T14875 agent by,phosphorylated
R10966 T14883 T14885 det the,subunit
R10967 T14884 T14885 amod catalytic,subunit
R10968 T14885 T14882 pobj subunit,by
R10969 T14886 T14885 prep of,subunit
R1097 T1469 T1457 ccomp plays,shows
R10970 T14887 T14888 compound PKA,[
R10971 T14888 T14886 pobj [,of
R10972 T14889 T14890 nummod 16,]
R10973 T14890 T14888 appos ],[
R10974 T14891 T14865 punct .,found
R10975 T14892 T14900 prep In,phosphorylated
R10976 T14893 T14892 pobj addition,In
R10977 T14894 T14893 prep to,addition
R10978 T14895 T14894 pobj PKA,to
R10979 T14896 T14900 punct ",",phosphorylated
R1098 T1470 T1472 det an,role
R10980 T14897 T14900 nsubjpass Ser276,phosphorylated
R10981 T14898 T14900 aux can,phosphorylated
R10982 T14899 T14900 auxpass be,phosphorylated
R10983 T14900 T14900 ROOT phosphorylated,phosphorylated
R10984 T14901 T14900 agent by,phosphorylated
R10985 T14902 T14901 pobj MSK1,by
R10986 T14903 T14900 prep in,phosphorylated
R10987 T14904 T14905 det the,nucleus
R10988 T14905 T14903 pobj nucleus,in
R10989 T14906 T14905 prep upon,nucleus
R1099 T1471 T1472 amod important,role
R10990 T14907 T14908 compound TNFα,treatment
R10991 T14908 T14906 pobj treatment,upon
R10992 T14909 T14911 nmod [,]
R10993 T14910 T14911 nummod 18,]
R10994 T14911 T14908 appos ],treatment
R10995 T14912 T14900 punct .,phosphorylated
R10996 T14913 T14919 nsubj Phosphorylation,occurs
R10997 T14914 T14913 prep of,Phosphorylation
R10998 T14915 T14916 compound NF-κB,p65
R10999 T14916 T14914 pobj p65,of
R110 T142 T125 conj RAW,stimulated
R1100 T1472 T1469 dobj role,plays
R11000 T14917 T14913 prep at,Phosphorylation
R11001 T14918 T14917 pobj Ser536,at
R11002 T14919 T14919 ROOT occurs,occurs
R11003 T14920 T14919 prep in,occurs
R11004 T14921 T14920 pobj response,in
R11005 T14922 T14921 prep to,response
R11006 T14923 T14925 amod many,stimuli
R11007 T14924 T14925 amod inflammatory,stimuli
R11008 T14925 T14922 pobj stimuli,to
R11009 T14926 T14928 advmod as,as
R1101 T1473 T1472 prep in,role
R11010 T14927 T14928 advmod well,as
R11011 T14928 T14925 cc as,stimuli
R11012 T14929 T14925 conj kinases,stimuli
R11013 T14930 T14929 punct ",",kinases
R11014 T14931 T14929 conj IKKα,kinases
R11015 T14932 T14931 punct ",",IKKα
R11016 T14933 T14931 conj IKKβ,IKKα
R11017 T14934 T14933 cc and,IKKβ
R11018 T14935 T14933 conj RSK1,IKKβ
R11019 T14936 T14933 conj [,IKKβ
R1102 T1474 T1473 pcomp regulating,in
R11020 T14937 T14938 nummod 17,]
R11021 T14938 T14933 conj ],IKKβ
R11022 T14939 T14938 punct ",",]
R11023 T14940 T14942 nmod [,]
R11024 T14941 T14942 nummod 18,]
R11025 T14942 T14938 conj ],]
R11026 T14943 T14942 punct ",",]
R11027 T14944 T14946 nmod [,]
R11028 T14945 T14946 nummod 19,]
R11029 T14946 T14942 conj ],]
R1103 T1475 T1476 amod transcriptional,activity
R11030 T14947 T14946 punct ",",]
R11031 T14948 T14946 conj [,]
R11032 T14949 T14950 nummod 20,]
R11033 T14950 T14946 conj ],]
R11034 T14951 T14950 punct ",",]
R11035 T14952 T14950 conj [,]
R11036 T14953 T14954 nummod 21,]
R11037 T14954 T14950 conj ],]
R11038 T14955 T14919 punct .,occurs
R11039 T14956 T14968 advmod Notably,revealed
R1104 T1476 T1474 dobj activity,regulating
R11040 T14957 T14968 punct ",",revealed
R11041 T14958 T14968 prep in,revealed
R11042 T14959 T14958 pobj addition,in
R11043 T14960 T14959 prep to,addition
R11044 T14961 T14960 pcomp phosphorylating,to
R11045 T14962 T14961 dobj Ser276,phosphorylating
R11046 T14963 T14962 prep of,Ser276
R11047 T14964 T14963 pobj NF-κB,of
R11048 T14965 T14968 punct ",",revealed
R11049 T14966 T14967 poss our,study
R1105 T1477 T1476 prep of,activity
R11050 T14967 T14968 nsubj study,revealed
R11051 T14968 T14968 ROOT revealed,revealed
R11052 T14969 T14972 mark that,modulated
R11053 T14970 T14972 nsubj M-CSF,modulated
R11054 T14971 T14972 advmod also,modulated
R11055 T14972 T14968 ccomp modulated,revealed
R11056 T14973 T14974 det the,phosphorylation
R11057 T14974 T14972 dobj phosphorylation,modulated
R11058 T14975 T14974 prep of,phosphorylation
R11059 T14976 T14979 det the,subunit
R1106 T1478 T1479 compound NF-κB,[
R11060 T14977 T14979 nmod NF-κB,subunit
R11061 T14978 T14979 nummod p65,subunit
R11062 T14979 T14975 pobj subunit,of
R11063 T14980 T14979 prep at,subunit
R11064 T14981 T14980 pobj Ser536,at
R11065 T14982 T14972 punct ",",modulated
R11066 T14983 T14972 advmod however,modulated
R11067 T14984 T14972 punct ",",modulated
R11068 T14985 T14986 compound PKC,inhibitors
R11069 T14986 T14972 dobj inhibitors,modulated
R1107 T1479 T1477 pobj [,of
R11070 T14987 T14972 cc or,modulated
R11071 T14988 T14972 conj kinase,modulated
R11072 T14989 T14991 amod deficient,constructs
R11073 T14990 T14991 compound PKCα,constructs
R11074 T14991 T14988 dobj constructs,kinase
R11075 T14992 T14994 aux did,affect
R11076 T14993 T14994 neg not,affect
R11077 T14994 T14972 conj affect,modulated
R11078 T14995 T14997 det this,event
R11079 T14996 T14997 amod phosphorylation,event
R1108 T1480 T1481 nummod 17,]
R11080 T14997 T14994 dobj event,affect
R11081 T14998 T14968 punct .,revealed
R11082 T14999 T15014 advmod Moreover,reduce
R11083 T15000 T15014 punct ",",reduce
R11084 T15001 T15002 amod mutant,constructs
R11085 T15002 T15014 nsubj constructs,reduce
R11086 T15003 T15002 acl containing,constructs
R11087 T15004 T15006 det a,mutation
R11088 T15005 T15006 compound point,mutation
R11089 T15006 T15003 dobj mutation,containing
R1109 T1481 T1474 dobj ],regulating
R11090 T15007 T15006 prep at,mutation
R11091 T15008 T15007 pobj Ser536,at
R11092 T15009 T15008 prep of,Ser536
R11093 T15010 T15009 pobj NF-κB,of
R11094 T15011 T15010 nummod p65,NF-κB
R11095 T15012 T15014 aux did,reduce
R11096 T15013 T15014 neg not,reduce
R11097 T15014 T15014 ROOT reduce,reduce
R11098 T15015 T15016 amod NF-κB,activity
R11099 T15016 T15014 dobj activity,reduce
R111 T143 T142 nummod 264.7,RAW
R1110 T1482 T1457 punct .,shows
R11100 T15017 T15014 prep in,reduce
R11101 T15018 T15017 pobj cells,in
R11102 T15019 T15018 acl lacking,cells
R11103 T15020 T15022 amod endogenous,p65
R11104 T15021 T15022 compound NF-κB,p65
R11105 T15022 T15019 dobj p65,lacking
R11106 T15023 T15014 punct ",",reduce
R11107 T15024 T15029 mark while,construct
R11108 T15025 T15028 det the,276S/A
R11109 T15026 T15028 nmod NF-κB,276S/A
R1111 T1483 T1493 prep In,phosphorylated
R11110 T15027 T15028 nummod p65,276S/A
R11111 T15028 T15029 nsubj 276S/A,construct
R11112 T15029 T15014 advcl construct,reduce
R11113 T15030 T15031 aux did,reduce
R11114 T15031 T15014 conj reduce,reduce
R11115 T15032 T15033 compound NF-κB,activity
R11116 T15033 T15031 dobj activity,reduce
R11117 T15034 T15031 prep in,reduce
R11118 T15035 T15036 det these,cells
R11119 T15036 T15034 pobj cells,in
R1112 T1484 T1486 amod TNFα-treated,fibroblasts
R11120 T15037 T15014 punct .,reduce
R11121 T15038 T15046 advmod Therefore,is
R11122 T15039 T15046 punct ",",is
R11123 T15040 T15042 amod specific,modification
R11124 T15041 T15042 amod post-translational,modification
R11125 T15042 T15046 nsubj modification,is
R11126 T15043 T15042 prep of,modification
R11127 T15044 T15043 pobj NF-κB,of
R11128 T15045 T15044 nummod p65,NF-κB
R11129 T15046 T15046 ROOT is,is
R1113 T1485 T1486 compound murine,fibroblasts
R11130 T15047 T15046 acomp likely,is
R11131 T15048 T15049 aux to,regulate
R11132 T15049 T15047 xcomp regulate,likely
R11133 T15050 T15051 amod transcriptional,activation
R11134 T15051 T15049 dobj activation,regulate
R11135 T15052 T15049 prep in,regulate
R11136 T15053 T15052 pobj response,in
R11137 T15054 T15053 prep to,response
R11138 T15055 T15056 amod specific,stimuli
R11139 T15056 T15054 pobj stimuli,to
R1114 T1486 T1483 pobj fibroblasts,In
R11140 T15057 T15059 nmod [,]
R11141 T15058 T15059 nummod 15,]
R11142 T15059 T15056 appos ],stimuli
R11143 T15060 T15059 punct ",",]
R11144 T15061 T15063 nmod [,]
R11145 T15062 T15063 nummod 43,]
R11146 T15063 T15059 appos ],]
R11147 T15064 T15046 punct .,is
R11148 T15065 T15066 nsubj It,is
R11149 T15066 T15066 ROOT is,is
R1115 T1487 T1493 punct ",",phosphorylated
R11150 T15067 T15066 acomp notable,is
R11151 T15068 T15070 mark that,activated
R11152 T15069 T15070 nsubj M-CSF,activated
R11153 T15070 T15066 ccomp activated,is
R11154 T15071 T15070 dobj NF-κB,activated
R11155 T15072 T15074 nummod p65,phosphorylation
R11156 T15073 T15074 compound Ser276,phosphorylation
R11157 T15074 T15071 appos phosphorylation,NF-κB
R11158 T15075 T15074 cc and,phosphorylation
R11159 T15076 T15077 amod transcriptional,activation
R1116 T1488 T1493 nsubjpass Ser276,phosphorylated
R11160 T15077 T15074 conj activation,phosphorylation
R11161 T15078 T15077 prep in,activation
R11162 T15079 T15081 det a,manner
R11163 T15080 T15081 amod PKCα-dependent,manner
R11164 T15081 T15078 pobj manner,in
R11165 T15082 T15066 punct .,is
R11166 T15083 T15084 nsubj PKCα,is
R11167 T15084 T15084 ROOT is,is
R11168 T15085 T15086 det a,member
R11169 T15086 T15084 attr member,is
R1117 T1489 T1488 prep of,Ser276
R11170 T15087 T15086 prep of,member
R11171 T15088 T15091 det the,family
R11172 T15089 T15091 amod conventional,family
R11173 T15090 T15091 compound PKC,family
R11174 T15091 T15087 pobj family,of
R11175 T15092 T15091 prep of,family
R11176 T15093 T15094 compound protein,kinases
R11177 T15094 T15092 pobj kinases,of
R11178 T15095 T15096 nsubj that,are
R11179 T15096 T15094 relcl are,kinases
R1118 T1490 T1489 pobj NF-κB,of
R11180 T15097 T15096 acomp critical,are
R11181 T15098 T15097 prep for,critical
R11182 T15099 T15100 compound cell,growth
R11183 T15100 T15098 pobj growth,for
R11184 T15101 T15100 punct ",",growth
R11185 T15102 T15100 conj differentiation,growth
R11186 T15103 T15102 cc and,differentiation
R11187 T15104 T15105 compound cell,death
R11188 T15105 T15102 conj death,differentiation
R11189 T15106 T15084 punct .,is
R1119 T1491 T1490 nummod p65,NF-κB
R11190 T15107 T15109 nsubj PKCα,modulates
R11191 T15108 T15109 advmod primarily,modulates
R11192 T15109 T15109 ROOT modulates,modulates
R11193 T15110 T15109 dobj anti-apoptotic,modulates
R11194 T15111 T15110 cc and,anti-apoptotic
R11195 T15112 T15110 conj proliferation,anti-apoptotic
R11196 T15113 T15109 conj signals,modulates
R11197 T15114 T15113 acl following,signals
R11198 T15115 T15116 amod cytokine,treatment
R11199 T15116 T15114 dobj treatment,following
R112 T144 T122 punct .,demonstrate
R1120 T1492 T1493 auxpass is,phosphorylated
R11200 T15117 T15116 prep in,treatment
R11201 T15118 T15120 amod various,types
R11202 T15119 T15120 compound cell,types
R11203 T15120 T15117 pobj types,in
R11204 T15121 T15123 nmod [,]
R11205 T15122 T15123 nummod 42,]
R11206 T15123 T15116 nmod ],treatment
R11207 T15124 T15109 punct .,modulates
R11208 T15125 T15128 nsubj Expression,attenuates
R11209 T15126 T15125 prep of,Expression
R1121 T1493 T1493 ROOT phosphorylated,phosphorylated
R11210 T15127 T15126 pobj PKCα,of
R11211 T15128 T15128 ROOT attenuates,attenuates
R11212 T15129 T15128 dobj apoptosis,attenuates
R11213 T15130 T15128 prep in,attenuates
R11214 T15131 T15134 amod many,types
R11215 T15132 T15134 amod different,types
R11216 T15133 T15134 compound cell,types
R11217 T15134 T15130 pobj types,in
R11218 T15135 T15128 punct ",",attenuates
R11219 T15136 T15140 mark while,potentiates
R1122 T1494 T1493 agent by,phosphorylated
R11220 T15137 T15138 compound PKCα,inhibition
R11221 T15138 T15140 nsubj inhibition,potentiates
R11222 T15139 T15140 advmod generally,potentiates
R11223 T15140 T15128 advcl potentiates,attenuates
R11224 T15141 T15142 compound apoptosis,[
R11225 T15142 T15140 dobj [,potentiates
R11226 T15143 T15144 nummod 42,]
R11227 T15144 T15142 appos ],[
R11228 T15145 T15128 punct .,attenuates
R11229 T15146 T15160 prep In,was
R1123 T1495 T1494 pobj MSK1,by
R11230 T15147 T15148 poss our,studies
R11231 T15148 T15146 pobj studies,In
R11232 T15149 T15148 acl using,studies
R11233 T15150 T15152 amod conventional,inhibitors
R11234 T15151 T15152 compound PKC,inhibitors
R11235 T15152 T15149 dobj inhibitors,using
R11236 T15153 T15152 cc and,inhibitors
R11237 T15154 T15157 amod dominant,constructs
R11238 T15155 T15157 amod negative,constructs
R11239 T15156 T15157 compound PKCα,constructs
R1124 T1496 T1497 aux to,enhance
R11240 T15157 T15152 conj constructs,inhibitors
R11241 T15158 T15160 punct ",",was
R11242 T15159 T15160 nsubj PKCα,was
R11243 T15160 T15160 ROOT was,was
R11244 T15161 T15160 acomp critical,was
R11245 T15162 T15161 prep in,critical
R11246 T15163 T15165 amod M-CSF-induced,survival
R11247 T15164 T15165 compound macrophage,survival
R11248 T15165 T15162 pobj survival,in
R11249 T15166 T15160 prep by,was
R1125 T1497 T1493 advcl enhance,phosphorylated
R11250 T15167 T15166 pcomp inducing,by
R11251 T15168 T15169 compound Ser276,phosphorylation
R11252 T15169 T15167 dobj phosphorylation,inducing
R11253 T15170 T15169 prep of,phosphorylation
R11254 T15171 T15170 pobj NF-κB,of
R11255 T15172 T15171 nummod p65,NF-κB
R11256 T15173 T15169 prep in,phosphorylation
R11257 T15174 T15177 preconj both,MDMs
R11258 T15175 T15177 amod primary,MDMs
R11259 T15176 T15177 amod human,MDMs
R1126 T1498 T1500 amod NF-κB,activity
R11260 T15177 T15173 pobj MDMs,in
R11261 T15178 T15177 cc and,MDMs
R11262 T15179 T15177 conj Raw,MDMs
R11263 T15180 T15181 nummod 264.7,cells
R11264 T15181 T15177 conj cells,MDMs
R11265 T15182 T15160 punct .,was
R11266 T15183 T15185 nsubj We,demonstrated
R11267 T15184 T15185 advmod also,demonstrated
R11268 T15185 T15185 ROOT demonstrated,demonstrated
R11269 T15186 T15187 compound PKC,inhibition
R1127 T1499 T1500 amod transcriptional,activity
R11270 T15187 T15188 nsubj inhibition,downregulated
R11271 T15188 T15185 ccomp downregulated,demonstrated
R11272 T15189 T15188 dobj NF-κB-induction,downregulated
R11273 T15190 T15189 prep of,NF-κB-induction
R11274 T15191 T15193 det the,gene
R11275 T15192 T15193 compound anti-apoptotic,gene
R11276 T15193 T15194 compound gene,BCL-xL
R11277 T15194 T15190 pobj BCL-xL,of
R11278 T15195 T15185 punct .,demonstrated
R11279 T15196 T15202 advcl Consistent,reported
R1128 T1500 T1497 dobj activity,enhance
R11280 T15197 T15196 prep with,Consistent
R11281 T15198 T15199 poss our,finding
R11282 T15199 T15197 pobj finding,with
R11283 T15200 T15202 punct ",",reported
R11284 T15201 T15202 nsubj others,reported
R11285 T15202 T15202 ROOT reported,reported
R11286 T15203 T15204 nsubj NF-κB,regulated
R11287 T15204 T15202 ccomp regulated,reported
R11288 T15205 T15207 det the,expression
R11289 T15206 T15207 compound gene,expression
R1129 T1501 T1503 nmod [,]
R11290 T15207 T15204 dobj expression,regulated
R11291 T15208 T15207 prep of,expression
R11292 T15209 T15211 amod many,proteins
R11293 T15210 T15211 amod anti-apoptotic,proteins
R11294 T15211 T15208 pobj proteins,of
R11295 T15212 T15211 prep including,proteins
R11296 T15213 T15216 amod other,members
R11297 T15214 T15216 compound BCL-2,members
R11298 T15215 T15216 compound family,members
R11299 T15216 T15212 pobj members,including
R113 T145 T147 det The,protein
R1130 T1502 T1503 nummod 18,]
R11300 T15217 T15216 cc and,members
R11301 T15218 T15216 conj inhibitor,members
R11302 T15219 T15218 prep of,inhibitor
R11303 T15220 T15224 amod apoptosis,proteins
R11304 T15221 T15222 punct (,IAP
R11305 T15222 T15220 appos IAP,apoptosis
R11306 T15223 T15222 punct ),IAP
R11307 T15224 T15219 pobj proteins,of
R11308 T15225 T15226 nsubj which,are
R11309 T15226 T15224 relcl are,proteins
R1131 T1503 T1500 appos ],activity
R11310 T15227 T15226 attr regulators,are
R11311 T15228 T15227 prep of,regulators
R11312 T15229 T15228 pobj apoptosis,of
R11313 T15230 T15229 cc and,apoptosis
R11314 T15231 T15232 compound function,upstream
R11315 T15232 T15229 conj upstream,apoptosis
R11316 T15233 T15232 prep of,upstream
R11317 T15234 T15235 compound caspases,[
R11318 T15235 T15237 nmod [,]
R11319 T15236 T15237 nummod 44,]
R1132 T1504 T1493 punct .,phosphorylated
R11320 T15237 T15233 pobj ],of
R11321 T15238 T15202 punct .,reported
R11322 T15239 T15242 nsubjpass It,shown
R11323 T15240 T15242 aux has,shown
R11324 T15241 T15242 auxpass been,shown
R11325 T15242 T15242 ROOT shown,shown
R11326 T15243 T15249 mark that,prevents
R11327 T15244 T15249 nsubj phosphorylation,prevents
R11328 T15245 T15244 prep of,phosphorylation
R11329 T15246 T15245 pobj p65,of
R1133 T1505 T1515 prep In,associated
R11330 T15247 T15244 prep at,phosphorylation
R11331 T15248 T15247 pobj Ser276,at
R11332 T15249 T15242 ccomp prevents,shown
R11333 T15250 T15251 poss its,degradation
R11334 T15251 T15249 dobj degradation,prevents
R11335 T15252 T15251 prep by,degradation
R11336 T15253 T15254 amod ubiquitin-mediated,proteolysis
R11337 T15254 T15252 pobj proteolysis,by
R11338 T15255 T15249 cc and,prevents
R11339 T15256 T15249 conj promotes,prevents
R1134 T1506 T1505 pobj macrophages,In
R11340 T15257 T15258 compound cell,survival
R11341 T15258 T15256 dobj survival,promotes
R11342 T15259 T15256 prep in,promotes
R11343 T15260 T15261 compound HeLa,cells
R11344 T15261 T15259 pobj cells,in
R11345 T15262 T15264 nmod [,]
R11346 T15263 T15264 nummod 24,]
R11347 T15264 T15261 appos ],cells
R11348 T15265 T15242 punct .,shown
R11349 T15266 T15279 prep In,evaluating
R1135 T1507 T1506 acl treated,macrophages
R11350 T15267 T15266 pobj addition,In
R11351 T15268 T15267 prep to,addition
R11352 T15269 T15270 amod BCL-xL,expression
R11353 T15270 T15268 pobj expression,to
R11354 T15271 T15272 mark as,examined
R11355 T15272 T15279 advcl examined,evaluating
R11356 T15273 T15272 prep in,examined
R11357 T15274 T15275 det this,study
R11358 T15275 T15273 pobj study,in
R11359 T15276 T15279 punct ",",evaluating
R1136 T1508 T1507 prep with,treated
R11360 T15277 T15279 nsubj we,evaluating
R11361 T15278 T15279 aux are,evaluating
R11362 T15279 T15279 ROOT evaluating,evaluating
R11363 T15280 T15281 det the,possibility
R11364 T15281 T15279 dobj possibility,evaluating
R11365 T15282 T15286 mark that,upregulates
R11366 T15283 T15285 amod PKCα-regulated,activity
R11367 T15284 T15285 compound NF-κB,activity
R11368 T15285 T15286 nsubj activity,upregulates
R11369 T15286 T15281 acl upregulates,possibility
R1137 T1509 T1508 pobj endotoxin,with
R11370 T15287 T15289 amod additional,genes
R11371 T15288 T15289 amod anti-apoptotic,genes
R11372 T15289 T15286 dobj genes,upregulates
R11373 T15290 T15289 prep in,genes
R11374 T15291 T15293 det an,fashion
R11375 T15292 T15293 amod M-CSF-dependent,fashion
R11376 T15293 T15290 pobj fashion,in
R11377 T15294 T15295 aux to,modulate
R11378 T15295 T15289 relcl modulate,genes
R11379 T15296 T15297 compound cell,survival
R1138 T1510 T1515 punct ",",associated
R11380 T15297 T15295 dobj survival,modulate
R11381 T15298 T15279 punct .,evaluating
R11382 T15299 T15300 det The,use
R11383 T15300 T15310 nsubj use,ruled
R11384 T15301 T15300 prep of,use
R11385 T15302 T15301 pobj inhibitors,of
R11386 T15303 T15302 cc and,inhibitors
R11387 T15304 T15302 conj siRNA,inhibitors
R11388 T15305 T15302 prep in,inhibitors
R11389 T15306 T15307 poss our,study
R1139 T1511 T1513 amod NF-κB,activity
R11390 T15307 T15305 pobj study,in
R11391 T15308 T15310 aux has,ruled
R11392 T15309 T15310 neg not,ruled
R11393 T15310 T15310 ROOT ruled,ruled
R11394 T15311 T15310 prt out,ruled
R11395 T15312 T15318 mark that,be
R11396 T15313 T15315 amod other,PKCs
R11397 T15314 T15315 amod conventional,PKCs
R11398 T15315 T15318 nsubj PKCs,be
R11399 T15316 T15318 aux might,be
R114 T146 T147 amod general,protein
R1140 T1512 T1513 compound transcription,activity
R11400 T15317 T15318 advmod also,be
R11401 T15318 T15310 ccomp be,ruled
R11402 T15319 T15318 acomp important,be
R11403 T15320 T15318 prep in,be
R11404 T15321 T15323 amod M-CSF-induced,activation
R11405 T15322 T15323 compound NF-κB,activation
R11406 T15323 T15320 pobj activation,in
R11407 T15324 T15323 cc and,activation
R11408 T15325 T15326 compound macrophage,survival
R11409 T15326 T15323 conj survival,activation
R1141 T1513 T1515 nsubjpass activity,associated
R11410 T15327 T15310 punct .,ruled
R11411 T15328 T15347 prep In,appear
R11412 T15329 T15328 pobj contrast,In
R11413 T15330 T15329 prep to,contrast
R11414 T15331 T15332 det the,actions
R11415 T15332 T15330 pobj actions,to
R11416 T15333 T15332 prep of,actions
R11417 T15334 T15335 compound PKCα,activation
R11418 T15335 T15333 pobj activation,of
R11419 T15336 T15335 prep of,activation
R1142 T1514 T1515 auxpass is,associated
R11420 T15337 T15340 amod other,isoforms
R11421 T15338 T15340 amod conventional,isoforms
R11422 T15339 T15340 compound PKC,isoforms
R11423 T15340 T15336 pobj isoforms,of
R11424 T15341 T15340 punct ",",isoforms
R11425 T15342 T15335 prep like,activation
R11426 T15343 T15345 compound PKCβI,βII
R11427 T15344 T15345 compound /,βII
R11428 T15345 T15342 pobj βII,like
R11429 T15346 T15347 punct ",",appear
R1143 T1515 T1515 ROOT associated,associated
R11430 T15347 T15347 ROOT appear,appear
R11431 T15348 T15347 oprd necessary,appear
R11432 T15349 T15348 prep for,necessary
R11433 T15350 T15351 amod macrophage,apoptosis
R11434 T15351 T15349 pobj apoptosis,for
R11435 T15352 T15347 punct .,appear
R11436 T15353 T15357 det The,isoforms
R11437 T15354 T15356 compound PKCβI,βII
R11438 T15355 T15356 compound /,βII
R11439 T15356 T15357 compound βII,isoforms
R1144 T1516 T1515 prep with,associated
R11440 T15357 T15359 nsubjpass isoforms,expressed
R11441 T15358 T15359 auxpass are,expressed
R11442 T15359 T15359 ROOT expressed,expressed
R11443 T15360 T15359 prep in,expressed
R11444 T15361 T15364 amod apoptotic,cells
R11445 T15362 T15364 nmod U937,cells
R11446 T15363 T15364 amod myelomonocytic,cells
R11447 T15364 T15360 pobj cells,in
R11448 T15365 T15367 nmod [,]
R11449 T15366 T15367 nummod 45,]
R1145 T1517 T1516 pobj phosphorylation,with
R11450 T15367 T15359 npadvmod ],expressed
R11451 T15368 T15359 punct .,expressed
R11452 T15369 T15374 det The,HL-525
R11453 T15370 T15372 amod human,cell
R11454 T15371 T15372 compound myeloid,cell
R11455 T15372 T15373 compound cell,line
R11456 T15373 T15374 compound line,HL-525
R11457 T15374 T15381 nsubj HL-525,is
R11458 T15375 T15376 nsubj which,is
R11459 T15376 T15374 relcl is,HL-525
R1146 T1518 T1517 prep on,phosphorylation
R11460 T15377 T15376 acomp devoid,is
R11461 T15378 T15377 prep of,devoid
R11462 T15379 T15380 compound endogenous,PKCβII
R11463 T15380 T15378 pobj PKCβII,of
R11464 T15381 T15381 ROOT is,is
R11465 T15382 T15381 acomp resistant,is
R11466 T15383 T15382 prep to,resistant
R11467 T15384 T15385 amod TNFα-induced,apoptosis
R11468 T15385 T15383 pobj apoptosis,to
R11469 T15386 T15383 pobj [,to
R1147 T1519 T1518 pobj Ser276,on
R11470 T15387 T15388 nummod 46,]
R11471 T15388 T15386 dobj ],[
R11472 T15389 T15381 punct .,is
R11473 T15390 T15396 nsubj Re-expression,restores
R11474 T15391 T15390 prep of,Re-expression
R11475 T15392 T15391 pobj PKCβII,of
R11476 T15393 T15392 prep in,PKCβII
R11477 T15394 T15395 compound HL-525,cells
R11478 T15395 T15393 pobj cells,in
R11479 T15396 T15396 ROOT restores,restores
R1148 T1520 T1519 cc and,Ser276
R11480 T15397 T15398 poss their,susceptibility
R11481 T15398 T15396 dobj susceptibility,restores
R11482 T15399 T15400 aux to,TNFα-induced
R11483 T15400 T15396 xcomp TNFα-induced,restores
R11484 T15401 T15400 dobj apoptosis,TNFα-induced
R11485 T15402 T15400 advcl implying,TNFα-induced
R11486 T15403 T15405 mark that,is
R11487 T15404 T15405 nsubj PKCβII,is
R11488 T15405 T15402 ccomp is,implying
R11489 T15406 T15405 acomp pro-apoptotic,is
R1149 T1521 T1519 conj Ser536,Ser276
R11490 T15407 T15405 cc and,is
R11491 T15408 T15410 aux may,required
R11492 T15409 T15410 auxpass be,required
R11493 T15410 T15405 conj required,is
R11494 T15411 T15412 aux to,induce
R11495 T15412 T15410 advcl induce,required
R11496 T15413 T15414 compound macrophage,apoptosis
R11497 T15414 T15412 dobj apoptosis,induce
R11498 T15415 T15396 punct .,restores
R11499 T15416 T15417 amod Recent,studies
R115 T147 T173 nsubj protein,reduced
R1150 T1522 T1524 nsubjpass that,regulated
R11500 T15417 T15418 nsubj studies,demonstrated
R11501 T15418 T15418 ROOT demonstrated,demonstrated
R11502 T15419 T15425 mark that,regulated
R11503 T15420 T15422 amod NF-κB,activity
R11504 T15421 T15422 amod transcriptional,activity
R11505 T15422 T15425 nsubjpass activity,regulated
R11506 T15423 T15425 aux can,regulated
R11507 T15424 T15425 auxpass be,regulated
R11508 T15425 T15418 ccomp regulated,demonstrated
R11509 T15426 T15425 agent by,regulated
R1151 T1523 T1524 auxpass is,regulated
R11510 T15427 T15426 pobj phosphorylation,by
R11511 T15428 T15427 prep of,phosphorylation
R11512 T15429 T15432 det the,subunit
R11513 T15430 T15432 nmod NF-κB,subunit
R11514 T15431 T15432 nummod p65,subunit
R11515 T15432 T15428 pobj subunit,of
R11516 T15433 T15425 prep in,regulated
R11517 T15434 T15435 det the,absence
R11518 T15435 T15433 pobj absence,in
R11519 T15436 T15435 prep of,absence
R1152 T1524 T1515 conj regulated,associated
R11520 T15437 T15438 compound IκBα,degradation
R11521 T15438 T15436 pobj degradation,of
R11522 T15439 T15441 nmod [,]
R11523 T15440 T15441 nummod 47,]
R11524 T15441 T15438 appos ],degradation
R11525 T15442 T15418 punct .,demonstrated
R11526 T15443 T15450 prep By,regulates
R11527 T15444 T15445 det this,pathway
R11528 T15445 T15443 pobj pathway,By
R11529 T15446 T15450 punct ",",regulates
R1153 T1525 T1524 prep through,regulated
R11530 T15447 T15450 nsubj phosphorylation,regulates
R11531 T15448 T15447 prep of,phosphorylation
R11532 T15449 T15448 pobj NF-κB,of
R11533 T15450 T15450 ROOT regulates,regulates
R11534 T15451 T15452 poss its,interaction
R11535 T15452 T15450 dobj interaction,regulates
R11536 T15453 T15452 prep with,interaction
R11537 T15454 T15455 amod other,components
R11538 T15455 T15453 pobj components,with
R11539 T15456 T15455 prep of,components
R1154 T1526 T1525 pobj protein,through
R11540 T15457 T15460 det the,machinery
R11541 T15458 T15460 amod basal,machinery
R11542 T15459 T15460 amod transcriptional,machinery
R11543 T15460 T15456 pobj machinery,of
R11544 T15461 T15450 prep without,regulates
R11545 T15462 T15461 pcomp affecting,without
R11546 T15463 T15464 poss its,capability
R11547 T15464 T15462 dobj capability,affecting
R11548 T15465 T15466 aux to,bind
R11549 T15466 T15462 xcomp bind,affecting
R1155 T1527 T1528 compound kinase,A
R11550 T15467 T15468 compound DNA,[
R11551 T15468 T15466 dobj [,bind
R11552 T15469 T15470 nummod 21,]
R11553 T15470 T15466 dobj ],bind
R11554 T15471 T15450 punct .,regulates
R11555 T15472 T15475 mark Since,bind
R11556 T15473 T15475 nsubj NF-κB,bind
R11557 T15474 T15475 aux can,bind
R11558 T15475 T15489 advcl bind,affect
R11559 T15476 T15477 det the,promoters
R1156 T1528 T1530 det A,PKA
R11560 T15477 T15475 dobj promoters,bind
R11561 T15478 T15477 prep of,promoters
R11562 T15479 T15481 amod numerous,targets
R11563 T15480 T15481 compound gene,targets
R11564 T15481 T15478 pobj targets,of
R11565 T15482 T15481 punct ",",targets
R11566 T15483 T15484 amod post-translational,modification
R11567 T15484 T15477 conj modification,promoters
R11568 T15485 T15484 prep of,modification
R11569 T15486 T15487 compound NF-κB,p65
R1157 T1529 T1530 punct (,PKA
R11570 T15487 T15485 pobj p65,of
R11571 T15488 T15489 aux may,affect
R11572 T15489 T15489 ROOT affect,affect
R11573 T15490 T15491 poss its,interaction
R11574 T15491 T15489 dobj interaction,affect
R11575 T15492 T15491 prep with,interaction
R11576 T15493 T15494 amod other,proteins
R11577 T15494 T15492 pobj proteins,with
R11578 T15495 T15489 punct ",",affect
R11579 T15496 T15489 advcl refining,affect
R1158 T1530 T1524 oprd PKA,regulated
R11580 T15497 T15498 det the,expression
R11581 T15498 T15496 dobj expression,refining
R11582 T15499 T15498 prep of,expression
R11583 T15500 T15501 compound gene,targets
R11584 T15501 T15499 pobj targets,of
R11585 T15502 T15489 punct .,affect
R11586 T15503 T15506 nsubj We,find
R11587 T15504 T15506 aux did,find
R11588 T15505 T15506 neg not,find
R11589 T15506 T15506 ROOT find,find
R1159 T1531 T1530 punct ),PKA
R11590 T15507 T15510 mark that,involved
R11591 T15508 T15510 nsubjpass PKC,involved
R11592 T15509 T15510 auxpass was,involved
R11593 T15510 T15506 ccomp involved,find
R11594 T15511 T15510 prep in,involved
R11595 T15512 T15513 compound IκBα,degradation
R11596 T15513 T15511 pobj degradation,in
R11597 T15514 T15513 cc or,degradation
R11598 T15515 T15516 compound NF-κB,p65
R11599 T15516 T15518 nummod p65,translocation
R116 T148 T149 compound kinase,C
R1160 T1532 T1530 cc and,PKA
R11600 T15517 T15518 amod nuclear,translocation
R11601 T15518 T15513 conj translocation,degradation
R11602 T15519 T15513 acl induced,degradation
R11603 T15520 T15519 agent by,induced
R11604 T15521 T15520 pobj M-CSF,by
R11605 T15522 T15506 punct ",",find
R11606 T15523 T15506 cc but,find
R11607 T15524 T15526 det that,phosphorylation
R11608 T15525 T15526 amod M-CSF-induced,phosphorylation
R11609 T15526 T15534 nsubjpass phosphorylation,reduced
R1161 T1533 T1530 conj IKKβ,PKA
R11610 T15527 T15526 prep of,phosphorylation
R11611 T15528 T15529 compound NF-κB,p65
R11612 T15529 T15527 pobj p65,of
R11613 T15530 T15526 prep at,phosphorylation
R11614 T15531 T15530 pobj Ser276,at
R11615 T15532 T15534 auxpass was,reduced
R11616 T15533 T15534 advmod dramatically,reduced
R11617 T15534 T15506 conj reduced,find
R11618 T15535 T15534 agent by,reduced
R11619 T15536 T15539 det the,inhibitor
R1162 T1534 T1535 compound respectively,[
R11620 T15537 T15539 amod conventional,inhibitor
R11621 T15538 T15539 compound PKC,inhibitor
R11622 T15539 T15535 pobj inhibitor,by
R11623 T15540 T15539 cc and,inhibitor
R11624 T15541 T15539 conj kinase,inhibitor
R11625 T15542 T15534 advcl deficient,reduced
R11626 T15543 T15542 dobj PKCα,deficient
R11627 T15544 T15542 cc and,deficient
R11628 T15545 T15534 agent by,reduced
R11629 T15546 T15545 pobj siRNA,by
R1163 T1535 T1530 conj [,PKA
R11630 T15547 T15534 prep towards,reduced
R11631 T15548 T15547 pobj PKCα,towards
R11632 T15549 T15534 punct .,reduced
R11633 T15550 T15551 amod Current,studies
R11634 T15551 T15552 nsubj studies,are
R11635 T15552 T15552 ROOT are,are
R11636 T15553 T15552 acomp underway,are
R11637 T15554 T15555 aux to,delineate
R11638 T15555 T15553 xcomp delineate,underway
R11639 T15556 T15558 det the,complexes
R1164 T1536 T1535 nummod 16,[
R11640 T15557 T15558 compound protein,complexes
R11641 T15558 T15555 dobj complexes,delineate
R11642 T15559 T15558 acl formed,complexes
R11643 T15560 T15559 agent by,formed
R11644 T15561 T15562 amod activated,NF-κB
R11645 T15562 T15560 pobj NF-κB,by
R11646 T15563 T15562 cc and,NF-κB
R11647 T15564 T15566 amod other,co-activators
R11648 T15565 T15566 amod transcriptional,co-activators
R11649 T15566 T15562 conj co-activators,NF-κB
R1165 T1537 T1535 conj ],[
R11650 T15567 T15559 prep in,formed
R11651 T15568 T15567 pobj response,in
R11652 T15569 T15568 prep to,response
R11653 T15570 T15569 pobj M-CSF,to
R11654 T15571 T15570 cc and,M-CSF
R11655 T15572 T15573 compound PKC,activation
R11656 T15573 T15570 conj activation,M-CSF
R11657 T15574 T15552 punct .,are
R11658 T15575 T15577 advmod Recently,indicated
R11659 T15576 T15577 nsubj studies,indicated
R1166 T1538 T1537 punct ",",]
R11660 T15577 T15577 ROOT indicated,indicated
R11661 T15578 T15583 mark that,activated
R11662 T15579 T15581 det the,pathway
R11663 T15580 T15581 amod non-canonical,pathway
R11664 T15581 T15583 nsubjpass pathway,activated
R11665 T15582 T15583 auxpass was,activated
R11666 T15583 T15577 ccomp activated,indicated
R11667 T15584 T15583 prep during,activated
R11668 T15585 T15586 amod human,monocyte-macrophage
R11669 T15586 T15587 compound monocyte-macrophage,differentiation
R1167 T1539 T1535 conj [,[
R11670 T15587 T15588 compound differentiation,[
R11671 T15588 T15590 compound [,]
R11672 T15589 T15590 nummod 48,]
R11673 T15590 T15584 pobj ],during
R11674 T15591 T15577 punct .,indicated
R11675 T15592 T15603 prep During,is
R11676 T15593 T15594 det this,process
R11677 T15594 T15592 pobj process,During
R11678 T15595 T15596 punct ",",expression
R11679 T15596 T15603 nsubj expression,is
R1168 T1540 T1539 nummod 19,[
R11680 T15597 T15596 prep of,expression
R11681 T15598 T15597 pobj IKKα,of
R11682 T15599 T15596 prep among,expression
R11683 T15600 T15601 amod other,proteins
R11684 T15601 T15599 pobj proteins,among
R11685 T15602 T15603 punct ",",is
R11686 T15603 T15603 ROOT is,is
R11687 T15604 T15603 acomp elevated,is
R11688 T15605 T15603 advcl leading,is
R11689 T15606 T15605 prep to,leading
R1169 T1541 T1535 conj ],[
R11690 T15607 T15609 amod increased,NF-κB
R11691 T15608 T15609 nummod p52,NF-κB
R11692 T15609 T15606 pobj NF-κB,to
R11693 T15610 T15609 punct (,NF-κB
R11694 T15611 T15609 appos relB,NF-κB
R11695 T15612 T15609 punct ),NF-κB
R11696 T15613 T15605 dobj expression,leading
R11697 T15614 T15605 prep through,leading
R11698 T15615 T15617 amod partial,degradation
R11699 T15616 T15617 amod proteolytic,degradation
R117 T149 T147 appos C,protein
R1170 T1542 T1515 punct .,associated
R11700 T15617 T15614 pobj degradation,through
R11701 T15618 T15617 prep of,degradation
R11702 T15619 T15622 det the,protein
R11703 T15620 T15622 nummod p100,protein
R11704 T15621 T15622 compound NF-κB,protein
R11705 T15622 T15618 pobj protein,of
R11706 T15623 T15603 punct .,is
R11707 T15624 T15627 advmod Thus,is
R11708 T15625 T15627 punct ",",is
R11709 T15626 T15627 nsubj it,is
R1171 T1543 T1550 prep In,is
R11710 T15627 T15627 ROOT is,is
R11711 T15628 T15627 acomp possible,is
R11712 T15629 T15631 mark that,regulates
R11713 T15630 T15631 nsubj M-CSF,regulates
R11714 T15631 T15627 ccomp regulates,is
R11715 T15632 T15633 compound monocyte,survival
R11716 T15633 T15631 dobj survival,regulates
R11717 T15634 T15631 prep through,regulates
R11718 T15635 T15641 predet both,pathways
R11719 T15636 T15641 det the,pathways
R1172 T1544 T1546 amod human,cells
R11720 T15637 T15641 amod canonical,pathways
R11721 T15638 T15637 cc and,canonical
R11722 T15639 T15637 conj non-canonical,canonical
R11723 T15640 T15641 compound NF-κB,pathways
R11724 T15641 T15634 pobj pathways,through
R11725 T15642 T15641 punct ",",pathways
R11726 T15643 T15645 nsubj which,be
R11727 T15644 T15645 aux will,be
R11728 T15645 T15641 relcl be,pathways
R11729 T15646 T15647 det a,focus
R1173 T1545 T1546 compound T,cells
R11730 T15647 T15645 attr focus,be
R11731 T15648 T15647 prep in,focus
R11732 T15649 T15650 amod future,research
R11733 T15650 T15648 pobj research,in
R11734 T15651 T15627 punct .,is
R11735 T15652 T15654 nsubj We,showed
R11736 T15653 T15654 advmod previously,showed
R11737 T15654 T15654 ROOT showed,showed
R11738 T15655 T15657 mark that,reduced
R11739 T15656 T15657 nsubj M-CSF,reduced
R1174 T1546 T1543 pobj cells,In
R11740 T15657 T15654 ccomp reduced,showed
R11741 T15658 T15661 amod caspase-3,activity
R11742 T15659 T15658 cc and,caspase-3
R11743 T15660 T15658 conj -9,caspase-3
R11744 T15661 T15657 dobj activity,reduced
R11745 T15662 T15657 cc and,reduced
R11746 T15663 T15657 conj prevented,reduced
R11747 T15664 T15665 compound monocyte,apoptosis
R11748 T15665 T15666 compound apoptosis,[
R11749 T15666 T15666 ROOT [,[
R1175 T1547 T1550 punct ",",is
R11750 T15667 T15668 nummod 3,]
R11751 T15668 T15663 dobj ],prevented
R11752 T15669 T15654 punct .,showed
R11753 T15670 T15671 det This,study
R11754 T15671 T15672 nsubj study,reveals
R11755 T15672 T15672 ROOT reveals,reveals
R11756 T15673 T15687 mark that,blocked
R11757 T15674 T15687 nsubj treatment,blocked
R11758 T15675 T15674 prep of,treatment
R11759 T15676 T15675 pobj monocytes,of
R1176 T1548 T1549 compound NF-κB,p65
R11760 T15677 T15676 prep with,monocytes
R11761 T15678 T15680 amod conventional,inhibitors
R11762 T15679 T15680 compound PKC,inhibitors
R11763 T15680 T15677 pobj inhibitors,with
R11764 T15681 T15680 punct ",",inhibitors
R11765 T15682 T15676 cc or,monocytes
R11766 T15683 T15676 conj overexpression,monocytes
R11767 T15684 T15683 prep of,overexpression
R11768 T15685 T15686 compound kinase-deficient,PKCα
R11769 T15686 T15684 pobj PKCα,of
R1177 T1549 T1550 nsubj p65,is
R11770 T15687 T15672 ccomp blocked,reveals
R11771 T15688 T15690 amod M-CSF-induced,survival
R11772 T15689 T15690 compound cell,survival
R11773 T15690 T15687 dobj survival,blocked
R11774 T15691 T15672 punct .,reveals
R11775 T15692 T15693 poss Our,data
R11776 T15693 T15694 nsubj data,revealed
R11777 T15694 T15694 ROOT revealed,revealed
R11778 T15695 T15698 mark that,are
R11779 T15696 T15697 amod conventional,PKCs
R1178 T1550 T1550 ROOT is,is
R11780 T15697 T15698 nsubj PKCs,are
R11781 T15698 T15694 ccomp are,revealed
R11782 T15699 T15698 attr upstream,are
R11783 T15700 T15699 prep of,upstream
R11784 T15701 T15702 amod NF-κB,activation
R11785 T15702 T15700 pobj activation,of
R11786 T15703 T15698 prep in,are
R11787 T15704 T15703 pobj response,in
R11788 T15705 T15704 prep to,response
R11789 T15706 T15705 pobj M-CSF,to
R1179 T1551 T1552 advmod constitutively,phosphorylated
R11790 T15707 T15698 cc and,are
R11791 T15708 T15698 conj mediate,are
R11792 T15709 T15710 amod post-translational,activation
R11793 T15710 T15708 dobj activation,mediate
R11794 T15711 T15710 prep of,activation
R11795 T15712 T15711 pobj NF-κB,of
R11796 T15713 T15712 nummod p65,NF-κB
R11797 T15714 T15708 prep by,mediate
R11798 T15715 T15714 pcomp phosphorylating,by
R11799 T15716 T15715 dobj Ser276,phosphorylating
R118 T150 T149 punct (,C
R1180 T1552 T1550 acomp phosphorylated,is
R11800 T15717 T15718 aux to,regulate
R11801 T15718 T15715 advcl regulate,phosphorylating
R11802 T15719 T15720 compound gene,transcription
R11803 T15720 T15718 dobj transcription,regulate
R11804 T15721 T15720 cc and,transcription
R11805 T15722 T15723 compound cell,survival
R11806 T15723 T15720 conj survival,transcription
R11807 T15724 T15694 punct .,revealed
R11808 T15725 T15730 advmod Thus,provide
R11809 T15726 T15730 punct ",",provide
R1181 T1553 T1552 prep on,phosphorylated
R11810 T15727 T15728 poss our,observation
R11811 T15728 T15730 nsubj observation,provide
R11812 T15729 T15730 aux may,provide
R11813 T15730 T15730 ROOT provide,provide
R11814 T15731 T15730 dobj insight,provide
R11815 T15732 T15730 prep in,provide
R11816 T15733 T15735 amod potential,targets
R11817 T15734 T15735 amod therapeutic,targets
R11818 T15735 T15732 pobj targets,in
R11819 T15736 T15735 prep for,targets
R1182 T1554 T1553 pobj Ser536,on
R11820 T15737 T15738 amod inflammatory,diseases
R11821 T15738 T15736 pobj diseases,for
R11822 T15739 T15730 punct .,provide
R1183 T1555 T1556 aux to,facilitate
R1184 T1556 T1552 xcomp facilitate,phosphorylated
R1185 T1557 T1560 nmod NF-κB,translocation
R1186 T1558 T1557 nummod p65,NF-κB
R1187 T1559 T1560 amod nuclear,translocation
R1188 T1560 T1556 dobj translocation,facilitate
R1189 T1561 T1560 prep following,translocation
R119 T151 T149 appos PKC,C
R1190 T1562 T1563 amod cellular,stimulation
R1191 T1563 T1561 pobj stimulation,following
R1192 T1564 T1566 nmod [,]
R1193 T1565 T1566 nummod 20,]
R1194 T1566 T1563 appos ],stimulation
R1195 T1567 T1550 punct .,is
R1196 T1568 T1569 amod Accumulating,evidence
R1197 T1569 T1570 nsubj evidence,reveals
R1198 T1570 T1570 ROOT reveals,reveals
R1199 T1571 T1577 mark that,is
R120 T152 T149 punct ),C
R1200 T1572 T1577 nsubj NF-κB,is
R1201 T1573 T1574 nummod p65,phosphorylation
R1202 T1574 T1577 nsubj phosphorylation,is
R1203 T1575 T1574 prep at,phosphorylation
R1204 T1576 T1575 pobj Ser276,at
R1205 T1577 T1570 ccomp is,reveals
R1206 T1578 T1577 acomp crucial,is
R1207 T1579 T1578 prep for,crucial
R1208 T1580 T1582 poss its,activity
R1209 T1581 T1582 amod transcriptional,activity
R121 T153 T154 compound inhibitor,Ro-31-8220
R1210 T1582 T1579 pobj activity,for
R1211 T1583 T1570 punct .,reveals
R1212 T1584 T1596 prep Upon,recruits
R1213 T1585 T1586 amod nuclear,translocation
R1214 T1586 T1584 pobj translocation,Upon
R1215 T1587 T1596 punct ",",recruits
R1216 T1588 T1596 nsubj phosphorylation,recruits
R1217 T1589 T1588 prep of,phosphorylation
R1218 T1590 T1589 pobj Ser276,of
R1219 T1591 T1588 prep on,phosphorylation
R122 T154 T149 appos Ro-31-8220,C
R1220 T1592 T1591 pobj NF-κB,on
R1221 T1593 T1588 dep p65,phosphorylation
R1222 T1594 T1588 prep by,phosphorylation
R1223 T1595 T1594 pobj PKA,by
R1224 T1596 T1596 ROOT recruits,recruits
R1225 T1597 T1599 det the,co-activator
R1226 T1598 T1599 compound transcription,co-activator
R1227 T1599 T1596 dobj co-activator,recruits
R1228 T1600 T1599 punct ",",co-activator
R1229 T1601 T1599 appos p300,co-activator
R123 T155 T149 punct ",",C
R1230 T1602 T1603 aux to,potentiate
R1231 T1603 T1596 advcl potentiate,recruits
R1232 T1604 T1606 amod NF-κB-regulated,transcription
R1233 T1605 T1606 compound gene,transcription
R1234 T1606 T1603 dobj transcription,potentiate
R1235 T1607 T1609 nmod [,]
R1236 T1608 T1609 nummod 21,]
R1237 T1609 T1609 ROOT ],]
R1238 T1610 T1596 punct .,recruits
R1239 T1611 T1615 advmod However,show
R124 T156 T162 det the,Gö-6976
R1240 T1612 T1615 punct ",",show
R1241 T1613 T1614 amod other,studies
R1242 T1614 T1615 nsubj studies,show
R1243 T1615 T1615 ROOT show,show
R1244 T1616 T1618 mark that,inhibits
R1245 T1617 T1618 nsubj PKA,inhibits
R1246 T1618 T1615 ccomp inhibits,show
R1247 T1619 T1621 amod NF-κB-regulated,expression
R1248 T1620 T1621 compound gene,expression
R1249 T1621 T1618 dobj expression,inhibits
R125 T157 T162 amod conventional,Gö-6976
R1250 T1622 T1618 prep by,inhibits
R1251 T1623 T1622 pcomp stabilizing,by
R1252 T1624 T1625 compound IκBα,[
R1253 T1625 T1623 dobj [,stabilizing
R1254 T1626 T1627 nummod 22,]
R1255 T1627 T1623 dobj ],stabilizing
R1256 T1628 T1629 punct ",",[
R1257 T1629 T1627 npadvmod [,]
R1258 T1630 T1631 nummod 23,]
R1259 T1631 T1627 conj ],]
R126 T158 T162 compound PKCα,Gö-6976
R1260 T1632 T1615 punct .,show
R1261 T1633 T1640 advmod Interestingly,phosphorylates
R1262 T1634 T1640 punct ",",phosphorylates
R1263 T1635 T1638 det the,Pim-1
R1264 T1636 T1638 amod serine/threonine,Pim-1
R1265 T1637 T1638 compound kinase,Pim-1
R1266 T1638 T1640 nsubj Pim-1,phosphorylates
R1267 T1639 T1640 advmod directly,phosphorylates
R1268 T1640 T1640 ROOT phosphorylates,phosphorylates
R1269 T1641 T1642 compound NF-κB,p65
R127 T159 T162 compound /,Gö-6976
R1270 T1642 T1640 dobj p65,phosphorylates
R1271 T1643 T1640 prep at,phosphorylates
R1272 T1644 T1643 pobj Ser276,at
R1273 T1645 T1640 prep by,phosphorylates
R1274 T1646 T1645 pcomp stabilizing,by
R1275 T1647 T1648 aux to,prevent
R1276 T1648 T1646 xcomp prevent,stabilizing
R1277 T1649 T1650 amod ubiquitin-proteasome,degradation
R1278 T1650 T1648 dobj degradation,prevent
R1279 T1651 T1653 nmod [,]
R128 T160 T162 compound β,Gö-6976
R1280 T1652 T1653 nummod 24,]
R1281 T1653 T1650 appos ],degradation
R1282 T1654 T1640 punct .,phosphorylates
R1283 T1655 T1658 amod Several,sites
R1284 T1656 T1658 amod other,sites
R1285 T1657 T1658 amod phosphorylation,sites
R1286 T1658 T1661 nsubjpass sites,described
R1287 T1659 T1661 auxpass are,described
R1288 T1660 T1661 advmod also,described
R1289 T1661 T1661 ROOT described,described
R129 T161 T162 compound inhibitor,Gö-6976
R1290 T1662 T1663 aux to,enhance
R1291 T1663 T1661 xcomp enhance,described
R1292 T1664 T1666 amod NF-κB,transactivation
R1293 T1665 T1666 compound gene,transactivation
R1294 T1666 T1663 dobj transactivation,enhance
R1295 T1667 T1669 nmod [,]
R1296 T1668 T1669 nummod 25,]
R1297 T1669 T1666 appos ],transactivation
R1298 T1670 T1661 punct .,described
R1299 T1671 T1674 advmod Here,investigate
R130 T162 T149 appos Gö-6976,C
R1300 T1672 T1674 punct ",",investigate
R1301 T1673 T1674 nsubj we,investigate
R1302 T1674 T1674 ROOT investigate,investigate
R1303 T1675 T1676 det the,role
R1304 T1676 T1674 dobj role,investigate
R1305 T1677 T1676 prep of,role
R1306 T1678 T1677 pobj protein,of
R1307 T1679 T1680 compound kinase,C
R1308 T1680 T1680 ROOT C,C
R1309 T1681 T1680 punct (,C
R131 T163 T162 punct ",",Gö-6976
R1310 T1682 T1680 appos PKC,C
R1311 T1683 T1680 punct ),C
R1312 T1684 T1676 prep in,role
R1313 T1685 T1686 compound M-CSF-stimulated,NF-κB
R1314 T1686 T1687 compound NF-κB,activation
R1315 T1687 T1684 pobj activation,in
R1316 T1688 T1674 punct .,investigate
R1317 T1689 T1690 compound PKC,proteins
R1318 T1690 T1691 nsubj proteins,are
R1319 T1691 T1691 ROOT are,are
R132 T164 T162 appos overexpression,Gö-6976
R1320 T1692 T1693 amod multifunctional,kinases
R1321 T1693 T1691 attr kinases,are
R1322 T1694 T1695 nsubj that,differ
R1323 T1695 T1693 relcl differ,kinases
R1324 T1696 T1695 prep in,differ
R1325 T1697 T1696 pobj structure,in
R1326 T1698 T1697 punct ",",structure
R1327 T1699 T1695 npadvmod function,differ
R1328 T1700 T1699 cc and,function
R1329 T1701 T1699 conj co-factor,function
R133 T165 T164 prep of,overexpression
R1330 T1702 T1705 compound requirement,]
R1331 T1703 T1705 nmod [,]
R1332 T1704 T1705 nummod 26,]
R1333 T1705 T1693 appos ],kinases
R1334 T1706 T1691 punct .,are
R1335 T1707 T1709 nsubjpass PKCs,involved
R1336 T1708 T1709 auxpass are,involved
R1337 T1709 T1709 ROOT involved,involved
R1338 T1710 T1709 prep in,involved
R1339 T1711 T1713 amod diverse,responses
R134 T166 T169 amod dominant,constructs
R1340 T1712 T1713 compound cell,responses
R1341 T1713 T1710 pobj responses,in
R1342 T1714 T1713 punct ",",responses
R1343 T1715 T1713 prep including,responses
R1344 T1716 T1717 compound cell,growth
R1345 T1717 T1715 pobj growth,including
R1346 T1718 T1717 punct ",",growth
R1347 T1719 T1717 conj survival,growth
R1348 T1720 T1719 punct ",",survival
R1349 T1721 T1719 conj differentiation,survival
R135 T167 T169 amod negative,constructs
R1350 T1722 T1721 cc and,differentiation
R1351 T1723 T1721 conj development,differentiation
R1352 T1724 T1726 nmod [,]
R1353 T1725 T1726 nummod 27,]
R1354 T1726 T1717 appos ],growth
R1355 T1727 T1709 punct .,involved
R1356 T1728 T1732 det The,enzymes
R1357 T1729 T1732 nummod 12,enzymes
R1358 T1730 T1731 advmod closely,related
R1359 T1731 T1732 amod related,enzymes
R136 T168 T169 compound PKCα,constructs
R1360 T1732 T1738 nsubjpass enzymes,divided
R1361 T1733 T1732 prep of,enzymes
R1362 T1734 T1736 det the,family
R1363 T1735 T1736 compound PKC,family
R1364 T1736 T1733 pobj family,of
R1365 T1737 T1738 auxpass are,divided
R1366 T1738 T1738 ROOT divided,divided
R1367 T1739 T1738 prep into,divided
R1368 T1740 T1741 nummod three,classes
R1369 T1741 T1739 pobj classes,into
R137 T169 T165 pobj constructs,of
R1370 T1742 T1741 punct :,classes
R1371 T1743 T1741 appos conventional,classes
R1372 T1744 T1747 punct (,α
R1373 T1745 T1747 dep cPKCs,α
R1374 T1746 T1747 punct :,α
R1375 T1747 T1752 nsubj α,require
R1376 T1748 T1749 compound βI,βII
R1377 T1749 T1752 nsubj βII,require
R1378 T1750 T1749 cc and,βII
R1379 T1751 T1749 conj γ,βII
R138 T170 T169 cc and,constructs
R1380 T1752 T1738 advcl require,divided
R1381 T1753 T1754 amod Ca2,+
R1382 T1754 T1752 dobj +,require
R1383 T1755 T1754 cc and,+
R1384 T1756 T1754 conj diacylglycerol,+
R1385 T1757 T1758 punct (,DAG
R1386 T1758 T1756 appos DAG,diacylglycerol
R1387 T1759 T1756 punct ),diacylglycerol
R1388 T1760 T1752 punct ;,require
R1389 T1761 T1752 conj novel,require
R139 T171 T172 compound PKCα,siRNA
R1390 T1762 T1765 punct (,δ
R1391 T1763 T1765 dep nPKCs,δ
R1392 T1764 T1765 punct :,δ
R1393 T1765 T1761 appos δ,novel
R13934 T18733 T18736 compound Materials,M-CSF
R13935 T18734 T18736 compound Recombinant,M-CSF
R13936 T18735 T18736 compound human,M-CSF
R13937 T18736 T18738 nsubjpass M-CSF,purchased
R13938 T18737 T18738 auxpass was,purchased
R13939 T18738 T18738 ROOT purchased,purchased
R1394 T1766 T1765 punct ",",δ
R13940 T18739 T18738 prep from,purchased
R13941 T18740 T18741 compound R&D,Systems
R13942 T18741 T18739 pobj Systems,from
R13943 T18742 T18741 punct (,Systems
R13944 T18743 T18741 appos Minneapolis,Systems
R13945 T18744 T18743 punct ",",Minneapolis
R13946 T18745 T18743 appos MN,Minneapolis
R13947 T18746 T18743 punct ),Minneapolis
R13948 T18747 T18738 punct .,purchased
R13949 T18748 T18757 nsubjpass Ro-31-8220,obtained
R1395 T1767 T1765 dep ε,δ
R13950 T18749 T18748 punct ",",Ro-31-8220
R13951 T18750 T18748 conj Gö-6976,Ro-31-8220
R13952 T18751 T18750 punct ",",Gö-6976
R13953 T18752 T18750 conj Rotterin,Gö-6976
R13954 T18753 T18752 punct ",",Rotterin
R13955 T18754 T18752 cc and,Rotterin
R13956 T18755 T18752 conj BAPTA/AM,Rotterin
R13957 T18756 T18757 auxpass were,obtained
R13958 T18757 T18757 ROOT obtained,obtained
R13959 T18758 T18757 prep from,obtained
R1396 T1768 T1767 punct ",",ε
R13960 T18759 T18758 pobj Calbiochem,from
R13961 T18760 T18759 punct (,Calbiochem
R13962 T18761 T18762 compound San,Diego
R13963 T18762 T18759 appos Diego,Calbiochem
R13964 T18763 T18762 punct ",",Diego
R13965 T18764 T18759 conj CA,Calbiochem
R13966 T18765 T18757 punct ),obtained
R13967 T18766 T18757 punct .,obtained
R13968 T18767 T18769 amod Low,RPMI
R13969 T18768 T18769 compound endotoxin,RPMI
R1397 T1769 T1765 dep η,δ
R13970 T18769 T18772 nsubj RPMI,serum
R13971 T18770 T18769 cc and,RPMI
R13972 T18771 T18769 conj X-vivo,RPMI
R13973 T18772 T18772 ROOT serum,serum
R13974 T18773 T18774 amod free,medium
R13975 T18774 T18776 nsubjpass medium,obtained
R13976 T18775 T18776 auxpass were,obtained
R13977 T18776 T18772 ccomp obtained,serum
R13978 T18777 T18776 prep from,obtained
R13979 T18778 T18780 compound Lonza,Inc
R1398 T1770 T1769 punct ",",η
R13980 T18779 T18780 compound Walkersville,Inc
R13981 T18780 T18777 pobj Inc,from
R13982 T18781 T18780 punct (,Inc
R13983 T18782 T18780 appos Walkersville,Inc
R13984 T18783 T18782 punct ",",Walkersville
R13985 T18784 T18782 appos MD,Walkersville
R13986 T18785 T18782 punct ),Walkersville
R13987 T18786 T18772 punct .,serum
R13988 T18787 T18789 amod Fetal,serum
R13989 T18788 T18789 amod bovine,serum
R1399 T1771 T1769 dep θ,η
R13990 T18789 T18793 nsubj serum,certified
R13991 T18790 T18791 punct (,FBS
R13992 T18791 T18789 appos FBS,serum
R13993 T18792 T18789 punct ",",serum
R13994 T18793 T18793 ROOT certified,certified
R13995 T18794 T18795 compound <,0.06
R13996 T18795 T18799 nummod 0.06,levels
R13997 T18796 T18799 nmod endotoxin,levels
R13998 T18797 T18799 nmod units/ml,levels
R13999 T18798 T18799 compound endotoxin,levels
R140 T172 T173 nsubj siRNA,reduced
R1400 T1772 T1771 cc and,θ
R14000 T18799 T18793 dobj levels,certified
R14001 T18800 T18799 punct ),levels
R14002 T18801 T18802 auxpass was,purchased
R14003 T18802 T18793 conj purchased,certified
R14004 T18803 T18802 prep from,purchased
R14005 T18804 T18805 compound Atlanta,Biological
R14006 T18805 T18803 pobj Biological,from
R14007 T18806 T18805 punct (,Biological
R14008 T18807 T18805 appos Lawrenceville,Biological
R14009 T18808 T18807 punct ",",Lawrenceville
R1401 T1773 T1771 conj μ,θ
R14010 T18809 T18807 conj GA,Lawrenceville
R14011 T18810 T18805 punct ),Biological
R14012 T18811 T18793 punct .,certified
R14013 T18812 T18816 det All,reagents
R14014 T18813 T18816 amod other,reagents
R14015 T18814 T18815 compound cell,culture
R14016 T18815 T18816 compound culture,reagents
R14017 T18816 T18818 nsubjpass reagents,obtained
R14018 T18817 T18818 auxpass were,obtained
R14019 T18818 T18818 ROOT obtained,obtained
R1402 T1774 T1773 punct ),μ
R14020 T18819 T18818 prep through,obtained
R14021 T18820 T18819 pobj Invitrogen,through
R14022 T18821 T18820 punct (,Invitrogen
R14023 T18822 T18820 appos Carlsbad,Invitrogen
R14024 T18823 T18822 punct ",",Carlsbad
R14025 T18824 T18820 conj CA,Invitrogen
R14026 T18825 T18820 punct ),Invitrogen
R14027 T18826 T18818 punct .,obtained
R14028 T18827 T18829 compound PKCα,p65
R14029 T18828 T18829 compound NF-κB,p65
R1403 T1775 T1765 dep require,δ
R14030 T18829 T18851 nsubjpass p65,obtained
R14031 T18830 T18829 punct ",",p65
R14032 T18831 T18832 compound Lamin,B
R14033 T18832 T18829 appos B,p65
R14034 T18833 T18832 punct ",",B
R14035 T18834 T18832 appos β-actin,B
R14036 T18835 T18832 punct ",",B
R14037 T18836 T18832 cc and,B
R14038 T18837 T18832 conj GAPDH,B
R14039 T18838 T18832 appos antibodies,B
R1404 T1776 T1775 dobj DAG,require
R14040 T18839 T18838 punct ",",antibodies
R14041 T18840 T18838 conj anti-mouse,antibodies
R14042 T18841 T18840 cc and,anti-mouse
R14043 T18842 T18840 conj rabbit,anti-mouse
R14044 T18843 T18845 amod IgG-HRP,antibodies
R14045 T18844 T18845 amod conjugated,antibodies
R14046 T18845 T18840 conj antibodies,anti-mouse
R14047 T18846 T18845 punct ",",antibodies
R14048 T18847 T18840 cc and,anti-mouse
R14049 T18848 T18849 compound PKCα,siRNA
R1405 T1777 T1765 punct ;,δ
R14050 T18849 T18832 appos siRNA,B
R14051 T18850 T18851 auxpass were,obtained
R14052 T18851 T18851 ROOT obtained,obtained
R14053 T18852 T18851 prep from,obtained
R14054 T18853 T18855 compound Santa,Biotechnology
R14055 T18854 T18855 compound Cruz,Biotechnology
R14056 T18855 T18852 pobj Biotechnology,from
R14057 T18856 T18855 punct (,Biotechnology
R14058 T18857 T18858 compound Santa,Cruz
R14059 T18858 T18855 appos Cruz,Biotechnology
R1406 T1778 T1765 cc and,δ
R14060 T18859 T18855 punct ",",Biotechnology
R14061 T18860 T18855 appos CA,Biotechnology
R14062 T18861 T18855 punct ),Biotechnology
R14063 T18862 T18851 punct .,obtained
R14064 T18863 T18864 compound Phospho-NFκB,p65
R14065 T18864 T18872 nsubjpass p65,purchased
R14066 T18865 T18864 punct (,p65
R14067 T18866 T18864 appos Ser276,p65
R14068 T18867 T18866 cc or,Ser276
R14069 T18868 T18866 conj Ser536,Ser276
R1407 T1843 T1844 compound TNFα,mRNA
R14070 T18869 T18864 punct ),p65
R14071 T18870 T18864 conj antibodies,p65
R14072 T18871 T18872 auxpass were,purchased
R14073 T18872 T18872 ROOT purchased,purchased
R14074 T18873 T18872 prep from,purchased
R14075 T18874 T18876 compound Cell,Technology
R14076 T18875 T18876 compound Signaling,Technology
R14077 T18876 T18873 pobj Technology,from
R14078 T18877 T18880 punct (,MA
R14079 T18878 T18880 npadvmod Danvers,MA
R1408 T1779 T1765 conj atypical,δ
R14080 T18879 T18880 punct ",",MA
R14081 T18880 T18876 appos MA,Technology
R14082 T18881 T18880 punct ),MA
R14083 T18882 T18872 punct .,purchased
R14084 T18883 T18884 nsubj DNA,constructs
R14085 T18884 T18884 ROOT constructs,constructs
R14086 T18885 T18884 dobj pTAL-SEAP,constructs
R14087 T18886 T18885 cc and,pTAL-SEAP
R14088 T18887 T18885 conj pNF-κB-SEAP,pTAL-SEAP
R14089 T18888 T18889 auxpass were,obtained
R1409 T1780 T1789 punct (,require
R14090 T18889 T18884 conj obtained,constructs
R14091 T18890 T18889 prep from,obtained
R14092 T18891 T18890 pobj Clontech,from
R14093 T18892 T18891 punct (,Clontech
R14094 T18893 T18894 compound Mountian,View
R14095 T18894 T18891 appos View,Clontech
R14096 T18895 T18894 punct ",",View
R14097 T18896 T18894 npadvmod CA,View
R14098 T18897 T18891 punct ),Clontech
R14099 T18898 T18884 punct .,constructs
R141 T173 T173 ROOT reduced,reduced
R1410 T1844 T1841 conj mRNA,iNOS
R14100 T18899 T18900 compound Expression,vectors
R14101 T18900 T18911 nsubjpass vectors,cloned
R14102 T18901 T18900 acl containing,vectors
R14103 T18902 T18903 compound CMV-p65,WT
R14104 T18903 T18901 dobj WT,containing
R14105 T18904 T18903 punct ",",WT
R14106 T18905 T18906 compound CMV-p65,276S/A
R14107 T18906 T18903 conj 276S/A,WT
R14108 T18907 T18906 cc and,276S/A
R14109 T18908 T18909 compound CMV-p65,536S/A
R1411 T1781 T1789 nsubj aPKCs,require
R14110 T18909 T18906 conj 536S/A,276S/A
R14111 T18910 T18911 auxpass were,cloned
R14112 T18911 T18911 ROOT cloned,cloned
R14113 T18912 T18911 prep in,cloned
R14114 T18913 T18915 compound pBa5e,vector
R14115 T18914 T18915 compound Puro,vector
R14116 T18915 T18912 pobj vector,in
R14117 T18916 T18915 cc or,vector
R14118 T18917 T18918 compound pFLAG-CMV-2,vector
R14119 T18918 T18915 conj vector,vector
R1412 T1845 T1838 dobj production,induces
R14120 T18919 T18920 mark as,described
R14121 T18920 T18911 advcl described,cloned
R14122 T18921 T18924 advmod previously,]
R14123 T18922 T18924 nmod [,]
R14124 T18923 T18924 nummod 49,]
R14125 T18924 T18920 dobj ],described
R14126 T18925 T18911 punct .,cloned
R14127 T18926 T18939 nsubj Kinase,were
R14128 T18927 T18939 advmod deficient,were
R14129 T18928 T18935 nmod CMV-PKCα,pCMV-PKCα
R1413 T1846 T1838 prep after,induces
R14130 T18929 T18930 punct (,KD-PKCα
R14131 T18930 T18928 appos KD-PKCα,CMV-PKCα
R14132 T18931 T18930 punct ),KD-PKCα
R14133 T18932 T18928 cc or,CMV-PKCα
R14134 T18933 T18934 amod wild,type
R14135 T18934 T18935 compound type,pCMV-PKCα
R14136 T18935 T18939 nsubj pCMV-PKCα,were
R14137 T18936 T18935 punct (,pCMV-PKCα
R14138 T18937 T18935 appos WT-PKCα,pCMV-PKCα
R14139 T18938 T18935 punct ),pCMV-PKCα
R1414 T1847 T1854 nmod lipopolysaccharide,]
R14140 T18939 T18939 ROOT were,were
R14141 T18940 T18941 amod generous,gifts
R14142 T18941 T18939 attr gifts,were
R14143 T18942 T18941 prep from,gifts
R14144 T18943 T18945 compound Alexandra,Newton
R14145 T18944 T18945 compound C.,Newton
R14146 T18945 T18942 pobj Newton,from
R14147 T18946 T18945 punct (,Newton
R14148 T18947 T18945 appos University,Newton
R14149 T18948 T18947 prep of,University
R1415 T1782 T1783 punct :,ξ
R14150 T18949 T18948 pobj California,of
R14151 T18950 T18945 punct ",",Newton
R14152 T18951 T18952 compound San,Diego
R14153 T18952 T18945 npadvmod Diego,Newton
R14154 T18953 T18952 punct ",",Diego
R14155 T18954 T18952 appos CA,Diego
R14156 T18955 T18945 punct ),Newton
R14157 T18956 T18939 punct .,were
R14158 T18957 T18968 nsubjpass IκBα,obtained
R14159 T18958 T18959 punct (,NFKBIA
R1416 T1848 T1849 punct (,LPS
R14160 T18959 T18957 appos NFKBIA,IκBα
R14161 T18960 T18959 punct ),NFKBIA
R14162 T18961 T18957 cc and,IκBα
R14163 T18962 T18957 conj GAPDH,IκBα
R14164 T18963 T18968 nsubjpass primers,obtained
R14165 T18964 T18963 prep for,primers
R14166 T18965 T18966 amod real-time,RT-PCR
R14167 T18966 T18964 pobj RT-PCR,for
R14168 T18967 T18968 auxpass were,obtained
R14169 T18968 T18968 ROOT obtained,obtained
R1417 T1849 T1847 appos LPS,lipopolysaccharide
R14170 T18969 T18968 prep from,obtained
R14171 T18970 T18969 pobj SABiosciences,from
R14172 T18971 T18970 punct (,SABiosciences
R14173 T18972 T18970 appos Frederick,SABiosciences
R14174 T18973 T18972 punct ",",Frederick
R14175 T18974 T18972 conj MD,Frederick
R14176 T18975 T18972 punct ),Frederick
R14177 T18976 T18968 punct .,obtained
R14178 T18977 T18978 det A,pool
R14179 T18978 T18987 nsubjpass pool,purchased
R1418 T1850 T1849 punct ),LPS
R14180 T18979 T18978 prep of,pool
R14181 T18980 T18979 pobj mouse,of
R14182 T18981 T18980 cc or,mouse
R14183 T18982 T18985 amod human,siRNA
R14184 T18983 T18985 compound PKCα,siRNA
R14185 T18984 T18985 amod specific,siRNA
R14186 T18985 T18978 appos siRNA,pool
R14187 T18986 T18987 auxpass were,purchased
R14188 T18987 T18987 ROOT purchased,purchased
R14189 T18988 T18987 prep from,purchased
R1419 T1851 T1847 conj exposure,lipopolysaccharide
R14190 T18989 T18991 compound Santa,Biotechnology
R14191 T18990 T18991 compound Cruz,Biotechnology
R14192 T18991 T18988 pobj Biotechnology,from
R14193 T18992 T18991 punct (,Biotechnology
R14194 T18993 T18994 compound Santa,cruz
R14195 T18994 T18991 appos cruz,Biotechnology
R14196 T18995 T18991 punct ",",Biotechnology
R14197 T18996 T18991 appos CA,Biotechnology
R14198 T18997 T18987 punct ),purchased
R14199 T18998 T18987 punct .,purchased
R142 T174 T175 amod NF-κB,activity
R1420 T1852 T1854 nmod [,]
R1421 T1783 T1789 nsubj ξ,require
R1422 T1784 T1783 punct ",",ξ
R1423 T1853 T1854 nummod 29,]
R1424 T1785 T1783 dep ι,ξ
R1425 T1854 T1846 pobj ],after
R1426 T1855 T1838 punct .,induces
R1427 T1856 T1861 prep In,suggests
R1428 T1786 T1785 cc and,ι
R1429 T1857 T1856 pobj addition,In
R143 T175 T173 dobj activity,reduced
R1430 T1858 T1861 punct ",",suggests
R1431 T1787 T1785 conj λ,ι
R1432 T1859 T1860 amod accumulating,evidence
R1433 T1860 T1861 nsubj evidence,suggests
R1434 T1861 T1861 ROOT suggests,suggests
R1435 T1788 T1787 punct ),λ
R1436 T1862 T1867 mark that,have
R1437 T1863 T1864 amod conventional,PKCs
R1438 T1864 T1867 nsubj PKCs,have
R1439 T1789 T1779 parataxis require,atypical
R144 T176 T173 prep in,reduced
R1440 T1865 T1864 prep like,PKCs
R1441 T1866 T1865 pobj PKCα,like
R1442 T1867 T1861 ccomp have,suggests
R1443 T1790 T1791 preconj neither,Ca2
R1444 T1868 T1869 amod anti-apoptotic,functions
R1445 T1869 T1867 dobj functions,have
R1446 T1870 T1861 punct .,suggests
R1447 T1791 T1792 intj Ca2,+
R14477 T19447 T19448 amod Cell,Culture
R14478 T19448 T19457 nsubjpass Culture,purchased
R14479 T19449 T19454 det The,RAW
R1448 T1871 T1876 prep For,overexpressed
R14480 T19450 T19453 amod murine,line
R14481 T19451 T19453 compound macrophage,line
R14482 T19452 T19453 compound cell,line
R14483 T19453 T19454 compound line,RAW
R14484 T19454 T19448 appos RAW,Culture
R14485 T19455 T19454 nummod 264.7,RAW
R14486 T19456 T19457 auxpass was,purchased
R14487 T19457 T19457 ROOT purchased,purchased
R14488 T19458 T19457 prep from,purchased
R14489 T19459 T19458 pobj ATCC,from
R1449 T1792 T1789 dobj +,require
R14490 T19460 T19459 punct (,ATCC
R14491 T19461 T19459 appos Manassas,ATCC
R14492 T19462 T19459 punct ",",ATCC
R14493 T19463 T19459 appos VA,ATCC
R14494 T19464 T19459 punct ),ATCC
R14495 T19465 T19457 punct .,purchased
R14496 T19466 T19470 nsubjpass RAW,maintained
R14497 T19467 T19468 nummod 264.7,cells
R14498 T19468 T19470 nsubjpass cells,maintained
R14499 T19469 T19470 auxpass were,maintained
R145 T177 T176 pobj response,in
R1450 T1872 T1871 pobj example,For
R14500 T19470 T19470 ROOT maintained,maintained
R14501 T19471 T19470 prep in,maintained
R14502 T19472 T19471 pobj RPMI,in
R14503 T19473 T19470 conj supplemented,maintained
R14504 T19474 T19473 prep with,supplemented
R14505 T19475 T19476 nummod 5,%
R14506 T19476 T19490 nmod %,sulfate
R14507 T19477 T19490 nmod FBS,sulfate
R14508 T19478 T19477 cc and,FBS
R14509 T19479 T19477 conj antibiotic-antimycotic,FBS
R1451 T1873 T1876 punct ",",overexpressed
R14510 T19480 T19483 punct (,penicillin
R14511 T19481 T19483 nummod 1000,penicillin
R14512 T19482 T19483 compound U/ml,penicillin
R14513 T19483 T19490 nmod penicillin,sulfate
R14514 T19484 T19483 punct ",",penicillin
R14515 T19485 T19486 nummod 1000,µg
R14516 T19486 T19490 compound µg,sulfate
R14517 T19487 T19490 nmod /,sulfate
R14518 T19488 T19490 compound ml,sulfate
R14519 T19489 T19490 compound streptomycin,sulfate
R1452 T1874 T1875 nsubj PKCα,is
R14520 T19490 T19474 pobj sulfate,with
R14521 T19491 T19473 punct ",",supplemented
R14522 T19492 T19473 cc and,supplemented
R14523 T19493 T19496 nummod 250,B
R14524 T19494 T19496 compound ng/ml,B
R14525 T19495 T19496 compound amphotericin,B
R14526 T19496 T19470 npadvmod B,maintained
R14527 T19497 T19496 punct ),B
R14528 T19498 T19470 prep at,maintained
R14529 T19499 T19500 nummod 37,°
R1453 T1875 T1876 auxpass is,overexpressed
R14530 T19500 T19504 nummod °,fibroblasts
R14531 T19501 T19502 compound C.,Mouse
R14532 T19502 T19504 nmod Mouse,fibroblasts
R14533 T19503 T19504 amod embryonic,fibroblasts
R14534 T19504 T19498 pobj fibroblasts,at
R14535 T19505 T19504 punct (,fibroblasts
R14536 T19506 T19504 appos MEFs,fibroblasts
R14537 T19507 T19504 punct ),fibroblasts
R14538 T19508 T19509 compound cell,line
R14539 T19509 T19470 conj line,maintained
R1454 T1876 T1876 ROOT overexpressed,overexpressed
R14540 T19510 T19509 acl lacking,line
R14541 T19511 T19514 amod specific,subunits
R14542 T19512 T19514 nmod NFκB,subunits
R14543 T19513 T19514 nsubj signaling,subunits
R14544 T19514 T19510 dobj subunits,lacking
R14545 T19515 T19516 compound NF-κB,p65
R14546 T19516 T19514 appos p65,subunits
R14547 T19517 T19516 punct (,p65
R14548 T19518 T19519 nummod p65,−
R14549 T19519 T19520 nummod −,/
R1455 T1877 T1876 prep in,overexpressed
R14550 T19520 T19523 nmod /,line
R14551 T19521 T19523 compound −,line
R14552 T19522 T19523 compound cell,line
R14553 T19523 T19516 appos line,p65
R14554 T19524 T19514 punct ),subunits
R14555 T19525 T19527 nmod [,]
R14556 T19526 T19527 nummod 50,]
R14557 T19527 T19529 nsubjpass ],cultured
R14558 T19528 T19529 auxpass were,cultured
R14559 T19529 T19529 ROOT cultured,cultured
R1456 T1793 T1792 cc nor,+
R14560 T19530 T19529 prep in,cultured
R14561 T19531 T19532 compound DMEM,medium
R14562 T19532 T19530 pobj medium,in
R14563 T19533 T19529 conj supplemented,cultured
R14564 T19534 T19533 prep with,supplemented
R14565 T19535 T19536 nummod 10,%
R14566 T19536 T19540 nmod %,solution
R14567 T19537 T19540 nmod FBS,solution
R14568 T19538 T19537 cc and,FBS
R14569 T19539 T19540 amod antibiotic-antimycotic,solution
R1457 T1878 T1879 det a,variety
R14570 T19540 T19534 pobj solution,with
R14571 T19541 T19529 punct .,cultured
R1458 T1879 T1877 pobj variety,in
R1459 T1880 T1879 prep of,variety
R146 T178 T177 prep to,response
R1460 T1881 T1882 compound tumor,cells
R1461 T1794 T1792 conj DAG,+
R1462 T1882 T1880 pobj cells,of
R1463 T1883 T1882 prep including,cells
R1464 T1795 T1789 punct .,require
R1465 T1884 T1883 pobj gliomas,including
R14658 T19666 T19679 nsubjpass Purification,isolated
R14659 T19667 T19666 prep of,Purification
R1466 T1885 T1884 punct ",",gliomas
R14660 T19668 T19669 compound Peripheral,Blood
R14661 T19669 T19670 compound Blood,Monocytes
R14662 T19670 T19667 pobj Monocytes,of
R14663 T19671 T19670 cc and,Monocytes
R14664 T19672 T19673 compound Monocyte-Derived,Macrophages
R14665 T19673 T19670 conj Macrophages,Monocytes
R14666 T19674 T19673 punct (,Macrophages
R14667 T19675 T19673 appos MDMs,Macrophages
R14668 T19676 T19673 punct ),Macrophages
R14669 T19677 T19670 conj Monocytes,Monocytes
R1467 T1886 T1884 conj liver,gliomas
R14670 T19678 T19679 auxpass were,isolated
R14671 T19679 T19679 ROOT isolated,isolated
R14672 T19680 T19679 prep from,isolated
R14673 T19681 T19683 compound source,packs
R14674 T19682 T19683 compound leukocyte,packs
R14675 T19683 T19680 pobj packs,from
R14676 T19684 T19683 acl obtained,packs
R14677 T19685 T19684 prep from,obtained
R14678 T19686 T19689 det the,Cross
R14679 T19687 T19689 compound American,Cross
R1468 T1887 T1886 punct ",",liver
R14680 T19688 T19689 compound Red,Cross
R14681 T19689 T19685 pobj Cross,from
R14682 T19690 T19691 mark as,described
R14683 T19691 T19683 advcl described,packs
R14684 T19692 T19693 advmod previously,[
R14685 T19693 T19695 quantmod [,]
R14686 T19694 T19695 nummod 51,]
R14687 T19695 T19683 appos ],packs
R14688 T19696 T19679 punct .,isolated
R14689 T19697 T19708 nsubjpass Monocytes,purified
R1469 T1888 T1886 cc and,liver
R14690 T19698 T19697 acl used,Monocytes
R14691 T19699 T19698 prep in,used
R14692 T19700 T19701 amod real,time
R14693 T19701 T19703 compound time,experiments
R14694 T19702 T19703 compound PCR,experiments
R14695 T19703 T19699 pobj experiments,in
R14696 T19704 T19703 cc and,experiments
R14697 T19705 T19706 compound transfection,experiments
R14698 T19706 T19703 conj experiments,experiments
R14699 T19707 T19708 auxpass were,purified
R147 T179 T178 pobj M-CSF,to
R1470 T1889 T1886 conj lung,liver
R14700 T19708 T19708 ROOT purified,purified
R14701 T19709 T19708 agent by,purified
R14702 T19710 T19711 amod positive,selection
R14703 T19711 T19709 pobj selection,by
R14704 T19712 T19708 advcl using,purified
R14705 T19713 T19716 compound CD14,Kit
R14706 T19714 T19716 compound Monocyte,Kit
R14707 T19715 T19716 compound Isolation,Kit
R14708 T19716 T19712 dobj Kit,using
R14709 T19717 T19712 prep from,using
R1471 T1890 T1892 nmod [,]
R14710 T19718 T19719 compound Miltenyi,Biotech
R14711 T19719 T19717 pobj Biotech,from
R14712 T19720 T19719 punct (,Biotech
R14713 T19721 T19719 appos Auburn,Biotech
R14714 T19722 T19721 punct ",",Auburn
R14715 T19723 T19721 appos CA,Auburn
R14716 T19724 T19719 punct ),Biotech
R14717 T19725 T19719 punct (,Biotech
R14718 T19726 T19729 advmod >,pure
R14719 T19727 T19728 nummod 90,%
R1472 T1796 T1800 nsubj Monocytes,express
R14720 T19728 T19729 nsubj %,pure
R14721 T19729 T19712 advcl pure,using
R14722 T19730 T19729 punct ),pure
R14723 T19731 T19708 punct .,purified
R14724 T19732 T19738 prep In,isolated
R14725 T19733 T19734 det some,experiments
R14726 T19734 T19732 pobj experiments,In
R14727 T19735 T19738 punct ",",isolated
R14728 T19736 T19738 nsubjpass monocytes,isolated
R14729 T19737 T19738 auxpass were,isolated
R1473 T1891 T1892 nummod 30,]
R14730 T19738 T19738 ROOT isolated,isolated
R14731 T19739 T19738 prep by,isolated
R14732 T19740 T19741 amod clumping,method
R14733 T19741 T19739 pobj method,by
R14734 T19742 T19745 punct (,pure
R14735 T19743 T19744 nummod 70,%
R14736 T19744 T19745 npadvmod %,pure
R14737 T19745 T19741 appos pure,method
R14738 T19746 T19745 punct ),pure
R14739 T19747 T19738 punct .,isolated
R1474 T1892 T1883 pobj ],including
R14740 T19748 T19749 aux To,obtain
R14741 T19749 T19758 advcl obtain,cultured
R14742 T19750 T19751 amod monocyte-derived,macrophages
R14743 T19751 T19749 dobj macrophages,obtain
R14744 T19752 T19751 punct (,macrophages
R14745 T19753 T19751 appos MDMs,macrophages
R14746 T19754 T19751 punct ),macrophages
R14747 T19755 T19758 punct ",",cultured
R14748 T19756 T19758 nsubjpass monocytes,cultured
R14749 T19757 T19758 auxpass were,cultured
R1475 T1893 T1892 punct ",",]
R14750 T19758 T19758 ROOT cultured,cultured
R14751 T19759 T19758 prep in,cultured
R14752 T19760 T19761 compound RPMI-1640,medium
R14753 T19761 T19759 pobj medium,in
R14754 T19762 T19761 acl containing,medium
R14755 T19763 T19764 nummod 10,%
R14756 T19764 T19765 compound %,FBS
R14757 T19765 T19762 dobj FBS,containing
R14758 T19766 T19765 punct ",",FBS
R14759 T19767 T19765 cc and,FBS
R1476 T1797 T1796 cc and,Monocytes
R14760 T19768 T19773 nummod 10,B
R14761 T19769 T19773 compound µg,B
R14762 T19770 T19773 compound /,B
R14763 T19771 T19773 compound ml,B
R14764 T19772 T19773 compound polymyxin,B
R14765 T19773 T19765 conj B,FBS
R14766 T19774 T19773 cc and,B
R14767 T19775 T19777 nummod 20,M-CSF
R14768 T19776 T19777 amod ng/ml,M-CSF
R14769 T19777 T19773 conj M-CSF,B
R1477 T1894 T1896 nmod [,]
R14770 T19778 T19758 punct .,cultured
R1478 T1895 T1896 nummod 31,]
R1479 T1896 T1892 conj ],]
R148 T180 T173 punct .,reduced
R1480 T1798 T1796 conj macrophages,Monocytes
R1481 T1897 T1876 punct .,overexpressed
R1482 T1898 T1905 prep In,induces
R1483 T1799 T1800 advmod predominantly,express
R1484 T1899 T1900 amod epithelial,cells
R1485 T1900 T1898 pobj cells,In
R1486 T1901 T1905 punct ",",induces
R1487 T1800 T1800 ROOT express,express
R14878 T19917 T19927 nmod Electrophoresis,extracts
R14879 T19918 T19917 prep of,Electrophoresis
R1488 T1902 T1905 nsubj inhibition,induces
R14880 T19919 T19922 det the,Analysis
R14881 T19920 T19922 compound Mobility,Analysis
R14882 T19921 T19922 compound Shift,Analysis
R14883 T19922 T19918 pobj Analysis,of
R14884 T19923 T19922 punct (,Analysis
R14885 T19924 T19922 appos EMSA,Analysis
R14886 T19925 T19922 punct ),Analysis
R14887 T19926 T19927 compound Nuclear,extracts
R14888 T19927 T19929 nsubjpass extracts,isolated
R14889 T19928 T19929 auxpass were,isolated
R1489 T1903 T1902 prep of,inhibition
R14890 T19929 T19929 ROOT isolated,isolated
R14891 T19930 T19929 prep from,isolated
R14892 T19931 T19930 pobj MDMs,from
R14893 T19932 T19929 prep by,isolated
R14894 T19933 T19932 pcomp using,by
R14895 T19934 T19937 det a,kit
R14896 T19935 T19936 amod nuclear,extraction
R14897 T19936 T19937 compound extraction,kit
R14898 T19937 T19933 dobj kit,using
R14899 T19938 T19929 prep according,isolated
R149 T181 T184 advmod Interestingly,reduced
R1490 T1801 T1803 amod conventional,isoforms
R14900 T19939 T19938 prep to,according
R14901 T19940 T19941 det the,manufacturer
R14902 T19941 T19943 poss manufacturer,instruction
R14903 T19942 T19941 case 's,manufacturer
R14904 T19943 T19939 pobj instruction,to
R14905 T19944 T19943 prep from,instruction
R14906 T19945 T19946 compound Active,Motif
R14907 T19946 T19944 pobj Motif,from
R14908 T19947 T19951 punct (,)
R14909 T19948 T19951 nmod Carlsbad,)
R1491 T1904 T1903 pobj PKCα,of
R14910 T19949 T19948 punct ",",Carlsbad
R14911 T19950 T19948 conj CA,Carlsbad
R14912 T19951 T19929 punct ),isolated
R14913 T19952 T19929 punct .,isolated
R14914 T19953 T19957 advmod Briefly,lysed
R14915 T19954 T19957 punct ",",lysed
R14916 T19955 T19957 nsubjpass MDMs,lysed
R14917 T19956 T19957 auxpass were,lysed
R14918 T19957 T19957 ROOT lysed,lysed
R14919 T19958 T19957 prep in,lysed
R1492 T1905 T1905 ROOT induces,induces
R14920 T19959 T19961 amod hypotonic,buffer
R14921 T19960 T19961 compound lysis,buffer
R14922 T19961 T19958 pobj buffer,in
R14923 T19962 T19961 punct (,buffer
R14924 T19963 T19965 nummod 20,Hepes
R14925 T19964 T19965 compound mM,Hepes
R14926 T19965 T19961 appos Hepes,buffer
R14927 T19966 T19965 punct ",",Hepes
R14928 T19967 T19965 appos pH,Hepes
R14929 T19968 T19967 nummod 7.5,pH
R1493 T1906 T1907 amod PKCδ-dependent,apoptosis
R14930 T19969 T19965 punct ;,Hepes
R14931 T19970 T19972 nummod 5,NaF
R14932 T19971 T19972 compound mM,NaF
R14933 T19972 T19965 appos NaF,Hepes
R14934 T19973 T19972 punct ",",NaF
R14935 T19974 T19979 nummod 10,NP-40
R14936 T19975 T19976 compound µM,Na2MoO4
R14937 T19976 T19979 compound Na2MoO4,NP-40
R14938 T19977 T19978 nummod 0.5,%
R14939 T19978 T19979 compound %,NP-40
R1494 T1802 T1803 compound PKC,isoforms
R14940 T19979 T19972 appos NP-40,NaF
R14941 T19980 T19979 cc and,NP-40
R14942 T19981 T19983 nummod 0.1,EDTA
R14943 T19982 T19983 compound mM,EDTA
R14944 T19983 T19979 conj EDTA,NP-40
R14945 T19984 T19983 punct ),EDTA
R14946 T19985 T19972 punct ",",NaF
R14947 T19986 T19972 cc and,NaF
R14948 T19987 T19990 advmod then,resuspended
R14949 T19988 T19990 nsubjpass nuclei,resuspended
R1495 T1803 T1800 dobj isoforms,express
R14950 T19989 T19990 auxpass were,resuspended
R14951 T19990 T19957 conj resuspended,lysed
R14952 T19991 T19990 prep in,resuspended
R14953 T19992 T19993 compound lysis,buffer
R14954 T19993 T19991 pobj buffer,in
R14955 T19994 T19990 conj supplemented,resuspended
R14956 T19995 T19994 prep with,supplemented
R14957 T19996 T19998 nummod 0.5,DTT
R14958 T19997 T19998 compound mM,DTT
R14959 T19998 T19995 pobj DTT,with
R1496 T1907 T1908 compound apoptosis,[
R14960 T19999 T19998 cc and,DTT
R14961 T20000 T20002 nummod 0.2,PMSF
R14962 T20001 T20002 compound mM,PMSF
R14963 T20002 T19998 conj PMSF,DTT
R14964 T20003 T19990 punct .,resuspended
R14965 T20004 T20031 det The,incubated
R14966 T20005 T20006 amod NF-κB,consensus
R14967 T20006 T20031 nsubjpass consensus,incubated
R14968 T20007 T20006 appos oligonucleotides,consensus
R14969 T20008 T20007 punct (,oligonucleotides
R1497 T1804 T1803 punct (,isoforms
R14970 T20009 T20006 conj sense,consensus
R14971 T20010 T20006 punct :,consensus
R14972 T20011 T20006 appos AGTTGAGGGGACTTTCCCAGGC,consensus
R14973 T20012 T20006 punct ;,consensus
R14974 T20013 T20017 nsubj antisense,labeled
R14975 T20014 T20013 punct :,antisense
R14976 T20015 T20013 appos GCCTGGGAAAGTCCCCTCAACT,antisense
R14977 T20016 T20013 punct ),antisense
R14978 T20017 T20006 acl labeled,consensus
R14979 T20018 T20017 prep with,labeled
R1498 T1908 T1905 dobj [,induces
R14980 T20019 T20018 pobj 32P,with
R14981 T20020 T20017 agent by,labeled
R14982 T20021 T20022 nummod T4,polynucleokinase
R14983 T20022 T20020 pobj polynucleokinase,by
R14984 T20023 T20024 punct (,Promega
R14985 T20024 T20022 appos Promega,polynucleokinase
R14986 T20025 T20024 punct ",",Promega
R14987 T20026 T20024 conj Madison,Promega
R14988 T20027 T20026 punct ",",Madison
R14989 T20028 T20026 conj WI,Madison
R1499 T1909 T1910 nummod 31,]
R14990 T20029 T20024 punct ),Promega
R14991 T20030 T20031 auxpass were,incubated
R14992 T20031 T20031 ROOT incubated,incubated
R14993 T20032 T20031 prep with,incubated
R14994 T20033 T20034 amod nuclear,extracts
R14995 T20034 T20032 pobj extracts,with
R14996 T20035 T20034 prep in,extracts
R14997 T20036 T20037 amod binding,buffer
R14998 T20037 T20035 pobj buffer,in
R14999 T20038 T20034 punct (,extracts
R150 T182 T184 punct ",",reduced
R1500 T1805 T1806 compound PKCα,PKCβI
R15000 T20039 T20042 nummod 10,pH
R15001 T20040 T20042 compound mM,pH
R15002 T20041 T20042 compound Tris,pH
R15003 T20042 T20034 appos pH,extracts
R15004 T20043 T20047 nummod 7.6,DTT
R15005 T20044 T20047 punct ",",DTT
R15006 T20045 T20047 nummod 1,DTT
R15007 T20046 T20047 compound mM,DTT
R15008 T20047 T20034 appos DTT,extracts
R15009 T20048 T20047 punct ",",DTT
R1501 T1910 T1908 appos ],[
R15010 T20049 T20051 nummod 0.5,EDTA
R15011 T20050 T20051 compound mM,EDTA
R15012 T20051 T20047 conj EDTA,DTT
R15013 T20052 T20051 punct ",",EDTA
R15014 T20053 T20054 nummod 2,µg
R15015 T20054 T20055 compound µg,polydI-dC
R15016 T20055 T20051 appos polydI-dC,EDTA
R15017 T20056 T20055 cc and,polydI-dC
R15018 T20057 T20058 nummod 10,%
R15019 T20058 T20059 compound %,Glycerol
R1502 T1911 T1905 punct .,induces
R15020 T20059 T20055 conj Glycerol,polydI-dC
R15021 T20060 T20059 punct ),Glycerol
R15022 T20061 T20051 prep at,EDTA
R15023 T20062 T20063 nummod 30,°
R15024 T20063 T20061 pobj °,at
R15025 T20064 T20051 conj C,EDTA
R15026 T20065 T20047 prep for,DTT
R15027 T20066 T20067 nummod 30,minutes
R15028 T20067 T20065 pobj minutes,for
R15029 T20068 T20031 punct .,incubated
R1503 T1912 T1927 advmod Interestingly,have
R15030 T20069 T20071 det The,DNA
R15031 T20070 T20071 amod free,DNA
R15032 T20071 T20076 nsubjpass DNA,resolved
R15033 T20072 T20071 cc and,DNA
R15034 T20073 T20074 amod DNA-protein,mixtures
R15035 T20074 T20071 conj mixtures,DNA
R15036 T20075 T20076 auxpass were,resolved
R15037 T20076 T20076 ROOT resolved,resolved
R15038 T20077 T20076 prep in,resolved
R15039 T20078 T20079 nummod 5,%
R1504 T1913 T1927 punct ",",have
R15040 T20079 T20082 nmod %,gels
R15041 T20080 T20082 amod native,gels
R15042 T20081 T20082 compound polyacrylamide,gels
R15043 T20082 T20077 pobj gels,in
R15044 T20083 T20076 prep in,resolved
R15045 T20084 T20087 nummod 0.5,buffer
R15046 T20085 T20087 nummod ×,buffer
R15047 T20086 T20087 compound TBE,buffer
R15048 T20087 T20083 pobj buffer,in
R15049 T20088 T20091 punct (,Tris
R1505 T1806 T1803 appos PKCβI,isoforms
R15050 T20089 T20091 nummod 45,Tris
R15051 T20090 T20091 compound mM,Tris
R15052 T20091 T20087 appos Tris,buffer
R15053 T20092 T20091 punct ",",Tris
R15054 T20093 T20094 nummod 45,mM
R15055 T20094 T20096 nmod mM,acid
R15056 T20095 T20096 compound boric,acid
R15057 T20096 T20091 appos acid,Tris
R15058 T20097 T20096 cc and,acid
R15059 T20098 T20100 nummod 1,EDTA
R1506 T1914 T1927 prep in,have
R15060 T20099 T20100 compound mM,EDTA
R15061 T20100 T20096 conj EDTA,acid
R15062 T20101 T20100 punct ",",EDTA
R15063 T20102 T20100 appos pH,EDTA
R15064 T20103 T20102 nummod 8.3,pH
R15065 T20104 T20100 punct ),EDTA
R15066 T20105 T20076 agent by,resolved
R15067 T20106 T20105 pobj electrophoresis,by
R15068 T20107 T20076 punct .,resolved
R15069 T20108 T20109 det The,gel
R1507 T1915 T1916 amod human,monocytes
R15070 T20109 T20111 nsubjpass gel,dried
R15071 T20110 T20111 auxpass was,dried
R15072 T20111 T20111 ROOT dried,dried
R15073 T20112 T20111 cc and,dried
R15074 T20113 T20111 conj subjected,dried
R15075 T20114 T20115 aux to,autoradiography
R15076 T20115 T20113 xcomp autoradiography,subjected
R15077 T20116 T20115 dobj analysis,autoradiography
R15078 T20117 T20111 punct .,dried
R1508 T1807 T1806 cc and,PKCβI
R1509 T1916 T1914 pobj monocytes,in
R151 T183 T184 nsubj Ro-31-8220,reduced
R1510 T1917 T1916 cc and,monocytes
R1511 T1808 T1806 conj PKCβII,PKCβI
R1512 T1918 T1921 amod premonocytic,cells
R1513 T1919 T1921 compound THP-1,cells
R1514 T1920 T1921 compound leukemia,cells
R1515 T1809 T1806 punct ),PKCβI
R1516 T1921 T1916 conj cells,monocytes
R1517 T1922 T1921 punct ",",cells
R1518 T1923 T1924 compound novel,PKCs
R1519 T1924 T1921 conj PKCs,cells
R152 T184 T184 ROOT reduced,reduced
R1520 T1925 T1924 prep like,PKCs
R1521 T1926 T1925 pobj PKCδ,like
R1522 T1810 T1806 cc and,PKCβI
R1523 T1927 T1938 ccomp have,showed
R1524 T1928 T1930 det the,effect
R1525 T1929 T1930 amod opposite,effect
R1526 T1930 T1927 dobj effect,have
R1527 T1811 T1812 amod novel,PKCs
R15278 T20349 T20350 compound Transient,Transfection
R15279 T20350 T20359 nsubjpass Transfection,seeded
R1528 T1931 T1930 prep on,effect
R15280 T20351 T20350 prep of,Transfection
R15281 T20352 T20351 pobj RAW,of
R15282 T20353 T20355 nummod 264.7,RAW
R15283 T20354 T20355 compound Cells,RAW
R15284 T20355 T20350 appos RAW,Transfection
R15285 T20356 T20357 nummod 264.7,cells
R15286 T20357 T20359 nsubjpass cells,seeded
R15287 T20358 T20359 auxpass were,seeded
R15288 T20359 T20359 ROOT seeded,seeded
R15289 T20360 T20359 prep in,seeded
R1529 T1932 T1933 compound cell,survival
R15290 T20361 T20362 amod 12-well,plates
R15291 T20362 T20360 pobj plates,in
R15292 T20363 T20359 prep in,seeded
R15293 T20364 T20365 compound RPMI,medium
R15294 T20365 T20363 pobj medium,in
R15295 T20366 T20365 acl containing,medium
R15296 T20367 T20368 nummod 5,%
R15297 T20368 T20366 dobj %,containing
R15298 T20369 T20372 nsubj FBS,prior
R15299 T20370 T20371 nummod one,day
R153 T185 T186 compound Ser276,phosphorylation
R1530 T1933 T1931 pobj survival,on
R15300 T20371 T20372 npadvmod day,prior
R15301 T20372 T20368 amod prior,%
R15302 T20373 T20372 prep to,prior
R15303 T20374 T20373 pobj transfection,to
R15304 T20375 T20359 punct ",",seeded
R15305 T20376 T20378 compound Secreted,phosphatase
R15306 T20377 T20378 compound alkaline,phosphatase
R15307 T20378 T20388 nsubjpass phosphatase,transfected
R15308 T20379 T20380 punct (,SEAP
R15309 T20380 T20378 appos SEAP,phosphatase
R1531 T1812 T1806 conj PKCs,PKCβI
R15310 T20381 T20380 punct ),SEAP
R15311 T20382 T20383 compound reporter,plasmids
R15312 T20383 T20384 compound plasmids,pTAL-SEAP
R15313 T20384 T20378 conj pTAL-SEAP,phosphatase
R15314 T20385 T20384 cc or,pTAL-SEAP
R15315 T20386 T20384 conj pNF-κB-SEAP,pTAL-SEAP
R15316 T20387 T20388 auxpass were,transfected
R15317 T20388 T20359 dep transfected,seeded
R15318 T20389 T20388 prep into,transfected
R15319 T20390 T20389 pobj cells,into
R1532 T1934 T1938 punct ",",showed
R15320 T20391 T20390 acl using,cells
R15321 T20392 T20393 compound Qiagene,Effectene
R15322 T20393 T20391 dobj Effectene,using
R15323 T20394 T20393 cc or,Effectene
R15324 T20395 T20397 compound Attractene,kit
R15325 T20396 T20397 compound transfection,kit
R15326 T20397 T20393 conj kit,Effectene
R15327 T20398 T20399 punct (,Qiagen
R15328 T20399 T20397 appos Qiagen,kit
R15329 T20400 T20399 punct ",",Qiagen
R1533 T1935 T1937 compound Voss,al
R15330 T20401 T20399 npadvmod Valencia,Qiagen
R15331 T20402 T20401 punct ",",Valencia
R15332 T20403 T20401 appos CA,Valencia
R15333 T20404 T20399 punct ),Qiagen
R15334 T20405 T20388 prep according,transfected
R15335 T20406 T20405 prep to,according
R15336 T20407 T20408 det the,manufacturer
R15337 T20408 T20410 poss manufacturer,protocol
R15338 T20409 T20408 case 's,manufacturer
R15339 T20410 T20406 pobj protocol,to
R1534 T1813 T1812 punct (,PKCs
R15340 T20411 T20388 punct .,transfected
R15341 T20412 T20421 prep For,were
R15342 T20413 T20414 amod co-transfection,studies
R15343 T20414 T20412 pobj studies,For
R15344 T20415 T20421 punct ",",were
R15345 T20416 T20420 nmod PKCα,constructs
R15346 T20417 T20416 cc or,PKCα
R15347 T20418 T20416 conj NF-κB,PKCα
R15348 T20419 T20418 nummod p65,NF-κB
R15349 T20420 T20421 nsubj constructs,were
R1535 T1936 T1937 compound et,al
R15350 T20421 T20421 ROOT were,were
R15351 T20422 T20421 acomp mixed,were
R15352 T20423 T20422 prep at,mixed
R15353 T20424 T20425 det a,ratio
R15354 T20425 T20423 pobj ratio,at
R15355 T20426 T20425 prep of,ratio
R15356 T20427 T20429 nummod 5,1
R15357 T20428 T20429 nummod ∶,1
R15358 T20429 T20426 pobj 1,of
R15359 T20430 T20425 prep with,ratio
R1536 T1937 T1938 nsubj al,showed
R15360 T20431 T20433 det the,plasmid
R15361 T20432 T20433 compound reporter,plasmid
R15362 T20433 T20430 pobj plasmid,with
R15363 T20434 T20421 punct .,were
R15364 T20435 T20448 prep For,used
R15365 T20436 T20437 amod siRNA,transfection
R15366 T20437 T20435 pobj transfection,For
R15367 T20438 T20448 punct ",",used
R15368 T20439 T20440 nummod 100,nM
R15369 T20440 T20448 nsubjpass nM,used
R1537 T1938 T1938 ROOT showed,showed
R15370 T20441 T20440 prep of,nM
R15371 T20442 T20443 compound PKCα,siRNA
R15372 T20443 T20441 pobj siRNA,of
R15373 T20444 T20440 cc or,nM
R15374 T20445 T20446 compound control,siRNA
R15375 T20446 T20440 conj siRNA,nM
R15376 T20447 T20448 auxpass were,used
R15377 T20448 T20448 ROOT used,used
R15378 T20449 T20448 punct .,used
R15379 T20450 T20452 nsubjpass Cells,incubated
R1538 T1939 T1942 mark that,phosphorylates
R15380 T20451 T20452 auxpass were,incubated
R15381 T20452 T20452 ROOT incubated,incubated
R15382 T20453 T20452 prep with,incubated
R15383 T20454 T20456 det the,reagents
R15384 T20455 T20456 compound transfection,reagents
R15385 T20456 T20453 pobj reagents,with
R15386 T20457 T20452 prep for,incubated
R15387 T20458 T20457 pobj 16,for
R15388 T20459 T20460 nummod 24,hours
R15389 T20460 T20458 appos hours,16
R1539 T1814 T1812 conj PKCδ,PKCs
R15390 T20461 T20452 punct ",",incubated
R15391 T20462 T20452 conj washed,incubated
R15392 T20463 T20462 cc and,washed
R15393 T20464 T20462 conj starved,washed
R15394 T20465 T20464 prep in,starved
R15395 T20466 T20465 pobj RPMI,in
R15396 T20467 T20464 prep without,starved
R15397 T20468 T20467 pobj FBS,without
R15398 T20469 T20464 prep for,starved
R15399 T20470 T20471 nummod 4,hours
R154 T186 T184 dobj phosphorylation,reduced
R1540 T1940 T1942 nsubj PKCδ,phosphorylates
R15400 T20471 T20469 pobj hours,for
R15401 T20472 T20452 punct .,incubated
R15402 T20473 T20476 nsubjpass Samples,stimulated
R15403 T20474 T20476 auxpass were,stimulated
R15404 T20475 T20476 advmod then,stimulated
R15405 T20476 T20476 ROOT stimulated,stimulated
R15406 T20477 T20476 prep with,stimulated
R15407 T20478 T20480 nummod 100,M-CSF
R15408 T20479 T20480 amod ng/ml,M-CSF
R15409 T20480 T20477 pobj M-CSF,with
R1541 T1815 T1814 cc and,PKCδ
R15410 T20481 T20480 prep for,M-CSF
R15411 T20482 T20483 nummod 4,hours
R15412 T20483 T20481 pobj hours,for
R15413 T20484 T20486 preconj at,point
R15414 T20485 T20486 det which,point
R15415 T20486 T20483 relcl point,hours
R15416 T20487 T20488 det the,medium
R15417 T20488 T20490 nsubjpass medium,collected
R15418 T20489 T20490 auxpass was,collected
R15419 T20490 T20476 conj collected,stimulated
R1542 T1816 T1814 conj PKCε,PKCδ
R15420 T20491 T20490 prep for,collected
R15421 T20492 T20494 det the,analysis
R15422 T20493 T20494 compound SEAP,analysis
R15423 T20494 T20491 pobj analysis,for
R15424 T20495 T20490 punct .,collected
R15425 T20496 T20502 prep For,pre-incubated
R15426 T20497 T20498 compound inhibition,studies
R15427 T20498 T20496 pobj studies,For
R15428 T20499 T20502 punct ",",pre-incubated
R15429 T20500 T20502 nsubjpass cells,pre-incubated
R1543 T1817 T1812 punct ),PKCs
R15430 T20501 T20502 auxpass were,pre-incubated
R15431 T20502 T20502 ROOT pre-incubated,pre-incubated
R15432 T20503 T20502 prep with,pre-incubated
R15433 T20504 T20503 pobj inhibitors,with
R15434 T20505 T20504 prep for,inhibitors
R15435 T20506 T20507 nummod 30,minutes
R15436 T20507 T20505 pobj minutes,for
R15437 T20508 T20507 prep before,minutes
R15438 T20509 T20510 compound M-CSF,treatment
R15439 T20510 T20508 pobj treatment,before
R1544 T1818 T1800 punct .,express
R15440 T20511 T20502 punct .,pre-incubated
R15441 T20512 T20516 prep On,realized
R15442 T20513 T20512 amod average,On
R15443 T20514 T20516 punct ",",realized
R15444 T20515 T20516 nsubj we,realized
R15445 T20516 T20516 ROOT realized,realized
R15446 T20517 T20516 dobj 30,realized
R15447 T20518 T20519 nummod 40,%
R15448 T20519 T20521 nmod %,efficiency
R15449 T20520 T20521 compound transfection,efficiency
R1545 T1941 T1942 advmod directly,phosphorylates
R15450 T20521 T20516 conj efficiency,realized
R15451 T20522 T20523 mark as,confirmed
R15452 T20523 T20521 advcl confirmed,efficiency
R15453 T20524 T20523 agent by,confirmed
R15454 T20525 T20526 compound GFP,expression
R15455 T20526 T20524 pobj expression,by
R15456 T20527 T20523 prep in,confirmed
R15457 T20528 T20530 amod Raw,cells
R15458 T20529 T20530 nummod 264.7,cells
R15459 T20530 T20527 pobj cells,in
R1546 T1942 T1938 ccomp phosphorylates,showed
R15460 T20531 T20516 punct .,realized
R1547 T1943 T1942 dobj caspase-3,phosphorylates
R1548 T1819 T1820 amod Conventional,PKCs
R1549 T1944 T1942 cc and,phosphorylates
R155 T187 T186 prep of,phosphorylation
R1550 T1945 T1942 conj promotes,phosphorylates
R1551 T1820 T1821 nsubj PKCs,regulate
R1552 T1946 T1947 amod etoposide-induced,apoptosis
R1553 T1947 T1948 compound apoptosis,[
R1554 T1948 T1945 dobj [,promotes
R1555 T1821 T1821 ROOT regulate,regulate
R1556 T1949 T1950 nummod 32,]
R1557 T1950 T1948 appos ],[
R1558 T1822 T1821 dobj differentiation,regulate
R1559 T1951 T1938 punct .,showed
R156 T188 T189 compound NF-κBp65,leading
R1560 T1952 T1957 advmod Moreover,suggest
R1561 T1823 T1822 prep of,differentiation
R1562 T1953 T1957 punct ",",suggest
R1563 T1954 T1956 compound knockout,studies
R1564 T1955 T1956 compound mouse,studies
R1565 T1824 T1829 det the,line
R15655 T20824 T20825 compound Transient,Transfection
R15656 T20825 T20832 nsubjpass Transfection,transfected
R15657 T20826 T20825 prep of,Transfection
R15658 T20827 T20828 compound Primary,Human
R15659 T20828 T20829 compound Human,Monocytes
R1566 T1956 T1957 nsubj studies,suggest
R15660 T20829 T20830 compound Monocytes,Monocytes
R15661 T20830 T20826 pobj Monocytes,of
R15662 T20831 T20832 auxpass were,transfected
R15663 T20832 T20832 ROOT transfected,transfected
R15664 T20833 T20832 advcl using,transfected
R15665 T20834 T20839 det the,kit
R15666 T20835 T20836 amod human,monocyte
R15667 T20836 T20839 compound monocyte,kit
R15668 T20837 T20839 compound Amaxa,kit
R15669 T20838 T20839 compound nucleofector,kit
R1567 T1957 T1966 advcl suggest,involved
R15670 T20839 T20833 dobj kit,using
R15671 T20840 T20839 punct (,kit
R15672 T20841 T20843 compound Lonza,Inc
R15673 T20842 T20843 compound Walkersville,Inc
R15674 T20843 T20839 appos Inc,kit
R15675 T20844 T20843 punct ",",Inc
R15676 T20845 T20843 conj Walkersville,Inc
R15677 T20846 T20845 punct ",",Walkersville
R15678 T20847 T20845 appos MD,Walkersville
R15679 T20848 T20845 punct ),Walkersville
R1568 T1825 T1828 amod human,cell
R15680 T20849 T20851 mark as,described
R15681 T20850 T20851 advmod previously,described
R15682 T20851 T20833 advcl described,using
R15683 T20852 T20854 nmod [,]
R15684 T20853 T20854 nummod 51,]
R15685 T20854 T20851 dobj ],described
R15686 T20855 T20832 punct .,transfected
R15687 T20856 T20860 advmod Briefly,resuspended
R15688 T20857 T20860 punct ",",resuspended
R15689 T20858 T20860 nsubjpass monocytes,resuspended
R1569 T1958 T1960 mark that,novel
R15690 T20859 T20860 auxpass were,resuspended
R15691 T20860 T20860 ROOT resuspended,resuspended
R15692 T20861 T20860 prep in,resuspended
R15693 T20862 T20863 compound Amaxa,Nucleofactor
R15694 T20863 T20864 compound Nucleofactor,solution
R15695 T20864 T20861 pobj solution,in
R15696 T20865 T20864 prep at,solution
R15697 T20866 T20867 det a,density
R15698 T20867 T20865 pobj density,at
R15699 T20868 T20867 prep of,density
R157 T189 T187 pobj leading,of
R1570 T1959 T1960 det another,novel
R15700 T20869 T20872 nummod 20,cells/ml
R15701 T20870 T20872 nummod ×,cells/ml
R15702 T20871 T20872 nummod 106,cells/ml
R15703 T20872 T20868 pobj cells/ml,of
R15704 T20873 T20860 punct ",",resuspended
R15705 T20874 T20860 cc and,resuspended
R15706 T20875 T20876 nummod 2,µg
R15707 T20876 T20890 nsubjpass µg,added
R15708 T20877 T20876 prep of,µg
R15709 T20878 T20880 amod total,DNA
R1571 T1960 T1957 ccomp novel,suggest
R15710 T20879 T20880 compound plasmid,DNA
R15711 T20880 T20877 pobj DNA,of
R15712 T20881 T20882 nummod 100,nM
R15713 T20882 T20876 conj nM,µg
R15714 T20883 T20882 prep of,nM
R15715 T20884 T20885 compound PKCα,siRNA
R15716 T20885 T20883 pobj siRNA,of
R15717 T20886 T20882 cc or,nM
R15718 T20887 T20888 compound control,siRNA
R15719 T20888 T20882 conj siRNA,nM
R1572 T1961 T1960 appos PKC,novel
R15720 T20889 T20890 auxpass was,added
R15721 T20890 T20860 conj added,resuspended
R15722 T20891 T20890 cc and,added
R15723 T20892 T20890 conj transfected,added
R15724 T20893 T20892 advcl using,transfected
R15725 T20894 T20896 compound program,Y-01
R15726 T20895 T20896 compound Amaxa,Y-01
R15727 T20896 T20893 dobj Y-01,using
R15728 T20897 T20890 punct .,added
R15729 T20898 T20907 prep For,were
R1573 T1962 T1966 punct ",",involved
R15730 T20899 T20900 amod co-transfection,studies
R15731 T20900 T20898 pobj studies,For
R15732 T20901 T20907 punct ",",were
R15733 T20902 T20906 nmod PKCα,constructs
R15734 T20903 T20902 cc or,PKCα
R15735 T20904 T20902 conj NF-κB,PKCα
R15736 T20905 T20904 nummod p65,NF-κB
R15737 T20906 T20907 nsubj constructs,were
R15738 T20907 T20907 ROOT were,were
R15739 T20908 T20907 acomp mixed,were
R1574 T1963 T1966 nsubjpass PKCε,involved
R15740 T20909 T20908 prep at,mixed
R15741 T20910 T20911 det a,ratio
R15742 T20911 T20909 pobj ratio,at
R15743 T20912 T20911 prep of,ratio
R15744 T20913 T20915 nummod 5,1
R15745 T20914 T20915 nummod ∶,1
R15746 T20915 T20912 pobj 1,of
R15747 T20916 T20908 prep with,mixed
R15748 T20917 T20919 det the,plasmid
R15749 T20918 T20919 compound reporter,plasmid
R1575 T1964 T1966 auxpass is,involved
R15750 T20919 T20916 pobj plasmid,with
R15751 T20920 T20907 punct .,were
R15752 T20921 T20922 advmod Immediately,after
R15753 T20922 T20927 prep after,washed
R15754 T20923 T20922 pobj transfection,after
R15755 T20924 T20927 punct ",",washed
R15756 T20925 T20927 nsubjpass cells,washed
R15757 T20926 T20927 auxpass were,washed
R15758 T20927 T20927 ROOT washed,washed
R15759 T20928 T20927 prep with,washed
R1576 T1826 T1828 amod promyelocytic,cell
R15760 T20929 T20930 nummod 1,ml
R15761 T20930 T20928 pobj ml,with
R15762 T20931 T20930 prep of,ml
R15763 T20932 T20933 compound RPMI,medium
R15764 T20933 T20931 pobj medium,of
R15765 T20934 T20930 acl containing,ml
R15766 T20935 T20937 nummod 2,glutamine
R15767 T20936 T20937 compound mM,glutamine
R15768 T20937 T20934 dobj glutamine,containing
R15769 T20938 T20937 cc and,glutamine
R1577 T1965 T1966 advmod critically,involved
R15770 T20939 T20940 nummod 10,%
R15771 T20940 T20941 compound %,FBS
R15772 T20941 T20937 conj FBS,glutamine
R15773 T20942 T20941 cc or,FBS
R15774 T20943 T20944 npadvmod serum,free
R15775 T20944 T20946 amod free,medium
R15776 T20945 T20946 compound LGM,medium
R15777 T20946 T20941 conj medium,FBS
R15778 T20947 T20950 punct (,Inc.
R15779 T20948 T20950 compound Lonza,Inc.
R1578 T1966 T1966 ROOT involved,involved
R15780 T20949 T20950 compound Walkersville,Inc.
R15781 T20950 T20946 appos Inc.,medium
R15782 T20951 T20950 punct ),Inc.
R15783 T20952 T20953 advmod then,plated
R15784 T20953 T20927 dep plated,washed
R15785 T20954 T20953 prep at,plated
R15786 T20955 T20958 nummod 4,cells/well
R15787 T20956 T20958 nummod ×,cells/well
R15788 T20957 T20958 nummod 105,cells/well
R15789 T20958 T20954 pobj cells/well,at
R1579 T1967 T1966 prep in,involved
R15790 T20959 T20958 prep in,cells/well
R15791 T20960 T20961 amod 24-well,plates
R15792 T20961 T20959 pobj plates,in
R15793 T20962 T20927 punct .,washed
R15794 T20963 T20965 det The,medium
R15795 T20964 T20965 amod original,medium
R15796 T20965 T20967 nsubjpass medium,replaced
R15797 T20966 T20967 auxpass was,replaced
R15798 T20967 T20967 ROOT replaced,replaced
R15799 T20968 T20967 agent by,replaced
R158 T190 T189 prep to,leading
R1580 T1827 T1828 compound leukemia,cell
R15800 T20969 T20970 npadvmod serum,free
R15801 T20970 T20972 amod free,medium
R15802 T20971 T20972 compound LGM,medium
R15803 T20972 T20968 pobj medium,by
R15804 T20973 T20967 prep without,replaced
R15805 T20974 T20973 cc or,without
R15806 T20975 T20973 conj with,without
R15807 T20976 T20975 pobj M-CSF,with
R15808 T20977 T20976 punct (,M-CSF
R15809 T20978 T20979 nummod 100,ng/ml
R1581 T1968 T1970 amod early,signaling
R15810 T20979 T20976 appos ng/ml,M-CSF
R15811 T20980 T20979 punct ),ng/ml
R15812 T20981 T20982 nummod one,hour
R15813 T20982 T20983 npadvmod hour,later
R15814 T20983 T20967 advmod later,replaced
R15815 T20984 T20967 punct .,replaced
R15816 T20985 T20987 det The,day
R15817 T20986 T20987 amod next,day
R15818 T20987 T20992 npadvmod day,collected
R15819 T20988 T20992 punct ",",collected
R1582 T1969 T1970 amod LPS-mediated,signaling
R15820 T20989 T20990 amod cell-free,supernatants
R15821 T20990 T20992 nsubjpass supernatants,collected
R15822 T20991 T20992 auxpass were,collected
R15823 T20992 T20992 ROOT collected,collected
R15824 T20993 T20992 prep for,collected
R15825 T20994 T20996 det the,analysis
R15826 T20995 T20996 compound SEAP,analysis
R15827 T20996 T20993 pobj analysis,for
R15828 T20997 T20992 punct .,collected
R15829 T20998 T21000 nsubjpass Cells,stained
R1583 T1970 T1967 pobj signaling,in
R15830 T20999 T21000 auxpass were,stained
R15831 T21000 T21000 ROOT stained,stained
R15832 T21001 T21000 prep with,stained
R15833 T21002 T21004 compound Annexin,iodide
R15834 T21003 T21004 compound V/propidium,iodide
R15835 T21004 T21001 pobj iodide,with
R15836 T21005 T21006 punct (,PI
R15837 T21006 T21004 appos PI,iodide
R15838 T21007 T21004 punct ),iodide
R15839 T21008 T21000 punct .,stained
R1584 T1828 T1829 compound cell,line
R15840 T21009 T21016 prep For,pre-incubated
R15841 T21010 T21012 det the,studies
R15842 T21011 T21012 compound inhibition,studies
R15843 T21012 T21009 pobj studies,For
R15844 T21013 T21016 punct ",",pre-incubated
R15845 T21014 T21016 nsubjpass cells,pre-incubated
R15846 T21015 T21016 auxpass were,pre-incubated
R15847 T21016 T21016 ROOT pre-incubated,pre-incubated
R15848 T21017 T21016 prep with,pre-incubated
R15849 T21018 T21017 pobj inhibitor,with
R1585 T1971 T1970 prep in,signaling
R15850 T21019 T21016 prep for,pre-incubated
R15851 T21020 T21021 nummod 30,minutes
R15852 T21021 T21019 pobj minutes,for
R15853 T21022 T21021 prep in,minutes
R15854 T21023 T21024 compound X-vivo,medium
R15855 T21024 T21022 pobj medium,in
R15856 T21025 T21016 advmod prior,pre-incubated
R15857 T21026 T21025 prep to,prior
R15858 T21027 T21028 det the,addition
R15859 T21028 T21026 pobj addition,to
R1586 T1972 T1973 amod activated,macrophages
R15860 T21029 T21028 prep of,addition
R15861 T21030 T21029 pobj M-CSF,of
R15862 T21031 T21016 punct .,pre-incubated
R1587 T1973 T1971 pobj macrophages,in
R1588 T1829 T1823 pobj line,of
R1589 T1974 T1976 nmod [,]
R159 T191 T194 amod decreased,survival
R1590 T1975 T1976 nummod 33,]
R1591 T1976 T1970 appos ],signaling
R1592 T1830 T1835 nummod HL60,]
R1593 T1977 T1966 punct .,involved
R1594 T1978 T1981 advmod Previously,reported
R1595 T1979 T1981 punct ",",reported
R1596 T1980 T1981 nsubj we,reported
R1597 T1831 T1835 prep to,]
R1598 T1981 T1981 ROOT reported,reported
R1599 T1982 T1984 mark that,promotes
R160 T192 T194 amod M-CSF-induced,survival
R1600 T1832 T1831 pobj macrophages,to
R1601 T1983 T1984 nsubj M-CSF,promotes
R1602 T1984 T1981 ccomp promotes,reported
R1603 T1833 T1835 nmod [,]
R1604 T1985 T1986 compound monocyte,survival
R1605 T1834 T1835 nummod 28,]
R1606 T1986 T1984 dobj survival,promotes
R16061 T21302 T21304 compound Secreted,Phosphatase
R16062 T21303 T21304 compound Alkaline,Phosphatase
R16063 T21304 T21314 nsubjpass Phosphatase,analyzed
R16064 T21305 T21306 punct (,SEAP
R16065 T21306 T21304 appos SEAP,Phosphatase
R16066 T21307 T21306 punct ),SEAP
R16067 T21308 T21309 nsubj Analysis,Secreted
R16068 T21309 T21312 compound Secreted,activity
R16069 T21310 T21312 amod alkaline,activity
R1607 T1835 T1829 appos ],line
R16070 T21311 T21312 compound phosphatase,activity
R16071 T21312 T21314 nsubjpass activity,analyzed
R16072 T21313 T21314 auxpass was,analyzed
R16073 T21314 T21314 ROOT analyzed,analyzed
R16074 T21315 T21314 agent by,analyzed
R16075 T21316 T21317 compound GreatEscape,SEAP
R16076 T21317 T21318 compound SEAP,kit
R16077 T21318 T21315 pobj kit,by
R16078 T21319 T21314 prep according,analyzed
R16079 T21320 T21319 prep to,according
R1608 T1987 T1984 prep through,promotes
R16080 T21321 T21322 det the,manufacturer
R16081 T21322 T21324 poss manufacturer,instruction
R16082 T21323 T21322 case 's,manufacturer
R16083 T21324 T21320 pobj instruction,to
R16084 T21325 T21314 punct .,analyzed
R16085 T21326 T21330 advmod Briefly,diluted
R16086 T21327 T21330 punct ",",diluted
R16087 T21328 T21330 nsubjpass medium,diluted
R16088 T21329 T21330 auxpass was,diluted
R16089 T21330 T21330 ROOT diluted,diluted
R1609 T1988 T1989 det the,activation
R16090 T21331 T21330 prep in,diluted
R16091 T21332 T21334 det the,buffer
R16092 T21333 T21334 compound dilution,buffer
R16093 T21334 T21331 pobj buffer,in
R16094 T21335 T21330 cc and,diluted
R16095 T21336 T21330 conj heated,diluted
R16096 T21337 T21336 prep to,heated
R16097 T21338 T21339 compound 65,°
R16098 T21339 T21340 nummod °,C
R16099 T21340 T21337 pobj C,to
R161 T193 T194 compound monocyte,survival
R1610 T1989 T1987 pobj activation,through
R16100 T21341 T21342 aux to,inactivate
R16101 T21342 T21336 advcl inactivate,heated
R16102 T21343 T21344 amod endogenous,phosphatases
R16103 T21344 T21342 dobj phosphatases,inactivate
R16104 T21345 T21346 advmod then,incubated
R16105 T21346 T21330 conj incubated,diluted
R16106 T21347 T21346 prep with,incubated
R16107 T21348 T21349 det the,substrate
R16108 T21349 T21347 pobj substrate,with
R16109 T21350 T21349 prep for,substrate
R1611 T1836 T1821 punct .,regulate
R16110 T21351 T21352 nummod 30,minutes
R16111 T21352 T21350 pobj minutes,for
R16112 T21353 T21330 punct .,diluted
R16113 T21354 T21356 det The,signal
R16114 T21355 T21356 compound chemiluminence,signal
R16115 T21356 T21358 nsubjpass signal,recorded
R16116 T21357 T21358 auxpass was,recorded
R16117 T21358 T21358 ROOT recorded,recorded
R16118 T21359 T21358 advcl using,recorded
R16119 T21360 T21361 det a,luminometer
R1612 T1990 T1989 prep of,activation
R16120 T21361 T21359 dobj luminometer,using
R16121 T21362 T21358 punct .,recorded
R1613 T1991 T1993 det the,pathway
R1614 T1992 T1993 compound PI3-K/AKT,pathway
R1615 T1837 T1838 nsubj PKCα,induces
R1616 T1993 T1990 pobj pathway,of
R1617 T1994 T1989 appos [,activation
R16178 T21451 T21457 compound Annexin,assay
R16179 T21452 T21457 compound V/PI,assay
R1618 T1838 T1838 ROOT induces,induces
R16180 T21453 T21457 compound Apoptosis,assay
R16181 T21454 T21457 compound Assay,assay
R16182 T21455 T21456 compound Cell,apoptosis
R16183 T21456 T21457 compound apoptosis,assay
R16184 T21457 T21459 nsubjpass assay,performed
R16185 T21458 T21459 auxpass was,performed
R16186 T21459 T21459 ROOT performed,performed
R16187 T21460 T21461 mark as,described
R16188 T21461 T21459 advcl described,performed
R16189 T21462 T21465 advmod previously,]
R1619 T1995 T1996 nummod 3,]
R16190 T21463 T21465 nmod [,]
R16191 T21464 T21465 nummod 51,]
R16192 T21465 T21461 npadvmod ],described
R16193 T21466 T21471 csubj using,apoptosis
R16194 T21467 T21469 det the,V
R16195 T21468 T21469 compound Annexin,V
R16196 T21469 T21466 dobj V,using
R16197 T21470 T21471 nsubj FITC,apoptosis
R16198 T21471 T21459 conj apoptosis,performed
R16199 T21472 T21473 compound detection,kit
R162 T194 T190 pobj survival,to
R1620 T1996 T1994 appos ],[
R16200 T21473 T21471 dobj kit,apoptosis
R16201 T21474 T21473 punct (,kit
R16202 T21475 T21476 compound BD,PharMingen
R16203 T21476 T21473 appos PharMingen,kit
R16204 T21477 T21476 punct ",",PharMingen
R16205 T21478 T21479 compound San,Diego
R16206 T21479 T21476 npadvmod Diego,PharMingen
R16207 T21480 T21479 punct ",",Diego
R16208 T21481 T21479 conj CA,Diego
R16209 T21482 T21476 punct ),PharMingen
R1621 T1997 T1981 punct .,reported
R16210 T21483 T21471 punct .,apoptosis
R16211 T21484 T21494 advmod Briefly,incubated
R16212 T21485 T21494 punct ",",incubated
R16213 T21486 T21487 amod human,monocytes
R16214 T21487 T21494 nsubjpass monocytes,incubated
R16215 T21488 T21487 punct (,monocytes
R16216 T21489 T21491 nummod 5,106
R16217 T21490 T21491 nummod ×,106
R16218 T21491 T21487 appos 106,monocytes
R16219 T21492 T21487 punct ),monocytes
R1622 T1998 T2005 prep In,stimulates
R16220 T21493 T21494 auxpass were,incubated
R16221 T21494 T21494 ROOT incubated,incubated
R16222 T21495 T21494 prep with,incubated
R16223 T21496 T21495 pobj inhibitors,with
R16224 T21497 T21496 prep for,inhibitors
R16225 T21498 T21499 nummod 30,minutes
R16226 T21499 T21497 pobj minutes,for
R16227 T21500 T21499 prep in,minutes
R16228 T21501 T21502 compound X-vivo,medium
R16229 T21502 T21500 pobj medium,in
R1623 T1999 T1998 pobj addition,In
R16230 T21503 T21499 cc and,minutes
R16231 T21504 T21494 conj restimulated,incubated
R16232 T21505 T21504 prep with,restimulated
R16233 T21506 T21508 nummod 100,M-CSF
R16234 T21507 T21508 compound ng/ml,M-CSF
R16235 T21508 T21509 compound M-CSF,overnight
R16236 T21509 T21505 pobj overnight,with
R16237 T21510 T21494 punct .,incubated
R16238 T21511 T21512 det The,cells
R16239 T21512 T21514 nsubjpass cells,removed
R1624 T2000 T1999 prep to,addition
R16240 T21513 T21514 auxpass were,removed
R16241 T21514 T21546 ccomp removed,PharMingen
R16242 T21515 T21514 prep from,removed
R16243 T21516 T21518 det the,dish
R16244 T21517 T21518 compound culture,dish
R16245 T21518 T21515 pobj dish,from
R16246 T21519 T21514 advcl using,removed
R16247 T21520 T21519 dobj Accutase,using
R16248 T21521 T21522 punct (,eBioscience
R16249 T21522 T21520 appos eBioscience,Accutase
R1625 T1839 T1838 dobj IL-1α,induces
R16250 T21523 T21522 punct ",",eBioscience
R16251 T21524 T21525 compound San,Diego
R16252 T21525 T21522 npadvmod Diego,eBioscience
R16253 T21526 T21525 punct ",",Diego
R16254 T21527 T21525 conj CA,Diego
R16255 T21528 T21527 punct ),CA
R16256 T21529 T21514 cc and,removed
R16257 T21530 T21514 conj stained,removed
R16258 T21531 T21530 prep with,stained
R16259 T21532 T21533 compound Annexin,V
R1626 T2001 T2002 compound AKT,activation
R16260 T21533 T21531 pobj V,with
R16261 T21534 T21533 conj FITC,V
R16262 T21535 T21534 cc and,FITC
R16263 T21536 T21534 conj PI,FITC
R16264 T21537 T21530 cc and,stained
R16265 T21538 T21530 conj analyzed,stained
R16266 T21539 T21538 agent by,analyzed
R16267 T21540 T21541 compound flow,cytometry
R16268 T21541 T21539 pobj cytometry,by
R16269 T21542 T21543 punct (,FACSCalibur
R1627 T2002 T2000 pobj activation,to
R16270 T21543 T21541 appos FACSCalibur,cytometry
R16271 T21544 T21546 punct ;,PharMingen
R16272 T21545 T21546 compound BD,PharMingen
R16273 T21546 T21546 ROOT PharMingen,PharMingen
R16274 T21547 T21546 punct ),PharMingen
R16275 T21548 T21546 punct .,PharMingen
R16276 T21549 T21550 compound Annexin,V-FITC
R16277 T21550 T21555 nmod V-FITC,cells
R16278 T21551 T21550 cc and,V-FITC
R16279 T21552 T21550 conj PI,V-FITC
R1628 T2003 T2005 punct ",",stimulates
R16280 T21553 T21555 amod double,cells
R16281 T21554 T21555 amod negative,cells
R16282 T21555 T21557 nsubjpass cells,considered
R16283 T21556 T21557 auxpass were,considered
R16284 T21557 T21557 ROOT considered,considered
R16285 T21558 T21559 amod non-apoptotic,cells
R16286 T21559 T21557 oprd cells,considered
R16287 T21560 T21559 prep for,cells
R16288 T21561 T21562 amod statistical,analysis
R16289 T21562 T21560 pobj analysis,for
R1629 T1840 T1839 punct ",",IL-1α
R16290 T21563 T21557 punct .,considered
R163 T195 T184 punct .,reduced
R1630 T2004 T2005 nsubj M-CSF,stimulates
R1631 T2005 T2005 ROOT stimulates,stimulates
R1632 T2006 T2005 dobj PKC,stimulates
R1633 T2007 T2005 prep in,stimulates
R1634 T1841 T1839 conj iNOS,IL-1α
R1635 T2008 T2009 amod human,monocytes
R1636 T2009 T2007 pobj monocytes,in
R1637 T2010 T2009 cc and,monocytes
R1638 T1842 T1841 cc and,iNOS
R1639 T2011 T2009 conj increases,monocytes
R164 T196 T201 prep In,identify
R1640 T2012 T2013 compound NF-κB,DNA
R16400 T21735 T21738 amod Western,Cells
R16401 T21736 T21738 compound Blot,Cells
R16402 T21737 T21738 compound Analysis,Cells
R16403 T21738 T21740 nsubjpass Cells,washed
R16404 T21739 T21740 auxpass were,washed
R16405 T21740 T21740 ROOT washed,washed
R16406 T21741 T21740 prep with,washed
R16407 T21742 T21741 pobj PBS,with
R16408 T21743 T21740 cc and,washed
R16409 T21744 T21740 conj resuspended,washed
R1641 T2013 T2017 det DNA,]
R16410 T21745 T21744 prep in,resuspended
R16411 T21746 T21747 compound cell,lysis
R16412 T21747 T21748 compound lysis,buffer
R16413 T21748 T21745 pobj buffer,in
R16414 T21749 T21740 punct .,washed
R16415 T21750 T21753 punct (,Technology
R16416 T21751 T21753 compound Cell,Technology
R16417 T21752 T21753 compound Signaling,Technology
R16418 T21753 T21756 nsubj Technology,incubated
R16419 T21754 T21753 punct ),Technology
R1642 T2038 T2043 prep In,plays
R16420 T21755 T21753 cc and,Technology
R16421 T21756 T21756 ROOT incubated,incubated
R16422 T21757 T21756 prep for,incubated
R16423 T21758 T21759 nummod 10,minutes
R16424 T21759 T21757 pobj minutes,for
R16425 T21760 T21759 prep on,minutes
R16426 T21761 T21760 pobj ice,on
R16427 T21762 T21763 advmod then,centrifuged
R16428 T21763 T21756 conj centrifuged,incubated
R16429 T21764 T21765 aux to,remove
R1643 T2014 T2017 amod binding,]
R16430 T21765 T21763 xcomp remove,centrifuged
R16431 T21766 T21768 det the,fraction
R16432 T21767 T21768 amod insoluble,fraction
R16433 T21768 T21765 dobj fraction,remove
R16434 T21769 T21756 punct .,incubated
R16435 T21770 T21772 det The,concentration
R16436 T21771 T21772 compound protein,concentration
R16437 T21772 T21774 nsubjpass concentration,determined
R16438 T21773 T21774 auxpass was,determined
R16439 T21774 T21774 ROOT determined,determined
R1644 T2015 T2017 nmod [,]
R16440 T21775 T21774 agent by,determined
R16441 T21776 T21779 det the,assay
R16442 T21777 T21779 compound BCA,assay
R16443 T21778 T21779 compound protein,assay
R16444 T21779 T21775 pobj assay,by
R16445 T21780 T21781 punct (,Bio-Rad
R16446 T21781 T21779 appos Bio-Rad,assay
R16447 T21782 T21781 punct ",",Bio-Rad
R16448 T21783 T21781 conj Hercules,Bio-Rad
R16449 T21784 T21783 punct ",",Hercules
R1645 T2016 T2017 nummod 34,]
R16450 T21785 T21783 conj CA,Hercules
R16451 T21786 T21781 punct ),Bio-Rad
R16452 T21787 T21774 punct .,determined
R16453 T21788 T21789 compound Cell,lysates
R16454 T21789 T21791 nsubjpass lysates,separated
R16455 T21790 T21791 auxpass were,separated
R16456 T21791 T21791 ROOT separated,separated
R16457 T21792 T21791 agent by,separated
R16458 T21793 T21792 pobj SDS-PAGE,by
R16459 T21794 T21791 prep on,separated
R1646 T2017 T2005 dobj ],stimulates
R16460 T21795 T21796 nummod 10,%
R16461 T21796 T21798 nmod %,gels
R16462 T21797 T21798 compound polyacrylamide,gels
R16463 T21798 T21794 pobj gels,on
R16464 T21799 T21791 cc and,separated
R16465 T21800 T21801 advmod then,transferred
R16466 T21801 T21791 conj transferred,separated
R16467 T21802 T21801 prep onto,transferred
R16468 T21803 T21804 amod nitrocellulose,membranes
R16469 T21804 T21802 pobj membranes,onto
R1647 T2039 T2040 amod other,cells
R16470 T21805 T21801 cc and,transferred
R16471 T21806 T21801 conj subjected,transferred
R16472 T21807 T21806 prep to,subjected
R16473 T21808 T21809 amod Western,blotting
R16474 T21809 T21807 pobj blotting,to
R16475 T21810 T21791 punct .,separated
R16476 T21811 T21813 det The,proteins
R16477 T21812 T21813 amod immunoblotted,proteins
R16478 T21813 T21815 nsubjpass proteins,detected
R16479 T21814 T21815 auxpass were,detected
R1648 T2018 T2005 punct .,stimulates
R16480 T21815 T21815 ROOT detected,detected
R16481 T21816 T21815 agent by,detected
R16482 T21817 T21818 compound ECL,reagent
R16483 T21818 T21816 pobj reagent,by
R16484 T21819 T21823 punct (,Corp.
R16485 T21820 T21823 compound GE,Corp.
R16486 T21821 T21823 compound Healthcare,Corp.
R16487 T21822 T21823 compound Bio-Sciences,Corp.
R16488 T21823 T21818 appos Corp.,reagent
R16489 T21824 T21823 punct ",",Corp.
R1649 T2019 T2035 advmod However,is
R16490 T21825 T21823 npadvmod Piscataway,Corp.
R16491 T21826 T21825 punct ",",Piscataway
R16492 T21827 T21825 conj NJ,Piscataway
R16493 T21828 T21823 punct ),Corp.
R16494 T21829 T21815 punct .,detected
R165 T197 T198 det this,report
R1650 T2020 T2035 punct ",",is
R1651 T2021 T2026 mark whether,is
R1652 T2040 T2038 pobj cells,In
R1653 T2022 T2025 poss PKC,activation
R1654 T2023 T2022 cc and/or,PKC
R1655 T2024 T2022 conj NF-κB,PKC
R1656 T2041 T2043 punct ",",plays
R1657 T2025 T2026 nsubj activation,is
R1658 T2026 T2035 csubj is,is
R16589 T21944 T21952 nsubjpass Analysis,starved
R1659 T2027 T2026 acomp critical,is
R16590 T21945 T21944 prep of,Analysis
R16591 T21946 T21950 compound PKCα,MDMs
R16592 T21947 T21950 compound Kinase,MDMs
R16593 T21948 T21950 compound Activity,MDMs
R16594 T21949 T21950 compound Human,MDMs
R16595 T21950 T21945 pobj MDMs,of
R16596 T21951 T21952 auxpass were,starved
R16597 T21952 T21952 ROOT starved,starved
R16598 T21953 T21952 prep for,starved
R16599 T21954 T21955 nummod 2,hours
R166 T198 T196 pobj report,In
R1660 T2028 T2027 prep in,critical
R16600 T21955 T21953 pobj hours,for
R16601 T21956 T21952 prep before,starved
R16602 T21957 T21956 pobj stimulation,before
R16603 T21958 T21957 prep with,stimulation
R16604 T21959 T21958 pobj M-CSF,with
R16605 T21960 T21959 punct (,M-CSF
R16606 T21961 T21959 nummod 0,M-CSF
R16607 T21962 T21963 nummod 60,minutes
R16608 T21963 T21959 appos minutes,M-CSF
R16609 T21964 T21959 punct ),M-CSF
R1661 T2042 T2043 nsubj PKC,plays
R16610 T21965 T21967 punct ",",washed
R16611 T21966 T21967 advmod then,washed
R16612 T21967 T21952 dep washed,starved
R16613 T21968 T21967 prep with,washed
R16614 T21969 T21970 amod cold,PBS
R16615 T21970 T21968 pobj PBS,with
R16616 T21971 T21967 cc and,washed
R16617 T21972 T21967 conj removed,washed
R16618 T21973 T21972 prep from,removed
R16619 T21974 T21975 det the,plates
R1662 T2029 T2032 amod M-CSF-stimulated,survival
R16620 T21975 T21973 pobj plates,from
R16621 T21976 T21967 punct .,washed
R16622 T21977 T21978 det The,cells
R16623 T21978 T21980 nsubjpass cells,resuspended
R16624 T21979 T21980 auxpass were,resuspended
R16625 T21980 T21980 ROOT resuspended,resuspended
R16626 T21981 T21980 prep in,resuspended
R16627 T21982 T21983 compound lysis,buffer
R16628 T21983 T21981 pobj buffer,in
R16629 T21984 T21987 punct (,Tris
R1663 T2030 T2032 nmod mononuclear,survival
R16630 T21985 T21987 nummod 20,Tris
R16631 T21986 T21987 compound mM,Tris
R16632 T21987 T21987 ROOT Tris,Tris
R16633 T21988 T21987 punct ",",Tris
R16634 T21989 T21991 nummod 5,MgCl2
R16635 T21990 T21991 compound mM,MgCl2
R16636 T21991 T21987 appos MgCl2,Tris
R16637 T21992 T21991 punct ",",MgCl2
R16638 T21993 T21995 nummod 1,EGTA
R16639 T21994 T21995 compound mM,EGTA
R1664 T2031 T2032 compound phagocyte,survival
R16640 T21995 T21991 appos EGTA,MgCl2
R16641 T21996 T21995 punct ",",EGTA
R16642 T21997 T22000 nummod 20,phosphate
R16643 T21998 T22000 nmod mM,phosphate
R16644 T21999 T22000 amod β-glycerol,phosphate
R16645 T22000 T21995 appos phosphate,EGTA
R16646 T22001 T22000 punct ",",phosphate
R16647 T22002 T22021 nsubj 1,sonicated
R16648 T22003 T22009 nmod mM,aprotinin
R16649 T22004 T22009 nmod PMSF,aprotinin
R1665 T2043 T2043 ROOT plays,plays
R16650 T22005 T22006 nummod 2,µg
R16651 T22006 T22009 nmod µg,aprotinin
R16652 T22007 T22009 nmod /,aprotinin
R16653 T22008 T22009 compound ml,aprotinin
R16654 T22009 T22021 nsubj aprotinin,sonicated
R16655 T22010 T22009 cc and,aprotinin
R16656 T22011 T22012 nummod 2,µg
R16657 T22012 T22015 nmod µg,leupeptin
R16658 T22013 T22015 nmod /,leupeptin
R16659 T22014 T22015 compound ml,leupeptin
R1666 T2032 T2028 pobj survival,in
R16660 T22015 T22009 conj leupeptin,aprotinin
R16661 T22016 T22015 punct ",",leupeptin
R16662 T22017 T22015 appos pH,leupeptin
R16663 T22018 T22017 nummod 7.5,pH
R16664 T22019 T22015 punct ),leupeptin
R16665 T22020 T22015 punct ",",leupeptin
R16666 T22021 T22000 conj sonicated,phosphate
R16667 T22022 T22021 cc and,sonicated
R16668 T22023 T22021 conj centrifuged,sonicated
R16669 T22024 T22025 aux to,obtain
R1667 T2033 T2032 cc and/or,survival
R16670 T22025 T22023 advcl obtain,centrifuged
R16671 T22026 T22028 amod whole,lysates
R16672 T22027 T22028 compound cell,lysates
R16673 T22028 T22025 dobj lysates,obtain
R16674 T22029 T22028 punct ",",lysates
R16675 T22030 T22033 nsubjpass which,immunoprecipitated
R16676 T22031 T22033 auxpass were,immunoprecipitated
R16677 T22032 T22033 advmod then,immunoprecipitated
R16678 T22033 T22028 relcl immunoprecipitated,lysates
R16679 T22034 T22033 prep with,immunoprecipitated
R1668 T2034 T2032 conj differentiation,survival
R16680 T22035 T22036 amod anti-PKCα,antibody
R16681 T22036 T22034 pobj antibody,with
R16682 T22037 T22036 cc and,antibody
R16683 T22038 T22039 compound protein,G-agarose
R16684 T22039 T22036 conj G-agarose,antibody
R16685 T22040 T22041 punct (,Invitrogen
R16686 T22041 T22039 appos Invitrogen,G-agarose
R16687 T22042 T22039 punct ),G-agarose
R16688 T22043 T22021 punct .,sonicated
R16689 T22044 T22045 compound Immune,complexes
R1669 T2044 T2046 det an,role
R16690 T22045 T22047 nsubjpass complexes,washed
R16691 T22046 T22047 auxpass were,washed
R16692 T22047 T22047 ROOT washed,washed
R16693 T22048 T22047 advmod twice,washed
R16694 T22049 T22047 punct ",",washed
R16695 T22050 T22047 cc and,washed
R16696 T22051 T22052 compound PKCα,activity
R16697 T22052 T22054 nsubjpass activity,analyzed
R16698 T22053 T22054 auxpass was,analyzed
R16699 T22054 T22047 conj analyzed,washed
R167 T199 T201 punct ",",identify
R1670 T2035 T2035 ROOT is,is
R16700 T22055 T22054 agent by,analyzed
R16701 T22056 T22059 amod non-radioactive,kit
R16702 T22057 T22059 compound Peptag,kit
R16703 T22058 T22059 compound assay,kit
R16704 T22059 T22055 pobj kit,by
R16705 T22060 T22059 punct (,kit
R16706 T22061 T22059 appos Promega,kit
R16707 T22062 T22059 punct ),kit
R16708 T22063 T22054 punct .,analyzed
R16709 T22064 T22078 advmod Briefly,incubated
R1671 T2036 T2035 acomp unclear,is
R16710 T22065 T22078 punct ",",incubated
R16711 T22066 T22067 compound kinase,buffer
R16712 T22067 T22078 nsubjpass buffer,incubated
R16713 T22068 T22067 punct ",",buffer
R16714 T22069 T22067 conj activator,buffer
R16715 T22070 T22069 punct ",",activator
R16716 T22071 T22072 compound peptide,protection
R16717 T22072 T22073 compound protection,solution
R16718 T22073 T22069 conj solution,activator
R16719 T22074 T22073 cc and,solution
R1672 T2037 T2035 punct .,is
R16720 T22075 T22076 compound Peptag,peptide
R16721 T22076 T22073 conj peptide,solution
R16722 T22077 T22078 auxpass were,incubated
R16723 T22078 T22078 ROOT incubated,incubated
R16724 T22079 T22078 prep with,incubated
R16725 T22080 T22082 det the,complexes
R16726 T22081 T22082 amod immune,complexes
R16727 T22082 T22079 pobj complexes,with
R16728 T22083 T22082 prep at,complexes
R16729 T22084 T22085 compound 30,°
R1673 T2045 T2046 amod important,role
R16730 T22085 T22086 nummod °,C
R16731 T22086 T22083 pobj C,at
R16732 T22087 T22078 prep for,incubated
R16733 T22088 T22089 nummod 30,minutes
R16734 T22089 T22087 pobj minutes,for
R16735 T22090 T22078 punct .,incubated
R16736 T22091 T22093 nsubjpass Reactions,stopped
R16737 T22092 T22093 auxpass were,stopped
R16738 T22093 T22093 ROOT stopped,stopped
R16739 T22094 T22093 prep by,stopped
R1674 T2046 T2043 dobj role,plays
R16740 T22095 T22094 pcomp boiling,by
R16741 T22096 T22095 prep for,boiling
R16742 T22097 T22098 nummod 10,minutes
R16743 T22098 T22096 pobj minutes,for
R16744 T22099 T22098 cc and,minutes
R16745 T22100 T22098 conj samples,minutes
R16746 T22101 T22102 auxpass were,separated
R16747 T22102 T22093 advcl separated,stopped
R16748 T22103 T22102 prep on,separated
R16749 T22104 T22105 nummod 0.8,%
R1675 T2047 T2046 prep in,role
R16750 T22105 T22107 nmod %,gel
R16751 T22106 T22107 amod agarose,gel
R16752 T22107 T22103 pobj gel,on
R16753 T22108 T22093 punct .,stopped
R16754 T22109 T22110 compound Phosphorylated,peptide
R16755 T22110 T22111 nsubj peptide,migrated
R16756 T22111 T22111 ROOT migrated,migrated
R16757 T22112 T22111 prep toward,migrated
R16758 T22113 T22114 det the,anode
R16759 T22114 T22112 pobj anode,toward
R1676 T2135 T2132 conj siRNA,constructs
R16760 T22115 T22114 punct (,anode
R16761 T22116 T22114 parataxis +,anode
R16762 T22117 T22114 punct ),anode
R16763 T22118 T22111 punct ",",migrated
R16764 T22119 T22122 mark while,migrated
R16765 T22120 T22121 compound non-phosphorylated,peptide
R16766 T22121 T22122 nsubj peptide,migrated
R16767 T22122 T22111 advcl migrated,migrated
R16768 T22123 T22122 prep toward,migrated
R16769 T22124 T22125 det the,cathode
R1677 T2048 T2049 amod NF-κB,activation
R16770 T22125 T22123 pobj cathode,toward
R16771 T22126 T22125 punct (,cathode
R16772 T22127 T22125 appos −,cathode
R16773 T22128 T22125 punct ),cathode
R16774 T22129 T22111 punct .,migrated
R16775 T22130 T22131 amod Fluorescein-tagged,peptides
R16776 T22131 T22133 nsubjpass peptides,visualized
R16777 T22132 T22133 auxpass were,visualized
R16778 T22133 T22133 ROOT visualized,visualized
R16779 T22134 T22133 agent by,visualized
R1678 T2136 T2117 punct .,reduced
R16780 T22135 T22136 compound UV,light
R16781 T22136 T22134 pobj light,by
R16782 T22137 T22133 punct .,visualized
R1679 T2137 T2137 ROOT Conventional,Conventional
R168 T200 T201 nsubj we,identify
R1680 T2138 T2139 nsubj PKC,regulated
R1681 T2139 T2139 ROOT regulated,regulated
R1682 T2049 T2047 pobj activation,in
R1683 T2140 T2142 amod NF-κB-regulated,expression
R1684 T2141 T2142 compound gene,expression
R1685 T2050 T2049 cc and,activation
R1686 T2142 T2139 dobj expression,regulated
R1687 T2143 T2142 cc and,expression
R1688 T2051 T2052 compound cell,survival
R1689 T2052 T2049 conj survival,activation
R169 T201 T201 ROOT identify,identify
R1690 T2144 T2142 conj phosphorylation,expression
R1691 T2145 T2142 prep of,expression
R1692 T2053 T2055 nmod [,]
R1693 T2146 T2145 pobj Ser276,of
R1694 T2147 T2146 prep of,Ser276
R1695 T2148 T2147 pobj NF-κB,of
R1696 T2054 T2055 nummod 35,]
R16968 T22378 T22379 compound RNA,Isolation
R16969 T22379 T22379 ROOT Isolation,Isolation
R1697 T2149 T2148 nummod p65,NF-κB
R16970 T22380 T22379 cc and,Isolation
R16971 T22381 T22384 nmod Quantitative,MDMs
R16972 T22382 T22384 amod Real-time,MDMs
R16973 T22383 T22384 compound PCR,MDMs
R16974 T22384 T22379 conj MDMs,Isolation
R16975 T22385 T22384 cc or,MDMs
R16976 T22386 T22384 conj RAW,MDMs
R16977 T22387 T22388 nummod 264.7,cells
R16978 T22388 T22389 nsubj cells,were
R16979 T22389 T22389 ROOT were,were
R1698 T2150 T2139 conj occurred,regulated
R16980 T22390 T22391 amod serum-starved,overnight
R16981 T22391 T22389 acomp overnight,were
R16982 T22392 T22389 cc or,were
R16983 T22393 T22399 prep for,prior
R16984 T22394 T22395 nummod 2,hours
R16985 T22395 T22393 pobj hours,for
R16986 T22396 T22399 punct ",",prior
R16987 T22397 T22399 advmod respectively,prior
R16988 T22398 T22399 punct ",",prior
R16989 T22399 T22389 conj prior,were
R1699 T2055 T2073 nsubjpass ],known
R16990 T22400 T22399 prep to,prior
R16991 T22401 T22400 pobj incubating,to
R16992 T22402 T22401 prep with,incubating
R16993 T22403 T22402 pobj inhibitors,with
R16994 T22404 T22403 prep for,inhibitors
R16995 T22405 T22406 nummod 30,minutes
R16996 T22406 T22404 pobj minutes,for
R16997 T22407 T22399 punct .,prior
R16998 T22408 T22409 det The,cells
R16999 T22409 T22411 nsubjpass cells,restimulated
R170 T202 T203 amod conventional,PKCs
R1700 T2151 T2150 prep in,occurred
R17000 T22410 T22411 auxpass were,restimulated
R17001 T22411 T22411 ROOT restimulated,restimulated
R17002 T22412 T22411 prep with,restimulated
R17003 T22413 T22415 nummod 100,M-CSF
R17004 T22414 T22415 compound ng/ml,M-CSF
R17005 T22415 T22412 pobj M-CSF,with
R17006 T22416 T22415 cc and,M-CSF
R17007 T22417 T22418 amod total,mRNA
R17008 T22418 T22415 conj mRNA,M-CSF
R17009 T22419 T22420 auxpass was,extracted
R1701 T2152 T2154 det an,fashion
R17010 T22420 T22411 conj extracted,restimulated
R17011 T22421 T22420 prep from,extracted
R17012 T22422 T22421 pobj cells,from
R17013 T22423 T22422 prep with,cells
R17014 T22424 T22423 pobj Trizol,with
R17015 T22425 T22424 punct (,Trizol
R17016 T22426 T22424 appos Invitrogen,Trizol
R17017 T22427 T22424 punct ),Trizol
R17018 T22428 T22422 cc and,cells
R17019 T22429 T22422 conj 1,cells
R1702 T2153 T2154 amod M-CSF-dependent,fashion
R17020 T22430 T22431 nummod 2,µg
R17021 T22431 T22436 nsubjpass µg,used
R17022 T22432 T22431 prep of,µg
R17023 T22433 T22434 amod total,RNA
R17024 T22434 T22432 pobj RNA,of
R17025 T22435 T22436 auxpass was,used
R17026 T22436 T22436 ROOT used,used
R17027 T22437 T22436 prep to,used
R17028 T22438 T22439 amod synthesized,cDNA
R17029 T22439 T22437 pobj cDNA,to
R1703 T2056 T2060 punct ",",mechanisms
R17030 T22440 T22436 advcl using,used
R17031 T22441 T22442 compound SuperScript,III
R17032 T22442 T22440 dobj III,using
R17033 T22443 T22442 punct (,III
R17034 T22444 T22442 appos Invitrogen,III
R17035 T22445 T22442 punct ),III
R17036 T22446 T22436 punct .,used
R17037 T22447 T22455 amod Quantitative,performed
R17038 T22448 T22455 npadvmod real-time,performed
R17039 T22449 T22450 punct (,qRT
R1704 T2154 T2151 pobj fashion,in
R17040 T22450 T22448 appos qRT,real-time
R17041 T22451 T22450 punct ),qRT
R17042 T22452 T22453 punct -,PCR
R17043 T22453 T22455 nsubjpass PCR,performed
R17044 T22454 T22455 auxpass was,performed
R17045 T22455 T22455 ROOT performed,performed
R17046 T22456 T22455 advcl using,performed
R17047 T22457 T22460 compound SYBR,Mix
R17048 T22458 T22460 compound Green,Mix
R17049 T22459 T22460 compound Master,Mix
R1705 T2155 T2154 acl correlating,fashion
R17050 T22460 T22456 dobj Mix,using
R17051 T22461 T22460 punct (,Mix
R17052 T22462 T22463 compound Applied,BioSystems
R17053 T22463 T22460 appos BioSystems,Mix
R17054 T22464 T22463 punct ",",BioSystems
R17055 T22465 T22463 conj Carlsbad,BioSystems
R17056 T22466 T22465 punct ",",Carlsbad
R17057 T22467 T22465 conj CA,Carlsbad
R17058 T22468 T22460 punct ),Mix
R17059 T22469 T22455 punct .,performed
R1706 T2156 T2155 prep with,correlating
R17060 T22470 T22471 det The,reactions
R17061 T22471 T22473 nsubjpass reactions,performed
R17062 T22472 T22473 auxpass were,performed
R17063 T22473 T22473 ROOT performed,performed
R17064 T22474 T22473 advcl using,performed
R17065 T22475 T22479 det an,machine
R17066 T22476 T22477 compound ABI,PRIZM
R17067 T22477 T22479 nmod PRIZM,machine
R17068 T22478 T22479 nummod 7700,machine
R17069 T22479 T22474 dobj machine,using
R1707 T2057 T2060 advmod however,mechanisms
R17070 T22480 T22474 prep with,using
R17071 T22481 T22483 compound software,Detector
R17072 T22482 T22483 compound Sequence,Detector
R17073 T22483 T22484 compound Detector,version
R17074 T22484 T22480 pobj version,with
R17075 T22485 T22484 nummod 1.7,version
R17076 T22486 T22488 punct (,Biosystems
R17077 T22487 T22488 compound Applied,Biosystems
R17078 T22488 T22484 appos Biosystems,version
R17079 T22489 T22473 punct ),performed
R1708 T2157 T2160 poss its,activity
R17080 T22490 T22473 punct .,performed
R17081 T22491 T22494 det The,values
R17082 T22492 T22493 compound target,gene
R17083 T22493 T22494 compound gene,values
R17084 T22494 T22495 nsubj values,were
R17085 T22495 T22495 ROOT were,were
R17086 T22496 T22495 acomp normalized,were
R17087 T22497 T22496 prep to,normalized
R17088 T22498 T22499 det the,values
R17089 T22499 T22497 pobj values,to
R1709 T2158 T2160 amod maximal,activity
R17090 T22500 T22499 prep of,values
R17091 T22501 T22500 pobj GAPDH,of
R17092 T22502 T22496 prep as,normalized
R17093 T22503 T22505 det a,gene
R17094 T22504 T22505 compound housekeeping,gene
R17095 T22505 T22502 pobj gene,as
R17096 T22506 T22496 cc and,normalized
R17097 T22507 T22496 conj expressed,normalized
R17098 T22508 T22507 prep as,expressed
R17099 T22509 T22511 amod relative,increase
R171 T203 T201 dobj PKCs,identify
R1710 T2058 T2060 det the,mechanisms
R17100 T22510 T22511 compound fold,increase
R17101 T22511 T22508 pobj increase,as
R17102 T22512 T22511 nummod 2,increase
R17103 T22513 T22515 punct (,ΔΔCt
R17104 T22514 T22515 compound −,ΔΔCt
R17105 T22515 T22511 appos ΔΔCt,increase
R17106 T22516 T22515 punct ),ΔΔCt
R17107 T22517 T22511 prep over,increase
R17108 T22518 T22520 det the,samples
R17109 T22519 T22520 amod non-stimulated,samples
R1711 T2159 T2160 amod transcriptional,activity
R17110 T22520 T22517 pobj samples,over
R17111 T22521 T22522 punct (,NS
R17112 T22522 T22520 appos NS,samples
R17113 T22523 T22495 punct ),were
R17114 T22524 T22495 punct .,were
R1712 T2160 T2156 pobj activity,with
R1713 T2161 T2139 punct .,regulated
R1714 T2059 T2060 amod specific,mechanisms
R1715 T2162 T2171 advmod Furthermore,plays
R1716 T2163 T2171 punct ",",plays
R1717 T2164 T2165 amod PKCα-regulated,phosphorylation
R1718 T2060 T2073 nsubjpass mechanisms,known
R1719 T2165 T2171 nsubj phosphorylation,plays
R172 T204 T203 punct ",",PKCs
R1720 T2166 T2165 prep of,phosphorylation
R1721 T2167 T2166 pobj Ser276,of
R1722 T2168 T2167 prep of,Ser276
R1723 T2169 T2168 pobj NF-κB,of
R1724 T2170 T2169 nummod p65,NF-κB
R1725 T2171 T2171 ROOT plays,plays
R1726 T2061 T2060 prep of,mechanisms
R1727 T2172 T2174 det an,role
R1728 T2173 T2174 amod important,role
R17289 T22732 T22733 compound Ethics,Statement
R1729 T2174 T2171 dobj role,plays
R17290 T22733 T22735 compound Statement,research
R17291 T22734 T22735 compound All,research
R17292 T22735 T22744 nsubjpass research,ordered
R17293 T22736 T22735 acl involving,research
R17294 T22737 T22738 amod human,blood
R17295 T22738 T22736 dobj blood,involving
R17296 T22739 T22741 punct (,coat
R17297 T22740 T22741 compound Buffy,coat
R17298 T22741 T22738 appos coat,blood
R17299 T22742 T22735 punct ),research
R173 T205 T203 prep including,PKCs
R1730 T2175 T2174 prep in,role
R17300 T22743 T22744 auxpass were,ordered
R17301 T22744 T22744 ROOT ordered,ordered
R17302 T22745 T22744 prep from,ordered
R17303 T22746 T22748 amod American,cross
R17304 T22747 T22748 compound Red,cross
R17305 T22748 T22745 pobj cross,from
R17306 T22749 T22744 cc and,ordered
R17307 T22750 T22752 aux have,approved
R17308 T22751 T22752 auxpass been,approved
R17309 T22752 T22744 conj approved,ordered
R1731 T2062 T2063 det this,activation
R17310 T22753 T22752 agent by,approved
R17311 T22754 T22759 det the,board
R17312 T22755 T22757 compound Ohio,University
R17313 T22756 T22757 compound State,University
R17314 T22757 T22759 compound University,board
R17315 T22758 T22759 compound review,board
R17316 T22759 T22753 pobj board,by
R17317 T22760 T22763 punct (,Protocol
R17318 T22761 T22763 compound ARC,Protocol
R17319 T22762 T22763 compound IRB,Protocol
R1732 T2176 T2175 pcomp regulating,in
R17320 T22763 T22759 appos Protocol,board
R17321 T22764 T22763 nummod #,Protocol
R17322 T22765 T22764 npadvmod 2001-26,#
R17323 T22766 T22763 punct ),Protocol
R17324 T22767 T22744 punct .,ordered
R1733 T2177 T2178 poss its,activity
R1734 T2178 T2176 dobj activity,regulating
R1735 T2063 T2061 pobj activation,of
R1736 T2179 T2176 prep in,regulating
R17362 T22818 T22819 nsubj M-CSF,induces
R17363 T22819 T22819 ROOT induces,induces
R17364 T22820 T22822 amod NF-κB,activity
R17365 T22821 T22822 amod transcriptional,activity
R17366 T22822 T22819 dobj activity,induces
R17367 T22823 T22822 prep in,activity
R17368 T22824 T22823 pobj macrophages,in
R17369 T22825 T22819 punct .,induces
R1737 T2180 T2181 compound mononuclear,phagocytes
R17370 T22826 T22831 punct (,extracted
R17371 T22827 T22829 nmod A,Nuclei
R17372 T22828 T22827 punct ),A
R17373 T22829 T22831 nsubjpass Nuclei,extracted
R17374 T22830 T22831 auxpass were,extracted
R17375 T22831 T22831 ROOT extracted,extracted
R17376 T22832 T22831 prep from,extracted
R17377 T22833 T22834 amod human,MDMs
R17378 T22834 T22832 pobj MDMs,from
R17379 T22835 T22831 conj treated,extracted
R1738 T2181 T2179 pobj phagocytes,in
R17380 T22836 T22835 prep without,treated
R17381 T22837 T22836 cc or,without
R17382 T22838 T22836 conj with,without
R17383 T22839 T22838 pobj M-CSF,with
R17384 T22840 T22835 prep for,treated
R17385 T22841 T22844 nummod 15,minutes
R17386 T22842 T22841 cc or,15
R17387 T22843 T22841 conj 30,15
R17388 T22844 T22840 pobj minutes,for
R17389 T22845 T22831 punct .,extracted
R1739 T2064 T2063 cc and,activation
R17390 T22846 T22847 compound NF-κB,DNA
R17391 T22847 T22849 compound DNA,activity
R17392 T22848 T22849 amod binding,activity
R17393 T22849 T22851 nsubjpass activity,analyzed
R17394 T22850 T22851 auxpass was,analyzed
R17395 T22851 T22851 ROOT analyzed,analyzed
R17396 T22852 T22851 agent by,analyzed
R17397 T22853 T22854 compound EMSA,Shown
R17398 T22854 T22852 pobj Shown,by
R17399 T22855 T22851 conj is,analyzed
R174 T206 T205 pobj PKCα,including
R1740 T2182 T2181 cc and,phagocytes
R17400 T22856 T22858 det a,blot
R17401 T22857 T22858 amod representative,blot
R17402 T22858 T22855 attr blot,is
R17403 T22859 T22858 prep from,blot
R17404 T22860 T22862 nummod three,experiments
R17405 T22861 T22862 amod independent,experiments
R17406 T22862 T22859 pobj experiments,from
R17407 T22863 T22851 punct .,analyzed
R17408 T22864 T22864 ROOT NS,NS
R17409 T22865 T22864 punct :,NS
R1741 T2183 T2185 amod murine,fibroblasts
R17410 T22866 T22864 acl non-stimulated,NS
R17411 T22867 T22864 punct .,NS
R17412 T22868 T22874 punct (,transfected
R17413 T22869 T22874 dep B,transfected
R17414 T22870 T22869 punct ),B
R17415 T22871 T22872 compound Human,MDMs
R17416 T22872 T22874 nsubj MDMs,transfected
R17417 T22873 T22874 advmod transiently,transfected
R17418 T22874 T22874 ROOT transfected,transfected
R17419 T22875 T22874 prep with,transfected
R1742 T2184 T2185 amod embryonic,fibroblasts
R17420 T22876 T22879 amod pTAL-SEAP,constructs
R17421 T22877 T22876 cc or,pTAL-SEAP
R17422 T22878 T22876 conj pNF-κB-SEAP,pTAL-SEAP
R17423 T22879 T22875 pobj constructs,with
R17424 T22880 T22881 auxpass were,treated
R17425 T22881 T22874 dep treated,transfected
R17426 T22882 T22881 prep with,treated
R17427 T22883 T22882 pobj M-CSF,with
R17428 T22884 T22883 prep in,M-CSF
R17429 T22885 T22886 compound X-vivo,medium
R1743 T2065 T2067 det the,effects
R17430 T22886 T22884 pobj medium,in
R17431 T22887 T22881 cc and,treated
R17432 T22888 T22881 conj incubated,treated
R17433 T22889 T22888 prep for,incubated
R17434 T22890 T22891 nummod 6,hours
R17435 T22891 T22889 pobj hours,for
R17436 T22892 T22888 advmod before,incubated
R17437 T22893 T22894 det the,collection
R17438 T22894 T22892 pobj collection,before
R17439 T22895 T22894 prep of,collection
R1744 T2185 T2181 conj fibroblasts,phagocytes
R17440 T22896 T22895 pobj medium,of
R17441 T22897 T22874 punct .,transfected
R17442 T22898 T22899 amod NF-κB,activity
R17443 T22899 T22901 nsubjpass activity,analyzed
R17444 T22900 T22901 auxpass was,analyzed
R17445 T22901 T22901 ROOT analyzed,analyzed
R17446 T22902 T22901 prep by,analyzed
R17447 T22903 T22902 pcomp measuring,by
R17448 T22904 T22905 det the,amount
R17449 T22905 T22903 dobj amount,measuring
R1745 T2186 T2171 punct .,plays
R17450 T22906 T22905 prep of,amount
R17451 T22907 T22906 pobj SEAP,of
R17452 T22908 T22901 conj secreted,analyzed
R17453 T22909 T22908 prep into,secreted
R17454 T22910 T22911 det the,medium
R17455 T22911 T22909 pobj medium,into
R17456 T22912 T22911 cc and,medium
R17457 T22913 T22911 conj data,medium
R17458 T22914 T22915 auxpass are,expressed
R17459 T22915 T22915 ROOT expressed,expressed
R1746 T2066 T2067 amod biological,effects
R17460 T22916 T22915 prep as,expressed
R17461 T22917 T22918 compound fold,increase
R17462 T22918 T22916 pobj increase,as
R17463 T22919 T22918 prep of,increase
R17464 T22920 T22921 compound SEAP,activity
R17465 T22921 T22919 pobj activity,of
R17466 T22922 T22921 prep over,activity
R17467 T22923 T22922 pobj that,over
R17468 T22924 T22921 prep in,activity
R17469 T22925 T22928 amod pTAL-SEAP,cells
R1747 T2067 T2063 conj effects,activation
R17470 T22926 T22928 amod transfected,cells
R17471 T22927 T22928 amod resting,cells
R17472 T22928 T22924 pobj cells,in
R17473 T22929 T22901 punct .,analyzed
R17474 T22930 T22933 punct (,RAW
R17475 T22931 T22933 nmod C,RAW
R17476 T22932 T22933 punct ),RAW
R17477 T22933 T22938 nsubjpass RAW,transfected
R17478 T22934 T22935 nummod 264.7,cells
R17479 T22935 T22938 nsubjpass cells,transfected
R1748 T2068 T2067 prep on,effects
R17480 T22936 T22938 auxpass were,transfected
R17481 T22937 T22938 advmod transiently,transfected
R17482 T22938 T22938 ROOT transfected,transfected
R17483 T22939 T22938 prep with,transfected
R17484 T22940 T22939 pobj pTAL-SEAP,with
R17485 T22941 T22940 cc or,pTAL-SEAP
R17486 T22942 T22940 conj pNF-κB-SEAP,pTAL-SEAP
R17487 T22943 T22938 conj construct,transfected
R17488 T22944 T22938 punct .,transfected
R17489 T22945 T22948 nsubjpass Cells,starved
R1749 T2069 T2070 amod cellular,phenotype
R17490 T22946 T22948 auxpass were,starved
R17491 T22947 T22948 advmod serum,starved
R17492 T22948 T22948 ROOT starved,starved
R17493 T22949 T22948 prep for,starved
R17494 T22950 T22951 nummod 4,hours
R17495 T22951 T22949 pobj hours,for
R17496 T22952 T22948 advmod prior,starved
R17497 T22953 T22952 prep to,prior
R17498 T22954 T22955 nummod 2,hours
R17499 T22955 T22953 pobj hours,to
R175 T207 T201 prep as,identify
R1750 T2070 T2068 pobj phenotype,on
R17500 T22956 T22948 advcl stimulation,starved
R17501 T22957 T22956 prep with,stimulation
R17502 T22958 T22960 amod mouse,M-CSF
R17503 T22959 T22960 amod recombinant,M-CSF
R17504 T22960 T22957 pobj M-CSF,with
R17505 T22961 T22963 punct (,ng/ml
R17506 T22962 T22963 nummod 100,ng/ml
R17507 T22963 T22960 appos ng/ml,M-CSF
R17508 T22964 T22963 punct ),ng/ml
R17509 T22965 T22948 punct .,starved
R1751 T2071 T2073 auxpass are,known
R17510 T22966 T22967 compound Culture,media
R17511 T22967 T22969 nsubjpass media,collected
R17512 T22968 T22969 auxpass was,collected
R17513 T22969 T22969 ROOT collected,collected
R17514 T22970 T22971 aux to,measure
R17515 T22971 T22969 xcomp measure,collected
R17516 T22972 T22973 compound SEAP,production
R17517 T22973 T22971 dobj production,measure
R17518 T22974 T22969 punct .,collected
R17519 T22975 T22977 nsubjpass Data,expressed
R1752 T2072 T2073 neg not,known
R17520 T22976 T22977 auxpass are,expressed
R17521 T22977 T22977 ROOT expressed,expressed
R17522 T22978 T22980 amod mean,S.E.M
R17523 T22979 T22980 compound ±,S.E.M
R17524 T22980 T22977 dobj S.E.M,expressed
R17525 T22981 T22977 punct ",",expressed
R17526 T22982 T22977 prep for,expressed
R17527 T22983 T22985 nummod three,experiments
R17528 T22984 T22985 amod independent,experiments
R17529 T22985 T22982 pobj experiments,for
R1753 T2073 T2073 ROOT known,known
R17530 T22986 T22977 punct .,expressed
R176 T208 T210 amod important,kinases
R1760 T2074 T2073 punct .,known
R1761 T2075 T2078 advmod Therefore,focused
R1767 T2076 T2078 punct ",",focused
R177 T209 T210 compound upstream,kinases
R1770 T2077 T2078 nsubj we,focused
R17701 T23222 T23225 nsubj Inhibition,reduces
R17702 T23223 T23222 prep of,Inhibition
R17703 T23224 T23223 pobj PKC,of
R17704 T23225 T23225 ROOT reduces,reduces
R17705 T23226 T23227 amod NF-κB,activity
R17706 T23227 T23225 dobj activity,reduces
R17707 T23228 T23225 prep in,reduces
R17708 T23229 T23228 pobj M-CSF,in
R17709 T23230 T23225 advcl stimulated,reduces
R17710 T23231 T23230 dobj macrophages,stimulated
R17711 T23232 T23225 punct .,reduces
R17712 T23233 T23233 ROOT (,(
R17713 T23234 T23233 dep A,(
R17714 T23235 T23234 punct ),A
R17715 T23236 T23237 compound Human,MDMs
R17716 T23237 T23238 nsubj MDMs,transfected
R17717 T23238 T23238 ROOT transfected,transfected
R17718 T23239 T23238 prep with,transfected
R17719 T23240 T23239 pobj pNF-κB-SEAP,with
R17720 T23241 T23242 auxpass were,pre-incubated
R17721 T23242 T23238 conj pre-incubated,transfected
R17722 T23243 T23242 prep with,pre-incubated
R17723 T23244 T23243 pobj inhibitors,with
R17724 T23245 T23255 punct ;,prior
R17725 T23246 T23255 nsubj Ro-31-8220,prior
R17726 T23247 T23246 punct ",",Ro-31-8220
R17727 T23248 T23246 conj Gö-6976,Ro-31-8220
R17728 T23249 T23248 punct ",",Gö-6976
R17729 T23250 T23248 cc or,Gö-6976
R17730 T23251 T23248 conj BAPTA/AM,Gö-6976
R17731 T23252 T23246 prep for,Ro-31-8220
R17732 T23253 T23254 nummod 30,minutes
R17733 T23254 T23252 pobj minutes,for
R17734 T23255 T23260 amod prior,stimulation
R17735 T23256 T23258 quantmod to,hours
R17736 T23257 T23258 nummod 6,hours
R17737 T23258 T23259 npadvmod hours,M-CSF
R17738 T23259 T23260 compound M-CSF,stimulation
R17739 T23260 T23242 npadvmod stimulation,pre-incubated
R17740 T23261 T23238 punct .,transfected
R17741 T23262 T23265 punct (,RAW
R17742 T23263 T23265 nmod B,RAW
R17743 T23264 T23265 punct ),RAW
R17744 T23265 T23272 npadvmod RAW,construct
R17745 T23266 T23265 nummod 264.7,RAW
R17746 T23267 T23272 nsubj cells,construct
R17747 T23268 T23267 acl transfected,cells
R17748 T23269 T23268 prep with,transfected
R17749 T23270 T23271 det the,pNF-κB-SEAP
R17750 T23271 T23269 pobj pNF-κB-SEAP,with
R17751 T23272 T23272 ROOT construct,construct
R17752 T23273 T23274 auxpass were,pre-incubated
R17753 T23274 T23272 ccomp pre-incubated,construct
R17754 T23275 T23274 prep with,pre-incubated
R17755 T23276 T23275 pobj inhibitors,with
R17756 T23277 T23276 prep for,inhibitors
R17757 T23278 T23279 nummod 30,minutes
R17758 T23279 T23277 pobj minutes,for
R17759 T23280 T23274 advmod prior,pre-incubated
R1776 T2078 T2078 ROOT focused,focused
R17760 T23281 T23280 prep to,prior
R17761 T23282 T23283 nummod 2,hours
R17762 T23283 T23281 pobj hours,to
R17763 T23284 T23283 prep of,hours
R17764 T23285 T23284 pobj stimulation,of
R17765 T23286 T23274 prep with,pre-incubated
R17766 T23287 T23289 compound mouse,M-CSF
R17767 T23288 T23289 amod recombinant,M-CSF
R17768 T23289 T23286 pobj M-CSF,with
R17769 T23290 T23272 punct .,construct
R17770 T23291 T23293 det The,activity
R17771 T23292 T23293 amod NF-κB,activity
R17772 T23293 T23295 nsubjpass activity,analyzed
R17773 T23294 T23295 auxpass was,analyzed
R17774 T23295 T23295 ROOT analyzed,analyzed
R17775 T23296 T23295 agent by,analyzed
R17776 T23297 T23296 pcomp measuring,by
R17777 T23298 T23299 compound SEAP,production
R17778 T23299 T23297 dobj production,measuring
R17779 T23300 T23299 prep in,production
R17780 T23301 T23302 det the,medium
R17781 T23302 T23300 pobj medium,in
R17782 T23303 T23302 cc and,medium
R17783 T23304 T23302 conj data,medium
R17784 T23305 T23306 auxpass are,expressed
R17785 T23306 T23295 advcl expressed,analyzed
R17786 T23307 T23306 prep as,expressed
R17787 T23308 T23309 compound fold,increase
R17788 T23309 T23307 pobj increase,as
R17789 T23310 T23309 prep of,increase
R17790 T23311 T23312 compound SEAP,activity
R17791 T23312 T23310 pobj activity,of
R17792 T23313 T23312 prep over,activity
R17793 T23314 T23313 pobj that,over
R17794 T23315 T23312 prep in,activity
R17795 T23316 T23319 amod pTAL-SEAP,cells
R17796 T23317 T23319 amod transfected,cells
R17797 T23318 T23319 amod resting,cells
R17798 T23319 T23315 pobj cells,in
R17799 T23320 T23295 punct .,analyzed
R178 T210 T207 pobj kinases,as
R1780 T2079 T2078 prep at,focused
R17800 T23321 T23322 det The,graph
R17801 T23322 T23323 nsubj graph,represents
R17802 T23323 T23323 ROOT represents,represents
R17803 T23324 T23326 amod mean,S.E.M
R17804 T23325 T23326 compound ±,S.E.M
R17805 T23326 T23323 dobj S.E.M,represents
R17806 T23327 T23323 prep for,represents
R17807 T23328 T23330 nummod three,experiments
R17808 T23329 T23330 amod independent,experiments
R17809 T23330 T23327 pobj experiments,for
R17810 T23331 T23323 punct .,represents
R17811 T23332 T23332 ROOT *,*
R17812 T23333 T23334 det The,p-values
R17813 T23334 T23340 nsubj p-values,compared
R17814 T23335 T23334 prep of,p-values
R17815 T23336 T23335 pobj cells,of
R17816 T23337 T23336 acl treated,cells
R17817 T23338 T23337 prep with,treated
R17818 T23339 T23340 amod inhibitors/M-CSF,compared
R17819 T23340 T23340 ROOT compared,compared
R1782 T2080 T2079 pcomp understanding,at
R17820 T23341 T23340 prep to,compared
R17821 T23342 T23341 pobj vehicle/M-CSF,to
R17822 T23343 T23340 auxpass were,compared
R17823 T23344 T23345 compound ≤,0.05
R17824 T23345 T23343 attr 0.05,were
R17825 T23346 T23340 punct .,compared
R179 T211 T210 prep for,kinases
R1791 T2081 T2082 det the,role
R1794 T2082 T2080 dobj role,understanding
R1795 T2083 T2082 prep of,role
R17951 T23560 T23565 nsubj Inhibition,induces
R17952 T23561 T23560 prep of,Inhibition
R17953 T23562 T23561 pobj PKC,of
R17954 T23563 T23562 cc or,PKC
R17955 T23564 T23562 conj NF-κB,PKC
R17956 T23565 T23565 ROOT induces,induces
R17957 T23566 T23565 dobj apoptosis,induces
R17958 T23567 T23565 prep in,induces
R17959 T23568 T23567 pobj MDMs,in
R17960 T23569 T23565 punct .,induces
R17961 T23570 T23575 punct (,pre-incubated
R17962 T23571 T23573 nmod A,MDMs
R17963 T23572 T23573 punct ),MDMs
R17964 T23573 T23575 nsubjpass MDMs,pre-incubated
R17965 T23574 T23575 auxpass were,pre-incubated
R17966 T23575 T23575 ROOT pre-incubated,pre-incubated
R17967 T23576 T23575 prep in,pre-incubated
R17968 T23577 T23578 compound RPMI,medium
R17969 T23578 T23576 pobj medium,in
R17970 T23579 T23578 acl containing,medium
R17971 T23580 T23579 dobj inhibitors,containing
R17972 T23581 T23580 punct (,inhibitors
R17973 T23582 T23580 appos Ro-31-8220,inhibitors
R17974 T23583 T23582 punct :,Ro-31-8220
R17975 T23584 T23585 nummod 5,µM
R17976 T23585 T23582 appos µM,Ro-31-8220
R17977 T23586 T23585 punct ",",µM
R17978 T23587 T23585 appos Gö-6976,µM
R17979 T23588 T23587 punct :,Gö-6976
R1798 T2084 T2083 pobj PKC,of
R17980 T23589 T23590 nummod 5,µM
R17981 T23590 T23587 conj µM,Gö-6976
R17982 T23591 T23590 punct ",",µM
R17983 T23592 T23590 conj BAPTA/AM,µM
R17984 T23593 T23592 punct :,BAPTA/AM
R17985 T23594 T23595 nummod 2.5,µM
R17986 T23595 T23592 appos µM,BAPTA/AM
R17987 T23596 T23592 punct ),BAPTA/AM
R17988 T23597 T23592 prep for,BAPTA/AM
R17989 T23598 T23599 nummod 30,minutes
R17990 T23599 T23597 pobj minutes,for
R17991 T23600 T23587 npadvmod prior,Gö-6976
R17992 T23601 T23600 prep to,prior
R17993 T23602 T23603 det the,addition
R17994 T23603 T23601 pobj addition,to
R17995 T23604 T23603 prep of,addition
R17996 T23605 T23604 pobj M-CSF,of
R17997 T23606 T23575 punct .,pre-incubated
R17998 T23607 T23614 prep As,incubated
R17999 T23608 T23609 det a,control
R180 T212 T215 amod M-CSF-induced,activation
R18000 T23609 T23607 pobj control,As
R18001 T23610 T23614 punct ",",incubated
R18002 T23611 T23612 amod untreated,cells
R18003 T23612 T23614 nsubjpass cells,incubated
R18004 T23613 T23614 auxpass were,incubated
R18005 T23614 T23614 ROOT incubated,incubated
R18006 T23615 T23614 prep with,incubated
R18007 T23616 T23617 compound dimethyl,sulfoxide
R18008 T23617 T23615 pobj sulfoxide,with
R18009 T23618 T23617 punct (,sulfoxide
R18010 T23619 T23617 appos DMSO,sulfoxide
R18011 T23620 T23617 punct ),sulfoxide
R18012 T23621 T23614 punct .,incubated
R18013 T23622 T23623 compound Cell,lysates
R18014 T23623 T23625 nsubjpass lysates,resolved
R18015 T23624 T23625 auxpass were,resolved
R18016 T23625 T23625 ROOT resolved,resolved
R18017 T23626 T23625 agent by,resolved
R18018 T23627 T23626 pobj SDS-PAGE,by
R18019 T23628 T23625 cc and,resolved
R18020 T23629 T23625 conj immunoblotted,resolved
R18021 T23630 T23629 prep with,immunoblotted
R18022 T23631 T23630 pobj antibody,with
R18023 T23632 T23630 pcomp recognizing,with
R18024 T23633 T23636 det the,form
R18025 T23634 T23636 amod active,form
R18026 T23635 T23636 amod cleaved,form
R18027 T23636 T23632 dobj form,recognizing
R18028 T23637 T23636 prep of,form
R18029 T23638 T23637 pobj caspase-3,of
R18030 T23639 T23625 punct .,resolved
R18031 T23640 T23641 det The,blots
R18032 T23641 T23643 nsubjpass blots,reblotted
R18033 T23642 T23643 auxpass were,reblotted
R18034 T23643 T23643 ROOT reblotted,reblotted
R18035 T23644 T23643 prep with,reblotted
R18036 T23645 T23644 pobj β-actin,with
R18037 T23646 T23647 nsubj that,served
R18038 T23647 T23643 conj served,reblotted
R18039 T23648 T23647 prep as,served
R1804 T2085 T2082 prep in,role
R18040 T23649 T23651 det a,control
R18041 T23650 T23651 compound loading,control
R18042 T23651 T23648 pobj control,as
R18043 T23652 T23643 punct .,reblotted
R18044 T23653 T23654 det The,ratio
R18045 T23654 T23670 nsubjpass ratio,determined
R18046 T23655 T23654 prep of,ratio
R18047 T23656 T23658 amod active,bands
R18048 T23657 T23658 compound caspase-3,bands
R18049 T23658 T23655 pobj bands,of
R18050 T23659 T23658 punct (,bands
R18051 T23660 T23661 nummod 17,kD
R18052 T23661 T23658 appos kD,bands
R18053 T23662 T23661 cc and,kD
R18054 T23663 T23664 nummod 19,kD
R18055 T23664 T23661 conj kD,kD
R18056 T23665 T23664 punct ),kD
R18057 T23666 T23670 prep to,determined
R18058 T23667 T23668 compound β-actin,control
R18059 T23668 T23666 pobj control,to
R1806 T2086 T2087 det the,regulation
R18060 T23669 T23670 auxpass was,determined
R18061 T23670 T23670 ROOT determined,determined
R18062 T23671 T23670 agent by,determined
R18063 T23672 T23673 amod densitometry,analysis
R18064 T23673 T23671 pobj analysis,by
R18065 T23674 T23676 punct (,panel
R18066 T23675 T23676 amod bottom,panel
R18067 T23676 T23673 appos panel,analysis
R18068 T23677 T23670 punct ),determined
R18069 T23678 T23670 punct .,determined
R18070 T23679 T23680 nsubj Data,represents
R18071 T23680 T23680 ROOT represents,represents
R18072 T23681 T23684 det the,S.E.M
R18073 T23682 T23684 amod mean,S.E.M
R18074 T23683 T23684 compound ±,S.E.M
R18075 T23684 T23680 dobj S.E.M,represents
R18076 T23685 T23684 prep from,S.E.M
R18077 T23686 T23688 nummod two,donors
R18078 T23687 T23688 amod independent,donors
R18079 T23688 T23685 pobj donors,from
R18080 T23689 T23680 punct .,represents
R18081 T23690 T23693 punct (,Apoptosis
R18082 T23691 T23693 nmod B,Apoptosis
R18083 T23692 T23693 punct ),Apoptosis
R18084 T23693 T23700 nsubjpass Apoptosis,measured
R18085 T23694 T23693 prep of,Apoptosis
R18086 T23695 T23697 det the,MDMs
R18087 T23696 T23697 amod treated,MDMs
R18088 T23697 T23694 pobj MDMs,of
R18089 T23698 T23700 auxpass was,measured
R18090 T23699 T23700 advmod also,measured
R18091 T23700 T23700 ROOT measured,measured
R18092 T23701 T23700 agent by,measured
R18093 T23702 T23703 compound Annexin,V-FITC
R18094 T23703 T23701 pobj V-FITC,by
R18095 T23704 T23703 cc and,V-FITC
R18096 T23705 T23706 compound propidium,iodine
R18097 T23706 T23703 conj iodine,V-FITC
R18098 T23707 T23708 punct (,PI
R18099 T23708 T23706 appos PI,iodine
R181 T213 T215 nmod NF-κB,activation
R1810 T2087 T2085 pobj regulation,in
R18100 T23709 T23706 punct ),iodine
R18101 T23710 T23700 advcl staining,measured
R18102 T23711 T23700 cc and,measured
R18103 T23712 T23700 conj analyzed,measured
R18104 T23713 T23712 agent by,analyzed
R18105 T23714 T23715 compound flow,cytometry
R18106 T23715 T23713 pobj cytometry,by
R18107 T23716 T23700 punct .,measured
R18108 T23717 T23718 det The,percentage
R18109 T23718 T23735 nsubjpass percentage,set
R18110 T23719 T23718 prep of,percentage
R18111 T23720 T23721 amod surviving,cells
R18112 T23721 T23719 pobj cells,of
R18113 T23722 T23718 punct (,percentage
R18114 T23723 T23724 compound Annexin,V/PI
R18115 T23724 T23718 appos V/PI,percentage
R18116 T23725 T23724 amod negative,V/PI
R18117 T23726 T23718 punct ),percentage
R18118 T23727 T23718 prep for,percentage
R18119 T23728 T23729 det the,cells
R18120 T23729 T23727 pobj cells,for
R18121 T23730 T23729 acl treated,cells
R18122 T23731 T23730 prep with,treated
R18123 T23732 T23731 pobj vehicle/M-CSF,with
R18124 T23733 T23735 auxpass was,set
R18125 T23734 T23735 advmod arbitrarily,set
R18126 T23735 T23735 ROOT set,set
R18127 T23736 T23735 prep as,set
R18128 T23737 T23736 pobj 100,as
R18129 T23738 T23735 punct .,set
R18130 T23739 T23741 nsubj Data,represent
R18131 T23740 T23739 acl shown,Data
R18132 T23741 T23741 ROOT represent,represent
R18133 T23742 T23745 det the,S.E.M
R18134 T23743 T23745 amod mean,S.E.M
R18135 T23744 T23745 compound ±,S.E.M
R18136 T23745 T23741 dobj S.E.M,represent
R18137 T23746 T23741 prep from,represent
R18138 T23747 T23749 nummod three,experiments
R18139 T23748 T23749 amod independent,experiments
R18140 T23749 T23746 pobj experiments,from
R18141 T23750 T23741 punct .,represent
R18142 T23751 T23751 ROOT *,*
R18143 T23752 T23753 det The,p-values
R18144 T23753 T23756 nsubj p-values,compared
R18145 T23754 T23753 prep of,p-values
R18146 T23755 T23754 pobj inhibitors/M-CSF,of
R18147 T23756 T23756 ROOT compared,compared
R18148 T23757 T23756 prep to,compared
R18149 T23758 T23757 pobj vehicle/M-CSF,to
R1815 T2088 T2087 prep of,regulation
R18150 T23759 T23756 auxpass were,compared
R18151 T23760 T23761 compound ≤,0.05
R18152 T23761 T23759 attr 0.05,were
R18153 T23762 T23756 punct .,compared
R1817 T2089 T2090 amod NF-κB,activation
R1818 T2090 T2088 pobj activation,of
R182 T214 T215 amod transcriptional,activation
R1824 T2091 T2090 cc and,activation
R1826 T2092 T2094 amod M-CSF-induced,survival
R1827 T2093 T2094 compound monocyte,survival
R183 T215 T211 pobj activation,for
R1836 T2094 T2090 conj survival,activation
R18363 T24095 T24096 nsubj M-CSF,activates
R18364 T24096 T24096 ROOT activates,activates
R18365 T24097 T24096 dobj PKCα,activates
R18366 T24098 T24096 prep in,activates
R18367 T24099 T24100 amod human,MDMs
R18368 T24100 T24098 pobj MDMs,in
R18369 T24101 T24096 punct .,activates
R18370 T24102 T24116 nsubjpass MDMs,lysed
R18371 T24103 T24102 acl treated,MDMs
R18372 T24104 T24103 prep with,treated
R18373 T24105 T24104 pobj M-CSF,with
R18374 T24106 T24108 punct (,ng/ml
R18375 T24107 T24108 nummod 100,ng/ml
R18376 T24108 T24105 appos ng/ml,M-CSF
R18377 T24109 T24103 punct ),treated
R18378 T24110 T24103 prep for,treated
R18379 T24111 T24112 amod varying,amounts
R18380 T24112 T24110 pobj amounts,for
R18381 T24113 T24112 prep of,amounts
R18382 T24114 T24113 pobj time,of
R18383 T24115 T24116 auxpass were,lysed
R18384 T24116 T24116 ROOT lysed,lysed
R18385 T24117 T24116 cc and,lysed
R18386 T24118 T24116 conj immunoprecipitated,lysed
R18387 T24119 T24118 advcl using,immunoprecipitated
R18388 T24120 T24121 amod anti-PKC,antibody
R18389 T24121 T24119 dobj antibody,using
R18390 T24122 T24121 cc or,antibody
R18391 T24123 T24121 conj control,antibody
R18392 T24124 T24125 compound IgG,antibody
R18393 T24125 T24121 conj antibody,antibody
R18394 T24126 T24116 punct .,lysed
R18395 T24127 T24128 nummod One,half
R18396 T24128 T24133 nsubjpass half,used
R18397 T24129 T24128 prep of,half
R18398 T24130 T24131 det the,samples
R18399 T24131 T24129 pobj samples,of
R184 T216 T215 punct ",",activation
R1840 T2095 T2078 punct .,focused
R18400 T24132 T24133 auxpass was,used
R18401 T24133 T24133 ROOT used,used
R18402 T24134 T24135 aux to,analyze
R18403 T24135 T24133 xcomp analyze,used
R18404 T24136 T24138 compound PKC,activity
R18405 T24137 T24138 compound kinase,activity
R18406 T24138 T24135 dobj activity,analyze
R18407 T24139 T24135 advcl using,analyze
R18408 T24140 T24141 det a,fluorescein
R18409 T24141 T24139 dobj fluorescein,using
R18410 T24142 T24133 conj tagged,used
R18411 T24143 T24142 dobj peptide,tagged
R18412 T24144 T24142 cc and,tagged
R18413 T24145 T24142 conj visualized,tagged
R18414 T24146 T24145 agent by,visualized
R18415 T24147 T24149 amod agarose,electrophoresis
R18416 T24148 T24149 compound gel,electrophoresis
R18417 T24149 T24146 pobj electrophoresis,by
R18418 T24150 T24149 punct (,electrophoresis
R18419 T24151 T24152 amod top,panel
R18420 T24152 T24149 appos panel,electrophoresis
R18421 T24153 T24149 punct ),electrophoresis
R18422 T24154 T24142 punct ",",tagged
R18423 T24155 T24160 mark while,subjected
R18424 T24156 T24158 det the,half
R18425 T24157 T24158 amod other,half
R18426 T24158 T24160 nsubjpass half,subjected
R18427 T24159 T24160 auxpass was,subjected
R18428 T24160 T24142 advcl subjected,tagged
R18429 T24161 T24160 prep to,subjected
R18430 T24162 T24164 amod Western,analysis
R18431 T24163 T24164 compound blot,analysis
R18432 T24164 T24161 pobj analysis,to
R18433 T24165 T24166 aux to,confirm
R18434 T24166 T24160 xcomp confirm,subjected
R18435 T24167 T24168 amod equal,amounts
R18436 T24168 T24166 dobj amounts,confirm
R18437 T24169 T24168 prep of,amounts
R18438 T24170 T24169 pobj PKCα,of
R18439 T24171 T24172 auxpass were,immunoprecipitated
R18440 T24172 T24142 conj immunoprecipitated,tagged
R18441 T24173 T24172 prep from,immunoprecipitated
R18442 T24174 T24175 det each,sample
R18443 T24175 T24173 pobj sample,from
R18444 T24176 T24178 punct (,panel
R18445 T24177 T24178 amod middle,panel
R18446 T24178 T24175 appos panel,sample
R18447 T24179 T24172 punct ),immunoprecipitated
R18448 T24180 T24133 punct .,used
R18449 T24181 T24183 det The,assay
R1845 T2096 T2098 advmod Here,demonstrates
R18450 T24182 T24183 compound kinase,assay
R18451 T24183 T24185 nsubjpass assay,quantitated
R18452 T24184 T24185 auxpass was,quantitated
R18453 T24185 T24185 ROOT quantitated,quantitated
R18454 T24186 T24185 advcl using,quantitated
R18455 T24187 T24186 dobj Quantity,using
R18456 T24188 T24189 nummod One,software
R18457 T24189 T24195 nmod software,panel
R18458 T24190 T24191 punct (,Bio-Rad
R18459 T24191 T24189 appos Bio-Rad,software
R18460 T24192 T24189 punct ),software
R18461 T24193 T24195 punct (,panel
R18462 T24194 T24195 amod bottom,panel
R18463 T24195 T24187 appos panel,Quantity
R18464 T24196 T24187 punct ),Quantity
R18465 T24197 T24185 punct .,quantitated
R18466 T24198 T24199 nsubj Data,represents
R18467 T24199 T24199 ROOT represents,represents
R18468 T24200 T24203 det the,increase
R18469 T24201 T24202 amod average,fold
R18470 T24202 T24203 compound fold,increase
R18471 T24203 T24199 dobj increase,represents
R18472 T24204 T24203 prep of,increase
R18473 T24205 T24206 compound PKCα,activity
R18474 T24206 T24204 pobj activity,of
R18475 T24207 T24206 prep in,activity
R18476 T24208 T24209 amod non-stimulated,samples
R18477 T24209 T24207 pobj samples,in
R18478 T24210 T24199 prep compared,represents
R18479 T24211 T24210 prep to,compared
R1848 T2097 T2098 nsubj we,demonstrates
R18480 T24212 T24215 compound M-CSF-treated,S.E.M
R18481 T24213 T24214 compound MDM,±
R18482 T24214 T24215 compound ±,S.E.M
R18483 T24215 T24211 pobj S.E.M,to
R18484 T24216 T24210 prep for,compared
R18485 T24217 T24219 nummod three,experiments
R18486 T24218 T24219 amod independent,experiments
R18487 T24219 T24216 pobj experiments,for
R18488 T24220 T24199 punct .,represents
R18489 T24221 T24221 ROOT NS,NS
R18490 T24222 T24221 punct :,NS
R18491 T24223 T24221 acl non-stimulated,NS
R18492 T24224 T24221 punct .,NS
R18493 T24225 T24225 ROOT *,*
R18494 T24226 T24227 det The,p-values
R18495 T24227 T24230 nsubj p-values,stimulated
R18496 T24228 T24227 prep of,p-values
R18497 T24229 T24228 pobj M-CSF,of
R18498 T24230 T24230 ROOT stimulated,stimulated
R18499 T24231 T24230 prep compared,stimulated
R185 T217 T219 amod NF-κB-regulated,expression
R18500 T24232 T24231 prep to,compared
R18501 T24233 T24232 pobj non-stimulated,to
R18502 T24234 T24230 conj were,stimulated
R18503 T24235 T24236 compound ≤,0.05
R18504 T24236 T24234 attr 0.05,were
R18505 T24237 T24230 punct .,stimulated
R1853 T2098 T2098 ROOT demonstrates,demonstrates
R1859 T2099 T2101 mark that,upregulated
R186 T218 T219 compound gene,expression
R1861 T2100 T2101 nsubj M-CSF,upregulated
R1864 T2101 T2098 ccomp upregulated,demonstrates
R1865 T2102 T2104 det the,transcription
R18676 T24510 T24512 amod M-CSF-induced,activity
R18677 T24511 T24512 compound PKC,activity
R18678 T24512 T24515 nsubj activity,regulate
R18679 T24513 T24515 aux does,regulate
R1868 T2103 T2104 compound NF-κB,transcription
R18680 T24514 T24515 neg not,regulate
R18681 T24515 T24515 ROOT regulate,regulate
R18682 T24516 T24517 compound IκBα,degradation
R18683 T24517 T24515 dobj degradation,regulate
R18684 T24518 T24515 cc but,regulate
R18685 T24519 T24515 conj regulates,regulate
R18686 T24520 T24521 det the,phosphorylation
R18687 T24521 T24519 dobj phosphorylation,regulates
R18688 T24522 T24521 prep of,phosphorylation
R18689 T24523 T24524 compound NF-κB,p65
R18690 T24524 T24522 pobj p65,of
R18691 T24525 T24519 prep at,regulates
R18692 T24526 T24525 pobj Ser276,at
R18693 T24527 T24515 punct .,regulate
R18694 T24528 T24529 punct (,A
R18695 T24529 T24529 ROOT A,A
R18696 T24530 T24529 punct ),A
R18697 T24531 T24533 nsubjpass MDMs,pretreated
R18698 T24532 T24533 auxpass were,pretreated
R18699 T24533 T24533 ROOT pretreated,pretreated
R187 T219 T215 conj expression,activation
R18700 T24534 T24533 prep with,pretreated
R18701 T24535 T24534 pobj cycloheximide,with
R18702 T24536 T24535 punct (,cycloheximide
R18703 T24537 T24535 appos CHX,cycloheximide
R18704 T24538 T24535 punct ),cycloheximide
R18705 T24539 T24533 prep in,pretreated
R18706 T24540 T24541 det the,absence
R18707 T24541 T24539 pobj absence,in
R18708 T24542 T24541 cc or,absence
R18709 T24543 T24541 conj presence,absence
R1871 T2104 T2101 dobj transcription,upregulated
R18710 T24544 T24541 prep of,absence
R18711 T24545 T24544 pobj Ro-31-8220,of
R18712 T24546 T24533 prep for,pretreated
R18713 T24547 T24548 nummod 30,minutes
R18714 T24548 T24546 pobj minutes,for
R18715 T24549 T24533 advmod prior,pretreated
R18716 T24550 T24549 prep to,prior
R18717 T24551 T24552 compound M-CSF,stimulation
R18718 T24552 T24550 pobj stimulation,to
R18719 T24553 T24549 prep for,prior
R18720 T24554 T24556 det the,times
R18721 T24555 T24556 amod indicated,times
R18722 T24556 T24553 pobj times,for
R18723 T24557 T24533 punct .,pretreated
R18724 T24558 T24560 amod Whole,lysates
R18725 T24559 T24560 compound cell,lysates
R18726 T24560 T24562 nsubjpass lysates,subjected
R18727 T24561 T24562 auxpass were,subjected
R18728 T24562 T24562 ROOT subjected,subjected
R18729 T24563 T24562 prep to,subjected
R18730 T24564 T24565 amod Western,blotting
R18731 T24565 T24563 pobj blotting,to
R18732 T24566 T24562 prep with,subjected
R18733 T24567 T24568 amod anti-IκBα,antibody
R18734 T24568 T24566 pobj antibody,with
R18735 T24569 T24562 punct .,subjected
R18736 T24570 T24571 nsubj Data,are
R18737 T24571 T24571 ROOT are,are
R18738 T24572 T24571 attr representative,are
R18739 T24573 T24572 prep of,representative
R18740 T24574 T24576 nummod three,experiments
R18741 T24575 T24576 amod independent,experiments
R18742 T24576 T24573 pobj experiments,of
R18743 T24577 T24571 punct .,are
R18744 T24578 T24579 punct (,B
R18745 T24579 T24579 ROOT B,B
R18746 T24580 T24579 punct ),B
R18747 T24581 T24583 nsubjpass MDMs,pretreated
R18748 T24582 T24583 auxpass were,pretreated
R18749 T24583 T24583 ROOT pretreated,pretreated
R18750 T24584 T24583 prep with,pretreated
R18751 T24585 T24586 det either,vehicle
R18752 T24586 T24584 pobj vehicle,with
R18753 T24587 T24586 cc or,vehicle
R18754 T24588 T24586 conj Ro-31-8220,vehicle
R18755 T24589 T24583 prep for,pretreated
R18756 T24590 T24591 nummod 30,minutes
R18757 T24591 T24589 pobj minutes,for
R18758 T24592 T24591 prep before,minutes
R18759 T24593 T24594 det the,addition
R18760 T24594 T24592 pobj addition,before
R18761 T24595 T24594 prep of,addition
R18762 T24596 T24595 pobj M-CSF,of
R18763 T24597 T24583 prep for,pretreated
R18764 T24598 T24599 nummod 10,minutes
R18765 T24599 T24597 pobj minutes,for
R18766 T24600 T24583 punct .,pretreated
R18767 T24601 T24602 amod Whole,cell
R18768 T24602 T24603 compound cell,lysates
R18769 T24603 T24605 nsubjpass lysates,resolved
R18770 T24604 T24605 auxpass were,resolved
R18771 T24605 T24605 ROOT resolved,resolved
R18772 T24606 T24605 agent by,resolved
R18773 T24607 T24606 pobj SDS-PAGE,by
R18774 T24608 T24607 cc and,SDS-PAGE
R18775 T24609 T24607 conj phospho-Ser276,SDS-PAGE
R18776 T24610 T24609 cc or,phospho-Ser276
R18777 T24611 T24609 conj phospho-Ser536,phospho-Ser276
R18778 T24612 T24611 prep of,phospho-Ser536
R18779 T24613 T24612 pobj NF-κB,of
R18780 T24614 T24613 nummod p65,NF-κB
R18781 T24615 T24616 auxpass was,detected
R18782 T24616 T24605 conj detected,resolved
R18783 T24617 T24616 advcl using,detected
R18784 T24618 T24619 amod phospho-specific,antibodies
R18785 T24619 T24617 dobj antibodies,using
R18786 T24620 T24617 prep to,using
R18787 T24621 T24622 det either,residue
R18788 T24622 T24620 pobj residue,to
R18789 T24623 T24622 prep of,residue
R1879 T2105 T2104 cc and,transcription
R18790 T24624 T24625 compound NF-κB,p65
R18791 T24625 T24623 pobj p65,of
R18792 T24626 T24605 punct .,resolved
R18793 T24627 T24628 punct (,C
R18794 T24628 T24632 nmod C,lysates
R18795 T24629 T24628 punct ),C
R18796 T24630 T24631 amod Whole,cell
R18797 T24631 T24632 compound cell,lysates
R18798 T24632 T24651 nsubjpass lysates,subjected
R18799 T24633 T24632 prep of,lysates
R188 T220 T215 punct ",",activation
R18800 T24634 T24633 pobj RAW,of
R18801 T24635 T24634 nummod 264.7,RAW
R18802 T24636 T24632 conj cells,lysates
R18803 T24637 T24632 acl treated,lysates
R18804 T24638 T24637 prep with,treated
R18805 T24639 T24640 compound vehicle,control
R18806 T24640 T24638 pobj control,with
R18807 T24641 T24640 cc or,control
R18808 T24642 T24640 conj Ro-31-8220,control
R18809 T24643 T24637 prep in,treated
R18810 T24644 T24645 det the,absence
R18811 T24645 T24643 pobj absence,in
R18812 T24646 T24645 cc or,absence
R18813 T24647 T24645 conj presence,absence
R18814 T24648 T24645 prep of,absence
R18815 T24649 T24648 pobj M-CSF,of
R18816 T24650 T24651 auxpass were,subjected
R18817 T24651 T24651 ROOT subjected,subjected
R18818 T24652 T24651 prep to,subjected
R18819 T24653 T24655 amod Western,analysis
R18820 T24654 T24655 compound blot,analysis
R18821 T24655 T24652 pobj analysis,to
R18822 T24656 T24655 prep with,analysis
R18823 T24657 T24656 pobj phospho-Ser276,with
R18824 T24658 T24657 cc or,phospho-Ser276
R18825 T24659 T24660 compound phospho-Ser536,NF-κB
R18826 T24660 T24657 conj NF-κB,phospho-Ser276
R18827 T24661 T24662 nummod p65,antibodies
R18828 T24662 T24656 pobj antibodies,with
R18829 T24663 T24651 punct .,subjected
R18830 T24664 T24667 punct (,Cytosolic
R18831 T24665 T24667 nmod D,Cytosolic
R18832 T24666 T24667 punct ),Cytosolic
R18833 T24667 T24675 nsubjpass Cytosolic,obtained
R18834 T24668 T24667 cc and,Cytosolic
R18835 T24669 T24670 amod nuclear,fractions
R18836 T24670 T24667 conj fractions,Cytosolic
R18837 T24671 T24670 prep of,fractions
R18838 T24672 T24675 nsubjpass RAW,obtained
R18839 T24673 T24672 nummod 264.7,RAW
R18840 T24674 T24675 auxpass were,obtained
R18841 T24675 T24675 ROOT obtained,obtained
R18842 T24676 T24675 prep from,obtained
R18843 T24677 T24679 det the,cells
R18844 T24678 T24679 amod treated,cells
R18845 T24679 T24676 pobj cells,from
R18846 T24680 T24675 cc and,obtained
R18847 T24681 T24675 conj immunoblotted,obtained
R18848 T24682 T24681 prep for,immunoblotted
R18849 T24683 T24682 pobj phospho-NF-κB,for
R1885 T2106 T2107 compound cell,survival
R18850 T24684 T24675 punct .,obtained
R18851 T24685 T24686 det The,purity
R18852 T24686 T24694 nsubjpass purity,confirmed
R18853 T24687 T24686 prep of,purity
R18854 T24688 T24692 det the,fractions
R18855 T24689 T24692 amod cytosolic,fractions
R18856 T24690 T24689 cc and,cytosolic
R18857 T24691 T24689 conj nuclear,cytosolic
R18858 T24692 T24687 pobj fractions,of
R18859 T24693 T24694 auxpass was,confirmed
R18860 T24694 T24694 ROOT confirmed,confirmed
R18861 T24695 T24694 agent by,confirmed
R18862 T24696 T24695 pcomp immunoblotting,by
R18863 T24697 T24696 prep with,immunoblotting
R18864 T24698 T24697 pobj GAPDH,with
R18865 T24699 T24698 cc and,GAPDH
R18866 T24700 T24701 compound Lamin,B
R18867 T24701 T24698 conj B,GAPDH
R18868 T24702 T24696 punct ",",immunoblotting
R18869 T24703 T24696 advmod respectively,immunoblotting
R18870 T24704 T24694 punct .,confirmed
R18871 T24705 T24705 ROOT Shown,Shown
R18872 T24706 T24705 auxpass are,Shown
R18873 T24707 T24708 compound representative,blots
R18874 T24708 T24705 nsubjpass blots,Shown
R18875 T24709 T24708 prep from,blots
R18876 T24710 T24712 nummod three,experiments
R18877 T24711 T24712 amod independent,experiments
R18878 T24712 T24709 pobj experiments,from
R18879 T24713 T24705 punct .,Shown
R1889 T2107 T2104 conj survival,transcription
R189 T221 T224 nmod NF-κB,phosphorylation
R1893 T2108 T2104 prep in,transcription
R1894 T2109 T2112 amod human,macrophages
R1898 T2110 T2109 cc and,human
R190 T222 T224 nummod p65,phosphorylation
R1905 T2111 T2109 conj mouse,human
R191 T223 T224 compound Ser276,phosphorylation
R1911 T2112 T2108 pobj macrophages,in
R1913 T2113 T2098 punct .,demonstrates
R1916 T2114 T2115 det This,activity
R19177 T25121 T25125 nsubj Inhibition,reduces
R19178 T25122 T25121 prep of,Inhibition
R19179 T25123 T25124 compound M-CSF-induced,PKC
R19180 T25124 T25122 pobj PKC,of
R19181 T25125 T25125 ROOT reduces,reduces
R19182 T25126 T25127 amod NF-κB-regulated,genes
R19183 T25127 T25125 dobj genes,reduces
R19184 T25128 T25127 prep in,genes
R19185 T25129 T25130 preconj both,MDMs
R19186 T25130 T25128 pobj MDMs,in
R19187 T25131 T25130 cc and,MDMs
R19188 T25132 T25130 conj RAW,MDMs
R19189 T25133 T25134 nummod 264.7,cells
R1919 T2115 T2117 nsubjpass activity,reduced
R19190 T25134 T25130 conj cells,MDMs
R19191 T25135 T25125 punct .,reduces
R19192 T25136 T25136 ROOT MDMs,MDMs
R19193 T25137 T25138 punct (,A
R19194 T25138 T25136 appos A,MDMs
R19195 T25139 T25138 cc and,A
R19196 T25140 T25148 nmod C,cells
R19197 T25141 T25140 punct ),C
R19198 T25142 T25140 cc and,C
R19199 T25143 T25140 conj RAW,C
R192 T224 T215 conj phosphorylation,activation
R19200 T25144 T25143 nummod 264.7,RAW
R19201 T25145 T25146 punct (,B
R19202 T25146 T25143 appos B,RAW
R19203 T25147 T25143 punct ),RAW
R19204 T25148 T25150 nsubjpass cells,pretreated
R19205 T25149 T25150 auxpass were,pretreated
R19206 T25150 T25138 conj pretreated,A
R19207 T25151 T25150 prep with,pretreated
R19208 T25152 T25151 pobj Ro-31-8220,with
R19209 T25153 T25152 cc or,Ro-31-8220
R19210 T25154 T25155 amod solvent,control
R19211 T25155 T25156 compound control,DMSO
R19212 T25156 T25152 conj DMSO,Ro-31-8220
R19213 T25157 T25150 prep for,pretreated
R19214 T25158 T25159 nummod 30,minutes
R19215 T25159 T25157 pobj minutes,for
R19216 T25160 T25150 advmod prior,pretreated
R19217 T25161 T25160 prep to,prior
R19218 T25162 T25163 compound M-CSF,stimulation
R19219 T25163 T25161 pobj stimulation,to
R19220 T25164 T25160 prep for,prior
R19221 T25165 T25167 det the,times
R19222 T25166 T25167 amod indicated,times
R19223 T25167 T25164 pobj times,for
R19224 T25168 T25150 punct .,pretreated
R19225 T25169 T25170 amod Total,RNA
R19226 T25170 T25171 nsubj RNA,was
R19227 T25171 T25171 ROOT was,was
R19228 T25172 T25171 acomp isolated,was
R19229 T25173 T25172 cc and,isolated
R19230 T25174 T25172 conj converted,isolated
R19231 T25175 T25174 prep to,converted
R19232 T25176 T25175 pobj cDNA,to
R19233 T25177 T25171 punct .,was
R19234 T25178 T25180 amod Real-time,PCR
R19235 T25179 T25180 compound RT,PCR
R19236 T25180 T25182 nsubjpass PCR,performed
R19237 T25181 T25182 auxpass was,performed
R19238 T25182 T25182 ROOT performed,performed
R19239 T25183 T25182 advcl using,performed
R19240 T25184 T25183 dobj primers,using
R19241 T25185 T25183 prep for,using
R19242 T25186 T25185 pobj IκBα,for
R19243 T25187 T25186 punct ",",IκBα
R19244 T25188 T25186 conj BCL-xl,IκBα
R19245 T25189 T25188 cc or,BCL-xl
R19246 T25190 T25188 conj GAPDH,BCL-xl
R19247 T25191 T25182 punct .,performed
R19248 T25192 T25194 nsubjpass Data,expressed
R19249 T25193 T25194 auxpass are,expressed
R19250 T25194 T25194 ROOT expressed,expressed
R19251 T25195 T25194 prep as,expressed
R19252 T25196 T25197 amod relative,fold
R19253 T25197 T25198 compound fold,increase
R19254 T25198 T25195 pobj increase,as
R19255 T25199 T25198 prep of,increase
R19256 T25200 T25199 pobj IκBα,of
R19257 T25201 T25200 cc or,IκBα
R19258 T25202 T25200 conj BCL-xl,IκBα
R19259 T25203 T25204 compound gene,expression
R1926 T2116 T2117 auxpass was,reduced
R19260 T25204 T25198 conj expression,increase
R19261 T25205 T25204 prep upon,expression
R19262 T25206 T25205 pobj treatment,upon
R19263 T25207 T25204 prep over,expression
R19264 T25208 T25209 amod non-stimulated,cells
R19265 T25209 T25207 pobj cells,over
R19266 T25210 T25194 punct .,expressed
R19267 T25211 T25212 nsubj Data,represent
R19268 T25212 T25212 ROOT represent,represent
R19269 T25213 T25216 det the,S.E.M
R19270 T25214 T25216 amod mean,S.E.M
R19271 T25215 T25216 compound ±,S.E.M
R19272 T25216 T25212 dobj S.E.M,represent
R19273 T25217 T25212 prep for,represent
R19274 T25218 T25220 nummod three,experiments
R19275 T25219 T25220 amod independent,experiments
R19276 T25220 T25217 pobj experiments,for
R19277 T25221 T25212 punct .,represent
R193 T225 T224 punct ",",phosphorylation
R1930 T2117 T2117 ROOT reduced,reduced
R1933 T2118 T2117 agent by,reduced
R1936 T2119 T2120 det the,conventional
R194 T226 T224 cc and,phosphorylation
R1940 T2120 T2118 pobj conventional,by
R19429 T25475 T25476 nsubj PKCα,regulates
R19430 T25476 T25476 ROOT regulates,regulates
R19431 T25477 T25476 dobj phosphorylation,regulates
R19432 T25478 T25477 prep of,phosphorylation
R19433 T25479 T25480 compound NF-κB,p65
R19434 T25480 T25478 pobj p65,of
R19435 T25481 T25476 prep at,regulates
R19436 T25482 T25481 pobj Ser276,at
R19437 T25483 T25476 punct .,regulates
R19438 T25484 T25497 punct (,transfected
R19439 T25485 T25494 nmod A,cells
R1944 T2121 T2117 punct ",",reduced
R19440 T25486 T25485 punct ),A
R19441 T25487 T25485 conj MDM,A
R19442 T25488 T25487 cc or,MDM
R19443 T25489 T25490 punct (,B
R19444 T25490 T25492 nmod B,RAW
R19445 T25491 T25492 punct ),RAW
R19446 T25492 T25487 conj RAW,MDM
R19447 T25493 T25494 nummod 264.7,cells
R19448 T25494 T25497 nsubjpass cells,transfected
R19449 T25495 T25497 auxpass were,transfected
R19450 T25496 T25497 advmod transiently,transfected
R19451 T25497 T25497 ROOT transfected,transfected
R19452 T25498 T25497 prep with,transfected
R19453 T25499 T25512 advcl pNF-κB-SEAP,construct
R19454 T25500 T25499 prep along,pNF-κB-SEAP
R19455 T25501 T25500 prep with,along
R19456 T25502 T25503 det either,WT-PKCα
R19457 T25503 T25501 pobj WT-PKCα,with
R19458 T25504 T25503 cc or,WT-PKCα
R19459 T25505 T25506 det the,kinase-deficient
R19460 T25506 T25512 nsubj kinase-deficient,construct
R19461 T25507 T25508 punct (,KD
R19462 T25508 T25506 appos KD,kinase-deficient
R19463 T25509 T25508 punct ),KD
R19464 T25510 T25511 punct -,PKCα
R19465 T25511 T25506 appos PKCα,kinase-deficient
R19466 T25512 T25497 advcl construct,transfected
R19467 T25513 T25512 prep at,construct
R19468 T25514 T25518 det a,ratio
R19469 T25515 T25518 nummod 1,ratio
R19470 T25516 T25518 nmod ∶,ratio
R19471 T25517 T25516 nummod 5,∶
R19472 T25518 T25513 pobj ratio,at
R19473 T25519 T25497 punct .,transfected
R19474 T25520 T25523 nsubjpass Cells,starved
R19475 T25521 T25523 auxpass were,starved
R19476 T25522 T25523 advmod serum,starved
R19477 T25523 T25523 ROOT starved,starved
R19478 T25524 T25523 cc and,starved
R19479 T25525 T25523 conj stimulated,starved
R1948 T2122 T2117 cc but,reduced
R19480 T25526 T25525 prep with,stimulated
R19481 T25527 T25526 pobj M-CSF,with
R19482 T25528 T25525 cc and,stimulated
R19483 T25529 T25531 advmod then,secretion
R19484 T25530 T25531 compound SEAP,secretion
R19485 T25531 T25536 nsubjpass secretion,measured
R19486 T25532 T25531 prep in,secretion
R19487 T25533 T25534 det the,medium
R19488 T25534 T25532 pobj medium,in
R19489 T25535 T25536 auxpass was,measured
R19490 T25536 T25525 conj measured,stimulated
R19491 T25537 T25536 punct .,measured
R19492 T25538 T25539 nsubj Data,is
R19493 T25539 T25539 ROOT is,is
R19494 T25540 T25539 prep from,is
R19495 T25541 T25540 prep of,from
R19496 T25542 T25544 nummod three,experiments
R19497 T25543 T25544 amod independent,experiments
R19498 T25544 T25541 pobj experiments,of
R19499 T25545 T25539 punct .,is
R195 T227 T228 compound macrophage,survival
R19500 T25546 T25547 det The,p-value
R19501 T25547 T25550 nsubj p-value,transfected
R19502 T25548 T25547 prep of,p-value
R19503 T25549 T25548 pobj cells,of
R19504 T25550 T25550 ROOT transfected,transfected
R19505 T25551 T25550 prep with,transfected
R19506 T25552 T25551 pobj KD,with
R19507 T25553 T25550 prep compared,transfected
R19508 T25554 T25553 prep to,compared
R19509 T25555 T25559 nsubj those,was
R1951 T2123 T2124 neg not,novel
R19510 T25556 T25555 amod transfected,those
R19511 T25557 T25556 prep with,transfected
R19512 T25558 T25557 pobj WT,with
R19513 T25559 T25559 ROOT was,was
R19514 T25560 T25559 attr 0.05,was
R19515 T25561 T25559 punct .,was
R19516 T25562 T25563 punct (,C
R19517 T25563 T25563 ROOT C,C
R19518 T25564 T25563 punct ),C
R19519 T25565 T25567 nsubjpass MDMs,removed
R19520 T25566 T25567 auxpass were,removed
R19521 T25567 T25567 ROOT removed,removed
R19522 T25568 T25567 prep from,removed
R19523 T25569 T25570 det the,plate
R19524 T25570 T25568 pobj plate,from
R19525 T25571 T25567 advcl using,removed
R19526 T25572 T25571 dobj accutase,using
R19527 T25573 T25572 cc and,accutase
R19528 T25574 T25572 conj apoptosis,accutase
R19529 T25575 T25572 prep of,accutase
R19530 T25576 T25575 pobj MDMs,of
R19531 T25577 T25578 auxpass was,measured
R19532 T25578 T25567 conj measured,removed
R19533 T25579 T25578 agent by,measured
R19534 T25580 T25581 compound flow,cytometry
R19535 T25581 T25579 pobj cytometry,by
R19536 T25582 T25578 advcl using,measured
R19537 T25583 T25584 compound Annexin,V-FITC
R19538 T25584 T25582 dobj V-FITC,using
R19539 T25585 T25584 cc and,V-FITC
R1954 T2124 T2117 conj novel,reduced
R19540 T25586 T25587 compound propidium,iodine
R19541 T25587 T25584 conj iodine,V-FITC
R19542 T25588 T25587 punct (,iodine
R19543 T25589 T25587 appos PI,iodine
R19544 T25590 T25587 punct ),iodine
R19545 T25591 T25567 punct .,removed
R19546 T25592 T25597 punct (,lysates
R19547 T25593 T25597 dep D,lysates
R19548 T25594 T25595 punct ),Whole
R19549 T25595 T25596 amod Whole,cell
R1955 T2125 T2124 punct ",",novel
R19550 T25596 T25597 nsubj cell,lysates
R19551 T25597 T25597 ROOT lysates,lysates
R19552 T25598 T25597 prep from,lysates
R19553 T25599 T25601 det the,RAW
R19554 T25600 T25601 amod transfected,RAW
R19555 T25601 T25598 pobj RAW,from
R19556 T25602 T25603 nummod 264.7,cells
R19557 T25603 T25605 nsubjpass cells,subjected
R19558 T25604 T25605 auxpass were,subjected
R19559 T25605 T25597 ccomp subjected,lysates
R1956 T2126 T2127 compound PKC,inhibitors
R19560 T25606 T25605 prep to,subjected
R19561 T25607 T25609 amod Western,analysis
R19562 T25608 T25609 compound blot,analysis
R19563 T25609 T25606 pobj analysis,to
R19564 T25610 T25609 prep with,analysis
R19565 T25611 T25610 pobj phospho-Ser276,with
R19566 T25612 T25611 cc or,phospho-Ser276
R19567 T25613 T25614 compound phospho-Ser536,NF-κB
R19568 T25614 T25611 conj NF-κB,phospho-Ser276
R19569 T25615 T25616 nummod p65,antibodies
R1957 T2127 T2124 appos inhibitors,novel
R19570 T25616 T25609 appos antibodies,analysis
R19571 T25617 T25597 punct .,lysates
R19572 T25618 T25620 nsubjpass Blots,immunoblotted
R19573 T25619 T25620 auxpass were,immunoblotted
R19574 T25620 T25620 ROOT immunoblotted,immunoblotted
R19575 T25621 T25620 prep with,immunoblotted
R19576 T25622 T25621 pobj PKCα,with
R19577 T25623 T25624 aux to,determine
R19578 T25624 T25620 advcl determine,immunoblotted
R19579 T25625 T25627 amod equal,expression
R19580 T25626 T25627 compound protein,expression
R19581 T25627 T25624 dobj expression,determine
R19582 T25628 T25627 prep for,expression
R19583 T25629 T25631 det the,constructs
R19584 T25630 T25631 compound PKCα,constructs
R19585 T25631 T25628 pobj constructs,for
R19586 T25632 T25620 punct .,immunoblotted
R19587 T25633 T25634 nsubj β-actin,served
R19588 T25634 T25634 ROOT served,served
R19589 T25635 T25634 prep as,served
R19590 T25636 T25638 det a,control
R19591 T25637 T25638 compound loading,control
R19592 T25638 T25635 pobj control,as
R19593 T25639 T25634 punct .,served
R19594 T25640 T25640 ROOT Shown,Shown
R19595 T25641 T25640 auxpass is,Shown
R19596 T25642 T25644 det a,blot
R19597 T25643 T25644 amod representative,blot
R19598 T25644 T25640 nsubjpass blot,Shown
R19599 T25645 T25644 prep from,blot
R196 T228 T224 conj survival,phosphorylation
R1960 T2128 T2127 punct ",",inhibitors
R19600 T25646 T25648 nummod three,experiments
R19601 T25647 T25648 amod independent,experiments
R19602 T25648 T25645 pobj experiments,from
R19603 T25649 T25640 punct .,Shown
R19604 T25650 T25653 punct (,MDM
R19605 T25651 T25653 nmod E,MDM
R19606 T25652 T25653 punct ),MDM
R19607 T25653 T25660 nmod MDM,cells
R19608 T25654 T25653 cc or,MDM
R19609 T25655 T25656 punct (,F
R19610 T25656 T25653 conj F,MDM
R19611 T25657 T25656 punct ),F
R19612 T25658 T25653 conj RAW,MDM
R19613 T25659 T25660 nummod 264.7,cells
R19614 T25660 T25663 nsubjpass cells,transfected
R19615 T25661 T25663 auxpass were,transfected
R19616 T25662 T25663 advmod transiently,transfected
R19617 T25663 T25663 ROOT transfected,transfected
R19618 T25664 T25663 prep with,transfected
R19619 T25665 T25666 det a,pNF-κB-SEAP
R19620 T25666 T25664 pobj pNF-κB-SEAP,with
R19621 T25667 T25666 prep along,pNF-κB-SEAP
R19622 T25668 T25667 prep with,along
R19623 T25669 T25673 det either,siRNA
R19624 T25670 T25673 nummod 100,siRNA
R19625 T25671 T25673 compound nM,siRNA
R19626 T25672 T25673 compound PKCα,siRNA
R19627 T25673 T25668 pobj siRNA,with
R19628 T25674 T25673 cc or,siRNA
R19629 T25675 T25676 compound control,siRNA
R1963 T2129 T2132 amod dominant,constructs
R19630 T25676 T25673 conj siRNA,siRNA
R19631 T25677 T25663 prep for,transfected
R19632 T25678 T25679 nummod 20-24,hours
R19633 T25679 T25677 pobj hours,for
R19634 T25680 T25663 punct .,transfected
R19635 T25681 T25684 nsubjpass Cells,starved
R19636 T25682 T25684 auxpass were,starved
R19637 T25683 T25684 advmod serum,starved
R19638 T25684 T25684 ROOT starved,starved
R19639 T25685 T25684 prep for,starved
R1964 T2130 T2132 amod negative,constructs
R19640 T25686 T25687 nummod 2-4,hours
R19641 T25687 T25685 pobj hours,for
R19642 T25688 T25684 cc and,starved
R19643 T25689 T25684 conj stimulated,starved
R19644 T25690 T25689 prep with,stimulated
R19645 T25691 T25693 nummod 100,M-CSF
R19646 T25692 T25693 amod ng/ml,M-CSF
R19647 T25693 T25690 pobj M-CSF,with
R19648 T25694 T25693 prep for,M-CSF
R19649 T25695 T25696 nummod 6,hours
R19650 T25696 T25694 pobj hours,for
R19651 T25697 T25693 prep for,M-CSF
R19652 T25698 T25697 pobj MDM,for
R19653 T25699 T25698 cc or,MDM
R19654 T25700 T25698 conj RAW,MDM
R19655 T25701 T25700 nummod 264.7,RAW
R19656 T25702 T25693 prep for,M-CSF
R19657 T25703 T25704 nummod 2,hours
R19658 T25704 T25702 pobj hours,for
R19659 T25705 T25693 cc and,M-CSF
R1966 T2131 T2132 compound PKCα,constructs
R19660 T25706 T25708 advmod then,secretion
R19661 T25707 T25708 compound SEAP,secretion
R19662 T25708 T25693 conj secretion,M-CSF
R19663 T25709 T25708 prep in,secretion
R19664 T25710 T25711 det the,medium
R19665 T25711 T25709 pobj medium,in
R19666 T25712 T25713 auxpass was,measured
R19667 T25713 T25684 conj measured,starved
R19668 T25714 T25684 punct .,starved
R19669 T25715 T25715 ROOT Shown,Shown
R19670 T25716 T25715 auxpass is,Shown
R19671 T25717 T25715 nsubjpass data,Shown
R19672 T25718 T25717 prep of,data
R19673 T25719 T25721 nummod three,experiments
R19674 T25720 T25721 amod independent,experiments
R19675 T25721 T25718 pobj experiments,of
R19676 T25722 T25715 punct .,Shown
R19677 T25723 T25724 punct (,G
R19678 T25724 T25724 ROOT G,G
R19679 T25725 T25724 punct ),G
R19680 T25726 T25728 nsubjpass MDMs,removed
R19681 T25727 T25728 auxpass were,removed
R19682 T25728 T25728 ROOT removed,removed
R19683 T25729 T25728 prep from,removed
R19684 T25730 T25731 det the,plate
R19685 T25731 T25729 pobj plate,from
R19686 T25732 T25728 advcl using,removed
R19687 T25733 T25732 dobj accutase,using
R19688 T25734 T25733 cc and,accutase
R19689 T25735 T25733 conj apoptosis,accutase
R19690 T25736 T25733 prep of,accutase
R19691 T25737 T25736 pobj MDMs,of
R19692 T25738 T25739 auxpass was,measured
R19693 T25739 T25728 conj measured,removed
R19694 T25740 T25739 agent by,measured
R19695 T25741 T25742 compound Annexin,V-FITC
R19696 T25742 T25740 pobj V-FITC,by
R19697 T25743 T25742 cc and,V-FITC
R19698 T25744 T25745 compound propidium,iodine
R19699 T25745 T25742 conj iodine,V-FITC
R197 T229 T201 punct .,identify
R19700 T25746 T25747 punct (,PI
R19701 T25747 T25745 appos PI,iodine
R19702 T25748 T25745 punct ),iodine
R19703 T25749 T25739 xcomp staining,measured
R19704 T25750 T25739 cc and,measured
R19705 T25751 T25739 conj analyzed,measured
R19706 T25752 T25751 agent by,analyzed
R19707 T25753 T25754 compound flow,cytometry
R19708 T25754 T25752 pobj cytometry,by
R19709 T25755 T25728 punct .,removed
R19710 T25756 T25761 punct (,lysates
R19711 T25757 T25760 compound H,cell
R19712 T25758 T25759 punct ),Whole
R19713 T25759 T25760 compound Whole,cell
R19714 T25760 T25761 nsubj cell,lysates
R19715 T25761 T25761 ROOT lysates,lysates
R19716 T25762 T25761 prep from,lysates
R19717 T25763 T25765 det the,MDM
R19718 T25764 T25765 amod transfected,MDM
R19719 T25765 T25769 nmod MDM,cells
R19720 T25766 T25765 cc and,MDM
R19721 T25767 T25765 conj RAW,MDM
R19722 T25768 T25769 nummod 264.7,cells
R19723 T25769 T25762 pobj cells,from
R19724 T25770 T25771 auxpass were,subjected
R19725 T25771 T25761 conj subjected,lysates
R19726 T25772 T25771 prep to,subjected
R19727 T25773 T25775 amod Western,analysis
R19728 T25774 T25775 compound blot,analysis
R19729 T25775 T25772 pobj analysis,to
R19730 T25776 T25771 prep with,subjected
R19731 T25777 T25778 compound PKCα,antibody
R19732 T25778 T25776 pobj antibody,with
R19733 T25779 T25761 punct .,lysates
R19734 T25780 T25781 nsubj β-actin,served
R19735 T25781 T25781 ROOT served,served
R19736 T25782 T25781 prep as,served
R19737 T25783 T25785 det a,control
R19738 T25784 T25785 compound loading,control
R19739 T25785 T25782 pobj control,as
R19740 T25786 T25781 punct .,served
R19741 T25787 T25787 ROOT Shown,Shown
R19742 T25788 T25787 auxpass is,Shown
R19743 T25789 T25791 det a,blot
R19744 T25790 T25791 amod representative,blot
R19745 T25791 T25787 nsubjpass blot,Shown
R19746 T25792 T25791 prep from,blot
R19747 T25793 T25794 advmod at,least
R19748 T25794 T25795 advmod least,three
R19749 T25795 T25797 nummod three,experiments
R19750 T25796 T25797 amod independent,experiments
R19751 T25797 T25792 pobj experiments,from
R19752 T25798 T25787 punct .,Shown
R1977 T2132 T2127 conj constructs,inhibitors
R1978 T2133 T2132 cc or,constructs
R198 T230 T233 advmod Lastly,find
R1984 T2134 T2135 compound PKCα,siRNA
R199 T231 T233 punct ",",find
R200 T232 T233 nsubj we,find
R201 T233 T233 ROOT find,find
R20141 T26362 T26364 compound NF-κB,Ser276
R20142 T26363 T26364 compound p65,Ser276
R20143 T26364 T26365 nsubj Ser276,is
R20144 T26365 T26365 ROOT is,is
R20145 T26366 T26365 acomp essential,is
R20146 T26367 T26366 prep in,essential
R20147 T26368 T26367 pcomp regulating,in
R20148 T26369 T26370 amod NF-κB,activity
R20149 T26370 T26368 dobj activity,regulating
R20150 T26371 T26365 punct .,is
R20151 T26372 T26373 punct (,A
R20152 T26373 T26373 ROOT A,A
R20153 T26374 T26373 punct ),A
R20154 T26375 T26378 amod Raw,line
R20155 T26376 T26378 nummod 264.7,line
R20156 T26377 T26378 compound cell,line
R20157 T26378 T26381 nsubjpass line,transfected
R20158 T26379 T26381 auxpass was,transfected
R20159 T26380 T26381 advmod transiently,transfected
R20160 T26381 T26381 ROOT transfected,transfected
R20161 T26382 T26381 prep with,transfected
R20162 T26383 T26390 amod pNF-κB-SEAP,encoding
R20163 T26384 T26383 prep along,pNF-κB-SEAP
R20164 T26385 T26384 prep with,along
R20165 T26386 T26387 amod empty,vector
R20166 T26387 T26385 pobj vector,with
R20167 T26388 T26387 cc or,vector
R20168 T26389 T26387 conj plasmid,vector
R20169 T26390 T26382 pobj encoding,with
R20170 T26391 T26392 det either,NF-κB
R20171 T26392 T26390 appos NF-κB,encoding
R20172 T26393 T26394 nummod p65,WT
R20173 T26394 T26392 conj WT,NF-κB
R20174 T26395 T26394 cc or,WT
R20175 T26396 T26394 conj NF-κB,WT
R20176 T26397 T26398 compound p65,276S/A
R20177 T26398 T26394 conj 276S/A,WT
R20178 T26399 T26381 punct .,transfected
R20179 T26400 T26401 det The,cells
R20180 T26401 T26403 nsubjpass cells,transfected
R20181 T26402 T26403 auxpass were,transfected
R20182 T26403 T26409 ccomp transfected,starved
R20183 T26404 T26403 prep for,transfected
R20184 T26405 T26406 nummod 18-24,hours
R20185 T26406 T26404 pobj hours,for
R20186 T26407 T26409 punct ",",starved
R20187 T26408 T26409 nsubj serum,starved
R20188 T26409 T26409 ROOT starved,starved
R20189 T26410 T26409 prep for,starved
R20190 T26411 T26412 nummod 4,hours
R20191 T26412 T26410 pobj hours,for
R20192 T26413 T26409 punct ",",starved
R20193 T26414 T26409 cc and,starved
R20194 T26415 T26416 advmod then,incubated
R20195 T26416 T26409 conj incubated,starved
R20196 T26417 T26416 prep with,incubated
R20197 T26418 T26419 nummod 10,µM
R20198 T26419 T26417 pobj µM,with
R20199 T26420 T26419 prep of,µM
R202 T234 T238 mark that,plays
R20200 T26421 T26420 pobj Ro-31-8220,of
R20201 T26422 T26416 prep for,incubated
R20202 T26423 T26424 nummod 30,minutes
R20203 T26424 T26422 pobj minutes,for
R20204 T26425 T26416 advmod prior,incubated
R20205 T26426 T26425 prep to,prior
R20206 T26427 T26426 pobj treatment,to
R20207 T26428 T26425 prep with,prior
R20208 T26429 T26430 nummod 100,ng/ml
R20209 T26430 T26428 pobj ng/ml,with
R20210 T26431 T26430 prep of,ng/ml
R20211 T26432 T26431 pobj M-CSF,of
R20212 T26433 T26430 prep for,ng/ml
R20213 T26434 T26435 nummod 2,hours
R20214 T26435 T26433 pobj hours,for
R20215 T26436 T26435 cc and,hours
R20216 T26437 T26438 compound SEAP,secretion
R20217 T26438 T26430 conj secretion,ng/ml
R20218 T26439 T26438 prep in,secretion
R20219 T26440 T26441 det the,medium
R20220 T26441 T26439 pobj medium,in
R20221 T26442 T26443 auxpass was,measured
R20222 T26443 T26416 conj measured,incubated
R20223 T26444 T26409 punct .,starved
R20224 T26445 T26450 punct (,−
R20225 T26446 T26449 compound B,p65
R20226 T26447 T26448 punct ),NF-κB
R20227 T26448 T26449 compound NF-κB,p65
R20228 T26449 T26450 nsubj p65,−
R20229 T26450 T26450 ROOT −,−
R20230 T26451 T26454 amod /,line
R20231 T26452 T26454 nummod −,line
R20232 T26453 T26454 compound cell,line
R20233 T26454 T26457 nsubjpass line,transfected
R20234 T26455 T26457 auxpass was,transfected
R20235 T26456 T26457 advmod transiently,transfected
R20236 T26457 T26450 ccomp transfected,−
R20237 T26458 T26457 prep with,transfected
R20238 T26459 T26466 amod pNF-κB-SEAP,encoding
R20239 T26460 T26459 prep along,pNF-κB-SEAP
R20240 T26461 T26460 prep with,along
R20241 T26462 T26463 amod empty,vector
R20242 T26463 T26461 pobj vector,with
R20243 T26464 T26463 cc or,vector
R20244 T26465 T26463 conj plasmid,vector
R20245 T26466 T26470 amod encoding,WT
R20246 T26467 T26470 preconj either,WT
R20247 T26468 T26470 compound NF-κB,WT
R20248 T26469 T26470 compound p65,WT
R20249 T26470 T26458 pobj WT,with
R20250 T26471 T26470 punct ",",WT
R20251 T26472 T26474 compound NF-κB,276S/A
R20252 T26473 T26474 compound p65,276S/A
R20253 T26474 T26470 conj 276S/A,WT
R20254 T26475 T26474 punct ",",276S/A
R20255 T26476 T26474 cc or,276S/A
R20256 T26477 T26474 conj NF-κB,276S/A
R20257 T26478 T26479 compound p65,536S/A
R20258 T26479 T26474 conj 536S/A,276S/A
R20259 T26480 T26450 punct .,−
R20260 T26481 T26482 det The,cells
R20261 T26482 T26483 nsubj cells,were
R20262 T26483 T26483 ROOT were,were
R20263 T26484 T26483 acomp cultured,were
R20264 T26485 T26484 prep for,cultured
R20265 T26486 T26487 nummod 24,hours
R20266 T26487 T26485 pobj hours,for
R20267 T26488 T26484 cc and,cultured
R20268 T26489 T26491 advmod then,starved
R20269 T26490 T26491 nsubj serum,starved
R20270 T26491 T26484 conj starved,cultured
R20271 T26492 T26491 prep for,starved
R20272 T26493 T26494 nummod 4,hours
R20273 T26494 T26492 pobj hours,for
R20274 T26495 T26483 punct .,were
R20275 T26496 T26499 nsubjpass Cells,incubated
R20276 T26497 T26499 auxpass were,incubated
R20277 T26498 T26499 advmod then,incubated
R20278 T26499 T26499 ROOT incubated,incubated
R20279 T26500 T26499 prep in,incubated
R20280 T26501 T26503 amod fresh,medium
R20281 T26502 T26503 compound DMEM,medium
R20282 T26503 T26500 pobj medium,in
R20283 T26504 T26499 prep for,incubated
R20284 T26505 T26506 nummod 2,hours
R20285 T26506 T26504 pobj hours,for
R20286 T26507 T26506 cc and,hours
R20287 T26508 T26509 compound SEAP,secretion
R20288 T26509 T26514 nsubjpass secretion,measured
R20289 T26510 T26509 prep in,secretion
R20290 T26511 T26512 det the,medium
R20291 T26512 T26510 pobj medium,in
R20292 T26513 T26514 auxpass was,measured
R20293 T26514 T26499 conj measured,incubated
R20294 T26515 T26514 punct .,measured
R20295 T26516 T26517 det The,results
R20296 T26517 T26519 nsubj results,are
R20297 T26518 T26517 acl shown,results
R20298 T26519 T26519 ROOT are,are
R20299 T26520 T26519 xcomp fold,are
R203 T235 T237 compound NF-κB,Ser276
R20300 T26521 T26520 dobj change,fold
R20301 T26522 T26520 prep over,fold
R20302 T26523 T26526 amod empty,pTAL-SEAP
R20303 T26524 T26526 compound vector,pTAL-SEAP
R20304 T26525 T26526 compound +,pTAL-SEAP
R20305 T26526 T26522 pobj pTAL-SEAP,over
R20306 T26527 T26519 punct .,are
R20307 T26528 T26533 punct (,−
R20308 T26529 T26532 compound C,p65
R20309 T26530 T26531 punct ),NF-κB
R20310 T26531 T26532 compound NF-κB,p65
R20311 T26532 T26533 nsubj p65,−
R20312 T26533 T26533 ROOT −,−
R20313 T26534 T26537 amod /,line
R20314 T26535 T26537 nummod −,line
R20315 T26536 T26537 compound cell,line
R20316 T26537 T26540 nsubjpass line,transfected
R20317 T26538 T26540 auxpass was,transfected
R20318 T26539 T26540 advmod transiently,transfected
R20319 T26540 T26533 ccomp transfected,−
R20320 T26541 T26540 prep with,transfected
R20321 T26542 T26541 pobj pNF-κB-SEAP,with
R20322 T26543 T26542 prep with,pNF-κB-SEAP
R20323 T26544 T26546 preconj either,vector
R20324 T26545 T26546 amod empty,vector
R20325 T26546 T26543 pobj vector,with
R20326 T26547 T26546 cc or,vector
R20327 T26548 T26546 conj plasmid,vector
R20328 T26549 T26546 conj encoding,vector
R20329 T26550 T26551 det either,NF-κB
R20330 T26551 T26549 dobj NF-κB,encoding
R20331 T26552 T26553 nummod p65,WT
R20332 T26553 T26551 conj WT,NF-κB
R20333 T26554 T26553 cc or,WT
R20334 T26555 T26553 conj NF-κB,WT
R20335 T26556 T26557 compound p65,276S/A
R20336 T26557 T26553 conj 276S/A,WT
R20337 T26558 T26533 punct .,−
R20338 T26559 T26560 det The,cells
R20339 T26560 T26562 nsubjpass cells,transfected
R20340 T26561 T26562 auxpass were,transfected
R20341 T26562 T26562 ROOT transfected,transfected
R20342 T26563 T26562 prep for,transfected
R20343 T26564 T26565 nummod 24,hours
R20344 T26565 T26563 pobj hours,for
R20345 T26566 T26565 cc and,hours
R20346 T26567 T26565 conj serum,hours
R20347 T26568 T26562 conj starved,transfected
R20348 T26569 T26568 prep for,starved
R20349 T26570 T26571 nummod 4,hours
R20350 T26571 T26569 pobj hours,for
R20351 T26572 T26568 punct ",",starved
R20352 T26573 T26568 cc and,starved
R20353 T26574 T26575 advmod then,incubated
R20354 T26575 T26568 conj incubated,starved
R20355 T26576 T26575 prep with,incubated
R20356 T26577 T26578 nummod 10,µM
R20357 T26578 T26576 pobj µM,with
R20358 T26579 T26578 prep of,µM
R20359 T26580 T26579 pobj Ro-31-8220,of
R20360 T26581 T26575 prep for,incubated
R20361 T26582 T26583 nummod 30,minutes
R20362 T26583 T26581 pobj minutes,for
R20363 T26584 T26575 advmod prior,incubated
R20364 T26585 T26584 prep to,prior
R20365 T26586 T26585 pobj treatment,to
R20366 T26587 T26584 prep with,prior
R20367 T26588 T26589 nummod 10,ng/ml
R20368 T26589 T26587 pobj ng/ml,with
R20369 T26590 T26589 prep of,ng/ml
R20370 T26591 T26590 pobj TNFα,of
R20371 T26592 T26562 punct .,transfected
R20372 T26593 T26594 det The,supernatant
R20373 T26594 T26596 nsubjpass supernatant,collected
R20374 T26595 T26596 auxpass were,collected
R20375 T26596 T26596 ROOT collected,collected
R20376 T26597 T26609 mark after,measured
R20377 T26598 T26599 nummod 2,hours
R20378 T26599 T26597 pobj hours,after
R20379 T26600 T26599 prep of,hours
R20380 T26601 T26600 pobj treatment,of
R20381 T26602 T26601 cc and,treatment
R20382 T26603 T26604 compound SEAP,secretion
R20383 T26604 T26601 conj secretion,treatment
R20384 T26605 T26599 prep in,hours
R20385 T26606 T26607 det the,medium
R20386 T26607 T26605 pobj medium,in
R20387 T26608 T26609 auxpass was,measured
R20388 T26609 T26596 advcl measured,collected
R20389 T26610 T26596 punct .,collected
R20390 T26611 T26612 det The,results
R20391 T26612 T26614 nsubj results,are
R20392 T26613 T26612 acl shown,results
R20393 T26614 T26614 ROOT are,are
R20394 T26615 T26616 det the,fold
R20395 T26616 T26614 attr fold,are
R20396 T26617 T26616 dobj change,fold
R20397 T26618 T26616 prep over,fold
R20398 T26619 T26622 amod empty,pNF-κB-SEAP
R20399 T26620 T26622 compound vector,pNF-κB-SEAP
R204 T236 T237 compound p65,Ser276
R20400 T26621 T26622 compound +,pNF-κB-SEAP
R20401 T26622 T26618 pobj pNF-κB-SEAP,over
R20402 T26623 T26614 punct .,are
R20403 T26624 T26626 nsubj Data,are
R20404 T26625 T26624 acl shown,Data
R20405 T26626 T26626 ROOT are,are
R20406 T26627 T26629 amod mean,S.E.M
R20407 T26628 T26629 compound ±,S.E.M
R20408 T26629 T26626 attr S.E.M,are
R20409 T26630 T26629 prep for,S.E.M
R20410 T26631 T26632 advmod at,least
R20411 T26632 T26633 advmod least,three
R20412 T26633 T26635 nummod three,experiments
R20413 T26634 T26635 amod independent,experiments
R20414 T26635 T26630 pobj experiments,for
R20415 T26636 T26635 acl performed,experiments
R20416 T26637 T26636 prep in,performed
R20417 T26638 T26637 pobj duplicate,in
R20418 T26639 T26626 punct .,are
R20476 T26848 T26849 amod Proposed,model
R20477 T26849 T26849 ROOT model,model
R20478 T26850 T26849 prep for,model
R20479 T26851 T26853 amod M-CSF-induced,survival
R20480 T26852 T26853 compound monocyte,survival
R20481 T26853 T26850 pobj survival,for
R20482 T26854 T26849 prep via,model
R20483 T26855 T26856 compound PKC,regulation
R20484 T26856 T26854 pobj regulation,via
R20485 T26857 T26849 prep by,model
R20486 T26858 T26857 pcomp activating,by
R20487 T26859 T26861 nsubj NF-κB,phosphorylation
R20488 T26860 T26861 nummod p65,phosphorylation
R20489 T26861 T26858 dobj phosphorylation,activating
R20490 T26862 T26861 prep at,phosphorylation
R20491 T26863 T26862 pobj Ser276,at
R20492 T26864 T26849 punct .,model
R205 T237 T238 nsubj Ser276,plays
R206 T238 T233 ccomp plays,find
R207 T239 T241 det an,role
R208 T240 T241 amod important,role
R209 T241 T238 dobj role,plays
R210 T242 T241 prep in,role
R211 T243 T247 amod basal,activation
R212 T244 T243 cc and,basal
R213 T245 T243 conj M-CSF-stimulated,basal
R214 T246 T247 compound NF-κB,activation
R215 T247 T242 pobj activation,in
R216 T248 T247 prep in,activation
R217 T249 T250 amod human,mononuclear
R218 T250 T251 compound mononuclear,phagocytes
R219 T251 T248 pobj phagocytes,in
R220 T252 T233 punct .,find
R26 T58 T59 nsubj M-CSF,Induces
R27 T59 T79 nsubj Induces,promotes
R28 T60 T61 compound Monocyte,Survival
R29 T61 T59 dobj Survival,Induces
R30 T62 T59 prep by,Induces
R31 T63 T64 compound Activating,NF-κB
R32 T64 T62 pobj NF-κB,by
R33 T65 T66 nummod p65,Phosphorylation
R34 T66 T59 conj Phosphorylation,Induces
R3429 T4674 T4675 nsubj M-CSF,Induces
R3430 T4675 T4675 ROOT Induces,Induces
R3431 T4676 T4678 compound NF-κB,Activity
R3432 T4677 T4678 compound Transcriptional,Activity
R3433 T4678 T4675 dobj Activity,Induces
R3434 T4679 T4678 prep in,Activity
R3435 T4680 T4681 compound Human,Monocyte
R3436 T4681 T4679 pobj Monocyte,in
R3437 T4682 T4683 compound Derived,Macrophages
R3438 T4683 T4678 conj Macrophages,Activity
R3439 T4684 T4685 punct (,MDMs
R3440 T4685 T4683 appos MDMs,Macrophages
R3441 T4686 T4685 punct ),MDMs
R3442 T4687 T4683 cc and,Macrophages
R3443 T4688 T4691 compound Mouse,Line
R3444 T4689 T4691 compound Macrophage,Line
R3445 T4690 T4691 compound Cell,Line
R3446 T4691 T4683 conj Line,Macrophages
R3447 T4692 T4691 punct ",",Line
R3448 T4693 T4691 conj RAW,Line
R3449 T4694 T4693 nummod 264.7,RAW
R3450 T4695 T4696 aux To,determine
R3451 T4696 T4675 advcl determine,Induces
R3452 T4697 T4699 mark if,induced
R3453 T4698 T4699 nsubj M-CSF,induced
R3454 T4699 T4708 advcl induced,performed
R3455 T4700 T4701 compound NF-κB,DNA
R3456 T4701 T4699 dobj DNA,induced
R3457 T4702 T4699 advcl binding,induced
R3458 T4703 T4702 prep in,binding
R3459 T4704 T4705 amod human,macrophages
R3460 T4705 T4703 pobj macrophages,in
R3461 T4706 T4708 punct ",",performed
R3462 T4707 T4708 nsubj we,performed
R3463 T4708 T4675 conj performed,Induces
R3464 T4709 T4710 compound EMSA,analysis
R3465 T4710 T4708 dobj analysis,performed
R3466 T4711 T4708 prep on,performed
R3467 T4712 T4713 amod nuclear,lysates
R3468 T4713 T4711 pobj lysates,on
R3469 T4714 T4713 prep from,lysates
R3470 T4715 T4716 amod M-CSF-treated,MDMs
R3471 T4716 T4714 pobj MDMs,from
R3472 T4717 T4708 punct .,performed
R3473 T4718 T4718 ROOT Similar,Similar
R3474 T4719 T4718 prep to,Similar
R3475 T4720 T4721 amod previous,reports
R3476 T4721 T4719 pobj reports,to
R3477 T4722 T4724 compound [,]
R3478 T4723 T4724 compound 11,]
R3479 T4724 T4718 appos ],Similar
R3480 T4725 T4724 punct ",",]
R3481 T4726 T4727 amod nuclear,NF-κB
R3482 T4727 T4724 appos NF-κB,]
R3483 T4728 T4729 advmod constitutively,bound
R3484 T4729 T4730 amod bound,DNA
R3485 T4730 T4724 conj DNA,]
R3486 T4731 T4730 prep in,DNA
R3487 T4732 T4733 amod non-stimulated,monocytes
R3488 T4733 T4731 pobj monocytes,in
R3489 T4734 T4735 punct (,Figure
R3490 T4735 T4733 parataxis Figure,monocytes
R3491 T4736 T4735 nummod 1A,Figure
R3492 T4737 T4735 punct ),Figure
R3493 T4738 T4718 punct .,Similar
R3494 T4739 T4745 advmod Interestingly,alter
R3495 T4740 T4745 punct ",",alter
R3496 T4741 T4745 advcl adding,alter
R3497 T4742 T4741 dobj M-CSF,adding
R3498 T4743 T4745 aux did,alter
R3499 T4744 T4745 neg not,alter
R35 T67 T66 prep at,Phosphorylation
R3500 T4745 T4745 ROOT alter,alter
R3501 T4746 T4747 compound NF-κB,DNA
R3502 T4747 T4745 dobj DNA,alter
R3503 T4748 T4747 acl binding,DNA
R3504 T4749 T4748 agent by,binding
R3505 T4750 T4749 pobj EMSA,by
R3506 T4751 T4745 punct .,alter
R3507 T4752 T4776 prep In,resulted
R3508 T4753 T4752 pobj contrast,In
R3509 T4754 T4776 punct ",",resulted
R3510 T4755 T4776 prep after,resulted
R3511 T4756 T4757 advmod transiently,transfecting
R3512 T4757 T4755 pcomp transfecting,after
R3513 T4758 T4759 amod human,MDMs
R3514 T4759 T4757 dobj MDMs,transfecting
R3515 T4760 T4757 prep with,transfecting
R3516 T4761 T4762 compound pNF-κB-SEAP,constructs
R3517 T4762 T4760 pobj constructs,with
R3518 T4763 T4762 acl containing,constructs
R3519 T4764 T4768 nummod four,sequences
R3520 T4765 T4768 amod NF-κB,sequences
R3521 T4766 T4768 amod consensus,sequences
R3522 T4767 T4768 amod binding,sequences
R3523 T4768 T4763 dobj sequences,containing
R3524 T4769 T4768 punct ",",sequences
R3525 T4770 T4771 compound M-CSF,treatment
R3526 T4771 T4776 nsubj treatment,resulted
R3527 T4772 T4771 prep of,treatment
R3528 T4773 T4775 det the,cells
R3529 T4774 T4775 amod transfected,cells
R3530 T4775 T4772 pobj cells,of
R3531 T4776 T4776 ROOT resulted,resulted
R3532 T4777 T4776 prep in,resulted
R3533 T4778 T4780 det a,increase
R3534 T4779 T4780 amod 2.3-fold,increase
R3535 T4780 T4777 pobj increase,in
R3536 T4781 T4780 prep in,increase
R3537 T4782 T4783 compound SEAP,release
R3538 T4783 T4781 pobj release,in
R3539 T4784 T4783 prep in,release
R3540 T4785 T4787 det the,media
R3541 T4786 T4787 compound culture,media
R3542 T4787 T4784 pobj media,in
R3543 T4788 T4776 prep compared,resulted
R3544 T4789 T4788 prep to,compared
R3545 T4790 T4789 pobj PBS,to
R3546 T4791 T4792 punct (,vehicle
R3547 T4792 T4790 appos vehicle,PBS
R3548 T4793 T4790 punct ),PBS
R3549 T4794 T4795 punct -,treated
R3550 T4795 T4797 amod treated,MDMs
R3551 T4796 T4797 amod transfected,MDMs
R3552 T4797 T4789 pobj MDMs,to
R3553 T4798 T4800 punct (,1B
R3554 T4799 T4800 compound Figure,1B
R3555 T4800 T4797 appos 1B,MDMs
R3556 T4801 T4800 punct ),1B
R3557 T4802 T4776 punct .,resulted
R3558 T4803 T4809 prep As,construct
R3559 T4804 T4805 det a,control
R3560 T4805 T4803 pobj control,As
R3561 T4806 T4809 punct ",",construct
R3562 T4807 T4808 det the,pTAL-SEAP
R3563 T4808 T4809 nsubj pTAL-SEAP,construct
R3564 T4809 T4809 ROOT construct,construct
R3565 T4810 T4809 xcomp lacking,construct
R3566 T4811 T4813 amod NF-κB,sites
R3567 T4812 T4813 amod binding,sites
R3568 T4813 T4815 nsubjpass sites,used
R3569 T4814 T4815 auxpass was,used
R3570 T4815 T4810 ccomp used,lacking
R3571 T4816 T4809 punct .,construct
R3572 T4817 T4822 nsubj Cells,construct
R3573 T4818 T4817 acl transfected,Cells
R3574 T4819 T4818 prep with,transfected
R3575 T4820 T4821 det the,pTAL-SEAP
R3576 T4821 T4819 pobj pTAL-SEAP,with
R3577 T4822 T4822 ROOT construct,construct
R3578 T4823 T4825 aux did,produce
R3579 T4824 T4825 neg not,produce
R3580 T4825 T4822 conj produce,construct
R3581 T4826 T4825 dobj SEAP,produce
R3582 T4827 T4825 prep in,produce
R3583 T4828 T4829 det the,absence
R3584 T4829 T4827 pobj absence,in
R3585 T4830 T4829 cc or,absence
R3586 T4831 T4829 conj presence,absence
R3587 T4832 T4829 prep of,absence
R3588 T4833 T4832 pobj M-CSF,of
R3589 T4834 T4836 punct (,1B
R3590 T4835 T4836 compound Figure,1B
R3591 T4836 T4833 appos 1B,M-CSF
R3592 T4837 T4833 punct ),M-CSF
R3593 T4838 T4822 punct .,construct
R3594 T4839 T4841 nsubj We,investigated
R3595 T4840 T4841 advmod next,investigated
R3596 T4841 T4841 ROOT investigated,investigated
R3597 T4842 T4844 mark whether,induced
R3598 T4843 T4844 nsubj M-CSF,induced
R3599 T4844 T4841 ccomp induced,investigated
R36 T68 T67 pobj Ser276,at
R3600 T4845 T4846 compound NF-κB,activity
R3601 T4846 T4844 dobj activity,induced
R3602 T4847 T4844 prep in,induced
R3603 T4848 T4852 det the,line
R3604 T4849 T4852 compound mouse,line
R3605 T4850 T4852 compound macrophage,line
R3606 T4851 T4852 compound cell,line
R3607 T4852 T4847 pobj line,in
R3608 T4853 T4852 punct ",",line
R3609 T4854 T4852 appos RAW,line
R3610 T4855 T4854 nummod 264.7,RAW
R3611 T4856 T4841 punct .,investigated
R3612 T4857 T4861 nsubjpass RAW,transfected
R3613 T4858 T4859 nummod 264.7,cells
R3614 T4859 T4861 nsubjpass cells,transfected
R3615 T4860 T4861 auxpass were,transfected
R3616 T4861 T4861 ROOT transfected,transfected
R3617 T4862 T4861 prep with,transfected
R3618 T4863 T4866 preconj either,reporter
R3619 T4864 T4866 det the,reporter
R3620 T4865 T4866 compound NF-κB-SEAP,reporter
R3621 T4866 T4870 nsubj reporter,construct
R3622 T4867 T4866 cc or,reporter
R3623 T4868 T4866 conj control,reporter
R3624 T4869 T4870 amod pTAL-SEAP,construct
R3625 T4870 T4870 ROOT construct,construct
R3626 T4871 T4870 punct .,construct
R3627 T4872 T4873 mark As,shown
R3628 T4873 T4884 advcl shown,increased
R3629 T4874 T4873 prep in,shown
R3630 T4875 T4874 pobj Figure,in
R3631 T4876 T4875 nummod 1C,Figure
R3632 T4877 T4875 punct ",",Figure
R3633 T4878 T4879 compound M-CSF,treatment
R3634 T4879 T4875 appos treatment,Figure
R3635 T4880 T4879 prep of,treatment
R3636 T4881 T4880 pobj RAW,of
R3637 T4882 T4883 nummod 264.7,cells
R3638 T4883 T4884 nsubj cells,increased
R3639 T4884 T4884 ROOT increased,increased
R3640 T4885 T4887 amod NF-κB,activity
R3641 T4886 T4887 compound reporter,activity
R3642 T4887 T4884 dobj activity,increased
R3643 T4888 T4884 prep by,increased
R3644 T4889 T4888 pobj 2.5-fold,by
R3645 T4890 T4884 prep over,increased
R3646 T4891 T4890 pobj that,over
R3647 T4892 T4891 prep of,that
R3648 T4893 T4894 amod non-treated,cells
R3649 T4894 T4892 pobj cells,of
R3650 T4895 T4884 punct .,increased
R3651 T4896 T4900 advmod Together,demonstrate
R3652 T4897 T4900 punct ",",demonstrate
R3653 T4898 T4899 poss our,data
R3654 T4899 T4900 nsubj data,demonstrate
R3655 T4900 T4900 ROOT demonstrate,demonstrate
R3656 T4901 T4903 mark that,induced
R3657 T4902 T4903 nsubj M-CSF,induced
R3658 T4903 T4900 ccomp induced,demonstrate
R3659 T4904 T4906 compound NF-κB,activity
R3660 T4905 T4906 compound transcriptional,activity
R3661 T4906 T4903 dobj activity,induced
R3662 T4907 T4906 prep in,activity
R3663 T4908 T4907 pobj macrophages,in
R3664 T4909 T4900 punct .,demonstrate
R37 T69 T59 prep via,Induces
R38 T70 T71 compound Protein,Kinase
R39 T71 T73 compound Kinase,Macrophage
R3934 T5344 T5345 compound PKC,Inhibition
R3935 T5345 T5346 nsubj Inhibition,Reduces
R3936 T5346 T5346 ROOT Reduces,Reduces
R3937 T5347 T5348 compound NF-κB,Activity
R3938 T5348 T5346 dobj Activity,Reduces
R3939 T5349 T5348 prep in,Activity
R3940 T5350 T5351 compound Human,MDMs
R3941 T5351 T5349 pobj MDMs,in
R3942 T5352 T5351 cc and,MDMs
R3943 T5353 T5351 conj RAW,MDMs
R3944 T5354 T5355 nummod 264.7,Cells
R3945 T5355 T5348 conj Cells,Activity
R3946 T5356 T5359 mark Since,activated
R3947 T5357 T5359 nsubjpass NF-κB,activated
R3948 T5358 T5359 auxpass is,activated
R3949 T5359 T5372 advcl activated,determined
R3950 T5360 T5359 agent by,activated
R3951 T5361 T5360 pobj PKC,by
R3952 T5362 T5359 prep in,activated
R3953 T5363 T5365 amod several,types
R3954 T5364 T5365 compound cell,types
R3955 T5365 T5362 pobj types,in
R3956 T5366 T5368 nmod [,]
R3957 T5367 T5368 nummod 36,]
R3958 T5368 T5365 appos ],types
R3959 T5369 T5372 punct ",",determined
R3960 T5370 T5372 nsubj we,determined
R3961 T5371 T5372 advmod next,determined
R3962 T5372 T5346 ccomp determined,Reduces
R3963 T5373 T5378 mark if,was
R3964 T5374 T5377 amod M-CSF-induced,activity
R3965 T5375 T5377 amod NF-κB,activity
R3966 T5376 T5377 compound transcriptional,activity
R3967 T5377 T5378 nsubj activity,was
R3968 T5378 T5372 advcl was,determined
R3969 T5379 T5378 acomp dependent,was
R3970 T5380 T5379 prep on,dependent
R3971 T5381 T5382 compound PKC,activation
R3972 T5382 T5380 pobj activation,on
R3973 T5383 T5378 cc and,was
R3974 T5384 T5395 mark if,was
R3975 T5385 T5395 nsubj calcium,was
R3976 T5386 T5385 punct ",",calcium
R3977 T5387 T5388 det a,co-factor
R3978 T5388 T5385 conj co-factor,calcium
R3979 T5389 T5388 prep for,co-factor
R3980 T5390 T5393 amod conventional,activation
R3981 T5391 T5393 compound PKC,activation
R3982 T5392 T5393 compound isoform,activation
R3983 T5393 T5389 pobj activation,for
R3984 T5394 T5395 punct ",",was
R3985 T5395 T5378 conj was,was
R3986 T5396 T5395 acomp important,was
R3987 T5397 T5346 punct .,Reduces
R3988 T5398 T5420 prep In,used
R3989 T5399 T5398 pobj addition,In
R3990 T5400 T5420 punct ",",used
R3991 T5401 T5420 prep because,used
R3992 T5402 T5401 pcomp of,because
R3993 T5403 T5404 det the,number
R3994 T5404 T5401 pobj number,because
R3995 T5405 T5404 prep of,number
R3996 T5406 T5407 amod conventional,PKCs
R3997 T5407 T5405 pobj PKCs,of
R3998 T5408 T5404 acl existing,number
R3999 T5409 T5408 prep within,existing
R40 T72 T73 compound C,Macrophage
R4000 T5410 T5411 amod mononuclear,cells
R4001 T5411 T5409 pobj cells,within
R4002 T5412 T5420 punct ",",used
R4003 T5413 T5414 amod pharmacological,inhibitors
R4004 T5414 T5420 nsubjpass inhibitors,used
R4005 T5415 T5414 prep of,inhibitors
R4006 T5416 T5418 compound PKC,activation
R4007 T5417 T5418 compound family,activation
R4008 T5418 T5415 pobj activation,of
R4009 T5419 T5420 auxpass was,used
R4010 T5420 T5420 ROOT used,used
R4011 T5421 T5422 aux to,determine
R4012 T5422 T5420 xcomp determine,used
R4013 T5423 T5424 det the,relationship
R4014 T5424 T5422 dobj relationship,determine
R4015 T5425 T5424 prep of,relationship
R4016 T5426 T5427 compound PKC,activation
R4017 T5427 T5425 pobj activation,of
R4018 T5428 T5429 aux to,M-CSF-induced
R4019 T5429 T5424 relcl M-CSF-induced,relationship
R4020 T5430 T5431 amod cellular,survival
R4021 T5431 T5429 dobj survival,M-CSF-induced
R4022 T5432 T5420 punct .,used
R4023 T5433 T5435 nsubjpass MDMs,transfected
R4024 T5434 T5435 auxpass were,transfected
R4025 T5435 T5435 ROOT transfected,transfected
R4026 T5436 T5435 prep with,transfected
R4027 T5437 T5439 det the,reporter
R4028 T5438 T5439 compound pNF-κB-SEAP,reporter
R4029 T5439 T5436 pobj reporter,with
R4030 T5440 T5435 cc and,transfected
R4031 T5441 T5442 advmod then,treated
R4032 T5442 T5435 conj treated,transfected
R4033 T5443 T5442 prep with,treated
R4034 T5444 T5447 det the,inhibitor
R4035 T5445 T5447 amod general,inhibitor
R4036 T5446 T5447 compound PKC,inhibitor
R4037 T5447 T5443 pobj inhibitor,with
R4038 T5448 T5449 punct ",",Ro-31-8220
R4039 T5449 T5442 dep Ro-31-8220,treated
R4040 T5450 T5449 punct ;,Ro-31-8220
R4041 T5451 T5457 det the,Gö-6976
R4042 T5452 T5457 amod conventional,Gö-6976
R4043 T5453 T5457 compound PKCα,Gö-6976
R4044 T5454 T5457 compound /,Gö-6976
R4045 T5455 T5457 compound β,Gö-6976
R4046 T5456 T5457 compound inhibitor,Gö-6976
R4047 T5457 T5449 conj Gö-6976,Ro-31-8220
R4048 T5458 T5457 punct ;,Gö-6976
R4049 T5459 T5457 cc or,Gö-6976
R4050 T5460 T5463 det the,blocker
R4051 T5461 T5463 amod intracellular,blocker
R4052 T5462 T5463 compound calcium,blocker
R4053 T5463 T5457 conj blocker,Gö-6976
R4054 T5464 T5463 appos BAPTA/AM,blocker
R4055 T5465 T5435 punct .,transfected
R4056 T5466 T5471 amod Ro-31-8220,activity
R4057 T5467 T5468 advmod significantly,suppressed
R4058 T5468 T5471 amod suppressed,activity
R4059 T5469 T5471 amod M-CSF-induced,activity
R4060 T5470 T5471 compound NF-κB,activity
R4061 T5471 T5472 nsubj activity,compared
R4062 T5472 T5472 ROOT compared,compared
R4063 T5473 T5472 prep to,compared
R4064 T5474 T5473 pobj cells,to
R4065 T5475 T5474 acl treated,cells
R4066 T5476 T5475 prep with,treated
R4067 T5477 T5476 pobj M-CSF,with
R4068 T5478 T5477 advmod alone,M-CSF
R4069 T5479 T5481 punct (,2A
R4070 T5480 T5481 compound Figure,2A
R4071 T5481 T5477 appos 2A,M-CSF
R4072 T5482 T5481 punct ),2A
R4073 T5483 T5472 punct .,compared
R4074 T5484 T5491 prep In,blocked
R4075 T5485 T5484 pobj addition,In
R4076 T5486 T5491 punct ",",blocked
R4077 T5487 T5491 nsubj Gö-6976,blocked
R4078 T5488 T5487 cc and,Gö-6976
R4079 T5489 T5487 conj BAPTA/AM,Gö-6976
R4080 T5490 T5491 advmod also,blocked
R4081 T5491 T5491 ROOT blocked,blocked
R4082 T5492 T5493 amod NF-κB,activity
R4083 T5493 T5491 dobj activity,blocked
R4084 T5494 T5491 prep in,blocked
R4085 T5495 T5496 amod M-CSF-treated,MDMs
R4086 T5496 T5494 pobj MDMs,in
R4087 T5497 T5491 punct .,blocked
R4088 T5498 T5501 compound Trypan,analysis
R4089 T5499 T5500 amod blue,exclusion
R4090 T5500 T5501 compound exclusion,analysis
R4091 T5501 T5504 nsubj analysis,indicate
R4092 T5502 T5504 aux did,indicate
R4093 T5503 T5504 neg not,indicate
R4094 T5504 T5504 ROOT indicate,indicate
R4095 T5505 T5506 compound cell,death
R4096 T5506 T5504 dobj death,indicate
R4097 T5507 T5504 advcl suggesting,indicate
R4098 T5508 T5511 mark that,was
R4099 T5509 T5510 det this,suppression
R41 T73 T75 nmod Macrophage,factor
R4100 T5510 T5511 nsubj suppression,was
R4101 T5511 T5507 ccomp was,suggesting
R4102 T5512 T5511 neg not,was
R4103 T5513 T5511 acomp due,was
R4104 T5514 T5513 xcomp to,due
R4105 T5515 T5516 amod non-specific,toxicity
R4106 T5516 T5514 pobj toxicity,to
R4107 T5517 T5504 punct .,indicate
R4108 T5518 T5519 det These,data
R4109 T5519 T5520 nsubj data,indicate
R4110 T5520 T5520 ROOT indicate,indicate
R4111 T5521 T5523 mark that,mediated
R4112 T5522 T5523 nsubj M-CSF,mediated
R4113 T5523 T5520 ccomp mediated,indicate
R4114 T5524 T5525 compound NF-κB,activation
R4115 T5525 T5523 dobj activation,mediated
R4116 T5526 T5523 prep through,mediated
R4117 T5527 T5530 amod calcium-dependent,activation
R4118 T5528 T5530 amod conventional,activation
R4119 T5529 T5530 compound PKC,activation
R4120 T5530 T5526 pobj activation,through
R4121 T5531 T5520 punct .,indicate
R4122 T5532 T5534 nsubj We,investigated
R4123 T5533 T5534 advmod next,investigated
R4124 T5534 T5534 ROOT investigated,investigated
R4125 T5535 T5538 mark whether,affected
R4126 T5536 T5537 compound PKC,inhibition
R4127 T5537 T5538 nsubj inhibition,affected
R4128 T5538 T5534 ccomp affected,investigated
R4129 T5539 T5540 amod NF-κB,activity
R4130 T5540 T5538 dobj activity,affected
R4131 T5541 T5538 prep in,affected
R4132 T5542 T5546 det the,line
R4133 T5543 T5546 compound mouse,line
R4134 T5544 T5546 compound macrophage,line
R4135 T5545 T5546 compound cell,line
R4136 T5546 T5541 pobj line,in
R4137 T5547 T5546 punct ",",line
R4138 T5548 T5546 appos RAW,line
R4139 T5549 T5548 nummod 264.7,RAW
R4140 T5550 T5534 punct .,investigated
R4141 T5551 T5556 amod Similar,inhibitors
R4142 T5552 T5551 prep to,Similar
R4143 T5553 T5552 pobj MDMs,to
R4144 T5554 T5553 punct ",",MDMs
R4145 T5555 T5556 nsubj PKC,inhibitors
R4146 T5556 T5556 ROOT inhibitors,inhibitors
R4147 T5557 T5556 dobj Ro-31-8220,inhibitors
R4148 T5558 T5557 punct ",",Ro-31-8220
R4149 T5559 T5557 conj Gö-6976,Ro-31-8220
R4150 T5560 T5559 cc and,Gö-6976
R4151 T5561 T5559 conj BAPTA/AM,Gö-6976
R4152 T5562 T5556 conj reduced,inhibitors
R4153 T5563 T5565 amod M-CSF-induced,activity
R4154 T5564 T5565 compound NF-κB,activity
R4155 T5565 T5562 dobj activity,reduced
R4156 T5566 T5562 prep in,reduced
R4157 T5567 T5569 det a,manner
R4158 T5568 T5569 amod dose-dependent,manner
R4159 T5569 T5566 pobj manner,in
R4160 T5570 T5569 prep in,manner
R4161 T5571 T5570 pobj RAW,in
R4162 T5572 T5573 nummod 264.7,macrophages
R4163 T5573 T5569 appos macrophages,manner
R4164 T5574 T5576 punct (,2B
R4165 T5575 T5576 compound Figure,2B
R4166 T5576 T5573 appos 2B,macrophages
R4167 T5577 T5573 punct ),macrophages
R4168 T5578 T5556 punct .,inhibitors
R4169 T5579 T5580 aux To,ensure
R4170 T5580 T5580 ROOT ensure,ensure
R4171 T5581 T5584 mark that,regulated
R4172 T5582 T5584 nsubj PKC,regulated
R4173 T5583 T5584 advmod specifically,regulated
R4174 T5584 T5580 ccomp regulated,ensure
R4175 T5585 T5586 compound NF-κB,p65
R4176 T5586 T5584 dobj p65,regulated
R4177 T5587 T5586 cc and,p65
R4178 T5588 T5593 neg not,member
R4179 T5589 T5593 det the,member
R4180 T5590 T5591 advmod closely,related
R4181 T5591 T5593 amod related,member
R4182 T5592 T5593 compound family,member
R4183 T5593 T5602 nsubj member,constructs
R4184 T5594 T5593 punct ",",member
R4185 T5595 T5593 appos c-Rel,member
R4186 T5596 T5598 punct ",",co-transfected
R4187 T5597 T5598 nsubj we,co-transfected
R4188 T5598 T5593 parataxis co-transfected,member
R4189 T5599 T5598 dobj c-Rel,co-transfected
R4190 T5600 T5599 cc and,c-Rel
R4191 T5601 T5599 conj NF-κB-SEAP,c-Rel
R4192 T5602 T5586 conj constructs,p65
R4193 T5603 T5602 prep into,constructs
R4194 T5604 T5608 det the,line
R4195 T5605 T5608 nmod Raw,line
R4196 T5606 T5605 nummod 264.7,Raw
R4197 T5607 T5608 compound cell,line
R4198 T5608 T5603 pobj line,into
R4199 T5609 T5602 cc and,constructs
R42 T74 T75 amod colony-stimulating,factor
R4200 T5610 T5612 amod measured,activity
R4201 T5611 T5612 amod NF-κB,activity
R4202 T5612 T5602 conj activity,constructs
R4203 T5613 T5612 prep in,activity
R4204 T5614 T5613 pobj response,in
R4205 T5615 T5614 prep to,response
R4206 T5616 T5615 pobj M-CSF,to
R4207 T5617 T5580 punct .,ensure
R4208 T5618 T5619 expl There,was
R4209 T5619 T5619 ROOT was,was
R4210 T5620 T5623 det no,activity
R4211 T5621 T5623 amod increased,activity
R4212 T5622 T5623 compound NF-κB,activity
R4213 T5623 T5619 attr activity,was
R4214 T5624 T5619 prep in,was
R4215 T5625 T5624 pobj cells,in
R4216 T5626 T5625 acl expressing,cells
R4217 T5627 T5626 dobj c-Rel,expressing
R4218 T5628 T5630 punct (,S1
R4219 T5629 T5630 compound Figure,S1
R4220 T5630 T5627 appos S1,c-Rel
R4221 T5631 T5630 punct ),S1
R4222 T5632 T5619 punct .,was
R4223 T5633 T5634 det These,observations
R4224 T5634 T5635 nsubj observations,indicate
R4225 T5635 T5635 ROOT indicate,indicate
R4226 T5636 T5638 mark that,functioned
R4227 T5637 T5638 nsubj PKC,functioned
R4228 T5638 T5635 ccomp functioned,indicate
R4229 T5639 T5638 advmod upstream,functioned
R4230 T5640 T5639 prep of,upstream
R4231 T5641 T5640 pobj NF-κB,of
R4232 T5642 T5641 nummod p65,NF-κB
R4233 T5643 T5638 prep in,functioned
R4234 T5644 T5643 pobj MDMs,in
R4235 T5645 T5644 cc and,MDMs
R4236 T5646 T5644 conj RAW,MDMs
R4237 T5647 T5648 nummod 264.7,cells
R4238 T5648 T5644 conj cells,MDMs
R4239 T5649 T5635 punct .,indicate
R43 T75 T69 pobj factor,via
R44 T76 T75 punct (,factor
R45 T77 T75 appos M-CSF,factor
R46 T78 T75 punct ),factor
R4607 T6258 T6291 nsubj NF-κB,hypothesized
R4608 T6259 T6258 cc and,NF-κB
R4609 T6260 T6258 conj PKC,NF-κB
R4610 T6261 T6262 punct (,s
R4611 T6262 T6260 appos s,PKC
R4612 T6263 T6262 punct ),s
R4613 T6264 T6291 advcl Mediate,hypothesized
R4614 T6265 T6266 compound Human,MDM
R4615 T6266 T6267 compound MDM,Survival
R4616 T6267 T6264 dobj Survival,Mediate
R4617 T6268 T6267 prep in,Survival
R4618 T6269 T6268 pobj Response,in
R4619 T6270 T6273 acl to,Since
R4620 T6271 T6272 compound M-CSF,Stimulation
R4621 T6272 T6270 pobj Stimulation,to
R4622 T6273 T6277 mark Since,are
R4623 T6274 T6277 nsubj PKC,are
R4624 T6275 T6274 cc and,PKC
R4625 T6276 T6274 conj NF-κB,PKC
R4626 T6277 T6291 advcl are,hypothesized
R4627 T6278 T6277 acomp critical,are
R4628 T6279 T6278 prep in,critical
R4629 T6280 T6281 compound cell,survival
R4630 T6281 T6279 pobj survival,in
R4631 T6282 T6284 nmod [,]
R4632 T6283 T6284 nummod 37,]
R4633 T6284 T6277 npadvmod ],are
R4634 T6285 T6284 punct ",",]
R4635 T6286 T6288 nmod [,]
R4636 T6287 T6288 nummod 38,]
R4637 T6288 T6284 conj ],]
R4638 T6289 T6291 punct ",",hypothesized
R4639 T6290 T6291 nsubj we,hypothesized
R4640 T6291 T6291 ROOT hypothesized,hypothesized
R4641 T6292 T6294 mark that,promoted
R4642 T6293 T6294 nsubj M-CSF,promoted
R4643 T6294 T6291 ccomp promoted,hypothesized
R4644 T6295 T6296 compound cell,survival
R4645 T6296 T6294 dobj survival,promoted
R4646 T6297 T6294 prep through,promoted
R4647 T6298 T6297 pobj PKC,through
R4648 T6299 T6294 prep in,promoted
R4649 T6300 T6301 amod human,MDMs
R4650 T6301 T6299 pobj MDMs,in
R4651 T6302 T6291 punct .,hypothesized
R4652 T6303 T6304 aux To,detect
R4653 T6304 T6319 advcl detect,analyzed
R4654 T6305 T6304 dobj apoptosis,detect
R4655 T6306 T6305 punct ",",apoptosis
R4656 T6307 T6308 det the,expression
R4657 T6308 T6305 appos expression,apoptosis
R4658 T6309 T6308 prep of,expression
R4659 T6310 T6311 amod cleaved,caspase-3
R4660 T6311 T6309 pobj caspase-3,of
R4661 T6312 T6311 punct ",",caspase-3
R4662 T6313 T6314 det a,marker
R4663 T6314 T6311 appos marker,caspase-3
R4664 T6315 T6314 prep of,marker
R4665 T6316 T6315 pobj apoptosis,of
R4666 T6317 T6319 punct ",",analyzed
R4667 T6318 T6319 auxpass was,analyzed
R4668 T6319 T6319 ROOT analyzed,analyzed
R4669 T6320 T6319 prep in,analyzed
R4670 T6321 T6322 det the,presence
R4671 T6322 T6320 pobj presence,in
R4672 T6323 T6322 cc or,presence
R4673 T6324 T6322 conj absence,presence
R4674 T6325 T6324 prep of,absence
R4675 T6326 T6329 nmod M-CSF,inhibitors
R4676 T6327 T6326 cc and,M-CSF
R4677 T6328 T6326 conj PKC,M-CSF
R4678 T6329 T6325 pobj inhibitors,of
R4679 T6330 T6319 punct .,analyzed
R4680 T6331 T6353 prep In,elevated
R4681 T6332 T6331 pobj MDMs,In
R4682 T6333 T6332 acl pretreated,MDMs
R4683 T6334 T6333 prep with,pretreated
R4684 T6335 T6336 compound PKC,inhibitors
R4685 T6336 T6334 pobj inhibitors,with
R4686 T6337 T6336 punct (,inhibitors
R4687 T6338 T6336 appos Ro-31-8220,inhibitors
R4688 T6339 T6338 punct ",",Ro-31-8220
R4689 T6340 T6338 conj Gö-6976,Ro-31-8220
R4690 T6341 T6340 punct ",",Gö-6976
R4691 T6342 T6340 cc or,Gö-6976
R4692 T6343 T6340 conj BAPTA/AM,Gö-6976
R4693 T6344 T6343 punct ),BAPTA/AM
R4694 T6345 T6333 cc and,pretreated
R4695 T6346 T6333 conj stimulated,pretreated
R4696 T6347 T6346 prep with,stimulated
R4697 T6348 T6347 pobj M-CSF,with
R4698 T6349 T6350 punct ",",cleaved
R4699 T6350 T6351 compound cleaved,caspase-3
R47 T79 T79 ROOT promotes,promotes
R4700 T6351 T6353 nsubjpass caspase-3,elevated
R4701 T6352 T6353 auxpass was,elevated
R4702 T6353 T6353 ROOT elevated,elevated
R4703 T6354 T6353 prep to,elevated
R4704 T6355 T6354 pobj levels,to
R4705 T6356 T6355 prep of,levels
R4706 T6357 T6356 pobj cells,of
R4707 T6358 T6357 acl treated,cells
R4708 T6359 T6358 prep with,treated
R4709 T6360 T6359 pobj vehicle,with
R4710 T6361 T6360 advmod alone,vehicle
R4711 T6362 T6364 punct (,3A
R4712 T6363 T6364 compound Figure,3A
R4713 T6364 T6360 appos 3A,vehicle
R4714 T6365 T6353 punct ),elevated
R4715 T6366 T6353 punct .,elevated
R4716 T6367 T6376 prep In,had
R4717 T6368 T6367 pobj contrast,In
R4718 T6369 T6376 punct ",",had
R4719 T6370 T6376 nsubj cells,had
R4720 T6371 T6370 acl incubated,cells
R4721 T6372 T6371 prep with,incubated
R4722 T6373 T6372 pobj M-CSF,with
R4723 T6374 T6373 cc and,M-CSF
R4724 T6375 T6373 conj vehicle,M-CSF
R4725 T6376 T6376 ROOT had,had
R4726 T6377 T6378 advmod less,cleaved
R4727 T6378 T6379 amod cleaved,caspase-3
R4728 T6379 T6376 dobj caspase-3,had
R4729 T6380 T6379 prep than,caspase-3
R4730 T6381 T6380 pobj cells,than
R4731 T6382 T6381 acl incubated,cells
R4732 T6383 T6382 prep with,incubated
R4733 T6384 T6383 pobj vehicle,with
R4734 T6385 T6382 advmod alone,incubated
R4735 T6386 T6385 cc or,alone
R4736 T6387 T6385 conj M-CSF,alone
R4737 T6388 T6382 prep with,incubated
R4738 T6389 T6390 compound PKC,inhibitors
R4739 T6390 T6388 pobj inhibitors,with
R4740 T6391 T6376 punct .,had
R4741 T6392 T6393 det These,data
R4742 T6393 T6394 nsubj data,supported
R4743 T6394 T6394 ROOT supported,supported
R4744 T6395 T6396 det the,hypothesis
R4745 T6396 T6394 dobj hypothesis,supported
R4746 T6397 T6398 nsubj that,M-CSF-induced
R4747 T6398 T6400 amod M-CSF-induced,activity
R4748 T6399 T6400 amod NF-κB,activity
R4749 T6400 T6402 nsubjpass activity,regulated
R4750 T6401 T6402 auxpass was,regulated
R4751 T6402 T6394 conj regulated,supported
R4752 T6403 T6402 agent by,regulated
R4753 T6404 T6405 amod conventional,PKCs
R4754 T6405 T6403 pobj PKCs,by
R4755 T6406 T6405 punct ",",PKCs
R4756 T6407 T6408 neg not,novel
R4757 T6408 T6409 amod novel,PKCs
R4758 T6409 T6405 appos PKCs,PKCs
R4759 T6410 T6402 punct .,regulated
R4760 T6411 T6412 aux To,confirm
R4761 T6412 T6422 advcl confirm,treated
R4762 T6413 T6416 mark that,decreased
R4763 T6414 T6415 compound PKC,inhibition
R4764 T6415 T6416 nsubj inhibition,decreased
R4765 T6416 T6418 amod decreased,survival
R4766 T6417 T6418 compound cell,survival
R4767 T6418 T6412 dobj survival,confirm
R4768 T6419 T6422 punct ",",treated
R4769 T6420 T6422 nsubjpass cells,treated
R4770 T6421 T6422 auxpass were,treated
R4771 T6422 T6422 ROOT treated,treated
R4772 T6423 T6422 prep with,treated
R4773 T6424 T6423 pobj M-CSF,with
R4774 T6425 T6422 prep in,treated
R4775 T6426 T6427 det the,absence
R4776 T6427 T6425 pobj absence,in
R4777 T6428 T6427 cc or,absence
R4778 T6429 T6427 conj presence,absence
R4779 T6430 T6427 prep of,absence
R4780 T6431 T6432 compound PKC,inhibitors
R4781 T6432 T6430 pobj inhibitors,of
R4782 T6433 T6422 cc and,treated
R4783 T6434 T6436 advmod then,examined
R4784 T6435 T6436 advmod also,examined
R4785 T6436 T6422 conj examined,treated
R4786 T6437 T6436 prep for,examined
R4787 T6438 T6437 pobj apoptosis,for
R4788 T6439 T6436 agent by,examined
R4789 T6440 T6441 compound Annexin,V/PI
R4790 T6441 T6439 pobj V/PI,by
R4791 T6442 T6436 prep staining,examined
R4792 T6443 T6422 punct .,treated
R4793 T6444 T6445 compound PKC,inhibitors
R4794 T6445 T6451 nsubj inhibitors,reduced
R4795 T6446 T6445 prep in,inhibitors
R4796 T6447 T6448 det the,presence
R4797 T6448 T6446 pobj presence,in
R4798 T6449 T6448 prep of,presence
R4799 T6450 T6449 pobj M-CSF,of
R48 T80 T82 amod mononuclear,survival
R4800 T6451 T6451 ROOT reduced,reduced
R4801 T6452 T6453 det the,number
R4802 T6453 T6451 dobj number,reduced
R4803 T6454 T6453 prep of,number
R4804 T6455 T6456 compound Annexin,V/PI
R4805 T6456 T6458 nmod V/PI,MDMs
R4806 T6457 T6458 amod negative,MDMs
R4807 T6458 T6454 pobj MDMs,of
R4808 T6459 T6451 prep compared,reduced
R4809 T6460 T6459 prep to,compared
R4810 T6461 T6460 pobj cells,to
R4811 T6462 T6461 acl treated,cells
R4812 T6463 T6462 prep with,treated
R4813 T6464 T6463 pobj M-CSF,with
R4814 T6465 T6464 advmod alone,M-CSF
R4815 T6466 T6468 punct (,3B
R4816 T6467 T6468 compound Figure,3B
R4817 T6468 T6464 appos 3B,M-CSF
R4818 T6469 T6451 punct ),reduced
R4819 T6470 T6451 punct .,reduced
R4820 T6471 T6472 det These,observations
R4821 T6472 T6474 nsubj observations,suggested
R4822 T6473 T6474 advmod further,suggested
R4823 T6474 T6474 ROOT suggested,suggested
R4824 T6475 T6479 mark that,promoted
R4825 T6476 T6477 compound MDM,survival
R4826 T6477 T6479 nsubjpass survival,promoted
R4827 T6478 T6479 auxpass is,promoted
R4828 T6479 T6474 ccomp promoted,suggested
R4829 T6480 T6479 agent by,promoted
R4830 T6481 T6480 pobj M-CSF,by
R4831 T6482 T6479 cc and,promoted
R4832 T6483 T6484 advmod critically,involves
R4833 T6484 T6479 conj involves,promoted
R4834 T6485 T6486 amod conventional,PKCs
R4835 T6486 T6484 dobj PKCs,involves
R4836 T6487 T6486 cc and,PKCs
R4837 T6488 T6489 amod NF-κB,activity
R4838 T6489 T6486 conj activity,PKCs
R4839 T6490 T6474 punct .,suggested
R49 T81 T82 amod phagocyte,survival
R50 T82 T79 dobj survival,promotes
R51 T83 T82 cc and,survival
R5169 T7014 T7017 compound M-CSF-Induces,Activity
R5170 T7015 T7017 compound PKCα,Activity
R5171 T7016 T7017 compound Kinase,Activity
R5172 T7017 T7021 nsubj Activity,suggested
R5173 T7018 T7021 mark Since,suggested
R5174 T7019 T7020 poss our,data
R5175 T7020 T7021 nsubj data,suggested
R5176 T7021 T7041 advcl suggested,investigated
R5177 T7022 T7026 mark that,involved
R5178 T7023 T7024 amod conventional,PKCs
R5179 T7024 T7026 nsubjpass PKCs,involved
R5180 T7025 T7026 auxpass were,involved
R5181 T7026 T7021 ccomp involved,suggested
R5182 T7027 T7026 prep in,involved
R5183 T7028 T7027 pcomp activating,in
R5184 T7029 T7028 dobj NF-κB,activating
R5185 T7030 T7028 prep in,activating
R5186 T7031 T7030 pobj response,in
R5187 T7032 T7031 prep to,response
R5188 T7033 T7032 pobj M-CSF,to
R5189 T7034 T7033 prep in,M-CSF
R5190 T7035 T7037 amod primary,macrophages
R5191 T7036 T7037 amod human,macrophages
R5192 T7037 T7034 pobj macrophages,in
R5193 T7038 T7041 punct ",",investigated
R5194 T7039 T7041 nsubj we,investigated
R5195 T7040 T7041 advmod next,investigated
R5196 T7041 T7041 ROOT investigated,investigated
R5197 T7042 T7048 mark whether,was
R5198 T7043 T7045 det the,PKC
R5199 T7044 T7045 amod conventional,PKC
R52 T84 T82 conj proliferation,survival
R5200 T7045 T7048 nsubj PKC,was
R5201 T7046 T7048 punct ",",was
R5202 T7047 T7048 nsubj PKCα,was
R5203 T7048 T7041 ccomp was,investigated
R5204 T7049 T7051 det a,target
R5205 T7050 T7051 amod downstream,target
R5206 T7051 T7048 attr target,was
R5207 T7052 T7051 prep of,target
R5208 T7053 T7052 pobj M-CSF,of
R5209 T7054 T7051 prep in,target
R5210 T7055 T7056 amod human,MDMs
R5211 T7056 T7054 pobj MDMs,in
R5212 T7057 T7041 punct .,investigated
R5213 T7058 T7060 nsubjpass PKCα,immunoprecipitated
R5214 T7059 T7060 auxpass was,immunoprecipitated
R5215 T7060 T7060 ROOT immunoprecipitated,immunoprecipitated
R5216 T7061 T7060 prep from,immunoprecipitated
R5217 T7062 T7063 amod human,MDMs
R5218 T7063 T7061 pobj MDMs,from
R5219 T7064 T7060 prep at,immunoprecipitated
R5220 T7065 T7067 det the,time
R5221 T7066 T7067 amod indicated,time
R5222 T7067 T7064 pobj time,at
R5223 T7068 T7081 advmod points,performed
R5224 T7069 T7070 punct (,Figure
R5225 T7070 T7068 appos Figure,points
R5226 T7071 T7070 nummod 4,Figure
R5227 T7072 T7070 punct ),Figure
R5228 T7073 T7070 punct ",",Figure
R5229 T7074 T7070 cc and,Figure
R5230 T7075 T7081 advmod then,performed
R5231 T7076 T7079 det a,assay
R5232 T7077 T7079 compound PKC,assay
R5233 T7078 T7079 compound kinase,assay
R5234 T7079 T7081 nsubjpass assay,performed
R5235 T7080 T7081 auxpass was,performed
R5236 T7081 T7081 ROOT performed,performed
R5237 T7082 T7081 advcl using,performed
R5238 T7083 T7085 det a,peptide
R5239 T7084 T7085 amod fluorescein-tagged,peptide
R5240 T7085 T7082 dobj peptide,using
R5241 T7086 T7082 prep as,using
R5242 T7087 T7088 det a,substrate
R5243 T7088 T7086 pobj substrate,as
R5244 T7089 T7081 punct .,performed
R5245 T7090 T7091 mark As,shown
R5246 T7091 T7103 advcl shown,was
R5247 T7092 T7091 prep in,shown
R5248 T7093 T7092 pobj Figure,in
R5249 T7094 T7093 nummod 4,Figure
R5250 T7095 T7097 punct (,panel
R5251 T7096 T7097 amod upper,panel
R5252 T7097 T7093 appos panel,Figure
R5253 T7098 T7093 punct ),Figure
R5254 T7099 T7103 punct ",",was
R5255 T7100 T7102 det the,substrate
R5256 T7101 T7102 compound peptide,substrate
R5257 T7102 T7103 nsubj substrate,was
R5258 T7103 T7103 ROOT was,was
R5259 T7104 T7105 advmod maximally,phosphorylated
R5260 T7105 T7103 acomp phosphorylated,was
R5261 T7106 T7105 prep within,phosphorylated
R5262 T7107 T7108 nummod 10,minutes
R5263 T7108 T7106 pobj minutes,within
R5264 T7109 T7108 prep of,minutes
R5265 T7110 T7109 pobj stimulation,of
R5266 T7111 T7112 punct (,1.8-fold
R5267 T7112 T7105 appos 1.8-fold,phosphorylated
R5268 T7113 T7112 prep over,1.8-fold
R5269 T7114 T7115 amod resting,cells
R5270 T7115 T7113 pobj cells,over
R5271 T7116 T7112 punct ",",1.8-fold
R5272 T7117 T7118 amod lower,panel
R5273 T7118 T7112 appos panel,1.8-fold
R5274 T7119 T7112 punct ),1.8-fold
R5275 T7120 T7112 punct ",",1.8-fold
R5276 T7121 T7105 cc and,phosphorylated
R5277 T7122 T7123 advmod then,returned
R5278 T7123 T7103 conj returned,was
R5279 T7124 T7123 prep to,returned
R5280 T7125 T7126 amod basal,levels
R5281 T7126 T7124 pobj levels,to
R5282 T7127 T7123 prep by,returned
R5283 T7128 T7129 nummod 15,minutes
R5284 T7129 T7127 pobj minutes,by
R5285 T7130 T7123 punct .,returned
R5286 T7131 T7132 det This,effect
R5287 T7132 T7135 nsubjpass effect,seen
R5288 T7133 T7135 auxpass was,seen
R5289 T7134 T7135 neg not,seen
R5290 T7135 T7135 ROOT seen,seen
R5291 T7136 T7135 prep in,seen
R5292 T7137 T7138 amod M-CSF-stimulated,monocytes
R5293 T7138 T7136 pobj monocytes,in
R5294 T7139 T7146 advmod when,used
R5295 T7140 T7141 amod isogenic,antibodies
R5296 T7141 T7146 nsubjpass antibodies,used
R5297 T7142 T7143 punct (,IgG
R5298 T7143 T7141 appos IgG,antibodies
R5299 T7144 T7141 punct ),antibodies
R53 T85 T79 punct .,promotes
R5300 T7145 T7146 auxpass were,used
R5301 T7146 T7135 advcl used,seen
R5302 T7147 T7146 prep for,used
R5303 T7148 T7147 pobj immunoprecipitation,for
R5304 T7149 T7135 punct .,seen
R5305 T7150 T7151 amod Western,blots
R5306 T7151 T7160 nsubj blots,demonstrated
R5307 T7152 T7151 prep of,blots
R5308 T7153 T7154 amod identical,samples
R5309 T7154 T7152 pobj samples,of
R5310 T7155 T7151 acl using,blots
R5311 T7156 T7157 det an,antibody
R5312 T7157 T7155 dobj antibody,using
R5313 T7158 T7155 advcl recognizing,using
R5314 T7159 T7160 nsubj PKCα,demonstrated
R5315 T7160 T7160 ROOT demonstrated,demonstrated
R5316 T7161 T7167 mark that,assayed
R5317 T7162 T7163 amod equal,amounts
R5318 T7163 T7167 nsubjpass amounts,assayed
R5319 T7164 T7163 prep of,amounts
R5320 T7165 T7164 pobj PKCα,of
R5321 T7166 T7167 auxpass were,assayed
R5322 T7167 T7160 ccomp assayed,demonstrated
R5323 T7168 T7169 punct (,Figure
R5324 T7169 T7167 oprd Figure,assayed
R5325 T7170 T7169 nummod 4,Figure
R5326 T7171 T7169 punct ",",Figure
R5327 T7172 T7173 amod middle,panel
R5328 T7173 T7169 appos panel,Figure
R5329 T7174 T7169 punct ),Figure
R5330 T7175 T7160 punct .,demonstrated
R54 T86 T88 det The,factor
R55 T87 T88 compound transcription,factor
R5538 T7492 T7493 amod M-CSF-Dependent,Activation
R5539 T7493 T7498 nsubj Activation,Regulate
R5540 T7494 T7493 prep of,Activation
R5541 T7495 T7494 pobj PKC,of
R5542 T7496 T7498 aux Does,Regulate
R5543 T7497 T7498 neg Not,Regulate
R5544 T7498 T7498 ROOT Regulate,Regulate
R5545 T7499 T7506 det the,activation
R5546 T7500 T7501 amod Classical,NF-κB
R5547 T7501 T7506 nmod NF-κB,activation
R5548 T7502 T7506 nummod p65,activation
R5549 T7503 T7504 compound Activation,Pathway
R5550 T7504 T7506 nmod Pathway,activation
R5551 T7505 T7506 amod Canonical,activation
R5552 T7506 T7509 nsubj activation,occurs
R5553 T7507 T7506 prep of,activation
R5554 T7508 T7507 pobj NF-κB,of
R5555 T7509 T7498 ccomp occurs,Regulate
R5556 T7510 T7509 prep via,occurs
R5557 T7511 T7510 pobj phosphorylation,via
R5558 T7512 T7511 cc and,phosphorylation
R5559 T7513 T7511 conj degradation,phosphorylation
R5560 T7514 T7513 prep of,degradation
R5561 T7515 T7516 nsubj IκBα,leading
R5562 T7516 T7498 advcl leading,Regulate
R5563 T7517 T7516 prep to,leading
R5564 T7518 T7519 det the,release
R5565 T7519 T7517 pobj release,to
R5566 T7520 T7519 cc and,release
R5567 T7521 T7522 amod nuclear,translocation
R5568 T7522 T7519 conj translocation,release
R5569 T7523 T7522 prep of,translocation
R5570 T7524 T7527 det the,heterodimer
R5571 T7525 T7527 nmod NF-κB,heterodimer
R5572 T7526 T7527 nummod p50/p65,heterodimer
R5573 T7527 T7523 pobj heterodimer,of
R5574 T7528 T7529 aux to,transactivate
R5575 T7529 T7516 xcomp transactivate,leading
R5576 T7530 T7531 compound target,genes
R5577 T7531 T7529 dobj genes,transactivate
R5578 T7532 T7534 nmod [,]
R5579 T7533 T7534 nummod 37,]
R5580 T7534 T7529 conj ],transactivate
R5581 T7535 T7534 punct ",",]
R5582 T7536 T7538 nmod [,]
R5583 T7537 T7538 nummod 39,]
R5584 T7538 T7534 appos ],]
R5585 T7539 T7498 punct .,Regulate
R5586 T7540 T7544 mark Since,was
R5587 T7541 T7543 amod conventional,activity
R5588 T7542 T7543 compound PKC,activity
R5589 T7543 T7544 nsubj activity,was
R5590 T7544 T7554 advcl was,investigated
R5591 T7545 T7544 acomp important,was
R5592 T7546 T7545 prep in,important
R5593 T7547 T7546 pcomp regulating,in
R5594 T7548 T7550 amod M-CSF-induced,activation
R5595 T7549 T7550 compound NF-κB,activation
R5596 T7550 T7547 dobj activation,regulating
R5597 T7551 T7554 punct ",",investigated
R5598 T7552 T7554 nsubj we,investigated
R5599 T7553 T7554 advmod next,investigated
R56 T88 T94 nsubj factor,is
R5600 T7554 T7554 ROOT investigated,investigated
R5601 T7555 T7559 mark whether,regulated
R5602 T7556 T7557 compound IκBα,degradation
R5603 T7557 T7559 nsubjpass degradation,regulated
R5604 T7558 T7559 auxpass was,regulated
R5605 T7559 T7554 ccomp regulated,investigated
R5606 T7560 T7559 agent by,regulated
R5607 T7561 T7560 pobj PKC,by
R5608 T7562 T7554 punct .,investigated
R5609 T7563 T7565 nsubjpass Cells,treated
R5610 T7564 T7565 auxpass were,treated
R5611 T7565 T7565 ROOT treated,treated
R5612 T7566 T7565 prep with,treated
R5613 T7567 T7566 pobj cyclohexamide,with
R5614 T7568 T7567 punct (,cyclohexamide
R5615 T7569 T7567 appos CHX,cyclohexamide
R5616 T7570 T7567 punct ),cyclohexamide
R5617 T7571 T7572 aux to,inhibit
R5618 T7572 T7565 advcl inhibit,treated
R5619 T7573 T7574 compound protein,synthesis
R5620 T7574 T7572 dobj synthesis,inhibit
R5621 T7575 T7574 prep of,synthesis
R5622 T7576 T7575 pobj IκBα,of
R5623 T7577 T7572 punct ",",inhibit
R5624 T7578 T7572 cc and,inhibit
R5625 T7579 T7580 poss its,degradation
R5626 T7580 T7582 nsubjpass degradation,followed
R5627 T7581 T7582 auxpass was,followed
R5628 T7582 T7565 conj followed,treated
R5629 T7583 T7582 punct .,followed
R5630 T7584 T7585 mark As,shown
R5631 T7585 T7596 advcl shown,alter
R5632 T7586 T7585 prep in,shown
R5633 T7587 T7586 pobj Figure,in
R5634 T7588 T7587 nummod 5A,Figure
R5635 T7589 T7585 punct ",",shown
R5636 T7590 T7591 compound PKC,inhibition
R5637 T7591 T7596 nsubj inhibition,alter
R5638 T7592 T7591 prep with,inhibition
R5639 T7593 T7592 pobj Ro-31-8220,with
R5640 T7594 T7596 aux did,alter
R5641 T7595 T7596 neg not,alter
R5642 T7596 T7596 ROOT alter,alter
R5643 T7597 T7599 amod M-CSF-induced,degradation
R5644 T7598 T7599 compound IκBα,degradation
R5645 T7599 T7596 dobj degradation,alter
R5646 T7600 T7596 punct ",",alter
R5647 T7601 T7596 advcl suggesting,alter
R5648 T7602 T7606 mark that,augmented
R5649 T7603 T7605 amod M-CSF-induced,activity
R5650 T7604 T7605 compound PKC,activity
R5651 T7605 T7606 nsubj activity,augmented
R5652 T7606 T7601 ccomp augmented,suggesting
R5653 T7607 T7609 amod NF-κB,activity
R5654 T7608 T7609 amod transcriptional,activity
R5655 T7609 T7606 dobj activity,augmented
R5656 T7610 T7609 prep by,activity
R5657 T7611 T7613 det an,pathway
R5658 T7612 T7613 amod alternative,pathway
R5659 T7613 T7610 pobj pathway,by
R5660 T7614 T7613 punct ",",pathway
R5661 T7615 T7606 prep like,augmented
R5662 T7616 T7617 amod post-translational,modification
R5663 T7617 T7615 pobj modification,like
R5664 T7618 T7617 prep of,modification
R5665 T7619 T7620 compound NF-κB,p65
R5666 T7620 T7618 pobj p65,of
R5667 T7621 T7596 punct .,alter
R57 T89 T90 compound Nuclear,Factor-kappaB
R58 T90 T88 appos Factor-kappaB,factor
R59 T91 T92 punct (,NF-κB
R5979 T8089 T8090 nsubj M-CSF,Induces
R5980 T8090 T8091 compound Induces,Phosphorylation
R5981 T8091 T8112 nsubj Phosphorylation,sought
R5982 T8092 T8091 prep of,Phosphorylation
R5983 T8093 T8094 compound NF-κB,Ser276
R5984 T8094 T8092 pobj Ser276,of
R5985 T8095 T8091 prep in,Phosphorylation
R5986 T8096 T8098 det a,Fashion
R5987 T8097 T8098 amod PKC-dependent,Fashion
R5988 T8098 T8095 pobj Fashion,in
R5989 T8099 T8103 mark Since,regulate
R5990 T8100 T8103 nsubj M-CSF,regulate
R5991 T8101 T8103 aux did,regulate
R5992 T8102 T8103 neg not,regulate
R5993 T8103 T8112 advcl regulate,sought
R5994 T8104 T8105 amod NF-κB,activation
R5995 T8105 T8103 dobj activation,regulate
R5996 T8106 T8103 prep by,regulate
R5997 T8107 T8106 pcomp influencing,by
R5998 T8108 T8107 dobj IκBα,influencing
R5999 T8109 T8112 punct ",",sought
R60 T92 T90 appos NF-κB,Factor-kappaB
R6000 T8110 T8112 nsubj we,sought
R6001 T8111 T8112 advmod next,sought
R6002 T8112 T8112 ROOT sought,sought
R6003 T8113 T8114 aux to,determine
R6004 T8114 T8112 xcomp determine,sought
R6005 T8115 T8117 mark if,affected
R6006 T8116 T8117 nsubj M-CSF,affected
R6007 T8117 T8114 ccomp affected,determine
R6008 T8118 T8119 compound NF-κB,p65
R6009 T8119 T8117 dobj p65,affected
R6010 T8120 T8117 prep by,affected
R6011 T8121 T8122 amod post-translational,mechanisms
R6012 T8122 T8120 pobj mechanisms,by
R6013 T8123 T8112 punct .,sought
R6014 T8124 T8127 advmod Thus,examined
R6015 T8125 T8127 punct ",",examined
R6016 T8126 T8127 nsubj we,examined
R6017 T8127 T8127 ROOT examined,examined
R6018 T8128 T8129 det the,phosphorylation
R6019 T8129 T8127 dobj phosphorylation,examined
R6020 T8130 T8129 prep of,phosphorylation
R6021 T8131 T8130 pobj NF-κB,of
R6022 T8132 T8131 nummod p65,NF-κB
R6023 T8133 T8129 prep with,phosphorylation
R6024 T8134 T8136 amod specific,p65
R6025 T8135 T8136 compound phospho-NFκB,p65
R6026 T8136 T8133 pobj p65,with
R6027 T8137 T8136 punct (,p65
R6028 T8138 T8136 appos Ser276,p65
R6029 T8139 T8138 cc and,Ser276
R6030 T8140 T8138 conj Ser536,Ser276
R6031 T8141 T8136 punct ),p65
R6032 T8142 T8136 appos antibodies,p65
R6033 T8143 T8127 punct .,examined
R6034 T8144 T8145 nsubj M-CSF,induced
R6035 T8145 T8145 ROOT induced,induced
R6036 T8146 T8147 det the,phosphorylation
R6037 T8147 T8145 dobj phosphorylation,induced
R6038 T8148 T8147 prep of,phosphorylation
R6039 T8149 T8148 pobj Ser276,of
R6040 T8150 T8147 cc but,phosphorylation
R6041 T8151 T8152 neg not,Ser536
R6042 T8152 T8147 conj Ser536,phosphorylation
R6043 T8153 T8152 prep of,Ser536
R6044 T8154 T8153 pobj NF-κB,of
R6045 T8155 T8154 nummod p65,NF-κB
R6046 T8156 T8152 prep in,Ser536
R6047 T8157 T8156 pobj MDMs,in
R6048 T8158 T8145 punct .,induced
R6049 T8159 T8168 prep Compared,reduced
R6050 T8160 T8159 prep to,Compared
R6051 T8161 T8160 pobj vehicle,to
R6052 T8162 T8168 punct ",",reduced
R6053 T8163 T8166 det the,inhibitor
R6054 T8164 T8166 amod general,inhibitor
R6055 T8165 T8166 compound PKC,inhibitor
R6056 T8166 T8168 nsubj inhibitor,reduced
R6057 T8167 T8168 nsubj Ro-31-8220,reduced
R6058 T8168 T8168 ROOT reduced,reduced
R6059 T8169 T8170 compound Ser276,phosphorylation
R6060 T8170 T8168 dobj phosphorylation,reduced
R6061 T8171 T8168 punct ",",reduced
R6062 T8172 T8168 cc but,reduced
R6063 T8173 T8174 neg not,Ser536
R6064 T8174 T8168 conj Ser536,reduced
R6065 T8175 T8174 punct ",",Ser536
R6066 T8176 T8174 appos phosphorylation,Ser536
R6067 T8177 T8176 prep in,phosphorylation
R6068 T8178 T8179 amod M-CSF-stimulated,cells
R6069 T8179 T8177 pobj cells,in
R6070 T8180 T8182 punct (,5B
R6071 T8181 T8182 compound Figure,5B
R6072 T8182 T8176 appos 5B,phosphorylation
R6073 T8183 T8182 punct ),5B
R6074 T8184 T8168 punct .,reduced
R6075 T8185 T8198 advmod Furthermore,was
R6076 T8186 T8198 punct ",",was
R6077 T8187 T8190 amod M-CSF-stimulated,phosphorylation
R6078 T8188 T8190 nmod NF-κB,phosphorylation
R6079 T8189 T8190 nummod p65,phosphorylation
R6080 T8190 T8198 nsubj phosphorylation,was
R6081 T8191 T8190 prep at,phosphorylation
R6082 T8192 T8191 pobj residue,at
R6083 T8193 T8190 conj Ser276,phosphorylation
R6084 T8194 T8193 prep in,Ser276
R6085 T8195 T8194 pobj RAW,in
R6086 T8196 T8197 nummod 264.7,cells
R6087 T8197 T8190 conj cells,phosphorylation
R6088 T8198 T8198 ROOT was,was
R6089 T8199 T8198 advmod also,was
R6090 T8200 T8201 compound PKC,dependent
R6091 T8201 T8198 attr dependent,was
R6092 T8202 T8204 punct (,5C
R6093 T8203 T8204 compound Figure,5C
R6094 T8204 T8201 appos 5C,dependent
R6095 T8205 T8204 punct ),5C
R6096 T8206 T8198 punct .,was
R6097 T8207 T8208 det These,studies
R6098 T8208 T8209 nsubj studies,suggested
R6099 T8209 T8209 ROOT suggested,suggested
R61 T93 T90 punct ),Factor-kappaB
R6100 T8210 T8215 mark that,regulated
R6101 T8211 T8215 nsubj PKC,regulated
R6102 T8212 T8211 punct (,PKC
R6103 T8213 T8211 appos s,PKC
R6104 T8214 T8211 punct ),PKC
R6105 T8215 T8209 ccomp regulated,suggested
R6106 T8216 T8217 compound Ser276,phosphorylation
R6107 T8217 T8215 dobj phosphorylation,regulated
R6108 T8218 T8217 cc but,phosphorylation
R6109 T8219 T8220 neg not,Ser536
R6110 T8220 T8217 conj Ser536,phosphorylation
R6111 T8221 T8220 prep in,Ser536
R6112 T8222 T8224 preconj both,MDMs
R6113 T8223 T8224 amod human,MDMs
R6114 T8224 T8221 pobj MDMs,in
R6115 T8225 T8224 cc and,MDMs
R6116 T8226 T8227 compound mouse,macrophages
R6117 T8227 T8224 conj macrophages,MDMs
R6118 T8228 T8220 prep after,Ser536
R6119 T8229 T8230 compound M-CSF,stimulation
R6120 T8230 T8228 pobj stimulation,after
R6121 T8231 T8209 punct .,suggested
R6122 T8232 T8234 nsubj We,performed
R6123 T8233 T8234 advmod next,performed
R6124 T8234 T8234 ROOT performed,performed
R6125 T8235 T8236 amod cellular,fractionation
R6126 T8236 T8234 dobj fractionation,performed
R6127 T8237 T8238 aux to,identify
R6128 T8238 T8234 advcl identify,performed
R6129 T8239 T8241 det the,location
R6130 T8240 T8241 amod cellular,location
R6131 T8241 T8238 dobj location,identify
R6132 T8242 T8241 prep of,location
R6133 T8243 T8245 amod phosphorylated,p65
R6134 T8244 T8245 compound NF-κB,p65
R6135 T8245 T8242 pobj p65,of
R6136 T8246 T8245 prep in,p65
R6137 T8247 T8249 amod Raw,cells
R6138 T8248 T8249 nummod 264.7,cells
R6139 T8249 T8246 pobj cells,in
R6140 T8250 T8234 punct .,performed
R6141 T8251 T8253 amod Non-phosphorylated,p65
R6142 T8252 T8253 compound NF-κB,p65
R6143 T8253 T8255 nsubjpass p65,located
R6144 T8254 T8255 auxpass was,located
R6145 T8255 T8255 ROOT located,located
R6146 T8256 T8255 prep in,located
R6147 T8257 T8258 preconj both,cytosolic
R6148 T8258 T8261 amod cytosolic,fractions
R6149 T8259 T8258 cc and,cytosolic
R6150 T8260 T8258 conj nuclear,cytosolic
R6151 T8261 T8256 pobj fractions,in
R6152 T8262 T8255 punct ",",located
R6153 T8263 T8255 cc but,located
R6154 T8264 T8272 dep phosphorylated,located
R6155 T8265 T8272 nsubjpass Ser276,located
R6156 T8266 T8265 cc and,Ser276
R6157 T8267 T8268 compound Ser536,NF-κB
R6158 T8268 T8265 conj NF-κB,Ser276
R6159 T8269 T8268 nummod p65,NF-κB
R6160 T8270 T8272 auxpass was,located
R6161 T8271 T8272 advmod primarily,located
R6162 T8272 T8255 conj located,located
R6163 T8273 T8272 prep in,located
R6164 T8274 T8275 amod nuclear,fraction
R6165 T8275 T8273 pobj fraction,in
R6166 T8276 T8272 prep after,located
R6167 T8277 T8278 compound M-CSF,stimulation
R6168 T8278 T8276 pobj stimulation,after
R6169 T8279 T8280 punct (,Figure
R6170 T8280 T8278 appos Figure,stimulation
R6171 T8281 T8280 nummod 5D,Figure
R6172 T8282 T8272 punct ),located
R6173 T8283 T8255 punct .,located
R6174 T8284 T8293 advmod Notably,found
R6175 T8285 T8293 punct ",",found
R6176 T8286 T8287 amod constitutive,phosphorylation
R6177 T8287 T8293 nsubjpass phosphorylation,found
R6178 T8288 T8287 prep of,phosphorylation
R6179 T8289 T8290 compound Ser536,NF-κB
R6180 T8290 T8288 pobj NF-κB,of
R6181 T8291 T8290 nummod p65,NF-κB
R6182 T8292 T8293 auxpass was,found
R6183 T8293 T8293 ROOT found,found
R6184 T8294 T8293 prep in,found
R6185 T8295 T8296 det these,cells
R6186 T8296 T8294 pobj cells,in
R6187 T8297 T8293 punct .,found
R6188 T8298 T8301 advmod Importantly,reduced
R6189 T8299 T8301 punct ",",reduced
R6190 T8300 T8301 nsubj Ro-31-8220,reduced
R6191 T8301 T8301 ROOT reduced,reduced
R6192 T8302 T8304 amod M-CSF-induced,phosphorylation
R6193 T8303 T8304 compound Ser276,phosphorylation
R6194 T8304 T8301 dobj phosphorylation,reduced
R6195 T8305 T8304 prep of,phosphorylation
R6196 T8306 T8305 pobj NF-κB,of
R6197 T8307 T8306 nummod p65,NF-κB
R6198 T8308 T8301 prep in,reduced
R6199 T8309 T8314 preconj both,fractions
R62 T94 T94 ROOT is,is
R6200 T8310 T8314 det the,fractions
R6201 T8311 T8314 amod cytosolic,fractions
R6202 T8312 T8311 cc and,cytosolic
R6203 T8313 T8311 conj nuclear,cytosolic
R6204 T8314 T8308 pobj fractions,in
R6205 T8315 T8301 punct ",",reduced
R6206 T8316 T8322 mark while,was
R6207 T8317 T8318 compound M-CSF-induced,NF-κB
R6208 T8318 T8322 nsubj NF-κB,was
R6209 T8319 T8321 nummod p65,phosphorylation
R6210 T8320 T8321 compound Ser536,phosphorylation
R6211 T8321 T8322 nsubj phosphorylation,was
R6212 T8322 T8301 advcl was,reduced
R6213 T8323 T8322 acomp present,was
R6214 T8324 T8323 prep in,present
R6215 T8325 T8326 det the,nucleus
R6216 T8326 T8324 pobj nucleus,in
R6217 T8327 T8322 advmod regardless,was
R6218 T8328 T8327 prep of,regardless
R6219 T8329 T8330 compound PKC,inhibition
R6220 T8330 T8328 pobj inhibition,of
R6221 T8331 T8301 punct .,reduced
R6222 T8332 T8333 det These,observations
R6223 T8333 T8334 nsubj observations,indicate
R6224 T8334 T8334 ROOT indicate,indicate
R6225 T8335 T8341 mark that,regulated
R6226 T8336 T8337 compound M-CSF-induced,Ser276
R6227 T8337 T8341 nsubjpass Ser276,regulated
R6228 T8338 T8337 cc and,Ser276
R6229 T8339 T8337 conj Ser536,Ser276
R6230 T8340 T8341 auxpass are,regulated
R6231 T8341 T8334 ccomp regulated,indicate
R6232 T8342 T8341 advmod differently,regulated
R6233 T8343 T8341 agent by,regulated
R6234 T8344 T8346 amod conventional,activation
R6235 T8345 T8346 compound PKC,activation
R6236 T8346 T8343 pobj activation,by
R6237 T8347 T8346 prep in,activation
R6238 T8348 T8349 compound mononuclear,phagocytes
R6239 T8349 T8347 pobj phagocytes,in
R6240 T8350 T8334 punct .,indicate
R6241 T8351 T8352 det The,purity
R6242 T8352 T8361 nsubjpass purity,confirmed
R6243 T8353 T8352 prep of,purity
R6244 T8354 T8355 det the,cytosol
R6245 T8355 T8353 pobj cytosol,of
R6246 T8356 T8355 cc and,cytosol
R6247 T8357 T8358 amod nuclear,cell
R6248 T8358 T8359 compound cell,fractions
R6249 T8359 T8352 conj fractions,purity
R6250 T8360 T8361 auxpass was,confirmed
R6251 T8361 T8361 ROOT confirmed,confirmed
R6252 T8362 T8361 agent by,confirmed
R6253 T8363 T8362 pcomp immunoblotting,by
R6254 T8364 T8363 prep with,immunoblotting
R6255 T8365 T8364 pobj GAPDH,with
R6256 T8366 T8365 cc and,GAPDH
R6257 T8367 T8368 compound Lamin,B
R6258 T8368 T8365 conj B,GAPDH
R6259 T8369 T8363 punct ",",immunoblotting
R6260 T8370 T8372 intj respectively,Figure
R6261 T8371 T8372 punct (,Figure
R6262 T8372 T8361 npadvmod Figure,confirmed
R6263 T8373 T8372 nummod 5D,Figure
R6264 T8374 T8372 punct ),Figure
R6265 T8375 T8361 punct .,confirmed
R63 T95 T97 det a,regulator
R64 T96 T97 amod key,regulator
R646 T1018 T1020 nsubjpass Monocytes,produced
R647 T1019 T1020 auxpass are,produced
R648 T1020 T1020 ROOT produced,produced
R649 T1021 T1020 prep in,produced
R65 T97 T94 attr regulator,is
R650 T1022 T1024 det the,marrow
R651 T1023 T1024 compound bone,marrow
R652 T1024 T1021 pobj marrow,in
R653 T1025 T1020 cc and,produced
R654 T1026 T1020 conj circulate,produced
R655 T1027 T1026 prep in,circulate
R656 T1028 T1027 pobj blood,in
R657 T1029 T1026 prep for,circulate
R658 T1030 T1029 pobj 24,for
R659 T1031 T1032 nummod 48,hours
R66 T98 T97 prep of,regulator
R660 T1032 T1030 appos hours,24
R661 T1033 T1035 nmod [,]
R662 T1034 T1035 nummod 1,]
R663 T1035 T1032 appos ],hours
R664 T1036 T1020 punct .,produced
R665 T1037 T1044 prep In,die
R666 T1038 T1039 det the,absence
R667 T1039 T1037 pobj absence,In
R668 T1040 T1039 prep of,absence
R669 T1041 T1040 pobj serum,of
R67 T99 T98 pobj genes,of
R670 T1042 T1044 punct ",",die
R671 T1043 T1044 nsubj monocytes,die
R672 T1044 T1044 ROOT die,die
R673 T1045 T1044 prep via,die
R674 T1046 T1047 compound apoptosis,[
R675 T1047 T1045 pobj [,via
R676 T1048 T1049 nummod 1,]
R677 T1049 T1044 advcl ],die
R678 T1050 T1049 punct ",",]
R679 T1051 T1053 nmod [,]
R68 T100 T99 acl involved,genes
R680 T1052 T1053 nummod 2,]
R681 T1053 T1049 appos ],]
R682 T1054 T1044 punct .,die
R683 T1055 T1057 nmod Macrophage,factor
R684 T1056 T1057 amod colony-stimulating,factor
R685 T1057 T1061 nsubj factor,stimulates
R686 T1058 T1059 punct (,M-CSF
R687 T1059 T1057 appos M-CSF,factor
R688 T1060 T1057 punct ),factor
R689 T1061 T1061 ROOT stimulates,stimulates
R69 T101 T100 prep in,involved
R690 T1062 T1064 amod mononuclear,survival
R691 T1063 T1064 amod phagocyte,survival
R692 T1064 T1069 nmod survival,]
R693 T1065 T1064 cc and,survival
R694 T1066 T1067 compound differentiation,[
R695 T1067 T1064 conj [,survival
R696 T1068 T1069 nummod 3,]
R697 T1069 T1061 dobj ],stimulates
R698 T1070 T1061 punct .,stimulates
R699 T1071 T1079 advmod Importantly,associated
R70 T102 T105 amod M-CSF-induced,survival
R700 T1072 T1079 punct ",",associated
R701 T1073 T1075 amod M-CSF-mediated,survival
R702 T1074 T1075 compound cell,survival
R703 T1075 T1079 nsubjpass survival,associated
R704 T1076 T1075 cc and,survival
R705 T1077 T1075 conj activation,survival
R706 T1078 T1079 auxpass is,associated
R707 T1079 T1079 ROOT associated,associated
R7074 T9427 T9428 compound M-CSF-dependent,PKC
R7075 T9428 T9429 nsubj PKC,Regulates
R7076 T9429 T9429 ROOT Regulates,Regulates
R7077 T9430 T9431 compound NF-κB-targeted,Genes
R7078 T9431 T9432 compound Genes,NF-κB
R7079 T9432 T9433 nsubj NF-κB,induces
R708 T1080 T1079 prep with,associated
R7080 T9433 T9429 ccomp induces,Regulates
R7081 T9434 T9435 det a,number
R7082 T9435 T9433 dobj number,induces
R7083 T9436 T9435 prep of,number
R7084 T9437 T9438 amod downstream,genes
R7085 T9438 T9436 pobj genes,of
R7086 T9439 T9438 punct ",",genes
R7087 T9440 T9438 prep including,genes
R7088 T9441 T9443 det the,family
R7089 T9442 T9443 compound IκB,family
R709 T1081 T1082 det a,variety
R7090 T9443 T9440 pobj family,including
R7091 T9444 T9429 punct .,Regulates
R7092 T9445 T9453 prep Among,induced
R7093 T9446 T9448 det the,molecules
R7094 T9447 T9448 compound IκB,molecules
R7095 T9448 T9445 pobj molecules,Among
R7096 T9449 T9453 punct ",",induced
R7097 T9450 T9453 nsubjpass IκBα,induced
R7098 T9451 T9453 auxpass is,induced
R7099 T9452 T9453 advmod highly,induced
R71 T103 T105 compound mononuclear,survival
R710 T1082 T1080 pobj variety,with
R7100 T9453 T9453 ROOT induced,induced
R7101 T9454 T9453 agent by,induced
R7102 T9455 T9456 amod NF-κB,activation
R7103 T9456 T9459 nmod activation,]
R7104 T9457 T9459 nmod [,]
R7105 T9458 T9459 nummod 40,]
R7106 T9459 T9454 pobj ],by
R7107 T9460 T9453 punct .,induced
R7108 T9461 T9462 aux Having,shown
R7109 T9462 T9474 advcl shown,determined
R711 T1083 T1082 prep of,variety
R7110 T9463 T9465 mark that,regulated
R7111 T9464 T9465 nsubj PKC,regulated
R7112 T9465 T9462 ccomp regulated,shown
R7113 T9466 T9467 amod NF-κB,activity
R7114 T9467 T9465 dobj activity,regulated
R7115 T9468 T9465 prep in,regulated
R7116 T9469 T9470 amod M-CSF-stimulated,MDMs
R7117 T9470 T9468 pobj MDMs,in
R7118 T9471 T9474 punct ",",determined
R7119 T9472 T9474 nsubj we,determined
R712 T1084 T1085 amod human,diseases
R7120 T9473 T9474 advmod next,determined
R7121 T9474 T9474 ROOT determined,determined
R7122 T9475 T9480 mark whether,decreased
R7123 T9476 T9480 nsubj inhibition,decreased
R7124 T9477 T9476 prep of,inhibition
R7125 T9478 T9479 compound PKC,activity
R7126 T9479 T9477 pobj activity,of
R7127 T9480 T9474 ccomp decreased,determined
R7128 T9481 T9480 dobj expression,decreased
R7129 T9482 T9481 prep of,expression
R713 T1085 T1083 pobj diseases,of
R7130 T9483 T9484 amod NF-κB-regulated,genes
R7131 T9484 T9482 pobj genes,of
R7132 T9485 T9474 punct .,determined
R7133 T9486 T9487 nsubj We,treated
R7134 T9487 T9487 ROOT treated,treated
R7135 T9488 T9489 det both,MDMs
R7136 T9489 T9487 dobj MDMs,treated
R7137 T9490 T9489 cc and,MDMs
R7138 T9491 T9489 conj RAW,MDMs
R7139 T9492 T9493 nummod 264.7,cells
R714 T1086 T1085 punct ",",diseases
R7140 T9493 T9489 conj cells,MDMs
R7141 T9494 T9493 prep with,cells
R7142 T9495 T9498 det the,Ro-31-8220
R7143 T9496 T9498 compound PKC,Ro-31-8220
R7144 T9497 T9498 compound inhibitor,Ro-31-8220
R7145 T9498 T9494 pobj Ro-31-8220,with
R7146 T9499 T9487 prep for,treated
R7147 T9500 T9501 nummod 30,minutes
R7148 T9501 T9499 pobj minutes,for
R7149 T9502 T9487 cc and,treated
R715 T1087 T1085 prep including,diseases
R7150 T9503 T9504 advmod then,stimulated
R7151 T9504 T9487 conj stimulated,treated
R7152 T9505 T9504 prep with,stimulated
R7153 T9506 T9505 pobj M-CSF,with
R7154 T9507 T9487 punct .,treated
R7155 T9508 T9509 compound IκBα,gene
R7156 T9509 T9511 nsubjpass gene,measured
R7157 T9510 T9511 auxpass was,measured
R7158 T9511 T9511 ROOT measured,measured
R7159 T9512 T9511 agent by,measured
R716 T1088 T1087 pobj atherosclerosis,including
R7160 T9513 T9512 pobj qRT-PCR,by
R7161 T9514 T9511 punct .,measured
R7162 T9515 T9516 mark As,shown
R7163 T9516 T9524 advcl shown,enhanced
R7164 T9517 T9516 prep in,shown
R7165 T9518 T9519 compound Figures,6A
R7166 T9519 T9517 pobj 6A,in
R7167 T9520 T9519 cc and,6A
R7168 T9521 T9519 conj 6B,6A
R7169 T9522 T9521 punct ",",6B
R717 T1089 T1088 punct ",",atherosclerosis
R7170 T9523 T9524 nsubj M-CSF,enhanced
R7171 T9524 T9524 ROOT enhanced,enhanced
R7172 T9525 T9527 compound IκBα,expression
R7173 T9526 T9527 compound gene,expression
R7174 T9527 T9524 dobj expression,enhanced
R7175 T9528 T9527 cc and,expression
R7176 T9529 T9530 compound PKC,inhibition
R7177 T9530 T9527 conj inhibition,expression
R7178 T9531 T9527 prep by,expression
R7179 T9532 T9531 pobj Ro-31-8220,by
R718 T1090 T1092 nmod transplant,sclerosis
R7180 T9533 T9536 amod decreased,expression
R7181 T9534 T9536 compound IκBα,expression
R7182 T9535 T9536 compound gene,expression
R7183 T9536 T9527 conj expression,expression
R7184 T9537 T9524 prep in,enhanced
R7185 T9538 T9539 preconj both,MDMs
R7186 T9539 T9537 pobj MDMs,in
R7187 T9540 T9539 cc and,MDMs
R7188 T9541 T9539 conj RAW,MDMs
R7189 T9542 T9543 nummod 264.7,cells
R719 T1091 T1092 amod vascular,sclerosis
R7190 T9543 T9539 conj cells,MDMs
R7191 T9544 T9543 punct (,cells
R7192 T9545 T9543 conj p,cells
R7193 T9546 T9547 nmod <,0.01
R7194 T9547 T9545 npadvmod 0.01,p
R7195 T9548 T9545 punct ),p
R7196 T9549 T9524 punct ",",enhanced
R7197 T9550 T9524 advcl demonstrating,enhanced
R7198 T9551 T9553 mark that,affected
R7199 T9552 T9553 nsubj PKC,affected
R72 T104 T105 compound phagocyte,survival
R720 T1092 T1088 conj sclerosis,atherosclerosis
R7200 T9553 T9550 ccomp affected,demonstrating
R7201 T9554 T9556 amod NF-κB-regulated,expression
R7202 T9555 T9556 compound gene,expression
R7203 T9556 T9553 dobj expression,affected
R7204 T9557 T9553 prep in,affected
R7205 T9558 T9557 pobj macrophages,in
R7206 T9559 T9524 punct .,enhanced
R7207 T9560 T9562 aux To,define
R7208 T9561 T9562 advmod further,define
R7209 T9562 T9578 advcl define,examined
R721 T1093 T1092 cc and,sclerosis
R7210 T9563 T9564 det the,role
R7211 T9564 T9562 dobj role,define
R7212 T9565 T9564 prep of,role
R7213 T9566 T9565 pobj PKC,of
R7214 T9567 T9564 prep in,role
R7215 T9568 T9567 pcomp mediating,in
R7216 T9569 T9571 amod human,survival
R7217 T9570 T9571 compound MDM,survival
R7218 T9571 T9568 dobj survival,mediating
R7219 T9572 T9568 prep in,mediating
R722 T1094 T1095 compound breast,cancer
R7220 T9573 T9572 pobj response,in
R7221 T9574 T9573 prep to,response
R7222 T9575 T9574 pobj M-CSF,to
R7223 T9576 T9578 punct ",",examined
R7224 T9577 T9578 nsubj we,examined
R7225 T9578 T9578 ROOT examined,examined
R7226 T9579 T9580 det the,expression
R7227 T9580 T9578 dobj expression,examined
R7228 T9581 T9580 prep of,expression
R7229 T9582 T9584 det the,gene
R723 T1095 T1096 compound cancer,metastasis
R7230 T9583 T9584 compound anti-apoptotic,gene
R7231 T9584 T9581 pobj gene,of
R7232 T9585 T9584 appos BCL-xL,gene
R7233 T9586 T9584 punct ",",gene
R7234 T9587 T9590 nsubjpass which,regulated
R7235 T9588 T9590 auxpass is,regulated
R7236 T9589 T9590 advmod also,regulated
R7237 T9590 T9584 relcl regulated,gene
R7238 T9591 T9590 agent by,regulated
R7239 T9592 T9591 pobj NF-κB,by
R724 T1096 T1092 conj metastasis,sclerosis
R7240 T9593 T9578 punct .,examined
R7241 T9594 T9595 mark As,shown
R7242 T9595 T9601 advcl shown,reduced
R7243 T9596 T9595 prep in,shown
R7244 T9597 T9598 compound Figure,6C
R7245 T9598 T9596 pobj 6C,in
R7246 T9599 T9601 punct ",",reduced
R7247 T9600 T9601 nsubj Ro-31-8220,reduced
R7248 T9601 T9601 ROOT reduced,reduced
R7249 T9602 T9604 nmod M-CSF-stimulated,expression
R725 T1097 T1099 nmod [,]
R7250 T9603 T9604 amod BCL-xL,expression
R7251 T9604 T9601 dobj expression,reduced
R7252 T9605 T9601 prep compared,reduced
R7253 T9606 T9605 prep to,compared
R7254 T9607 T9606 pobj cells,to
R7255 T9608 T9607 acl treated,cells
R7256 T9609 T9608 prep with,treated
R7257 T9610 T9609 pobj M-CSF,with
R7258 T9611 T9610 cc and,M-CSF
R7259 T9612 T9614 det the,control
R726 T1098 T1099 nummod 4,]
R7260 T9613 T9614 compound vehicle,control
R7261 T9614 T9610 conj control,M-CSF
R7262 T9615 T9614 appos DMSO,control
R7263 T9616 T9618 punct (,<
R7264 T9617 T9618 compound p,<
R7265 T9618 T9619 compound <,0.05
R7266 T9619 T9614 appos 0.05,control
R7267 T9620 T9614 punct ),control
R7268 T9621 T9601 punct .,reduced
R727 T1099 T1088 conj ],atherosclerosis
R728 T1100 T1099 punct ",",]
R729 T1101 T1103 nmod [,]
R73 T105 T101 pobj survival,in
R730 T1102 T1103 nummod 5,]
R731 T1103 T1099 conj ],]
R732 T1104 T1103 punct ",",]
R733 T1105 T1107 nmod [,]
R734 T1106 T1107 nummod 6,]
R735 T1107 T1103 conj ],]
R736 T1108 T1079 punct .,associated
R737 T1109 T1111 nsubj We,identified
R738 T1110 T1111 advmod previously,identified
R739 T1111 T1111 ROOT identified,identified
R74 T106 T94 cc and,is
R740 T1112 T1115 mark that,is
R741 T1113 T1114 amod NF-κB,activation
R742 T1114 T1115 nsubj activation,is
R743 T1115 T1111 ccomp is,identified
R744 T1116 T1115 acomp important,is
R745 T1117 T1116 prep in,important
R746 T1118 T1120 amod M-CSF-induced,survival
R747 T1119 T1120 compound monocyte,survival
R748 T1120 T1117 pobj survival,in
R749 T1121 T1123 nmod [,]
R75 T107 T108 det this,study
R750 T1122 T1123 nummod 7,]
R751 T1123 T1115 npadvmod ],is
R752 T1124 T1111 punct .,identified
R753 T1125 T1139 prep In,regulates
R754 T1126 T1125 pobj addition,In
R755 T1127 T1126 prep to,addition
R756 T1128 T1129 poss its,role
R757 T1129 T1127 pobj role,to
R758 T1130 T1129 prep in,role
R759 T1131 T1133 nmod mononuclear,survival
R76 T108 T109 nsubj study,focused
R760 T1132 T1133 amod phagocyte,survival
R761 T1133 T1130 pobj survival,in
R762 T1134 T1129 punct ",",role
R7626 T10239 T10276 nsubj Identification,suggested
R7627 T10240 T10239 prep of,Identification
R7628 T10241 T10240 pobj PKCα,of
R7629 T10242 T10239 prep as,Identification
R763 T1135 T1137 det the,factor
R7630 T10243 T10245 det the,Activator
R7631 T10244 T10245 compound Upstream,Activator
R7632 T10245 T10242 pobj Activator,as
R7633 T10246 T10245 prep of,Activator
R7634 T10247 T10246 pobj NF-κB,of
R7635 T10248 T10245 prep in,Activator
R7636 T10249 T10250 compound Myeloid,Cells
R7637 T10250 T10248 pobj Cells,in
R7638 T10251 T10256 advmod Even,consists
R7639 T10252 T10256 mark though,consists
R764 T1136 T1137 compound transcription,factor
R7640 T10253 T10255 det the,family
R7641 T10254 T10255 compound PKC,family
R7642 T10255 T10256 nsubj family,consists
R7643 T10256 T10276 advcl consists,suggested
R7644 T10257 T10256 prep of,consists
R7645 T10258 T10259 nummod 10,members
R7646 T10259 T10257 pobj members,of
R7647 T10260 T10256 punct ",",consists
R7648 T10261 T10256 advcl finding,consists
R7649 T10262 T10265 mark that,β
R765 T1137 T1139 nsubj factor,regulates
R7650 T10263 T10265 nsubj PKCα,β
R7651 T10264 T10265 compound /,β
R7652 T10265 T10261 ccomp β,finding
R7653 T10266 T10265 dobj inhibitors,β
R7654 T10267 T10266 cc and,inhibitors
R7655 T10268 T10270 amod intracellular,inhibitors
R7656 T10269 T10270 compound calcium,inhibitors
R7657 T10270 T10266 conj inhibitors,inhibitors
R7658 T10271 T10265 conj reduced,β
R7659 T10272 T10274 amod M-CSF-induced,activity
R766 T1138 T1139 nsubj NF-κB,regulates
R7660 T10273 T10274 compound NF-κB,activity
R7661 T10274 T10271 dobj activity,reduced
R7662 T10275 T10276 punct ",",suggested
R7663 T10276 T10276 ROOT suggested,suggested
R7664 T10277 T10279 nsubjpass PKCα,involved
R7665 T10278 T10279 auxpass was,involved
R7666 T10279 T10276 ccomp involved,suggested
R7667 T10280 T10279 prep in,involved
R7668 T10281 T10282 amod NF-κB,activation
R7669 T10282 T10280 pobj activation,in
R767 T1139 T1139 ROOT regulates,regulates
R7670 T10283 T10282 prep after,activation
R7671 T10284 T10285 compound M-CSF,treatment
R7672 T10285 T10283 pobj treatment,after
R7673 T10286 T10276 punct .,suggested
R7674 T10287 T10288 aux To,confirm
R7675 T10288 T10316 advcl confirm,co-transfected
R7676 T10289 T10290 det the,role
R7677 T10290 T10288 dobj role,confirm
R7678 T10291 T10290 prep of,role
R7679 T10292 T10291 pobj PKCα,of
R768 T1140 T1141 amod numerous,genes
R7680 T10293 T10292 prep in,PKCα
R7681 T10294 T10295 compound NF-κB,activation
R7682 T10295 T10293 pobj activation,in
R7683 T10296 T10295 prep in,activation
R7684 T10297 T10296 pobj macrophages,in
R7685 T10298 T10290 punct ",",role
R7686 T10299 T10290 conj constructs,role
R7687 T10300 T10290 prep for,role
R7688 T10301 T10302 det either,wildtype
R7689 T10302 T10300 pobj wildtype,for
R769 T1141 T1139 dobj genes,regulates
R7690 T10303 T10302 punct (,wildtype
R7691 T10304 T10302 appos WT,wildtype
R7692 T10305 T10304 punct ),WT
R7693 T10306 T10302 punct -,wildtype
R7694 T10307 T10302 conj PKCα,wildtype
R7695 T10308 T10307 cc or,PKCα
R7696 T10309 T10307 conj kinase-deficient,PKCα
R7697 T10310 T10311 punct (,KD
R7698 T10311 T10302 appos KD,wildtype
R7699 T10312 T10311 punct ),KD
R77 T109 T94 conj focused,is
R770 T1142 T1143 nsubj that,play
R7700 T10313 T10314 punct -,PKCα
R7701 T10314 T10315 nsubj PKCα,was
R7702 T10315 T10316 auxpass was,co-transfected
R7703 T10316 T10316 ROOT co-transfected,co-transfected
R7704 T10317 T10316 prep with,co-transfected
R7705 T10318 T10321 det the,gene
R7706 T10319 T10321 amod pNF-κB-SEAP,gene
R7707 T10320 T10321 compound reporter,gene
R7708 T10321 T10317 pobj gene,with
R7709 T10322 T10321 cc and,gene
R771 T1143 T1141 relcl play,genes
R7710 T10323 T10324 compound SEAP,secretion
R7711 T10324 T10321 conj secretion,gene
R7712 T10325 T10326 auxpass was,measured
R7713 T10326 T10316 conj measured,co-transfected
R7714 T10327 T10316 punct .,co-transfected
R7715 T10328 T10329 mark As,shown
R7716 T10329 T10335 advcl shown,co-transfected
R7717 T10330 T10329 prep in,shown
R7718 T10331 T10332 compound Figure,7A
R7719 T10332 T10330 pobj 7A,in
R772 T1144 T1145 amod important,roles
R7720 T10333 T10335 punct ",",co-transfected
R7721 T10334 T10335 nsubj MDMs,co-transfected
R7722 T10335 T10335 ROOT co-transfected,co-transfected
R7723 T10336 T10335 prep with,co-transfected
R7724 T10337 T10336 pobj pNF-κB-SEAP,with
R7725 T10338 T10337 cc and,pNF-κB-SEAP
R7726 T10339 T10337 conj WT-PKCα,pNF-κB-SEAP
R7727 T10340 T10335 conj had,co-transfected
R7728 T10341 T10343 det a,increase
R7729 T10342 T10343 amod 1.8-fold,increase
R773 T1145 T1143 dobj roles,play
R7730 T10343 T10340 dobj increase,had
R7731 T10344 T10343 prep in,increase
R7732 T10345 T10347 amod NF-κB,activity
R7733 T10346 T10347 amod transcriptional,activity
R7734 T10347 T10344 pobj activity,in
R7735 T10348 T10343 prep after,increase
R7736 T10349 T10350 compound M-CSF,activation
R7737 T10350 T10348 pobj activation,after
R7738 T10351 T10343 prep compared,increase
R7739 T10352 T10351 prep with,compared
R774 T1146 T1143 prep in,play
R7740 T10353 T10354 compound NS,cells
R7741 T10354 T10352 pobj cells,with
R7742 T10355 T10354 punct (,cells
R7743 T10356 T10354 appos p,cells
R7744 T10357 T10358 punct =,0.05
R7745 T10358 T10356 prep 0.05,p
R7746 T10359 T10354 punct ),cells
R7747 T10360 T10354 punct ",",cells
R7748 T10361 T10354 amod similar,cells
R7749 T10362 T10361 prep to,similar
R775 T1147 T1148 amod cellular,signaling
R7750 T10363 T10364 amod M-CSF-treated,cells
R7751 T10364 T10362 pobj cells,to
R7752 T10365 T10364 acl expressing,cells
R7753 T10366 T10367 advmod only,pNF-κB-SEAP
R7754 T10367 T10365 dobj pNF-κB-SEAP,expressing
R7755 T10368 T10335 punct .,co-transfected
R7756 T10369 T10369 ROOT Transfecting,Transfecting
R7757 T10370 T10371 amod human,macrophages
R7758 T10371 T10369 dobj macrophages,Transfecting
R7759 T10372 T10369 prep with,Transfecting
R776 T1148 T1146 pobj signaling,in
R7760 T10373 T10374 det the,KD-PKCα
R7761 T10374 T10372 pobj KD-PKCα,with
R7762 T10375 T10369 nsubj construct,Transfecting
R7763 T10376 T10377 advmod significantly,reduced
R7764 T10377 T10380 amod reduced,activity
R7765 T10378 T10380 amod M-CSF-induced,activity
R7766 T10379 T10380 compound NF-κB,activity
R7767 T10380 T10375 dobj activity,construct
R7768 T10381 T10375 prep compared,construct
R7769 T10382 T10381 prep to,compared
R777 T1149 T1148 punct ",",signaling
R7770 T10383 T10385 amod WT-PKCα,cells
R7771 T10384 T10385 amod transfected,cells
R7772 T10385 T10382 pobj cells,to
R7773 T10386 T10385 punct (,cells
R7774 T10387 T10385 appos p,cells
R7775 T10388 T10389 punct =,0.016
R7776 T10389 T10387 pobj 0.016,p
R7777 T10390 T10385 punct ),cells
R7778 T10391 T10369 punct .,Transfecting
R7779 T10392 T10397 advmod Similarly,transfected
R778 T1150 T1151 compound stress,response
R7780 T10393 T10397 punct ",",transfected
R7781 T10394 T10397 nsubj RAW,transfected
R7782 T10395 T10396 nummod 264.7,cells
R7783 T10396 T10397 compound cells,transfected
R7784 T10397 T10397 ROOT transfected,transfected
R7785 T10398 T10397 prep with,transfected
R7786 T10399 T10398 pobj WT-PKCα,with
R7787 T10400 T10397 conj had,transfected
R7788 T10401 T10405 nummod 2.5-fold,activity
R7789 T10402 T10401 amod more,2.5-fold
R779 T1151 T1148 conj response,signaling
R7790 T10403 T10405 amod NF-κB,activity
R7791 T10404 T10405 compound transcriptional,activity
R7792 T10405 T10400 dobj activity,had
R7793 T10406 T10400 prep after,had
R7794 T10407 T10408 compound M-CSF,activation
R7795 T10408 T10406 pobj activation,after
R7796 T10409 T10400 prep compared,had
R7797 T10410 T10409 prep to,compared
R7798 T10411 T10412 compound unstimulated,RAW
R7799 T10412 T10410 pobj RAW,to
R78 T110 T109 prep at,focused
R780 T1152 T1151 punct ",",response
R7800 T10413 T10414 nummod 264.7,cells
R7801 T10414 T10412 dobj cells,RAW
R7802 T10415 T10416 punct (,NS
R7803 T10416 T10414 appos NS,cells
R7804 T10417 T10400 punct ),had
R7805 T10418 T10418 ROOT transfected,transfected
R7806 T10419 T10418 prep with,transfected
R7807 T10420 T10419 pobj WT-PKCα,with
R7808 T10421 T10423 punct (,7B
R7809 T10422 T10423 compound Figure,7B
R781 T1153 T1154 compound cell,growth
R7810 T10423 T10420 appos 7B,WT-PKCα
R7811 T10424 T10423 punct ),7B
R7812 T10425 T10418 punct .,transfected
R7813 T10426 T10430 nsubj Expression,construct
R7814 T10427 T10426 prep of,Expression
R7815 T10428 T10429 det the,KD-PKCα
R7816 T10429 T10427 pobj KD-PKCα,of
R7817 T10430 T10430 ROOT construct,construct
R7818 T10431 T10430 prep into,construct
R7819 T10432 T10431 pobj RAW,into
R782 T1154 T1151 conj growth,response
R7820 T10433 T10434 nummod 264.7,cells
R7821 T10434 T10446 nsubj cells,compared
R7822 T10435 T10434 acl reduced,cells
R7823 T10436 T10438 amod M-CSF-induced,activity
R7824 T10437 T10438 compound NF-κB,activity
R7825 T10438 T10435 dobj activity,reduced
R7826 T10439 T10435 prep to,reduced
R7827 T10440 T10439 pobj 1.5-fold,to
R7828 T10441 T10440 punct (,1.5-fold
R7829 T10442 T10440 appos p,1.5-fold
R783 T1155 T1154 punct ",",growth
R7830 T10443 T10444 punct =,0.045
R7831 T10444 T10442 nummod 0.045,p
R7832 T10445 T10440 punct ),1.5-fold
R7833 T10446 T10430 prep compared,construct
R7834 T10447 T10446 prep to,compared
R7835 T10448 T10447 pobj cells,to
R7836 T10449 T10448 acl transfected,cells
R7837 T10450 T10449 prep with,transfected
R7838 T10451 T10452 compound WT,PKCα
R7839 T10452 T10450 pobj PKCα,with
R784 T1156 T1154 conj survival,growth
R7840 T10453 T10430 punct .,construct
R7841 T10454 T10455 compound Cell,survival
R7842 T10455 T10458 nsubjpass survival,examined
R7843 T10456 T10458 auxpass was,examined
R7844 T10457 T10458 advmod also,examined
R7845 T10458 T10458 ROOT examined,examined
R7846 T10459 T10458 prep in,examined
R7847 T10460 T10459 pobj MDMs,in
R7848 T10461 T10460 acl expressing,MDMs
R7849 T10462 T10463 preconj either,WT-PKCα
R785 T1157 T1156 punct ",",survival
R7850 T10463 T10461 dobj WT-PKCα,expressing
R7851 T10464 T10463 cc or,WT-PKCα
R7852 T10465 T10463 conj KD-PKCα,WT-PKCα
R7853 T10466 T10463 conj constructs,WT-PKCα
R7854 T10467 T10461 prep by,expressing
R7855 T10468 T10469 compound Annexin,V-FITC
R7856 T10469 T10467 pobj V-FITC,by
R7857 T10470 T10469 cc and,V-FITC
R7858 T10471 T10469 conj PI,V-FITC
R7859 T10472 T10458 advcl staining,examined
R786 T1158 T1156 conj differentiation,survival
R7860 T10473 T10458 punct .,examined
R7861 T10474 T10475 mark As,expected
R7862 T10475 T10478 advcl expected,increased
R7863 T10476 T10478 punct ",",increased
R7864 T10477 T10478 nsubj M-CSF,increased
R7865 T10478 T10478 ROOT increased,increased
R7866 T10479 T10480 compound MDM,survival
R7867 T10480 T10478 dobj survival,increased
R7868 T10481 T10482 mark as,measured
R7869 T10482 T10478 advcl measured,increased
R787 T1159 T1158 cc and,differentiation
R7870 T10483 T10482 agent by,measured
R7871 T10484 T10485 det the,percent
R7872 T10485 T10483 pobj percent,by
R7873 T10486 T10485 prep of,percent
R7874 T10487 T10488 compound Annexin,V/PI
R7875 T10488 T10490 nmod V/PI,cells
R7876 T10489 T10490 amod negative,cells
R7877 T10490 T10486 pobj cells,of
R7878 T10491 T10478 punct .,increased
R7879 T10492 T10494 advmod Similarly,expression
R788 T1160 T1158 conj inflammation,differentiation
R7880 T10493 T10494 punct ",",expression
R7881 T10494 T10494 ROOT expression,expression
R7882 T10495 T10494 prep of,expression
R7883 T10496 T10498 amod WT-PKCα,cells
R7884 T10497 T10498 amod protected,cells
R7885 T10498 T10495 pobj cells,of
R7886 T10499 T10498 prep from,cells
R7887 T10500 T10499 pobj apoptosis,from
R7888 T10501 T10494 punct .,expression
R7889 T10502 T10508 prep In,decreased
R789 T1161 T1158 conj [,differentiation
R7890 T10503 T10502 pobj contrast,In
R7891 T10504 T10508 punct ",",decreased
R7892 T10505 T10508 nsubj expression,decreased
R7893 T10506 T10505 prep of,expression
R7894 T10507 T10506 pobj KD-PKCα,of
R7895 T10508 T10508 ROOT decreased,decreased
R7896 T10509 T10511 amod M-CSF-induced,survival
R7897 T10510 T10511 compound cell,survival
R7898 T10511 T10508 dobj survival,decreased
R7899 T10512 T10511 punct (,survival
R79 T111 T110 pcomp identifying,at
R790 T1162 T1163 nummod 8,]
R7900 T10513 T10514 advmod p,<
R7901 T10514 T10515 nmod <,0.01
R7902 T10515 T10511 appos 0.01,survival
R7903 T10516 T10511 punct ),survival
R7904 T10517 T10519 punct (,7C
R7905 T10518 T10519 compound Figure,7C
R7906 T10519 T10511 appos 7C,survival
R7907 T10520 T10508 punct ),decreased
R7908 T10521 T10508 punct .,decreased
R7909 T10522 T10525 advmod Next,examined
R791 T1163 T1139 npadvmod ],regulates
R7910 T10523 T10525 punct ",",examined
R7911 T10524 T10525 nsubj we,examined
R7912 T10525 T10525 ROOT examined,examined
R7913 T10526 T10527 det the,effect
R7914 T10527 T10525 dobj effect,examined
R7915 T10528 T10527 prep of,effect
R7916 T10529 T10528 pcomp expressing,of
R7917 T10530 T10532 det the,constructs
R7918 T10531 T10532 nsubj PKCα,constructs
R7919 T10532 T10529 dobj constructs,expressing
R792 T1164 T1139 punct .,regulates
R7920 T10533 T10532 prep on,constructs
R7921 T10534 T10535 amod NF-κB,phosphorylation
R7922 T10535 T10533 pobj phosphorylation,on
R7923 T10536 T10525 punct .,examined
R7924 T10537 T10538 mark As,shown
R7925 T10538 T10552 advcl shown,affect
R7926 T10539 T10538 prep in,shown
R7927 T10540 T10539 pobj Figure,in
R7928 T10541 T10540 nummod 7D,Figure
R7929 T10542 T10540 punct ",",Figure
R793 T1165 T1166 expl There,are
R7930 T10543 T10538 dobj expression,shown
R7931 T10544 T10543 prep of,expression
R7932 T10545 T10544 pobj KD-PKCα,of
R7933 T10546 T10545 prep in,KD-PKCα
R7934 T10547 T10546 pobj RAW,in
R7935 T10548 T10549 nummod 264.7,cells
R7936 T10549 T10544 pobj cells,of
R7937 T10550 T10552 aux did,affect
R7938 T10551 T10552 neg not,affect
R7939 T10552 T10552 ROOT affect,affect
R794 T1166 T1166 ROOT are,are
R7940 T10553 T10555 det the,phosphorylation
R7941 T10554 T10555 amod constitutive,phosphorylation
R7942 T10555 T10552 dobj phosphorylation,affect
R7943 T10556 T10555 prep at,phosphorylation
R7944 T10557 T10556 pobj Ser536,at
R7945 T10558 T10557 prep of,Ser536
R7946 T10559 T10558 pobj NF-κB,of
R7947 T10560 T10559 nummod p65,NF-κB
R7948 T10561 T10552 punct ",",affect
R7949 T10562 T10552 cc but,affect
R795 T1167 T1168 nummod five,members
R7950 T10563 T10552 conj attenuated,affect
R7951 T10564 T10565 det the,phosphorylation
R7952 T10565 T10563 dobj phosphorylation,attenuated
R7953 T10566 T10563 prep at,attenuated
R7954 T10567 T10566 pobj Ser276,at
R7955 T10568 T10552 punct .,affect
R7956 T10569 T10574 nsubj Expression,effect
R7957 T10570 T10569 prep of,Expression
R7958 T10571 T10570 pobj WT-PKCα,of
R7959 T10572 T10574 aux did,effect
R796 T1168 T1166 attr members,are
R7960 T10573 T10574 neg not,effect
R7961 T10574 T10574 ROOT effect,effect
R7962 T10575 T10576 det the,phosphorylation
R7963 T10576 T10574 dobj phosphorylation,effect
R7964 T10577 T10576 prep of,phosphorylation
R7965 T10578 T10579 det either,residue
R7966 T10579 T10577 pobj residue,of
R7967 T10580 T10576 prep with,phosphorylation
R7968 T10581 T10580 cc or,with
R7969 T10582 T10580 conj without,with
R797 T1169 T1168 prep in,members
R7970 T10583 T10584 compound M-CSF,stimulation
R7971 T10584 T10582 pobj stimulation,without
R7972 T10585 T10574 punct .,effect
R7973 T10586 T10587 det These,observations
R7974 T10587 T10588 nsubj observations,demonstrate
R7975 T10588 T10588 ROOT demonstrate,demonstrate
R7976 T10589 T10591 mark that,is
R7977 T10590 T10591 nsubj PKCα,is
R7978 T10591 T10588 ccomp is,demonstrate
R7979 T10592 T10591 acomp important,is
R798 T1170 T1172 det the,family
R7980 T10593 T10592 prep in,important
R7981 T10594 T10596 amod M-CSF-regulated,survival
R7982 T10595 T10596 compound cell,survival
R7983 T10596 T10593 pobj survival,in
R7984 T10597 T10596 cc and,survival
R7985 T10598 T10599 amod NF-κB,activation
R7986 T10599 T10596 conj activation,survival
R7987 T10600 T10599 cc and,activation
R7988 T10601 T10602 advmod likely,regulated
R7989 T10602 T10591 conj regulated,is
R799 T1171 T1172 compound NF-κB,family
R7990 T10603 T10602 prep through,regulated
R7991 T10604 T10603 pobj phosphorylation,through
R7992 T10605 T10604 prep of,phosphorylation
R7993 T10606 T10605 pobj Ser276,of
R7994 T10607 T10606 prep of,Ser276
R7995 T10608 T10609 compound NF-κB,p65
R7996 T10609 T10607 pobj p65,of
R7997 T10610 T10588 punct .,demonstrate
R7998 T10611 T10613 aux To,validate
R7999 T10612 T10613 advmod further,validate
R80 T112 T113 det the,mechanism
R800 T1172 T1169 pobj family,in
R8000 T10613 T10627 advcl validate,employed
R8001 T10614 T10615 det the,impact
R8002 T10615 T10613 dobj impact,validate
R8003 T10616 T10618 dobj that,played
R8004 T10617 T10618 nsubj PKCα,played
R8005 T10618 T10615 relcl played,impact
R8006 T10619 T10618 prep in,played
R8007 T10620 T10623 amod M-CSF-induced,activity
R8008 T10621 T10623 nmod NF-κB,activity
R8009 T10622 T10623 compound transcriptional,activity
R801 T1173 T1168 punct :,members
R8010 T10623 T10619 pobj activity,in
R8011 T10624 T10627 punct ",",employed
R8012 T10625 T10627 nsubj we,employed
R8013 T10626 T10627 advmod next,employed
R8014 T10627 T10627 ROOT employed,employed
R8015 T10628 T10629 compound PKCα,siRNA
R8016 T10629 T10630 compound siRNA,treatment
R8017 T10630 T10627 dobj treatment,employed
R8018 T10631 T10630 prep of,treatment
R8019 T10632 T10635 nmod MDM,cells
R802 T1174 T1168 appos RelA/p65,members
R8020 T10633 T10632 cc or,MDM
R8021 T10634 T10632 conj RAW,MDM
R8022 T10635 T10631 pobj cells,of
R8023 T10636 T10627 punct .,employed
R8024 T10637 T10638 det A,pool
R8025 T10638 T10644 nsubjpass pool,transfected
R8026 T10639 T10638 prep of,pool
R8027 T10640 T10642 amod specific,siRNA
R8028 T10641 T10642 compound PKCα,siRNA
R8029 T10642 T10639 pobj siRNA,of
R803 T1175 T1174 punct ",",RelA/p65
R8030 T10643 T10644 auxpass were,transfected
R8031 T10644 T10644 ROOT transfected,transfected
R8032 T10645 T10644 prep into,transfected
R8033 T10646 T10645 pobj MDM,into
R8034 T10647 T10646 cc or,MDM
R8035 T10648 T10646 conj Raw,MDM
R8036 T10649 T10650 nummod 264.7,cells
R8037 T10650 T10644 conj cells,transfected
R8038 T10651 T10650 prep in,cells
R8039 T10652 T10653 det the,presence
R804 T1176 T1174 appos p50,RelA/p65
R8040 T10653 T10651 pobj presence,in
R8041 T10654 T10653 cc or,presence
R8042 T10655 T10653 conj absence,presence
R8043 T10656 T10655 cc or,absence
R8044 T10657 T10655 conj M-CSF,absence
R8045 T10658 T10644 punct .,transfected
R8046 T10659 T10663 advcl Reducing,decreased
R8047 T10660 T10662 amod native,expression
R8048 T10661 T10662 compound PKCα,expression
R8049 T10662 T10659 dobj expression,Reducing
R805 T1177 T1176 punct ",",p50
R8050 T10663 T10663 ROOT decreased,decreased
R8051 T10664 T10667 amod M-CSF-induced,activity
R8052 T10665 T10667 nmod NF-κB,activity
R8053 T10666 T10667 compound transcriptional,activity
R8054 T10667 T10663 dobj activity,decreased
R8055 T10668 T10663 prep in,decreased
R8056 T10669 T10673 det both,7E
R8057 T10670 T10673 compound MDM,7E
R8058 T10671 T10672 punct (,Figure
R8059 T10672 T10673 compound Figure,7E
R806 T1178 T1176 appos p52,p50
R8060 T10673 T10668 pobj 7E,in
R8061 T10674 T10663 punct ),decreased
R8062 T10675 T10676 punct (,p
R8063 T10676 T10676 ROOT p,p
R8064 T10677 T10678 punct =,0.012
R8065 T10678 T10676 prep 0.012,p
R8066 T10679 T10676 punct ),p
R8067 T10680 T10676 cc and,p
R8068 T10681 T10683 nmod Raw,cells
R8069 T10682 T10683 nummod 264.7,cells
R807 T1179 T1176 punct ",",p50
R8070 T10683 T10689 nmod cells,p
R8071 T10684 T10685 punct (,Figure
R8072 T10685 T10683 appos Figure,cells
R8073 T10686 T10685 nummod 7F,Figure
R8074 T10687 T10683 punct ),cells
R8075 T10688 T10683 punct (,cells
R8076 T10689 T10676 conj p,p
R8077 T10690 T10691 punct =,0.01
R8078 T10691 T10689 prep 0.01,p
R8079 T10692 T10689 punct ),p
R808 T1180 T1176 conj c-Rel,p50
R8080 T10693 T10663 punct .,decreased
R8081 T10694 T10696 nsubj We,examined
R8082 T10695 T10696 advmod also,examined
R8083 T10696 T10696 ROOT examined,examined
R8084 T10697 T10698 compound cell,survival
R8085 T10698 T10696 dobj survival,examined
R8086 T10699 T10698 prep of,survival
R8087 T10700 T10701 det the,MDMs
R8088 T10701 T10699 pobj MDMs,of
R8089 T10702 T10696 prep by,examined
R809 T1181 T1180 cc and,c-Rel
R8090 T10703 T10704 compound Annexin,V-FITC
R8091 T10704 T10702 pobj V-FITC,by
R8092 T10705 T10704 cc and,V-FITC
R8093 T10706 T10704 conj PI,V-FITC
R8094 T10707 T10696 advcl staining,examined
R8095 T10708 T10707 advmod after,staining
R8096 T10709 T10711 compound PKCα,transfection
R8097 T10710 T10711 compound siRNA,transfection
R8098 T10711 T10708 pobj transfection,after
R8099 T10712 T10696 punct .,examined
R81 T113 T111 dobj mechanism,identifying
R810 T1182 T1180 conj RelB,c-Rel
R8100 T10713 T10714 mark As,shown
R8101 T10714 T10714 ROOT shown,shown
R8102 T10715 T10714 prep in,shown
R8103 T10716 T10721 nmod Figure,survival
R8104 T10717 T10716 nummod 7G,Figure
R8105 T10718 T10716 punct ",",Figure
R8106 T10719 T10721 amod M-CSF-induced,survival
R8107 T10720 T10721 compound MDM,survival
R8108 T10721 T10715 pobj survival,in
R8109 T10722 T10723 auxpass was,reduced
R811 T1183 T1166 punct .,are
R8110 T10723 T10714 conj reduced,shown
R8111 T10724 T10723 prep in,reduced
R8112 T10725 T10726 det the,cells
R8113 T10726 T10724 pobj cells,in
R8114 T10727 T10723 conj transfected,reduced
R8115 T10728 T10727 prep with,transfected
R8116 T10729 T10730 compound PKCα,siRNA
R8117 T10730 T10728 pobj siRNA,with
R8118 T10731 T10727 prep compared,transfected
R8119 T10732 T10731 prep with,compared
R812 T1184 T1188 det The,complex
R8120 T10733 T10732 pobj cells,with
R8121 T10734 T10733 acl transfected,cells
R8122 T10735 T10734 prep with,transfected
R8123 T10736 T10737 compound control,siRNA
R8124 T10737 T10735 pobj siRNA,with
R8125 T10738 T10737 punct (,siRNA
R8126 T10739 T10737 parataxis p,siRNA
R8127 T10740 T10741 punct =,0.047
R8128 T10741 T10739 prep 0.047,p
R8129 T10742 T10737 punct ),siRNA
R813 T1185 T1186 advmod most,common
R8130 T10743 T10714 punct .,shown
R8131 T10744 T10749 prep In,confirmed
R8132 T10745 T10744 pobj Figure,In
R8133 T10746 T10745 nummod 7H,Figure
R8134 T10747 T10749 punct ",",confirmed
R8135 T10748 T10749 nsubj we,confirmed
R8136 T10749 T10749 ROOT confirmed,confirmed
R8137 T10750 T10754 mark that,decreased
R8138 T10751 T10753 compound PKCα,transfection
R8139 T10752 T10753 compound siRNA,transfection
R814 T1186 T1188 amod common,complex
R8140 T10753 T10754 nsubj transfection,decreased
R8141 T10754 T10749 ccomp decreased,confirmed
R8142 T10755 T10757 compound PKCα,expression
R8143 T10756 T10757 compound protein,expression
R8144 T10757 T10754 dobj expression,decreased
R8145 T10758 T10754 prep in,decreased
R8146 T10759 T10760 det both,MDM
R8147 T10760 T10764 nmod MDM,cells
R8148 T10761 T10760 cc and,MDM
R8149 T10762 T10760 conj Raw,MDM
R815 T1187 T1188 compound activating,complex
R8150 T10763 T10764 nummod 264.7,cells
R8151 T10764 T10758 pobj cells,in
R8152 T10765 T10749 punct .,confirmed
R8153 T10766 T10767 poss Our,results
R8154 T10767 T10768 nsubj results,indicated
R8155 T10768 T10768 ROOT indicated,indicated
R8156 T10769 T10771 mark that,regulated
R8157 T10770 T10771 nsubj PKCα,regulated
R8158 T10771 T10768 ccomp regulated,indicated
R8159 T10772 T10773 compound NF-κB,activation
R816 T1188 T1189 nsubj complex,is
R8160 T10773 T10771 dobj activation,regulated
R8161 T10774 T10773 cc and,activation
R8162 T10775 T10777 amod M-CSF-regulated,survival
R8163 T10776 T10777 compound cell,survival
R8164 T10777 T10773 conj survival,activation
R8165 T10778 T10768 punct .,indicated
R817 T1189 T1189 ROOT is,is
R818 T1190 T1192 det the,heterodimer
R819 T1191 T1192 nummod p50/p65,heterodimer
R82 T114 T113 prep of,mechanism
R820 T1192 T1189 attr heterodimer,is
R821 T1193 T1192 punct ",",heterodimer
R822 T1194 T1192 acl driven,heterodimer
R823 T1195 T1194 agent by,driven
R824 T1196 T1198 det the,domain
R825 T1197 T1198 compound activation,domain
R826 T1198 T1195 pobj domain,by
R827 T1199 T1198 prep in,domain
R828 T1200 T1203 det the,subunit
R829 T1201 T1203 nmod NF-κB,subunit
R83 T115 T117 amod NF-κB,activation
R830 T1202 T1203 nummod p65,subunit
R831 T1203 T1199 pobj subunit,in
R832 T1204 T1189 punct .,is
R833 T1205 T1207 nsubj NF-κB,regulates
R834 T1206 T1205 nummod p65,NF-κB
R835 T1207 T1207 ROOT regulates,regulates
R836 T1208 T1210 amod important,processes
R837 T1209 T1210 amod developmental,processes
R838 T1210 T1207 dobj processes,regulates
R839 T1211 T1213 nmod [,]
R84 T116 T117 amod transcriptional,activation
R840 T1212 T1213 nummod 9,]
R841 T1213 T1217 nmod ],]
R842 T1214 T1213 punct ",",]
R843 T1215 T1217 nmod [,]
R844 T1216 T1217 nummod 10,]
R845 T1217 T1207 npadvmod ],regulates
R846 T1218 T1207 punct .,regulates
R847 T1219 T1219 ROOT Mice,Mice
R848 T1220 T1219 acl lacking,Mice
R849 T1221 T1223 nsubj NF-κB,die
R85 T117 T114 pobj activation,of
R850 T1222 T1223 nummod p65,die
R851 T1223 T1220 dep die,lacking
R852 T1224 T1223 prep in,die
R853 T1225 T1224 pobj utero,in
R854 T1226 T1223 cc and,die
R855 T1227 T1223 conj have,die
R856 T1228 T1230 amod extensive,damage
R857 T1229 T1230 compound liver,damage
R858 T1230 T1227 dobj damage,have
R859 T1231 T1230 prep via,damage
R86 T118 T109 punct .,focused
R860 T1232 T1234 amod enhanced,[
R861 T1233 T1234 compound apoptosis,[
R862 T1234 T1231 pobj [,via
R863 T1235 T1236 nummod 9,]
R864 T1236 T1230 appos ],damage
R865 T1237 T1220 punct .,lacking
R866 T1238 T1239 amod Embryonic,macrophages
R867 T1239 T1245 nsubj macrophages,are
R868 T1240 T1239 prep from,macrophages
R869 T1241 T1244 nmod NF-κB,mice
R87 T119 T122 advmod Here,demonstrate
R870 T1242 T1241 nummod p65,NF-κB
R871 T1243 T1244 amod null,mice
R872 T1244 T1240 pobj mice,from
R873 T1245 T1245 ROOT are,are
R874 T1246 T1245 acomp susceptible,are
R875 T1247 T1246 prep to,susceptible
R876 T1248 T1249 amod TNFα-induced,apoptosis
R877 T1249 T1247 pobj apoptosis,to
R878 T1250 T1252 nsubjpass which,rescued
R879 T1251 T1252 auxpass is,rescued
R88 T120 T122 punct ",",demonstrate
R880 T1252 T1249 relcl rescued,apoptosis
R881 T1253 T1252 agent by,rescued
R882 T1254 T1253 pcomp overexpressing,by
R883 T1255 T1259 det the,[
R884 T1256 T1259 nmod NF-κB,[
R885 T1257 T1256 nummod p65,NF-κB
R886 T1258 T1259 amod subunit,[
R887 T1259 T1254 dobj [,overexpressing
R888 T1260 T1261 nummod 10,]
R889 T1261 T1254 dobj ],overexpressing
R89 T121 T122 nsubj we,demonstrate
R890 T1262 T1245 punct .,are
R891 T1263 T1267 advmod Moreover,induces
R892 T1264 T1267 punct ",",induces
R893 T1265 T1267 csubj inhibiting,induces
R894 T1266 T1267 nsubj NF-κB,induces
R895 T1267 T1267 ROOT induces,induces
R896 T1268 T1269 compound cell,death
R897 T1269 T1267 dobj death,induces
R898 T1270 T1267 prep in,induces
R899 T1271 T1273 amod many,types
R90 T122 T122 ROOT demonstrate,demonstrate
R900 T1272 T1273 compound cell,types
R901 T1273 T1270 pobj types,in
R902 T1274 T1273 cc and,types
R903 T1275 T1276 amod cytokine-independent,survival
R904 T1276 T1273 conj survival,types
R905 T1277 T1278 auxpass is,mediated
R906 T1278 T1267 conj mediated,induces
R907 T1279 T1278 agent by,mediated
R908 T1280 T1281 advmod constitutively,active
R909 T1281 T1282 amod active,NF-κB
R9098 T12350 T12351 nsubj PKC,is
R9099 T12351 T12351 ROOT is,is
R91 T123 T125 mark that,stimulated
R910 T1282 T1279 pobj NF-κB,by
R9100 T12352 T12351 acomp Essential,is
R9101 T12353 T12352 prep in,Essential
R9102 T12354 T12353 pcomp Regulating,in
R9103 T12355 T12356 compound NF-κB,Activity
R9104 T12356 T12359 compound Activity,activity
R9105 T12357 T12359 compound M-CSF-dependent,activity
R9106 T12358 T12359 compound PKC,activity
R9107 T12359 T12354 dobj activity,Regulating
R9108 T12360 T12351 ccomp facilitated,is
R9109 T12361 T12363 nsubj NF-κB,phosphorylation
R911 T1283 T1282 prep in,NF-κB
R9110 T12362 T12363 nummod p65,phosphorylation
R9111 T12363 T12360 dobj phosphorylation,facilitated
R9112 T12364 T12363 prep at,phosphorylation
R9113 T12365 T12364 pobj Ser276,at
R9114 T12366 T12364 cc but,at
R9115 T12367 T12366 neg not,but
R9116 T12368 T12363 appos Ser536,phosphorylation
R9117 T12369 T12351 punct .,is
R9118 T12370 T12371 aux To,confirm
R9119 T12371 T12385 advcl confirm,co-transfected
R912 T1284 T1285 compound murine,macrophages
R9120 T12372 T12373 det the,role
R9121 T12373 T12371 dobj role,confirm
R9122 T12374 T12373 prep of,role
R9123 T12375 T12374 pobj PKC,of
R9124 T12376 T12373 cc and,role
R9125 T12377 T12379 nummod p65,phosphorylation
R9126 T12378 T12379 compound Ser276,phosphorylation
R9127 T12379 T12373 conj phosphorylation,role
R9128 T12380 T12379 prep on,phosphorylation
R9129 T12381 T12382 amod NF-κB,activity
R913 T1285 T1283 pobj macrophages,in
R9130 T12382 T12380 pobj activity,on
R9131 T12383 T12385 punct ",",co-transfected
R9132 T12384 T12385 nsubj we,co-transfected
R9133 T12385 T12385 ROOT co-transfected,co-transfected
R9134 T12386 T12387 det the,NF-κB-SEAP
R9135 T12387 T12388 nsubj NF-κB-SEAP,construct
R9136 T12388 T12385 ccomp construct,co-transfected
R9137 T12389 T12388 prep with,construct
R9138 T12390 T12393 det either,p65
R9139 T12391 T12392 compound WT,NF-κB
R914 T1286 T1288 quantmod [,]
R9140 T12392 T12393 compound NF-κB,p65
R9141 T12393 T12389 pobj p65,with
R9142 T12394 T12393 cc or,p65
R9143 T12395 T12397 compound NF-κB,276S/A
R9144 T12396 T12397 compound p65,276S/A
R9145 T12397 T12398 nsubj 276S/A,constructs
R9146 T12398 T12393 conj constructs,p65
R9147 T12399 T12398 prep in,constructs
R9148 T12400 T12399 pobj Raw,in
R9149 T12401 T12402 nummod 264.7,cells
R915 T1287 T1288 nummod 11,]
R9150 T12402 T12398 conj cells,constructs
R9151 T12403 T12405 punct ",",treated
R9152 T12404 T12405 advmod then,treated
R9153 T12405 T12385 dep treated,co-transfected
R9154 T12406 T12405 dobj cells,treated
R9155 T12407 T12405 prep with,treated
R9156 T12408 T12411 det the,Ro-31-8220
R9157 T12409 T12411 compound PKC,Ro-31-8220
R9158 T12410 T12411 compound inhibitor,Ro-31-8220
R9159 T12411 T12407 pobj Ro-31-8220,with
R916 T1288 T1282 appos ],NF-κB
R9160 T12412 T12411 prep in,Ro-31-8220
R9161 T12413 T12414 det the,absence
R9162 T12414 T12412 pobj absence,in
R9163 T12415 T12411 cc or,Ro-31-8220
R9164 T12416 T12411 conj presences,Ro-31-8220
R9165 T12417 T12416 prep of,presences
R9166 T12418 T12417 pobj M-CSF,of
R9167 T12419 T12385 punct .,co-transfected
R9168 T12420 T12421 mark As,expected
R9169 T12421 T12424 advcl expected,transfected
R917 T1289 T1267 punct .,induces
R9170 T12422 T12421 prep in,expected
R9171 T12423 T12422 pobj cells,in
R9172 T12424 T12424 ROOT transfected,transfected
R9173 T12425 T12424 prep with,transfected
R9174 T12426 T12427 compound vector,control
R9175 T12427 T12425 pobj control,with
R9176 T12428 T12424 punct ",",transfected
R9177 T12429 T12424 advcl inhibiting,transfected
R9178 T12430 T12431 nsubj PKC,reduced
R9179 T12431 T12431 ROOT reduced,reduced
R918 T1290 T1296 prep In,is
R9180 T12432 T12434 amod M-CSF-stimulated,activity
R9181 T12433 T12434 compound NF-κB,activity
R9182 T12434 T12431 dobj activity,reduced
R9183 T12435 T12431 prep compared,reduced
R9184 T12436 T12435 prep to,compared
R9185 T12437 T12436 pobj cells,to
R9186 T12438 T12437 acl treated,cells
R9187 T12439 T12438 prep with,treated
R9188 T12440 T12439 pobj M-CSF,with
R9189 T12441 T12440 cc and,M-CSF
R919 T1291 T1290 pobj monocytes,In
R9190 T12442 T12443 det the,vehicle
R9191 T12443 T12440 conj vehicle,M-CSF
R9192 T12444 T12443 appos DMSO,vehicle
R9193 T12445 T12444 punct (,DMSO
R9194 T12446 T12444 appos p,DMSO
R9195 T12447 T12448 punct =,0.001
R9196 T12448 T12446 prep 0.001,p
R9197 T12449 T12444 punct ),DMSO
R9198 T12450 T12452 punct (,8A
R9199 T12451 T12452 compound Figure,8A
R92 T124 T125 nsubj M-CSF,stimulated
R920 T1292 T1291 cc and,monocytes
R9200 T12452 T12444 appos 8A,DMSO
R9201 T12453 T12452 punct ),8A
R9202 T12454 T12431 punct .,reduced
R9203 T12455 T12466 prep In,increased
R9204 T12456 T12455 pobj contrast,In
R9205 T12457 T12466 punct ",",increased
R9206 T12458 T12466 csubj expressing,increased
R9207 T12459 T12461 compound WT,p65
R9208 T12460 T12461 compound NF-κB,p65
R9209 T12461 T12458 dobj p65,expressing
R921 T1293 T1291 conj macrophages,monocytes
R9210 T12462 T12461 punct (,p65
R9211 T12463 T12464 nummod p65,WT
R9212 T12464 T12461 appos WT,p65
R9213 T12465 T12461 punct ),p65
R9214 T12466 T12466 ROOT increased,increased
R9215 T12467 T12468 amod NF-κB,activity
R9216 T12468 T12466 dobj activity,increased
R9217 T12469 T12466 punct ",",increased
R9218 T12470 T12476 mark while,decreased
R9219 T12471 T12474 det the,inhibitor
R922 T1294 T1296 punct ",",is
R9220 T12472 T12474 amod general,inhibitor
R9221 T12473 T12474 compound PKC,inhibitor
R9222 T12474 T12476 nsubj inhibitor,decreased
R9223 T12475 T12476 nsubj Ro-31-8220,decreased
R9224 T12476 T12466 advcl decreased,increased
R9225 T12477 T12479 det this,activity
R9226 T12478 T12479 amod NF-κB,activity
R9227 T12479 T12476 dobj activity,decreased
R9228 T12480 T12479 punct (,activity
R9229 T12481 T12479 appos p,activity
R923 T1295 T1296 nsubj NF-κB,is
R9230 T12482 T12483 punct =,0.001
R9231 T12483 T12481 pobj 0.001,p
R9232 T12484 T12479 punct ),activity
R9233 T12485 T12466 punct .,increased
R9234 T12486 T12497 advmod Notably,reduced
R9235 T12487 T12497 punct ",",reduced
R9236 T12488 T12489 det the,introduction
R9237 T12489 T12497 nsubj introduction,reduced
R9238 T12490 T12489 prep of,introduction
R9239 T12491 T12490 pobj NF-κB,of
R924 T1296 T1296 ROOT is,is
R9240 T12492 T12491 nummod p65,NF-κB
R9241 T12493 T12494 nummod 276,S/A
R9242 T12494 T12489 appos S/A,introduction
R9243 T12495 T12497 aux construct,reduced
R9244 T12496 T12497 advmod significantly,reduced
R9245 T12497 T12497 ROOT reduced,reduced
R9246 T12498 T12499 amod NF-κB,activity
R9247 T12499 T12497 dobj activity,reduced
R9248 T12500 T12497 prep compared,reduced
R9249 T12501 T12500 prep with,compared
R925 T1297 T1300 det an,factor
R9250 T12502 T12505 det the,p65
R9251 T12503 T12504 compound WT,NF-κB
R9252 T12504 T12505 compound NF-κB,p65
R9253 T12505 T12501 pobj p65,with
R9254 T12506 T12497 conj construct,reduced
R9255 T12507 T12506 punct (,construct
R9256 T12508 T12506 nmod p,construct
R9257 T12509 T12510 punct =,0.005
R9258 T12510 T12508 prep 0.005,p
R9259 T12511 T12508 punct ),p
R926 T1298 T1300 amod important,factor
R9260 T12512 T12508 cc and,p
R9261 T12513 T12514 compound M-CSF,treatment
R9262 T12514 T12515 nsubj treatment,was
R9263 T12515 T12508 conj was,p
R9264 T12516 T12515 acomp unable,was
R9265 T12517 T12518 aux to,overcome
R9266 T12518 T12516 xcomp overcome,unable
R9267 T12519 T12520 det this,inhibition
R9268 T12520 T12518 dobj inhibition,overcome
R9269 T12521 T12515 punct .,was
R927 T1299 T1300 amod transcriptional,factor
R9270 T12522 T12525 advmod Furthermore,co-transfected
R9271 T12523 T12525 punct ",",co-transfected
R9272 T12524 T12525 nsubj we,co-transfected
R9273 T12525 T12525 ROOT co-transfected,co-transfected
R9274 T12526 T12527 det the,NF-κB-SEAP
R9275 T12527 T12528 nsubj NF-κB-SEAP,construct
R9276 T12528 T12525 ccomp construct,co-transfected
R9277 T12529 T12528 prep along,construct
R9278 T12530 T12529 prep with,along
R9279 T12531 T12535 preconj either,p65
R928 T1300 T1296 attr factor,is
R9280 T12532 T12535 det the,p65
R9281 T12533 T12534 compound WT,NF-κB
R9282 T12534 T12535 compound NF-κB,p65
R9283 T12535 T12530 pobj p65,with
R9284 T12536 T12535 punct ",",p65
R9285 T12537 T12539 nmod NF-κB,276S/A
R9286 T12538 T12539 nummod p65,276S/A
R9287 T12539 T12535 conj 276S/A,p65
R9288 T12540 T12539 cc or,276S/A
R9289 T12541 T12539 conj NF-κB,276S/A
R929 T1301 T1300 prep for,factor
R9290 T12542 T12543 compound p65,536S/A
R9291 T12543 T12539 conj 536S/A,276S/A
R9292 T12544 T12535 conj constructs,p65
R9293 T12545 T12528 prep into,construct
R9294 T12546 T12555 det a,line
R9295 T12547 T12555 nmod NF-κB,line
R9296 T12548 T12549 nummod p65,−
R9297 T12549 T12555 nmod −,line
R9298 T12550 T12555 nmod /,line
R9299 T12551 T12555 nmod −,line
R93 T125 T122 ccomp stimulated,demonstrate
R930 T1302 T1301 pobj expression,for
R9300 T12552 T12555 nmod murine,line
R9301 T12553 T12555 nmod fibroblast,line
R9302 T12554 T12555 compound cell,line
R9303 T12555 T12545 pobj line,into
R9304 T12556 T12528 cc and,construct
R9305 T12557 T12559 amod measured,activity
R9306 T12558 T12559 amod NF-κB,activity
R9307 T12559 T12528 conj activity,construct
R9308 T12560 T12525 punct .,co-transfected
R9309 T12561 T12562 mark As,predicted
R931 T1303 T1302 prep of,expression
R9310 T12562 T12582 advcl predicted,activated
R9311 T12563 T12562 punct ",",predicted
R9312 T12564 T12582 advcl expression,activated
R9313 T12565 T12564 prep of,expression
R9314 T12566 T12567 compound WT,NF-κB
R9315 T12567 T12568 compound NF-κB,p65
R9316 T12568 T12565 pobj p65,of
R9317 T12569 T12568 punct (,p65
R9318 T12570 T12571 nummod p65,WT
R9319 T12571 T12568 appos WT,p65
R932 T1304 T1303 pobj cytokines,of
R9320 T12572 T12568 punct ),p65
R9321 T12573 T12564 prep in,expression
R9322 T12574 T12573 pobj NF-κB,in
R9323 T12575 T12576 nummod p65,−
R9324 T12576 T12577 nummod −,/
R9325 T12577 T12580 compound /,line
R9326 T12578 T12580 compound −,line
R9327 T12579 T12580 compound cell,line
R9328 T12580 T12582 nsubj line,activated
R9329 T12581 T12582 advmod constitutively,activated
R933 T1305 T1304 cc and,cytokines
R9330 T12582 T12582 ROOT activated,activated
R9331 T12583 T12582 dobj NF-κB,activated
R9332 T12584 T12582 prep compared,activated
R9333 T12585 T12584 prep to,compared
R9334 T12586 T12585 pobj cells,to
R9335 T12587 T12586 acl transfected,cells
R9336 T12588 T12587 prep with,transfected
R9337 T12589 T12590 compound vector,control
R9338 T12590 T12588 pobj control,with
R9339 T12591 T12587 prep without,transfected
R934 T1306 T1307 compound cell,surface
R9340 T12592 T12593 det any,stimulation
R9341 T12593 T12591 pobj stimulation,without
R9342 T12594 T12596 punct (,8B
R9343 T12595 T12596 compound Figure,8B
R9344 T12596 T12593 appos 8B,stimulation
R9345 T12597 T12596 punct ),8B
R9346 T12598 T12582 punct .,activated
R9347 T12599 T12607 prep In,construct
R9348 T12600 T12599 pobj comparison,In
R9349 T12601 T12607 punct ",",construct
R935 T1307 T1308 compound surface,receptors
R9350 T12602 T12607 nsubj transfecting,construct
R9351 T12603 T12604 det the,NF-κB
R9352 T12604 T12606 compound NF-κB,276S/A
R9353 T12605 T12606 compound p65,276S/A
R9354 T12606 T12607 nsubj 276S/A,construct
R9355 T12607 T12607 ROOT construct,construct
R9356 T12608 T12610 amod reduced,activity
R9357 T12609 T12610 amod NF-κB,activity
R9358 T12610 T12607 dobj activity,construct
R9359 T12611 T12607 prep by,construct
R936 T1308 T1304 conj receptors,cytokines
R9360 T12612 T12611 pobj 5-fold,by
R9361 T12613 T12624 punct (,276S/A
R9362 T12614 T12616 advmod p,0.006
R9363 T12615 T12616 punct =,0.006
R9364 T12616 T12624 nummod 0.006,276S/A
R9365 T12617 T12619 punct ",",NF-κB
R9366 T12618 T12619 compound WT,NF-κB
R9367 T12619 T12624 npadvmod NF-κB,276S/A
R9368 T12620 T12619 nummod p65,NF-κB
R9369 T12621 T12622 nmod vs.,NF-κB
R937 T1309 T1311 nmod [,]
R9370 T12622 T12624 nmod NF-κB,276S/A
R9371 T12623 T12624 nummod p65,276S/A
R9372 T12624 T12612 appos 276S/A,5-fold
R9373 T12625 T12624 punct ),276S/A
R9374 T12626 T12607 punct ",",construct
R9375 T12627 T12628 mark while,expressing
R9376 T12628 T12607 advcl expressing,construct
R9377 T12629 T12632 det the,536S/A
R9378 T12630 T12632 compound NF-κB,536S/A
R9379 T12631 T12632 compound p65,536S/A
R938 T1310 T1311 nummod 12,]
R9380 T12632 T12633 nsubj 536S/A,construct
R9381 T12633 T12628 ccomp construct,expressing
R9382 T12634 T12636 amod increased,activity
R9383 T12635 T12636 amod NF-κB,activity
R9384 T12636 T12633 dobj activity,construct
R9385 T12637 T12633 prep in,construct
R9386 T12638 T12639 det the,p65
R9387 T12639 T12637 pobj p65,in
R9388 T12640 T12633 conj −,construct
R9389 T12641 T12643 nummod /,cells
R939 T1311 T1300 appos ],factor
R9390 T12642 T12643 amod −,cells
R9391 T12643 T12640 dobj cells,−
R9392 T12644 T12640 prep to,−
R9393 T12645 T12644 pobj levels,to
R9394 T12646 T12645 amod similar,levels
R9395 T12647 T12646 prep to,similar
R9396 T12648 T12649 compound WT,NF-κB
R9397 T12649 T12647 pobj NF-κB,to
R9398 T12650 T12652 nummod p65,cells
R9399 T12651 T12652 amod transfected,cells
R94 T126 T128 compound NF-κB,activity
R940 T1312 T1296 punct .,is
R9400 T12652 T12645 conj cells,levels
R9401 T12653 T12607 punct .,construct
R9402 T12654 T12678 prep Since,examined
R9403 T12655 T12657 amod M-CSF-induced,activation
R9404 T12656 T12657 compound PKC,activation
R9405 T12657 T12658 nsubj activation,regulated
R9406 T12658 T12660 amod regulated,activity
R9407 T12659 T12660 amod NF-κB,activity
R9408 T12660 T12654 pobj activity,Since
R9409 T12661 T12660 prep via,activity
R941 T1313 T1320 advmod However,is
R9410 T12662 T12663 compound Ser276,residue
R9411 T12663 T12661 pobj residue,via
R9412 T12664 T12663 prep of,residue
R9413 T12665 T12664 pobj NF-κB,of
R9414 T12666 T12665 nummod p65,NF-κB
R9415 T12667 T12660 prep in,activity
R9416 T12668 T12670 amod primary,MDMs
R9417 T12669 T12670 amod human,MDMs
R9418 T12670 T12667 pobj MDMs,in
R9419 T12671 T12670 cc and,MDMs
R942 T1314 T1320 punct ",",is
R9420 T12672 T12670 conj RAW,MDMs
R9421 T12673 T12674 nummod 264.7,cells
R9422 T12674 T12660 appos cells,activity
R9423 T12675 T12678 punct ",",examined
R9424 T12676 T12678 nsubj we,examined
R9425 T12677 T12678 advmod next,examined
R9426 T12678 T12678 ROOT examined,examined
R9427 T12679 T12681 mark if,occurred
R9428 T12680 T12681 nsubj this,occurred
R9429 T12681 T12678 advcl occurred,examined
R943 T1315 T1320 prep unlike,is
R9430 T12682 T12681 prep in,occurred
R9431 T12683 T12682 pobj NF-κB,in
R9432 T12684 T12683 nummod p65,NF-κB
R9433 T12685 T12688 nummod −,fibroblasts
R9434 T12686 T12687 compound /,−
R9435 T12687 T12688 compound −,fibroblasts
R9436 T12688 T12681 npadvmod fibroblasts,occurred
R9437 T12689 T12681 prep in,occurred
R9438 T12690 T12689 pobj response,in
R9439 T12691 T12690 prep to,response
R944 T1316 T1317 amod resting,T-cells
R9440 T12692 T12694 det a,stimulus
R9441 T12693 T12694 amod native,stimulus
R9442 T12694 T12691 pobj stimulus,to
R9443 T12695 T12694 prep for,stimulus
R9444 T12696 T12697 det these,cells
R9445 T12697 T12695 pobj cells,for
R9446 T12698 T12694 punct ",",stimulus
R9447 T12699 T12694 appos TNFα,stimulus
R9448 T12700 T12678 punct .,examined
R9449 T12701 T12702 amod NF-κB,activity
R945 T1317 T1315 pobj T-cells,unlike
R9450 T12702 T12704 nsubjpass activity,measured
R9451 T12703 T12704 auxpass was,measured
R9452 T12704 T12704 ROOT measured,measured
R9453 T12705 T12704 prep in,measured
R9454 T12706 T12708 det the,NF-κB
R9455 T12707 T12708 compound WT,NF-κB
R9456 T12708 T12705 pobj NF-κB,in
R9457 T12709 T12708 nummod p65,NF-κB
R9458 T12710 T12708 cc or,NF-κB
R9459 T12711 T12708 conj NF-κB,NF-κB
R946 T1318 T1320 punct ",",is
R9460 T12712 T12713 compound p65,276S/A
R9461 T12713 T12708 conj 276S/A,NF-κB
R9462 T12714 T12715 amod transfected,cells
R9463 T12715 T12704 advcl cells,measured
R9464 T12716 T12715 acl treated,cells
R9465 T12717 T12716 prep with,treated
R9466 T12718 T12717 pobj TNFα,with
R9467 T12719 T12716 prep in,treated
R9468 T12720 T12721 det the,absence
R9469 T12721 T12719 pobj absence,in
R947 T1319 T1320 nsubj NF-κB,is
R9470 T12722 T12721 cc or,absence
R9471 T12723 T12721 conj presence,absence
R9472 T12724 T12721 prep of,absence
R9473 T12725 T12724 pobj Ro-31-8220,of
R9474 T12726 T12728 punct (,8C
R9475 T12727 T12728 compound Figure,8C
R9476 T12728 T12725 appos 8C,Ro-31-8220
R9477 T12729 T12725 punct ),Ro-31-8220
R9478 T12730 T12704 punct .,measured
R9479 T12731 T12732 mark As,expected
R948 T1320 T1320 ROOT is,is
R9480 T12732 T12735 advcl expected,increased
R9481 T12733 T12735 punct ",",increased
R9482 T12734 T12735 nsubj TNFα,increased
R9483 T12735 T12735 ROOT increased,increased
R9484 T12736 T12737 amod NF-κB,activity
R9485 T12737 T12735 dobj activity,increased
R9486 T12738 T12735 prep in,increased
R9487 T12739 T12738 pobj NF-κB,in
R9488 T12740 T12741 nummod p65,−
R9489 T12741 T12744 nummod −,cells
R949 T1321 T1322 advmod constitutively,present
R9490 T12742 T12743 compound /,−
R9491 T12743 T12744 compound −,cells
R9492 T12744 T12735 conj cells,increased
R9493 T12745 T12744 acl expressing,cells
R9494 T12746 T12745 dobj WT,expressing
R9495 T12747 T12746 amod p65,WT
R9496 T12748 T12744 cc and,cells
R9497 T12749 T12744 punct (,cells
R9498 T12750 T12744 conj p,cells
R9499 T12751 T12752 punct =,0.001
R95 T127 T128 compound transcriptional,activity
R950 T1322 T1320 acomp present,is
R9500 T12752 T12750 pobj 0.001,p
R9501 T12753 T12744 punct ),cells
R9502 T12754 T12755 compound PKC,inhibitors
R9503 T12755 T12756 nsubj inhibitors,decreased
R9504 T12756 T12756 ROOT decreased,decreased
R9505 T12757 T12758 amod NF-κB,activity
R9506 T12758 T12756 dobj activity,decreased
R9507 T12759 T12756 prep to,decreased
R9508 T12760 T12761 det the,non-stimulated
R9509 T12761 T12759 pobj non-stimulated,to
R951 T1323 T1322 prep in,present
R9510 T12762 T12765 punct (,level
R9511 T12763 T12765 nmod NS,level
R9512 T12764 T12765 punct ),level
R9513 T12765 T12761 appos level,non-stimulated
R9514 T12766 T12765 punct (,level
R9515 T12767 T12765 npadvmod p,level
R9516 T12768 T12769 punct =,0.007
R9517 T12769 T12767 prep 0.007,p
R9518 T12770 T12765 punct ),level
R9519 T12771 T12756 punct .,decreased
R952 T1324 T1325 det the,nuclei
R9520 T12772 T12779 advcl Introducing,reduced
R9521 T12773 T12774 amod NF-κB,p65
R9522 T12774 T12772 dobj p65,Introducing
R9523 T12775 T12777 nummod 276,constructs
R9524 T12776 T12777 compound S/A,constructs
R9525 T12777 T12779 nsubj constructs,reduced
R9526 T12778 T12779 advmod significantly,reduced
R9527 T12779 T12779 ROOT reduced,reduced
R9528 T12780 T12781 amod NF-κB,activity
R9529 T12781 T12779 dobj activity,reduced
R953 T1325 T1323 pobj nuclei,in
R9530 T12782 T12779 prep compared,reduced
R9531 T12783 T12782 prep with,compared
R9532 T12784 T12787 det the,p65
R9533 T12785 T12786 compound WT,NF-κB
R9534 T12786 T12787 compound NF-κB,p65
R9535 T12787 T12783 pobj p65,with
R9536 T12788 T12779 conj construct,reduced
R9537 T12789 T12788 punct (,construct
R9538 T12790 T12788 dobj p,construct
R9539 T12791 T12792 punct =,0.001
R954 T1326 T1325 prep of,nuclei
R9540 T12792 T12790 prep 0.001,p
R9541 T12793 T12790 punct ),p
R9542 T12794 T12779 punct .,reduced
R9543 T12795 T12798 advmod Notably,failed
R9544 T12796 T12798 punct ",",failed
R9545 T12797 T12798 nsubj TNFα,failed
R9546 T12798 T12798 ROOT failed,failed
R9547 T12799 T12800 aux to,increase
R9548 T12800 T12798 xcomp increase,failed
R9549 T12801 T12802 amod NF-κB,activity
R955 T1327 T1328 amod primary,monocytes
R9550 T12802 T12800 dobj activity,increase
R9551 T12803 T12800 prep in,increase
R9552 T12804 T12810 det the,cells
R9553 T12805 T12810 nmod NF-κB,cells
R9554 T12806 T12807 nummod p65,−
R9555 T12807 T12810 nummod −,cells
R9556 T12808 T12809 compound /,−
R9557 T12809 T12810 compound −,cells
R9558 T12810 T12803 pobj cells,in
R9559 T12811 T12810 acl expressing,cells
R956 T1328 T1326 pobj monocytes,of
R9560 T12812 T12814 compound NF-κB,276S/A
R9561 T12813 T12814 compound p65,276S/A
R9562 T12814 T12815 compound 276S/A,constructs
R9563 T12815 T12811 dobj constructs,expressing
R9564 T12816 T12798 punct .,failed
R9565 T12817 T12818 det These,observations
R9566 T12818 T12819 nsubj observations,are
R9567 T12819 T12819 ROOT are,are
R9568 T12820 T12819 acomp similar,are
R9569 T12821 T12820 prep to,similar
R957 T1329 T1328 cc and,monocytes
R9570 T12822 T12821 pobj macrophages,to
R9571 T12823 T12822 acl overexpressing,macrophages
R9572 T12824 T12827 det the,276S/A
R9573 T12825 T12827 nmod NF-κB,276S/A
R9574 T12826 T12827 nummod p65,276S/A
R9575 T12827 T12823 dobj 276S/A,overexpressing
R9576 T12828 T12827 punct (,276S/A
R9577 T12829 T12830 compound Figure,8A
R9578 T12830 T12827 appos 8A,276S/A
R9579 T12831 T12827 punct ),276S/A
R958 T1330 T1332 amod monocytic,lines
R9580 T12832 T12819 punct .,are
R9581 T12833 T12834 poss Our,data
R9582 T12834 T12835 nsubj data,demonstrate
R9583 T12835 T12835 ROOT demonstrate,demonstrate
R9584 T12836 T12841 mark that,is
R9585 T12837 T12841 nsubj Ser276,is
R9586 T12838 T12837 prep of,Ser276
R9587 T12839 T12838 pobj NF-κB,of
R9588 T12840 T12839 nummod p65,NF-κB
R9589 T12841 T12835 ccomp is,demonstrate
R959 T1331 T1332 compound cell,lines
R9590 T12842 T12841 acomp essential,is
R9591 T12843 T12841 prep in,is
R9592 T12844 T12843 pcomp regulating,in
R9593 T12845 T12846 amod NF-κB,activity
R9594 T12846 T12844 dobj activity,regulating
R9595 T12847 T12841 cc and,is
R9596 T12848 T12841 conj suggests,is
R9597 T12849 T12851 mark that,regulates
R9598 T12850 T12851 nsubj PKC,regulates
R9599 T12851 T12848 ccomp regulates,suggests
R96 T128 T125 dobj activity,stimulated
R960 T1332 T1328 conj lines,monocytes
R9600 T12852 T12853 amod NF-κB,activity
R9601 T12853 T12851 dobj activity,regulates
R9602 T12854 T12851 prep by,regulates
R9603 T12855 T12854 pcomp modulating,by
R9604 T12856 T12857 det the,phosphorylation
R9605 T12857 T12855 dobj phosphorylation,modulating
R9606 T12858 T12857 prep of,phosphorylation
R9607 T12859 T12860 compound NF-κB,p65
R9608 T12860 T12858 pobj p65,of
R9609 T12861 T12855 prep at,modulating
R961 T1333 T1322 prep in,present
R9610 T12862 T12863 compound Ser276,residue
R9611 T12863 T12861 pobj residue,at
R9612 T12864 T12835 punct .,demonstrate
R962 T1334 T1335 det the,absence
R963 T1335 T1333 pobj absence,in
R964 T1336 T1335 prep of,absence
R965 T1337 T1338 amod exogenous,stimuli
R966 T1338 T1336 pobj stimuli,of
R967 T1339 T1340 mark as,demonstrated
R968 T1340 T1322 advcl demonstrated,present
R969 T1341 T1340 agent by,demonstrated
R97 T129 T128 prep in,activity
R970 T1342 T1343 compound mobility,shift
R971 T1343 T1344 compound shift,analysis
R972 T1344 T1345 compound analysis,[
R973 T1345 T1347 nmod [,]
R974 T1346 T1347 nummod 13,]
R975 T1347 T1341 pobj ],by
R976 T1348 T1320 punct .,is
R977 T1349 T1355 advmod Similarly,observed
R978 T1350 T1355 punct ",",observed
R979 T1351 T1352 advmod constitutively,active
R98 T130 T132 amod human,macrophages
R980 T1352 T1353 amod active,NF-κB
R981 T1353 T1355 nsubjpass NF-κB,observed
R982 T1354 T1355 auxpass was,observed
R983 T1355 T1355 ROOT observed,observed
R984 T1356 T1355 prep in,observed
R985 T1357 T1359 amod human,macrophages
R986 T1358 T1359 amod alveolar,macrophages
R987 T1359 T1356 pobj macrophages,in
R988 T1360 T1362 nmod [,]
R989 T1361 T1362 nummod 14,]
R99 T131 T132 amod monocyte-derived,macrophages
R990 T1362 T1355 dobj ],observed
R991 T1363 T1355 punct .,observed
R992 T1364 T1374 prep In,sequestered
R993 T1365 T1367 det the,pathway
R994 T1366 T1367 amod classic/canonical,pathway
R995 T1367 T1364 pobj pathway,In
R996 T1368 T1374 punct ",",sequestered
R997 T1369 T1374 det the,sequestered
R998 T1370 T1374 nsubjpass NF-κB,sequestered
R999 T1371 T1372 nummod p50/p65,complex