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PMC:3148254 JSONTXT 20 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T135 0-7 NNP denotes HOIL-1L
T136 8-19 NNP denotes Interacting
T137 20-27 NNP denotes Protein
T138 28-29 -LRB- denotes (
T139 29-33 NNP denotes HOIP
T140 33-34 -RRB- denotes )
T141 35-37 VBZ denotes Is
T142 38-47 JJ denotes Essential
T143 48-51 IN denotes for
T144 52-56 NNP denotes CD40
T145 57-66 NNP denotes Signaling
T146 115-119 NNP denotes CD40
T147 120-122 VBZ denotes is
T148 123-124 DT denotes a
T149 125-129 NN denotes cell
T150 130-137 NN denotes surface
T151 138-146 NN denotes receptor
T152 147-156 JJ denotes important
T153 157-159 IN denotes in
T154 160-163 DT denotes the
T155 164-174 NN denotes activation
T156 175-177 IN denotes of
T157 178-196 JJ denotes antigen-presenting
T158 197-202 NNS denotes cells
T159 203-209 IN denotes during
T160 210-216 JJ denotes immune
T161 217-226 NNS denotes responses
T162 226-227 . denotes .
T163 228-230 IN denotes In
T164 231-242 NNS denotes macrophages
T165 243-246 CC denotes and
T166 247-256 JJ denotes dendritic
T167 257-262 NNS denotes cells
T168 262-263 , denotes ,
T169 264-274 NN denotes engagement
T170 275-277 IN denotes of
T171 278-282 CD denotes CD40
T172 283-285 IN denotes by
T173 286-289 PRP$ denotes its
T174 290-296 NN denotes ligand
T175 297-302 NNP denotes CD154
T176 303-311 VBZ denotes provides
T177 312-319 NNS denotes signals
T178 320-328 JJ denotes critical
T179 329-332 IN denotes for
T180 333-347 JJ denotes anti-microbial
T181 348-351 CC denotes and
T182 352-353 NNP denotes T
T183 354-367 JJ denotes cell-mediated
T184 368-374 JJ denotes immune
T185 375-384 NNS denotes responses
T186 384-385 , denotes ,
T187 386-398 RB denotes respectively
T188 398-399 . denotes .
T189 400-402 IN denotes In
T190 403-404 NNP denotes B
T191 405-410 NNS denotes cells
T192 410-411 , denotes ,
T193 412-416 NNP denotes CD40
T194 417-426 VBG denotes signaling
T195 427-430 VBZ denotes has
T196 431-432 DT denotes a
T197 433-438 JJ denotes major
T198 439-443 NN denotes role
T199 444-446 IN denotes in
T200 447-457 VBG denotes regulating
T201 458-462 NN denotes cell
T202 463-476 NN denotes proliferation
T203 476-477 , denotes ,
T204 478-486 NN denotes antibody
T205 487-497 NN denotes production
T206 497-498 , denotes ,
T207 499-502 CC denotes and
T208 503-509 NN denotes memory
T209 510-511 NNP denotes B
T210 512-516 NN denotes cell
T211 517-528 NN denotes development
T212 528-529 . denotes .
T213 530-534 CD denotes CD40
T214 535-545 NN denotes engagement
T215 546-553 NNS denotes results
T216 554-556 IN denotes in
T217 557-560 DT denotes the
T218 561-570 NN denotes formation
T219 571-573 IN denotes of
T220 574-575 DT denotes a
T221 576-595 JJ denotes receptor-associated
T222 596-603 NN denotes complex
T223 604-608 WDT denotes that
T224 609-617 VBZ denotes mediates
T225 618-628 NN denotes activation
T226 629-631 IN denotes of
T227 632-637 NNP denotes NF-κB
T228 637-638 , denotes ,
T229 639-655 JJ denotes stress-activated
T230 656-663 NN denotes protein
T231 664-671 NNS denotes kinases
T232 671-672 , denotes ,
T233 673-676 CC denotes and
T234 677-682 JJ denotes other
T235 683-692 VBG denotes signaling
T236 693-702 NNS denotes molecules
T237 702-703 . denotes .
T238 704-711 RB denotes However
T239 711-712 , denotes ,
T240 713-716 DT denotes the
T241 717-727 NNS denotes mechanisms
T242 728-732 WDT denotes that
T243 733-737 VBP denotes link
T244 738-742 CD denotes CD40
T245 743-745 TO denotes to
T246 746-751 DT denotes these
T247 752-761 VBG denotes signaling
T248 762-768 NNS denotes events
T249 769-773 VBP denotes have
T250 774-778 VBN denotes been
T251 779-783 RB denotes only
T252 784-793 RB denotes partially
T253 794-807 VBN denotes characterized
T254 807-808 . denotes .
T255 809-814 VBN denotes Known
T256 815-825 NNS denotes components
T257 826-828 IN denotes of
T258 829-832 DT denotes the
T259 833-837 NNP denotes CD40
T260 838-847 VBG denotes signaling
T261 848-855 JJ denotes complex
T262 856-863 VBP denotes include
T263 864-871 NNS denotes members
T264 872-874 IN denotes of
T265 875-878 DT denotes the
T266 879-882 NNP denotes TNF
T267 883-902 JJ denotes receptor-associated
T268 903-909 NN denotes factor
T269 910-911 -LRB- denotes (
T270 911-915 NNP denotes TRAF
T271 915-916 -RRB- denotes )
T272 917-923 NN denotes family
T273 924-926 IN denotes of
T274 927-935 NNS denotes proteins
T275 935-936 . denotes .
T276 937-939 PRP denotes We
T277 940-950 RB denotes previously
T278 951-957 VBD denotes showed
T279 958-962 IN denotes that
T280 963-966 DT denotes the
T281 967-971 NNP denotes TRAF
T282 972-978 NN denotes family
T283 979-985 NN denotes member
T284 986-991 CD denotes TRAF2
T285 992-1000 VBZ denotes mediates
T286 1001-1012 NN denotes recruitment
T287 1013-1015 IN denotes of
T288 1016-1035 JJ denotes HOIL-1L-interacting
T289 1036-1043 NN denotes protein
T290 1044-1045 -LRB- denotes (
T291 1045-1049 NNP denotes HOIP
T292 1049-1050 -RRB- denotes )
T293 1051-1053 TO denotes to
T294 1054-1057 DT denotes the
T295 1058-1069 JJ denotes cytoplasmic
T296 1070-1076 NN denotes domain
T297 1077-1079 IN denotes of
T298 1080-1084 NNP denotes CD40
T299 1084-1085 , denotes ,
T300 1086-1096 VBG denotes suggesting
T301 1097-1101 IN denotes that
T302 1102-1106 NNP denotes HOIP
T303 1107-1110 VBZ denotes has
T304 1111-1112 DT denotes a
T305 1113-1117 NN denotes role
T306 1118-1120 IN denotes in
T307 1121-1124 DT denotes the
T308 1125-1129 NNP denotes CD40
T309 1130-1139 VBG denotes signaling
T310 1140-1147 NN denotes pathway
T311 1147-1148 . denotes .
T312 1149-1151 TO denotes To
T313 1152-1161 VB denotes determine
T314 1162-1165 DT denotes the
T315 1166-1170 NN denotes role
T316 1171-1173 IN denotes of
T317 1174-1178 NNP denotes HOIP
T318 1179-1181 IN denotes in
T319 1182-1186 NNP denotes CD40
T320 1187-1196 VBG denotes signaling
T321 1196-1197 , denotes ,
T322 1198-1200 PRP denotes we
T323 1201-1205 VBD denotes used
T324 1206-1213 JJ denotes somatic
T325 1214-1218 NN denotes cell
T326 1219-1223 NN denotes gene
T327 1224-1233 VBG denotes targeting
T328 1234-1236 TO denotes to
T329 1237-1245 VB denotes generate
T330 1246-1251 NN denotes mouse
T331 1252-1253 NNP denotes B
T332 1254-1258 NN denotes cell
T333 1259-1264 NNS denotes lines
T334 1265-1274 VBP denotes deficient
T335 1275-1277 IN denotes in
T336 1278-1282 NNP denotes HOIP
T337 1282-1283 . denotes .
T338 1284-1286 PRP denotes We
T339 1287-1292 VBD denotes found
T340 1293-1297 IN denotes that
T341 1298-1301 DT denotes the
T342 1302-1314 JJ denotes CD40-induced
T343 1315-1327 NN denotes upregulation
T344 1328-1330 IN denotes of
T345 1331-1335 NNP denotes CD80
T346 1336-1339 CC denotes and
T347 1340-1350 NN denotes activation
T348 1351-1353 IN denotes of
T349 1354-1362 NN denotes germline
T350 1363-1377 NN denotes immunoglobulin
T351 1378-1385 NN denotes epsilon
T352 1386-1399 NN denotes transcription
T353 1400-1404 VBD denotes were
T354 1405-1414 JJ denotes defective
T355 1415-1417 IN denotes in
T356 1418-1432 JJ denotes HOIP-deficient
T357 1433-1438 NNS denotes cells
T358 1438-1439 . denotes .
T359 1440-1442 PRP denotes We
T360 1443-1447 RB denotes also
T361 1448-1453 VBD denotes found
T362 1454-1458 IN denotes that
T363 1459-1462 DT denotes the
T364 1463-1476 JJ denotes CD40-mediated
T365 1477-1487 NN denotes activation
T366 1488-1490 IN denotes of
T367 1491-1496 NN denotes NF-κB
T368 1497-1500 CC denotes and
T369 1501-1506 JJ denotes c-Jun
T370 1507-1513 NN denotes kinase
T371 1514-1517 VBD denotes was
T372 1518-1526 VBN denotes impaired
T373 1526-1527 . denotes .
T374 1528-1539 NN denotes Recruitment
T375 1540-1542 IN denotes of
T376 1543-1546 NNP denotes IκB
T377 1547-1553 NN denotes kinase
T378 1554-1562 NNS denotes proteins
T379 1563-1565 TO denotes to
T380 1566-1569 DT denotes the
T381 1570-1574 NNP denotes CD40
T382 1575-1584 VBG denotes signaling
T383 1585-1592 NN denotes complex
T384 1593-1596 VBD denotes was
T385 1597-1609 JJ denotes undetectable
T386 1610-1612 IN denotes in
T387 1613-1627 JJ denotes HOIP-deficient
T388 1628-1633 NNS denotes cells
T389 1633-1634 , denotes ,
T390 1635-1646 RB denotes potentially
T391 1647-1657 VBG denotes explaining
T392 1658-1661 DT denotes the
T393 1662-1668 NN denotes defect
T394 1669-1671 IN denotes in
T395 1672-1677 JJ denotes NF-κB
T396 1678-1688 NN denotes activation
T397 1688-1689 . denotes .
T398 1690-1701 NNP denotes Restoration
T399 1702-1704 IN denotes of
T400 1705-1709 NNP denotes HOIP
T401 1710-1720 NN denotes expression
T402 1721-1729 VBD denotes reversed
T403 1730-1733 DT denotes the
T404 1734-1741 NNS denotes defects
T405 1742-1744 IN denotes in
T406 1745-1753 JJ denotes cellular
T407 1754-1764 NN denotes activation
T408 1765-1768 CC denotes and
T409 1769-1778 VBG denotes signaling
T410 1778-1779 . denotes .
T411 1780-1785 DT denotes These
T412 1786-1793 NNS denotes results
T413 1794-1800 VBP denotes reveal
T414 1801-1805 NNP denotes HOIP
T415 1806-1808 IN denotes as
T416 1809-1810 DT denotes a
T417 1811-1814 JJ denotes key
T418 1815-1824 NN denotes component
T419 1825-1827 IN denotes of
T420 1828-1831 DT denotes the
T421 1832-1836 NNP denotes CD40
T422 1837-1846 VBG denotes signaling
T423 1847-1854 NN denotes pathway
T424 1854-1855 . denotes .
T1199 1870-1874 CD denotes CD40
T1200 1875-1884 VBG denotes signaling
T1201 1885-1887 IN denotes in
T1202 1888-1900 JJ denotes professional
T1203 1901-1919 JJ denotes antigen-presenting
T1204 1920-1925 NNS denotes cells
T1205 1925-1926 , denotes ,
T1206 1927-1936 VBG denotes including
T1207 1937-1938 NNP denotes B
T1208 1939-1944 NNS denotes cells
T1209 1944-1945 , denotes ,
T1210 1946-1957 NNS denotes macrophages
T1211 1957-1958 , denotes ,
T1212 1959-1962 CC denotes and
T1213 1963-1972 JJ denotes dendritic
T1214 1973-1978 NNS denotes cells
T1215 1978-1979 , denotes ,
T1216 1980-1982 VBZ denotes is
T1217 1983-1991 JJ denotes critical
T1218 1992-1995 IN denotes for
T1219 1996-1999 DT denotes the
T1220 2000-2009 JJ denotes efficient
T1221 2010-2020 NN denotes activation
T1222 2021-2023 IN denotes of
T1223 2024-2031 JJ denotes humoral
T1224 2032-2035 CC denotes and
T1225 2036-2049 JJ denotes cell-mediated
T1226 2050-2056 JJ denotes immune
T1227 2057-2066 NNS denotes responses
T1228 2067-2068 VBP denotes [
T1229 2068-2069 CD denotes 1
T1230 2069-2070 NNP denotes ]
T1231 2070-2071 , denotes ,
T1232 2072-2073 NNP denotes [
T1233 2073-2074 CD denotes 2
T1234 2074-2075 NNP denotes ]
T1235 2075-2076 , denotes ,
T1236 2077-2078 NNP denotes [
T1237 2078-2079 CD denotes 3
T1238 2079-2080 NNP denotes ]
T1239 2080-2081 . denotes .
T1240 2082-2086 CD denotes CD40
T1241 2087-2096 VBG denotes signaling
T1242 2097-2099 VBZ denotes is
T1243 2100-2109 VBN denotes activated
T1244 2110-2112 IN denotes in
T1245 2113-2114 DT denotes a
T1246 2115-2116 NNP denotes T
T1247 2117-2131 NN denotes cell-dependent
T1248 2132-2138 NN denotes manner
T1249 2138-2139 , denotes ,
T1250 2140-2142 IN denotes as
T1251 2143-2146 DT denotes the
T1252 2147-2153 NN denotes ligand
T1253 2154-2157 IN denotes for
T1254 2158-2162 NNP denotes CD40
T1255 2162-2163 , denotes ,
T1256 2164-2169 NNP denotes CD154
T1257 2169-2170 , denotes ,
T1258 2171-2173 VBZ denotes is
T1259 2174-2183 VBN denotes expressed
T1260 2184-2193 RB denotes primarily
T1261 2194-2196 IN denotes by
T1262 2197-2206 VBN denotes activated
T1263 2207-2208 NNP denotes T
T1264 2209-2214 NNS denotes cells
T1265 2214-2215 . denotes .
T1266 2216-2220 CD denotes CD40
T1267 2221-2231 NN denotes engagement
T1268 2232-2237 VBZ denotes leads
T1269 2238-2240 TO denotes to
T1270 2241-2244 DT denotes the
T1271 2245-2255 NN denotes activation
T1272 2256-2258 IN denotes of
T1273 2259-2266 JJ denotes various
T1274 2267-2276 VBG denotes signaling
T1275 2277-2286 NNS denotes molecules
T1276 2286-2287 , denotes ,
T1277 2288-2297 VBG denotes including
T1278 2298-2314 JJ denotes stress-activated
T1279 2315-2322 NN denotes protein
T1280 2323-2330 NNS denotes kinases
T1281 2331-2334 CC denotes and
T1282 2335-2338 DT denotes the
T1283 2339-2352 NN denotes transcription
T1284 2353-2359 NN denotes factor
T1285 2360-2365 NNP denotes NF-κB
T1286 2365-2366 , denotes ,
T1287 2367-2372 WDT denotes which
T1288 2373-2383 VBP denotes upregulate
T1289 2384-2387 DT denotes the
T1290 2388-2398 NN denotes expression
T1291 2399-2401 IN denotes of
T1292 2402-2411 NNS denotes cytokines
T1293 2412-2415 CC denotes and
T1294 2416-2421 JJ denotes other
T1295 2422-2429 NNS denotes factors
T1296 2430-2434 WDT denotes that
T1297 2435-2442 VBP denotes promote
T1298 2443-2449 JJ denotes immune
T1299 2450-2459 NNS denotes responses
T1300 2459-2460 . denotes .
T1301 2461-2464 DT denotes The
T1302 2465-2474 NN denotes mechanism
T1303 2475-2477 IN denotes by
T1304 2478-2483 WDT denotes which
T1305 2484-2488 NNP denotes CD40
T1306 2489-2496 VBZ denotes induces
T1307 2497-2502 DT denotes these
T1308 2503-2512 VBG denotes signaling
T1309 2513-2521 NNS denotes pathways
T1310 2522-2525 VBZ denotes has
T1311 2526-2529 RB denotes not
T1312 2530-2534 VBN denotes been
T1313 2535-2545 RB denotes completely
T1314 2546-2553 VBN denotes defined
T1315 2553-2554 . denotes .
T1316 2555-2558 DT denotes The
T1317 2559-2570 JJ denotes cytoplasmic
T1318 2571-2577 NN denotes domain
T1319 2578-2580 IN denotes of
T1320 2581-2585 NNP denotes CD40
T1321 2586-2590 VBZ denotes does
T1322 2591-2594 RB denotes not
T1323 2595-2601 VB denotes appear
T1324 2602-2604 TO denotes to
T1325 2605-2609 VB denotes have
T1326 2610-2619 JJ denotes intrinsic
T1327 2620-2629 JJ denotes enzymatic
T1328 2630-2638 NN denotes activity
T1329 2638-2639 , denotes ,
T1330 2640-2643 CC denotes but
T1331 2644-2646 VBZ denotes is
T1332 2647-2651 JJ denotes able
T1333 2652-2654 TO denotes to
T1334 2655-2662 VB denotes mediate
T1335 2663-2672 VBG denotes signaling
T1336 2673-2680 IN denotes through
T1337 2681-2684 DT denotes the
T1338 2685-2696 NN denotes recruitment
T1339 2697-2699 IN denotes of
T1340 2700-2707 JJ denotes several
T1341 2708-2721 JJ denotes intracellular
T1342 2722-2730 NNS denotes proteins
T1343 2730-2731 . denotes .
T1344 2732-2739 NNS denotes Members
T1345 2740-2742 IN denotes of
T1346 2743-2746 DT denotes the
T1347 2747-2750 NNP denotes TNF
T1348 2751-2770 JJ denotes receptor-associated
T1349 2771-2777 NN denotes factor
T1350 2778-2779 -LRB- denotes (
T1351 2779-2783 NNP denotes TRAF
T1352 2783-2784 -RRB- denotes )
T1353 2785-2791 NN denotes family
T1354 2791-2792 , denotes ,
T1355 2793-2802 VBG denotes including
T1356 2803-2808 CD denotes TRAF1
T1357 2808-2809 , denotes ,
T1358 2810-2815 CD denotes TRAF2
T1359 2815-2816 , denotes ,
T1360 2817-2822 CD denotes TRAF3
T1361 2822-2823 , denotes ,
T1362 2824-2827 CC denotes and
T1363 2828-2833 NNP denotes TRAF6
T1364 2833-2834 , denotes ,
T1365 2835-2841 VBP denotes appear
T1366 2842-2844 TO denotes to
T1367 2845-2847 VB denotes be
T1368 2848-2860 RB denotes particularly
T1369 2861-2870 JJ denotes important
T1370 2871-2874 IN denotes for
T1371 2875-2878 DT denotes the
T1372 2879-2889 NN denotes initiation
T1373 2890-2893 CC denotes and
T1374 2894-2904 NN denotes regulation
T1375 2905-2907 IN denotes of
T1376 2908-2912 NNP denotes CD40
T1377 2913-2922 VBG denotes signaling
T1378 2923-2924 NNP denotes [
T1379 2924-2925 CD denotes 4
T1380 2925-2926 NNP denotes ]
T1381 2926-2927 . denotes .
T1382 2928-2933 DT denotes These
T1383 2934-2942 NNS denotes proteins
T1384 2943-2951 VBP denotes function
T1385 2952-2954 IN denotes in
T1386 2955-2959 NN denotes part
T1387 2960-2962 IN denotes as
T1388 2963-2970 NN denotes adaptor
T1389 2971-2980 NNS denotes molecules
T1390 2980-2981 , denotes ,
T1391 2982-2989 JJ denotes binding
T1392 2990-2992 TO denotes to
T1393 2993-2996 DT denotes the
T1394 2997-3008 JJ denotes cytoplasmic
T1395 3009-3013 NN denotes tail
T1396 3014-3016 IN denotes of
T1397 3017-3021 NNP denotes CD40
T1398 3022-3025 CC denotes and
T1399 3026-3036 VBG denotes recruiting
T1400 3037-3042 JJ denotes other
T1401 3043-3051 NNS denotes proteins
T1402 3052-3054 TO denotes to
T1403 3055-3058 DT denotes the
T1404 3059-3078 JJ denotes receptor-associated
T1405 3079-3086 NN denotes complex
T1406 3086-3087 . denotes .
T1407 3088-3092 DT denotes Some
T1408 3093-3095 IN denotes of
T1409 3096-3099 DT denotes the
T1410 3100-3105 NNS denotes TRAFs
T1411 3106-3110 RB denotes also
T1412 3111-3119 VBP denotes function
T1413 3120-3122 IN denotes as
T1414 3123-3125 CD denotes E3
T1415 3126-3135 NN denotes ubiquitin
T1416 3136-3143 NNS denotes ligases
T1417 3143-3144 , denotes ,
T1418 3145-3148 CC denotes and
T1419 3149-3153 DT denotes this
T1420 3154-3163 JJ denotes enzymatic
T1421 3164-3172 NN denotes activity
T1422 3173-3176 MD denotes may
T1423 3177-3187 VB denotes contribute
T1424 3188-3190 TO denotes to
T1425 3191-3197 VB denotes signal
T1426 3198-3209 NN denotes propagation
T1427 3210-3213 CC denotes and
T1428 3214-3224 NN denotes regulation
T1429 3224-3225 . denotes .
T1430 3226-3231 IN denotes Among
T1431 3232-3235 DT denotes the
T1432 3236-3244 JJ denotes multiple
T1433 3245-3250 NNS denotes TRAFs
T1434 3251-3255 WDT denotes that
T1435 3256-3265 VBP denotes associate
T1436 3266-3270 IN denotes with
T1437 3271-3275 NNP denotes CD40
T1438 3275-3276 , denotes ,
T1439 3277-3282 NNP denotes TRAF3
T1440 3283-3286 MD denotes can
T1441 3287-3295 VB denotes function
T1442 3296-3298 IN denotes as
T1443 3299-3300 DT denotes a
T1444 3301-3309 JJ denotes negative
T1445 3310-3319 NN denotes regulator
T1446 3320-3322 IN denotes of
T1447 3323-3332 VBG denotes signaling
T1448 3332-3333 , denotes ,
T1449 3334-3339 IN denotes while
T1450 3340-3345 CD denotes TRAF2
T1451 3346-3349 CC denotes and
T1452 3350-3355 CD denotes TRAF6
T1453 3356-3363 VB denotes promote
T1454 3364-3367 DT denotes the
T1455 3368-3378 NN denotes activation
T1456 3379-3381 IN denotes of
T1457 3382-3392 JJ denotes downstream
T1458 3393-3402 VBG denotes signaling
T1459 3403-3411 NNS denotes pathways
T1460 3412-3413 NNP denotes [
T1461 3413-3414 CD denotes 4
T1462 3414-3415 NNP denotes ]
T1463 3415-3416 . denotes .
T1464 3417-3419 PRP denotes We
T1465 3420-3428 RB denotes recently
T1466 3429-3441 VBD denotes demonstrated
T1467 3442-3446 IN denotes that
T1468 3447-3454 JJ denotes HOIL-1L
T1469 3455-3466 VBG denotes interacting
T1470 3467-3474 NN denotes protein
T1471 3475-3476 -LRB- denotes (
T1472 3476-3480 NNP denotes HOIP
T1473 3480-3481 -RRB- denotes )
T1474 3481-3482 , denotes ,
T1475 3483-3484 DT denotes a
T1476 3485-3494 NN denotes ubiquitin
T1477 3495-3501 NN denotes ligase
T1478 3502-3506 WDT denotes that
T1479 3507-3510 MD denotes can
T1480 3511-3519 VB denotes catalyze
T1481 3520-3523 DT denotes the
T1482 3524-3532 NN denotes assembly
T1483 3533-3535 IN denotes of
T1484 3536-3542 JJ denotes linear
T1485 3543-3556 NN denotes polyubiquitin
T1486 3557-3563 NNS denotes chains
T1487 3564-3565 VBP denotes [
T1488 3565-3566 CD denotes 5
T1489 3566-3567 NNP denotes ]
T1490 3567-3568 , denotes ,
T1491 3569-3571 VBZ denotes is
T1492 3572-3581 VBN denotes recruited
T1493 3582-3584 TO denotes to
T1494 3585-3589 NNP denotes CD40
T1495 3590-3592 IN denotes in
T1496 3593-3594 DT denotes a
T1497 3595-3610 JJ denotes TRAF2-dependent
T1498 3611-3617 NN denotes manner
T1499 3618-3627 VBG denotes following
T1500 3628-3638 NN denotes engagement
T1501 3639-3641 IN denotes of
T1502 3642-3646 CD denotes CD40
T1503 3647-3649 IN denotes by
T1504 3650-3659 JJ denotes agonistic
T1505 3660-3668 NN denotes antibody
T1506 3669-3670 NN denotes [
T1507 3670-3671 CD denotes 6
T1508 3671-3672 NNP denotes ]
T1509 3672-3673 . denotes .
T1510 3674-3679 DT denotes These
T1511 3680-3683 CC denotes and
T1512 3684-3689 JJ denotes other
T1513 3690-3698 NNS denotes findings
T1514 3699-3702 VBD denotes led
T1515 3703-3705 PRP denotes us
T1516 3706-3708 TO denotes to
T1517 3709-3720 VB denotes hypothesize
T1518 3721-3725 DT denotes that
T1519 3726-3730 NNP denotes HOIP
T1520 3731-3740 NNS denotes functions
T1521 3741-3751 JJ denotes downstream
T1522 3752-3754 IN denotes of
T1523 3755-3760 CD denotes TRAF2
T1524 3761-3763 IN denotes in
T1525 3764-3767 DT denotes the
T1526 3768-3772 NNP denotes CD40
T1527 3773-3782 VBG denotes signaling
T1528 3783-3790 NN denotes pathway
T1529 3791-3794 CC denotes and
T1530 3795-3799 IN denotes that
T1531 3800-3804 NNP denotes HOIP
T6730 3800-3804 NNP denotes HOIP
T1532 3805-3807 VBZ denotes is
T6731 3805-3807 VBZ denotes is
T1533 3808-3817 JJ denotes necessary
T6732 3808-3817 JJ denotes necessary
T1534 3818-3821 IN denotes for
T6733 3818-3821 IN denotes for
T1535 3822-3825 DT denotes the
T1536 3826-3836 NN denotes activation
T1537 3837-3839 IN denotes of
T1538 3840-3845 NNP denotes NF-κB
T1539 3846-3849 CC denotes and
T1540 3850-3858 RB denotes possibly
T1541 3859-3864 JJ denotes other
T1542 3865-3874 VBG denotes signaling
T1543 3875-3884 NNS denotes molecules
T1544 3884-3885 . denotes .
T1545 3886-3888 TO denotes To
T1546 3889-3893 VB denotes test
T1547 3894-3898 DT denotes this
T1548 3899-3909 NN denotes hypothesis
T1549 3909-3910 , denotes ,
T1550 3911-3913 PRP denotes we
T1551 3914-3922 VBD denotes employed
T1552 3923-3930 JJ denotes somatic
T1553 3931-3935 NN denotes cell
T1554 3936-3940 NN denotes gene
T1555 3941-3950 VBG denotes targeting
T1556 3951-3953 TO denotes to
T1557 3954-3960 VB denotes ablate
T1558 3961-3971 NN denotes expression
T1559 3972-3974 IN denotes of
T1560 3975-3979 NNP denotes HOIP
T1561 3980-3982 IN denotes in
T1562 3983-3984 DT denotes a
T1563 3985-3990 NN denotes mouse
T1564 3991-3992 NNP denotes B
T1565 3993-3997 NN denotes cell
T1566 3998-4002 NN denotes line
T1567 4003-4007 WDT denotes that
T1568 4008-4011 VBZ denotes has
T1569 4012-4018 VBN denotes proven
T1570 4019-4021 TO denotes to
T1571 4022-4024 VB denotes be
T1572 4025-4026 DT denotes a
T1573 4027-4033 JJ denotes useful
T1574 4034-4039 NN denotes model
T1575 4040-4043 IN denotes for
T1576 4044-4045 NNP denotes B
T1577 4046-4050 NN denotes cell
T1578 4051-4055 CD denotes CD40
T1579 4056-4065 VBG denotes signaling
T1580 4066-4067 NN denotes [
T1581 4067-4068 CD denotes 7
T1582 4068-4069 NNP denotes ]
T1583 4069-4070 , denotes ,
T1584 4071-4072 NNP denotes [
T1585 4072-4073 CD denotes 8
T1586 4073-4074 NNP denotes ]
T1587 4074-4075 , denotes ,
T1588 4076-4077 NNP denotes [
T1589 4077-4078 CD denotes 9
T1590 4078-4079 NNP denotes ]
T1591 4079-4080 . denotes .
T1592 4081-4083 PRP denotes We
T1593 4084-4089 VBD denotes found
T1594 4090-4094 IN denotes that
T1595 4095-4098 DT denotes the
T1596 4099-4111 JJ denotes CD40-induced
T1597 4112-4124 NN denotes upregulation
T1598 4125-4127 IN denotes of
T1599 4128-4132 NNP denotes CD80
T1600 4133-4134 -LRB- denotes (
T1601 4134-4135 DT denotes a
T1602 4136-4149 JJ denotes costimulatory
T1603 4150-4158 NN denotes molecule
T1604 4159-4162 IN denotes for
T1605 4163-4164 NNP denotes T
T1606 4165-4170 NNS denotes cells
T1607 4170-4171 -RRB- denotes )
T1608 4172-4175 VBD denotes was
T1609 4176-4185 JJ denotes defective
T1610 4186-4188 IN denotes in
T1611 4189-4203 JJ denotes HOIP-deficient
T1612 4204-4209 NNS denotes cells
T1613 4209-4210 . denotes .
T1614 4211-4220 RB denotes Similarly
T1615 4220-4221 , denotes ,
T1616 4222-4225 DT denotes the
T1617 4226-4230 NNP denotes CD40
T1618 4231-4234 CC denotes and
T1619 4235-4239 NN denotes IL-4
T1620 4240-4246 VBN denotes driven
T1621 4247-4257 NN denotes production
T1622 4258-4260 IN denotes of
T1623 4261-4269 NN denotes germline
T1624 4270-4281 NNS denotes transcripts
T1625 4282-4286 IN denotes from
T1626 4287-4290 DT denotes the
T1627 4291-4305 NN denotes immunoglobulin
T1628 4306-4313 NN denotes epsilon
T1629 4314-4319 JJ denotes heavy
T1630 4320-4325 NN denotes chain
T1631 4326-4331 NN denotes locus
T1632 4331-4332 , denotes ,
T1633 4333-4335 DT denotes an
T1634 4336-4341 NN denotes event
T1635 4342-4346 WDT denotes that
T1636 4347-4355 VBZ denotes precedes
T1637 4356-4370 NN denotes immunoglobulin
T1638 4371-4375 NN denotes gene
T1639 4376-4389 NN denotes rearrangement
T1640 4390-4393 CC denotes and
T1641 4394-4401 NN denotes isotype
T1642 4402-4411 NN denotes switching
T1643 4411-4412 , denotes ,
T1644 4413-4416 VBD denotes was
T1645 4417-4426 JJ denotes defective
T1646 4427-4429 IN denotes in
T1647 4430-4433 DT denotes the
T1648 4434-4441 NN denotes absence
T1649 4442-4444 IN denotes of
T1650 4445-4449 NNP denotes HOIP
T1651 4449-4450 . denotes .
T1652 4451-4453 PRP denotes We
T1653 4454-4458 RB denotes also
T1654 4459-4464 VBD denotes found
T1655 4465-4469 IN denotes that
T1656 4470-4473 DT denotes the
T1657 4474-4487 JJ denotes CD40-mediated
T1658 4488-4498 NN denotes activation
T1659 4499-4501 IN denotes of
T1660 4502-4507 NNP denotes NF-κB
T1661 4508-4511 CC denotes and
T1662 4512-4515 DT denotes the
T1663 4516-4532 JJ denotes stress-activated
T1664 4533-4540 NN denotes protein
T1665 4541-4547 NN denotes kinase
T1666 4548-4553 JJ denotes c-Jun
T1667 4554-4560 NN denotes kinase
T1668 4561-4562 -LRB- denotes (
T1669 4562-4565 NNP denotes JNK
T1670 4565-4566 -RRB- denotes )
T1671 4567-4570 VBD denotes was
T1672 4571-4580 JJ denotes defective
T1673 4581-4583 IN denotes in
T1674 4584-4598 JJ denotes HOIP-deficient
T1675 4599-4604 NNS denotes cells
T1676 4604-4605 . denotes .
T1677 4606-4616 JJ denotes Consistent
T1678 4617-4621 IN denotes with
T1679 4622-4630 JJ denotes impaired
T1680 4631-4636 NN denotes NF-κB
T1681 4637-4647 NN denotes activation
T1682 4647-4648 , denotes ,
T1683 4649-4660 NN denotes association
T6734 4649-4660 NN denotes association
T1684 4661-4663 IN denotes of
T6735 4661-4663 IN denotes of
T1685 4664-4667 DT denotes the
T6736 4664-4667 DT denotes the
T1686 4668-4671 NNP denotes IκB
T6737 4668-13422 NNP denotes IκB kinase (IKK) complex with CD40 was undetectable in HOIP-deficient cells. Together, our results indicate that HOIP plays a critical role in the activation of signaling pathways that regulate cellular responses to CD40 engagement. Results Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6]. Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations). We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation. These results support the hypothesis that HOIP plays a role in CD40 signal transduction. To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J. This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9]. We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A). Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele. To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase. This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted. Two independent clonal cell lines were chosen for further analysis. HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful. In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells. 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting. (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown. Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination. Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence. A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place. LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence. The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones. A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+). HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown. Approximate molecular weight of HOIP is 120 kD. To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B). These cells are referred to as HOIP-reconstituted cells. In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector). HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC. To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2). Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested. In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31. 10.1371/journal.pone.0023061.g002 Figure 2 CD40-mediated CD80 upregulation is defective in HOIP-deficient cells. Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody). Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not. Similar results were obtained in a second experiment. CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12]. This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response. Switching to IgE can be induced by the combination of IL-4 and CD40 signals. The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13]. To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3). Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected. In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4. Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4. Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling. 10.1371/journal.pone.0023061.g003 Figure 3 GLε transcription is defective in CD40-stimulated HOIP-deficient cells. A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4. RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts. Results were normalized to the levels of Hprt1 transcripts in each sample. Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values). Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone. HOIP mediates CD40-stimulated NF-κB and JNK activation The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells. It follows that HOIP is likely a key mediator of CD40 signaling. To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4]. Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody. Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK). In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm. Phosphorylation by the IKK
T1687 4672-4678 NN denotes kinase
T1688 4679-4680 -LRB- denotes (
T1689 4680-4683 NNP denotes IKK
T1690 4683-4684 -RRB- denotes )
T1691 4685-4692 JJ denotes complex
T1692 4693-4697 IN denotes with
T1693 4698-4702 NNP denotes CD40
T1694 4703-4706 VBD denotes was
T1695 4707-4719 JJ denotes undetectable
T1696 4720-4722 IN denotes in
T1697 4723-4737 JJ denotes HOIP-deficient
T1698 4738-4743 NNS denotes cells
T1699 4743-4744 . denotes .
T1700 4745-4753 RB denotes Together
T1701 4753-4754 , denotes ,
T1702 4755-4758 PRP$ denotes our
T1703 4759-4766 NNS denotes results
T1704 4767-4775 VBP denotes indicate
T1705 4776-4780 IN denotes that
T1706 4781-4785 NNP denotes HOIP
T1707 4786-4791 VBZ denotes plays
T1708 4792-4793 DT denotes a
T1709 4794-4802 JJ denotes critical
T1710 4803-4807 NN denotes role
T1711 4808-4810 IN denotes in
T1712 4811-4814 DT denotes the
T1713 4815-4825 NN denotes activation
T1714 4826-4828 IN denotes of
T1715 4829-4838 VBG denotes signaling
T1716 4839-4847 NNS denotes pathways
T1717 4848-4852 WDT denotes that
T1718 4853-4861 VBP denotes regulate
T1719 4862-4870 JJ denotes cellular
T1720 4871-4880 NNS denotes responses
T1721 4881-4883 TO denotes to
T1722 4884-4888 CD denotes CD40
T1723 4889-4899 NN denotes engagement
T1724 4899-4900 . denotes .
T2880 4911-4921 NNP denotes Generation
T2881 4922-4924 IN denotes of
T2882 4925-4939 NNP denotes HOIP-deficient
T2883 4940-4941 NNP denotes B
T2884 4942-4947 NNS denotes cells
T2885 4948-4951 IN denotes via
T2886 4952-4960 JJ denotes targeted
T2887 4961-4971 NN denotes disruption
T2888 4972-4974 IN denotes of
T2889 4975-4980 NNP denotes Rnf31
T2890 4980-4981 , denotes ,
T2891 4982-4985 DT denotes the
T2892 4986-4990 NN denotes gene
T2893 4991-4999 VBG denotes encoding
T2894 5000-5004 NNP denotes HOIP
T2895 5005-5007 IN denotes In
T2896 5008-5011 PRP$ denotes our
T2897 5012-5020 JJ denotes previous
T2898 5021-5026 NN denotes study
T2899 5026-5027 , denotes ,
T2900 5028-5030 PRP denotes we
T2901 5031-5043 VBD denotes demonstrated
T2902 5044-5048 IN denotes that
T2903 5049-5053 NNP denotes HOIP
T2904 5054-5056 VBZ denotes is
T2905 5057-5066 VBN denotes recruited
T2906 5067-5069 TO denotes to
T2907 5070-5073 DT denotes the
T2908 5074-5078 NNP denotes CD40
T2909 5079-5088 VBG denotes signaling
T2910 5089-5096 JJ denotes complex
T2911 5097-5099 IN denotes in
T2912 5100-5103 CD denotes two
T2913 5104-5109 NN denotes mouse
T2914 5110-5111 NNP denotes B
T2915 5112-5116 NN denotes cell
T2916 5117-5122 NNS denotes lines
T2917 5123-5124 VBP denotes [
T2918 5124-5125 CD denotes 6
T2919 5125-5126 NNP denotes ]
T2920 5126-5127 . denotes .
T2921 5128-5135 JJ denotes Similar
T2922 5136-5143 NNS denotes results
T2923 5144-5148 VBD denotes were
T2924 5149-5157 VBN denotes obtained
T2925 5158-5162 IN denotes with
T2926 5163-5168 NN denotes mouse
T2927 5169-5180 NNS denotes splenocytes
T2928 5180-5181 , denotes ,
T2929 5182-5192 VBG denotes indicating
T2930 5193-5197 IN denotes that
T2931 5198-5201 DT denotes the
T2932 5202-5213 NN denotes interaction
T2933 5214-5216 IN denotes of
T2934 5217-5221 CD denotes CD40
T2935 5222-5226 IN denotes with
T2936 5227-5231 NNP denotes HOIP
T2937 5232-5234 VBZ denotes is
T2938 5235-5238 RB denotes not
T2939 5239-5246 VBN denotes limited
T2940 5247-5249 TO denotes to
T2941 5250-5261 VBN denotes transformed
T2942 5262-5263 NNP denotes B
T2943 5264-5268 NN denotes cell
T2944 5269-5274 NNS denotes lines
T2945 5275-5276 -LRB- denotes (
T2946 5276-5287 JJ denotes unpublished
T2947 5288-5300 NNS denotes observations
T2948 5300-5301 -RRB- denotes )
T2949 5301-5302 . denotes .
T2950 5303-5305 PRP denotes We
T2951 5306-5310 RB denotes also
T2952 5311-5316 VBD denotes found
T2953 5317-5321 IN denotes that
T2954 5322-5325 DT denotes the
T2955 5326-5337 NN denotes recruitment
T2956 5338-5340 IN denotes of
T2957 5341-5345 NNP denotes HOIP
T2958 5346-5348 TO denotes to
T2959 5349-5353 NNP denotes CD40
T2960 5354-5357 VBD denotes was
T2961 5358-5373 JJ denotes TRAF2-dependent
T2962 5374-5377 CC denotes and
T2963 5378-5382 IN denotes that
T2964 5383-5397 NN denotes overexpression
T2965 5398-5400 IN denotes of
T2966 5401-5402 DT denotes a
T2967 5403-5412 VBN denotes truncated
T2968 5413-5417 NNP denotes HOIP
T2969 5418-5424 JJ denotes mutant
T2970 5425-5434 RB denotes partially
T2971 5435-5444 VBN denotes inhibited
T2972 5445-5458 JJ denotes CD40-mediated
T2973 5459-5464 NN denotes NF-κB
T2974 5465-5475 NN denotes activation
T2975 5475-5476 . denotes .
T2976 5477-5482 DT denotes These
T2977 5483-5490 NNS denotes results
T2978 5491-5498 VBP denotes support
T2979 5499-5502 DT denotes the
T2980 5503-5513 NN denotes hypothesis
T2981 5514-5518 WDT denotes that
T2982 5519-5523 NNP denotes HOIP
T2983 5524-5529 VBZ denotes plays
T2984 5530-5531 DT denotes a
T2985 5532-5536 NN denotes role
T2986 5537-5539 IN denotes in
T2987 5540-5544 CD denotes CD40
T2988 5545-5551 NN denotes signal
T2989 5552-5564 NN denotes transduction
T2990 5564-5565 . denotes .
T2991 5566-5568 TO denotes To
T2992 5569-5576 RBR denotes further
T2993 5577-5583 VB denotes define
T2994 5584-5587 DT denotes the
T2995 5588-5592 NN denotes role
T2996 5593-5596 CC denotes and
T2997 5597-5605 VB denotes evaluate
T2998 5606-5609 DT denotes the
T2999 5610-5620 NN denotes importance
T3000 5621-5623 IN denotes of
T3001 5624-5628 NNP denotes HOIP
T3002 5629-5631 IN denotes in
T3003 5632-5636 NNP denotes CD40
T3004 5637-5646 VBG denotes signaling
T3005 5646-5647 , denotes ,
T3006 5648-5650 PRP denotes we
T3007 5651-5655 VBD denotes used
T3008 5656-5663 JJ denotes somatic
T3009 5664-5668 NN denotes cell
T3010 5669-5673 NN denotes gene
T3011 5674-5683 VBG denotes targeting
T3012 5684-5686 TO denotes to
T3013 5687-5694 VB denotes disrupt
T3014 5695-5698 DT denotes the
T3015 5699-5703 NN denotes gene
T3016 5704-5712 VBG denotes encoding
T3017 5713-5717 NNP denotes HOIP
T3018 5718-5720 IN denotes in
T3019 5721-5724 DT denotes the
T3020 5725-5730 NN denotes mouse
T3021 5731-5732 NNP denotes B
T3022 5733-5737 NN denotes cell
T3023 5738-5742 NN denotes line
T3024 5743-5746 NNP denotes A20
T3025 5746-5748 CD denotes .2
T3026 5748-5749 NNP denotes J
T3027 5749-5750 . denotes .
T3028 5751-5755 DT denotes This
T3029 5756-5760 NN denotes cell
T3030 5761-5765 NN denotes line
T3031 5766-5769 VBZ denotes has
T3032 5770-5774 VBN denotes been
T3033 5775-5787 RB denotes particularly
T3034 5788-5794 JJ denotes useful
T3035 5795-5797 IN denotes in
T3036 5798-5801 DT denotes the
T3037 5802-5818 NN denotes characterization
T3038 5819-5821 IN denotes of
T3039 5822-5826 NNP denotes CD40
T3040 5827-5836 VBG denotes signaling
T3041 5837-5847 NNS denotes mechanisms
T3042 5848-5851 JJ denotes due
T3043 5852-5854 TO denotes to
T3044 5855-5858 DT denotes the
T3045 5859-5867 JJ denotes relative
T3046 5868-5872 NN denotes ease
T3047 5873-5877 IN denotes with
T3048 5878-5883 WDT denotes which
T3049 5884-5887 PRP$ denotes its
T3050 5888-5894 NN denotes genome
T3051 5895-5898 MD denotes can
T3052 5899-5901 VB denotes be
T3053 5902-5910 VBN denotes modified
T3054 5911-5913 IN denotes by
T3055 5914-5924 RB denotes homologous
T3056 5925-5938 NN denotes recombination
T3057 5939-5940 NN denotes [
T3058 5940-5941 CD denotes 7
T3059 5941-5942 NNP denotes ]
T3060 5942-5943 , denotes ,
T3061 5944-5945 NNP denotes [
T3062 5945-5946 CD denotes 8
T3063 5946-5947 NNP denotes ]
T3064 5947-5948 , denotes ,
T3065 5949-5950 NNP denotes [
T3066 5950-5951 CD denotes 9
T3067 5951-5952 NNP denotes ]
T3068 5952-5953 . denotes .
T3069 5954-5956 PRP denotes We
T3070 5957-5961 VBD denotes used
T3071 5962-5963 DT denotes a
T3072 5964-5973 VBG denotes targeting
T3073 5974-5980 NN denotes vector
T3074 5981-5988 JJ denotes capable
T3075 5989-5991 IN denotes of
T3076 5992-6002 VBG denotes undergoing
T3077 6003-6013 RB denotes homologous
T3078 6014-6027 NN denotes recombination
T3079 6028-6032 IN denotes with
T3080 6033-6038 NNP denotes Rnf31
T3081 6039-6040 -LRB- denotes (
T3082 6040-6043 DT denotes the
T3083 6044-6048 NN denotes gene
T3084 6049-6057 VBG denotes encoding
T3085 6058-6062 NNP denotes HOIP
T3086 6062-6063 -RRB- denotes )
T3087 6064-6066 TO denotes to
T3088 6067-6074 VB denotes disrupt
T3089 6075-6078 DT denotes the
T3090 6079-6085 NN denotes coding
T3091 6086-6094 NN denotes sequence
T3092 6095-6097 IN denotes of
T3093 6098-6101 DT denotes the
T3094 6102-6106 NN denotes gene
T3095 6107-6109 IN denotes in
T3096 6110-6114 NN denotes exon
T3097 6115-6116 CD denotes 5
T3098 6117-6118 -LRB- denotes (
T3099 6118-6122 NNP denotes Fig.
T3100 6123-6125 NNP denotes 1A
T3101 6125-6126 -RRB- denotes )
T3102 6126-6127 . denotes .
T3103 6128-6137 VBG denotes Following
T3104 6138-6150 NN denotes introduction
T3105 6151-6153 IN denotes of
T3106 6154-6157 DT denotes the
T3107 6158-6164 NN denotes vector
T3108 6164-6165 , denotes ,
T3109 6166-6169 DT denotes the
T3110 6170-6188 JJ denotes neomycin-resistant
T3111 6189-6195 NNS denotes clones
T3112 6196-6200 WDT denotes that
T3113 6201-6206 VBD denotes arose
T3114 6207-6211 VBD denotes were
T3115 6212-6220 VBN denotes screened
T3116 6221-6223 IN denotes by
T3117 6224-6227 NNP denotes PCR
T3118 6228-6241 NN denotes amplification
T3119 6242-6244 IN denotes of
T3120 6245-6252 JJ denotes genomic
T3121 6253-6256 NN denotes DNA
T3122 6257-6259 TO denotes to
T3123 6260-6268 VB denotes identify
T3124 6269-6274 NNS denotes cells
T3125 6275-6285 VBG denotes containing
T3126 6286-6287 DT denotes a
T3127 6288-6297 VBN denotes disrupted
T3128 6298-6303 NNP denotes Rnf31
T3129 6304-6310 NN denotes allele
T3130 6310-6311 . denotes .
T3131 6312-6314 TO denotes To
T3132 6315-6321 VB denotes remove
T3133 6322-6325 DT denotes the
T3134 6326-6336 JJ denotes selectable
T3135 6337-6343 NN denotes marker
T3136 6344-6348 NN denotes gene
T3137 6349-6357 NN denotes cassette
T3138 6358-6362 IN denotes from
T3139 6363-6366 DT denotes the
T3140 6367-6376 VBN denotes disrupted
T3141 6377-6382 NNP denotes Rnf31
T3142 6383-6389 NN denotes allele
T3143 6389-6390 , denotes ,
T3144 6391-6402 JJ denotes recombinant
T3145 6403-6407 NN denotes cell
T3146 6408-6413 NNS denotes lines
T3147 6414-6418 VBD denotes were
T3148 6419-6430 RB denotes transiently
T3149 6431-6442 VBN denotes transfected
T3150 6443-6447 IN denotes with
T3151 6448-6449 DT denotes a
T3152 6450-6457 NN denotes plasmid
T3153 6458-6462 WDT denotes that
T3154 6463-6470 VBZ denotes encodes
T3155 6471-6474 NNP denotes Cre
T3156 6475-6486 NN denotes recombinase
T3157 6486-6487 . denotes .
T3158 6488-6492 DT denotes This
T3159 6493-6497 NN denotes step
T3160 6498-6505 VBD denotes allowed
T3161 6506-6508 PRP denotes us
T3162 6509-6511 TO denotes to
T3163 6512-6519 VB denotes perform
T3164 6520-6521 DT denotes a
T3165 6522-6528 JJ denotes second
T3166 6529-6534 NN denotes round
T3167 6535-6537 IN denotes of
T3168 6538-6547 VBG denotes targeting
T3169 6548-6551 CC denotes and
T3170 6552-6556 NN denotes drug
T3171 6557-6566 NN denotes selection
T3172 6566-6567 , denotes ,
T3173 6568-6578 VBG denotes generating
T3174 6579-6584 NNS denotes cells
T3175 6585-6587 IN denotes in
T3176 6588-6593 WDT denotes which
T3177 6594-6598 DT denotes both
T3178 6599-6605 NNS denotes copies
T3179 6606-6608 IN denotes of
T3180 6609-6614 NNS denotes Rnf31
T3181 6615-6619 VBD denotes were
T3182 6620-6629 VBN denotes disrupted
T3183 6629-6630 . denotes .
T3184 6631-6634 CD denotes Two
T3185 6635-6646 JJ denotes independent
T3186 6647-6653 JJ denotes clonal
T3187 6654-6658 NN denotes cell
T3188 6659-6664 NNS denotes lines
T3189 6665-6669 VBD denotes were
T3190 6670-6676 VBN denotes chosen
T3191 6677-6680 IN denotes for
T3192 6681-6688 JJ denotes further
T3193 6689-6697 NN denotes analysis
T3194 6697-6698 . denotes .
T3195 6699-6703 NNP denotes HOIP
T3196 6704-6711 NN denotes protein
T3197 6712-6722 NN denotes expression
T3198 6723-6726 VBD denotes was
T3199 6727-6739 JJ denotes undetectable
T3200 6740-6742 IN denotes in
T3201 6743-6747 DT denotes both
T3202 6748-6752 NN denotes cell
T3203 6753-6758 NNS denotes lines
T3204 6758-6759 , denotes ,
T3205 6760-6762 IN denotes as
T3206 6763-6773 VBN denotes determined
T3207 6774-6776 IN denotes by
T3208 6777-6784 JJ denotes Western
T3209 6785-6789 NN denotes blot
T3210 6790-6798 NN denotes analysis
T3211 6799-6801 IN denotes of
T3212 6802-6806 NN denotes cell
T3213 6807-6814 NNS denotes lysates
T3214 6815-6816 -LRB- denotes (
T3215 6816-6820 NNP denotes Fig.
T3216 6821-6823 NNP denotes 1B
T3217 6823-6824 -RRB- denotes )
T3218 6824-6825 , denotes ,
T3219 6826-6839 VBG denotes demonstrating
T3220 6840-6844 IN denotes that
T3221 6845-6848 DT denotes the
T3222 6849-6858 VBG denotes targeting
T3223 6859-6866 NN denotes process
T3224 6867-6870 VBD denotes was
T3225 6871-6881 JJ denotes successful
T3226 6881-6882 . denotes .
T3227 6883-6885 IN denotes In
T3228 6886-6889 DT denotes the
T3229 6890-6894 NN denotes text
T3230 6895-6899 WDT denotes that
T3231 6900-6907 VBZ denotes follows
T3232 6907-6908 , denotes ,
T3233 6909-6912 DT denotes the
T3234 6913-6916 CD denotes two
T3235 6917-6930 JJ denotes gene-targeted
T3236 6931-6935 NN denotes cell
T3237 6936-6941 NNS denotes lines
T3238 6942-6945 VBP denotes are
T3239 6946-6954 VBN denotes referred
T3240 6955-6957 TO denotes to
T3241 6958-6960 IN denotes as
T3242 6961-6975 JJ denotes HOIP-deficient
T3243 6976-6981 NNS denotes cells
T3244 6981-6982 . denotes .
T14807 7027-7031 NNP denotes HOIP
T14808 7032-7036 NN denotes gene
T14809 7037-7046 VBG denotes targeting
T14810 7046-7047 . denotes .
T14811 7048-7049 -LRB- denotes (
T14812 7049-7050 NNP denotes A
T14813 7050-7051 -RRB- denotes )
T14814 7052-7059 NNS denotes Regions
T14815 7060-7062 IN denotes of
T14816 7063-7068 JJ denotes Rnf31
T14817 7069-7073 NN denotes gene
T14818 7074-7082 NN denotes sequence
T14819 7083-7084 -LRB- denotes (
T14820 7084-7090 NN denotes bottom
T14821 7090-7091 -RRB- denotes )
T14822 7092-7096 VBN denotes used
T14823 7097-7099 IN denotes in
T14824 7100-7103 DT denotes the
T14825 7104-7108 NNP denotes HOIP
T14826 7109-7113 NN denotes gene
T14827 7114-7123 VBG denotes targeting
T14828 7124-7130 NN denotes vector
T14829 7131-7132 -LRB- denotes (
T14830 7132-7135 JJ denotes top
T14831 7135-7136 -RRB- denotes )
T14832 7137-7140 VBP denotes are
T14833 7141-7146 VBN denotes shown
T14834 7146-7147 . denotes .
T14835 7148-7154 NNS denotes Arrows
T14836 7155-7156 -LRB- denotes (
T14837 7156-7157 NN denotes F
T14838 7158-7161 CC denotes and
T14839 7162-7163 NN denotes R
T14840 7163-7164 -RRB- denotes )
T14841 7165-7173 VBP denotes indicate
T14842 7174-7185 JJ denotes approximate
T14843 7186-7195 NNS denotes positions
T14844 7196-7198 IN denotes of
T14845 7199-7208 NNS denotes sequences
T14846 7209-7219 RB denotes homologous
T14847 7220-7222 TO denotes to
T14848 7223-7239 NNS denotes oligonucleotides
T14849 7240-7244 VBN denotes used
T14850 7245-7248 IN denotes for
T14851 7249-7261 JJ denotes PCR-mediated
T14852 7262-7271 NN denotes detection
T14853 7272-7274 IN denotes of
T14854 7275-7285 RB denotes homologous
T14855 7286-7299 NN denotes recombination
T14856 7299-7300 . denotes .
T14857 7301-7311 JJ denotes Homologous
T14858 7312-7325 NN denotes recombination
T14859 7326-7328 IN denotes of
T14860 7329-7332 DT denotes the
T14861 7333-7339 NN denotes vector
T14862 7340-7344 IN denotes with
T14863 7345-7350 JJ denotes Rnf31
T14864 7351-7359 VBD denotes resulted
T14865 7360-7362 IN denotes in
T14866 7363-7364 DT denotes a
T14867 7365-7370 JJ denotes small
T14868 7371-7382 JJ denotes chromosomal
T14869 7383-7391 NN denotes deletion
T14870 7392-7395 CC denotes and
T14871 7396-7399 DT denotes the
T14872 7400-7408 JJ denotes in-frame
T14873 7409-7418 NN denotes insertion
T14874 7419-7421 IN denotes of
T14875 7422-7430 NN denotes neomycin
T14876 7431-7449 NN denotes phosphotransferase
T14877 7450-7451 -LRB- denotes (
T14878 7451-7455 NNP denotes NeoR
T14879 7455-7456 -RRB- denotes )
T14880 7457-7461 IN denotes into
T14881 7462-7465 DT denotes the
T14882 7466-7480 JJ denotes amino-terminal
T14883 7481-7485 NNP denotes HOIP
T14884 7486-7492 NN denotes coding
T14885 7493-7501 NN denotes sequence
T14886 7501-7502 . denotes .
T14887 7503-7504 DT denotes A
T14888 7505-7515 NN denotes diphtheria
T14889 7516-7521 NN denotes toxin
T14890 7522-7523 -LRB- denotes (
T14891 7523-7525 NNP denotes DT
T14892 7525-7526 -RRB- denotes )
T14893 7527-7535 NN denotes cassette
T14894 7536-7538 IN denotes in
T14895 7539-7542 DT denotes the
T14896 7543-7549 NN denotes vector
T14897 7550-7561 VBZ denotes facilitates
T14898 7562-7565 DT denotes the
T14899 7566-7574 JJ denotes negative
T14900 7575-7584 NN denotes selection
T14901 7585-7587 IN denotes of
T14902 7588-7593 NNS denotes cells
T14903 7594-7596 IN denotes in
T14904 7597-7602 WDT denotes which
T14905 7603-7609 JJ denotes random
T14906 7610-7621 JJ denotes chromosomal
T14907 7622-7631 NN denotes insertion
T14908 7632-7634 IN denotes of
T14909 7635-7638 DT denotes the
T14910 7639-7645 NN denotes vector
T14911 7646-7651 VBZ denotes takes
T14912 7652-7657 NN denotes place
T14913 7657-7658 . denotes .
T14914 7659-7663 NNP denotes LoxP
T14915 7664-7673 NNS denotes sequences
T14916 7674-7679 VBP denotes allow
T14917 7680-7683 DT denotes the
T14918 7684-7696 JJ denotes Cre-mediated
T14919 7697-7705 NN denotes deletion
T14920 7706-7708 IN denotes of
T14921 7709-7712 DT denotes the
T14922 7713-7717 NNP denotes NeoR
T14923 7718-7724 NN denotes coding
T14924 7725-7733 NN denotes sequence
T14925 7733-7734 . denotes .
T14926 7735-7738 DT denotes The
T14927 7739-7745 NNP denotes SV40pA
T14928 7746-7754 NN denotes sequence
T14929 7755-7760 VBZ denotes helps
T14930 7761-7763 TO denotes to
T14931 7764-7770 VB denotes ensure
T14932 7771-7781 NN denotes disruption
T14933 7782-7784 IN denotes of
T14934 7785-7789 NN denotes gene
T14935 7790-7800 NN denotes expression
T14936 7801-7806 IN denotes after
T14937 7807-7815 NN denotes deletion
T14938 7816-7818 IN denotes of
T14939 7819-7822 DT denotes the
T14940 7823-7827 NNP denotes NeoR
T14941 7828-7836 NN denotes sequence
T14942 7836-7837 . denotes .
T14943 7838-7839 -LRB- denotes (
T14944 7839-7840 NNP denotes B
T14945 7840-7841 -RRB- denotes )
T14946 7842-7851 NNP denotes Anti-HOIP
T14947 7852-7855 CC denotes and
T14948 7856-7865 NNP denotes anti-FLAG
T14949 7866-7873 NNP denotes Western
T14950 7874-7879 NNS denotes blots
T14951 7880-7882 IN denotes of
T14952 7883-7887 NN denotes cell
T14953 7888-7895 NNS denotes lysates
T14954 7896-7900 IN denotes from
T14955 7901-7904 NNP denotes A20
T14956 7904-7906 CD denotes .2
T14957 7906-7907 NNP denotes J
T14958 7908-7913 NNS denotes cells
T14959 7914-7917 CC denotes and
T14960 7918-7932 JJ denotes HOIP-deficient
T14961 7933-7934 -LRB- denotes (
T14962 7934-7938 SYM denotes HOIP
T14963 7938-7939 : denotes -
T14964 7939-7940 SYM denotes /
T14965 7940-7941 : denotes -
T14966 7941-7942 -RRB- denotes )
T14967 7943-7949 NNS denotes clones
T14968 7949-7950 . denotes .
T14969 7951-7952 DT denotes A
T14970 7953-7960 JJ denotes partial
T14971 7961-7969 NN denotes decrease
T14972 7970-7972 IN denotes in
T14973 7973-7977 NNP denotes HOIP
T14974 7978-7985 NN denotes protein
T14975 7986-7996 NN denotes expression
T14976 7997-7999 VBZ denotes is
T14977 8000-8007 JJ denotes evident
T14978 8008-8010 IN denotes in
T14979 8011-8016 NNS denotes cells
T14980 8017-8026 VBG denotes following
T14981 8027-8037 NN denotes disruption
T14982 8038-8040 IN denotes of
T14983 8041-8044 CD denotes one
T14984 8045-8049 NN denotes copy
T14985 8050-8052 IN denotes of
T14986 8053-8058 NNP denotes Rnf31
T14987 8059-8060 -LRB- denotes (
T14988 8060-8064 NNP denotes HOIP
T14989 8064-8065 CD denotes −
T14990 8065-8066 CD denotes /
T14991 8066-8067 NN denotes +
T14992 8067-8068 -RRB- denotes )
T14993 8068-8069 . denotes .
T14994 8070-8074 NNP denotes HOIP
T14995 8075-8085 NN denotes expression
T14996 8086-8088 IN denotes in
T14997 8089-8095 NNS denotes clones
T14998 8096-8109 VBN denotes reconstituted
T14999 8110-8114 IN denotes with
T15000 8115-8117 DT denotes an
T15001 8118-8123 JJ denotes empty
T15002 8124-8134 NN denotes retroviral
T15003 8135-8141 NN denotes vector
T15004 8142-8143 -LRB- denotes (
T15005 8143-8147 NNP denotes pMIP
T15006 8147-8148 -RRB- denotes )
T15007 8149-8151 CC denotes or
T15008 8152-8153 DT denotes a
T15009 8154-8164 JJ denotes retroviral
T15010 8165-8171 NN denotes vector
T15011 8172-8180 VBG denotes encoding
T15012 8181-8192 JJ denotes FLAG-tagged
T15013 8193-8197 NNP denotes HOIP
T15014 8198-8200 VBZ denotes is
T15015 8201-8205 RB denotes also
T15016 8206-8211 VBN denotes shown
T15017 8211-8212 . denotes .
T15018 8213-8224 JJ denotes Approximate
T15019 8225-8234 JJ denotes molecular
T15020 8235-8241 NN denotes weight
T15021 8242-8244 IN denotes of
T15022 8245-8249 NNP denotes HOIP
T15023 8250-8252 VBZ denotes is
T15024 8253-8256 CD denotes 120
T15025 8257-8259 NN denotes kD
T15026 8259-8260 . denotes .
T3245 8263-8265 TO denotes To
T3246 8266-8272 VB denotes enable
T3247 8273-8275 PRP denotes us
T3248 8276-8278 TO denotes to
T3249 8279-8286 VB denotes confirm
T3250 8287-8291 IN denotes that
T3251 8292-8295 DT denotes any
T3252 8296-8305 VBG denotes signaling
T3253 8306-8308 CC denotes or
T3254 8309-8319 JJ denotes functional
T3255 8320-8327 NNS denotes defects
T3256 8328-8336 VBD denotes observed
T3257 8337-8339 IN denotes in
T3258 8340-8354 JJ denotes HOIP-deficient
T3259 8355-8360 NNS denotes cells
T3260 8361-8365 VBD denotes were
T3261 8366-8369 JJ denotes due
T3262 8370-8372 TO denotes to
T3263 8373-8383 NN denotes disruption
T3264 8384-8386 IN denotes of
T3265 8387-8390 DT denotes the
T3266 8391-8396 NNP denotes Rnf31
T3267 8397-8401 NN denotes gene
T3268 8401-8402 , denotes ,
T3269 8403-8407 DT denotes both
T3270 8408-8422 JJ denotes HOIP-deficient
T3271 8423-8427 NN denotes cell
T3272 8428-8433 NNS denotes lines
T3273 8434-8438 VBD denotes were
T3274 8439-8449 VBN denotes transduced
T3275 8450-8454 IN denotes with
T3276 8455-8456 DT denotes a
T3277 8457-8467 NN denotes retrovirus
T3278 8468-8476 VBG denotes encoding
T3279 8477-8488 JJ denotes FLAG-tagged
T3280 8489-8498 JJ denotes wild-type
T3281 8499-8503 NNP denotes HOIP
T3282 8503-8504 , denotes ,
T3283 8505-8509 RB denotes thus
T3284 8510-8519 VBG denotes restoring
T3285 8520-8524 NNP denotes HOIP
T3286 8525-8532 NN denotes protein
T3287 8533-8543 NN denotes expression
T3288 8544-8545 -LRB- denotes (
T3289 8545-8549 NNP denotes Fig.
T3290 8550-8552 NNP denotes 1B
T3291 8552-8553 -RRB- denotes )
T3292 8553-8554 . denotes .
T3293 8555-8560 DT denotes These
T3294 8561-8566 NNS denotes cells
T3295 8567-8570 VBP denotes are
T3296 8571-8579 VBN denotes referred
T3297 8580-8582 TO denotes to
T3298 8583-8585 IN denotes as
T3299 8586-8604 JJ denotes HOIP-reconstituted
T3300 8605-8610 NNS denotes cells
T3301 8610-8611 . denotes .
T3302 8612-8614 IN denotes In
T3303 8615-8618 DT denotes the
T3304 8619-8630 NNS denotes experiments
T3305 8631-8635 WDT denotes that
T3306 8636-8642 VBP denotes follow
T3307 8642-8643 , denotes ,
T3308 8644-8653 NNS denotes responses
T3309 8654-8656 IN denotes by
T3310 8657-8662 DT denotes these
T3311 8663-8668 NNS denotes cells
T3312 8669-8673 VBD denotes were
T3313 8674-8682 VBN denotes compared
T3314 8683-8685 TO denotes to
T3315 8686-8691 DT denotes those
T3316 8692-8694 IN denotes of
T3317 8695-8698 NNP denotes A20
T3318 8698-8700 CD denotes .2
T3319 8700-8701 NNP denotes J
T3320 8702-8707 NNS denotes cells
T3321 8708-8711 CC denotes and
T3322 8712-8726 JJ denotes HOIP-deficient
T3323 8727-8732 NNS denotes cells
T3324 8733-8743 VBN denotes transduced
T3325 8744-8748 IN denotes with
T3326 8749-8750 DT denotes a
T3327 8751-8761 JJ denotes retroviral
T3328 8762-8768 NN denotes vector
T3329 8769-8770 -LRB- denotes (
T3330 8770-8774 NNP denotes pMIP
T3331 8774-8775 -RRB- denotes )
T3332 8776-8783 VBG denotes lacking
T3333 8784-8785 DT denotes a
T3334 8786-8790 NNP denotes cDNA
T3335 8791-8797 NN denotes insert
T3336 8798-8799 -LRB- denotes (
T3337 8799-8804 JJ denotes empty
T3338 8805-8811 NN denotes vector
T3339 8811-8812 -RRB- denotes )
T3340 8812-8813 . denotes .
T4259 8815-8819 NNP denotes HOIP
T4260 8820-8822 VBZ denotes is
T4261 8823-8831 VBN denotes required
T4262 8832-8835 IN denotes for
T4263 8836-8849 JJ denotes CD40-mediated
T4264 8850-8854 NN denotes CD80
T4265 8855-8867 NN denotes upregulation
T4266 8868-8871 CC denotes and
T4267 8872-8882 NN denotes activation
T4268 8883-8885 IN denotes of
T4269 8886-8894 NN denotes germline
T4270 8895-8902 NN denotes epsilon
T4271 8903-8916 NN denotes transcription
T4272 8917-8927 NNP denotes Engagement
T4273 8928-8930 IN denotes of
T4274 8931-8935 NNP denotes CD40
T4275 8936-8938 IN denotes on
T4276 8939-8940 NNP denotes B
T4277 8941-8946 NNS denotes cells
T4278 8947-8958 VBZ denotes upregulates
T4279 8959-8969 NN denotes expression
T4280 8970-8972 IN denotes of
T4281 8973-8977 NNP denotes CD80
T4282 8978-8979 NNP denotes [
T4283 8979-8981 CD denotes 10
T4284 8981-8982 NNP denotes ]
T4285 8982-8983 , denotes ,
T4286 8984-8985 DT denotes a
T4287 8986-8990 NN denotes cell
T4288 8991-8998 NN denotes surface
T4289 8999-9006 NN denotes protein
T4290 9007-9011 WDT denotes that
T4291 9012-9020 VBZ denotes promotes
T4292 9021-9031 NN denotes activation
T4293 9032-9034 IN denotes of
T4294 9035-9036 NNP denotes T
T4295 9037-9042 NNS denotes cells
T4296 9043-9054 VBG denotes interacting
T4297 9055-9059 IN denotes with
T4298 9060-9061 NNP denotes B
T4299 9062-9067 NNS denotes cells
T4300 9068-9071 CC denotes and
T4301 9072-9077 JJ denotes other
T4302 9078-9081 NNP denotes APC
T4303 9081-9082 . denotes .
T4304 9083-9085 TO denotes To
T4305 9086-9091 VB denotes begin
T4306 9092-9102 VBG denotes evaluating
T4307 9103-9112 JJ denotes potential
T4308 9113-9126 NNS denotes contributions
T4309 9127-9129 IN denotes of
T4310 9130-9134 NNP denotes HOIP
T4311 9135-9137 TO denotes to
T4312 9138-9142 NNP denotes CD40
T4313 9143-9152 VBG denotes signaling
T4314 9152-9153 , denotes ,
T4315 9154-9156 PRP denotes we
T4316 9157-9164 VBD denotes assayed
T4317 9165-9168 DT denotes the
T4318 9169-9181 NN denotes upregulation
T4319 9182-9184 IN denotes of
T4320 9185-9189 CD denotes CD80
T4321 9190-9197 VBN denotes induced
T4322 9198-9200 IN denotes by
T4323 9201-9211 NN denotes engagement
T4324 9212-9214 IN denotes of
T4325 9215-9219 NNP denotes CD40
T4326 9220-9222 IN denotes on
T4327 9223-9231 JJ denotes parental
T4328 9232-9235 NNP denotes A20
T4329 9235-9237 CD denotes .2
T4330 9237-9238 NNP denotes J
T4331 9238-9239 , denotes ,
T4332 9240-9254 NNP denotes HOIP-deficient
T4333 9254-9255 , denotes ,
T4334 9256-9259 CC denotes and
T4335 9260-9278 JJ denotes HOIP-reconstituted
T4336 9279-9284 NNS denotes cells
T4337 9285-9286 -LRB- denotes (
T4338 9286-9290 JJ denotes Fig.
T4339 9291-9292 CD denotes 2
T4340 9292-9293 -RRB- denotes )
T4341 9293-9294 . denotes .
T4342 9295-9303 VBN denotes Compared
T4343 9304-9306 TO denotes to
T4344 9307-9310 DT denotes the
T4345 9311-9319 JJ denotes parental
T4346 9320-9324 NN denotes cell
T4347 9325-9329 NN denotes line
T4348 9329-9330 , denotes ,
T4349 9331-9334 DT denotes the
T4350 9335-9350 JJ denotes CD40-stimulated
T4351 9351-9363 NN denotes upregulation
T4352 9364-9366 IN denotes of
T4353 9367-9371 NN denotes CD80
T4354 9372-9375 VBD denotes was
T4355 9376-9388 RB denotes dramatically
T4356 9389-9396 VBN denotes reduced
T4357 9397-9399 IN denotes in
T4358 9400-9403 DT denotes all
T4359 9404-9418 JJ denotes HOIP-deficient
T4360 9419-9424 NNS denotes cells
T4361 9425-9431 VBN denotes tested
T4362 9431-9432 . denotes .
T4363 9433-9435 IN denotes In
T4364 9436-9444 NN denotes contrast
T4365 9444-9445 , denotes ,
T4366 9446-9458 NN denotes upregulation
T4367 9459-9461 IN denotes of
T4368 9462-9466 NN denotes CD80
T4369 9467-9470 VBD denotes was
T4370 9471-9479 VBN denotes restored
T4371 9480-9482 IN denotes in
T4372 9483-9501 JJ denotes HOIP-reconstituted
T4373 9502-9507 NNS denotes cells
T4374 9507-9508 , denotes ,
T4375 9509-9519 VBG denotes confirming
T4376 9520-9524 IN denotes that
T4377 9525-9528 DT denotes the
T4378 9529-9535 NN denotes defect
T4379 9536-9538 IN denotes in
T4380 9539-9543 NNP denotes CD80
T4381 9544-9556 NN denotes upregulation
T4382 9557-9559 IN denotes in
T4383 9560-9563 DT denotes the
T4384 9564-9578 JJ denotes HOIP-deficient
T4385 9579-9584 NNS denotes cells
T4386 9585-9588 VBD denotes was
T4387 9589-9592 JJ denotes due
T4388 9593-9595 TO denotes to
T4389 9596-9599 DT denotes the
T4390 9600-9610 NN denotes disruption
T4391 9611-9613 IN denotes of
T4392 9614-9619 NNP denotes Rnf31
T4393 9619-9620 . denotes .
T15392 9665-9678 JJ denotes CD40-mediated
T15393 9679-9683 NN denotes CD80
T15394 9684-9696 NN denotes upregulation
T15395 9697-9699 VBZ denotes is
T15396 9700-9709 JJ denotes defective
T15397 9710-9712 IN denotes in
T15398 9713-9727 JJ denotes HOIP-deficient
T15399 9728-9733 NNS denotes cells
T15400 9733-9734 . denotes .
T15401 9735-9740 NNS denotes Cells
T15402 9741-9745 VBD denotes were
T15403 9746-9755 VBN denotes incubated
T15404 9756-9759 IN denotes for
T15405 9760-9762 CD denotes 72
T15406 9763-9766 NNS denotes hrs
T15407 9767-9771 IN denotes with
T15408 9772-9774 DT denotes an
T15409 9775-9782 NN denotes isotype
T15410 9783-9790 NN denotes control
T15411 9791-9799 NN denotes antibody
T15412 9800-9801 -LRB- denotes (
T15413 9801-9805 VBN denotes left
T15414 9805-9806 -RRB- denotes )
T15415 9807-9809 CC denotes or
T15416 9810-9819 JJ denotes anti-CD40
T15417 9820-9828 NN denotes antibody
T15418 9829-9830 -LRB- denotes (
T15419 9830-9835 NN denotes right
T15420 9835-9836 -RRB- denotes )
T15421 9837-9840 CC denotes and
T15422 9841-9845 RB denotes then
T15423 9846-9853 VBD denotes stained
T15424 9854-9857 IN denotes for
T15425 9858-9868 NN denotes expression
T15426 9869-9871 IN denotes of
T15427 9872-9876 NNP denotes CD80
T15428 9877-9878 -LRB- denotes (
T15429 9878-9884 VBN denotes filled
T15430 9885-9893 NNS denotes profiles
T15431 9894-9902 VBP denotes indicate
T15432 9903-9911 VBG denotes staining
T15433 9912-9916 IN denotes with
T15434 9917-9926 JJ denotes anti-CD80
T15435 9927-9935 NN denotes antibody
T15436 9935-9936 : denotes ;
T15437 9937-9941 JJ denotes open
T15438 9942-9950 NNS denotes profiles
T15439 9951-9954 VBP denotes are
T15440 9955-9963 VBG denotes staining
T15441 9964-9968 IN denotes with
T15442 9969-9971 DT denotes an
T15443 9972-9979 NN denotes isotype
T15444 9980-9987 NN denotes control
T15445 9988-9996 NN denotes antibody
T15446 9996-9997 -RRB- denotes )
T15447 9997-9998 . denotes .
T15448 9999-10011 NNP denotes Transduction
T15449 10012-10014 IN denotes of
T15450 10015-10019 DT denotes both
T15451 10020-10034 JJ denotes HOIP-deficient
T15452 10035-10036 -LRB- denotes (
T15453 10036-10040 SYM denotes HOIP
T15454 10040-10041 : denotes -
T15455 10041-10042 SYM denotes /
T15456 10042-10043 : denotes -
T15457 10043-10044 -RRB- denotes )
T15458 10045-10051 NNS denotes clones
T15459 10052-10056 IN denotes with
T15460 10057-10058 DT denotes a
T15461 10059-10069 NN denotes retrovirus
T15462 10070-10078 VBG denotes encoding
T15463 10079-10088 NNP denotes FLAG-HOIP
T15464 10089-10097 VBD denotes reversed
T15465 10098-10107 JJ denotes defective
T15466 10108-10112 NNP denotes CD80
T15467 10113-10125 NN denotes upregulation
T15468 10125-10126 , denotes ,
T15469 10127-10132 IN denotes while
T15470 10133-10145 NN denotes transduction
T15471 10146-10150 IN denotes with
T15472 10151-10153 DT denotes an
T15473 10154-10159 JJ denotes empty
T15474 10160-10166 NN denotes vector
T15475 10167-10168 -LRB- denotes (
T15476 10168-10172 NNP denotes pMIP
T15477 10172-10173 -RRB- denotes )
T15478 10174-10177 VBD denotes did
T15479 10178-10181 RB denotes not
T15480 10181-10182 . denotes .
T15481 10183-10190 JJ denotes Similar
T15482 10191-10198 NNS denotes results
T15483 10199-10203 VBD denotes were
T15484 10204-10212 VBN denotes obtained
T15485 10213-10215 IN denotes in
T15486 10216-10217 DT denotes a
T15487 10218-10224 JJ denotes second
T15488 10225-10235 NN denotes experiment
T15489 10235-10236 . denotes .
T4394 10239-10243 CD denotes CD40
T4395 10244-10251 NNS denotes signals
T4396 10252-10254 IN denotes in
T4397 10255-10256 NNP denotes B
T4398 10257-10262 NNS denotes cells
T4399 10263-10266 MD denotes can
T4400 10267-10271 RB denotes also
T4401 10272-10282 VB denotes contribute
T4402 10283-10285 TO denotes to
T4403 10286-10289 DT denotes the
T4404 10290-10300 NN denotes activation
T4405 10301-10303 IN denotes of
T4406 10304-10307 NNP denotes DNA
T4407 10308-10321 NN denotes recombination
T4408 10322-10324 IN denotes in
T4409 10325-10328 DT denotes the
T4410 10329-10343 NN denotes immunoglobulin
T4411 10344-10349 JJ denotes heavy
T4412 10350-10355 NN denotes chain
T4413 10356-10361 NN denotes locus
T4414 10362-10363 NNP denotes [
T4415 10363-10365 CD denotes 11
T4416 10365-10366 NNP denotes ]
T4417 10366-10367 , denotes ,
T4418 10368-10369 NNP denotes [
T4419 10369-10371 CD denotes 12
T4420 10371-10372 NNP denotes ]
T4421 10372-10373 . denotes .
T4422 10374-10378 DT denotes This
T4423 10379-10386 NN denotes process
T4424 10386-10387 , denotes ,
T4425 10388-10393 VBN denotes known
T4426 10394-10396 IN denotes as
T4427 10397-10411 JJ denotes immunoglobulin
T4428 10412-10417 NN denotes class
T4429 10418-10427 NN denotes switching
T4430 10428-10429 -LRB- denotes (
T4431 10429-10431 CC denotes or
T4432 10432-10439 JJ denotes isotype
T4433 10440-10449 NN denotes switching
T4434 10449-10450 -RRB- denotes )
T4435 10450-10451 , denotes ,
T4436 10452-10458 VBZ denotes allows
T4437 10459-10460 NNP denotes B
T4438 10461-10466 NNS denotes cells
T4439 10466-10467 , denotes ,
T4440 10468-10477 RB denotes initially
T4441 10478-10488 VBG denotes expressing
T4442 10489-10492 NNP denotes IgM
T4443 10492-10493 , denotes ,
T4444 10494-10496 TO denotes to
T4445 10497-10503 VB denotes switch
T4446 10504-10506 TO denotes to
T4447 10507-10510 NNP denotes IgA
T4448 10510-10511 , denotes ,
T4449 10512-10515 NNP denotes IgG
T4450 10515-10516 , denotes ,
T4451 10517-10519 CC denotes or
T4452 10520-10523 NNP denotes IgE
T4453 10524-10533 VBG denotes depending
T4454 10534-10536 IN denotes on
T4455 10537-10540 DT denotes the
T4456 10541-10546 NN denotes class
T4457 10547-10549 IN denotes of
T4458 10550-10558 NN denotes antibody
T4459 10559-10563 RBS denotes most
T4460 10564-10575 JJ denotes appropriate
T4461 10576-10579 IN denotes for
T4462 10580-10581 DT denotes a
T4463 10582-10592 JJ denotes particular
T4464 10593-10599 JJ denotes immune
T4465 10600-10608 NN denotes response
T4466 10608-10609 . denotes .
T4467 10610-10619 VBG denotes Switching
T4468 10620-10622 TO denotes to
T4469 10623-10626 NNP denotes IgE
T4470 10627-10630 MD denotes can
T4471 10631-10633 VB denotes be
T4472 10634-10641 VBN denotes induced
T4473 10642-10644 IN denotes by
T4474 10645-10648 DT denotes the
T4475 10649-10660 NN denotes combination
T4476 10661-10663 IN denotes of
T4477 10664-10668 NN denotes IL-4
T4478 10669-10672 CC denotes and
T4479 10673-10677 JJ denotes CD40
T4480 10678-10685 NNS denotes signals
T4481 10685-10686 . denotes .
T4482 10687-10690 DT denotes The
T4483 10691-10695 NN denotes gene
T4484 10696-10709 NN denotes rearrangement
T4485 10710-10719 JJ denotes necessary
T4486 10720-10723 IN denotes for
T4487 10724-10727 JJ denotes IgE
T4488 10728-10738 NN denotes production
T4489 10739-10741 VBZ denotes is
T4490 10742-10750 VBN denotes preceded
T4491 10751-10753 IN denotes by
T4492 10754-10757 DT denotes the
T4493 10758-10768 NN denotes production
T4494 10769-10771 IN denotes of
T4495 10772-10782 JJ denotes non-coding
T4496 10783-10786 NNP denotes RNA
T4497 10787-10798 NNS denotes transcripts
T4498 10799-10803 IN denotes from
T4499 10804-10812 JJ denotes germline
T4500 10813-10822 NNS denotes sequences
T4501 10823-10825 IN denotes in
T4502 10826-10829 DT denotes the
T4503 10830-10833 NNP denotes IgE
T4504 10834-10839 NN denotes heavy
T4505 10840-10845 NN denotes chain
T4506 10846-10851 VBZ denotes locus
T4507 10852-10853 NNP denotes [
T4508 10853-10855 CD denotes 13
T4509 10855-10856 NNP denotes ]
T4510 10856-10857 . denotes .
T4511 10858-10860 TO denotes To
T4512 10861-10870 VB denotes determine
T4513 10871-10878 IN denotes whether
T4514 10879-10883 NNP denotes HOIP
T4515 10884-10886 VBZ denotes is
T4516 10887-10895 VBN denotes required
T4517 10896-10898 IN denotes in
T4518 10899-10902 DT denotes the
T4519 10903-10913 NN denotes initiation
T4520 10914-10916 IN denotes of
T4521 10917-10920 DT denotes the
T4522 10921-10926 NN denotes class
T4523 10927-10936 VBG denotes switching
T4524 10937-10944 NN denotes process
T4525 10944-10945 , denotes ,
T4526 10946-10949 DT denotes the
T4527 10950-10958 JJ denotes relevant
T4528 10959-10960 NNP denotes B
T4529 10961-10965 NN denotes cell
T4530 10966-10971 NNS denotes lines
T4531 10972-10976 VBD denotes were
T4532 10977-10987 VBN denotes stimulated
T4533 10988-10995 IN denotes through
T4534 10996-11000 NNP denotes CD40
T4535 11001-11003 IN denotes in
T4536 11004-11007 DT denotes the
T4537 11008-11016 NN denotes presence
T4538 11017-11019 IN denotes of
T4539 11020-11024 NN denotes IL-4
T4540 11025-11028 CC denotes and
T4541 11029-11035 VBN denotes tested
T4542 11036-11039 IN denotes for
T4543 11040-11050 NN denotes production
T4544 11051-11053 IN denotes of
T4545 11054-11062 NN denotes germline
T4546 11063-11070 NN denotes epsilon
T4547 11071-11072 -LRB- denotes (
T4548 11072-11075 NNP denotes GLε
T4549 11075-11076 -RRB- denotes )
T4550 11077-11088 NNS denotes transcripts
T4551 11089-11090 -LRB- denotes (
T4552 11090-11094 JJ denotes Fig.
T4553 11095-11096 CD denotes 3
T4554 11096-11097 -RRB- denotes )
T4555 11097-11098 . denotes .
T4556 11099-11108 JJ denotes Anti-CD40
T4557 11109-11117 NN denotes antibody
T4558 11118-11129 NN denotes stimulation
T4559 11130-11135 RB denotes alone
T4560 11136-11138 CC denotes or
T4561 11139-11147 RB denotes together
T4562 11148-11152 IN denotes with
T4563 11153-11157 NN denotes IL-4
T4564 11158-11165 VBD denotes induced
T4565 11166-11169 NNP denotes GLε
T4566 11170-11183 NN denotes transcription
T4567 11184-11186 IN denotes in
T4568 11187-11195 JJ denotes parental
T4569 11196-11199 NNP denotes A20
T4570 11199-11201 CD denotes .2
T4571 11201-11202 NNP denotes J
T4572 11203-11208 NNS denotes cells
T4573 11209-11211 IN denotes as
T4574 11212-11220 VBN denotes expected
T4575 11220-11221 . denotes .
T4576 11222-11224 IN denotes In
T4577 11225-11233 NN denotes contrast
T4578 11233-11234 , denotes ,
T4579 11235-11239 RB denotes only
T4580 11240-11243 JJ denotes low
T4581 11244-11250 NNS denotes levels
T4582 11251-11253 IN denotes of
T4583 11254-11257 NNP denotes GLε
T4584 11258-11268 NN denotes expression
T4585 11269-11273 VBD denotes were
T4586 11274-11282 VBN denotes detected
T4587 11283-11285 IN denotes in
T4588 11286-11300 JJ denotes HOIP-deficient
T4589 11301-11306 NNS denotes cells
T4590 11307-11317 VBN denotes stimulated
T4591 11318-11322 IN denotes with
T4592 11323-11329 DT denotes either
T4593 11330-11339 JJ denotes anti-CD40
T4594 11340-11348 NN denotes antibody
T4595 11349-11354 RB denotes alone
T4596 11355-11357 CC denotes or
T4597 11358-11367 JJ denotes anti-CD40
T4598 11368-11371 CC denotes and
T4599 11372-11376 NN denotes IL-4
T4600 11376-11377 . denotes .
T4601 11378-11384 JJ denotes Normal
T4602 11385-11391 NNS denotes levels
T4603 11392-11394 IN denotes of
T4604 11395-11398 NNP denotes GLε
T4605 11399-11409 NN denotes expression
T4606 11410-11414 VBD denotes were
T4607 11415-11422 VBN denotes induced
T4608 11423-11425 IN denotes in
T4609 11426-11444 JJ denotes HOIP-reconstituted
T4610 11445-11450 NNS denotes cells
T4611 11451-11461 VBN denotes stimulated
T4612 11462-11466 IN denotes with
T4613 11467-11476 JJ denotes anti-CD40
T4614 11477-11485 NN denotes antibody
T4615 11486-11491 RB denotes alone
T4616 11492-11494 CC denotes or
T4617 11495-11503 RB denotes together
T4618 11504-11508 IN denotes with
T4619 11509-11513 NN denotes IL-4
T4620 11513-11514 . denotes .
T4621 11515-11527 RB denotes Collectively
T4622 11527-11528 , denotes ,
T4623 11529-11534 DT denotes these
T4624 11535-11539 NNS denotes data
T4625 11540-11551 VBP denotes demonstrate
T4626 11552-11556 IN denotes that
T4627 11557-11561 NNP denotes HOIP
T4628 11562-11565 VBZ denotes has
T4629 11566-11567 DT denotes a
T4630 11568-11576 JJ denotes critical
T4631 11577-11581 NN denotes role
T4632 11582-11584 IN denotes in
T4633 11585-11593 JJ denotes cellular
T4634 11594-11603 NNS denotes responses
T4635 11604-11606 TO denotes to
T4636 11607-11611 CD denotes CD40
T4637 11612-11621 VBG denotes signaling
T4638 11621-11622 . denotes .
T15742 11667-11670 NNP denotes GLε
T15743 11671-11684 NN denotes transcription
T15744 11685-11687 VBZ denotes is
T15745 11688-11697 JJ denotes defective
T15746 11698-11700 IN denotes in
T15747 11701-11716 JJ denotes CD40-stimulated
T15748 11717-11731 JJ denotes HOIP-deficient
T15749 11732-11737 NNS denotes cells
T15750 11737-11738 . denotes .
T15751 11739-11742 CD denotes A20
T15752 11742-11744 CD denotes .2
T15753 11744-11745 NNP denotes J
T15754 11746-11749 CC denotes and
T15755 11750-11764 JJ denotes HOIP-deficient
T15756 11765-11770 NNS denotes cells
T15757 11771-11781 VBN denotes transduced
T15758 11782-11786 IN denotes with
T15759 11787-11789 DT denotes an
T15760 11790-11795 JJ denotes empty
T15761 11796-11806 NN denotes retroviral
T15762 11807-11813 NN denotes vector
T15763 11814-11815 -LRB- denotes (
T15764 11815-11818 CD denotes A20
T15765 11818-11820 CD denotes .2
T15766 11820-11821 NNP denotes J
T15767 11822-11823 CD denotes +
T15768 11824-11828 NNP denotes pMIP
T15769 11829-11832 CC denotes and
T15770 11833-11837 NNP denotes HOIP
T15771 11837-11838 : denotes -
T15772 11838-11839 SYM denotes /
T15773 11839-11840 : denotes -
T15774 11841-11842 NN denotes +
T15775 11843-11847 NNP denotes pMIP
T15776 11847-11848 , denotes ,
T15777 11849-11861 RB denotes respectively
T15778 11861-11862 -RRB- denotes )
T15779 11862-11863 , denotes ,
T15780 11864-11866 CC denotes or
T15781 11867-11881 JJ denotes HOIP-deficient
T15782 11882-11887 NNS denotes cells
T15783 11888-11898 VBN denotes transduced
T15784 11899-11903 IN denotes with
T15785 11904-11905 DT denotes a
T15786 11906-11919 JJ denotes HOIP-encoding
T15787 11920-11930 NN denotes retrovirus
T15788 11931-11932 -LRB- denotes (
T15789 11932-11936 SYM denotes HOIP
T15790 11936-11937 : denotes -
T15791 11937-11938 SYM denotes /
T15792 11938-11939 : denotes -
T15793 11940-11941 NN denotes +
T15794 11942-11946 NNP denotes HOIP
T15795 11946-11947 -RRB- denotes )
T15796 11948-11952 VBD denotes were
T15797 11953-11961 VBN denotes cultured
T15798 11962-11971 JJ denotes overnight
T15799 11972-11976 IN denotes with
T15800 11977-11986 JJ denotes agonistic
T15801 11987-11996 JJ denotes anti-CD40
T15802 11997-11999 CC denotes or
T15803 12000-12002 DT denotes an
T15804 12003-12010 NN denotes isotype
T15805 12011-12018 NN denotes control
T15806 12019-12027 NN denotes antibody
T15807 12028-12029 -LRB- denotes (
T15808 12029-12032 FW denotes iso
T15809 12032-12033 -RRB- denotes )
T15810 12033-12034 , denotes ,
T15811 12035-12039 IN denotes with
T15812 12040-12042 CC denotes or
T15813 12043-12050 IN denotes without
T15814 12051-12055 NN denotes IL-4
T15815 12055-12056 . denotes .
T15816 12057-12060 NNP denotes RNA
T15817 12061-12064 VBD denotes was
T15818 12065-12073 VBN denotes isolated
T15819 12074-12078 IN denotes from
T15820 12079-12082 DT denotes the
T15821 12083-12088 NNS denotes cells
T15822 12088-12089 , denotes ,
T15823 12090-12109 JJ denotes reverse-transcribed
T15824 12109-12110 , denotes ,
T15825 12111-12114 CC denotes and
T15826 12115-12119 RB denotes then
T15827 12120-12129 VBN denotes subjected
T15828 12130-12132 TO denotes to
T15829 12133-12145 JJ denotes quantitative
T15830 12146-12149 NNP denotes PCR
T15831 12150-12152 TO denotes to
T15832 12153-12162 VB denotes determine
T15833 12163-12169 NNS denotes levels
T15834 12170-12172 IN denotes of
T15835 12173-12176 NNP denotes GLε
T15836 12177-12188 NNS denotes transcripts
T15837 12188-12189 . denotes .
T15838 12190-12197 NNS denotes Results
T15839 12198-12202 VBD denotes were
T15840 12203-12213 VBN denotes normalized
T15841 12214-12216 TO denotes to
T15842 12217-12220 DT denotes the
T15843 12221-12227 NNS denotes levels
T15844 12228-12230 IN denotes of
T15845 12231-12236 JJ denotes Hprt1
T15846 12237-12248 NNS denotes transcripts
T15847 12249-12251 IN denotes in
T15848 12252-12256 DT denotes each
T15849 12257-12263 NN denotes sample
T15850 12263-12264 . denotes .
T15851 12265-12272 NNS denotes Symbols
T15852 12273-12281 VBP denotes indicate
T15853 12282-12285 DT denotes the
T15854 12286-12292 NNS denotes values
T15855 12293-12297 IN denotes from
T15856 12298-12307 VBP denotes duplicate
T15857 12308-12316 NNS denotes cultures
T15858 12317-12318 -LRB- denotes (
T15859 12318-12319 DT denotes a
T15860 12320-12324 NN denotes line
T15861 12325-12334 VBZ denotes indicates
T15862 12335-12338 DT denotes the
T15863 12339-12343 NN denotes mean
T15864 12344-12346 IN denotes of
T15865 12347-12350 DT denotes the
T15866 12351-12354 CD denotes two
T15867 12355-12361 NNS denotes values
T15868 12361-12362 -RRB- denotes )
T15869 12362-12363 . denotes .
T15870 12364-12371 JJ denotes Similar
T15871 12372-12379 NNS denotes results
T15872 12380-12384 VBD denotes were
T15873 12385-12393 VBN denotes obtained
T15874 12394-12396 IN denotes in
T15875 12397-12398 DT denotes a
T15876 12399-12405 JJ denotes second
T15877 12406-12416 NN denotes experiment
T15878 12417-12420 CC denotes and
T15879 12421-12423 IN denotes in
T15880 12424-12426 DT denotes an
T15881 12427-12437 JJ denotes additional
T15882 12438-12448 NN denotes experiment
T15883 12449-12453 IN denotes with
T15884 12454-12455 DT denotes a
T15885 12456-12462 JJ denotes second
T15886 12463-12477 JJ denotes HOIP-deficient
T15887 12478-12483 NN denotes clone
T15888 12483-12484 . denotes .
T5508 12486-12490 NNP denotes HOIP
T5509 12491-12499 VBZ denotes mediates
T5510 12500-12515 JJ denotes CD40-stimulated
T5511 12516-12521 NN denotes NF-κB
T5512 12522-12525 CC denotes and
T5513 12526-12529 NNP denotes JNK
T5514 12530-12540 NN denotes activation
T5515 12541-12544 DT denotes The
T5516 12545-12552 NNS denotes results
T5517 12553-12562 VBN denotes described
T5518 12563-12568 IN denotes above
T5519 12569-12573 NN denotes show
T5520 12574-12578 IN denotes that
T5521 12579-12583 NNP denotes HOIP
T5522 12584-12589 VBZ denotes plays
T5523 12590-12592 DT denotes an
T5524 12593-12602 JJ denotes important
T5525 12603-12607 NN denotes role
T5526 12608-12610 IN denotes in
T5527 12611-12624 JJ denotes CD40-mediated
T5528 12625-12633 NN denotes effector
T5529 12634-12643 NNS denotes functions
T5530 12644-12646 IN denotes of
T5531 12647-12648 NNP denotes B
T5532 12649-12654 NNS denotes cells
T5533 12654-12655 . denotes .
T5534 12656-12658 PRP denotes It
T5535 12659-12666 VBZ denotes follows
T5536 12667-12671 IN denotes that
T5537 12672-12676 NNP denotes HOIP
T5538 12677-12679 VBZ denotes is
T5539 12680-12686 JJ denotes likely
T5540 12687-12688 DT denotes a
T5541 12689-12692 JJ denotes key
T5542 12693-12701 NN denotes mediator
T5543 12702-12704 IN denotes of
T5544 12705-12709 NNP denotes CD40
T5545 12710-12719 VBG denotes signaling
T5546 12719-12720 . denotes .
T5547 12721-12723 TO denotes To
T5548 12724-12728 VB denotes test
T5549 12729-12733 DT denotes this
T5550 12734-12744 NN denotes hypothesis
T5551 12744-12745 , denotes ,
T5552 12746-12748 PRP denotes we
T5553 12749-12759 VBD denotes stimulated
T5554 12760-12763 NNP denotes A20
T5555 12763-12765 CD denotes .2
T5556 12765-12766 NNP denotes J
T5557 12767-12770 CC denotes and
T5558 12771-12785 JJ denotes HOIP-deficient
T5559 12786-12791 NNS denotes cells
T5560 12792-12796 IN denotes with
T5561 12797-12802 NNP denotes CD154
T5562 12803-12804 -LRB- denotes (
T5563 12804-12808 NNP denotes CD40
T5564 12809-12815 NN denotes ligand
T5565 12815-12816 -RRB- denotes )
T5566 12817-12826 VBN denotes expressed
T5567 12827-12829 IN denotes by
T5568 12830-12833 CD denotes HI5
T5569 12834-12840 JJ denotes insect
T5570 12841-12846 NNS denotes cells
T5571 12847-12848 VBP denotes [
T5572 12848-12849 CD denotes 7
T5573 12849-12850 NNP denotes ]
T5574 12850-12851 , denotes ,
T5575 12852-12853 NNP denotes [
T5576 12853-12854 CD denotes 8
T5577 12854-12855 NNP denotes ]
T5578 12856-12859 CC denotes and
T5579 12860-12868 VBN denotes measured
T5580 12869-12879 NN denotes activation
T5581 12880-12882 IN denotes of
T5582 12883-12886 DT denotes the
T5583 12887-12892 NNP denotes NF-κB
T5584 12893-12896 CC denotes and
T5585 12897-12900 NNP denotes JNK
T5586 12901-12909 NNS denotes pathways
T5587 12909-12910 , denotes ,
T5588 12911-12914 CD denotes two
T5589 12915-12917 IN denotes of
T5590 12918-12921 DT denotes the
T5591 12922-12927 JJ denotes major
T5592 12928-12943 JJ denotes transcriptional
T5593 12944-12954 NNS denotes regulators
T5594 12955-12964 VBN denotes activated
T5595 12965-12967 IN denotes by
T5596 12968-12972 NNP denotes CD40
T5597 12973-12974 NNP denotes [
T5598 12974-12975 CD denotes 4
T5599 12975-12976 NNP denotes ]
T5600 12976-12977 . denotes .
T5601 12978-12993 JJ denotes Cell-associated
T5602 12994-12999 NNP denotes CD154
T5603 13000-13003 VBD denotes was
T5604 13004-13008 VBN denotes used
T5605 13009-13011 IN denotes as
T5606 13012-13015 DT denotes the
T5607 13016-13024 NN denotes stimulus
T5608 13025-13027 IN denotes in
T5609 13028-13033 DT denotes these
T5610 13034-13045 NNS denotes experiments
T5611 13046-13048 IN denotes as
T5612 13049-13051 PRP denotes it
T5613 13052-13061 RB denotes typically
T5614 13062-13070 VBZ denotes provides
T5615 13071-13075 JJR denotes more
T5616 13076-13082 JJ denotes robust
T5617 13082-13083 , denotes ,
T5618 13084-13087 CC denotes and
T5619 13088-13097 RB denotes therefore
T5620 13098-13102 RBR denotes more
T5621 13103-13110 RB denotes readily
T5622 13111-13119 VBN denotes detected
T5623 13119-13120 , denotes ,
T5624 13121-13131 NN denotes activation
T5625 13132-13139 NNS denotes signals
T5626 13140-13144 IN denotes than
T5627 13145-13149 VBZ denotes does
T5628 13150-13159 JJ denotes anti-CD40
T5629 13160-13168 NN denotes antibody
T5630 13168-13169 . denotes .
T5631 13170-13180 NNP denotes Activation
T5632 13181-13183 IN denotes of
T5633 13184-13187 DT denotes the
T5634 13188-13197 JJ denotes canonical
T5635 13198-13203 NN denotes NF-κB
T5636 13204-13211 NN denotes pathway
T5637 13212-13214 VBZ denotes is
T5638 13215-13224 VBN denotes initiated
T5639 13225-13229 IN denotes with
T5640 13230-13233 DT denotes the
T5641 13234-13249 NN denotes phosphorylation
T5642 13250-13252 IN denotes of
T5643 13253-13256 NNP denotes IκB
T5644 13257-13265 NNS denotes proteins
T5645 13266-13268 IN denotes by
T5646 13269-13272 DT denotes the
T5647 13273-13276 NNP denotes IκB
T5648 13277-13283 NN denotes kinase
T5649 13284-13291 NN denotes complex
T5650 13292-13293 -LRB- denotes (
T5651 13293-13296 NNP denotes IKK
T5652 13296-13297 -RRB- denotes )
T5653 13297-13298 . denotes .
T5654 13299-13301 IN denotes In
T5655 13302-13309 VBG denotes resting
T5656 13310-13315 NNS denotes cells
T5657 13315-13316 , denotes ,
T5658 13317-13320 NNP denotes IκB
T5659 13321-13329 NNS denotes proteins
T5660 13330-13333 VBP denotes are
T5661 13334-13345 JJ denotes responsible
T5662 13346-13349 IN denotes for
T5663 13350-13362 NN denotes sequestering
T5664 13363-13368 NN denotes NF-κB
T5665 13369-13377 NNS denotes subunits
T5666 13378-13380 IN denotes in
T5667 13381-13384 DT denotes the
T5668 13385-13394 NN denotes cytoplasm
T5669 13394-13395 . denotes .
T5670 13396-13411 NN denotes Phosphorylation
T5671 13412-13414 IN denotes by
T5672 13415-13418 DT denotes the
T5673 13419-13422 NNP denotes IKK
T5674 13423-13430 JJ denotes complex
T6738 13423-13430 NN denotes complex
T5675 13431-13438 NNS denotes targets
T5676 13439-13442 VBP denotes IκB
T5677 13443-13451 NNS denotes proteins
T5678 13452-13455 IN denotes for
T5679 13456-13470 NN denotes ubiquitination
T5680 13471-13474 CC denotes and
T5681 13475-13486 NN denotes degradation
T5682 13486-13487 , denotes ,
T5683 13488-13496 VBG denotes allowing
T5684 13497-13502 NN denotes NF-κB
T5685 13503-13505 TO denotes to
T5686 13506-13511 VB denotes enter
T5687 13512-13515 DT denotes the
T5688 13516-13523 NN denotes nucleus
T5689 13524-13527 CC denotes and
T5690 13528-13536 VB denotes activate
T5691 13537-13541 NN denotes gene
T5692 13542-13552 NN denotes expression
T5693 13552-13553 . denotes .
T5694 13554-13567 JJ denotes CD40-mediated
T5695 13568-13583 NN denotes phosphorylation
T5696 13584-13587 CC denotes and
T5697 13588-13599 NN denotes degradation
T5698 13600-13602 IN denotes of
T5699 13603-13607 NNP denotes IκBα
T5700 13608-13610 IN denotes in
T5701 13611-13625 JJ denotes HOIP-deficient
T5702 13626-13631 NNS denotes cells
T5703 13632-13635 VBD denotes was
T5704 13636-13648 RB denotes dramatically
T5705 13649-13657 VBN denotes impaired
T5706 13658-13666 JJ denotes relative
T5707 13667-13669 TO denotes to
T5708 13670-13674 DT denotes that
T5709 13675-13683 VBN denotes observed
T5710 13684-13686 IN denotes in
T5711 13687-13695 JJ denotes parental
T5712 13696-13699 NNP denotes A20
T5713 13699-13701 CD denotes .2
T5714 13701-13702 NNP denotes J
T5715 13703-13708 NNS denotes cells
T5716 13709-13710 -LRB- denotes (
T5717 13710-13714 JJ denotes Fig.
T5718 13715-13716 CD denotes 4
T5719 13716-13717 -RRB- denotes )
T5720 13717-13718 . denotes .
T5721 13719-13721 PRP denotes We
T5722 13722-13726 RB denotes also
T5723 13727-13734 VBD denotes assayed
T5724 13735-13745 NN denotes activation
T5725 13746-13748 IN denotes of
T5726 13749-13752 DT denotes the
T5727 13753-13769 JJ denotes stress-activated
T5728 13770-13777 NN denotes protein
T5729 13778-13784 NN denotes kinase
T5730 13785-13788 NNP denotes JNK
T5731 13789-13791 IN denotes in
T5732 13792-13800 NN denotes response
T5733 13801-13803 TO denotes to
T5734 13804-13808 CD denotes CD40
T5735 13809-13819 NN denotes engagement
T5736 13820-13821 -LRB- denotes (
T5737 13821-13825 FW denotes Fig.
T5738 13826-13827 LS denotes 4
T5739 13827-13828 -RRB- denotes )
T5740 13828-13829 . denotes .
T5741 13830-13843 JJ denotes CD40-mediated
T5742 13844-13847 NNP denotes JNK
T5743 13848-13858 NN denotes activation
T5744 13859-13861 IN denotes in
T5745 13862-13876 JJ denotes HOIP-deficient
T5746 13877-13882 NNS denotes cells
T5747 13883-13886 VBD denotes was
T5748 13887-13895 VBN denotes impaired
T5749 13896-13898 IN denotes as
T5750 13899-13907 VBN denotes measured
T5751 13908-13910 IN denotes by
T5752 13911-13926 NN denotes phosphorylation
T5753 13927-13929 IN denotes of
T5754 13930-13936 NNP denotes Thr183
T5755 13937-13940 CC denotes and
T5756 13941-13947 NNP denotes Tyr185
T5757 13948-13950 IN denotes in
T5758 13951-13954 NNP denotes JNK
T5759 13954-13955 . denotes .
T5760 13956-13968 JJ denotes CD40-induced
T5761 13969-13979 NN denotes activation
T5762 13980-13982 IN denotes of
T5763 13983-13988 NNP denotes NF-κB
T5764 13989-13992 CC denotes and
T5765 13993-13996 NNP denotes JNK
T5766 13997-13999 IN denotes in
T5767 14000-14018 JJ denotes HOIP-reconstituted
T5768 14019-14024 NNS denotes cells
T5769 14025-14028 VBD denotes was
T5770 14029-14035 JJ denotes normal
T5771 14035-14036 , denotes ,
T5772 14037-14050 VBG denotes demonstrating
T5773 14051-14055 IN denotes that
T5774 14056-14059 DT denotes the
T5775 14060-14067 NNS denotes defects
T5776 14068-14076 VBD denotes observed
T5777 14077-14079 IN denotes in
T5778 14080-14094 JJ denotes gene-deficient
T5779 14095-14100 NNS denotes cells
T5780 14101-14105 VBD denotes were
T5781 14106-14109 JJ denotes due
T5782 14110-14112 TO denotes to
T5783 14113-14116 DT denotes the
T5784 14117-14124 NN denotes absence
T5785 14125-14127 IN denotes of
T5786 14128-14132 NNP denotes HOIP
T5787 14133-14143 NN denotes expression
T5788 14143-14144 . denotes .
T16192 14189-14201 JJ denotes CD40-induced
T16193 14202-14212 NN denotes activation
T16194 14213-14215 IN denotes of
T16195 14216-14221 NNP denotes NF-κB
T16196 14222-14225 CC denotes and
T16197 14226-14229 NNP denotes JNK
T16198 14230-14232 VBZ denotes is
T16199 14233-14242 JJ denotes defective
T16200 14243-14245 IN denotes in
T16201 14246-14260 JJ denotes HOIP-deficient
T16202 14261-14266 NNS denotes cells
T16203 14266-14267 . denotes .
T16204 14268-14281 JJ denotes CD40-mediated
T16205 14282-14291 VBG denotes signaling
T16206 14292-14295 VBD denotes was
T16207 14296-14305 JJ denotes defective
T16208 14306-14308 IN denotes in
T16209 14309-14323 JJ denotes HOIP-deficient
T16210 14324-14329 NNS denotes cells
T16211 14330-14331 -LRB- denotes (
T16212 14331-14335 SYM denotes HOIP
T16213 14335-14336 : denotes -
T16214 14336-14337 SYM denotes /
T16215 14337-14338 : denotes -
T16216 14339-14340 NN denotes +
T16217 14341-14345 NNP denotes pMIP
T16218 14345-14346 -RRB- denotes )
T16219 14346-14347 , denotes ,
T16220 14348-14351 CC denotes but
T16221 14352-14358 JJ denotes intact
T16222 14359-14361 IN denotes in
T16223 14362-14370 JJ denotes parental
T16224 14371-14376 NNS denotes cells
T16225 14377-14387 VBN denotes transduced
T16226 14388-14392 IN denotes with
T16227 14393-14395 DT denotes an
T16228 14396-14401 JJ denotes empty
T16229 14402-14412 NN denotes retroviral
T16230 14413-14419 NN denotes vector
T16231 14420-14421 -LRB- denotes (
T16232 14421-14424 CD denotes A20
T16233 14424-14426 CD denotes .2
T16234 14426-14427 NNP denotes J
T16235 14428-14429 CD denotes +
T16236 14430-14434 NNP denotes pMIP
T16237 14434-14435 -RRB- denotes )
T16238 14436-14439 CC denotes and
T16239 14440-14442 IN denotes in
T16240 14443-14457 JJ denotes HOIP-deficient
T16241 14458-14463 NNS denotes cells
T16242 14464-14474 VBN denotes transduced
T16243 14475-14479 IN denotes with
T16244 14480-14481 DT denotes a
T16245 14482-14492 JJ denotes retroviral
T16246 14493-14499 NN denotes vector
T16247 14500-14508 VBG denotes encoding
T16248 14509-14513 NNP denotes HOIP
T16249 14514-14515 -LRB- denotes (
T16250 14515-14519 NNP denotes HOIP
T16251 14519-14520 : denotes -
T16252 14520-14521 SYM denotes /
T16253 14521-14522 : denotes -
T16254 14523-14524 NN denotes +
T16255 14525-14529 NNP denotes HOIP
T16256 14529-14530 -RRB- denotes )
T16257 14530-14531 . denotes .
T16258 14532-14537 NNS denotes Cells
T16259 14538-14542 VBD denotes were
T16260 14543-14552 VBN denotes activated
T16261 14553-14557 IN denotes with
T16262 14558-14574 JJ denotes CD154-expressing
T16263 14575-14581 NN denotes insect
T16264 14582-14587 NNS denotes cells
T16265 14588-14591 IN denotes for
T16266 14592-14595 DT denotes the
T16267 14596-14601 NNS denotes times
T16268 14602-14611 VBD denotes indicated
T16269 14611-14612 . denotes .
T16270 14613-14615 IN denotes As
T16271 14616-14617 DT denotes a
T16272 14618-14626 JJ denotes negative
T16273 14627-14634 NN denotes control
T16274 14634-14635 , denotes ,
T16275 14636-14641 NNS denotes cells
T16276 14642-14646 VBD denotes were
T16277 14647-14657 VBN denotes stimulated
T16278 14658-14662 IN denotes with
T16279 14663-14669 JJ denotes insect
T16280 14670-14675 NNS denotes cells
T16281 14676-14683 VBG denotes lacking
T16282 14684-14689 NNP denotes CD154
T16283 14690-14691 -LRB- denotes (
T16284 14691-14692 CD denotes 5
T16285 14693-14699 JJ denotes minute
T16286 14700-14704 NN denotes time
T16287 14705-14710 NN denotes point
T16288 14711-14715 RB denotes only
T16289 14715-14716 -RRB- denotes )
T16290 14716-14717 . denotes .
T16291 14718-14725 JJ denotes Western
T16292 14726-14731 NNS denotes blots
T16293 14732-14734 IN denotes of
T16294 14735-14745 JJ denotes whole-cell
T16295 14746-14753 NNS denotes lysates
T16296 14754-14758 VBD denotes were
T16297 14759-14765 VBN denotes probed
T16298 14766-14770 IN denotes with
T16299 14771-14774 DT denotes the
T16300 14775-14784 VBN denotes indicated
T16301 14785-14795 NNS denotes antibodies
T16302 14795-14796 . denotes .
T16303 14797-14809 NNP denotes Phospho-IκBα
T16304 14810-14811 -LRB- denotes (
T16305 14811-14816 NNP denotes pIκBα
T16306 14816-14817 -RRB- denotes )
T16307 14818-14821 CC denotes and
T16308 14822-14833 NNP denotes phospho-JNK
T16309 14834-14835 -LRB- denotes (
T16310 14835-14839 NNP denotes pJNK
T16311 14839-14840 -RRB- denotes )
T16312 14841-14846 NNS denotes blots
T16313 14847-14851 VBD denotes were
T16314 14852-14860 VBN denotes stripped
T16315 14861-14864 CC denotes and
T16316 14865-14873 VBN denotes reprobed
T16317 14874-14877 IN denotes for
T16318 14878-14883 JJ denotes total
T16319 14884-14888 NNP denotes IκBα
T16320 14889-14892 CC denotes and
T16321 14893-14898 JJ denotes total
T16322 14899-14902 NNP denotes JNK
T16323 14902-14903 , denotes ,
T16324 14904-14916 RB denotes respectively
T16325 14917-14918 -LRB- denotes (
T16326 14918-14926 JJ denotes anti-JNK
T16327 14927-14937 NNS denotes antibodies
T16328 14938-14947 VBP denotes recognize
T16329 14948-14951 CD denotes p46
T16330 14952-14955 CC denotes and
T16331 14956-14959 CD denotes p54
T16332 14960-14968 NNS denotes isoforms
T16333 14968-14969 -RRB- denotes )
T16334 14969-14970 . denotes .
T16335 14971-14975 NNP denotes IκBα
T16336 14976-14981 NNS denotes blots
T16337 14982-14986 VBD denotes were
T16338 14987-14991 RB denotes also
T16339 14992-15000 VBN denotes reprobed
T16340 15001-15004 IN denotes for
T16341 15005-15010 NN denotes actin
T16342 15011-15013 TO denotes to
T16343 15014-15025 VB denotes demonstrate
T16344 15026-15031 JJ denotes equal
T16345 15032-15036 NN denotes lane
T16346 15037-15044 VBG denotes loading
T16347 15044-15045 . denotes .
T16348 15046-15055 JJ denotes Molecular
T16349 15056-15063 NNS denotes weights
T16350 15064-15067 VBP denotes are
T16351 15068-15077 VBN denotes indicated
T16352 15078-15080 IN denotes at
T16353 15081-15086 NN denotes right
T16354 15086-15087 . denotes .
T16355 15088-15095 JJ denotes Similar
T16356 15096-15103 NNS denotes results
T16357 15104-15108 VBD denotes were
T16358 15109-15117 VBN denotes obtained
T16359 15118-15120 IN denotes in
T16360 15121-15122 DT denotes a
T16361 15123-15129 JJ denotes second
T16362 15130-15140 NN denotes experiment
T16363 15141-15144 CC denotes and
T16364 15145-15147 IN denotes in
T16365 15148-15151 CD denotes two
T16366 15152-15162 JJ denotes additional
T16367 15163-15174 NNS denotes experiments
T16368 15175-15179 IN denotes with
T16369 15180-15181 DT denotes a
T16370 15182-15188 JJ denotes second
T16371 15189-15203 JJ denotes HOIP-deficient
T16372 15204-15209 NN denotes clone
T16373 15209-15210 . denotes .
T6739 15265-15269 IN denotes with
T6740 15270-15274 NNP denotes CD40
T6741 15275-15278 NNP denotes The
T6742 15279-15285 VBD denotes marked
T6743 15286-15293 NNS denotes defects
T6744 15294-15296 IN denotes in
T6745 15297-15310 JJ denotes CD40-mediated
T6746 15311-15315 NN denotes cell
T6747 15316-15326 NN denotes activation
T6748 15327-15330 CC denotes and
T6749 15331-15340 VBG denotes signaling
T6750 15341-15350 VBN denotes displayed
T6751 15351-15353 IN denotes by
T6752 15354-15368 JJ denotes HOIP-deficient
T6753 15369-15374 NNS denotes cells
T6754 15375-15384 VBD denotes suggested
T6755 15385-15389 IN denotes that
T6756 15390-15394 NNP denotes HOIP
T6757 15395-15403 VBZ denotes mediates
T6758 15404-15415 NN denotes recruitment
T6759 15416-15418 IN denotes of
T6760 15419-15427 JJ denotes critical
T6761 15428-15438 NNS denotes components
T6762 15439-15441 IN denotes of
T6763 15442-15445 DT denotes the
T6764 15446-15450 NNP denotes CD40
T6765 15451-15460 VBG denotes signaling
T6766 15461-15470 NN denotes apparatus
T6767 15471-15473 TO denotes to
T6768 15474-15477 DT denotes the
T6769 15478-15486 NN denotes receptor
T6770 15486-15487 . denotes .
T6771 15488-15498 RB denotes Previously
T6772 15498-15499 , denotes ,
T6773 15500-15502 PRP denotes we
T6774 15503-15515 VBD denotes demonstrated
T6775 15516-15520 IN denotes that
T6776 15521-15525 NNP denotes HOIP
T6777 15526-15528 VBZ denotes is
T6778 15529-15538 VBN denotes recruited
T6779 15539-15541 TO denotes to
T6780 15542-15545 DT denotes the
T6781 15546-15550 NNP denotes CD40
T6782 15551-15560 VBG denotes signaling
T6783 15561-15568 JJ denotes complex
T6784 15569-15571 IN denotes in
T6785 15572-15573 DT denotes a
T6786 15574-15589 JJ denotes TRAF2-dependent
T6787 15590-15596 NN denotes manner
T6788 15596-15597 , denotes ,
T6789 15598-15608 VBG denotes suggesting
T6790 15609-15613 IN denotes that
T6791 15614-15618 NNP denotes HOIP
T6792 15619-15628 NNS denotes functions
T6793 15629-15639 JJ denotes downstream
T6794 15640-15642 IN denotes of
T6795 15643-15648 CD denotes TRAF2
T6796 15649-15650 NN denotes [
T6797 15650-15651 CD denotes 6
T6798 15651-15652 NNP denotes ]
T6799 15652-15653 . denotes .
T6800 15654-15661 NNS denotes Studies
T6801 15662-15664 IN denotes by
T6802 15665-15671 NNS denotes others
T6803 15672-15679 VBP denotes suggest
T6804 15680-15684 IN denotes that
T6805 15685-15688 DT denotes the
T6806 15689-15705 JJ denotes TRAF2-associated
T6807 15706-15714 NNS denotes proteins
T6808 15715-15720 JJ denotes cIAP1
T6809 15721-15724 CC denotes and
T6810 15725-15730 JJ denotes cIAP2
T6811 15731-15735 NN denotes play
T6812 15736-15737 DT denotes a
T6813 15738-15742 NN denotes role
T6814 15743-15745 IN denotes in
T6815 15746-15749 DT denotes the
T6816 15750-15761 NN denotes recruitment
T6817 15762-15764 IN denotes of
T6818 15765-15769 NNP denotes HOIP
T6819 15770-15772 TO denotes to
T6820 15773-15778 CD denotes TNFR1
T6821 15779-15782 CC denotes and
T6822 15783-15787 CD denotes CD40
T6823 15788-15789 NNP denotes [
T6824 15789-15791 CD denotes 14
T6825 15791-15792 NNP denotes ]
T6826 15792-15793 . denotes .
T6827 15794-15803 RB denotes Therefore
T6828 15803-15804 , denotes ,
T6829 15805-15807 PRP denotes we
T6830 15808-15818 VBD denotes determined
T6831 15819-15826 IN denotes whether
T6832 15827-15830 DT denotes the
T6833 15831-15842 NN denotes association
T6834 15843-15845 IN denotes of
T6835 15846-15850 NNP denotes HOIP
T6836 15851-15855 IN denotes with
T6837 15856-15860 NNP denotes CD40
T6838 15861-15863 IN denotes in
T6839 15864-15867 NNP denotes A20
T6840 15867-15869 CD denotes .2
T6841 15869-15870 NNP denotes J
T6842 15871-15876 NNS denotes cells
T6843 15877-15880 VBD denotes was
T6844 15881-15888 VBN denotes altered
T6845 15889-15891 IN denotes by
T6846 15892-15901 NN denotes treatment
T6847 15902-15904 IN denotes of
T6848 15905-15910 NNS denotes cells
T6849 15911-15915 IN denotes with
T6850 15916-15918 DT denotes an
T6851 15919-15928 NN denotes inhibitor
T6852 15929-15931 IN denotes of
T6853 15932-15936 JJ denotes cIAP
T6854 15937-15945 NN denotes activity
T6855 15945-15946 , denotes ,
T6856 15947-15948 DT denotes a
T6857 15949-15967 JJ denotes membrane-permeable
T6858 15968-15975 NN denotes peptide
T6859 15976-15983 VBN denotes derived
T6860 15984-15988 IN denotes from
T6861 15989-15992 DT denotes the
T6862 15993-16002 NN denotes apoptosis
T6863 16003-16012 NN denotes regulator
T6864 16013-16017 NNP denotes SMAC
T6865 16018-16019 NNP denotes [
T6866 16019-16021 CD denotes 15
T6867 16021-16022 NNP denotes ]
T6868 16022-16023 . denotes .
T6869 16024-16026 PRP denotes We
T6870 16027-16032 VBD denotes found
T6871 16033-16037 IN denotes that
T6872 16038-16050 NN denotes pretreatment
T6873 16051-16053 IN denotes of
T6874 16054-16059 NNS denotes cells
T6875 16060-16064 IN denotes with
T6876 16065-16068 DT denotes the
T6877 16069-16073 NNP denotes SMAC
T6878 16074-16081 NN denotes peptide
T6879 16082-16094 RB denotes dramatically
T6880 16095-16102 VBD denotes reduced
T6881 16103-16106 DT denotes the
T6882 16107-16113 NN denotes amount
T6883 16114-16116 IN denotes of
T6884 16117-16122 JJ denotes cIAP1
T6885 16123-16133 VBN denotes associated
T6886 16134-16138 IN denotes with
T6887 16139-16142 DT denotes the
T6888 16143-16147 NNP denotes CD40
T6889 16148-16157 VBG denotes signaling
T6890 16158-16165 JJ denotes complex
T6891 16166-16168 IN denotes in
T6892 16169-16174 NNS denotes cells
T6893 16175-16185 VBN denotes stimulated
T6894 16186-16190 IN denotes with
T6895 16191-16200 JJ denotes anti-CD40
T6896 16201-16216 JJ denotes antibody-coated
T6897 16217-16222 NNS denotes beads
T6898 16223-16224 -LRB- denotes (
T6899 16224-16228 NNP denotes Fig.
T6900 16229-16231 NNP denotes 5A
T6901 16231-16232 -RRB- denotes )
T6902 16232-16233 . denotes .
T6903 16234-16238 NNP denotes SMAC
T6904 16239-16246 NN denotes peptide
T6905 16247-16256 NN denotes treatment
T6906 16257-16261 RB denotes also
T6907 16262-16270 VBD denotes resulted
T6908 16271-16273 IN denotes in
T6909 16274-16275 DT denotes a
T6910 16276-16282 JJ denotes slight
T6911 16283-16286 CC denotes but
T6912 16287-16299 JJ denotes reproducible
T6913 16300-16308 NN denotes decrease
T6914 16309-16311 IN denotes in
T6915 16312-16315 DT denotes the
T6916 16316-16322 NN denotes amount
T6917 16323-16325 IN denotes of
T6918 16326-16329 DT denotes the
T6919 16330-16335 JJ denotes major
T6920 16336-16340 NNP denotes HOIP
T6921 16341-16345 NN denotes form
T6922 16346-16355 VBD denotes recovered
T6923 16356-16358 IN denotes by
T6924 16359-16363 NNP denotes CD40
T6925 16364-16383 NN denotes immunoprecipitation
T6926 16383-16384 , denotes ,
T6927 16385-16390 IN denotes along
T6928 16391-16395 IN denotes with
T6929 16396-16398 DT denotes an
T6930 16399-16407 JJ denotes apparent
T6931 16408-16416 NN denotes increase
T6932 16417-16419 IN denotes in
T6933 16420-16426 JJR denotes higher
T6934 16427-16436 JJ denotes molecular
T6935 16437-16443 NN denotes weight
T6936 16444-16451 NNS denotes species
T6937 16452-16462 VBN denotes recognized
T6938 16463-16465 IN denotes by
T6939 16466-16475 JJ denotes anti-HOIP
T6940 16476-16484 NN denotes antibody
T6941 16484-16485 . denotes .
T6942 16486-16488 IN denotes In
T6943 16489-16497 NN denotes contrast
T6944 16497-16498 , denotes ,
T6945 16499-16508 NN denotes treatment
T6946 16509-16513 IN denotes with
T6947 16514-16517 DT denotes the
T6948 16518-16522 NNP denotes SMAC
T6949 16523-16530 NN denotes peptide
T6950 16531-16534 VBD denotes did
T6951 16535-16538 RB denotes not
T6952 16539-16544 VB denotes alter
T6953 16545-16548 DT denotes the
T6954 16549-16555 NN denotes amount
T6955 16556-16558 CC denotes or
T6956 16559-16568 JJ denotes molecular
T6957 16569-16575 NN denotes weight
T6958 16576-16578 IN denotes of
T6959 16579-16583 NNP denotes HOIP
T6960 16584-16591 NN denotes present
T6961 16592-16594 IN denotes in
T6962 16595-16599 NN denotes cell
T6963 16600-16607 NNS denotes lysates
T6964 16607-16608 . denotes .
T6965 16609-16614 DT denotes These
T6966 16615-16619 NNS denotes data
T6967 16620-16627 VBP denotes suggest
T6968 16628-16632 IN denotes that
T6969 16633-16637 NNP denotes SMAC
T6970 16638-16645 NN denotes peptide
T6971 16646-16655 NN denotes treatment
T6972 16656-16668 RB denotes specifically
T6973 16669-16675 VBZ denotes alters
T6974 16676-16679 DT denotes the
T6975 16680-16695 NNS denotes characteristics
T6976 16696-16698 IN denotes of
T6977 16699-16714 JJ denotes CD40-associated
T6978 16715-16719 NNP denotes HOIP
T6979 16720-16726 RB denotes rather
T6980 16727-16731 IN denotes than
T6981 16732-16735 DT denotes the
T6982 16736-16742 JJ denotes entire
T6983 16743-16751 JJ denotes cellular
T6984 16752-16756 NN denotes pool
T6985 16757-16759 IN denotes of
T6986 16760-16764 DT denotes this
T6987 16765-16772 NN denotes protein
T6988 16772-16773 . denotes .
T6989 16774-16782 RB denotes Together
T6990 16782-16783 , denotes ,
T6991 16784-16789 DT denotes these
T6992 16790-16797 NNS denotes results
T6993 16798-16805 VBP denotes support
T6994 16806-16809 DT denotes the
T6995 16810-16814 NN denotes idea
T6996 16815-16819 IN denotes that
T6997 16820-16823 DT denotes the
T6998 16824-16828 NNP denotes cIAP
T6999 16829-16837 NNS denotes proteins
T7000 16838-16847 VBP denotes influence
T7001 16848-16851 DT denotes the
T7002 16852-16863 NN denotes recruitment
T7003 16864-16867 CC denotes and
T7004 16868-16886 JJ denotes post-translational
T7005 16887-16899 NN denotes modification
T7006 16900-16905 NN denotes state
T7007 16906-16908 IN denotes of
T7008 16909-16924 JJ denotes CD40-associated
T7009 16925-16929 NNP denotes HOIP
T7010 16929-16930 . denotes .
T16830 16975-16978 NNP denotes IKK
T16831 16979-16990 NN denotes recruitment
T16832 16991-16993 TO denotes to
T16833 16994-16997 DT denotes the
T16834 16998-17002 NNP denotes CD40
T16835 17003-17012 VBG denotes signaling
T16836 17013-17020 NN denotes complex
T16837 17021-17023 VBZ denotes is
T16838 17024-17033 JJ denotes defective
T16839 17034-17036 IN denotes in
T16840 17037-17051 JJ denotes HOIP-deficient
T16841 17052-17057 NNS denotes cells
T16842 17057-17058 . denotes .
T16843 17059-17060 -LRB- denotes (
T16844 17060-17061 NNP denotes A
T16845 17061-17062 -RRB- denotes )
T16846 17063-17067 NNP denotes SMAC
T16847 17068-17075 NN denotes peptide
T16848 17076-17085 NN denotes treatment
T16849 17086-17093 VBZ denotes reduces
T16850 17094-17105 NN denotes recruitment
T16851 17106-17108 IN denotes of
T16852 17109-17114 NNP denotes cIAP1
T16853 17115-17117 TO denotes to
T16854 17118-17122 NNP denotes CD40
T16855 17123-17126 CC denotes and
T16856 17127-17130 MD denotes may
T16857 17131-17137 VB denotes modify
T16858 17138-17142 NNP denotes HOIP
T16859 17143-17154 NN denotes recruitment
T16860 17154-17155 . denotes .
T16861 17156-17159 CD denotes A20
T16862 17159-17161 CD denotes .2
T16863 17161-17162 NNP denotes J
T16864 17163-17168 NNS denotes cells
T16865 17169-17173 VBD denotes were
T16866 17174-17183 VBN denotes incubated
T16867 17184-17187 IN denotes for
T16868 17188-17191 CD denotes six
T16869 17192-17197 NNS denotes hours
T16870 17198-17202 IN denotes with
T16871 17203-17221 JJ denotes membrane-permeable
T16872 17222-17229 NNP denotes SMAC-N7
T16873 17230-17237 NN denotes peptide
T16874 17238-17240 CC denotes or
T16875 17241-17244 CD denotes 1.5
T16876 17244-17245 NN denotes %
T16877 17246-17250 NNP denotes DMSO
T16878 17251-17252 -LRB- denotes (
T16879 17252-17259 JJ denotes solvent
T16880 17260-17264 VBN denotes used
T16881 17265-17268 IN denotes for
T16882 17269-17272 DT denotes the
T16883 17273-17280 NN denotes peptide
T16884 17280-17281 -RRB- denotes )
T16885 17281-17282 . denotes .
T16886 17283-17292 VBG denotes Following
T16887 17293-17296 DT denotes the
T16888 17297-17307 NN denotes incubation
T16889 17307-17308 , denotes ,
T16890 17309-17313 NN denotes cell
T16891 17314-17321 NNS denotes lysates
T16892 17322-17326 VBD denotes were
T16893 17327-17335 VBN denotes prepared
T16894 17335-17336 , denotes ,
T16895 17337-17349 VBN denotes fractionated
T16896 17350-17352 IN denotes by
T16897 17353-17361 NNP denotes SDS-PAGE
T16898 17361-17362 , denotes ,
T16899 17363-17366 CC denotes and
T16900 17367-17376 VBN denotes evaluated
T16901 17377-17379 IN denotes by
T16902 17380-17387 JJ denotes Western
T16903 17388-17392 NN denotes blot
T16904 17393-17394 -LRB- denotes (
T16905 17394-17399 NNS denotes lanes
T16906 17400-17401 CD denotes 1
T16907 17402-17405 CC denotes and
T16908 17406-17407 CD denotes 2
T16909 17407-17408 -RRB- denotes )
T16910 17408-17409 . denotes .
T16911 17410-17415 NNS denotes Cells
T16912 17416-17425 VBD denotes incubated
T16913 17426-17430 IN denotes with
T16914 17431-17435 NNP denotes DMSO
T16915 17436-17438 CC denotes or
T16916 17439-17446 NNP denotes SMAC-N7
T16917 17447-17451 VBD denotes were
T16918 17452-17456 RB denotes also
T16919 17457-17467 VBN denotes stimulated
T16920 17468-17472 IN denotes with
T16921 17473-17481 JJ denotes magnetic
T16922 17482-17487 NNS denotes beads
T16923 17488-17494 VBN denotes coated
T16924 17495-17499 IN denotes with
T16925 17500-17509 JJ denotes anti-CD40
T16927 17510-17512 CC denotes or
T16928 17513-17515 DT denotes an
T16929 17516-17523 NN denotes isotype
T16930 17524-17531 NN denotes control
T16931 17532-17540 NN denotes antibody
T16932 17540-17541 . denotes .
T16933 17542-17560 VBN denotes Immunoprecipitated
T16934 17561-17562 -LRB- denotes (
T16935 17562-17564 NNP denotes IP
T16936 17564-17565 -RRB- denotes )
T16937 17566-17574 NN denotes material
T16938 17575-17580 VBN denotes bound
T16939 17581-17583 TO denotes to
T16940 17584-17587 DT denotes the
T16941 17588-17593 NNS denotes beads
T16942 17594-17597 VBD denotes was
T16943 17598-17604 VBN denotes loaded
T16944 17605-17607 IN denotes in
T16945 17608-17613 NNS denotes lanes
T16946 17614-17617 CD denotes 3-5
T16947 17617-17618 . denotes .
T16948 17619-17626 NNS denotes Samples
T16949 17627-17629 IN denotes of
T16950 17630-17633 DT denotes the
T16951 17634-17638 NN denotes cell
T16952 17639-17646 VBZ denotes lysates
T16953 17647-17652 IN denotes after
T16954 17653-17672 NN denotes immunoprecipitation
T16955 17673-17679 VBP denotes appear
T16956 17680-17682 IN denotes in
T16957 17683-17688 NNS denotes lanes
T16958 17689-17690 CD denotes 6
T16959 17691-17692 CD denotes 8
T16960 17692-17693 . denotes .
T16961 17694-17701 JJ denotes Western
T16962 17702-17707 NNS denotes blots
T16963 17708-17712 VBD denotes were
T16964 17713-17719 VBN denotes probed
T16965 17720-17724 IN denotes with
T16966 17725-17735 NNS denotes antibodies
T16967 17736-17744 JJ denotes specific
T16968 17745-17748 IN denotes for
T16969 17749-17754 NNP denotes cIAP1
T16970 17754-17755 , denotes ,
T16971 17756-17761 CD denotes TRAF2
T16972 17761-17762 , denotes ,
T16973 17763-17768 CD denotes TRAF3
T16974 17768-17769 , denotes ,
T16975 17770-17773 CC denotes and
T16976 17774-17778 NNP denotes HOIP
T16977 17779-17780 -LRB- denotes (
T16978 17780-17791 JJ denotes approximate
T16979 17792-17801 JJ denotes molecular
T16980 17802-17809 NNS denotes weights
T16981 17810-17819 VBD denotes indicated
T16982 17820-17822 IN denotes on
T16983 17823-17827 NN denotes left
T16984 17827-17828 -RRB- denotes )
T16985 17828-17829 . denotes .
T16986 17830-17837 JJ denotes Similar
T16987 17838-17845 NNS denotes results
T16988 17846-17850 VBD denotes were
T16989 17851-17859 VBN denotes obtained
T16990 17860-17862 IN denotes in
T16991 17863-17866 CD denotes two
T16992 17867-17877 JJ denotes additional
T16993 17878-17889 NNS denotes experiments
T16994 17889-17890 . denotes .
T16995 17891-17892 -LRB- denotes (
T16996 17892-17893 NNP denotes B
T16997 17893-17894 -RRB- denotes )
T16998 17895-17899 NNP denotes CD40
T16999 17900-17903 VBD denotes was
T17000 17904-17912 VBN denotes isolated
T17001 17913-17915 IN denotes by
T17002 17916-17935 NN denotes immunoprecipitation
T17003 17936-17937 -LRB- denotes (
T17004 17937-17939 IN denotes as
T17005 17940-17942 IN denotes in
T17006 17943-17944 -LRB- denotes (
T17007 17944-17945 NN denotes A
T17008 17945-17946 -RRB- denotes )
T17009 17946-17947 -RRB- denotes )
T17010 17948-17952 IN denotes from
T17011 17953-17956 CD denotes A20
T17012 17956-17958 CD denotes .2
T17013 17958-17959 NNP denotes J
T17014 17960-17965 NNS denotes cells
T17015 17966-17969 CC denotes and
T17016 17970-17984 JJ denotes HOIP-deficient
T17017 17985-17990 NNS denotes cells
T17018 17991-18001 VBN denotes transduced
T16926 18002-18006 IN denotes with
T17019 18007-18009 DT denotes an
T17020 18010-18015 JJ denotes empty
T17021 18016-18026 NN denotes retroviral
T17022 18027-18033 NN denotes vector
T17023 18034-18035 -LRB- denotes (
T17024 18035-18039 NNP denotes pMIP
T17025 18039-18040 -RRB- denotes )
T17026 18041-18043 CC denotes or
T17027 18044-18045 DT denotes a
T17028 18046-18056 JJ denotes retroviral
T17029 18057-18063 NN denotes vector
T17030 18064-18072 VBG denotes encoding
T17031 18073-18077 NNP denotes HOIP
T17032 18077-18078 . denotes .
T17033 18079-18087 NNP denotes Material
T17034 18088-18106 VBD denotes immunoprecipitated
T17035 18107-18111 IN denotes with
T17036 18112-18114 DT denotes an
T17037 18115-18122 NN denotes isotype
T17038 18123-18130 NN denotes control
T17039 18131-18139 NN denotes antibody
T17040 18140-18141 -LRB- denotes (
T17041 18141-18148 NN denotes isotype
T17042 18148-18149 -RRB- denotes )
T17043 18150-18152 CC denotes or
T17044 18153-18162 JJ denotes anti-CD40
T17045 18163-18171 NN denotes antibody
T17046 18172-18175 VBD denotes was
T17047 18176-18184 VBN denotes examined
T17048 18185-18187 IN denotes by
T17049 18188-18195 JJ denotes Western
T17050 18196-18204 VBG denotes blotting
T17051 18205-18208 IN denotes for
T17052 18209-18213 NNP denotes CD40
T17053 18213-18214 , denotes ,
T17054 18215-18220 CD denotes TRAF2
T17055 18220-18221 , denotes ,
T17056 18222-18227 CD denotes TRAF3
T17057 18227-18228 , denotes ,
T17058 18229-18234 NNP denotes cIAP1
T17059 18234-18235 , denotes ,
T17060 18236-18240 NNP denotes HOIP
T17061 18240-18241 , denotes ,
T17062 18242-18246 NNP denotes IKKα
T17063 18246-18247 NNP denotes /
T17064 18247-18248 NN denotes β
T17065 18248-18249 , denotes ,
T17066 18250-18253 CC denotes and
T17067 18254-18258 NNP denotes IKKγ
T17068 18259-18260 -LRB- denotes (
T17069 18260-18265 JJ denotes right
T17070 18266-18272 NNS denotes panels
T17071 18272-18273 -RRB- denotes )
T17072 18273-18274 . denotes .
T17073 18275-18279 NNP denotes HOIP
T17074 18280-18290 NN denotes expression
T17075 18291-18294 VBD denotes was
T17076 18295-18303 VBN denotes required
T17077 18304-18307 IN denotes for
T17078 18308-18323 NN denotes coprecipitation
T17079 18324-18326 IN denotes of
T17080 18327-18330 NNP denotes IKK
T17081 18331-18339 NNS denotes proteins
T17082 18340-18344 IN denotes with
T17083 18345-18349 NNP denotes CD40
T17084 18349-18350 . denotes .
T17085 18351-18355 NNP denotes Cell
T17086 18356-18363 VBZ denotes lysates
T17087 18364-18368 IN denotes from
T17088 18369-18381 JJ denotes unstimulated
T17089 18382-18387 NNS denotes cells
T17090 18388-18391 VBP denotes are
T17091 18392-18397 VBN denotes shown
T17092 18398-18400 IN denotes in
T17093 18401-18404 DT denotes the
T17094 18405-18409 VBN denotes left
T17095 18410-18416 NNS denotes panels
T17096 18416-18417 . denotes .
T17097 18418-18425 JJ denotes Similar
T17098 18426-18433 NNS denotes results
T17099 18434-18438 VBD denotes were
T17100 18439-18447 VBN denotes obtained
T17101 18448-18450 IN denotes in
T17102 18451-18452 DT denotes a
T17103 18453-18459 JJ denotes second
T17104 18460-18470 NN denotes experiment
T17105 18471-18474 CC denotes and
T17106 18475-18477 IN denotes in
T17107 18478-18481 CD denotes two
T17108 18482-18493 NNS denotes experiments
T17109 18494-18498 IN denotes with
T17110 18499-18500 DT denotes a
T17111 18501-18507 JJ denotes second
T17112 18508-18522 JJ denotes HOIP-deficient
T17113 18523-18528 NN denotes clone
T17114 18528-18529 . denotes .
T17115 18530-18531 -LRB- denotes (
T17116 18531-18532 $ denotes C
T17117 18532-18533 -RRB- denotes )
T17119 18534-18536 TO denotes To
T17120 18537-18544 RBR denotes further
T17121 18545-18553 VB denotes evaluate
T17122 18554-18568 JJ denotes HOIP-dependent
T17123 18569-18580 NN denotes recruitment
T17124 18581-18583 IN denotes of
T17125 18584-18588 NNP denotes IKKγ
T17126 18589-18591 TO denotes to
T17127 18592-18596 NNP denotes CD40
T17128 18596-18597 , denotes ,
T17129 18598-18601 NNP denotes A20
T17130 18601-18603 CD denotes .2
T17131 18603-18604 NNP denotes J
T17132 18605-18610 NNS denotes cells
T17133 18611-18613 CC denotes or
T17134 18614-18628 JJ denotes HOIP-deficient
T17135 18629-18630 -LRB- denotes (
T17136 18630-18634 SYM denotes HOIP
T17137 18634-18635 : denotes -
T17138 18635-18636 SYM denotes /
T17139 18636-18637 : denotes -
T17140 18637-18638 -RRB- denotes )
T17141 18639-18642 CD denotes A20
T17142 18642-18644 CD denotes .2
T17143 18644-18645 NNP denotes J
T17144 18646-18651 NNS denotes cells
T17145 18652-18656 VBD denotes were
T17146 18657-18667 VBN denotes transduced
T17147 18668-18672 IN denotes with
T17148 18673-18675 DT denotes an
T17149 18676-18681 JJ denotes empty
T17150 18682-18692 NN denotes retroviral
T17151 18693-18699 NN denotes vector
T17152 18700-18702 CC denotes or
T17153 18703-18704 DT denotes a
T17154 18705-18715 JJ denotes retroviral
T17155 18716-18725 VBP denotes construct
T17156 18726-18734 VBG denotes encoding
T17157 18735-18737 NNP denotes BP
T17158 18738-18752 JJ denotes epitope-tagged
T17159 18753-18757 NNP denotes IKKγ
T17160 18758-18759 -LRB- denotes (
T17161 18759-18764 VBN denotes noted
T17162 18765-18767 IN denotes in
T17163 18768-18771 DT denotes the
T17164 18772-18778 NN denotes figure
T17165 18779-18781 IN denotes as
T17166 18782-18786 NNP denotes pMIP
T17167 18787-18790 CC denotes and
T17168 18791-18795 NNP denotes IKKγ
T17169 18795-18796 , denotes ,
T17170 18797-18809 RB denotes respectively
T17171 18809-18810 -RRB- denotes )
T17172 18810-18811 . denotes .
T17173 18812-18819 NNP denotes Lysates
T17174 18820-18821 -LRB- denotes (
T17175 18821-18826 NNS denotes lanes
T17176 18827-18828 CD denotes 1
T17177 18829-18830 LS denotes 3
T17178 18830-18831 -RRB- denotes )
T17179 18832-18835 CC denotes and
T17180 18836-18855 NN denotes immunoprecipitation
T17181 18856-18857 -LRB- denotes (
T17182 18857-18859 NNP denotes IP
T17183 18859-18860 -RRB- denotes )
T17184 18861-18868 NNS denotes samples
T17185 18869-18870 -LRB- denotes (
T17186 18870-18875 NNS denotes lanes
T17187 18876-18877 CD denotes 4
T17188 18878-18880 CD denotes 11
T17189 18880-18881 -RRB- denotes )
T17190 18882-18886 IN denotes from
T17191 18887-18890 DT denotes the
T17192 18891-18895 NN denotes cell
T17193 18896-18901 NNS denotes lines
T17194 18902-18906 VBD denotes were
T17195 18907-18919 VBN denotes fractionated
T17196 18920-18922 IN denotes by
T17197 18923-18931 NNP denotes SDS-PAGE
T17198 18932-18935 CC denotes and
T17199 18936-18945 VBN denotes evaluated
T17200 18946-18948 IN denotes by
T17201 18949-18956 JJ denotes Western
T17118 18957-18965 VBG denotes blotting
T17202 18966-18970 IN denotes with
T17203 18971-18981 NNS denotes antibodies
T17204 18982-18984 TO denotes to
T17205 18985-18988 DT denotes the
T17206 18989-18991 NNP denotes BP
T17207 18992-18995 NN denotes tag
T17208 18996-18997 -LRB- denotes (
T17209 18997-19001 NNP denotes IKKγ
T17210 19001-19002 , denotes ,
T17211 19003-19008 JJ denotes upper
T17212 19009-19014 NN denotes panel
T17213 19014-19015 -RRB- denotes )
T17214 19016-19019 CC denotes and
T17215 19020-19025 CD denotes TRAF2
T17216 19025-19026 . denotes .
T17217 19027-19030 DT denotes The
T17218 19031-19040 JJ denotes anti-CD40
T17219 19041-19043 NNP denotes IP
T17220 19044-19050 NN denotes sample
T17221 19051-19053 IN denotes in
T17222 19054-19058 NN denotes lane
T17223 19059-19061 CD denotes 11
T17224 19062-19065 VBD denotes was
T17225 19066-19073 VBN denotes treated
T17226 19074-19078 IN denotes with
T17227 19079-19080 JJ denotes λ
T17228 19081-19092 NN denotes phosphatase
T17229 19092-19093 : denotes ;
T17230 19094-19097 DT denotes the
T17231 19098-19104 NN denotes sample
T17232 19105-19107 IN denotes in
T17233 19108-19112 NN denotes lane
T17234 19113-19115 CD denotes 10
T17235 19116-19119 VBD denotes was
T17236 19120-19132 JJ denotes mock-treated
T17237 19132-19133 . denotes .
T17238 19134-19141 NN denotes Protein
T17239 19142-19149 NNS denotes samples
T17240 19150-19151 -LRB- denotes (
T17241 19151-19156 CC denotes minus
T17242 19157-19162 DT denotes those
T17243 19163-19170 VBN denotes treated
T17244 19171-19175 IN denotes with
T17245 19176-19187 NN denotes phosphatase
T17246 19187-19188 -RRB- denotes )
T17247 19189-19193 VBD denotes were
T17248 19194-19198 RB denotes also
T17249 19199-19211 VBN denotes fractionated
T17250 19212-19214 IN denotes on
T17251 19215-19216 DT denotes a
T17252 19217-19225 JJ denotes separate
T17253 19226-19229 NN denotes gel
T17254 19230-19231 -LRB- denotes (
T17255 19231-19236 JJR denotes lower
T17256 19237-19247 NN denotes acrylamide
T17257 19248-19261 NN denotes concentration
T17258 19261-19262 -RRB- denotes )
T17259 19263-19266 IN denotes for
T17260 19267-19270 DT denotes the
T17261 19271-19281 NN denotes evaluation
T17262 19282-19284 IN denotes of
T17263 19285-19290 CD denotes TRAF3
T17264 19291-19294 CC denotes and
T17265 19295-19299 NNP denotes HOIP
T17266 19300-19301 -LRB- denotes (
T17267 19301-19307 JJ denotes bottom
T17268 19308-19311 CD denotes two
T17269 19312-19318 NNS denotes panels
T17270 19318-19319 -RRB- denotes )
T17271 19319-19320 . denotes .
T17272 19321-19328 JJ denotes Similar
T17273 19329-19336 NNS denotes results
T17274 19337-19341 VBD denotes were
T17275 19342-19350 VBN denotes obtained
T17276 19351-19353 IN denotes in
T17277 19354-19357 CD denotes two
T17278 19358-19368 JJ denotes additional
T17279 19369-19380 NNS denotes experiments
T17280 19380-19381 . denotes .
T7011 19384-19386 TO denotes To
T7012 19387-19391 VB denotes test
T7013 19392-19395 DT denotes the
T7014 19396-19407 NN denotes possibility
T7015 19408-19412 WDT denotes that
T7016 19413-19417 NNP denotes HOIP
T7017 19418-19420 VBZ denotes is
T7018 19421-19432 JJ denotes responsible
T7019 19433-19436 IN denotes for
T7020 19437-19440 DT denotes the
T7021 19441-19452 NN denotes recruitment
T7022 19453-19455 IN denotes of
T7023 19456-19461 JJ denotes other
T7024 19462-19470 JJ denotes critical
T7025 19471-19480 VBG denotes signaling
T7026 19481-19489 NNS denotes proteins
T7027 19490-19492 TO denotes to
T7028 19493-19497 NNP denotes CD40
T7029 19497-19498 , denotes ,
T7030 19499-19501 PRP denotes we
T7031 19502-19520 VBD denotes immunoprecipitated
T7032 19521-19525 NNP denotes CD40
T7033 19526-19535 VBG denotes signaling
T7034 19536-19545 NNS denotes complexes
T7035 19546-19550 IN denotes from
T7036 19551-19565 JJ denotes HOIP-deficient
T7037 19566-19569 CC denotes and
T7038 19570-19588 JJ denotes HOIP-reconstituted
T7039 19589-19594 NNS denotes cells
T7040 19595-19596 -LRB- denotes (
T7041 19596-19600 NNP denotes Fig.
T7042 19601-19603 NNP denotes 5B
T7043 19603-19604 -RRB- denotes )
T7044 19604-19605 . denotes .
T7045 19606-19608 IN denotes As
T7046 19609-19618 VBN denotes described
T7047 19619-19629 RB denotes previously
T7048 19629-19630 , denotes ,
T7049 19631-19635 NNP denotes CD40
T7050 19636-19645 VBG denotes signaling
T7051 19646-19655 NNS denotes complexes
T7052 19656-19674 VBN denotes immunoprecipitated
T7053 19675-19679 IN denotes from
T7054 19680-19683 CD denotes A20
T7055 19683-19685 CD denotes .2
T7056 19685-19686 NNP denotes J
T7057 19687-19692 NNS denotes cells
T7058 19693-19702 VBD denotes contained
T7059 19703-19707 NNP denotes HOIP
T7060 19708-19710 RB denotes as
T7061 19711-19715 RB denotes well
T7062 19716-19718 IN denotes as
T7063 19719-19724 CD denotes TRAF2
T7064 19724-19725 , denotes ,
T7065 19726-19731 CD denotes TRAF3
T7066 19731-19732 , denotes ,
T7067 19733-19737 NNP denotes IKKα
T7068 19737-19738 NNP denotes /
T7069 19738-19739 NN denotes β
T7070 19739-19740 , denotes ,
T7071 19741-19744 CC denotes and
T7072 19745-19749 NNP denotes IKKγ
T7073 19750-19751 NNP denotes [
T7074 19751-19752 CD denotes 6
T7075 19752-19753 NNP denotes ]
T7076 19753-19754 . denotes .
T7077 19755-19758 DT denotes The
T7078 19759-19766 NNS denotes amounts
T7079 19767-19769 IN denotes of
T7080 19770-19775 CD denotes TRAF2
T7081 19775-19776 , denotes ,
T7082 19777-19782 CD denotes TRAF3
T7083 19782-19783 , denotes ,
T7084 19784-19787 CC denotes and
T7085 19788-19793 NNP denotes cIAP1
T7086 19794-19796 IN denotes in
T7087 19797-19801 NNP denotes CD40
T7088 19802-19820 VBZ denotes immunoprecipitates
T7089 19821-19825 IN denotes from
T7090 19826-19840 JJ denotes HOIP-deficient
T7091 19841-19846 NNS denotes cells
T7092 19847-19851 VBD denotes were
T7093 19852-19859 JJ denotes similar
T7094 19860-19862 TO denotes to
T7095 19863-19868 DT denotes those
T7096 19869-19873 IN denotes from
T7097 19874-19882 JJ denotes parental
T7098 19883-19886 NNP denotes A20
T7099 19886-19888 CD denotes .2
T7100 19888-19889 NNP denotes J
T7101 19890-19895 NNS denotes cells
T7102 19895-19896 , denotes ,
T7103 19897-19907 VBG denotes indicating
T7104 19908-19912 IN denotes that
T7105 19913-19917 NNP denotes HOIP
T7106 19918-19920 VBZ denotes is
T7107 19921-19924 RB denotes not
T7108 19925-19933 VBN denotes required
T7109 19934-19937 IN denotes for
T7110 19938-19941 DT denotes the
T7111 19942-19953 NN denotes association
T7112 19954-19956 IN denotes of
T7113 19957-19962 DT denotes these
T7114 19963-19971 NNS denotes proteins
T7115 19972-19976 IN denotes with
T7116 19977-19981 NNP denotes CD40
T7117 19981-19982 . denotes .
T7118 19983-19985 IN denotes In
T7119 19986-19994 NN denotes contrast
T7120 19994-19995 , denotes ,
T7121 19996-20000 NNP denotes IKKα
T7122 20000-20001 NNP denotes /
T7123 20001-20002 NN denotes β
T7124 20003-20006 CC denotes and
T7125 20007-20011 NNP denotes IKKγ
T7126 20012-20016 VBD denotes were
T7127 20017-20020 RB denotes not
T7128 20021-20031 JJ denotes detectable
T7129 20032-20034 IN denotes in
T7130 20035-20039 CD denotes CD40
T7131 20040-20058 NNS denotes immunoprecipitates
T7132 20059-20068 VBD denotes recovered
T7133 20069-20073 IN denotes from
T7134 20074-20088 JJ denotes HOIP-deficient
T7135 20089-20094 NNS denotes cells
T7136 20095-20096 -LRB- denotes (
T7137 20096-20100 NNP denotes Fig.
T7138 20101-20103 NNP denotes 5B
T7139 20103-20104 , denotes ,
T7140 20104-20105 NNP denotes C
T7141 20105-20106 -RRB- denotes )
T7142 20106-20107 . denotes .
T7143 20108-20111 DT denotes The
T7144 20112-20119 NNS denotes amounts
T7145 20120-20122 IN denotes of
T7146 20123-20127 NNP denotes IKKα
T7147 20127-20128 NNP denotes /
T7148 20128-20129 NN denotes β
T7149 20130-20133 CC denotes and
T7150 20134-20138 NNP denotes IKKγ
T7151 20139-20141 IN denotes in
T7152 20142-20146 NNP denotes CD40
T7153 20147-20165 VBZ denotes immunoprecipitates
T7154 20166-20170 IN denotes from
T7155 20171-20189 JJ denotes HOIP-reconstituted
T7156 20190-20195 NNS denotes cells
T7157 20196-20200 VBD denotes were
T7158 20201-20208 JJ denotes similar
T7159 20209-20211 TO denotes to
T7160 20212-20217 DT denotes those
T7161 20218-20226 VBN denotes detected
T7162 20227-20229 IN denotes in
T7163 20230-20237 NNS denotes samples
T7164 20238-20246 VBN denotes prepared
T7165 20247-20251 IN denotes from
T7166 20252-20260 JJ denotes parental
T7167 20261-20266 NNS denotes cells
T7168 20266-20267 , denotes ,
T7169 20268-20281 VBG denotes demonstrating
T7170 20282-20286 IN denotes that
T7171 20287-20290 DT denotes the
T7172 20291-20298 NNS denotes defects
T7173 20299-20301 IN denotes in
T7174 20302-20305 NNP denotes IKK
T7175 20306-20317 NN denotes recruitment
T7176 20318-20320 PRP denotes we
T7177 20321-20329 VBD denotes observed
T7178 20330-20334 VBD denotes were
T7179 20335-20338 JJ denotes due
T7180 20339-20351 RB denotes specifically
T7181 20352-20354 TO denotes to
T7182 20355-20358 DT denotes the
T7183 20359-20366 NN denotes absence
T7184 20367-20369 IN denotes of
T7185 20370-20374 NNP denotes HOIP
T7186 20375-20385 NN denotes expression
T7187 20385-20386 . denotes .
T7188 20387-20392 DT denotes These
T7189 20393-20397 NNS denotes data
T7190 20398-20406 VBP denotes indicate
T7191 20407-20411 IN denotes that
T7192 20412-20416 NNP denotes HOIP
T7193 20417-20419 VBZ denotes is
T7194 20420-20428 VBN denotes required
T7195 20429-20432 IN denotes for
T7196 20433-20444 NN denotes recruitment
T7197 20445-20447 IN denotes of
T7198 20448-20451 DT denotes the
T7199 20452-20468 JJ denotes NF-κB-activating
T7200 20469-20476 NN denotes complex
T7201 20477-20479 TO denotes to
T7202 20480-20483 DT denotes the
T7203 20484-20488 NNP denotes CD40
T7204 20489-20498 VBG denotes signaling
T7205 20499-20506 NN denotes complex
T7206 20506-20507 . denotes .
T7207 20508-20510 IN denotes As
T7208 20511-20516 VBN denotes shown
T7209 20517-20521 RB denotes here
T7210 20522-20525 CC denotes and
T7211 20526-20528 IN denotes in
T7212 20529-20532 PRP$ denotes our
T7213 20533-20541 JJ denotes previous
T7214 20542-20547 NN denotes study
T7215 20548-20549 NN denotes [
T7216 20549-20550 CD denotes 6
T7217 20550-20551 NNP denotes ]
T7218 20551-20552 , denotes ,
T7219 20553-20557 NNP denotes IKKγ
T7220 20558-20565 NN denotes present
T7221 20566-20568 IN denotes in
T7222 20569-20573 NNP denotes CD40
T7223 20574-20592 VBZ denotes immunoprecipitates
T7224 20593-20600 VBZ denotes appears
T7225 20601-20603 TO denotes to
T7226 20604-20608 VB denotes have
T7227 20609-20610 DT denotes a
T7228 20611-20617 JJR denotes higher
T7229 20618-20627 JJ denotes molecular
T7230 20628-20634 NN denotes weight
T7231 20635-20639 IN denotes than
T7232 20640-20644 DT denotes that
T7233 20645-20650 VBN denotes found
T7234 20651-20653 IN denotes in
T7235 20654-20658 NN denotes cell
T7236 20659-20666 NNS denotes lysates
T7237 20666-20667 . denotes .
T7238 20668-20670 TO denotes To
T7239 20671-20678 VB denotes confirm
T7240 20679-20683 IN denotes that
T7241 20684-20688 DT denotes this
T7242 20689-20695 JJR denotes higher
T7243 20696-20705 JJ denotes molecular
T7244 20706-20712 NN denotes weight
T7245 20713-20720 NNS denotes species
T7246 20721-20724 VBD denotes was
T7247 20725-20731 RB denotes indeed
T7248 20732-20736 NNP denotes IKKγ
T7249 20736-20737 , denotes ,
T7250 20738-20740 PRP denotes we
T7251 20741-20750 VBD denotes generated
T7252 20751-20754 NNP denotes A20
T7253 20754-20756 CD denotes .2
T7254 20756-20757 NNP denotes J
T7255 20758-20762 NN denotes cell
T7256 20763-20768 NNS denotes lines
T7257 20769-20779 VBG denotes containing
T7258 20780-20781 DT denotes a
T7259 20782-20788 RB denotes stably
T7260 20789-20799 VBN denotes integrated
T7261 20800-20810 JJ denotes retroviral
T7262 20811-20817 NN denotes vector
T7263 20818-20822 WDT denotes that
T7264 20823-20830 VBD denotes encoded
T7265 20831-20833 DT denotes an
T7266 20834-20848 JJ denotes epitope-tagged
T7267 20849-20856 NN denotes version
T7268 20857-20859 IN denotes of
T7269 20860-20865 NN denotes mouse
T7270 20866-20870 NNP denotes IKKγ
T7271 20870-20871 . denotes .
T7272 20872-20876 NN denotes Cell
T7273 20877-20884 NNS denotes lysates
T7274 20885-20888 CC denotes and
T7275 20889-20893 JJ denotes CD40
T7276 20894-20912 NNS denotes immunoprecipitates
T7277 20913-20921 VBN denotes prepared
T7278 20922-20926 IN denotes from
T7279 20927-20932 DT denotes these
T7280 20933-20938 NNS denotes cells
T7281 20939-20943 VBD denotes were
T7282 20944-20952 VBN denotes analyzed
T7283 20953-20955 IN denotes by
T7284 20956-20963 JJ denotes Western
T7285 20964-20972 VBG denotes blotting
T7286 20973-20976 IN denotes for
T7287 20977-20980 DT denotes the
T7288 20981-20988 NN denotes epitope
T7289 20989-20992 NN denotes tag
T7290 20993-20994 -LRB- denotes (
T7291 20994-20998 NNP denotes Fig.
T7292 20999-21001 NNP denotes 5C
T7293 21001-21002 -RRB- denotes )
T7294 21002-21003 . denotes .
T7295 21004-21008 DT denotes This
T7296 21009-21017 NN denotes analysis
T7297 21018-21026 VBD denotes produced
T7298 21027-21028 DT denotes a
T7299 21029-21036 NN denotes pattern
T7300 21037-21039 IN denotes of
T7301 21040-21045 NNS denotes bands
T7302 21046-21050 WDT denotes that
T7303 21051-21054 VBD denotes was
T7304 21055-21066 RB denotes essentially
T7305 21067-21070 DT denotes the
T7306 21071-21075 JJ denotes same
T7307 21076-21078 IN denotes as
T7308 21079-21083 DT denotes that
T7309 21084-21092 VBN denotes obtained
T7310 21093-21098 VBG denotes using
T7311 21099-21101 DT denotes an
T7312 21102-21110 NN denotes antibody
T7313 21111-21119 NN denotes specific
T7314 21120-21123 IN denotes for
T7315 21124-21130 JJ denotes native
T7316 21131-21135 NNP denotes IKKγ
T7317 21136-21137 -LRB- denotes (
T7318 21137-21144 VB denotes compare
T7319 21145-21151 NNS denotes panels
T7320 21152-21153 NNP denotes B
T7321 21154-21157 CC denotes and
T7322 21158-21159 NNP denotes C
T7323 21160-21162 IN denotes in
T7324 21163-21167 NNP denotes Fig.
T7325 21168-21169 CD denotes 5
T7326 21169-21170 -RRB- denotes )
T7327 21170-21171 . denotes .
T7328 21172-21180 RB denotes Moreover
T7329 21180-21181 , denotes ,
T7330 21182-21190 NN denotes analysis
T7331 21191-21193 IN denotes of
T7332 21194-21199 NNS denotes cells
T7333 21200-21210 VBG denotes expressing
T7334 21211-21218 NN denotes epitope
T7335 21219-21230 NN denotes tagged-IKKγ
T7336 21231-21240 VBD denotes confirmed
T7337 21241-21245 IN denotes that
T7338 21246-21257 NN denotes recruitment
T7339 21258-21260 IN denotes of
T7340 21261-21265 NNP denotes IKKγ
T7341 21266-21268 TO denotes to
T7342 21269-21273 NNP denotes CD40
T7343 21274-21282 VBZ denotes requires
T7344 21283-21287 NNP denotes HOIP
T7345 21287-21288 . denotes .
T7346 21289-21291 TO denotes To
T7347 21292-21301 VB denotes determine
T7348 21302-21306 WP denotes what
T7349 21307-21310 VBD denotes was
T7350 21311-21322 JJ denotes responsible
T7351 21323-21326 IN denotes for
T7352 21327-21330 DT denotes the
T7353 21331-21340 VBN denotes increased
T7354 21341-21350 JJ denotes molecular
T7355 21351-21357 NN denotes weight
T7356 21358-21360 IN denotes of
T7357 21361-21365 NNP denotes IKKγ
T7358 21366-21368 IN denotes in
T7359 21369-21373 NNP denotes CD40
T7360 21374-21392 NNS denotes immunoprecipitates
T7361 21392-21393 , denotes ,
T7362 21394-21397 DT denotes the
T7363 21398-21405 NN denotes protein
T7364 21406-21413 NNS denotes samples
T7365 21414-21418 VBD denotes were
T7366 21419-21428 VBN denotes incubated
T7367 21429-21433 IN denotes with
T7368 21434-21440 JJ denotes lambda
T7369 21441-21452 NN denotes phosphatase
T7370 21452-21453 , denotes ,
T7371 21454-21459 WDT denotes which
T7372 21460-21467 VBZ denotes removes
T7373 21468-21478 NNS denotes phosphates
T7374 21479-21487 VBN denotes attached
T7375 21488-21490 TO denotes to
T7376 21491-21499 VB denotes tyrosine
T7377 21499-21500 , denotes ,
T7378 21501-21510 NN denotes threonine
T7379 21510-21511 , denotes ,
T7380 21512-21514 CC denotes or
T7381 21515-21521 NN denotes serine
T7382 21522-21530 NNS denotes residues
T7383 21531-21533 IN denotes in
T7384 21534-21542 NNS denotes proteins
T7385 21542-21543 . denotes .
T7386 21544-21548 DT denotes This
T7387 21549-21558 NN denotes treatment
T7388 21559-21566 VBN denotes reduced
T7389 21567-21571 RB denotes much
T7390 21572-21574 IN denotes of
T7391 21575-21590 JJ denotes CD40-associated
T7392 21591-21595 NNP denotes IKKγ
T7393 21596-21598 TO denotes to
T7394 21599-21601 DT denotes an
T7395 21602-21610 JJ denotes apparent
T7396 21611-21620 JJ denotes molecular
T7397 21621-21627 NN denotes weight
T7398 21628-21635 JJ denotes similar
T7399 21636-21638 TO denotes to
T7400 21639-21643 DT denotes that
T7401 21644-21646 IN denotes of
T7402 21647-21650 DT denotes the
T7403 21651-21658 NN denotes protein
T7404 21659-21661 IN denotes in
T7405 21662-21666 NN denotes cell
T7406 21667-21674 NNS denotes lysates
T7407 21675-21676 -LRB- denotes (
T7408 21676-21680 NNP denotes Fig.
T7409 21681-21683 NNP denotes 5C
T7410 21683-21684 -RRB- denotes )
T7411 21684-21685 . denotes .
T7412 21686-21693 RB denotes However
T7413 21693-21694 , denotes ,
T7414 21695-21697 IN denotes at
T7415 21698-21703 JJS denotes least
T7416 21704-21707 CD denotes one
T7417 21708-21712 NN denotes band
T7418 21713-21715 IN denotes of
T7419 21716-21729 RB denotes significantly
T7420 21730-21736 JJR denotes higher
T7421 21737-21746 JJ denotes molecular
T7422 21747-21753 NN denotes weight
T7423 21754-21762 VBD denotes remained
T7424 21763-21768 IN denotes after
T7425 21769-21780 NN denotes phosphatase
T7426 21781-21790 NN denotes treatment
T7427 21790-21791 , denotes ,
T7428 21792-21802 VBG denotes suggesting
T7429 21803-21807 IN denotes that
T7430 21808-21823 JJ denotes CD40-associated
T7431 21824-21828 NNP denotes IKKγ
T7432 21829-21831 VBZ denotes is
T7433 21832-21839 JJ denotes subject
T7434 21840-21842 TO denotes to
T7435 21843-21845 IN denotes at
T7436 21846-21851 JJS denotes least
T7437 21852-21855 CD denotes one
T7438 21856-21861 JJ denotes other
T7439 21862-21874 NN denotes modification
T7440 21875-21877 IN denotes in
T7441 21878-21886 NN denotes addition
T7442 21887-21889 TO denotes to
T7443 21890-21905 NN denotes phosphorylation
T7444 21905-21906 . denotes .
T7445 21907-21914 RB denotes Overall
T7446 21914-21915 , denotes ,
T7447 21916-21921 DT denotes these
T7448 21922-21926 NNS denotes data
T7449 21927-21938 VBP denotes demonstrate
T7450 21939-21943 IN denotes that
T7451 21944-21948 NNP denotes HOIP
T7452 21949-21951 VBZ denotes is
T7453 21952-21960 VBN denotes required
T7454 21961-21964 IN denotes for
T7455 21965-21968 DT denotes the
T7456 21969-21980 NN denotes association
T7457 21981-21983 IN denotes of
T7458 21984-21988 NNP denotes IKKγ
T7459 21989-21993 IN denotes with
T7460 21994-21998 NNP denotes CD40
T7461 21998-21999 , denotes ,
T7462 22000-22003 CC denotes and
T7463 22004-22011 VBP denotes suggest
T7464 22012-22016 IN denotes that
T7465 22017-22021 DT denotes this
T7466 22022-22027 NN denotes event
T7467 22028-22030 VBZ denotes is
T7468 22031-22038 VBN denotes coupled
T7469 22039-22041 TO denotes to
T7470 22042-22060 JJ denotes post-translational
T7471 22061-22074 NNS denotes modifications
T7472 22075-22077 IN denotes of
T7473 22078-22082 NNP denotes IKKγ
T7474 22082-22083 . denotes .
T9222 22096-22099 PRP$ denotes Our
T9223 22100-22107 NNS denotes results
T9224 22108-22116 VBP denotes indicate
T9225 22117-22121 IN denotes that
T9226 22122-22125 DT denotes the
T9227 22126-22133 NN denotes protein
T9228 22134-22138 NNP denotes HOIP
T9229 22139-22141 VBZ denotes is
T9230 22142-22150 JJ denotes critical
T9231 22151-22154 IN denotes for
T9232 22155-22167 JJ denotes CD40-induced
T9233 22168-22175 NNS denotes signals
T9234 22176-22180 WDT denotes that
T9235 22181-22189 VBP denotes regulate
T9236 22190-22191 NNP denotes B
T9237 22192-22196 NN denotes cell
T9238 22197-22205 NN denotes function
T9239 22205-22206 . denotes .
T9240 22207-22210 PRP$ denotes Our
T9241 22211-22215 NNS denotes data
T9242 22216-22220 VBP denotes show
T9243 22221-22225 IN denotes that
T9244 22226-22240 JJ denotes HOIP-dependent
T9245 22241-22249 JJ denotes cellular
T9246 22250-22259 NNS denotes responses
T9247 22260-22267 VBP denotes include
T9248 22268-22281 JJ denotes CD40-mediated
T9249 22282-22294 NN denotes upregulation
T9250 22295-22297 IN denotes of
T9251 22298-22302 CD denotes CD80
T9252 22303-22313 NN denotes expression
T9253 22314-22317 CC denotes and
T9254 22318-22327 NN denotes synthesis
T9255 22328-22330 IN denotes of
T9256 22331-22339 NN denotes germline
T9257 22340-22343 NNP denotes RNA
T9258 22344-22355 NNS denotes transcripts
T9259 22356-22360 IN denotes from
T9260 22361-22364 DT denotes the
T9261 22365-22379 NN denotes immunoglobulin
T9262 22380-22385 JJ denotes heavy
T9263 22386-22391 NN denotes chain
T9264 22392-22397 NN denotes locus
T9265 22397-22398 , denotes ,
T9266 22399-22402 CD denotes two
T9267 22403-22409 NNS denotes events
T9268 22410-22414 WDT denotes that
T9269 22415-22418 VBP denotes are
T9270 22419-22428 JJ denotes important
T9271 22429-22432 IN denotes for
T9272 22433-22449 JJ denotes T-cell-dependent
T9273 22450-22467 JJ denotes antibody-mediated
T9274 22468-22474 JJ denotes immune
T9275 22475-22484 NNS denotes responses
T9276 22484-22485 . denotes .
T9277 22486-22488 IN denotes At
T9278 22489-22492 DT denotes the
T9279 22493-22502 JJ denotes molecular
T9280 22503-22508 NN denotes level
T9281 22508-22509 , denotes ,
T9282 22510-22513 PRP$ denotes our
T9283 22514-22518 NNS denotes data
T9284 22519-22527 VBP denotes indicate
T9285 22528-22532 IN denotes that
T9286 22533-22537 NNP denotes HOIP
T9287 22538-22547 NNS denotes functions
T9288 22548-22558 JJ denotes downstream
T9289 22559-22561 IN denotes of
T9290 22562-22567 CD denotes TRAF2
T9291 22568-22570 IN denotes in
T9292 22571-22574 DT denotes the
T9293 22575-22579 NNP denotes CD40
T9294 22580-22589 VBG denotes signaling
T9295 22590-22597 NN denotes pathway
T9296 22598-22601 CC denotes and
T9297 22602-22606 IN denotes that
T9298 22607-22611 NNP denotes HOIP
T9299 22612-22615 VBZ denotes has
T9300 22616-22617 DT denotes a
T9301 22618-22621 JJ denotes key
T9302 22622-22626 NN denotes role
T9303 22627-22629 IN denotes in
T9304 22630-22639 VBG denotes promoting
T9305 22640-22643 DT denotes the
T9306 22644-22655 NN denotes recruitment
T9307 22656-22658 IN denotes of
T9308 22659-22662 DT denotes the
T9309 22663-22666 NNP denotes IKK
T9310 22667-22674 NN denotes complex
T9311 22675-22677 TO denotes to
T9312 22678-22682 NNP denotes CD40
T9313 22682-22683 . denotes .
T9314 22684-22694 JJ denotes Consistent
T9315 22695-22699 IN denotes with
T9316 22700-22704 DT denotes this
T9317 22704-22705 , denotes ,
T9318 22706-22718 JJ denotes CD40-induced
T9319 22719-22729 NN denotes activation
T9320 22730-22732 IN denotes of
T9321 22733-22738 NNP denotes NF-κB
T9322 22739-22741 VBZ denotes is
T9323 22742-22751 JJ denotes dependent
T9324 22752-22754 IN denotes on
T9325 22755-22758 DT denotes the
T9326 22759-22767 NN denotes presence
T9327 22768-22770 IN denotes of
T9328 22771-22775 NNP denotes HOIP
T9329 22775-22776 . denotes .
T9330 22777-22779 IN denotes In
T9331 22780-22788 NN denotes addition
T9332 22788-22789 , denotes ,
T9333 22790-22793 PRP$ denotes our
T9334 22794-22798 NNS denotes data
T9335 22799-22803 VBP denotes show
T9336 22804-22808 IN denotes that
T9337 22809-22813 NNP denotes HOIP
T9338 22814-22825 VBZ denotes facilitates
T9339 22826-22829 DT denotes the
T9340 22830-22840 NN denotes activation
T9341 22841-22843 IN denotes of
T9342 22844-22847 NNP denotes JNK
T9343 22848-22850 IN denotes in
T9344 22851-22859 NN denotes response
T9345 22860-22862 TO denotes to
T9346 22863-22867 CD denotes CD40
T9347 22868-22878 NN denotes engagement
T9348 22878-22879 . denotes .
T9349 22880-22888 RB denotes Together
T9350 22888-22889 , denotes ,
T9351 22890-22893 PRP$ denotes our
T9352 22894-22902 NNS denotes findings
T9353 22903-22910 VBP denotes provide
T9354 22911-22918 NN denotes support
T9355 22919-22922 IN denotes for
T9356 22923-22926 DT denotes the
T9357 22927-22937 NN denotes conclusion
T9358 22938-22942 WDT denotes that
T9359 22943-22947 NNP denotes HOIP
T9360 22948-22950 VBZ denotes is
T9361 22951-22952 DT denotes a
T9362 22953-22956 JJ denotes key
T9363 22957-22966 NN denotes component
T9364 22967-22969 IN denotes of
T9365 22970-22973 DT denotes the
T9366 22974-22978 NNP denotes CD40
T9367 22979-22988 VBG denotes signaling
T9368 22989-22996 NN denotes pathway
T9369 22996-22997 . denotes .
T9370 22998-23003 VBN denotes Given
T9371 23004-23007 DT denotes the
T9372 23008-23018 NN denotes importance
T9373 23019-23021 IN denotes of
T9374 23022-23026 NNP denotes CD40
T9375 23027-23036 VBG denotes signaling
T9376 23037-23039 IN denotes in
T9377 23040-23044 DT denotes both
T9378 23045-23053 JJ denotes cellular
T9379 23054-23057 CC denotes and
T9380 23058-23065 JJ denotes humoral
T9381 23066-23072 JJ denotes immune
T9382 23073-23082 NNS denotes responses
T9383 23082-23083 , denotes ,
T9384 23084-23087 PRP$ denotes our
T9385 23088-23095 NNS denotes results
T9386 23096-23104 VBP denotes indicate
T9387 23105-23109 IN denotes that
T9388 23110-23114 NNP denotes HOIP
T9389 23115-23118 VBZ denotes has
T9390 23119-23120 DT denotes a
T9391 23121-23129 JJ denotes critical
T9392 23130-23134 NN denotes role
T9393 23135-23137 IN denotes in
T9394 23138-23141 DT denotes the
T9395 23142-23152 NN denotes regulation
T9396 23153-23155 IN denotes of
T9397 23156-23159 DT denotes the
T9398 23160-23166 JJ denotes immune
T9399 23167-23173 NN denotes system
T9400 23173-23174 . denotes .
T9401 23175-23178 DT denotes The
T9402 23179-23189 JJ denotes functional
T9403 23190-23200 NNS denotes properties
T9404 23201-23203 IN denotes of
T9405 23204-23208 NNP denotes HOIP
T9406 23209-23213 VBP denotes have
T9407 23214-23218 RB denotes only
T9408 23219-23223 VBN denotes been
T9409 23224-23233 RB denotes partially
T9410 23234-23247 VBN denotes characterized
T9411 23247-23248 . denotes .
T9412 23249-23256 JJ denotes Initial
T9413 23257-23264 NNS denotes studies
T9414 23265-23271 VBD denotes showed
T9415 23272-23276 IN denotes that
T9416 23277-23281 NNP denotes HOIP
T9417 23282-23285 CC denotes and
T9418 23286-23289 DT denotes the
T9419 23290-23297 JJ denotes related
T9420 23298-23305 NN denotes protein
T9421 23306-23312 NN denotes HOIL-1
T9422 23313-23316 VBP denotes are
T9423 23317-23327 NNS denotes components
T9424 23328-23330 IN denotes of
T9425 23331-23332 DT denotes a
T9426 23333-23338 JJ denotes large
T9427 23339-23340 -LRB- denotes (
T9428 23340-23341 FW denotes ∼
T9429 23341-23344 CD denotes 600
T9430 23345-23348 NN denotes kDa
T9431 23348-23349 -RRB- denotes )
T9432 23350-23357 NN denotes protein
T9433 23358-23365 JJ denotes complex
T9434 23366-23373 JJ denotes capable
T9435 23374-23376 IN denotes of
T9436 23377-23389 VBG denotes synthesizing
T9437 23390-23396 JJ denotes linear
T9438 23397-23410 NN denotes polyubiquitin
T9439 23411-23417 NNS denotes chains
T9440 23418-23419 VBP denotes [
T9441 23419-23420 CD denotes 5
T9442 23420-23421 NNP denotes ]
T9443 23421-23422 . denotes .
T9444 23423-23433 JJ denotes Subsequent
T9445 23434-23441 NNS denotes studies
T9446 23442-23448 VBD denotes showed
T9447 23449-23453 IN denotes that
T9448 23454-23455 DT denotes a
T9449 23456-23471 JJ denotes HOIP-containing
T9450 23472-23479 NN denotes complex
T9451 23480-23483 MD denotes can
T9452 23484-23492 VB denotes interact
T9453 23493-23497 IN denotes with
T9454 23498-23502 NNP denotes IKKγ
T9455 23503-23506 CC denotes and
T9456 23507-23517 VB denotes facilitate
T9457 23518-23528 NN denotes activation
T9458 23529-23531 IN denotes of
T9459 23532-23537 NNP denotes NF-κB
T9460 23538-23541 IN denotes via
T9461 23542-23545 DT denotes the
T9462 23546-23555 JJ denotes canonical
T9463 23556-23563 NN denotes pathway
T9464 23564-23565 NNP denotes [
T9465 23565-23567 CD denotes 16
T9466 23567-23568 NNP denotes ]
T9467 23568-23569 . denotes .
T9468 23570-23575 DT denotes These
T9469 23576-23580 NNS denotes data
T9470 23580-23581 , denotes ,
T9471 23582-23592 VBN denotes considered
T9472 23593-23601 RB denotes together
T9473 23602-23606 IN denotes with
T9474 23607-23611 JJ denotes ours
T9475 23611-23612 , denotes ,
T9476 23613-23620 VBP denotes suggest
T9477 23621-23625 IN denotes that
T9478 23626-23627 DT denotes a
T9479 23628-23643 JJ denotes HOIP-containing
T9480 23644-23651 JJ denotes complex
T9481 23652-23660 NNS denotes mediates
T9482 23661-23672 NN denotes recruitment
T9483 23673-23675 IN denotes of
T9484 23676-23680 NNP denotes IKKγ
T9485 23681-23683 TO denotes to
T9486 23684-23687 DT denotes the
T9487 23688-23692 NNP denotes CD40
T9488 23693-23702 VBG denotes signaling
T9489 23703-23710 NN denotes complex
T9490 23710-23711 . denotes .
T9491 23712-23714 IN denotes In
T9492 23715-23723 NN denotes addition
T9493 23723-23724 , denotes ,
T9494 23725-23740 JJ denotes CD40-associated
T9495 23741-23745 NNP denotes HOIP
T9496 23746-23751 MD denotes could
T9497 23752-23756 VB denotes play
T9498 23757-23758 DT denotes a
T9499 23759-23763 NN denotes role
T9500 23764-23766 IN denotes in
T9501 23767-23777 VBG denotes activating
T9502 23778-23782 NNP denotes IKKγ
T9503 23783-23788 IN denotes after
T9504 23789-23792 PRP$ denotes its
T9505 23793-23804 NN denotes recruitment
T9506 23805-23807 TO denotes to
T9507 23808-23811 DT denotes the
T9508 23812-23821 VBG denotes signaling
T9509 23822-23829 JJ denotes complex
T9510 23830-23831 NNP denotes [
T9511 23831-23833 CD denotes 16
T9512 23833-23834 NNP denotes ]
T9513 23834-23835 . denotes .
T9514 23836-23839 DT denotes The
T9515 23840-23846 JJR denotes higher
T9516 23847-23856 JJ denotes molecular
T9517 23857-23863 NN denotes weight
T9518 23864-23869 NNS denotes forms
T9519 23870-23872 IN denotes of
T9520 23873-23877 NNP denotes IKKγ
T9521 23878-23880 PRP denotes we
T9522 23881-23889 VBD denotes observed
T9523 23890-23892 IN denotes in
T9524 23893-23897 CD denotes CD40
T9525 23898-23916 NNS denotes immunoprecipitates
T9526 23917-23922 MD denotes would
T9527 23923-23925 VB denotes be
T9528 23926-23936 JJ denotes consistent
T9529 23937-23941 IN denotes with
T9530 23942-23945 DT denotes the
T9531 23946-23954 NN denotes presence
T9532 23955-23957 IN denotes of
T9533 23958-23978 JJ denotes post-transcriptional
T9534 23979-23992 NNS denotes modifications
T9535 23993-24002 VBG denotes including
T9536 24003-24018 NN denotes phosphorylation
T9537 24019-24022 CC denotes and
T9538 24023-24037 NN denotes ubiquitination
T9539 24037-24038 , denotes ,
T9540 24039-24044 WDT denotes which
T9541 24045-24049 VBP denotes have
T9542 24050-24054 VBN denotes been
T9543 24055-24064 VBN denotes suggested
T9544 24065-24067 TO denotes to
T9545 24068-24074 VB denotes reduce
T9546 24075-24077 CC denotes or
T9547 24078-24085 VB denotes enhance
T9548 24086-24090 JJ denotes IKKγ
T9549 24091-24099 NN denotes activity
T9550 24099-24100 , denotes ,
T9551 24101-24113 RB denotes respectively
T9552 24114-24115 NNP denotes [
T9553 24115-24117 CD denotes 16
T9554 24117-24118 NNP denotes ]
T9555 24118-24119 , denotes ,
T9556 24120-24121 NNP denotes [
T9557 24121-24123 CD denotes 17
T9558 24123-24124 NNP denotes ]
T9559 24124-24125 . denotes .
T9560 24126-24129 DT denotes The
T9561 24130-24140 NNS denotes mechanisms
T9562 24141-24143 IN denotes by
T9563 24144-24149 WDT denotes which
T9564 24150-24154 NNP denotes HOIP
T9565 24155-24163 VBZ denotes mediates
T9566 24164-24175 NN denotes recruitment
T9567 24176-24178 IN denotes of
T9568 24179-24182 DT denotes the
T9569 24183-24186 NNP denotes IKK
T9570 24187-24194 NN denotes complex
T9571 24195-24197 TO denotes to
T9572 24198-24202 NNP denotes CD40
T9573 24203-24206 CC denotes and
T9574 24207-24209 IN denotes by
T9575 24210-24215 WDT denotes which
T9576 24216-24220 NNP denotes HOIP
T9577 24221-24223 VBZ denotes is
T9578 24224-24233 VBN denotes recruited
T9579 24234-24236 TO denotes to
T9580 24237-24241 NNP denotes CD40
T9581 24242-24248 VBP denotes remain
T9582 24249-24256 JJ denotes unclear
T9583 24256-24257 . denotes .
T9584 24258-24259 DT denotes A
T9585 24260-24268 JJ denotes previous
T9586 24269-24274 NN denotes study
T9587 24275-24284 VBZ denotes indicates
T9588 24285-24289 IN denotes that
T9589 24290-24294 NNP denotes HOIP
T9590 24295-24298 MD denotes may
T9591 24299-24306 VB denotes mediate
T9592 24307-24313 JJ denotes direct
T9593 24314-24322 NNS denotes contacts
T9594 24323-24327 IN denotes with
T9595 24328-24331 DT denotes the
T9596 24332-24335 NNP denotes IKK
T9597 24336-24343 JJ denotes complex
T9598 24344-24345 NNP denotes [
T9599 24345-24347 CD denotes 16
T9600 24347-24348 NNP denotes ]
T9601 24348-24349 , denotes ,
T9602 24350-24360 VBG denotes suggesting
T9603 24361-24365 IN denotes that
T9604 24366-24368 PRP denotes it
T9605 24369-24378 NNS denotes functions
T9606 24379-24381 IN denotes as
T9607 24382-24384 DT denotes an
T9608 24385-24392 NN denotes adaptor
T9609 24393-24396 IN denotes for
T9610 24397-24400 DT denotes the
T9611 24401-24412 NN denotes recruitment
T9612 24413-24415 IN denotes of
T9613 24416-24419 DT denotes the
T9614 24420-24423 NNP denotes IKK
T9615 24424-24431 NN denotes complex
T9616 24432-24434 TO denotes to
T9617 24435-24439 NNP denotes CD40
T9618 24439-24440 . denotes .
T9619 24441-24448 RB denotes However
T9620 24448-24449 , denotes ,
T9621 24450-24453 DT denotes the
T9622 24454-24463 NN denotes ubiquitin
T9623 24464-24470 NN denotes ligase
T9624 24471-24479 NN denotes activity
T9625 24480-24482 IN denotes of
T9626 24483-24487 NNP denotes HOIP
T9627 24488-24496 VBZ denotes suggests
T9628 24497-24501 IN denotes that
T9629 24502-24504 PRP denotes it
T9630 24505-24507 VBZ denotes is
T9631 24508-24512 JJR denotes more
T9632 24513-24517 IN denotes than
T9633 24518-24519 DT denotes a
T9634 24520-24526 JJ denotes simple
T9635 24527-24534 NN denotes adapter
T9636 24535-24543 NN denotes molecule
T9637 24543-24544 . denotes .
T9638 24545-24547 IN denotes As
T9639 24548-24552 NNP denotes IKKγ
T9640 24553-24560 VBZ denotes appears
T9641 24561-24568 JJ denotes capable
T9642 24569-24571 IN denotes of
T9643 24572-24579 JJ denotes binding
T9644 24580-24586 JJ denotes linear
T9645 24587-24600 NN denotes polyubiquitin
T9646 24601-24602 NNP denotes [
T9647 24602-24604 CD denotes 18
T9648 24604-24605 NNP denotes ]
T9649 24605-24606 , denotes ,
T9650 24607-24608 NNP denotes [
T9651 24608-24610 CD denotes 19
T9652 24610-24611 NNP denotes ]
T9653 24611-24612 , denotes ,
T9654 24613-24615 PRP denotes it
T9655 24616-24618 VBZ denotes is
T9656 24619-24627 JJ denotes possible
T9657 24628-24632 IN denotes that
T9658 24633-24637 NNP denotes HOIP
T9659 24638-24645 VBZ denotes directs
T9660 24646-24655 NN denotes formation
T9661 24656-24658 IN denotes of
T9662 24659-24665 JJ denotes linear
T9663 24666-24679 NN denotes polyubiquitin
T9664 24680-24686 NNS denotes chains
T9665 24687-24689 IN denotes on
T9666 24690-24691 DT denotes a
T9667 24692-24707 JJ denotes CD40-associated
T9668 24708-24714 NN denotes factor
T9669 24714-24715 , denotes ,
T9670 24716-24719 CC denotes and
T9671 24720-24722 PRP denotes it
T9672 24723-24725 VBZ denotes is
T9673 24726-24731 DT denotes these
T9674 24732-24738 NNS denotes chains
T9675 24739-24743 WDT denotes that
T9676 24744-24749 VBP denotes serve
T9677 24750-24752 TO denotes to
T9678 24753-24760 VB denotes recruit
T9679 24761-24765 NNP denotes IKKγ
T9680 24766-24768 TO denotes to
T9681 24769-24772 DT denotes the
T9682 24773-24777 NNP denotes CD40
T9683 24778-24787 VBG denotes signaling
T9684 24788-24795 NN denotes complex
T9685 24795-24796 . denotes .
T9686 24797-24800 DT denotes The
T9687 24801-24810 JJ denotes molecular
T9688 24811-24823 NNS denotes interactions
T9689 24824-24833 JJ denotes necessary
T9690 24834-24837 IN denotes for
T9691 24838-24849 NN denotes recruitment
T9692 24850-24852 IN denotes of
T9693 24853-24857 NNP denotes HOIP
T9694 24858-24864 PRP denotes itself
T9695 24865-24867 TO denotes to
T9696 24868-24871 DT denotes the
T9697 24872-24876 NNP denotes CD40
T9698 24877-24886 VBG denotes signaling
T9699 24887-24894 NN denotes complex
T9700 24895-24899 RB denotes also
T9701 24900-24906 VBP denotes remain
T9702 24907-24909 TO denotes to
T9703 24910-24912 VB denotes be
T9704 24913-24918 RB denotes fully
T9705 24919-24932 VBN denotes characterized
T9706 24932-24933 . denotes .
T9707 24934-24936 PRP denotes We
T9708 24937-24947 RB denotes previously
T9709 24948-24954 VBD denotes showed
T9710 24955-24959 IN denotes that
T9711 24960-24963 DT denotes the
T9712 24964-24975 NN denotes recruitment
T9713 24976-24978 IN denotes of
T9714 24979-24983 NNP denotes HOIP
T9715 24984-24986 TO denotes to
T9716 24987-24990 DT denotes the
T9717 24991-25000 VBG denotes signaling
T9718 25001-25008 NN denotes complex
T9719 25009-25011 VBZ denotes is
T9720 25012-25027 JJ denotes TRAF2-dependent
T9721 25028-25029 NN denotes [
T9722 25029-25030 CD denotes 8
T9723 25030-25031 NNP denotes ]
T9724 25031-25032 . denotes .
T9725 25033-25044 RB denotes Potentially
T9726 25044-25045 , denotes ,
T9727 25046-25051 CD denotes TRAF2
T9728 25052-25055 CC denotes and
T9729 25056-25060 NNP denotes HOIP
T9730 25061-25069 RB denotes directly
T9731 25070-25078 VBP denotes interact
T9732 25078-25079 , denotes ,
T9733 25080-25083 CC denotes but
T9734 25084-25086 PRP denotes it
T9735 25087-25089 VBZ denotes is
T9736 25090-25098 JJ denotes possible
T9737 25099-25103 IN denotes that
T9738 25104-25107 DT denotes the
T9739 25108-25117 NN denotes ubiquitin
T9740 25118-25124 NN denotes ligase
T9741 25125-25133 NN denotes activity
T9742 25134-25136 IN denotes of
T9743 25137-25142 CD denotes TRAF2
T9744 25143-25144 NNP denotes [
T9745 25144-25146 CD denotes 20
T9746 25146-25147 NNP denotes ]
T9747 25148-25149 -LRB- denotes (
T9748 25149-25151 CC denotes or
T9749 25152-25168 JJ denotes TRAF2-associated
T9750 25169-25177 NNS denotes proteins
T9751 25177-25178 , denotes ,
T9752 25179-25183 JJ denotes such
T9753 25184-25186 IN denotes as
T9754 25187-25190 DT denotes the
T9755 25191-25196 NNP denotes cIAPs
T9756 25197-25198 NNP denotes [
T9757 25198-25200 CD denotes 21
T9758 25200-25201 NNP denotes ]
T9759 25201-25202 -RRB- denotes )
T9760 25203-25212 VBZ denotes generates
T9761 25213-25223 JJ denotes K63-linked
T9762 25224-25237 NN denotes polyubiquitin
T9763 25238-25244 NNS denotes chains
T9764 25245-25247 TO denotes to
T9765 25248-25253 WDT denotes which
T9766 25254-25258 NNP denotes HOIP
T9767 25259-25262 MD denotes can
T9768 25263-25267 NN denotes bind
T9769 25268-25271 CC denotes and
T9770 25272-25276 RB denotes thus
T9771 25277-25286 JJ denotes associate
T9772 25287-25291 IN denotes with
T9773 25292-25295 DT denotes the
T9774 25296-25300 NNP denotes CD40
T9775 25301-25310 VBG denotes signaling
T9776 25311-25318 JJ denotes complex
T9777 25319-25320 NNP denotes [
T9778 25320-25322 CD denotes 22
T9779 25322-25323 NNP denotes ]
T9780 25323-25324 . denotes .
T9781 25325-25330 IN denotes While
T9782 25331-25334 PRP$ denotes our
T9783 25335-25343 JJ denotes previous
T9784 25344-25348 NN denotes work
T9785 25349-25358 VBD denotes indicated
T9786 25359-25360 DT denotes a
T9787 25361-25372 RB denotes potentially
T9788 25373-25382 JJ denotes important
T9789 25383-25387 NN denotes link
T9790 25388-25395 IN denotes between
T9791 25396-25401 CD denotes TRAF2
T9792 25402-25405 CC denotes and
T9793 25406-25410 NNP denotes HOIP
T9794 25411-25413 IN denotes in
T9795 25414-25418 NNP denotes CD40
T9796 25419-25428 VBG denotes signaling
T9797 25428-25429 , denotes ,
T9798 25430-25433 DT denotes the
T9799 25434-25441 NNS denotes signals
T9800 25442-25445 CC denotes and
T9801 25446-25455 NNS denotes functions
T9802 25456-25462 VBN denotes tested
T9803 25463-25467 RB denotes here
T9804 25468-25471 VBP denotes are
T9805 25472-25481 JJ denotes dependent
T9806 25482-25486 IN denotes upon
T9807 25487-25492 CD denotes TRAF6
T9808 25493-25495 RB denotes as
T9809 25496-25500 RB denotes well
T9810 25501-25503 IN denotes as
T9811 25504-25509 CD denotes TRAF2
T9812 25509-25510 . denotes .
T9813 25511-25513 IN denotes In
T9814 25514-25522 JJ denotes previous
T9815 25523-25534 NNS denotes experiments
T9816 25535-25539 IN denotes with
T9817 25540-25554 JJ denotes TRAF-deficient
T9818 25555-25558 NNP denotes A20
T9819 25558-25560 CD denotes .2
T9820 25560-25561 NNP denotes J
T9821 25562-25567 NNS denotes cells
T9822 25567-25568 , denotes ,
T9823 25569-25571 PRP denotes we
T9824 25572-25577 VBD denotes found
T9825 25578-25582 IN denotes that
T9826 25583-25586 DT denotes the
T9827 25587-25597 NN denotes activation
T9828 25598-25600 IN denotes of
T9829 25601-25606 NN denotes NF-κB
T9830 25607-25609 IN denotes by
T9831 25610-25614 NNP denotes CD40
T9832 25615-25620 MD denotes could
T9833 25621-25623 VB denotes be
T9834 25624-25632 VBN denotes mediated
T9835 25633-25635 IN denotes by
T9836 25636-25642 DT denotes either
T9837 25643-25648 CD denotes TRAF2
T9838 25649-25651 CC denotes or
T9839 25652-25657 CD denotes TRAF6
T9840 25657-25658 , denotes ,
T9841 25659-25664 IN denotes while
T9842 25665-25675 NN denotes activation
T9843 25676-25678 IN denotes of
T9844 25679-25682 NNP denotes JNK
T9845 25683-25685 IN denotes by
T9846 25686-25690 NNP denotes CD40
T9847 25691-25694 VBD denotes was
T9848 25695-25702 RB denotes largely
T9849 25703-25712 JJ denotes dependent
T9850 25713-25715 IN denotes on
T9851 25716-25721 CD denotes TRAF6
T9852 25722-25727 RB denotes alone
T9853 25728-25729 JJ denotes [
T9854 25729-25730 CD denotes 8
T9855 25730-25731 NN denotes ]
T9856 25731-25732 . denotes .
T9857 25733-25737 NNP denotes HOIP
T9858 25738-25748 NN denotes deficiency
T9859 25749-25760 VBZ denotes compromises
T9860 25761-25764 DT denotes the
T9861 25765-25778 JJ denotes CD40-mediated
T9862 25779-25789 NN denotes activation
T9863 25790-25792 IN denotes of
T9864 25793-25797 DT denotes both
T9865 25798-25803 NNP denotes NF-κB
T9866 25804-25807 CC denotes and
T9867 25808-25811 NNP denotes JNK
T9868 25811-25812 , denotes ,
T9869 25813-25823 VBG denotes indicating
T9870 25824-25828 IN denotes that
T9871 25829-25836 NNS denotes signals
T9872 25837-25845 VBN denotes mediated
T9873 25846-25848 IN denotes by
T9874 25849-25853 DT denotes both
T9875 25854-25859 CD denotes TRAF2
T9876 25860-25863 CC denotes and
T9877 25864-25869 CD denotes TRAF6
T9878 25870-25876 JJ denotes likely
T9879 25877-25881 NN denotes pass
T9880 25882-25889 IN denotes through
T9881 25890-25894 NNP denotes HOIP
T9882 25894-25895 . denotes .
T9883 25896-25899 PRP$ denotes Our
T9884 25900-25908 JJ denotes previous
T9885 25909-25913 NN denotes work
T9886 25914-25918 RB denotes also
T9887 25919-25931 VBD denotes demonstrated
T9888 25932-25936 IN denotes that
T9889 25937-25940 DT denotes the
T9890 25941-25954 JJ denotes CD40-mediated
T9891 25955-25965 NN denotes activation
T9892 25966-25968 IN denotes of
T9893 25969-25974 NNP denotes NF-κB
T9894 25975-25978 CC denotes and
T9895 25979-25982 NNP denotes JNK
T9896 25982-25983 , denotes ,
T9897 25984-25989 IN denotes while
T9898 25990-26005 JJ denotes TRAF6-dependent
T9899 26005-26006 , denotes ,
T9900 26007-26010 VBD denotes was
T9901 26011-26014 RB denotes not
T9902 26015-26026 VBN denotes compromised
T9903 26027-26029 IN denotes by
T9904 26030-26033 DT denotes the
T9905 26034-26044 NN denotes disruption
T9906 26045-26047 IN denotes of
T9907 26048-26051 DT denotes the
T9908 26052-26059 JJ denotes binding
T9909 26060-26064 NN denotes site
T9910 26065-26068 IN denotes for
T9911 26069-26074 CD denotes TRAF6
T9912 26075-26077 IN denotes in
T9913 26078-26081 DT denotes the
T9914 26082-26093 JJ denotes cytoplasmic
T9915 26094-26100 NN denotes domain
T9916 26101-26103 IN denotes of
T9917 26104-26108 CD denotes CD40
T9918 26109-26111 CC denotes or
T9919 26112-26120 NN denotes deletion
T9920 26121-26123 IN denotes of
T9921 26124-26127 DT denotes the
T9922 26128-26136 NN denotes receptor
T9923 26137-26144 JJ denotes binding
T9924 26145-26151 NN denotes domain
T9925 26152-26153 -LRB- denotes (
T9926 26153-26156 DT denotes the
T9927 26157-26163 NNP denotes TRAF-C
T9928 26164-26170 NN denotes domain
T9929 26170-26171 -RRB- denotes )
T9930 26172-26174 IN denotes in
T9931 26175-26180 CD denotes TRAF6
T9932 26181-26182 NN denotes [
T9933 26182-26183 CD denotes 8
T9934 26183-26184 NNP denotes ]
T9935 26184-26185 . denotes .
T9936 26186-26191 DT denotes These
T9937 26192-26204 NNS denotes observations
T9938 26205-26213 VBP denotes indicate
T9939 26214-26218 IN denotes that
T9940 26219-26224 CD denotes TRAF6
T9941 26225-26229 MD denotes need
T9942 26230-26233 RB denotes not
T9943 26234-26242 RB denotes directly
T9944 26243-26247 NN denotes bind
T9945 26248-26252 CD denotes CD40
T9946 26253-26255 IN denotes in
T9947 26256-26261 NN denotes order
T9948 26262-26264 TO denotes to
T9949 26265-26272 VB denotes mediate
T9950 26273-26280 JJ denotes certain
T9951 26281-26288 NNS denotes signals
T9952 26288-26289 , denotes ,
T9953 26290-26300 VBG denotes suggesting
T9954 26301-26304 DT denotes the
T9955 26305-26313 NN denotes assembly
T9956 26314-26316 IN denotes of
T9957 26317-26318 DT denotes a
T9958 26319-26328 VBG denotes signaling
T9959 26329-26336 NN denotes complex
T9960 26337-26340 RB denotes not
T9961 26341-26349 RB denotes directly
T9962 26350-26360 VBN denotes associated
T9963 26361-26365 IN denotes with
T9964 26366-26369 DT denotes the
T9965 26370-26378 NN denotes receptor
T9966 26378-26379 . denotes .
T9967 26380-26382 IN denotes If
T9968 26383-26387 PDT denotes such
T9969 26388-26389 DT denotes a
T9970 26390-26397 JJ denotes complex
T9971 26398-26404 VBZ denotes exists
T9972 26404-26405 , denotes ,
T9973 26406-26409 PRP$ denotes our
T9974 26410-26417 NNS denotes results
T9975 26418-26426 VBP denotes indicate
T9976 26427-26431 IN denotes that
T9977 26432-26435 DT denotes the
T9978 26436-26443 NN denotes absence
T9979 26444-26446 IN denotes of
T9980 26447-26451 NNP denotes HOIP
T9981 26452-26463 VBZ denotes compromises
T9982 26464-26467 PRP$ denotes its
T9983 26468-26476 NN denotes function
T9984 26477-26479 RB denotes as
T9985 26480-26484 RB denotes well
T9986 26484-26485 . denotes .
T9987 26486-26494 IN denotes Although
T9988 26495-26498 DT denotes the
T9989 26499-26510 NNS denotes experiments
T9990 26511-26520 VBN denotes presented
T9991 26521-26525 RB denotes here
T9992 26526-26531 VB denotes focus
T9993 26532-26534 IN denotes on
T9994 26535-26539 NNP denotes CD40
T9995 26539-26540 , denotes ,
T9996 26541-26544 PRP$ denotes our
T9997 26545-26552 NNS denotes results
T9998 26553-26556 CC denotes and
T9999 26557-26562 DT denotes those
T10000 26563-26565 IN denotes of
T10001 26566-26571 JJ denotes other
T10002 26572-26578 NNS denotes groups
T10003 26579-26580 NNP denotes [
T10004 26580-26582 CD denotes 16
T10005 26582-26583 NNP denotes ]
T10006 26583-26584 , denotes ,
T10007 26585-26586 NNP denotes [
T10008 26586-26588 CD denotes 22
T10009 26588-26589 JJ denotes ]
T10010 26590-26597 NN denotes support
T10011 26598-26601 DT denotes the
T10012 26602-26613 NN denotes possibility
T10013 26614-26618 WDT denotes that
T10014 26619-26623 NNP denotes HOIP
T10015 26624-26626 VBZ denotes is
T10016 26627-26636 JJ denotes important
T10017 26637-26639 IN denotes in
T10018 26640-26644 JJ denotes many
T10019 26645-26654 VBG denotes signaling
T10020 26655-26663 NNS denotes pathways
T10021 26664-26666 IN denotes in
T10022 26667-26672 WDT denotes which
T10023 26673-26678 CD denotes TRAF2
T10024 26679-26681 CC denotes or
T10025 26682-26687 CD denotes TRAF6
T10026 26688-26691 VBP denotes are
T10027 26692-26700 VBN denotes involved
T10028 26700-26701 , denotes ,
T10029 26702-26711 VBG denotes including
T10030 26712-26717 DT denotes those
T10031 26718-26728 VBN denotes associated
T10032 26729-26733 IN denotes with
T10033 26734-26741 JJ denotes various
T10034 26742-26749 NNS denotes members
T10035 26750-26752 IN denotes of
T10036 26753-26756 DT denotes the
T10037 26757-26760 NNP denotes TNF
T10038 26761-26769 NN denotes receptor
T10039 26770-26781 RB denotes superfamily
T10040 26782-26785 CC denotes and
T10041 26786-26789 DT denotes the
T10042 26790-26799 JJ denotes Toll-like
T10043 26800-26809 NNS denotes receptors
T10044 26809-26810 . denotes .
T10045 26811-26814 DT denotes The
T10046 26815-26824 JJ denotes potential
T10047 26825-26835 NN denotes importance
T10048 26836-26838 IN denotes of
T10049 26839-26843 NNP denotes HOIP
T10050 26844-26846 IN denotes in
T10051 26847-26853 JJ denotes immune
T10052 26854-26862 NN denotes function
T10053 26863-26866 CC denotes and
T10054 26867-26871 NNP denotes TNFR
T10055 26872-26878 NN denotes family
T10056 26879-26888 VBG denotes signaling
T10057 26889-26891 VBZ denotes is
T10058 26892-26899 JJ denotes further
T10059 26900-26909 VBN denotes supported
T10060 26910-26912 IN denotes by
T10061 26913-26916 DT denotes the
T10062 26917-26923 JJ denotes recent
T10063 26924-26933 NN denotes discovery
T10064 26934-26938 WDT denotes that
T10065 26939-26943 NNP denotes HOIP
T10066 26944-26953 VBZ denotes interacts
T10067 26954-26958 IN denotes with
T10068 26959-26960 DT denotes a
T10069 26961-26968 NN denotes protein
T10070 26969-26974 VBN denotes known
T10071 26975-26977 IN denotes as
T10072 26978-26985 NNP denotes SHARPIN
T10073 26986-26987 -LRB- denotes (
T10074 26987-27003 JJ denotes SHANK-associated
T10075 27004-27006 NNP denotes RH
T10076 27007-27013 NN denotes domain
T10077 27014-27025 VBG denotes interacting
T10078 27026-27033 NN denotes protein
T10079 27034-27036 IN denotes in
T10080 27037-27049 JJ denotes postsynaptic
T10081 27050-27057 NN denotes density
T10082 27057-27058 -RRB- denotes )
T10083 27058-27059 , denotes ,
T10084 27060-27065 WDT denotes which
T10085 27066-27073 VBZ denotes appears
T10086 27074-27081 JJ denotes capable
T10087 27082-27084 IN denotes of
T10088 27085-27092 VBG denotes working
T10089 27093-27101 RB denotes together
T10090 27102-27106 IN denotes with
T10091 27107-27111 NNP denotes HOIP
T10092 27112-27115 CC denotes and
T10093 27116-27120 NNP denotes HOIL
T10094 27121-27123 TO denotes to
T10095 27124-27131 VB denotes mediate
T10096 27132-27135 DT denotes the
T10097 27136-27144 NN denotes assembly
T10098 27145-27147 IN denotes of
T10099 27148-27154 JJ denotes linear
T10100 27155-27168 NN denotes polyubiquitin
T10101 27169-27170 NNP denotes [
T10102 27170-27172 CD denotes 14
T10103 27172-27173 NNP denotes ]
T10104 27173-27174 , denotes ,
T10105 27175-27176 NNP denotes [
T10106 27176-27178 CD denotes 23
T10107 27178-27179 NNP denotes ]
T10108 27179-27180 , denotes ,
T10109 27181-27182 NNP denotes [
T10110 27182-27184 CD denotes 24
T10111 27184-27185 NNP denotes ]
T10112 27185-27186 . denotes .
T10113 27187-27200 RB denotes Interestingly
T10114 27200-27201 , denotes ,
T10115 27202-27206 NNS denotes mice
T10116 27207-27211 IN denotes with
T10117 27212-27213 DT denotes a
T10118 27214-27225 JJ denotes spontaneous
T10119 27226-27234 NN denotes mutation
T10120 27235-27237 IN denotes in
T10121 27238-27241 DT denotes the
T10122 27242-27246 NN denotes gene
T10123 27247-27255 VBG denotes encoding
T10124 27256-27263 NNP denotes SHARPIN
T10125 27264-27265 -LRB- denotes (
T10126 27265-27272 JJ denotes chronic
T10127 27273-27286 JJ denotes proliferative
T10128 27287-27297 NNS denotes dermatitis
T10129 27298-27299 -LRB- denotes (
T10130 27299-27303 NN denotes cpdm
T10131 27303-27304 -RRB- denotes )
T10132 27305-27309 NNS denotes mice
T10133 27309-27310 -RRB- denotes )
T10134 27311-27318 VBP denotes exhibit
T10135 27319-27326 JJ denotes chronic
T10136 27327-27339 NN denotes inflammation
T10137 27340-27342 IN denotes of
T10138 27343-27346 DT denotes the
T10139 27347-27351 NN denotes skin
T10140 27352-27355 CC denotes and
T10141 27356-27364 JJ denotes internal
T10142 27365-27371 NNS denotes organs
T10143 27371-27372 , denotes ,
T10144 27373-27382 JJ denotes defective
T10145 27383-27394 NN denotes development
T10146 27395-27397 IN denotes of
T10147 27398-27407 JJ denotes secondary
T10148 27408-27416 JJ denotes lymphoid
T10149 27417-27423 NN denotes tissue
T10150 27423-27424 , denotes ,
T10151 27425-27428 CC denotes and
T10152 27429-27436 NNS denotes defects
T10153 27437-27439 IN denotes in
T10154 27440-27443 DT denotes the
T10155 27444-27454 NN denotes production
T10156 27455-27457 IN denotes of
T10157 27458-27466 VBN denotes switched
T10158 27467-27481 NN denotes immunoglobulin
T10159 27482-27490 NNS denotes isotypes
T10160 27491-27492 NNP denotes [
T10161 27492-27494 CD denotes 17
T10162 27494-27495 NNP denotes ]
T10163 27495-27496 , denotes ,
T10164 27497-27498 NNP denotes [
T10165 27498-27500 CD denotes 25
T10166 27500-27501 NNP denotes ]
T10167 27501-27502 . denotes .
T10168 27503-27506 DT denotes The
T10169 27507-27517 RB denotes apparently
T10170 27518-27526 JJ denotes intimate
T10171 27527-27537 JJ denotes functional
T10172 27538-27542 NN denotes link
T10173 27543-27550 IN denotes between
T10174 27551-27558 NNP denotes SHARPIN
T10175 27559-27562 CC denotes and
T10176 27563-27567 NNP denotes HOIP
T10177 27568-27576 RB denotes strongly
T10178 27577-27585 VBZ denotes suggests
T10179 27586-27590 IN denotes that
T10180 27591-27593 IN denotes at
T10181 27594-27599 JJS denotes least
T10182 27600-27604 NN denotes part
T10183 27605-27607 IN denotes of
T10184 27608-27611 DT denotes the
T10185 27612-27616 NN denotes cpdm
T10186 27617-27626 NN denotes phenotype
T10187 27627-27632 VBZ denotes stems
T10188 27633-27637 IN denotes from
T10189 27638-27645 NNS denotes defects
T10190 27646-27648 IN denotes in
T10191 27649-27652 DT denotes the
T10192 27653-27663 NN denotes regulation
T10193 27664-27666 CC denotes or
T10194 27667-27675 NN denotes function
T10195 27676-27678 IN denotes of
T10196 27679-27683 NNP denotes HOIP
T10197 27683-27684 . denotes .
T11924 27709-27713 NN denotes Cell
T11925 27714-27719 NNS denotes lines
T11926 27720-27723 DT denotes The
T11927 27724-27729 NN denotes mouse
T11928 27730-27731 NNP denotes B
T11929 27732-27736 NN denotes cell
T11930 27737-27741 NN denotes line
T11931 27742-27745 NNP denotes A20
T11932 27745-27747 CD denotes .2
T11933 27747-27748 NNP denotes J
T11934 27749-27752 VBZ denotes has
T11935 27753-27757 VBN denotes been
T11936 27758-27768 RB denotes previously
T11937 27769-27778 VBN denotes described
T11938 27779-27780 NNP denotes [
T11939 27780-27782 CD denotes 26
T11940 27782-27783 NNP denotes ]
T11941 27783-27784 , denotes ,
T11942 27785-27786 NNP denotes [
T11943 27786-27788 CD denotes 27
T11944 27788-27789 NNP denotes ]
T11945 27789-27790 . denotes .
T11946 27791-27796 NNS denotes Cells
T11947 27797-27801 VBD denotes were
T11948 27802-27807 VBN denotes grown
T11949 27808-27810 IN denotes in
T11950 27811-27815 NNP denotes RPMI
T11951 27816-27820 CD denotes 1640
T11952 27820-27821 , denotes ,
T11953 27822-27824 CD denotes 10
T11954 27824-27825 NN denotes %
T11955 27826-27829 NNP denotes FCS
T11956 27829-27830 , denotes ,
T11957 27831-27833 CD denotes 10
T11958 27834-27836 NNP denotes µM
T11959 27837-27841 NNP denotes 2-ME
T11960 27841-27842 , denotes ,
T11961 27843-27844 CD denotes 2
T11962 27845-27847 NNP denotes mM
T11963 27848-27859 NNP denotes L-glutamine
T11964 27859-27860 , denotes ,
T11965 27861-27864 CC denotes and
T11966 27865-27876 NNS denotes antibiotics
T11967 27876-27877 . denotes .
T11968 27878-27882 CD denotes HI-5
T11969 27883-27889 JJ denotes insect
T11970 27890-27895 NNS denotes cells
T11971 27896-27897 -LRB- denotes (
T11972 27897-27907 NNP denotes Invitrogen
T11973 27907-27908 -RRB- denotes )
T11974 27909-27919 VBG denotes expressing
T11975 27920-27925 CD denotes CD154
T11976 27926-27930 VBP denotes have
T11977 27931-27935 VBN denotes been
T11978 27936-27945 VBN denotes described
T11979 27946-27947 NNP denotes [
T11980 27947-27949 CD denotes 28
T11981 27949-27950 NNP denotes ]
T11982 27950-27951 . denotes .
T12059 27953-27963 NNPS denotes Antibodies
T12060 27964-27969 NNP denotes Mouse
T12061 27970-27980 JJ denotes anti-birch
T12062 27981-27989 NN denotes profilin
T12063 27990-27998 NN denotes antibody
T12064 27999-28000 -LRB- denotes (
T12065 28000-28003 NNP denotes 4A6
T12066 28004-28005 NNP denotes [
T12067 28005-28007 CD denotes 29
T12068 28007-28008 NNP denotes ]
T12069 28008-28009 -RRB- denotes )
T12070 28009-28010 , denotes ,
T12071 28011-28014 NN denotes rat
T12072 28015-28025 JJ denotes anti-mouse
T12073 28026-28030 NNP denotes CD40
T12074 28031-28032 -LRB- denotes (
T12075 28032-28036 NNP denotes 1C10
T12076 28037-28038 NNP denotes [
T12077 28038-28040 CD denotes 30
T12078 28040-28041 NNP denotes ]
T12079 28041-28042 -RRB- denotes )
T12080 28043-28046 CC denotes and
T12081 28047-28048 DT denotes a
T12082 28049-28052 NN denotes rat
T12083 28053-28060 NN denotes isotype
T12084 28061-28068 NN denotes control
T12085 28069-28077 NN denotes antibody
T12086 28078-28079 -LRB- denotes (
T12087 28079-28084 NNP denotes mAb72
T12088 28084-28085 , denotes ,
T12089 28086-28099 NNP denotes Developmental
T12090 28100-28107 NNP denotes Studies
T12091 28108-28117 NNP denotes Hybridoma
T12092 28118-28122 NNP denotes Bank
T12093 28122-28123 , denotes ,
T12094 28124-28134 NNP denotes University
T12095 28135-28137 IN denotes of
T12096 28138-28142 NNP denotes Iowa
T12097 28142-28143 , denotes ,
T12098 28144-28148 NNP denotes Iowa
T12099 28149-28153 NNP denotes City
T12100 28153-28154 , denotes ,
T12101 28155-28157 NNP denotes IA
T12102 28157-28158 -RRB- denotes )
T12103 28159-28163 VBD denotes were
T12104 28164-28172 VBN denotes isolated
T12105 28173-28177 IN denotes from
T12106 28178-28187 NN denotes hybridoma
T12107 28188-28200 NNS denotes supernatants
T12108 28200-28201 . denotes .
T12109 28202-28214 JJ denotes FITC-labeled
T12110 28215-28225 JJ denotes anti-mouse
T12111 28226-28230 NN denotes CD80
T12112 28231-28234 CC denotes and
T12113 28235-28237 DT denotes an
T12114 28238-28245 NN denotes isotype
T12115 28246-28253 NN denotes control
T12116 28254-28256 NNP denotes Ab
T12117 28257-28261 VBD denotes were
T12118 28262-28266 IN denotes from
T12119 28267-28279 NNS denotes eBiosciences
T12120 28279-28280 . denotes .
T12121 28281-28287 NN denotes Rabbit
T12122 28288-28297 JJ denotes anti-HOIP
T12123 28298-28300 NNP denotes Ab
T12124 28301-28302 NNP denotes [
T12125 28302-28303 CD denotes 6
T12126 28303-28304 NNP denotes ]
T12127 28305-28308 VBD denotes was
T12128 28309-28312 DT denotes the
T12129 28313-28317 NN denotes kind
T12130 28318-28322 NN denotes gift
T12131 28323-28325 IN denotes of
T12132 28326-28329 NNP denotes Dr.
T12133 28330-28335 NNP denotes Betty
T12134 28336-28342 NNP denotes Eipper
T12135 28343-28344 -LRB- denotes (
T12136 28344-28354 NNP denotes University
T12137 28355-28357 IN denotes of
T12138 28358-28369 NNP denotes Connecticut
T12139 28370-28376 NNP denotes Health
T12140 28377-28383 NNP denotes Center
T12141 28383-28384 , denotes ,
T12142 28385-28395 NNP denotes Farmington
T12143 28395-28396 , denotes ,
T12144 28397-28408 NNP denotes Connecticut
T12145 28408-28409 -RRB- denotes )
T12146 28409-28410 . denotes .
T12147 28411-28415 NNP denotes Goat
T12148 28416-28424 JJ denotes anti-rat
T12149 28425-28428 NNP denotes IgG
T12150 28428-28429 , denotes ,
T12151 28430-28433 CC denotes and
T12152 28434-28445 JJ denotes HRP-labeled
T12153 28446-28455 JJ denotes secondary
T12154 28456-28459 NNS denotes Abs
T12155 28460-28464 VBD denotes were
T12156 28465-28469 IN denotes from
T12157 28470-28477 NNP denotes Jackson
T12158 28478-28492 NNP denotes ImmunoResearch
T12159 28493-28505 NNPS denotes Laboratories
T12160 28505-28506 , denotes ,
T12161 28507-28511 NNP denotes Inc.
T12162 28567-28568 . denotes .
T12332 28570-28577 JJ denotes Somatic
T12333 28578-28582 NN denotes cell
T12334 28583-28587 NN denotes gene
T12335 28588-28597 VBG denotes targeting
T12336 28598-28601 DT denotes The
T12337 28602-28612 NN denotes generation
T12338 28613-28615 IN denotes of
T12339 28616-28630 JJ denotes HOIP-deficient
T12340 28631-28636 NNS denotes cells
T12341 28637-28640 VBD denotes was
T12342 28641-28653 VBN denotes accomplished
T12343 28654-28659 VBG denotes using
T12344 28660-28661 DT denotes a
T12345 28662-28672 RB denotes homologous
T12346 28673-28686 NN denotes recombination
T12347 28687-28695 NN denotes approach
T12348 28696-28705 VBD denotes described
T12349 28706-28716 RB denotes previously
T12350 28717-28718 JJ denotes [
T12351 28718-28719 CD denotes 7
T12352 28719-28720 NNP denotes ]
T12353 28720-28721 , denotes ,
T12354 28722-28723 NNP denotes [
T12355 28723-28724 CD denotes 8
T12356 28724-28725 NNP denotes ]
T12357 28725-28726 . denotes .
T12358 28727-28735 NNS denotes Segments
T12359 28736-28738 IN denotes of
T12360 28739-28744 JJ denotes Rnf31
T12361 28745-28749 NN denotes gene
T12362 28750-28758 NN denotes sequence
T12363 28759-28763 VBN denotes used
T12364 28764-28766 IN denotes in
T12365 28767-28770 DT denotes the
T12366 28771-28780 VBG denotes targeting
T12367 28781-28790 VB denotes construct
T12368 28791-28792 -LRB- denotes (
T12369 28792-28796 NNP denotes Fig.
T12370 28797-28799 NNP denotes 1A
T12371 28799-28800 -RRB- denotes )
T12372 28801-28805 VBD denotes were
T12373 28806-28815 VBN denotes amplified
T12374 28816-28818 IN denotes by
T12375 28819-28822 NNP denotes PCR
T12376 28823-28827 IN denotes from
T12377 28828-28831 NNP denotes A20
T12378 28831-28833 CD denotes .2
T12379 28833-28834 NNP denotes J
T12380 28835-28842 JJ denotes genomic
T12381 28843-28846 NN denotes DNA
T12382 28846-28847 . denotes .
T12383 28848-28851 DT denotes The
T12384 28852-28867 NN denotes oligonucleotide
T12385 28868-28875 NNS denotes primers
T12386 28876-28880 VBN denotes used
T12387 28881-28883 TO denotes to
T12388 28884-28892 VB denotes generate
T12389 28893-28896 DT denotes the
T12390 28897-28898 CD denotes 5
T12391 28898-28899 NN denotes ′
T12392 28900-28905 NN denotes flank
T12393 28906-28907 -LRB- denotes (
T12394 28907-28911 CD denotes 1224
T12395 28912-28914 NN denotes bp
T12396 28914-28915 -RRB- denotes )
T12397 28916-28920 VBD denotes were
T12398 28921-28922 CD denotes 5
T12399 28922-28923 SYM denotes ′
T12400 28923-28924 : denotes -
T12401 28924-28954 JJ denotes ttttctagagcggtggcttaagtgaccc-3
T12402 28954-28955 NN denotes ′
T12403 28956-28959 CC denotes and
T12404 28960-28961 CD denotes 5
T12405 28961-28962 SYM denotes ′
T12406 28962-28963 : denotes -
T12407 28963-28996 JJ denotes tattctagatgcagcatctgagaaagcaagc-3
T12408 28996-28997 NN denotes ′
T12409 28997-28998 . denotes .
T12410 28999-29002 DT denotes The
T12411 29003-29004 CD denotes 3
T12412 29004-29005 NN denotes ′
T12413 29006-29011 NN denotes flank
T12414 29012-29013 -LRB- denotes (
T12415 29013-29017 CD denotes 6032
T12416 29018-29020 NN denotes bp
T12417 29020-29021 -RRB- denotes )
T12418 29022-29029 NNS denotes primers
T12419 29030-29034 VBD denotes were
T12420 29035-29036 CD denotes 5
T12421 29036-29037 SYM denotes ′
T12422 29037-29038 : denotes -
T12423 29038-29079 JJ denotes aaaaccggtgtatgcttctttacgggagaaaaatattag-3
T12424 29079-29080 NN denotes ′
T12425 29081-29084 CC denotes and
T12426 29085-29086 CD denotes 5
T12427 29086-29087 SYM denotes ′
T12428 29087-29088 : denotes -
T12429 29088-29123 JJ denotes tataccggtatgaagccaaaggaacactgagag-3
T12430 29123-29124 NN denotes ′
T12431 29124-29125 . denotes .
T12432 29126-29137 NN denotes Restriction
T12433 29138-29150 NN denotes endonuclease
T12434 29151-29156 NNS denotes sites
T12435 29157-29159 IN denotes in
T12436 29160-29163 DT denotes the
T12437 29164-29179 NN denotes oligonucleotide
T12438 29180-29187 VBZ denotes primers
T12439 29188-29195 VBN denotes allowed
T12440 29196-29205 NN denotes insertion
T12441 29206-29208 IN denotes of
T12442 29209-29212 DT denotes the
T12443 29213-29216 NNP denotes PCR
T12444 29217-29225 NNS denotes products
T12445 29226-29230 IN denotes into
T12446 29231-29234 DT denotes the
T12447 29235-29244 VBG denotes targeting
T12448 29245-29251 NN denotes vector
T12449 29251-29252 . denotes .
T12450 29253-29256 CD denotes A20
T12451 29256-29258 CD denotes .2
T12452 29258-29259 NNP denotes J
T12453 29260-29265 NNS denotes cells
T12454 29266-29270 VBD denotes were
T12455 29271-29282 VBN denotes transfected
T12456 29283-29284 -LRB- denotes (
T12457 29284-29286 IN denotes by
T12458 29287-29302 NN denotes electroporation
T12459 29303-29304 NN denotes [
T12460 29304-29305 CD denotes 7
T12461 29305-29306 NNP denotes ]
T12462 29306-29307 -RRB- denotes )
T12463 29308-29312 IN denotes with
T12464 29313-29316 DT denotes the
T12465 29317-29326 VBG denotes targeting
T12466 29327-29336 VB denotes construct
T12467 29337-29340 CC denotes and
T12468 29341-29350 VBN denotes subcloned
T12469 29351-29353 IN denotes in
T12470 29354-29360 NN denotes medium
T12471 29361-29371 VBG denotes containing
T12472 29372-29375 CD denotes 600
T12473 29376-29378 JJ denotes µg
T12474 29378-29379 NN denotes /
T12475 29379-29381 NN denotes ml
T12476 29382-29386 NNP denotes G418
T12477 29387-29394 NN denotes sulfate
T12478 29394-29395 . denotes .
T12479 29396-29406 JJ denotes Homologous
T12480 29407-29420 NN denotes recombination
T12481 29421-29423 IN denotes in
T12482 29424-29438 JJ denotes G418-resistant
T12483 29439-29445 NNS denotes clones
T12484 29446-29449 VBD denotes was
T12485 29450-29458 VBN denotes detected
T12486 29459-29461 IN denotes by
T12487 29462-29465 NNP denotes PCR
T12488 29466-29468 IN denotes of
T12489 29469-29476 JJ denotes genomic
T12490 29477-29480 NN denotes DNA
T12491 29480-29481 , denotes ,
T12492 29482-29484 IN denotes as
T12493 29485-29494 VBN denotes described
T12494 29495-29496 NN denotes [
T12495 29496-29497 CD denotes 7
T12496 29497-29498 NNP denotes ]
T12497 29498-29499 . denotes .
T12498 29500-29515 NNP denotes Oligonucleotide
T12499 29516-29523 NNS denotes primers
T12500 29524-29528 VBD denotes used
T12501 29529-29532 IN denotes for
T12502 29533-29542 VBG denotes screening
T12503 29543-29547 VBD denotes were
T12504 29548-29549 CD denotes 5
T12505 29549-29550 SYM denotes ′
T12506 29550-29551 : denotes -
T12507 29551-29579 JJ denotes cttcctgatctcagctttaccgtcac-3
T12508 29579-29580 NN denotes ′
T12509 29581-29582 -LRB- denotes (
T12510 29582-29592 RB denotes homologous
T12511 29593-29595 TO denotes to
T12512 29596-29603 JJ denotes genomic
T12513 29604-29612 NN denotes sequence
T12514 29612-29613 : denotes ;
T12515 29614-29625 JJ denotes approximate
T12516 29626-29634 NN denotes position
T12517 29635-29640 VBD denotes noted
T12518 29641-29643 IN denotes in
T12519 29644-29648 NNP denotes Fig.
T12520 29649-29650 CD denotes 1
T12521 29650-29651 -RRB- denotes )
T12522 29652-29655 CC denotes and
T12523 29656-29657 CD denotes 5
T12524 29657-29658 SYM denotes ′
T12525 29658-29659 : denotes -
T12526 29659-29682 JJ denotes caatccatcttgttcagccat-3
T12527 29682-29683 NN denotes ′
T12528 29684-29685 -LRB- denotes (
T12529 29685-29695 RB denotes homologous
T12530 29696-29698 TO denotes to
T12531 29699-29707 NN denotes sequence
T12532 29708-29710 IN denotes in
T12533 29711-29715 NNP denotes NeoR
T12534 29715-29716 -RRB- denotes )
T12535 29716-29717 . denotes .
T12536 29718-29724 NNS denotes Clones
T12537 29725-29727 IN denotes in
T12538 29728-29733 WDT denotes which
T12539 29734-29737 CD denotes one
T12540 29738-29742 NN denotes copy
T12541 29743-29745 IN denotes of
T12542 29746-29751 NNP denotes Rnf31
T12543 29752-29755 VBD denotes had
T12544 29756-29760 VBN denotes been
T12545 29761-29770 VBN denotes disrupted
T12546 29771-29775 VBD denotes were
T12547 29776-29787 RB denotes transiently
T12548 29788-29799 VBN denotes transfected
T12549 29800-29804 IN denotes with
T12550 29805-29807 DT denotes an
T12551 29808-29818 NN denotes expression
T12552 29819-29826 NN denotes plasmid
T12553 29827-29835 VBG denotes encoding
T12554 29836-29839 NNP denotes Cre
T12555 29839-29840 , denotes ,
T12556 29841-29843 IN denotes in
T12557 29844-29849 NN denotes order
T12558 29850-29852 TO denotes to
T12559 29853-29859 VB denotes remove
T12560 29860-29864 NNP denotes NeoR
T12561 29864-29865 . denotes .
T12562 29866-29880 JJ denotes G418-sensitive
T12563 29881-29890 NNS denotes subclones
T12564 29891-29895 VBD denotes were
T12565 29896-29905 VBN denotes subjected
T12566 29906-29908 TO denotes to
T12567 29909-29910 DT denotes a
T12568 29911-29917 JJ denotes second
T12569 29918-29923 NN denotes round
T12570 29924-29926 IN denotes of
T12571 29927-29936 VBG denotes targeting
T12572 29937-29939 TO denotes to
T12573 29940-29947 VB denotes disrupt
T12574 29948-29951 DT denotes the
T12575 29952-29961 VBG denotes remaining
T12576 29962-29966 NN denotes copy
T12577 29967-29969 IN denotes of
T12578 29970-29975 NNP denotes Rnf31
T12579 29975-29976 . denotes .
T12580 29977-29991 JJ denotes G418-resistant
T12581 29992-29998 NNS denotes clones
T12582 29999-30003 VBD denotes were
T12583 30004-30010 VBN denotes tested
T12584 30011-30014 IN denotes for
T12585 30015-30025 RB denotes homologous
T12586 30026-30039 NN denotes recombination
T12587 30040-30042 IN denotes by
T12588 30043-30046 NNP denotes PCR
T12589 30047-30050 CC denotes and
T12590 30051-30055 NNP denotes HOIP
T12591 30056-30063 NN denotes protein
T12592 30064-30074 NN denotes expression
T12593 30075-30077 IN denotes by
T12594 30078-30085 JJ denotes Western
T12595 30086-30090 NN denotes blot
T12596 30090-30091 . denotes .
T12911 30093-30103 NNP denotes Retroviral
T12912 30104-30116 NN denotes transduction
T12913 30117-30118 DT denotes A
T12914 30119-30129 JJ denotes retroviral
T12915 30130-30136 NN denotes vector
T12916 30137-30138 -LRB- denotes (
T12917 30138-30142 NNP denotes pMIP
T12918 30142-30143 -RRB- denotes )
T12919 30144-30147 VBD denotes was
T12920 30148-30152 VBN denotes used
T12921 30153-30156 IN denotes for
T12922 30157-30160 DT denotes the
T12923 30161-30167 JJ denotes stable
T12924 30168-30180 NN denotes transduction
T12925 30181-30183 IN denotes of
T12926 30184-30189 NNS denotes cells
T12927 30190-30194 IN denotes with
T12928 30195-30199 NNP denotes HOIP
T12929 30200-30203 CC denotes and
T12930 30204-30208 NNP denotes IKKγ
T12931 30209-30213 NNP denotes cDNA
T12932 30214-30224 NNS denotes constructs
T12933 30224-30225 . denotes .
T12934 30226-30230 NN denotes pMIP
T12935 30231-30234 VBD denotes was
T12936 30235-30246 VBN denotes constructed
T12937 30247-30249 IN denotes by
T12938 30250-30259 VBG denotes replacing
T12939 30260-30263 DT denotes the
T12940 30264-30268 NNP denotes IRES
T12941 30269-30272 CC denotes and
T12942 30273-30276 NNP denotes GFP
T12943 30277-30285 VBG denotes encoding
T12944 30286-30295 NNS denotes sequences
T12945 30296-30298 IN denotes in
T12946 30299-30303 NNP denotes pMIG
T12947 30304-30305 NNP denotes [
T12948 30305-30307 CD denotes 31
T12949 30307-30308 NN denotes ]
T12950 30309-30313 IN denotes with
T12951 30314-30317 DT denotes the
T12952 30318-30322 NNP denotes IRES
T12953 30323-30326 CC denotes and
T12954 30327-30336 JJ denotes puromycin
T12955 30337-30347 NN denotes resistance
T12956 30348-30352 NN denotes gene
T12957 30353-30357 IN denotes from
T12958 30358-30368 NNP denotes pIRESpuro2
T12959 30369-30370 -LRB- denotes (
T12960 30370-30378 NNP denotes Clontech
T12961 30378-30379 -RRB- denotes )
T12962 30379-30380 . denotes .
T12963 30381-30392 JJ denotes FLAG-tagged
T12964 30393-30398 NN denotes mouse
T12965 30399-30403 NNP denotes HOIP
T12966 30404-30408 NNP denotes cDNA
T12967 30409-30412 VBD denotes was
T12968 30413-30421 VBN denotes inserted
T12969 30422-30426 IN denotes into
T12970 30427-30430 DT denotes the
T12971 30431-30439 JJ denotes multiple
T12972 30440-30447 NN denotes cloning
T12973 30448-30452 NN denotes site
T12974 30453-30455 IN denotes of
T12975 30456-30460 NNP denotes pMIP
T12976 30460-30461 . denotes .
T12977 30462-30463 DT denotes A
T12978 30464-30468 NN denotes pMIP
T12979 30469-30478 VBP denotes construct
T12980 30479-30487 VBG denotes encoding
T12981 30488-30493 NN denotes mouse
T12982 30494-30498 NNP denotes IKKγ
T12983 30499-30503 IN denotes with
T12984 30504-30506 DT denotes an
T12985 30507-30512 JJ denotes amino
T12986 30513-30521 NN denotes terminal
T12987 30522-30527 NN denotes birch
T12988 30528-30536 NN denotes profilin
T12989 30537-30544 NN denotes peptide
T12990 30545-30546 -LRB- denotes (
T12991 30546-30548 NNP denotes BP
T12992 30548-30549 -RRB- denotes )
T12993 30549-30550 : denotes -
T12994 30550-30553 NN denotes tag
T12995 30554-30557 VBD denotes was
T12996 30558-30562 RB denotes also
T12997 30563-30571 VBN denotes prepared
T12998 30571-30572 . denotes .
T12999 30573-30583 NNP denotes Retroviral
T13000 30584-30593 NNS denotes particles
T13001 30594-30598 VBD denotes were
T13002 30599-30608 VBN denotes generated
T13003 30609-30611 IN denotes by
T13004 30612-30621 JJ denotes transient
T13005 30622-30634 NN denotes transfection
T13006 30635-30637 IN denotes of
T13007 30638-30642 JJ denotes 293T
T13008 30643-30648 NNS denotes cells
T13009 30649-30653 IN denotes with
T13010 30654-30658 NNP denotes pMIP
T13011 30658-30659 , denotes ,
T13012 30660-30669 NNP denotes pMIP-HOIP
T13013 30669-30670 , denotes ,
T13014 30671-30673 CC denotes or
T13015 30674-30683 NNP denotes pMIP-IKKγ
T13016 30684-30687 CC denotes and
T13017 30688-30691 DT denotes the
T13018 30692-30701 NN denotes packaging
T13019 30702-30708 NN denotes vector
T13020 30709-30716 NNP denotes pCL-Eco
T13021 30717-30718 NNP denotes [
T13022 30718-30720 CD denotes 32
T13023 30720-30721 NNP denotes ]
T13024 30721-30722 . denotes .
T13025 30723-30726 CD denotes Two
T13026 30727-30731 NNS denotes days
T13027 30732-30737 IN denotes after
T13028 30738-30750 NN denotes transfection
T13029 30750-30751 , denotes ,
T13030 30752-30759 NN denotes culture
T13031 30760-30772 NNS denotes supernatants
T13032 30773-30777 VBD denotes were
T13033 30778-30787 VBN denotes harvested
T13034 30788-30791 CC denotes and
T13035 30792-30800 VBN denotes filtered
T13036 30800-30801 . denotes .
T13037 30802-30805 CD denotes A20
T13038 30805-30807 CD denotes .2
T13039 30807-30808 NNP denotes J
T13040 30809-30811 CC denotes or
T13041 30812-30826 JJ denotes HOIP-deficient
T13042 30827-30832 NNS denotes cells
T13043 30833-30837 VBD denotes were
T13044 30838-30843 VBN denotes added
T13045 30844-30846 TO denotes to
T13046 30847-30854 JJ denotes 24-well
T13047 30855-30861 NNS denotes plates
T13048 30862-30863 -LRB- denotes (
T13049 30863-30866 CD denotes 2.5
T13050 30866-30867 CD denotes ×
T13051 30867-30870 CD denotes 105
T13052 30871-30881 NN denotes cells/well
T13053 30881-30882 -RRB- denotes )
T13054 30883-30887 IN denotes with
T13055 30888-30889 CD denotes 1
T13056 30890-30892 NN denotes ml
T13057 30893-30909 JJ denotes virus-containing
T13058 30910-30921 NN denotes supernatant
T13059 30922-30925 CC denotes and
T13060 30926-30927 CD denotes 8
T13061 30928-30930 NN denotes µg
T13062 30930-30931 NN denotes /
T13063 30931-30933 NN denotes ml
T13064 30934-30943 NN denotes polybrene
T13065 30943-30944 . denotes .
T13066 30945-30951 NNS denotes Plates
T13067 30952-30956 VBD denotes were
T13068 30957-30968 VBN denotes centrifuged
T13069 30969-30971 IN denotes at
T13070 30972-30975 CD denotes 500
T13071 30976-30977 SYM denotes x
T13072 30978-30979 FW denotes g
T13073 30980-30983 IN denotes for
T13074 30984-30985 CD denotes 2
T13075 30986-30989 NNS denotes hrs
T13076 30990-30992 IN denotes at
T13077 30993-30997 NN denotes room
T13078 30998-31009 NN denotes temperature
T13079 31009-31010 . denotes .
T13080 31011-31016 NNS denotes Cells
T13081 31017-31021 VBD denotes were
T13082 31022-31030 VBN denotes cultured
T13083 31031-31033 IN denotes in
T13084 31034-31035 NNP denotes B
T13085 31036-31040 NN denotes cell
T13086 31041-31048 NN denotes culture
T13087 31049-31055 NN denotes medium
T13088 31056-31059 IN denotes for
T13089 31060-31062 CD denotes 48
T13090 31063-31066 NNS denotes hrs
T13091 31066-31067 , denotes ,
T13092 31068-31073 IN denotes after
T13093 31074-31079 WDT denotes which
T13094 31080-31083 DT denotes the
T13095 31084-31094 VBN denotes transduced
T13096 31095-31100 NNS denotes cells
T13097 31101-31105 VBD denotes were
T13098 31106-31114 VBN denotes selected
T13099 31115-31117 IN denotes in
T13100 31118-31119 CD denotes 3
T13101 31120-31122 NN denotes µg
T13102 31122-31123 NN denotes /
T13103 31123-31125 NN denotes ml
T13104 31126-31135 NN denotes puromycin
T13105 31135-31136 . denotes .
T13347 31138-31142 CD denotes CD80
T13348 31143-31155 NN denotes upregulation
T13349 31156-31159 CC denotes and
T13350 31160-31164 NN denotes flow
T13351 31165-31174 NN denotes cytometry
T13352 31175-31180 NNP denotes Cells
T13353 31181-31182 -LRB- denotes (
T13354 31182-31183 CD denotes 5
T13355 31183-31184 CD denotes ×
T13356 31184-31187 CD denotes 104
T13357 31188-31190 IN denotes in
T13358 31191-31192 CD denotes 2
T13359 31193-31195 NN denotes ml
T13360 31195-31196 -RRB- denotes )
T13361 31197-31201 VBD denotes were
T13362 31202-31210 VBN denotes cultured
T13363 31211-31213 IN denotes in
T13364 31214-31221 JJ denotes 24-well
T13365 31222-31228 NNS denotes plates
T13366 31229-31232 IN denotes for
T13367 31233-31234 CD denotes 3
T13368 31235-31239 NNS denotes days
T13369 31240-31244 IN denotes with
T13370 31245-31246 CD denotes 5
T13371 31247-31249 NN denotes µg
T13372 31249-31250 NN denotes /
T13373 31250-31252 NN denotes ml
T13374 31253-31262 JJ denotes anti-CD40
T13375 31263-31265 CC denotes or
T13376 31266-31268 DT denotes an
T13377 31269-31276 NN denotes isotype
T13378 31277-31284 NN denotes control
T13379 31285-31293 NN denotes antibody
T13380 31293-31294 . denotes .
T13381 31295-31300 NNS denotes Cells
T13382 31301-31305 VBD denotes were
T13383 31306-31313 VBN denotes stained
T13384 31314-31317 IN denotes for
T13385 31318-31322 NN denotes flow
T13386 31323-31332 NN denotes cytometry
T13387 31333-31337 IN denotes with
T13388 31338-31352 NN denotes FITC-anti-CD80
T13389 31353-31355 CC denotes or
T13390 31356-31358 DT denotes an
T13391 31359-31370 JJ denotes appropriate
T13392 31371-31378 NN denotes control
T13393 31379-31387 NN denotes antibody
T13394 31388-31390 IN denotes as
T13395 31391-31400 VBN denotes described
T13396 31401-31402 NNP denotes [
T13397 31402-31404 CD denotes 28
T13398 31404-31405 NNP denotes ]
T13399 31405-31406 . denotes .
T13400 31407-31411 NNP denotes Data
T13401 31412-31420 VBD denotes acquired
T13402 31421-31425 IN denotes with
T13403 31426-31427 DT denotes a
T13404 31428-31435 JJ denotes FACScan
T13405 31436-31440 NN denotes flow
T13406 31441-31450 NN denotes cytometer
T13407 31451-31452 -LRB- denotes (
T13408 31452-31454 NNP denotes BD
T13409 31455-31466 NNPS denotes Biosciences
T13410 31466-31467 -RRB- denotes )
T13411 31468-31472 VBD denotes were
T13412 31473-31481 VBN denotes analyzed
T13413 31482-31486 IN denotes with
T13414 31487-31493 NNP denotes WinMDI
T13415 31494-31497 CD denotes 2.8
T13416 31498-31499 -LRB- denotes (
T13417 31499-31506 NNP denotes Scripps
T13418 31507-31515 NNP denotes Research
T13419 31516-31525 NNP denotes Institute
T13420 31525-31526 , denotes ,
T13421 31527-31530 NNP denotes San
T13422 31531-31536 NNP denotes Diego
T13423 31536-31537 , denotes ,
T13424 31538-31540 NNP denotes CA
T13425 31540-31541 -RRB- denotes )
T13426 31541-31542 . denotes .
T13569 31544-31547 NNP denotes GLε
T13570 31548-31558 NN denotes transcript
T13571 31559-31564 NN denotes assay
T13572 31565-31568 DT denotes The
T13573 31569-31583 JJ denotes CD40-simulated
T13574 31584-31594 NN denotes activation
T13575 31595-31597 IN denotes of
T13576 31598-31601 NNP denotes GLε
T13577 31602-31615 NN denotes transcription
T13578 31616-31619 VBD denotes was
T13579 31620-31629 VBN denotes evaluated
T13580 31630-31632 IN denotes as
T13581 31633-31643 RB denotes previously
T13582 31644-31652 VBN denotes reported
T13583 31653-31654 NNP denotes [
T13584 31654-31656 CD denotes 33
T13585 31656-31657 NNP denotes ]
T13586 31657-31658 . denotes .
T13587 31659-31666 RB denotes Briefly
T13588 31666-31667 , denotes ,
T13589 31668-31669 CD denotes 1
T13590 31669-31670 CD denotes ×
T13591 31670-31673 CD denotes 106
T13592 31674-31679 NNS denotes cells
T13593 31680-31684 VBD denotes were
T13594 31685-31695 VBN denotes stimulated
T13595 31696-31705 JJ denotes overnight
T13596 31706-31710 IN denotes with
T13597 31711-31720 JJ denotes anti-CD40
T13598 31721-31729 NN denotes antibody
T13599 31730-31731 -LRB- denotes (
T13600 31731-31733 CD denotes 10
T13601 31734-31736 NN denotes µg
T13602 31736-31737 NN denotes /
T13603 31737-31739 NN denotes ml
T13604 31739-31740 -RRB- denotes )
T13605 31741-31743 CC denotes or
T13606 31744-31746 DT denotes an
T13607 31747-31754 NN denotes isotype
T13608 31755-31762 NN denotes control
T13609 31763-31771 NN denotes antibody
T13610 31771-31772 , denotes ,
T13611 31773-31777 IN denotes with
T13612 31778-31780 CC denotes or
T13613 31781-31788 IN denotes without
T13614 31789-31792 CD denotes 500
T13615 31793-31797 NNP denotes U/ml
T13616 31798-31803 NN denotes mouse
T13617 31804-31808 NN denotes IL-4
T13618 31809-31810 -LRB- denotes (
T13619 31810-31812 NNP denotes BD
T13620 31813-31824 NNPS denotes Biosciences
T13621 31824-31825 -RRB- denotes )
T13622 31825-31826 . denotes .
T13623 31827-31830 NNP denotes RNA
T13624 31831-31834 VBD denotes was
T13625 31835-31843 VBN denotes isolated
T13626 31844-31849 VBG denotes using
T13627 31850-31856 NNP denotes Trizol
T13628 31857-31858 -LRB- denotes (
T13629 31858-31868 NNP denotes Invitrogen
T13630 31868-31869 -RRB- denotes )
T13631 31869-31870 , denotes ,
T13632 31871-31874 CC denotes and
T13633 31875-31894 JJ denotes reverse-transcribed
T13634 31895-31896 -LRB- denotes (
T13635 31896-31907 NNP denotes Superscript
T13636 31908-31911 NNP denotes III
T13637 31912-31915 NN denotes kit
T13638 31915-31916 , denotes ,
T13639 31917-31927 NNP denotes Invitrogen
T13640 31927-31928 -RRB- denotes )
T13641 31928-31929 . denotes .
T13642 31930-31942 JJ denotes Quantitative
T13643 31943-31946 NNP denotes PCR
T13644 31947-31950 IN denotes for
T13645 31951-31954 NNP denotes GLε
T13646 31955-31958 CC denotes and
T13647 31959-31964 NNP denotes Hprt1
T13648 31965-31968 VBD denotes was
T13649 31969-31978 VBN denotes performed
T13650 31979-31984 VBG denotes using
T13651 31985-31989 NNP denotes SYBR
T13652 31990-31995 NNP denotes GREEN
T13653 31996-32002 NN denotes master
T13654 32003-32006 NN denotes mix
T13655 32007-32008 -LRB- denotes (
T13656 32008-32015 NNP denotes Applied
T13657 32016-32026 NNPS denotes Biosystems
T13658 32026-32027 -RRB- denotes )
T13659 32027-32028 , denotes ,
T13660 32029-32032 CC denotes and
T13661 32033-32035 DT denotes an
T13662 32036-32043 NNP denotes Applied
T13663 32044-32054 NNPS denotes Biosystems
T13664 32055-32061 NNP denotes 7900HT
T13665 32062-32066 NNP denotes Fast
T13666 32067-32076 NNP denotes Real-Time
T13667 32077-32080 NNP denotes PCR
T13668 32081-32091 NN denotes instrument
T13669 32091-32092 . denotes .
T13670 32093-32103 NN denotes Expression
T13671 32104-32106 IN denotes of
T13672 32107-32110 NNP denotes GLε
T13673 32111-32113 IN denotes in
T13674 32114-32118 DT denotes each
T13675 32119-32125 NN denotes sample
T13676 32126-32129 VBD denotes was
T13677 32130-32140 VBN denotes normalized
T13678 32141-32143 TO denotes to
T13679 32144-32147 DT denotes the
T13680 32148-32158 NN denotes expression
T13681 32159-32161 IN denotes of
T13682 32162-32167 NNP denotes Hprt1
T13683 32167-32168 . denotes .
T13876 32170-32175 NN denotes NF-κB
T13877 32176-32179 CC denotes and
T13878 32180-32183 NNP denotes JNK
T13879 32184-32194 NN denotes activation
T13880 32195-32201 NN denotes assays
T13881 32202-32205 IN denotes For
T13882 32206-32216 NN denotes activation
T13883 32217-32219 IN denotes of
T13884 32220-32224 NNP denotes CD40
T13885 32225-32234 VBG denotes signaling
T13886 32234-32235 , denotes ,
T13887 32236-32237 CD denotes 5
T13888 32237-32238 NN denotes ×
T13889 32238-32241 CD denotes 104
T13890 32242-32245 CD denotes HI5
T13891 32246-32252 NN denotes insect
T13892 32253-32258 NNS denotes cells
T13893 32259-32261 CC denotes or
T13894 32262-32265 CD denotes HI5
T13895 32266-32272 JJ denotes insect
T13896 32273-32278 NNS denotes cells
T13897 32279-32289 VBG denotes expressing
T13898 32290-32295 NN denotes mouse
T13899 32296-32301 NNP denotes CD154
T13900 32302-32303 -LRB- denotes (
T13901 32303-32307 NNP denotes CD40
T13902 32308-32314 NN denotes ligand
T13903 32314-32315 -RRB- denotes )
T13904 32316-32320 VBD denotes were
T13905 32321-32326 VBN denotes added
T13906 32327-32329 TO denotes to
T13907 32330-32331 CD denotes 1
T13908 32331-32332 CD denotes ×
T13909 32332-32335 CD denotes 106
T13910 32336-32337 NNP denotes B
T13911 32338-32343 NNS denotes cells
T13912 32343-32344 . denotes .
T13913 32345-32350 NNS denotes Cells
T13914 32351-32355 VBD denotes were
T13915 32356-32367 VBN denotes centrifuged
T13916 32368-32371 IN denotes for
T13917 32372-32373 CD denotes 1
T13918 32374-32380 NN denotes minute
T13919 32381-32383 IN denotes at
T13920 32384-32387 CD denotes 400
T13921 32388-32389 SYM denotes x
T13922 32390-32391 FW denotes g
T13923 32392-32393 -LRB- denotes (
T13924 32393-32395 TO denotes to
T13925 32396-32403 VB denotes promote
T13926 32404-32411 NN denotes contact
T13927 32412-32419 IN denotes between
T13928 32420-32426 JJ denotes ligand
T13929 32427-32432 NNS denotes cells
T13930 32433-32436 CC denotes and
T13931 32437-32438 NNP denotes B
T13932 32439-32444 NNS denotes cells
T13933 32444-32445 -RRB- denotes )
T13934 32446-32450 RB denotes then
T13935 32451-32460 VBD denotes incubated
T13936 32461-32463 IN denotes at
T13937 32464-32466 CD denotes 37
T13938 32466-32467 CD denotes °
T13939 32467-32468 NNP denotes C
T13940 32469-32472 IN denotes for
T13941 32473-32478 NNS denotes times
T13942 32479-32488 VBD denotes indicated
T13943 32489-32491 IN denotes in
T13944 32492-32496 NNP denotes Fig.
T13945 32497-32498 CD denotes 4
T13946 32498-32499 . denotes .
T13947 32500-32509 VBG denotes Following
T13948 32510-32521 NN denotes stimulation
T13949 32521-32522 , denotes ,
T13950 32523-32528 NNS denotes cells
T13951 32529-32533 VBD denotes were
T13952 32534-32541 VBN denotes chilled
T13953 32542-32544 IN denotes on
T13954 32545-32548 NN denotes ice
T13955 32549-32552 IN denotes for
T13956 32553-32554 CD denotes 2
T13957 32555-32562 NNS denotes minutes
T13958 32562-32563 . denotes .
T13959 32564-32568 NN denotes Cell
T13960 32569-32576 NNS denotes pellets
T13961 32577-32581 VBD denotes were
T13962 32582-32591 VBN denotes dissolved
T13963 32592-32594 IN denotes in
T13964 32595-32597 NNP denotes 2X
T13965 32598-32606 NNP denotes SDS-PAGE
T13966 32607-32614 VBG denotes loading
T13967 32615-32621 NN denotes buffer
T13968 32621-32622 , denotes ,
T13969 32623-32632 VBN denotes sonicated
T13970 32632-32633 , denotes ,
T13971 32634-32637 CC denotes and
T13972 32638-32644 VBN denotes heated
T13973 32645-32648 IN denotes for
T13974 32649-32650 CD denotes 5
T13975 32651-32658 NNS denotes minutes
T13976 32659-32661 IN denotes at
T13977 32662-32664 CD denotes 95
T13978 32664-32665 CD denotes °
T13979 32665-32667 NNP denotes C.
T13980 32668-32673 NNP denotes Total
T13981 32674-32678 NN denotes cell
T13982 32679-32686 VBZ denotes lysates
T13983 32687-32688 -LRB- denotes (
T13984 32688-32689 CD denotes 1
T13985 32689-32690 CD denotes ×
T13986 32690-32693 CD denotes 105
T13987 32694-32698 NN denotes cell
T13988 32699-32710 NNS denotes equivalents
T13989 32711-32714 IN denotes per
T13990 32715-32719 NN denotes lane
T13991 32719-32720 -RRB- denotes )
T13992 32721-32725 VBD denotes were
T13993 32726-32738 VBN denotes fractionated
T13994 32739-32741 IN denotes by
T13995 32742-32750 NNP denotes SDS-PAGE
T13996 32751-32754 CC denotes and
T13997 32755-32762 VBD denotes blotted
T13998 32763-32765 TO denotes to
T13999 32766-32780 VB denotes polyvinylidine
T14000 32781-32789 NN denotes fluoride
T14001 32790-32791 -LRB- denotes (
T14002 32791-32795 NNP denotes PVDF
T14003 32795-32796 -RRB- denotes )
T14004 32797-32806 NNS denotes membranes
T14005 32806-32807 . denotes .
T14006 32808-32817 NNS denotes Membranes
T14007 32818-32822 VBD denotes were
T14008 32823-32829 VBN denotes probed
T14009 32830-32834 IN denotes with
T14010 32835-32845 NNS denotes antibodies
T14011 32846-32848 IN denotes as
T14012 32849-32858 VBN denotes indicated
T14013 32859-32861 IN denotes in
T14014 32862-32866 NNP denotes Fig.
T14015 32867-32868 CD denotes 4
T14016 32868-32869 . denotes .
T14017 32870-32886 JJ denotes Chemiluminescent
T14018 32887-32896 NN denotes detection
T14019 32897-32898 -LRB- denotes (
T14020 32898-32904 NNP denotes Pierce
T14021 32905-32918 NNP denotes Biotechnology
T14022 32918-32919 -RRB- denotes )
T14023 32920-32923 VBD denotes was
T14024 32924-32928 VBN denotes used
T14025 32929-32932 IN denotes for
T14026 32933-32936 DT denotes the
T14027 32937-32950 NN denotes visualization
T14028 32951-32953 IN denotes of
T14029 32954-32959 NNS denotes bands
T14030 32960-32962 IN denotes on
T14031 32963-32970 JJ denotes Western
T14032 32971-32976 NNS denotes blots
T14033 32976-32977 . denotes .
T14034 32978-32984 NNS denotes Images
T14035 32985-32987 IN denotes of
T14036 32988-32993 NNS denotes blots
T14037 32994-32998 VBD denotes were
T14038 32999-33007 VBN denotes recorded
T14039 33008-33012 IN denotes with
T14040 33013-33014 DT denotes a
T14041 33015-33024 JJ denotes low-light
T14042 33025-33032 NN denotes imaging
T14043 33033-33039 NN denotes system
T14044 33040-33041 -LRB- denotes (
T14045 33041-33048 CD denotes LAS4000
T14046 33048-33049 , denotes ,
T14047 33050-33054 NNP denotes Fuji
T14048 33055-33062 NNP denotes Medical
T14049 33063-33070 NNPS denotes Systems
T14050 33070-33071 -RRB- denotes )
T14051 33072-33075 CC denotes and
T14052 33076-33078 IN denotes on
T14053 33079-33084 NN denotes X-ray
T14054 33085-33089 NN denotes film
T14055 33089-33090 . denotes .
T14284 33092-33112 NNP denotes Immunoprecipitations
T14285 33113-33132 NNP denotes Immunoprecipitation
T14286 33133-33135 IN denotes of
T14287 33136-33140 NNP denotes CD40
T14288 33141-33144 VBD denotes was
T14289 33145-33154 VBN denotes performed
T14290 33155-33157 IN denotes by
T14291 33158-33167 VBN denotes activated
T14292 33168-33176 NN denotes receptor
T14293 33177-33184 NN denotes capture
T14294 33184-33185 , denotes ,
T14295 33186-33188 IN denotes as
T14296 33189-33199 RB denotes previously
T14297 33200-33209 VBN denotes described
T14298 33210-33211 NNP denotes [
T14299 33211-33212 CD denotes 6
T14300 33212-33213 NNP denotes ]
T14301 33213-33214 . denotes .
T14302 33215-33222 RB denotes Briefly
T14303 33222-33223 , denotes ,
T14304 33224-33225 CD denotes 3
T14305 33225-33226 NN denotes ×
T14306 33226-33229 CD denotes 107
T14307 33230-33235 NNS denotes cells
T14308 33236-33237 -LRB- denotes (
T14309 33237-33240 CD denotes A20
T14310 33240-33242 CD denotes .2
T14311 33242-33243 NNP denotes J
T14312 33244-33247 CC denotes and
T14313 33248-33259 NNS denotes derivatives
T14314 33259-33260 -RRB- denotes )
T14315 33261-33265 VBD denotes were
T14316 33266-33275 VBN denotes incubated
T14317 33276-33279 IN denotes for
T14318 33280-33282 CD denotes 60
T14319 33283-33290 NNS denotes minutes
T14320 33291-33293 IN denotes at
T14321 33294-33298 NN denotes room
T14322 33299-33310 NN denotes temperature
T14323 33311-33315 IN denotes with
T14324 33316-33318 CD denotes 10
T14325 33319-33321 NN denotes µl
T14326 33322-33330 JJ denotes magnetic
T14327 33331-33338 NN denotes protein
T14328 33339-33340 NNP denotes G
T14329 33341-33346 NNS denotes beads
T14330 33347-33348 -LRB- denotes (
T14331 33348-33353 NNP denotes Dynal
T14332 33353-33354 -RRB- denotes )
T14333 33355-33365 JJ denotes pre-coated
T14334 33366-33370 IN denotes with
T14335 33371-33373 CD denotes 10
T14336 33374-33376 NN denotes µg
T14337 33377-33381 NN denotes goat
T14338 33382-33390 JJ denotes anti-rat
T14339 33391-33394 NNP denotes IgG
T14340 33395-33396 -LRB- denotes (
T14341 33396-33403 NNP denotes Jackson
T14342 33403-33404 -RRB- denotes )
T14343 33404-33405 , denotes ,
T14344 33406-33409 CC denotes and
T14345 33410-33412 CD denotes 10
T14346 33413-33415 NN denotes µg
T14347 33416-33425 NN denotes anti-CD40
T14348 33426-33427 -LRB- denotes (
T14349 33427-33431 NNP denotes 1C10
T14350 33431-33432 -RRB- denotes )
T14351 33433-33435 CC denotes or
T14352 33436-33438 DT denotes an
T14353 33439-33446 NN denotes isotype
T14354 33447-33454 NN denotes control
T14355 33455-33463 NN denotes antibody
T14356 33464-33465 -LRB- denotes (
T14357 33465-33470 NNP denotes mAb72
T14358 33470-33471 -RRB- denotes )
T14359 33471-33472 . denotes .
T14360 33473-33484 NNS denotes Cells/beads
T14361 33485-33489 VBD denotes were
T14362 33490-33494 RB denotes then
T14363 33495-33503 VBN denotes pelleted
T14364 33504-33506 IN denotes by
T14365 33507-33521 NN denotes centrifugation
T14366 33522-33525 CC denotes and
T14367 33526-33531 VBN denotes lysed
T14368 33532-33534 IN denotes in
T14369 33535-33541 NN denotes buffer
T14370 33542-33552 VBG denotes containing
T14371 33553-33554 CD denotes 1
T14372 33554-33555 NN denotes %
T14373 33556-33562 NNP denotes Triton
T14374 33563-33567 NNP denotes X100
T14375 33567-33568 . denotes .
T14376 33569-33574 NNS denotes Beads
T14377 33575-33579 VBD denotes were
T14378 33580-33586 VBN denotes washed
T14379 33587-33591 IN denotes with
T14380 33592-33597 NN denotes lysis
T14381 33598-33604 NN denotes buffer
T14382 33605-33607 TO denotes to
T14383 33608-33614 VB denotes remove
T14384 33615-33622 JJ denotes unbound
T14385 33623-33631 NN denotes material
T14386 33631-33632 . denotes .
T14387 33633-33635 IN denotes In
T14388 33636-33640 DT denotes some
T14389 33641-33652 NNS denotes experiments
T14390 33652-33653 , denotes ,
T14391 33654-33662 NN denotes material
T14392 33663-33673 VBN denotes associated
T14393 33674-33678 IN denotes with
T14394 33679-33682 DT denotes the
T14395 33683-33688 NNS denotes beads
T14396 33689-33692 VBD denotes was
T14397 33693-33709 VBN denotes dephosphorylated
T14398 33710-33714 IN denotes with
T14399 33715-33721 JJ denotes lambda
T14400 33722-33733 NN denotes phosphatase
T14401 33734-33735 -LRB- denotes (
T14402 33735-33738 NNP denotes New
T14403 33739-33746 NNP denotes England
T14404 33747-33754 NNP denotes BioLabs
T14405 33754-33755 -RRB- denotes )
T14406 33756-33758 IN denotes as
T14407 33759-33762 IN denotes per
T14408 33763-33775 NN denotes manufacturer
T14409 33775-33777 POS denotes 's
T14410 33778-33790 NNS denotes instructions
T14411 33790-33791 . denotes .
T14412 33792-33797 NNS denotes Beads
T14413 33798-33802 VBD denotes were
T14414 33803-33814 VBN denotes resuspended
T14415 33815-33817 IN denotes in
T14416 33818-33820 NNP denotes 2X
T14417 33821-33829 NNP denotes SDS-PAGE
T14418 33830-33836 NN denotes sample
T14419 33837-33843 NN denotes buffer
T14420 33844-33847 CC denotes and
T14421 33848-33854 VBN denotes heated
T14422 33855-33858 IN denotes for
T14423 33859-33860 CD denotes 5
T14424 33861-33868 NNS denotes minutes
T14425 33869-33871 IN denotes at
T14426 33872-33874 CD denotes 95
T14427 33874-33875 CD denotes °
T14428 33875-33877 NNP denotes C.
T14429 33878-33886 NNP denotes Material
T14430 33887-33893 VBD denotes eluted
T14431 33894-33898 IN denotes from
T14432 33899-33902 DT denotes the
T14433 33903-33908 NNS denotes beads
T14434 33909-33912 VBD denotes was
T14435 33913-33925 VBN denotes fractionated
T14436 33926-33928 IN denotes by
T14437 33929-33937 NNP denotes SDS-PAGE
T14438 33938-33941 CC denotes and
T14439 33942-33953 VBN denotes transferred
T14440 33954-33956 TO denotes to
T14441 33957-33961 NNP denotes PVDF
T14442 33962-33971 NNS denotes membranes
T14443 33972-33975 IN denotes for
T14444 33976-33983 JJ denotes Western
T14445 33984-33992 VBG denotes blotting
T14446 33992-33993 . denotes .
T14447 33994-33996 IN denotes In
T14448 33997-34001 DT denotes some
T14449 34002-34013 NNS denotes experiments
T14450 34013-34014 , denotes ,
T14451 34015-34020 NNS denotes cells
T14452 34021-34025 VBD denotes were
T14453 34026-34034 VBN denotes cultured
T14454 34035-34038 IN denotes for
T14455 34039-34040 CD denotes 6
T14456 34041-34044 NNS denotes hrs
T14457 34045-34049 IN denotes with
T14458 34050-34052 CD denotes 25
T14459 34053-34055 NNP denotes µM
T14460 34056-34075 JJ denotes antennapedia-linked
T14461 34076-34083 NNP denotes SMAC-N7
T14462 34084-34091 NN denotes peptide
T14463 34092-34093 -LRB- denotes (
T14464 34093-34103 NNP denotes Calbiochem
T14465 34103-34104 -RRB- denotes )
T14466 34105-34107 CC denotes or
T14467 34108-34110 DT denotes an
T14468 34111-34122 JJ denotes appropriate
T14469 34123-34129 NN denotes volume
T14470 34130-34132 IN denotes of
T14471 34133-34136 DT denotes the
T14472 34137-34144 JJ denotes solvent
T14473 34145-34149 VBN denotes used
T14474 34150-34153 IN denotes for
T14475 34154-34157 DT denotes the
T14476 34158-34165 NN denotes peptide
T14477 34166-34167 -LRB- denotes (
T14478 34167-34171 NNP denotes DMSO
T14479 34171-34172 -RRB- denotes )
T14480 34172-34173 . denotes .
T14481 34174-34179 IN denotes After
T14482 34180-34190 NN denotes incubation
T14483 34190-34191 , denotes ,
T14484 34192-34197 NNS denotes cells
T14485 34198-34199 -LRB- denotes (
T14486 34199-34201 IN denotes in
T14487 34202-34209 NN denotes peptide
T14488 34209-34210 : denotes -
T14489 34211-34213 CC denotes or
T14490 34214-34229 JJ denotes DMSO-containing
T14491 34230-34236 NN denotes medium
T14492 34236-34237 -RRB- denotes )
T14493 34238-34242 VBD denotes were
T14494 34243-34253 VBN denotes stimulated
T14495 34254-34258 IN denotes with
T14496 34259-34274 JJ denotes antibody-coated
T14497 34275-34280 NNS denotes beads
T14498 34281-34283 IN denotes as
T14499 34284-34292 VBN denotes outlined
T14500 34293-34298 IN denotes above
T14501 34298-34299 . denotes .
R100 T178 T177 amod critical,signals
R1000 T1382 T1383 det These,proteins
R1001 T1383 T1384 nsubj proteins,function
R1002 T1384 T1384 ROOT function,function
R1003 T1385 T1384 prep in,function
R1004 T1386 T1385 pobj part,in
R1005 T1387 T1384 prep as,function
R1006 T1388 T1389 compound adaptor,molecules
R1007 T1389 T1387 pobj molecules,as
R1008 T1390 T1389 punct ",",molecules
R1009 T1391 T1384 advcl binding,function
R10097 T12911 T12912 compound Retroviral,transduction
R10098 T12912 T12912 ROOT transduction,transduction
R10099 T12913 T12915 det A,vector
R101 T179 T178 prep for,critical
R1010 T1392 T1391 prep to,binding
R10100 T12914 T12915 amod retroviral,vector
R10101 T12915 T12920 nsubjpass vector,used
R10102 T12916 T12917 punct (,pMIP
R10103 T12917 T12915 appos pMIP,vector
R10104 T12918 T12917 punct ),pMIP
R10105 T12919 T12920 auxpass was,used
R10106 T12920 T12920 ROOT used,used
R10107 T12921 T12920 prep for,used
R10108 T12922 T12924 det the,transduction
R10109 T12923 T12924 amod stable,transduction
R1011 T1393 T1395 det the,tail
R10110 T12924 T12921 pobj transduction,for
R10111 T12925 T12924 prep of,transduction
R10112 T12926 T12925 pobj cells,of
R10113 T12927 T12924 prep with,transduction
R10114 T12928 T12932 nmod HOIP,constructs
R10115 T12929 T12928 cc and,HOIP
R10116 T12930 T12931 compound IKKγ,cDNA
R10117 T12931 T12928 conj cDNA,HOIP
R10118 T12932 T12927 pobj constructs,with
R10119 T12933 T12920 punct .,used
R1012 T1394 T1395 amod cytoplasmic,tail
R10120 T12934 T12936 nsubjpass pMIP,constructed
R10121 T12935 T12936 auxpass was,constructed
R10122 T12936 T12936 ROOT constructed,constructed
R10123 T12937 T12936 prep by,constructed
R10124 T12938 T12937 pcomp replacing,by
R10125 T12939 T12940 det the,IRES
R10126 T12940 T12938 dobj IRES,replacing
R10127 T12941 T12940 cc and,IRES
R10128 T12942 T12940 conj GFP,IRES
R10129 T12943 T12944 amod encoding,sequences
R1013 T1395 T1392 pobj tail,to
R10130 T12944 T12938 dobj sequences,replacing
R10131 T12945 T12938 prep in,replacing
R10132 T12946 T12947 compound pMIG,[
R10133 T12947 T12945 pobj [,in
R10134 T12948 T12949 nummod 31,]
R10135 T12949 T12947 appos ],[
R10136 T12950 T12949 prep with,]
R10137 T12951 T12952 det the,IRES
R10138 T12952 T12956 nmod IRES,gene
R10139 T12953 T12952 cc and,IRES
R1014 T1396 T1395 prep of,tail
R10140 T12954 T12956 amod puromycin,gene
R10141 T12955 T12956 compound resistance,gene
R10142 T12956 T12950 pobj gene,with
R10143 T12957 T12956 prep from,gene
R10144 T12958 T12957 pobj pIRESpuro2,from
R10145 T12959 T12958 punct (,pIRESpuro2
R10146 T12960 T12958 appos Clontech,pIRESpuro2
R10147 T12961 T12958 punct ),pIRESpuro2
R10148 T12962 T12936 punct .,constructed
R10149 T12963 T12964 amod FLAG-tagged,mouse
R1015 T1397 T1396 pobj CD40,of
R10150 T12964 T12966 compound mouse,cDNA
R10151 T12965 T12966 compound HOIP,cDNA
R10152 T12966 T12968 nsubjpass cDNA,inserted
R10153 T12967 T12968 auxpass was,inserted
R10154 T12968 T12968 ROOT inserted,inserted
R10155 T12969 T12968 prep into,inserted
R10156 T12970 T12973 det the,site
R10157 T12971 T12973 amod multiple,site
R10158 T12972 T12973 compound cloning,site
R10159 T12973 T12969 pobj site,into
R1016 T1398 T1395 cc and,tail
R10160 T12974 T12973 prep of,site
R10161 T12975 T12974 pobj pMIP,of
R10162 T12976 T12968 punct .,inserted
R10163 T12977 T12978 det A,pMIP
R10164 T12978 T12979 nsubj pMIP,construct
R10165 T12979 T12979 ROOT construct,construct
R10166 T12980 T12981 amod encoding,mouse
R10167 T12981 T12982 compound mouse,IKKγ
R10168 T12982 T12979 dobj IKKγ,construct
R10169 T12983 T12979 prep with,construct
R1017 T1399 T1384 advcl recruiting,function
R10170 T12984 T12989 det an,peptide
R10171 T12985 T12987 amod amino,birch
R10172 T12986 T12987 compound terminal,birch
R10173 T12987 T12989 compound birch,peptide
R10174 T12988 T12989 compound profilin,peptide
R10175 T12989 T12983 pobj peptide,with
R10176 T12990 T12991 punct (,BP
R10177 T12991 T12989 appos BP,peptide
R10178 T12992 T12979 punct ),construct
R10179 T12993 T12994 punct -,tag
R1018 T1400 T1401 amod other,proteins
R10180 T12994 T12997 nsubjpass tag,prepared
R10181 T12995 T12997 auxpass was,prepared
R10182 T12996 T12997 advmod also,prepared
R10183 T12997 T12979 conj prepared,construct
R10184 T12998 T12997 punct .,prepared
R10185 T12999 T13000 compound Retroviral,particles
R10186 T13000 T13002 nsubjpass particles,generated
R10187 T13001 T13002 auxpass were,generated
R10188 T13002 T13002 ROOT generated,generated
R10189 T13003 T13002 agent by,generated
R1019 T1401 T1399 dobj proteins,recruiting
R10190 T13004 T13005 amod transient,transfection
R10191 T13005 T13003 pobj transfection,by
R10192 T13006 T13005 prep of,transfection
R10193 T13007 T13008 amod 293T,cells
R10194 T13008 T13006 pobj cells,of
R10195 T13009 T13005 prep with,transfection
R10196 T13010 T13009 pobj pMIP,with
R10197 T13011 T13010 punct ",",pMIP
R10198 T13012 T13010 conj pMIP-HOIP,pMIP
R10199 T13013 T13012 punct ",",pMIP-HOIP
R102 T180 T179 pobj anti-microbial,for
R1020 T1402 T1399 prep to,recruiting
R10200 T13014 T13012 cc or,pMIP-HOIP
R10201 T13015 T13012 conj pMIP-IKKγ,pMIP-HOIP
R10202 T13016 T13015 cc and,pMIP-IKKγ
R10203 T13017 T13019 det the,vector
R10204 T13018 T13019 compound packaging,vector
R10205 T13019 T13023 nmod vector,]
R10206 T13020 T13021 compound pCL-Eco,[
R10207 T13021 T13019 appos [,vector
R10208 T13022 T13023 nummod 32,]
R10209 T13023 T13015 conj ],pMIP-IKKγ
R1021 T1403 T1405 det the,complex
R10210 T13024 T13002 punct .,generated
R10211 T13025 T13026 nummod Two,days
R10212 T13026 T13027 npadvmod days,after
R10213 T13027 T13033 prep after,harvested
R10214 T13028 T13027 pobj transfection,after
R10215 T13029 T13033 punct ",",harvested
R10216 T13030 T13031 compound culture,supernatants
R10217 T13031 T13033 nsubjpass supernatants,harvested
R10218 T13032 T13033 auxpass were,harvested
R10219 T13033 T13033 ROOT harvested,harvested
R1022 T1404 T1405 compound receptor-associated,complex
R10220 T13034 T13033 cc and,harvested
R10221 T13035 T13033 conj filtered,harvested
R10222 T13036 T13033 punct .,harvested
R10223 T13037 T13039 nummod A20,J
R10224 T13038 T13039 nummod .2,J
R10225 T13039 T13042 nmod J,cells
R10226 T13040 T13039 cc or,J
R10227 T13041 T13039 conj HOIP-deficient,J
R10228 T13042 T13044 nsubjpass cells,added
R10229 T13043 T13044 auxpass were,added
R1023 T1405 T1402 pobj complex,to
R10230 T13044 T13044 ROOT added,added
R10231 T13045 T13044 prep to,added
R10232 T13046 T13047 amod 24-well,plates
R10233 T13047 T13045 pobj plates,to
R10234 T13048 T13047 punct (,plates
R10235 T13049 T13052 nummod 2.5,cells/well
R10236 T13050 T13052 nummod ×,cells/well
R10237 T13051 T13052 nummod 105,cells/well
R10238 T13052 T13047 appos cells/well,plates
R10239 T13053 T13047 punct ),plates
R1024 T1406 T1384 punct .,function
R10240 T13054 T13044 prep with,added
R10241 T13055 T13056 nummod 1,ml
R10242 T13056 T13058 nmod ml,supernatant
R10243 T13057 T13058 amod virus-containing,supernatant
R10244 T13058 T13054 pobj supernatant,with
R10245 T13059 T13058 cc and,supernatant
R10246 T13060 T13061 nummod 8,µg
R10247 T13061 T13064 nmod µg,polybrene
R10248 T13062 T13064 nmod /,polybrene
R10249 T13063 T13064 compound ml,polybrene
R1025 T1407 T1412 nsubj Some,function
R10250 T13064 T13058 conj polybrene,supernatant
R10251 T13065 T13044 punct .,added
R10252 T13066 T13068 nsubjpass Plates,centrifuged
R10253 T13067 T13068 auxpass were,centrifuged
R10254 T13068 T13068 ROOT centrifuged,centrifuged
R10255 T13069 T13068 prep at,centrifuged
R10256 T13070 T13072 nummod 500,g
R10257 T13071 T13072 punct x,g
R10258 T13072 T13069 pobj g,at
R10259 T13073 T13072 prep for,g
R1026 T1408 T1407 prep of,Some
R10260 T13074 T13075 nummod 2,hrs
R10261 T13075 T13073 pobj hrs,for
R10262 T13076 T13072 prep at,g
R10263 T13077 T13078 compound room,temperature
R10264 T13078 T13076 pobj temperature,at
R10265 T13079 T13068 punct .,centrifuged
R10266 T13080 T13082 nsubjpass Cells,cultured
R10267 T13081 T13082 auxpass were,cultured
R10268 T13082 T13082 ROOT cultured,cultured
R10269 T13083 T13082 prep in,cultured
R1027 T1409 T1410 det the,TRAFs
R10270 T13084 T13086 compound B,culture
R10271 T13085 T13086 compound cell,culture
R10272 T13086 T13087 compound culture,medium
R10273 T13087 T13083 pobj medium,in
R10274 T13088 T13082 prep for,cultured
R10275 T13089 T13090 nummod 48,hrs
R10276 T13090 T13088 pobj hrs,for
R10277 T13091 T13082 punct ",",cultured
R10278 T13092 T13098 prep after,selected
R10279 T13093 T13092 pobj which,after
R1028 T1410 T1408 pobj TRAFs,of
R10280 T13094 T13096 det the,cells
R10281 T13095 T13096 amod transduced,cells
R10282 T13096 T13098 nsubjpass cells,selected
R10283 T13097 T13098 auxpass were,selected
R10284 T13098 T13082 advcl selected,cultured
R10285 T13099 T13098 prep in,selected
R10286 T13100 T13101 nummod 3,µg
R10287 T13101 T13104 nmod µg,puromycin
R10288 T13102 T13104 nmod /,puromycin
R10289 T13103 T13104 compound ml,puromycin
R1029 T1411 T1412 advmod also,function
R10290 T13104 T13099 pobj puromycin,in
R10291 T13105 T13082 punct .,cultured
R103 T181 T180 cc and,anti-microbial
R1030 T1412 T1412 ROOT function,function
R1031 T1413 T1412 prep as,function
R1032 T1414 T1416 nummod E3,ligases
R1033 T1415 T1416 compound ubiquitin,ligases
R1034 T1416 T1413 pobj ligases,as
R1035 T1417 T1412 punct ",",function
R1036 T1418 T1412 cc and,function
R1037 T1419 T1421 det this,activity
R1038 T1420 T1421 amod enzymatic,activity
R1039 T1421 T1423 nsubj activity,contribute
R104 T182 T185 nmod T,responses
R1040 T1422 T1423 aux may,contribute
R1041 T1423 T1412 conj contribute,function
R1042 T1424 T1425 aux to,signal
R1043 T1425 T1423 xcomp signal,contribute
R1044 T1426 T1425 dobj propagation,signal
R1045 T1427 T1426 cc and,propagation
R1046 T1428 T1426 conj regulation,propagation
R1047 T1429 T1412 punct .,function
R1048 T1430 T1441 prep Among,function
R10480 T13347 T13348 nummod CD80,upregulation
R10481 T13348 T13361 npadvmod upregulation,were
R10482 T13349 T13348 cc and,upregulation
R10483 T13350 T13352 compound flow,Cells
R10484 T13351 T13352 compound cytometry,Cells
R10485 T13352 T13348 conj Cells,upregulation
R10486 T13353 T13354 punct (,5
R10487 T13354 T13352 nummod 5,Cells
R10488 T13355 T13356 nummod ×,104
R10489 T13356 T13348 appos 104,upregulation
R1049 T1431 T1433 det the,TRAFs
R10490 T13357 T13356 prep in,104
R10491 T13358 T13359 nummod 2,ml
R10492 T13359 T13357 pobj ml,in
R10493 T13360 T13348 punct ),upregulation
R10494 T13361 T13361 ROOT were,were
R10495 T13362 T13361 acomp cultured,were
R10496 T13363 T13362 prep in,cultured
R10497 T13364 T13365 amod 24-well,plates
R10498 T13365 T13363 pobj plates,in
R10499 T13366 T13361 prep for,were
R105 T183 T185 amod cell-mediated,responses
R1050 T1432 T1433 amod multiple,TRAFs
R10500 T13367 T13368 nummod 3,days
R10501 T13368 T13366 pobj days,for
R10502 T13369 T13361 prep with,were
R10503 T13370 T13371 nummod 5,µg
R10504 T13371 T13374 amod µg,anti-CD40
R10505 T13372 T13374 nmod /,anti-CD40
R10506 T13373 T13374 compound ml,anti-CD40
R10507 T13374 T13369 pobj anti-CD40,with
R10508 T13375 T13374 cc or,anti-CD40
R10509 T13376 T13379 det an,antibody
R1051 T1433 T1430 pobj TRAFs,Among
R10510 T13377 T13379 amod isotype,antibody
R10511 T13378 T13379 compound control,antibody
R10512 T13379 T13374 conj antibody,anti-CD40
R10513 T13380 T13361 punct .,were
R10514 T13381 T13383 nsubjpass Cells,stained
R10515 T13382 T13383 auxpass were,stained
R10516 T13383 T13383 ROOT stained,stained
R10517 T13384 T13383 prep for,stained
R10518 T13385 T13384 pobj flow,for
R10519 T13386 T13383 conj cytometry,stained
R1052 T1434 T1435 nsubj that,associate
R10520 T13387 T13386 prep with,cytometry
R10521 T13388 T13387 pobj FITC-anti-CD80,with
R10522 T13389 T13388 cc or,FITC-anti-CD80
R10523 T13390 T13393 det an,antibody
R10524 T13391 T13393 amod appropriate,antibody
R10525 T13392 T13393 compound control,antibody
R10526 T13393 T13388 conj antibody,FITC-anti-CD80
R10527 T13394 T13395 mark as,described
R10528 T13395 T13383 advcl described,stained
R10529 T13396 T13398 nmod [,]
R1053 T1435 T1433 relcl associate,TRAFs
R10530 T13397 T13398 nummod 28,]
R10531 T13398 T13395 dobj ],described
R10532 T13399 T13383 punct .,stained
R10533 T13400 T13401 nsubj Data,acquired
R10534 T13401 T13401 ROOT acquired,acquired
R10535 T13402 T13401 prep with,acquired
R10536 T13403 T13405 det a,flow
R10537 T13404 T13405 amod FACScan,flow
R10538 T13405 T13406 compound flow,cytometer
R10539 T13406 T13402 pobj cytometer,with
R1054 T1436 T1435 prep with,associate
R10540 T13407 T13409 punct (,Biosciences
R10541 T13408 T13409 compound BD,Biosciences
R10542 T13409 T13406 appos Biosciences,cytometer
R10543 T13410 T13401 punct ),acquired
R10544 T13411 T13412 auxpass were,analyzed
R10545 T13412 T13401 conj analyzed,acquired
R10546 T13413 T13412 prep with,analyzed
R10547 T13414 T13413 pobj WinMDI,with
R10548 T13415 T13414 nummod 2.8,WinMDI
R10549 T13416 T13419 punct (,Institute
R1055 T1437 T1436 pobj CD40,with
R10550 T13417 T13419 compound Scripps,Institute
R10551 T13418 T13419 compound Research,Institute
R10552 T13419 T13419 ROOT Institute,Institute
R10553 T13420 T13419 punct ",",Institute
R10554 T13421 T13422 compound San,Diego
R10555 T13422 T13419 npadvmod Diego,Institute
R10556 T13423 T13422 punct ",",Diego
R10557 T13424 T13422 appos CA,Diego
R10558 T13425 T13419 punct ),Institute
R10559 T13426 T13419 punct .,Institute
R1056 T1438 T1441 punct ",",function
R1057 T1439 T1441 nsubj TRAF3,function
R1058 T1440 T1441 aux can,function
R1059 T1441 T1441 ROOT function,function
R106 T184 T185 amod immune,responses
R1060 T1442 T1441 prep as,function
R1061 T1443 T1445 det a,regulator
R1062 T1444 T1445 amod negative,regulator
R1063 T1445 T1442 pobj regulator,as
R1064 T1446 T1445 prep of,regulator
R1065 T1447 T1446 pcomp signaling,of
R10653 T13569 T13579 nsubjpass GLε,evaluated
R10654 T13570 T13571 compound transcript,assay
R10655 T13571 T13569 dobj assay,GLε
R10656 T13572 T13574 det The,activation
R10657 T13573 T13574 amod CD40-simulated,activation
R10658 T13574 T13579 nsubjpass activation,evaluated
R10659 T13575 T13574 prep of,activation
R1066 T1448 T1441 punct ",",function
R10660 T13576 T13577 compound GLε,transcription
R10661 T13577 T13575 pobj transcription,of
R10662 T13578 T13579 auxpass was,evaluated
R10663 T13579 T13579 ROOT evaluated,evaluated
R10664 T13580 T13582 mark as,reported
R10665 T13581 T13582 advmod previously,reported
R10666 T13582 T13579 advcl reported,evaluated
R10667 T13583 T13585 nmod [,]
R10668 T13584 T13585 nummod 33,]
R10669 T13585 T13582 nsubj ],reported
R1067 T1449 T1453 mark while,promote
R10670 T13586 T13579 punct .,evaluated
R10671 T13587 T13594 advmod Briefly,stimulated
R10672 T13588 T13594 punct ",",stimulated
R10673 T13589 T13592 nummod 1,cells
R10674 T13590 T13592 nummod ×,cells
R10675 T13591 T13592 nummod 106,cells
R10676 T13592 T13594 nsubjpass cells,stimulated
R10677 T13593 T13594 auxpass were,stimulated
R10678 T13594 T13594 ROOT stimulated,stimulated
R10679 T13595 T13594 advmod overnight,stimulated
R1068 T1450 T1453 nsubj TRAF2,promote
R10680 T13596 T13594 prep with,stimulated
R10681 T13597 T13598 amod anti-CD40,antibody
R10682 T13598 T13596 pobj antibody,with
R10683 T13599 T13598 punct (,antibody
R10684 T13600 T13601 nummod 10,µg
R10685 T13601 T13603 compound µg,ml
R10686 T13602 T13603 compound /,ml
R10687 T13603 T13598 appos ml,antibody
R10688 T13604 T13598 punct ),antibody
R10689 T13605 T13598 cc or,antibody
R1069 T1451 T1450 cc and,TRAF2
R10690 T13606 T13609 det an,antibody
R10691 T13607 T13609 compound isotype,antibody
R10692 T13608 T13609 compound control,antibody
R10693 T13609 T13598 conj antibody,antibody
R10694 T13610 T13596 punct ",",with
R10695 T13611 T13596 conj with,with
R10696 T13612 T13611 cc or,with
R10697 T13613 T13611 conj without,with
R10698 T13614 T13617 nummod 500,IL-4
R10699 T13615 T13617 compound U/ml,IL-4
R107 T185 T180 conj responses,anti-microbial
R1070 T1452 T1450 conj TRAF6,TRAF2
R10700 T13616 T13617 compound mouse,IL-4
R10701 T13617 T13613 pobj IL-4,without
R10702 T13618 T13620 punct (,Biosciences
R10703 T13619 T13620 compound BD,Biosciences
R10704 T13620 T13617 appos Biosciences,IL-4
R10705 T13621 T13620 punct ),Biosciences
R10706 T13622 T13594 punct .,stimulated
R10707 T13623 T13625 nsubjpass RNA,isolated
R10708 T13624 T13625 auxpass was,isolated
R10709 T13625 T13625 ROOT isolated,isolated
R1071 T1453 T1441 advcl promote,function
R10710 T13626 T13625 advcl using,isolated
R10711 T13627 T13626 dobj Trizol,using
R10712 T13628 T13629 punct (,Invitrogen
R10713 T13629 T13627 appos Invitrogen,Trizol
R10714 T13630 T13629 punct ),Invitrogen
R10715 T13631 T13627 punct ",",Trizol
R10716 T13632 T13627 cc and,Trizol
R10717 T13633 T13627 conj reverse-transcribed,Trizol
R10718 T13634 T13633 punct (,reverse-transcribed
R10719 T13635 T13636 compound Superscript,III
R1072 T1454 T1455 det the,activation
R10720 T13636 T13637 compound III,kit
R10721 T13637 T13633 appos kit,reverse-transcribed
R10722 T13638 T13637 punct ",",kit
R10723 T13639 T13637 appos Invitrogen,kit
R10724 T13640 T13633 punct ),reverse-transcribed
R10725 T13641 T13625 punct .,isolated
R10726 T13642 T13643 compound Quantitative,PCR
R10727 T13643 T13649 nsubjpass PCR,performed
R10728 T13644 T13643 prep for,PCR
R10729 T13645 T13644 pobj GLε,for
R1073 T1455 T1453 dobj activation,promote
R10730 T13646 T13645 cc and,GLε
R10731 T13647 T13645 conj Hprt1,GLε
R10732 T13648 T13649 auxpass was,performed
R10733 T13649 T13649 ROOT performed,performed
R10734 T13650 T13649 advcl using,performed
R10735 T13651 T13654 compound SYBR,mix
R10736 T13652 T13654 compound GREEN,mix
R10737 T13653 T13654 compound master,mix
R10738 T13654 T13650 dobj mix,using
R10739 T13655 T13657 punct (,Biosystems
R1074 T1456 T1455 prep of,activation
R10740 T13656 T13657 compound Applied,Biosystems
R10741 T13657 T13654 appos Biosystems,mix
R10742 T13658 T13654 punct ),mix
R10743 T13659 T13649 punct ",",performed
R10744 T13660 T13649 cc and,performed
R10745 T13661 T13668 det an,instrument
R10746 T13662 T13665 compound Applied,Fast
R10747 T13663 T13665 compound Biosystems,Fast
R10748 T13664 T13665 compound 7900HT,Fast
R10749 T13665 T13667 compound Fast,PCR
R1075 T1457 T1459 amod downstream,pathways
R10750 T13666 T13667 compound Real-Time,PCR
R10751 T13667 T13668 compound PCR,instrument
R10752 T13668 T13649 conj instrument,performed
R10753 T13669 T13649 punct .,performed
R10754 T13670 T13676 nsubj Expression,was
R10755 T13671 T13670 prep of,Expression
R10756 T13672 T13671 pobj GLε,of
R10757 T13673 T13670 prep in,Expression
R10758 T13674 T13675 det each,sample
R10759 T13675 T13673 pobj sample,in
R1076 T1458 T1459 compound signaling,pathways
R10760 T13676 T13676 ROOT was,was
R10761 T13677 T13676 acomp normalized,was
R10762 T13678 T13677 prep to,normalized
R10763 T13679 T13680 det the,expression
R10764 T13680 T13678 pobj expression,to
R10765 T13681 T13680 prep of,expression
R10766 T13682 T13681 pobj Hprt1,of
R10767 T13683 T13676 punct .,was
R1077 T1459 T1456 pobj pathways,of
R1078 T1460 T1462 nmod [,]
R1079 T1461 T1462 nummod 4,]
R108 T186 T185 punct ",",responses
R1080 T1462 T1455 appos ],activation
R1081 T1463 T1441 punct .,function
R1082 T1464 T1466 nsubj We,demonstrated
R1083 T1465 T1466 advmod recently,demonstrated
R1084 T1466 T1466 ROOT demonstrated,demonstrated
R1085 T1467 T1487 mark that,[
R1086 T1468 T1470 amod HOIL-1L,protein
R1087 T1469 T1470 amod interacting,protein
R1088 T1470 T1487 nsubj protein,[
R1089 T1471 T1470 punct (,protein
R109 T187 T185 advmod respectively,responses
R1090 T1472 T1470 appos HOIP,protein
R10900 T13876 T13880 nmod NF-κB,assays
R10901 T13877 T13876 cc and,NF-κB
R10902 T13878 T13876 conj JNK,NF-κB
R10903 T13879 T13880 compound activation,assays
R10904 T13880 T13905 nsubjpass assays,added
R10905 T13881 T13880 prep For,assays
R10906 T13882 T13881 pobj activation,For
R10907 T13883 T13882 prep of,activation
R10908 T13884 T13883 pobj CD40,of
R10909 T13885 T13881 pobj signaling,For
R1091 T1473 T1470 punct ),protein
R10910 T13886 T13885 punct ",",signaling
R10911 T13887 T13888 nummod 5,×
R10912 T13888 T13892 dep ×,cells
R10913 T13889 T13892 nummod 104,cells
R10914 T13890 T13892 nummod HI5,cells
R10915 T13891 T13892 compound insect,cells
R10916 T13892 T13880 appos cells,assays
R10917 T13893 T13892 cc or,cells
R10918 T13894 T13896 nummod HI5,cells
R10919 T13895 T13896 compound insect,cells
R1092 T1474 T1470 punct ",",protein
R10920 T13896 T13892 conj cells,cells
R10921 T13897 T13896 acl expressing,cells
R10922 T13898 T13899 compound mouse,CD154
R10923 T13899 T13897 dobj CD154,expressing
R10924 T13900 T13899 punct (,CD154
R10925 T13901 T13902 compound CD40,ligand
R10926 T13902 T13899 appos ligand,CD154
R10927 T13903 T13899 punct ),CD154
R10928 T13904 T13905 auxpass were,added
R10929 T13905 T13905 ROOT added,added
R1093 T1475 T1477 det a,ligase
R10930 T13906 T13905 prep to,added
R10931 T13907 T13911 nummod 1,cells
R10932 T13908 T13911 nummod ×,cells
R10933 T13909 T13911 nummod 106,cells
R10934 T13910 T13911 compound B,cells
R10935 T13911 T13906 pobj cells,to
R10936 T13912 T13905 punct .,added
R10937 T13913 T13915 nsubjpass Cells,centrifuged
R10938 T13914 T13915 auxpass were,centrifuged
R10939 T13915 T13915 ROOT centrifuged,centrifuged
R1094 T1476 T1477 compound ubiquitin,ligase
R10940 T13916 T13915 prep for,centrifuged
R10941 T13917 T13918 nummod 1,minute
R10942 T13918 T13916 pobj minute,for
R10943 T13919 T13915 prep at,centrifuged
R10944 T13920 T13922 nummod 400,g
R10945 T13921 T13922 punct x,g
R10946 T13922 T13919 pobj g,at
R10947 T13923 T13922 punct (,g
R10948 T13924 T13925 aux to,promote
R10949 T13925 T13922 acl promote,g
R1095 T1477 T1470 appos ligase,protein
R10950 T13926 T13925 dobj contact,promote
R10951 T13927 T13926 prep between,contact
R10952 T13928 T13929 amod ligand,cells
R10953 T13929 T13927 pobj cells,between
R10954 T13930 T13929 cc and,cells
R10955 T13931 T13932 compound B,cells
R10956 T13932 T13929 conj cells,cells
R10957 T13933 T13925 punct ),promote
R10958 T13934 T13935 advmod then,incubated
R10959 T13935 T13922 dep incubated,g
R1096 T1478 T1480 nsubj that,catalyze
R10960 T13936 T13935 prep at,incubated
R10961 T13937 T13938 nummod 37,°
R10962 T13938 T13939 nummod °,C
R10963 T13939 T13936 pobj C,at
R10964 T13940 T13935 prep for,incubated
R10965 T13941 T13940 pobj times,for
R10966 T13942 T13935 conj indicated,incubated
R10967 T13943 T13942 prep in,indicated
R10968 T13944 T13943 pobj Fig.,in
R10969 T13945 T13944 nummod 4,Fig.
R1097 T1479 T1480 aux can,catalyze
R10970 T13946 T13915 punct .,centrifuged
R10971 T13947 T13952 prep Following,chilled
R10972 T13948 T13947 pobj stimulation,Following
R10973 T13949 T13952 punct ",",chilled
R10974 T13950 T13952 nsubjpass cells,chilled
R10975 T13951 T13952 auxpass were,chilled
R10976 T13952 T13952 ROOT chilled,chilled
R10977 T13953 T13952 prep on,chilled
R10978 T13954 T13953 pobj ice,on
R10979 T13955 T13952 prep for,chilled
R1098 T1480 T1477 relcl catalyze,ligase
R10980 T13956 T13957 nummod 2,minutes
R10981 T13957 T13955 pobj minutes,for
R10982 T13958 T13952 punct .,chilled
R10983 T13959 T13960 compound Cell,pellets
R10984 T13960 T13962 nsubjpass pellets,dissolved
R10985 T13961 T13962 auxpass were,dissolved
R10986 T13962 T13962 ROOT dissolved,dissolved
R10987 T13963 T13962 prep in,dissolved
R10988 T13964 T13965 compound 2X,SDS-PAGE
R10989 T13965 T13967 compound SDS-PAGE,buffer
R1099 T1481 T1482 det the,assembly
R10990 T13966 T13967 compound loading,buffer
R10991 T13967 T13963 pobj buffer,in
R10992 T13968 T13962 punct ",",dissolved
R10993 T13969 T13962 advcl sonicated,dissolved
R10994 T13970 T13969 punct ",",sonicated
R10995 T13971 T13969 cc and,sonicated
R10996 T13972 T13969 conj heated,sonicated
R10997 T13973 T13972 prep for,heated
R10998 T13974 T13975 nummod 5,minutes
R10999 T13975 T13973 pobj minutes,for
R110 T188 T176 punct .,provides
R1100 T1482 T1480 dobj assembly,catalyze
R11000 T13976 T13975 prep at,minutes
R11001 T13977 T13978 nummod 95,°
R11002 T13978 T13982 nummod °,lysates
R11003 T13979 T13980 compound C.,Total
R11004 T13980 T13981 compound Total,cell
R11005 T13981 T13982 compound cell,lysates
R11006 T13982 T13976 pobj lysates,at
R11007 T13983 T13982 punct (,lysates
R11008 T13984 T13988 nummod 1,equivalents
R11009 T13985 T13988 nummod ×,equivalents
R1101 T1483 T1482 prep of,assembly
R11010 T13986 T13988 nummod 105,equivalents
R11011 T13987 T13988 compound cell,equivalents
R11012 T13988 T13982 appos equivalents,lysates
R11013 T13989 T13988 prep per,equivalents
R11014 T13990 T13989 pobj lane,per
R11015 T13991 T13962 punct ),dissolved
R11016 T13992 T13993 auxpass were,fractionated
R11017 T13993 T13993 ROOT fractionated,fractionated
R11018 T13994 T13993 agent by,fractionated
R11019 T13995 T13994 pobj SDS-PAGE,by
R1102 T1484 T1486 amod linear,chains
R11020 T13996 T13993 cc and,fractionated
R11021 T13997 T13993 conj blotted,fractionated
R11022 T13998 T13997 prep to,blotted
R11023 T13999 T14000 compound polyvinylidine,fluoride
R11024 T14000 T13998 pobj fluoride,to
R11025 T14001 T14000 punct (,fluoride
R11026 T14002 T14000 appos PVDF,fluoride
R11027 T14003 T14000 punct ),fluoride
R11028 T14004 T13993 nsubjpass membranes,fractionated
R11029 T14005 T13993 punct .,fractionated
R1103 T1485 T1486 compound polyubiquitin,chains
R11030 T14006 T14008 nsubjpass Membranes,probed
R11031 T14007 T14008 auxpass were,probed
R11032 T14008 T14008 ROOT probed,probed
R11033 T14009 T14008 prep with,probed
R11034 T14010 T14009 pobj antibodies,with
R11035 T14011 T14012 mark as,indicated
R11036 T14012 T14008 advcl indicated,probed
R11037 T14013 T14012 prep in,indicated
R11038 T14014 T14013 pobj Fig.,in
R11039 T14015 T14014 nummod 4,Fig.
R1104 T1486 T1483 pobj chains,of
R11040 T14016 T14008 punct .,probed
R11041 T14017 T14018 amod Chemiluminescent,detection
R11042 T14018 T14018 ROOT detection,detection
R11043 T14019 T14024 punct (,used
R11044 T14020 T14021 compound Pierce,Biotechnology
R11045 T14021 T14024 nsubjpass Biotechnology,used
R11046 T14022 T14021 punct ),Biotechnology
R11047 T14023 T14024 auxpass was,used
R11048 T14024 T14024 ROOT used,used
R11049 T14025 T14024 prep for,used
R1105 T1487 T1466 ccomp [,demonstrated
R11050 T14026 T14027 det the,visualization
R11051 T14027 T14025 pobj visualization,for
R11052 T14028 T14027 prep of,visualization
R11053 T14029 T14028 pobj bands,of
R11054 T14030 T14029 prep on,bands
R11055 T14031 T14032 amod Western,blots
R11056 T14032 T14030 pobj blots,on
R11057 T14033 T14024 punct .,used
R11058 T14034 T14038 nsubjpass Images,recorded
R11059 T14035 T14034 prep of,Images
R1106 T1488 T1489 nummod 5,]
R11060 T14036 T14035 pobj blots,of
R11061 T14037 T14038 auxpass were,recorded
R11062 T14038 T14038 ROOT recorded,recorded
R11063 T14039 T14038 prep with,recorded
R11064 T14040 T14043 det a,system
R11065 T14041 T14043 amod low-light,system
R11066 T14042 T14043 compound imaging,system
R11067 T14043 T14039 pobj system,with
R11068 T14044 T14043 punct (,system
R11069 T14045 T14043 appos LAS4000,system
R1107 T1489 T1487 dobj ],[
R11070 T14046 T14043 punct ",",system
R11071 T14047 T14049 compound Fuji,Systems
R11072 T14048 T14049 compound Medical,Systems
R11073 T14049 T14043 appos Systems,system
R11074 T14050 T14043 punct ),system
R11075 T14051 T14039 cc and,with
R11076 T14052 T14039 conj on,with
R11077 T14053 T14054 compound X-ray,film
R11078 T14054 T14052 pobj film,on
R11079 T14055 T14038 punct .,recorded
R1108 T1490 T1492 punct ",",recruited
R1109 T1491 T1492 auxpass is,recruited
R111 T189 T195 prep In,has
R1110 T1492 T1466 conj recruited,demonstrated
R1111 T1493 T1492 prep to,recruited
R1112 T1494 T1493 pobj CD40,to
R1113 T1495 T1492 prep in,recruited
R1114 T1496 T1498 det a,manner
R1115 T1497 T1498 amod TRAF2-dependent,manner
R1116 T1498 T1495 pobj manner,in
R1117 T1499 T1492 prep following,recruited
R1118 T1500 T1499 pobj engagement,following
R1119 T1501 T1500 prep of,engagement
R112 T190 T191 compound B,cells
R1120 T1502 T1501 pobj CD40,of
R1121 T1503 T1500 prep by,engagement
R1122 T1504 T1506 amod agonistic,[
R1123 T1505 T1506 compound antibody,[
R1124 T1506 T1508 nmod [,]
R1125 T1507 T1508 nummod 6,]
R11257 T14284 T14285 compound Immunoprecipitations,Immunoprecipitation
R11258 T14285 T14289 nsubjpass Immunoprecipitation,performed
R11259 T14286 T14285 prep of,Immunoprecipitation
R1126 T1508 T1503 pobj ],by
R11260 T14287 T14286 pobj CD40,of
R11261 T14288 T14289 auxpass was,performed
R11262 T14289 T14289 ROOT performed,performed
R11263 T14290 T14289 agent by,performed
R11264 T14291 T14293 amod activated,capture
R11265 T14292 T14293 compound receptor,capture
R11266 T14293 T14290 pobj capture,by
R11267 T14294 T14289 punct ",",performed
R11268 T14295 T14297 mark as,described
R11269 T14296 T14297 advmod previously,described
R1127 T1509 T1466 punct .,demonstrated
R11270 T14297 T14289 advcl described,performed
R11271 T14298 T14300 nmod [,]
R11272 T14299 T14300 nummod 6,]
R11273 T14300 T14297 dobj ],described
R11274 T14301 T14289 punct .,performed
R11275 T14302 T14316 advmod Briefly,incubated
R11276 T14303 T14316 punct ",",incubated
R11277 T14304 T14305 nummod 3,×
R11278 T14305 T14316 nsubjpass ×,incubated
R11279 T14306 T14307 nummod 107,cells
R1128 T1510 T1513 det These,findings
R11280 T14307 T14305 appos cells,×
R11281 T14308 T14307 punct (,cells
R11282 T14309 T14311 nummod A20,J
R11283 T14310 T14311 nummod .2,J
R11284 T14311 T14307 appos J,cells
R11285 T14312 T14311 cc and,J
R11286 T14313 T14311 conj derivatives,J
R11287 T14314 T14307 punct ),cells
R11288 T14315 T14316 auxpass were,incubated
R11289 T14316 T14316 ROOT incubated,incubated
R1129 T1511 T1510 cc and,These
R11290 T14317 T14316 prep for,incubated
R11291 T14318 T14319 nummod 60,minutes
R11292 T14319 T14317 pobj minutes,for
R11293 T14320 T14319 prep at,minutes
R11294 T14321 T14322 compound room,temperature
R11295 T14322 T14320 pobj temperature,at
R11296 T14323 T14316 prep with,incubated
R11297 T14324 T14325 nummod 10,µl
R11298 T14325 T14329 compound µl,beads
R11299 T14326 T14327 amod magnetic,protein
R113 T191 T189 pobj cells,In
R1130 T1512 T1513 amod other,findings
R11300 T14327 T14329 compound protein,beads
R11301 T14328 T14329 compound G,beads
R11302 T14329 T14323 pobj beads,with
R11303 T14330 T14329 punct (,beads
R11304 T14331 T14329 appos Dynal,beads
R11305 T14332 T14329 punct ),beads
R11306 T14333 T14316 advcl pre-coated,incubated
R11307 T14334 T14333 prep with,pre-coated
R11308 T14335 T14336 nummod 10,µg
R11309 T14336 T14339 compound µg,IgG
R1131 T1513 T1514 nsubj findings,led
R11310 T14337 T14339 compound goat,IgG
R11311 T14338 T14339 compound anti-rat,IgG
R11312 T14339 T14334 pobj IgG,with
R11313 T14340 T14341 punct (,Jackson
R11314 T14341 T14339 appos Jackson,IgG
R11315 T14342 T14341 punct ),Jackson
R11316 T14343 T14339 punct ",",IgG
R11317 T14344 T14339 cc and,IgG
R11318 T14345 T14347 nummod 10,anti-CD40
R11319 T14346 T14347 compound µg,anti-CD40
R1132 T1514 T1514 ROOT led,led
R11320 T14347 T14339 conj anti-CD40,IgG
R11321 T14348 T14349 punct (,1C10
R11322 T14349 T14347 appos 1C10,anti-CD40
R11323 T14350 T14349 punct ),1C10
R11324 T14351 T14347 cc or,anti-CD40
R11325 T14352 T14355 det an,antibody
R11326 T14353 T14355 nmod isotype,antibody
R11327 T14354 T14355 compound control,antibody
R11328 T14355 T14347 conj antibody,anti-CD40
R11329 T14356 T14355 punct (,antibody
R1133 T1515 T1514 dobj us,led
R11330 T14357 T14355 appos mAb72,antibody
R11331 T14358 T14355 punct ),antibody
R11332 T14359 T14316 punct .,incubated
R11333 T14360 T14363 nsubjpass Cells/beads,pelleted
R11334 T14361 T14363 auxpass were,pelleted
R11335 T14362 T14363 advmod then,pelleted
R11336 T14363 T14363 ROOT pelleted,pelleted
R11337 T14364 T14363 agent by,pelleted
R11338 T14365 T14364 pobj centrifugation,by
R11339 T14366 T14363 cc and,pelleted
R1134 T1516 T1517 aux to,hypothesize
R11340 T14367 T14363 conj lysed,pelleted
R11341 T14368 T14367 prep in,lysed
R11342 T14369 T14368 pobj buffer,in
R11343 T14370 T14369 acl containing,buffer
R11344 T14371 T14372 nummod 1,%
R11345 T14372 T14374 compound %,X100
R11346 T14373 T14374 compound Triton,X100
R11347 T14374 T14370 dobj X100,containing
R11348 T14375 T14363 punct .,pelleted
R11349 T14376 T14378 nsubjpass Beads,washed
R1135 T1517 T1514 xcomp hypothesize,led
R11350 T14377 T14378 auxpass were,washed
R11351 T14378 T14378 ROOT washed,washed
R11352 T14379 T14378 prep with,washed
R11353 T14380 T14381 compound lysis,buffer
R11354 T14381 T14379 pobj buffer,with
R11355 T14382 T14383 aux to,remove
R11356 T14383 T14378 advcl remove,washed
R11357 T14384 T14385 amod unbound,material
R11358 T14385 T14383 dobj material,remove
R11359 T14386 T14378 punct .,washed
R1136 T1518 T1520 det that,functions
R11360 T14387 T14397 prep In,dephosphorylated
R11361 T14388 T14389 det some,experiments
R11362 T14389 T14387 pobj experiments,In
R11363 T14390 T14397 punct ",",dephosphorylated
R11364 T14391 T14396 nsubj material,was
R11365 T14392 T14391 acl associated,material
R11366 T14393 T14392 prep with,associated
R11367 T14394 T14395 det the,beads
R11368 T14395 T14393 pobj beads,with
R11369 T14396 T14397 auxpass was,dephosphorylated
R1137 T1519 T1520 compound HOIP,functions
R11370 T14397 T14397 ROOT dephosphorylated,dephosphorylated
R11371 T14398 T14397 prep with,dephosphorylated
R11372 T14399 T14400 compound lambda,phosphatase
R11373 T14400 T14398 pobj phosphatase,with
R11374 T14401 T14404 punct (,BioLabs
R11375 T14402 T14404 compound New,BioLabs
R11376 T14403 T14404 compound England,BioLabs
R11377 T14404 T14400 appos BioLabs,phosphatase
R11378 T14405 T14404 punct ),BioLabs
R11379 T14406 T14397 prep as,dephosphorylated
R1138 T1520 T1517 dobj functions,hypothesize
R11380 T14407 T14406 prep per,as
R11381 T14408 T14410 poss manufacturer,instructions
R11382 T14409 T14408 case 's,manufacturer
R11383 T14410 T14407 pobj instructions,per
R11384 T14411 T14397 punct .,dephosphorylated
R11385 T14412 T14414 nsubjpass Beads,resuspended
R11386 T14413 T14414 auxpass were,resuspended
R11387 T14414 T14414 ROOT resuspended,resuspended
R11388 T14415 T14414 prep in,resuspended
R11389 T14416 T14417 compound 2X,SDS-PAGE
R1139 T1521 T1520 dobj downstream,functions
R11390 T14417 T14419 compound SDS-PAGE,buffer
R11391 T14418 T14419 compound sample,buffer
R11392 T14419 T14415 pobj buffer,in
R11393 T14420 T14414 cc and,resuspended
R11394 T14421 T14414 conj heated,resuspended
R11395 T14422 T14421 prep for,heated
R11396 T14423 T14424 nummod 5,minutes
R11397 T14424 T14422 pobj minutes,for
R11398 T14425 T14424 prep at,minutes
R11399 T14426 T14430 nummod 95,eluted
R114 T192 T195 punct ",",has
R1140 T1522 T1521 prep of,downstream
R11400 T14427 T14430 nummod °,eluted
R11401 T14428 T14429 compound C.,Material
R11402 T14429 T14430 compound Material,eluted
R11403 T14430 T14414 conj eluted,resuspended
R11404 T14431 T14430 prep from,eluted
R11405 T14432 T14433 det the,beads
R11406 T14433 T14431 pobj beads,from
R11407 T14434 T14435 auxpass was,fractionated
R11408 T14435 T14435 ROOT fractionated,fractionated
R11409 T14436 T14435 agent by,fractionated
R1141 T1523 T1522 pobj TRAF2,of
R11410 T14437 T14436 pobj SDS-PAGE,by
R11411 T14438 T14435 cc and,fractionated
R11412 T14439 T14435 conj transferred,fractionated
R11413 T14440 T14439 prep to,transferred
R11414 T14441 T14442 compound PVDF,membranes
R11415 T14442 T14440 pobj membranes,to
R11416 T14443 T14442 prep for,membranes
R11417 T14444 T14445 amod Western,blotting
R11418 T14445 T14443 pobj blotting,for
R11419 T14446 T14435 punct .,fractionated
R1142 T1524 T1521 prep in,downstream
R11420 T14447 T14453 prep In,cultured
R11421 T14448 T14449 det some,experiments
R11422 T14449 T14447 pobj experiments,In
R11423 T14450 T14453 punct ",",cultured
R11424 T14451 T14453 nsubjpass cells,cultured
R11425 T14452 T14453 auxpass were,cultured
R11426 T14453 T14453 ROOT cultured,cultured
R11427 T14454 T14453 prep for,cultured
R11428 T14455 T14456 nummod 6,hrs
R11429 T14456 T14454 pobj hrs,for
R1143 T1525 T1526 det the,CD40
R11430 T14457 T14456 prep with,hrs
R11431 T14458 T14459 nummod 25,µM
R11432 T14459 T14462 nmod µM,peptide
R11433 T14460 T14462 amod antennapedia-linked,peptide
R11434 T14461 T14462 compound SMAC-N7,peptide
R11435 T14462 T14457 pobj peptide,with
R11436 T14463 T14462 punct (,peptide
R11437 T14464 T14462 appos Calbiochem,peptide
R11438 T14465 T14462 punct ),peptide
R11439 T14466 T14462 cc or,peptide
R1144 T1526 T1524 pobj CD40,in
R11440 T14467 T14469 det an,volume
R11441 T14468 T14469 amod appropriate,volume
R11442 T14469 T14453 dobj volume,cultured
R11443 T14470 T14469 prep of,volume
R11444 T14471 T14472 det the,solvent
R11445 T14472 T14470 pobj solvent,of
R11446 T14473 T14469 acl used,volume
R11447 T14474 T14473 prep for,used
R11448 T14475 T14476 det the,peptide
R11449 T14476 T14474 pobj peptide,for
R1145 T1527 T1520 prep signaling,functions
R11450 T14477 T14476 punct (,peptide
R11451 T14478 T14476 appos DMSO,peptide
R11452 T14479 T14476 punct ),peptide
R11453 T14480 T14453 punct .,cultured
R11454 T14481 T14494 prep After,stimulated
R11455 T14482 T14481 pobj incubation,After
R11456 T14483 T14494 punct ",",stimulated
R11457 T14484 T14494 nsubjpass cells,stimulated
R11458 T14485 T14484 punct (,cells
R11459 T14486 T14484 prep in,cells
R1146 T1528 T1527 dobj pathway,signaling
R11460 T14487 T14486 pobj peptide,in
R11461 T14488 T14486 punct -,in
R11462 T14489 T14486 cc or,in
R11463 T14490 T14491 amod DMSO-containing,medium
R11464 T14491 T14484 conj medium,cells
R11465 T14492 T14484 punct ),cells
R11466 T14493 T14494 auxpass were,stimulated
R11467 T14494 T14494 ROOT stimulated,stimulated
R11468 T14495 T14494 prep with,stimulated
R11469 T14496 T14497 amod antibody-coated,beads
R1147 T1529 T1520 cc and,functions
R11470 T14497 T14495 pobj beads,with
R11471 T14498 T14499 mark as,outlined
R11472 T14499 T14494 advcl outlined,stimulated
R11473 T14500 T14499 prep above,outlined
R11474 T14501 T14494 punct .,stimulated
R1148 T1530 T1532 mark that,is
R1149 T1531 T1532 nsubj HOIP,is
R115 T193 T194 nsubj CD40,signaling
R1150 T1532 T1520 conj is,functions
R1151 T1533 T1532 acomp necessary,is
R1152 T1534 T1532 prep for,is
R1153 T1535 T1536 det the,activation
R1154 T1536 T1534 pobj activation,for
R1155 T1537 T1536 prep of,activation
R1156 T1538 T1537 pobj NF-κB,of
R1157 T1539 T1536 cc and,activation
R1158 T1540 T1543 advmod possibly,molecules
R1159 T1541 T1543 amod other,molecules
R116 T194 T195 nsubj signaling,has
R1160 T1542 T1543 amod signaling,molecules
R1161 T1543 T1536 conj molecules,activation
R1162 T1544 T1514 punct .,led
R1163 T1545 T1546 aux To,test
R1164 T1546 T1551 advcl test,employed
R1165 T1547 T1548 det this,hypothesis
R1166 T1548 T1546 dobj hypothesis,test
R1167 T1549 T1551 punct ",",employed
R1168 T1550 T1551 nsubj we,employed
R1169 T1551 T1551 ROOT employed,employed
R117 T195 T195 ROOT has,has
R1170 T1552 T1554 amod somatic,gene
R11703 T14807 T14808 compound HOIP,gene
R11704 T14808 T14809 compound gene,targeting
R11705 T14809 T14809 ROOT targeting,targeting
R11706 T14810 T14809 punct .,targeting
R11707 T14811 T14833 punct (,shown
R11708 T14812 T14833 advmod A,shown
R11709 T14813 T14814 punct ),Regions
R1171 T1553 T1554 compound cell,gene
R11710 T14814 T14833 nsubjpass Regions,shown
R11711 T14815 T14814 prep of,Regions
R11712 T14816 T14818 amod Rnf31,sequence
R11713 T14817 T14818 compound gene,sequence
R11714 T14818 T14815 pobj sequence,of
R11715 T14819 T14818 punct (,sequence
R11716 T14820 T14818 appos bottom,sequence
R11717 T14821 T14818 punct ),sequence
R11718 T14822 T14814 acl used,Regions
R11719 T14823 T14822 prep in,used
R1172 T1554 T1551 dobj gene,employed
R11720 T14824 T14826 det the,gene
R11721 T14825 T14826 compound HOIP,gene
R11722 T14826 T14823 pobj gene,in
R11723 T14827 T14814 acl targeting,Regions
R11724 T14828 T14827 dobj vector,targeting
R11725 T14829 T14828 punct (,vector
R11726 T14830 T14828 amod top,vector
R11727 T14831 T14814 punct ),Regions
R11728 T14832 T14833 auxpass are,shown
R11729 T14833 T14833 ROOT shown,shown
R1173 T1555 T1554 acl targeting,gene
R11730 T14834 T14833 punct .,shown
R11731 T14835 T14841 nsubj Arrows,indicate
R11732 T14836 T14837 punct (,F
R11733 T14837 T14835 appos F,Arrows
R11734 T14838 T14837 cc and,F
R11735 T14839 T14837 conj R,F
R11736 T14840 T14835 punct ),Arrows
R11737 T14841 T14841 ROOT indicate,indicate
R11738 T14842 T14843 amod approximate,positions
R11739 T14843 T14841 dobj positions,indicate
R1174 T1556 T1557 aux to,ablate
R11740 T14844 T14843 prep of,positions
R11741 T14845 T14844 pobj sequences,of
R11742 T14846 T14841 advmod homologous,indicate
R11743 T14847 T14846 prep to,homologous
R11744 T14848 T14847 pobj oligonucleotides,to
R11745 T14849 T14848 acl used,oligonucleotides
R11746 T14850 T14849 prep for,used
R11747 T14851 T14852 amod PCR-mediated,detection
R11748 T14852 T14850 pobj detection,for
R11749 T14853 T14852 prep of,detection
R1175 T1557 T1555 xcomp ablate,targeting
R11750 T14854 T14855 amod homologous,recombination
R11751 T14855 T14853 pobj recombination,of
R11752 T14856 T14841 punct .,indicate
R11753 T14857 T14858 amod Homologous,recombination
R11754 T14858 T14864 nsubj recombination,resulted
R11755 T14859 T14858 prep of,recombination
R11756 T14860 T14861 det the,vector
R11757 T14861 T14859 pobj vector,of
R11758 T14862 T14858 prep with,recombination
R11759 T14863 T14862 pobj Rnf31,with
R1176 T1558 T1557 dobj expression,ablate
R11760 T14864 T14864 ROOT resulted,resulted
R11761 T14865 T14864 prep in,resulted
R11762 T14866 T14869 det a,deletion
R11763 T14867 T14869 amod small,deletion
R11764 T14868 T14869 amod chromosomal,deletion
R11765 T14869 T14865 pobj deletion,in
R11766 T14870 T14869 cc and,deletion
R11767 T14871 T14873 det the,insertion
R11768 T14872 T14873 amod in-frame,insertion
R11769 T14873 T14869 conj insertion,deletion
R1177 T1559 T1558 prep of,expression
R11770 T14874 T14873 prep of,insertion
R11771 T14875 T14876 compound neomycin,phosphotransferase
R11772 T14876 T14874 pobj phosphotransferase,of
R11773 T14877 T14878 punct (,NeoR
R11774 T14878 T14876 appos NeoR,phosphotransferase
R11775 T14879 T14864 punct ),resulted
R11776 T14880 T14864 prep into,resulted
R11777 T14881 T14885 det the,sequence
R11778 T14882 T14885 amod amino-terminal,sequence
R11779 T14883 T14885 compound HOIP,sequence
R1178 T1560 T1559 pobj HOIP,of
R11780 T14884 T14885 compound coding,sequence
R11781 T14885 T14880 pobj sequence,into
R11782 T14886 T14864 punct .,resulted
R11783 T14887 T14889 det A,toxin
R11784 T14888 T14889 compound diphtheria,toxin
R11785 T14889 T14897 nsubj toxin,facilitates
R11786 T14890 T14889 punct (,toxin
R11787 T14891 T14889 appos DT,toxin
R11788 T14892 T14889 punct ),toxin
R11789 T14893 T14889 appos cassette,toxin
R1179 T1561 T1557 prep in,ablate
R11790 T14894 T14893 prep in,cassette
R11791 T14895 T14896 det the,vector
R11792 T14896 T14894 pobj vector,in
R11793 T14897 T14897 ROOT facilitates,facilitates
R11794 T14898 T14900 det the,selection
R11795 T14899 T14900 amod negative,selection
R11796 T14900 T14897 dobj selection,facilitates
R11797 T14901 T14900 prep of,selection
R11798 T14902 T14901 pobj cells,of
R11799 T14903 T14911 prep in,takes
R118 T196 T198 det a,role
R1180 T1562 T1566 det a,line
R11800 T14904 T14903 pobj which,in
R11801 T14905 T14907 amod random,insertion
R11802 T14906 T14907 amod chromosomal,insertion
R11803 T14907 T14911 nsubj insertion,takes
R11804 T14908 T14907 prep of,insertion
R11805 T14909 T14910 det the,vector
R11806 T14910 T14908 pobj vector,of
R11807 T14911 T14900 relcl takes,selection
R11808 T14912 T14911 dobj place,takes
R11809 T14913 T14897 punct .,facilitates
R1181 T1563 T1565 compound mouse,cell
R11810 T14914 T14915 compound LoxP,sequences
R11811 T14915 T14916 nsubj sequences,allow
R11812 T14916 T14916 ROOT allow,allow
R11813 T14917 T14919 det the,deletion
R11814 T14918 T14919 amod Cre-mediated,deletion
R11815 T14919 T14916 dobj deletion,allow
R11816 T14920 T14919 prep of,deletion
R11817 T14921 T14924 det the,sequence
R11818 T14922 T14924 compound NeoR,sequence
R11819 T14923 T14924 compound coding,sequence
R1182 T1564 T1565 compound B,cell
R11820 T14924 T14920 pobj sequence,of
R11821 T14925 T14916 punct .,allow
R11822 T14926 T14928 det The,sequence
R11823 T14927 T14928 compound SV40pA,sequence
R11824 T14928 T14929 nsubj sequence,helps
R11825 T14929 T14929 ROOT helps,helps
R11826 T14930 T14931 aux to,ensure
R11827 T14931 T14929 xcomp ensure,helps
R11828 T14932 T14931 dobj disruption,ensure
R11829 T14933 T14932 prep of,disruption
R1183 T1565 T1566 compound cell,line
R11830 T14934 T14935 compound gene,expression
R11831 T14935 T14933 pobj expression,of
R11832 T14936 T14931 prep after,ensure
R11833 T14937 T14936 pobj deletion,after
R11834 T14938 T14937 prep of,deletion
R11835 T14939 T14941 det the,sequence
R11836 T14940 T14941 compound NeoR,sequence
R11837 T14941 T14938 pobj sequence,of
R11838 T14942 T14929 punct .,helps
R11839 T14943 T14946 punct (,Anti-HOIP
R1184 T1566 T1561 pobj line,in
R11840 T14944 T14946 nmod B,Anti-HOIP
R11841 T14945 T14946 punct ),Anti-HOIP
R11842 T14946 T14950 nmod Anti-HOIP,blots
R11843 T14947 T14946 cc and,Anti-HOIP
R11844 T14948 T14949 compound anti-FLAG,Western
R11845 T14949 T14946 conj Western,Anti-HOIP
R11846 T14950 T14950 ROOT blots,blots
R11847 T14951 T14950 prep of,blots
R11848 T14952 T14953 compound cell,lysates
R11849 T14953 T14951 pobj lysates,of
R1185 T1567 T1569 nsubj that,proven
R11850 T14954 T14950 prep from,blots
R11851 T14955 T14958 compound A20,cells
R11852 T14956 T14957 compound .2,J
R11853 T14957 T14958 compound J,cells
R11854 T14958 T14954 pobj cells,from
R11855 T14959 T14958 cc and,cells
R11856 T14960 T14958 conj HOIP-deficient,cells
R11857 T14961 T14967 punct (,clones
R11858 T14962 T14967 dep HOIP,clones
R11859 T14963 T14967 punct -,clones
R1186 T1568 T1569 aux has,proven
R11860 T14964 T14967 amod /,clones
R11861 T14965 T14964 punct -,/
R11862 T14966 T14964 punct ),/
R11863 T14967 T14958 conj clones,cells
R11864 T14968 T14950 punct .,blots
R11865 T14969 T14971 det A,decrease
R11866 T14970 T14971 amod partial,decrease
R11867 T14971 T14976 nsubj decrease,is
R11868 T14972 T14971 prep in,decrease
R11869 T14973 T14975 compound HOIP,expression
R1187 T1569 T1566 relcl proven,line
R11870 T14974 T14975 compound protein,expression
R11871 T14975 T14972 pobj expression,in
R11872 T14976 T14976 ROOT is,is
R11873 T14977 T14976 acomp evident,is
R11874 T14978 T14976 prep in,is
R11875 T14979 T14978 pobj cells,in
R11876 T14980 T14979 acl following,cells
R11877 T14981 T14980 dobj disruption,following
R11878 T14982 T14981 prep of,disruption
R11879 T14983 T14984 nummod one,copy
R1188 T1570 T1571 aux to,be
R11880 T14984 T14982 pobj copy,of
R11881 T14985 T14984 prep of,copy
R11882 T14986 T14985 pobj Rnf31,of
R11883 T14987 T14986 punct (,Rnf31
R11884 T14988 T14990 neg HOIP,/
R11885 T14989 T14990 compound −,/
R11886 T14990 T14986 parataxis /,Rnf31
R11887 T14991 T14986 appos +,Rnf31
R11888 T14992 T14986 punct ),Rnf31
R11889 T14993 T14976 punct .,is
R1189 T1571 T1569 xcomp be,proven
R11890 T14994 T14995 compound HOIP,expression
R11891 T14995 T15016 nsubjpass expression,shown
R11892 T14996 T14995 prep in,expression
R11893 T14997 T14996 pobj clones,in
R11894 T14998 T14997 acl reconstituted,clones
R11895 T14999 T14998 prep with,reconstituted
R11896 T15000 T15003 det an,vector
R11897 T15001 T15003 amod empty,vector
R11898 T15002 T15003 compound retroviral,vector
R11899 T15003 T14999 pobj vector,with
R119 T197 T198 amod major,role
R1190 T1572 T1574 det a,model
R11900 T15004 T15003 punct (,vector
R11901 T15005 T15003 appos pMIP,vector
R11902 T15006 T15003 punct ),vector
R11903 T15007 T15003 cc or,vector
R11904 T15008 T15010 det a,vector
R11905 T15009 T15010 amod retroviral,vector
R11906 T15010 T15003 conj vector,vector
R11907 T15011 T15010 acl encoding,vector
R11908 T15012 T15013 compound FLAG-tagged,HOIP
R11909 T15013 T15011 dobj HOIP,encoding
R1191 T1573 T1574 amod useful,model
R11910 T15014 T15016 auxpass is,shown
R11911 T15015 T15016 advmod also,shown
R11912 T15016 T15016 ROOT shown,shown
R11913 T15017 T15016 punct .,shown
R11914 T15018 T15020 amod Approximate,weight
R11915 T15019 T15020 amod molecular,weight
R11916 T15020 T15023 nsubj weight,is
R11917 T15021 T15020 prep of,weight
R11918 T15022 T15021 pobj HOIP,of
R11919 T15023 T15023 ROOT is,is
R1192 T1574 T1571 attr model,be
R11920 T15024 T15025 nummod 120,kD
R11921 T15025 T15023 attr kD,is
R11922 T15026 T15023 punct .,is
R1193 T1575 T1574 prep for,model
R1194 T1576 T1577 compound B,cell
R1195 T1577 T1575 pobj cell,for
R1196 T1578 T1577 nummod CD40,cell
R1197 T1579 T1574 acl signaling,model
R1198 T1580 T1579 dobj [,signaling
R1199 T1581 T1580 nummod 7,[
R120 T198 T195 dobj role,has
R1200 T1582 T1580 conj ],[
R1201 T1583 T1582 punct ",",]
R1202 T1584 T1586 nmod [,]
R1203 T1585 T1586 nummod 8,]
R1204 T1586 T1582 conj ],]
R1205 T1587 T1586 punct ",",]
R1206 T1588 T1590 det [,]
R1207 T1589 T1590 nummod 9,]
R1208 T1590 T1586 appos ],]
R1209 T1591 T1551 punct .,employed
R121 T199 T198 prep in,role
R1210 T1592 T1593 nsubj We,found
R1211 T1593 T1593 ROOT found,found
R1212 T1594 T1608 mark that,was
R1213 T1595 T1597 det the,upregulation
R1214 T1596 T1597 amod CD40-induced,upregulation
R1215 T1597 T1608 nsubj upregulation,was
R1216 T1598 T1597 prep of,upregulation
R1217 T1599 T1598 pobj CD80,of
R1218 T1600 T1599 punct (,CD80
R12180 T15392 T15394 amod CD40-mediated,upregulation
R12181 T15393 T15394 compound CD80,upregulation
R12182 T15394 T15395 nsubj upregulation,is
R12183 T15395 T15395 ROOT is,is
R12184 T15396 T15395 acomp defective,is
R12185 T15397 T15396 prep in,defective
R12186 T15398 T15399 amod HOIP-deficient,cells
R12187 T15399 T15397 pobj cells,in
R12188 T15400 T15395 punct .,is
R12189 T15401 T15403 nsubjpass Cells,incubated
R1219 T1601 T1603 det a,molecule
R12190 T15402 T15403 auxpass were,incubated
R12191 T15403 T15403 ROOT incubated,incubated
R12192 T15404 T15403 prep for,incubated
R12193 T15405 T15406 nummod 72,hrs
R12194 T15406 T15404 pobj hrs,for
R12195 T15407 T15403 prep with,incubated
R12196 T15408 T15411 det an,antibody
R12197 T15409 T15410 amod isotype,control
R12198 T15410 T15411 compound control,antibody
R12199 T15411 T15407 pobj antibody,with
R122 T200 T199 pcomp regulating,in
R1220 T1602 T1603 amod costimulatory,molecule
R12200 T15412 T15413 punct (,left
R12201 T15413 T15403 advcl left,incubated
R12202 T15414 T15413 punct ),left
R12203 T15415 T15413 cc or,left
R12204 T15416 T15417 amod anti-CD40,antibody
R12205 T15417 T15403 conj antibody,incubated
R12206 T15418 T15419 punct (,right
R12207 T15419 T15417 parataxis right,antibody
R12208 T15420 T15419 punct ),right
R12209 T15421 T15403 cc and,incubated
R1221 T1603 T1599 appos molecule,CD80
R12210 T15422 T15423 advmod then,stained
R12211 T15423 T15403 conj stained,incubated
R12212 T15424 T15423 prep for,stained
R12213 T15425 T15424 pobj expression,for
R12214 T15426 T15425 prep of,expression
R12215 T15427 T15426 pobj CD80,of
R12216 T15428 T15429 punct (,filled
R12217 T15429 T15430 amod filled,profiles
R12218 T15430 T15431 nsubj profiles,indicate
R12219 T15431 T15423 ccomp indicate,stained
R1222 T1604 T1603 prep for,molecule
R12220 T15432 T15431 ccomp staining,indicate
R12221 T15433 T15432 prep with,staining
R12222 T15434 T15435 amod anti-CD80,antibody
R12223 T15435 T15433 pobj antibody,with
R12224 T15436 T15431 punct ;,indicate
R12225 T15437 T15438 amod open,profiles
R12226 T15438 T15440 nsubj profiles,staining
R12227 T15439 T15440 aux are,staining
R12228 T15440 T15403 conj staining,incubated
R12229 T15441 T15440 prep with,staining
R1223 T1605 T1606 compound T,cells
R12230 T15442 T15445 det an,antibody
R12231 T15443 T15444 amod isotype,control
R12232 T15444 T15445 compound control,antibody
R12233 T15445 T15441 pobj antibody,with
R12234 T15446 T15440 punct ),staining
R12235 T15447 T15440 punct .,staining
R12236 T15448 T15464 nsubj Transduction,reversed
R12237 T15449 T15448 prep of,Transduction
R12238 T15450 T15451 det both,HOIP-deficient
R12239 T15451 T15449 pobj HOIP-deficient,of
R1224 T1606 T1604 pobj cells,for
R12240 T15452 T15451 punct (,HOIP-deficient
R12241 T15453 T15455 amod HOIP,/
R12242 T15454 T15455 punct -,/
R12243 T15455 T15455 ROOT /,/
R12244 T15456 T15455 punct -,/
R12245 T15457 T15455 punct ),/
R12246 T15458 T15464 nsubj clones,reversed
R12247 T15459 T15458 prep with,clones
R12248 T15460 T15461 det a,retrovirus
R12249 T15461 T15459 pobj retrovirus,with
R1225 T1607 T1599 punct ),CD80
R12250 T15462 T15461 acl encoding,retrovirus
R12251 T15463 T15464 nsubj FLAG-HOIP,reversed
R12252 T15464 T15464 ROOT reversed,reversed
R12253 T15465 T15467 amod defective,upregulation
R12254 T15466 T15467 compound CD80,upregulation
R12255 T15467 T15464 dobj upregulation,reversed
R12256 T15468 T15464 punct ",",reversed
R12257 T15469 T15470 mark while,transduction
R12258 T15470 T15464 advcl transduction,reversed
R12259 T15471 T15470 prep with,transduction
R1226 T1608 T1593 ccomp was,found
R12260 T15472 T15474 det an,vector
R12261 T15473 T15474 amod empty,vector
R12262 T15474 T15471 pobj vector,with
R12263 T15475 T15474 punct (,vector
R12264 T15476 T15474 appos pMIP,vector
R12265 T15477 T15474 punct ),vector
R12266 T15478 T15480 aux did,.
R12267 T15479 T15478 neg not,did
R12268 T15480 T15464 punct .,reversed
R12269 T15481 T15482 amod Similar,results
R1227 T1609 T1608 acomp defective,was
R12270 T15482 T15484 nsubjpass results,obtained
R12271 T15483 T15484 auxpass were,obtained
R12272 T15484 T15484 ROOT obtained,obtained
R12273 T15485 T15484 prep in,obtained
R12274 T15486 T15488 det a,experiment
R12275 T15487 T15488 amod second,experiment
R12276 T15488 T15485 pobj experiment,in
R12277 T15489 T15484 punct .,obtained
R1228 T1610 T1609 prep in,defective
R1229 T1611 T1612 amod HOIP-deficient,cells
R123 T201 T202 compound cell,proliferation
R1230 T1612 T1610 pobj cells,in
R1231 T1613 T1593 punct .,found
R1232 T1614 T1620 advmod Similarly,driven
R1233 T1615 T1620 punct ",",driven
R1234 T1616 T1617 det the,CD40
R1235 T1617 T1620 nsubj CD40,driven
R1236 T1618 T1617 cc and,CD40
R1237 T1619 T1617 conj IL-4,CD40
R1238 T1620 T1620 ROOT driven,driven
R1239 T1621 T1620 dobj production,driven
R124 T202 T200 dobj proliferation,regulating
R1240 T1622 T1621 prep of,production
R1241 T1623 T1624 compound germline,transcripts
R1242 T1624 T1622 pobj transcripts,of
R12429 T15742 T15743 compound GLε,transcription
R1243 T1625 T1624 prep from,transcripts
R12430 T15743 T15744 nsubj transcription,is
R12431 T15744 T15744 ROOT is,is
R12432 T15745 T15744 acomp defective,is
R12433 T15746 T15745 prep in,defective
R12434 T15747 T15749 amod CD40-stimulated,cells
R12435 T15748 T15749 amod HOIP-deficient,cells
R12436 T15749 T15746 pobj cells,in
R12437 T15750 T15744 punct .,is
R12438 T15751 T15753 nummod A20,J
R12439 T15752 T15753 nummod .2,J
R1244 T1626 T1631 det the,locus
R12440 T15753 T15756 nmod J,cells
R12441 T15754 T15753 cc and,J
R12442 T15755 T15753 conj HOIP-deficient,J
R12443 T15756 T15756 ROOT cells,cells
R12444 T15757 T15756 acl transduced,cells
R12445 T15758 T15757 prep with,transduced
R12446 T15759 T15762 det an,vector
R12447 T15760 T15762 amod empty,vector
R12448 T15761 T15762 compound retroviral,vector
R12449 T15762 T15758 pobj vector,with
R1245 T1627 T1631 amod immunoglobulin,locus
R12450 T15763 T15762 punct (,vector
R12451 T15764 T15768 nummod A20,pMIP
R12452 T15765 T15768 nummod .2,pMIP
R12453 T15766 T15768 nmod J,pMIP
R12454 T15767 T15768 nummod +,pMIP
R12455 T15768 T15762 appos pMIP,vector
R12456 T15769 T15768 cc and,pMIP
R12457 T15770 T15768 conj HOIP,pMIP
R12458 T15771 T15762 punct -,vector
R12459 T15772 T15762 punct /,vector
R1246 T1628 T1631 amod epsilon,locus
R12460 T15773 T15762 punct -,vector
R12461 T15774 T15775 compound +,pMIP
R12462 T15775 T15762 appos pMIP,vector
R12463 T15776 T15775 punct ",",pMIP
R12464 T15777 T15775 advmod respectively,pMIP
R12465 T15778 T15775 punct ),pMIP
R12466 T15779 T15775 punct ",",pMIP
R12467 T15780 T15775 cc or,pMIP
R12468 T15781 T15782 amod HOIP-deficient,cells
R12469 T15782 T15775 conj cells,pMIP
R1247 T1629 T1631 amod heavy,locus
R12470 T15783 T15762 acl transduced,vector
R12471 T15784 T15783 prep with,transduced
R12472 T15785 T15787 det a,retrovirus
R12473 T15786 T15787 amod HOIP-encoding,retrovirus
R12474 T15787 T15784 pobj retrovirus,with
R12475 T15788 T15797 punct (,cultured
R12476 T15789 T15793 punct HOIP,+
R12477 T15790 T15793 punct -,+
R12478 T15791 T15793 punct /,+
R12479 T15792 T15793 punct -,+
R1248 T1630 T1631 compound chain,locus
R12480 T15793 T15797 nsubjpass +,cultured
R12481 T15794 T15793 npadvmod HOIP,+
R12482 T15795 T15797 punct ),cultured
R12483 T15796 T15797 auxpass were,cultured
R12484 T15797 T15787 parataxis cultured,retrovirus
R12485 T15798 T15797 npadvmod overnight,cultured
R12486 T15799 T15797 prep with,cultured
R12487 T15800 T15801 amod agonistic,anti-CD40
R12488 T15801 T15799 pobj anti-CD40,with
R12489 T15802 T15801 cc or,anti-CD40
R1249 T1631 T1625 pobj locus,from
R12490 T15803 T15806 det an,antibody
R12491 T15804 T15806 nmod isotype,antibody
R12492 T15805 T15806 compound control,antibody
R12493 T15806 T15801 conj antibody,anti-CD40
R12494 T15807 T15806 punct (,antibody
R12495 T15808 T15806 parataxis iso,antibody
R12496 T15809 T15808 punct ),iso
R12497 T15810 T15806 punct ",",antibody
R12498 T15811 T15806 conj with,antibody
R12499 T15812 T15811 cc or,with
R125 T203 T205 punct ",",production
R1250 T1632 T1620 punct ",",driven
R12500 T15813 T15811 conj without,with
R12501 T15814 T15813 pobj IL-4,without
R12502 T15815 T15756 punct .,cells
R12503 T15816 T15818 nsubjpass RNA,isolated
R12504 T15817 T15818 auxpass was,isolated
R12505 T15818 T15818 ROOT isolated,isolated
R12506 T15819 T15818 prep from,isolated
R12507 T15820 T15821 det the,cells
R12508 T15821 T15819 pobj cells,from
R12509 T15822 T15818 punct ",",isolated
R1251 T1633 T1634 det an,event
R12510 T15823 T15818 conj reverse-transcribed,isolated
R12511 T15824 T15823 punct ",",reverse-transcribed
R12512 T15825 T15823 cc and,reverse-transcribed
R12513 T15826 T15827 advmod then,subjected
R12514 T15827 T15823 conj subjected,reverse-transcribed
R12515 T15828 T15827 prep to,subjected
R12516 T15829 T15830 amod quantitative,PCR
R12517 T15830 T15828 pobj PCR,to
R12518 T15831 T15832 aux to,determine
R12519 T15832 T15827 advcl determine,subjected
R1252 T1634 T1620 npadvmod event,driven
R12520 T15833 T15832 dobj levels,determine
R12521 T15834 T15833 prep of,levels
R12522 T15835 T15836 compound GLε,transcripts
R12523 T15836 T15834 pobj transcripts,of
R12524 T15837 T15818 punct .,isolated
R12525 T15838 T15839 nsubj Results,were
R12526 T15839 T15839 ROOT were,were
R12527 T15840 T15839 acomp normalized,were
R12528 T15841 T15840 prep to,normalized
R12529 T15842 T15843 det the,levels
R1253 T1635 T1636 nsubj that,precedes
R12530 T15843 T15841 pobj levels,to
R12531 T15844 T15843 prep of,levels
R12532 T15845 T15846 amod Hprt1,transcripts
R12533 T15846 T15844 pobj transcripts,of
R12534 T15847 T15843 prep in,levels
R12535 T15848 T15849 det each,sample
R12536 T15849 T15847 pobj sample,in
R12537 T15850 T15839 punct .,were
R12538 T15851 T15852 nsubj Symbols,indicate
R12539 T15852 T15852 ROOT indicate,indicate
R1254 T1636 T1634 relcl precedes,event
R12540 T15853 T15854 det the,values
R12541 T15854 T15852 dobj values,indicate
R12542 T15855 T15852 prep from,indicate
R12543 T15856 T15857 compound duplicate,cultures
R12544 T15857 T15855 pobj cultures,from
R12545 T15858 T15857 punct (,cultures
R12546 T15859 T15860 det a,line
R12547 T15860 T15861 nsubj line,indicates
R12548 T15861 T15852 ccomp indicates,indicate
R12549 T15862 T15863 det the,mean
R1255 T1637 T1639 compound immunoglobulin,rearrangement
R12550 T15863 T15861 dobj mean,indicates
R12551 T15864 T15863 prep of,mean
R12552 T15865 T15867 det the,values
R12553 T15866 T15867 nummod two,values
R12554 T15867 T15864 pobj values,of
R12555 T15868 T15861 punct ),indicates
R12556 T15869 T15852 punct .,indicate
R12557 T15870 T15871 amod Similar,results
R12558 T15871 T15873 nsubjpass results,obtained
R12559 T15872 T15873 auxpass were,obtained
R1256 T1638 T1639 compound gene,rearrangement
R12560 T15873 T15873 ROOT obtained,obtained
R12561 T15874 T15873 prep in,obtained
R12562 T15875 T15877 det a,experiment
R12563 T15876 T15877 amod second,experiment
R12564 T15877 T15874 pobj experiment,in
R12565 T15878 T15874 cc and,in
R12566 T15879 T15874 conj in,in
R12567 T15880 T15882 det an,experiment
R12568 T15881 T15882 amod additional,experiment
R12569 T15882 T15879 pobj experiment,in
R1257 T1639 T1636 dobj rearrangement,precedes
R12570 T15883 T15882 prep with,experiment
R12571 T15884 T15887 det a,clone
R12572 T15885 T15887 amod second,clone
R12573 T15886 T15887 amod HOIP-deficient,clone
R12574 T15887 T15883 pobj clone,with
R12575 T15888 T15873 punct .,obtained
R1258 T1640 T1639 cc and,rearrangement
R1259 T1641 T1642 compound isotype,switching
R126 T204 T205 compound antibody,production
R1260 T1642 T1639 conj switching,rearrangement
R1261 T1643 T1620 punct ",",driven
R1262 T1644 T1620 conj was,driven
R1263 T1645 T1644 acomp defective,was
R1264 T1646 T1644 prep in,was
R1265 T1647 T1648 det the,absence
R1266 T1648 T1646 pobj absence,in
R1267 T1649 T1648 prep of,absence
R1268 T1650 T1649 pobj HOIP,of
R1269 T1651 T1620 punct .,driven
R127 T205 T198 conj production,role
R1270 T1652 T1654 nsubj We,found
R1271 T1653 T1654 advmod also,found
R1272 T1654 T1654 ROOT found,found
R1273 T1655 T1671 mark that,was
R1274 T1656 T1658 det the,activation
R1275 T1657 T1658 amod CD40-mediated,activation
R12757 T16192 T16193 amod CD40-induced,activation
R12758 T16193 T16198 nsubj activation,is
R12759 T16194 T16193 prep of,activation
R1276 T1658 T1671 nsubj activation,was
R12760 T16195 T16194 pobj NF-κB,of
R12761 T16196 T16195 cc and,NF-κB
R12762 T16197 T16195 conj JNK,NF-κB
R12763 T16198 T16198 ROOT is,is
R12764 T16199 T16198 acomp defective,is
R12765 T16200 T16199 prep in,defective
R12766 T16201 T16202 amod HOIP-deficient,cells
R12767 T16202 T16200 pobj cells,in
R12768 T16203 T16198 punct .,is
R12769 T16204 T16205 amod CD40-mediated,signaling
R1277 T1659 T1658 prep of,activation
R12770 T16205 T16206 nsubj signaling,was
R12771 T16206 T16206 ROOT was,was
R12772 T16207 T16206 acomp defective,was
R12773 T16208 T16207 prep in,defective
R12774 T16209 T16210 amod HOIP-deficient,cells
R12775 T16210 T16208 pobj cells,in
R12776 T16211 T16216 punct (,+
R12777 T16212 T16216 punct HOIP,+
R12778 T16213 T16216 punct -,+
R12779 T16214 T16216 punct /,+
R1278 T1660 T1659 pobj NF-κB,of
R12780 T16215 T16216 punct -,+
R12781 T16216 T16206 parataxis +,was
R12782 T16217 T16216 npadvmod pMIP,+
R12783 T16218 T16216 punct ),+
R12784 T16219 T16216 punct ",",+
R12785 T16220 T16216 cc but,+
R12786 T16221 T16216 conj intact,+
R12787 T16222 T16221 prep in,intact
R12788 T16223 T16224 amod parental,cells
R12789 T16224 T16222 pobj cells,in
R1279 T1661 T1660 cc and,NF-κB
R12790 T16225 T16216 conj transduced,+
R12791 T16226 T16225 prep with,transduced
R12792 T16227 T16230 det an,vector
R12793 T16228 T16230 amod empty,vector
R12794 T16229 T16230 compound retroviral,vector
R12795 T16230 T16226 pobj vector,with
R12796 T16231 T16230 punct (,vector
R12797 T16232 T16236 nummod A20,pMIP
R12798 T16233 T16236 nummod .2,pMIP
R12799 T16234 T16236 nmod J,pMIP
R128 T206 T205 punct ",",production
R1280 T1662 T1667 det the,kinase
R12800 T16235 T16236 nummod +,pMIP
R12801 T16236 T16230 appos pMIP,vector
R12802 T16237 T16236 punct ),pMIP
R12803 T16238 T16226 cc and,with
R12804 T16239 T16225 prep in,transduced
R12805 T16240 T16241 amod HOIP-deficient,cells
R12806 T16241 T16239 pobj cells,in
R12807 T16242 T16225 conj transduced,transduced
R12808 T16243 T16242 prep with,transduced
R12809 T16244 T16246 det a,vector
R1281 T1663 T1667 amod stress-activated,kinase
R12810 T16245 T16246 amod retroviral,vector
R12811 T16246 T16243 pobj vector,with
R12812 T16247 T16248 compound encoding,HOIP
R12813 T16248 T16246 appos HOIP,vector
R12814 T16249 T16255 punct (,HOIP
R12815 T16250 T16255 compound HOIP,HOIP
R12816 T16251 T16254 punct -,+
R12817 T16252 T16254 punct /,+
R12818 T16253 T16254 punct -,+
R12819 T16254 T16255 compound +,HOIP
R1282 T1664 T1667 nmod protein,kinase
R12820 T16255 T16248 appos HOIP,HOIP
R12821 T16256 T16255 punct ),HOIP
R12822 T16257 T16206 punct .,was
R12823 T16258 T16260 nsubjpass Cells,activated
R12824 T16259 T16260 auxpass were,activated
R12825 T16260 T16260 ROOT activated,activated
R12826 T16261 T16260 prep with,activated
R12827 T16262 T16264 amod CD154-expressing,cells
R12828 T16263 T16264 compound insect,cells
R12829 T16264 T16261 pobj cells,with
R1283 T1665 T1667 nmod kinase,kinase
R12830 T16265 T16260 prep for,activated
R12831 T16266 T16267 det the,times
R12832 T16267 T16265 pobj times,for
R12833 T16268 T16260 conj indicated,activated
R12834 T16269 T16260 punct .,activated
R12835 T16270 T16277 prep As,stimulated
R12836 T16271 T16273 det a,control
R12837 T16272 T16273 amod negative,control
R12838 T16273 T16270 pobj control,As
R12839 T16274 T16277 punct ",",stimulated
R1284 T1666 T1667 amod c-Jun,kinase
R12840 T16275 T16277 nsubjpass cells,stimulated
R12841 T16276 T16277 auxpass were,stimulated
R12842 T16277 T16277 ROOT stimulated,stimulated
R12843 T16278 T16277 prep with,stimulated
R12844 T16279 T16280 amod insect,cells
R12845 T16280 T16278 pobj cells,with
R12846 T16281 T16280 acl lacking,cells
R12847 T16282 T16281 dobj CD154,lacking
R12848 T16283 T16282 punct (,CD154
R12849 T16284 T16287 nummod 5,point
R1285 T1667 T1660 conj kinase,NF-κB
R12850 T16285 T16286 amod minute,time
R12851 T16286 T16287 compound time,point
R12852 T16287 T16282 appos point,CD154
R12853 T16288 T16289 advmod only,)
R12854 T16289 T16282 punct ),CD154
R12855 T16290 T16277 punct .,stimulated
R12856 T16291 T16292 amod Western,blots
R12857 T16292 T16297 nsubjpass blots,probed
R12858 T16293 T16292 prep of,blots
R12859 T16294 T16295 amod whole-cell,lysates
R1286 T1668 T1669 punct (,JNK
R12860 T16295 T16293 pobj lysates,of
R12861 T16296 T16297 auxpass were,probed
R12862 T16297 T16297 ROOT probed,probed
R12863 T16298 T16297 prep with,probed
R12864 T16299 T16301 det the,antibodies
R12865 T16300 T16301 amod indicated,antibodies
R12866 T16301 T16298 pobj antibodies,with
R12867 T16302 T16297 punct .,probed
R12868 T16303 T16312 nmod Phospho-IκBα,blots
R12869 T16304 T16305 punct (,pIκBα
R1287 T1669 T1667 appos JNK,kinase
R12870 T16305 T16303 appos pIκBα,Phospho-IκBα
R12871 T16306 T16305 punct ),pIκBα
R12872 T16307 T16303 cc and,Phospho-IκBα
R12873 T16308 T16303 conj phospho-JNK,Phospho-IκBα
R12874 T16309 T16308 punct (,phospho-JNK
R12875 T16310 T16308 appos pJNK,phospho-JNK
R12876 T16311 T16308 punct ),phospho-JNK
R12877 T16312 T16314 nsubjpass blots,stripped
R12878 T16313 T16314 auxpass were,stripped
R12879 T16314 T16314 ROOT stripped,stripped
R1288 T1670 T1667 punct ),kinase
R12880 T16315 T16314 cc and,stripped
R12881 T16316 T16314 conj reprobed,stripped
R12882 T16317 T16316 prep for,reprobed
R12883 T16318 T16319 amod total,IκBα
R12884 T16319 T16317 pobj IκBα,for
R12885 T16320 T16316 cc and,reprobed
R12886 T16321 T16322 compound total,JNK
R12887 T16322 T16316 conj JNK,reprobed
R12888 T16323 T16322 punct ",",JNK
R12889 T16324 T16322 conj respectively,JNK
R1289 T1671 T1654 ccomp was,found
R12890 T16325 T16328 punct (,recognize
R12891 T16326 T16327 amod anti-JNK,antibodies
R12892 T16327 T16328 nsubj antibodies,recognize
R12893 T16328 T16322 parataxis recognize,JNK
R12894 T16329 T16328 dobj p46,recognize
R12895 T16330 T16329 cc and,p46
R12896 T16331 T16329 conj p54,p46
R12897 T16332 T16322 conj isoforms,JNK
R12898 T16333 T16322 punct ),JNK
R12899 T16334 T16314 punct .,stripped
R129 T207 T205 cc and,production
R1290 T1672 T1671 acomp defective,was
R12900 T16335 T16336 compound IκBα,blots
R12901 T16336 T16339 nsubjpass blots,reprobed
R12902 T16337 T16339 auxpass were,reprobed
R12903 T16338 T16337 advmod also,were
R12904 T16339 T16339 ROOT reprobed,reprobed
R12905 T16340 T16339 prep for,reprobed
R12906 T16341 T16343 nsubj actin,demonstrate
R12907 T16342 T16343 aux to,demonstrate
R12908 T16343 T16339 advcl demonstrate,reprobed
R12909 T16344 T16345 amod equal,lane
R1291 T1673 T1672 prep in,defective
R12910 T16345 T16346 compound lane,loading
R12911 T16346 T16343 dobj loading,demonstrate
R12912 T16347 T16339 punct .,reprobed
R12913 T16348 T16349 amod Molecular,weights
R12914 T16349 T16351 nsubjpass weights,indicated
R12915 T16350 T16351 auxpass are,indicated
R12916 T16351 T16351 ROOT indicated,indicated
R12917 T16352 T16351 prep at,indicated
R12918 T16353 T16352 pobj right,at
R12919 T16354 T16351 punct .,indicated
R1292 T1674 T1675 amod HOIP-deficient,cells
R12920 T16355 T16356 amod Similar,results
R12921 T16356 T16358 nsubjpass results,obtained
R12922 T16357 T16358 auxpass were,obtained
R12923 T16358 T16358 ROOT obtained,obtained
R12924 T16359 T16358 prep in,obtained
R12925 T16360 T16362 det a,experiment
R12926 T16361 T16362 amod second,experiment
R12927 T16362 T16359 pobj experiment,in
R12928 T16363 T16359 cc and,in
R12929 T16364 T16358 prep in,obtained
R1293 T1675 T1673 pobj cells,in
R12930 T16365 T16367 nummod two,experiments
R12931 T16366 T16367 amod additional,experiments
R12932 T16367 T16364 pobj experiments,in
R12933 T16368 T16367 prep with,experiments
R12934 T16369 T16372 det a,clone
R12935 T16370 T16372 amod second,clone
R12936 T16371 T16372 amod HOIP-deficient,clone
R12937 T16372 T16368 pobj clone,with
R12938 T16373 T16358 punct .,obtained
R1294 T1676 T1654 punct .,found
R1295 T1677 T1694 nsubj Consistent,was
R1296 T1678 T1677 prep with,Consistent
R1297 T1679 T1681 amod impaired,activation
R1298 T1680 T1681 compound NF-κB,activation
R1299 T1681 T1678 pobj activation,with
R130 T208 T209 compound memory,B
R1300 T1682 T1681 punct ",",activation
R1301 T1683 T1681 conj association,activation
R1302 T1684 T1681 prep of,activation
R1303 T1685 T1687 det the,kinase
R1304 T1686 T1687 compound IκB,kinase
R1305 T1687 T1684 pobj kinase,of
R1306 T1688 T1689 punct (,IKK
R1307 T1689 T1687 appos IKK,kinase
R1308 T1690 T1689 punct ),IKK
R1309 T1691 T1681 amod complex,activation
R131 T209 T211 compound B,development
R1310 T1692 T1681 prep with,activation
R1311 T1693 T1692 pobj CD40,with
R1312 T1694 T1694 ROOT was,was
R1313 T1695 T1694 acomp undetectable,was
R1314 T1696 T1695 prep in,undetectable
R1315 T1697 T1698 amod HOIP-deficient,cells
R1316 T1698 T1696 pobj cells,in
R1317 T1699 T1694 punct .,was
R1318 T1700 T1704 advmod Together,indicate
R13183 T16830 T16831 compound IKK,recruitment
R13184 T16831 T16831 ROOT recruitment,recruitment
R13185 T16832 T16831 prep to,recruitment
R13186 T16833 T16834 det the,CD40
R13187 T16834 T16832 pobj CD40,to
R13188 T16835 T16831 acl signaling,recruitment
R13189 T16836 T16837 nsubj complex,is
R1319 T1701 T1704 punct ",",indicate
R13190 T16837 T16835 ccomp is,signaling
R13191 T16838 T16837 acomp defective,is
R13192 T16839 T16838 prep in,defective
R13193 T16840 T16841 amod HOIP-deficient,cells
R13194 T16841 T16839 pobj cells,in
R13195 T16842 T16831 punct .,recruitment
R13196 T16843 T16849 punct (,reduces
R13197 T16844 T16848 det A,treatment
R13198 T16845 T16846 punct ),SMAC
R13199 T16846 T16848 nmod SMAC,treatment
R132 T210 T211 compound cell,development
R1320 T1702 T1703 poss our,results
R13200 T16847 T16848 compound peptide,treatment
R13201 T16848 T16849 nsubj treatment,reduces
R13202 T16849 T16849 ROOT reduces,reduces
R13203 T16850 T16849 dobj recruitment,reduces
R13204 T16851 T16850 prep of,recruitment
R13205 T16852 T16851 pobj cIAP1,of
R13206 T16853 T16849 prep to,reduces
R13207 T16854 T16853 pobj CD40,to
R13208 T16855 T16849 cc and,reduces
R13209 T16856 T16857 aux may,modify
R1321 T1703 T1704 nsubj results,indicate
R13210 T16857 T16849 conj modify,reduces
R13211 T16858 T16859 compound HOIP,recruitment
R13212 T16859 T16857 dobj recruitment,modify
R13213 T16860 T16849 punct .,reduces
R13214 T16861 T16864 nummod A20,cells
R13215 T16862 T16864 nummod .2,cells
R13216 T16863 T16864 compound J,cells
R13217 T16864 T16866 nsubjpass cells,incubated
R13218 T16865 T16866 auxpass were,incubated
R13219 T16866 T16866 ROOT incubated,incubated
R1322 T1704 T1704 ROOT indicate,indicate
R13220 T16867 T16866 prep for,incubated
R13221 T16868 T16869 nummod six,hours
R13222 T16869 T16867 pobj hours,for
R13223 T16870 T16866 prep with,incubated
R13224 T16871 T16873 amod membrane-permeable,peptide
R13225 T16872 T16873 compound SMAC-N7,peptide
R13226 T16873 T16870 pobj peptide,with
R13227 T16874 T16873 cc or,peptide
R13228 T16875 T16876 nummod 1.5,%
R13229 T16876 T16877 compound %,DMSO
R1323 T1705 T1707 mark that,plays
R13230 T16877 T16873 conj DMSO,peptide
R13231 T16878 T16880 punct (,used
R13232 T16879 T16880 advmod solvent,used
R13233 T16880 T16866 advcl used,incubated
R13234 T16881 T16880 prep for,used
R13235 T16882 T16883 det the,peptide
R13236 T16883 T16881 pobj peptide,for
R13237 T16884 T16880 punct ),used
R13238 T16885 T16866 punct .,incubated
R13239 T16886 T16893 prep Following,prepared
R1324 T1706 T1707 nsubj HOIP,plays
R13240 T16887 T16888 det the,incubation
R13241 T16888 T16886 pobj incubation,Following
R13242 T16889 T16893 punct ",",prepared
R13243 T16890 T16891 compound cell,lysates
R13244 T16891 T16893 nsubjpass lysates,prepared
R13245 T16892 T16893 auxpass were,prepared
R13246 T16893 T16893 ROOT prepared,prepared
R13247 T16894 T16893 punct ",",prepared
R13248 T16895 T16893 conj fractionated,prepared
R13249 T16896 T16895 agent by,fractionated
R1325 T1707 T1704 ccomp plays,indicate
R13250 T16897 T16896 pobj SDS-PAGE,by
R13251 T16898 T16895 punct ",",fractionated
R13252 T16899 T16895 cc and,fractionated
R13253 T16900 T16895 conj evaluated,fractionated
R13254 T16901 T16900 agent by,evaluated
R13255 T16902 T16903 amod Western,blot
R13256 T16903 T16901 pobj blot,by
R13257 T16904 T16905 punct (,lanes
R13258 T16905 T16900 parataxis lanes,evaluated
R13259 T16906 T16905 nummod 1,lanes
R1326 T1708 T1710 det a,role
R13260 T16907 T16905 cc and,lanes
R13261 T16908 T16905 conj 2,lanes
R13262 T16909 T16905 punct ),lanes
R13263 T16910 T16893 punct .,prepared
R13264 T16911 T16912 nsubj Cells,incubated
R13265 T16912 T16912 ROOT incubated,incubated
R13266 T16913 T16912 prep with,incubated
R13267 T16914 T16913 pobj DMSO,with
R13268 T16915 T16914 cc or,DMSO
R13269 T16916 T16914 conj SMAC-N7,DMSO
R1327 T1709 T1710 amod critical,role
R13270 T16917 T16919 auxpass were,stimulated
R13271 T16918 T16919 advmod also,stimulated
R13272 T16919 T16912 conj stimulated,incubated
R13273 T16920 T16919 prep with,stimulated
R13274 T16921 T16922 amod magnetic,beads
R13275 T16922 T16920 pobj beads,with
R13276 T16923 T16922 acl coated,beads
R13277 T16924 T16923 prep with,coated
R13278 T16925 T16924 pobj anti-CD40,with
R13279 T16926 T17018 prep with,transduced
R1328 T1710 T1707 dobj role,plays
R13280 T16927 T16925 cc or,anti-CD40
R13281 T16928 T16931 det an,antibody
R13282 T16929 T16930 compound isotype,control
R13283 T16930 T16931 compound control,antibody
R13284 T16931 T16925 conj antibody,anti-CD40
R13285 T16932 T16919 punct .,stimulated
R13286 T16933 T16937 amod Immunoprecipitated,material
R13287 T16934 T16937 punct (,material
R13288 T16935 T16937 amod IP,material
R13289 T16936 T16937 punct ),material
R1329 T1711 T1710 prep in,role
R13290 T16937 T16943 nsubjpass material,loaded
R13291 T16938 T16937 acl bound,material
R13292 T16939 T16938 prep to,bound
R13293 T16940 T16941 det the,beads
R13294 T16941 T16939 pobj beads,to
R13295 T16942 T16943 auxpass was,loaded
R13296 T16943 T16943 ROOT loaded,loaded
R13297 T16944 T16943 prep in,loaded
R13298 T16945 T16944 pobj lanes,in
R13299 T16946 T16943 advmod 3-5,loaded
R133 T211 T205 conj development,production
R1330 T1712 T1713 det the,activation
R13300 T16947 T16943 punct .,loaded
R13301 T16948 T16952 nsubj Samples,lysates
R13302 T16949 T16948 prep of,Samples
R13303 T16950 T16951 det the,cell
R13304 T16951 T16949 pobj cell,of
R13305 T16952 T16952 ROOT lysates,lysates
R13306 T16953 T16955 mark after,appear
R13307 T16954 T16955 nsubj immunoprecipitation,appear
R13308 T16955 T16952 advcl appear,lysates
R13309 T16956 T16955 prep in,appear
R1331 T1713 T1711 pobj activation,in
R13310 T16957 T16956 pobj lanes,in
R13311 T16958 T16955 npadvmod 6,appear
R13312 T16959 T16958 appos 8,6
R13313 T16960 T16952 punct .,lysates
R13314 T16961 T16962 amod Western,blots
R13315 T16962 T16964 nsubjpass blots,probed
R13316 T16963 T16964 auxpass were,probed
R13317 T16964 T16964 ROOT probed,probed
R13318 T16965 T16964 prep with,probed
R13319 T16966 T16965 pobj antibodies,with
R1332 T1714 T1713 prep of,activation
R13320 T16967 T16966 amod specific,antibodies
R13321 T16968 T16967 prep for,specific
R13322 T16969 T16968 pobj cIAP1,for
R13323 T16970 T16969 punct ",",cIAP1
R13324 T16971 T16969 appos TRAF2,cIAP1
R13325 T16972 T16969 punct ",",cIAP1
R13326 T16973 T16969 appos TRAF3,cIAP1
R13327 T16974 T16964 punct ",",probed
R13328 T16975 T16964 cc and,probed
R13329 T16976 T16981 dep HOIP,indicated
R1333 T1715 T1714 pcomp signaling,of
R13330 T16977 T16981 punct (,indicated
R13331 T16978 T16980 amod approximate,weights
R13332 T16979 T16980 amod molecular,weights
R13333 T16980 T16981 nsubj weights,indicated
R13334 T16981 T16964 conj indicated,probed
R13335 T16982 T16981 prep on,indicated
R13336 T16983 T16982 pobj left,on
R13337 T16984 T16981 punct ),indicated
R13338 T16985 T16981 punct .,indicated
R13339 T16986 T16987 amod Similar,results
R1334 T1716 T1715 dobj pathways,signaling
R13340 T16987 T16989 nsubjpass results,obtained
R13341 T16988 T16989 auxpass were,obtained
R13342 T16989 T16989 ROOT obtained,obtained
R13343 T16990 T16989 prep in,obtained
R13344 T16991 T16993 nummod two,experiments
R13345 T16992 T16993 amod additional,experiments
R13346 T16993 T16990 pobj experiments,in
R13347 T16994 T16989 punct .,obtained
R13348 T16995 T16998 punct (,CD40
R13349 T16996 T16998 nmod B,CD40
R1335 T1717 T1718 nsubj that,regulate
R13350 T16997 T16998 punct ),CD40
R13351 T16998 T17000 nsubjpass CD40,isolated
R13352 T16999 T17000 auxpass was,isolated
R13353 T17000 T17000 ROOT isolated,isolated
R13354 T17001 T17000 agent by,isolated
R13355 T17002 T17001 pobj immunoprecipitation,by
R13356 T17003 T17004 punct (,as
R13357 T17004 T17000 prep as,isolated
R13358 T17005 T17004 prep in,as
R13359 T17006 T17007 punct (,A
R1336 T1718 T1716 relcl regulate,pathways
R13360 T17007 T17005 pobj A,in
R13361 T17008 T17007 punct ),A
R13362 T17009 T17004 punct ),as
R13363 T17010 T17000 prep from,isolated
R13364 T17011 T17014 nummod A20,cells
R13365 T17012 T17014 nummod .2,cells
R13366 T17013 T17014 compound J,cells
R13367 T17014 T17010 pobj cells,from
R13368 T17015 T17014 cc and,cells
R13369 T17016 T17017 amod HOIP-deficient,cells
R1337 T1719 T1720 amod cellular,responses
R13370 T17017 T17014 conj cells,cells
R13371 T17018 T17000 conj transduced,isolated
R13372 T17019 T17022 det an,vector
R13373 T17020 T17022 amod empty,vector
R13374 T17021 T17022 compound retroviral,vector
R13375 T17022 T16926 pobj vector,with
R13376 T17023 T17022 punct (,vector
R13377 T17024 T17022 appos pMIP,vector
R13378 T17025 T17022 punct ),vector
R13379 T17026 T17022 cc or,vector
R1338 T1720 T1718 dobj responses,regulate
R13380 T17027 T17029 det a,vector
R13381 T17028 T17029 amod retroviral,vector
R13382 T17029 T17022 conj vector,vector
R13383 T17030 T17018 advcl encoding,transduced
R13384 T17031 T17030 dobj HOIP,encoding
R13385 T17032 T17000 punct .,isolated
R13386 T17033 T17034 compound Material,immunoprecipitated
R13387 T17034 T17047 nsubjpass immunoprecipitated,examined
R13388 T17035 T17034 prep with,immunoprecipitated
R13389 T17036 T17045 det an,antibody
R1339 T1721 T1718 prep to,regulate
R13390 T17037 T17038 amod isotype,control
R13391 T17038 T17045 nmod control,antibody
R13392 T17039 T17045 nmod antibody,antibody
R13393 T17040 T17041 punct (,isotype
R13394 T17041 T17045 nmod isotype,antibody
R13395 T17042 T17041 punct ),isotype
R13396 T17043 T17041 cc or,isotype
R13397 T17044 T17045 amod anti-CD40,antibody
R13398 T17045 T17035 pobj antibody,with
R13399 T17046 T17047 auxpass was,examined
R134 T212 T195 punct .,has
R1340 T1722 T1723 nummod CD40,engagement
R13400 T17047 T17047 ROOT examined,examined
R13401 T17048 T17047 agent by,examined
R13402 T17049 T17050 amod Western,blotting
R13403 T17050 T17048 pobj blotting,by
R13404 T17051 T17050 prep for,blotting
R13405 T17052 T17051 pobj CD40,for
R13406 T17053 T17052 punct ",",CD40
R13407 T17054 T17052 appos TRAF2,CD40
R13408 T17055 T17052 punct ",",CD40
R13409 T17056 T17052 appos TRAF3,CD40
R1341 T1723 T1721 pobj engagement,to
R13410 T17057 T17052 punct ",",CD40
R13411 T17058 T17052 conj cIAP1,CD40
R13412 T17059 T17058 punct ",",cIAP1
R13413 T17060 T17058 conj HOIP,cIAP1
R13414 T17061 T17060 punct ",",HOIP
R13415 T17062 T17064 compound IKKα,β
R13416 T17063 T17064 compound /,β
R13417 T17064 T17060 conj β,HOIP
R13418 T17065 T17064 punct ",",β
R13419 T17066 T17064 cc and,β
R1342 T1724 T1704 punct .,indicate
R13420 T17067 T17064 conj IKKγ,β
R13421 T17068 T17070 punct (,panels
R13422 T17069 T17070 amod right,panels
R13423 T17070 T17067 appos panels,IKKγ
R13424 T17071 T17067 punct ),IKKγ
R13425 T17072 T17047 punct .,examined
R13426 T17073 T17074 compound HOIP,expression
R13427 T17074 T17076 nsubjpass expression,required
R13428 T17075 T17076 auxpass was,required
R13429 T17076 T17076 ROOT required,required
R13430 T17077 T17076 prep for,required
R13431 T17078 T17077 pobj coprecipitation,for
R13432 T17079 T17078 prep of,coprecipitation
R13433 T17080 T17081 compound IKK,proteins
R13434 T17081 T17079 pobj proteins,of
R13435 T17082 T17076 prep with,required
R13436 T17083 T17082 pobj CD40,with
R13437 T17084 T17076 punct .,required
R13438 T17085 T17086 nsubj Cell,lysates
R13439 T17086 T17086 ROOT lysates,lysates
R13440 T17087 T17086 prep from,lysates
R13441 T17088 T17089 amod unstimulated,cells
R13442 T17089 T17087 pobj cells,from
R13443 T17090 T17091 auxpass are,shown
R13444 T17091 T17086 conj shown,lysates
R13445 T17092 T17091 prep in,shown
R13446 T17093 T17095 det the,panels
R13447 T17094 T17095 amod left,panels
R13448 T17095 T17092 pobj panels,in
R13449 T17096 T17086 punct .,lysates
R13450 T17097 T17098 amod Similar,results
R13451 T17098 T17100 nsubjpass results,obtained
R13452 T17099 T17100 auxpass were,obtained
R13453 T17100 T17100 ROOT obtained,obtained
R13454 T17101 T17100 prep in,obtained
R13455 T17102 T17104 det a,experiment
R13456 T17103 T17104 amod second,experiment
R13457 T17104 T17101 pobj experiment,in
R13458 T17105 T17101 cc and,in
R13459 T17106 T17101 conj in,in
R13460 T17107 T17108 nummod two,experiments
R13461 T17108 T17106 pobj experiments,in
R13462 T17109 T17108 prep with,experiments
R13463 T17110 T17113 det a,clone
R13464 T17111 T17113 amod second,clone
R13465 T17112 T17113 amod HOIP-deficient,clone
R13466 T17113 T17109 pobj clone,with
R13467 T17114 T17100 punct .,obtained
R13468 T17115 T17116 punct (,C
R13469 T17116 T17116 ROOT C,C
R13470 T17117 T17116 punct ),C
R13471 T17118 T17200 pobj blotting,by
R13472 T17119 T17121 aux To,evaluate
R13473 T17120 T17121 advmod further,evaluate
R13474 T17121 T17138 advcl evaluate,/
R13475 T17122 T17123 amod HOIP-dependent,recruitment
R13476 T17123 T17121 dobj recruitment,evaluate
R13477 T17124 T17123 prep of,recruitment
R13478 T17125 T17124 pobj IKKγ,of
R13479 T17126 T17121 prep to,evaluate
R13480 T17127 T17126 pobj CD40,to
R13481 T17128 T17127 punct ",",CD40
R13482 T17129 T17138 ccomp A20,/
R13483 T17130 T17132 nummod .2,cells
R13484 T17131 T17132 compound J,cells
R13485 T17132 T17129 dobj cells,A20
R13486 T17133 T17132 cc or,cells
R13487 T17134 T17132 conj HOIP-deficient,cells
R13488 T17135 T17134 punct (,HOIP-deficient
R13489 T17136 T17138 punct HOIP,/
R13490 T17137 T17138 punct -,/
R13491 T17138 T17138 ROOT /,/
R13492 T17139 T17138 punct -,/
R13493 T17140 T17138 punct ),/
R13494 T17141 T17144 nummod A20,cells
R13495 T17142 T17144 nummod .2,cells
R13496 T17143 T17144 compound J,cells
R13497 T17144 T17146 nsubjpass cells,transduced
R13498 T17145 T17146 auxpass were,transduced
R13499 T17146 T17146 ROOT transduced,transduced
R135 T213 T215 nummod CD40,results
R13500 T17147 T17146 prep with,transduced
R13501 T17148 T17151 det an,vector
R13502 T17149 T17151 amod empty,vector
R13503 T17150 T17151 compound retroviral,vector
R13504 T17151 T17147 pobj vector,with
R13505 T17152 T17151 cc or,vector
R13506 T17153 T17155 det a,construct
R13507 T17154 T17155 amod retroviral,construct
R13508 T17155 T17146 advcl construct,transduced
R13509 T17156 T17155 xcomp encoding,construct
R13510 T17157 T17159 compound BP,IKKγ
R13511 T17158 T17159 compound epitope-tagged,IKKγ
R13512 T17159 T17156 dobj IKKγ,encoding
R13513 T17160 T17161 punct (,noted
R13514 T17161 T17156 advcl noted,encoding
R13515 T17162 T17161 prep in,noted
R13516 T17163 T17164 det the,figure
R13517 T17164 T17162 pobj figure,in
R13518 T17165 T17164 prep as,figure
R13519 T17166 T17165 pobj pMIP,as
R13520 T17167 T17166 cc and,pMIP
R13521 T17168 T17166 conj IKKγ,pMIP
R13522 T17169 T17168 punct ",",IKKγ
R13523 T17170 T17168 conj respectively,IKKγ
R13524 T17171 T17161 punct ),noted
R13525 T17172 T17146 punct .,transduced
R13526 T17173 T17173 ROOT Lysates,Lysates
R13527 T17174 T17175 punct (,lanes
R13528 T17175 T17175 ROOT lanes,lanes
R13529 T17176 T17175 nummod 1,lanes
R13530 T17177 T17184 nmod 3,samples
R13531 T17178 T17177 punct ),3
R13532 T17179 T17177 cc and,3
R13533 T17180 T17177 conj immunoprecipitation,3
R13534 T17181 T17182 punct (,IP
R13535 T17182 T17180 appos IP,immunoprecipitation
R13536 T17183 T17180 punct ),immunoprecipitation
R13537 T17184 T17195 nsubjpass samples,fractionated
R13538 T17185 T17186 punct (,lanes
R13539 T17186 T17184 parataxis lanes,samples
R13540 T17187 T17186 nummod 4,lanes
R13541 T17188 T17186 appos 11,lanes
R13542 T17189 T17186 punct ),lanes
R13543 T17190 T17184 prep from,samples
R13544 T17191 T17193 det the,lines
R13545 T17192 T17193 compound cell,lines
R13546 T17193 T17190 pobj lines,from
R13547 T17194 T17195 auxpass were,fractionated
R13548 T17195 T17195 ROOT fractionated,fractionated
R13549 T17196 T17195 agent by,fractionated
R13550 T17197 T17196 pobj SDS-PAGE,by
R13551 T17198 T17195 cc and,fractionated
R13552 T17199 T17195 conj evaluated,fractionated
R13553 T17200 T17199 agent by,evaluated
R13554 T17201 T17118 amod Western,blotting
R13555 T17202 T17118 prep with,blotting
R13556 T17203 T17202 pobj antibodies,with
R13557 T17204 T17199 prep to,evaluated
R13558 T17205 T17207 det the,tag
R13559 T17206 T17207 compound BP,tag
R13560 T17207 T17204 pobj tag,to
R13561 T17208 T17209 punct (,IKKγ
R13562 T17209 T17207 appos IKKγ,tag
R13563 T17210 T17209 punct ",",IKKγ
R13564 T17211 T17212 amod upper,panel
R13565 T17212 T17209 appos panel,IKKγ
R13566 T17213 T17209 punct ),IKKγ
R13567 T17214 T17207 cc and,tag
R13568 T17215 T17207 conj TRAF2,tag
R13569 T17216 T17195 punct .,fractionated
R13570 T17217 T17220 det The,sample
R13571 T17218 T17220 amod anti-CD40,sample
R13572 T17219 T17220 amod IP,sample
R13573 T17220 T17225 nsubjpass sample,treated
R13574 T17221 T17220 prep in,sample
R13575 T17222 T17221 pobj lane,in
R13576 T17223 T17222 nummod 11,lane
R13577 T17224 T17225 auxpass was,treated
R13578 T17225 T17235 ccomp treated,was
R13579 T17226 T17225 prep with,treated
R13580 T17227 T17228 amod λ,phosphatase
R13581 T17228 T17226 pobj phosphatase,with
R13582 T17229 T17235 punct ;,was
R13583 T17230 T17231 det the,sample
R13584 T17231 T17235 nsubj sample,was
R13585 T17232 T17231 prep in,sample
R13586 T17233 T17232 pobj lane,in
R13587 T17234 T17233 nummod 10,lane
R13588 T17235 T17235 ROOT was,was
R13589 T17236 T17235 attr mock-treated,was
R13590 T17237 T17235 punct .,was
R13591 T17238 T17239 compound Protein,samples
R13592 T17239 T17249 nsubjpass samples,fractionated
R13593 T17240 T17239 punct (,samples
R13594 T17241 T17242 acomp minus,those
R13595 T17242 T17239 appos those,samples
R13596 T17243 T17242 acl treated,those
R13597 T17244 T17243 prep with,treated
R13598 T17245 T17244 pobj phosphatase,with
R13599 T17246 T17239 punct ),samples
R136 T214 T215 compound engagement,results
R13600 T17247 T17249 auxpass were,fractionated
R13601 T17248 T17249 advmod also,fractionated
R13602 T17249 T17249 ROOT fractionated,fractionated
R13603 T17250 T17249 prep on,fractionated
R13604 T17251 T17253 det a,gel
R13605 T17252 T17253 amod separate,gel
R13606 T17253 T17250 pobj gel,on
R13607 T17254 T17253 punct (,gel
R13608 T17255 T17257 amod lower,concentration
R13609 T17256 T17257 compound acrylamide,concentration
R13610 T17257 T17253 appos concentration,gel
R13611 T17258 T17253 punct ),gel
R13612 T17259 T17249 prep for,fractionated
R13613 T17260 T17261 det the,evaluation
R13614 T17261 T17259 pobj evaluation,for
R13615 T17262 T17261 prep of,evaluation
R13616 T17263 T17262 pobj TRAF3,of
R13617 T17264 T17263 cc and,TRAF3
R13618 T17265 T17263 conj HOIP,TRAF3
R13619 T17266 T17269 punct (,panels
R13620 T17267 T17269 amod bottom,panels
R13621 T17268 T17269 nummod two,panels
R13622 T17269 T17265 appos panels,HOIP
R13623 T17270 T17265 punct ),HOIP
R13624 T17271 T17249 punct .,fractionated
R13625 T17272 T17273 amod Similar,results
R13626 T17273 T17275 nsubjpass results,obtained
R13627 T17274 T17275 auxpass were,obtained
R13628 T17275 T17275 ROOT obtained,obtained
R13629 T17276 T17275 prep in,obtained
R13630 T17277 T17279 nummod two,experiments
R13631 T17278 T17279 amod additional,experiments
R13632 T17279 T17276 pobj experiments,in
R13633 T17280 T17275 punct .,obtained
R137 T215 T215 ROOT results,results
R138 T216 T215 prep in,results
R139 T217 T218 det the,formation
R140 T218 T216 pobj formation,in
R141 T219 T218 prep of,formation
R142 T220 T222 det a,complex
R143 T221 T222 amod receptor-associated,complex
R144 T222 T219 pobj complex,of
R145 T223 T224 nsubj that,mediates
R146 T224 T222 relcl mediates,complex
R147 T225 T224 dobj activation,mediates
R148 T226 T225 prep of,activation
R149 T227 T226 pobj NF-κB,of
R150 T228 T227 punct ",",NF-κB
R151 T229 T231 amod stress-activated,kinases
R152 T230 T231 compound protein,kinases
R153 T231 T225 conj kinases,activation
R154 T232 T231 punct ",",kinases
R155 T233 T231 cc and,kinases
R156 T234 T236 amod other,molecules
R157 T235 T236 amod signaling,molecules
R158 T236 T231 conj molecules,kinases
R159 T237 T215 punct .,results
R160 T238 T250 advmod However,been
R161 T239 T250 punct ",",been
R162 T240 T241 det the,mechanisms
R163 T241 T250 nsubj mechanisms,been
R164 T242 T243 nsubj that,link
R165 T243 T241 relcl link,mechanisms
R166 T244 T243 dobj CD40,link
R167 T245 T243 prep to,link
R168 T246 T248 det these,events
R169 T247 T248 amod signaling,events
R170 T248 T245 pobj events,to
R171 T249 T250 aux have,been
R172 T250 T250 ROOT been,been
R173 T251 T253 advmod only,characterized
R174 T252 T253 advmod partially,characterized
R175 T253 T250 acomp characterized,been
R176 T254 T253 punct .,characterized
R177 T255 T256 amod Known,components
R178 T256 T262 nsubj components,include
R179 T257 T256 prep of,components
R180 T258 T259 det the,CD40
R181 T259 T257 pobj CD40,of
R182 T260 T256 acl signaling,components
R183 T261 T260 pcomp complex,signaling
R184 T262 T262 ROOT include,include
R185 T263 T262 dobj members,include
R186 T264 T263 prep of,members
R187 T265 T268 det the,factor
R188 T266 T268 nmod TNF,factor
R189 T267 T268 amod receptor-associated,factor
R190 T268 T264 pobj factor,of
R191 T269 T270 punct (,TRAF
R192 T270 T268 appos TRAF,factor
R193 T271 T270 punct ),TRAF
R194 T272 T263 conj family,members
R195 T273 T272 prep of,family
R196 T274 T273 pobj proteins,of
R197 T275 T262 punct .,include
R198 T276 T278 nsubj We,showed
R199 T277 T278 advmod previously,showed
R200 T278 T278 ROOT showed,showed
R201 T279 T285 mark that,mediates
R202 T280 T283 det the,member
R203 T281 T283 compound TRAF,member
R204 T282 T283 compound family,member
R205 T283 T285 nsubj member,mediates
R206 T284 T285 nummod TRAF2,mediates
R207 T285 T278 ccomp mediates,showed
R208 T286 T285 dobj recruitment,mediates
R209 T287 T286 prep of,recruitment
R210 T288 T289 amod HOIL-1L-interacting,protein
R211 T289 T287 pobj protein,of
R212 T290 T291 punct (,HOIP
R2125 T2880 T2880 ROOT Generation,Generation
R2126 T2881 T2880 prep of,Generation
R2127 T2882 T2883 compound HOIP-deficient,B
R2128 T2883 T2884 compound B,cells
R2129 T2884 T2881 pobj cells,of
R213 T291 T289 appos HOIP,protein
R2130 T2885 T2880 prep via,Generation
R2131 T2886 T2887 amod targeted,disruption
R2132 T2887 T2885 pobj disruption,via
R2133 T2888 T2887 prep of,disruption
R2134 T2889 T2888 pobj Rnf31,of
R2135 T2890 T2889 punct ",",Rnf31
R2136 T2891 T2892 det the,gene
R2137 T2892 T2889 appos gene,Rnf31
R2138 T2893 T2892 acl encoding,gene
R2139 T2894 T2893 dobj HOIP,encoding
R214 T292 T285 punct ),mediates
R2140 T2895 T2901 prep In,demonstrated
R2141 T2896 T2898 poss our,study
R2142 T2897 T2898 amod previous,study
R2143 T2898 T2895 pobj study,In
R2144 T2899 T2901 punct ",",demonstrated
R2145 T2900 T2901 nsubj we,demonstrated
R2146 T2901 T2901 ROOT demonstrated,demonstrated
R2147 T2902 T2905 mark that,recruited
R2148 T2903 T2905 nsubjpass HOIP,recruited
R2149 T2904 T2905 auxpass is,recruited
R215 T293 T285 prep to,mediates
R2150 T2905 T2901 ccomp recruited,demonstrated
R2151 T2906 T2905 prep to,recruited
R2152 T2907 T2908 det the,CD40
R2153 T2908 T2909 nsubj CD40,signaling
R2154 T2909 T2906 pcomp signaling,to
R2155 T2910 T2909 dobj complex,signaling
R2156 T2911 T2909 prep in,signaling
R2157 T2912 T2916 nummod two,lines
R2158 T2913 T2915 compound mouse,cell
R2159 T2914 T2915 compound B,cell
R216 T294 T296 det the,domain
R2160 T2915 T2916 compound cell,lines
R2161 T2916 T2911 pobj lines,in
R2162 T2917 T2906 pobj [,to
R2163 T2918 T2919 nummod 6,]
R2164 T2919 T2906 pobj ],to
R2165 T2920 T2901 punct .,demonstrated
R2166 T2921 T2922 amod Similar,results
R2167 T2922 T2924 nsubjpass results,obtained
R2168 T2923 T2924 auxpass were,obtained
R2169 T2924 T2924 ROOT obtained,obtained
R217 T295 T296 amod cytoplasmic,domain
R2170 T2925 T2924 prep with,obtained
R2171 T2926 T2927 compound mouse,splenocytes
R2172 T2927 T2925 pobj splenocytes,with
R2173 T2928 T2924 punct ",",obtained
R2174 T2929 T2924 advcl indicating,obtained
R2175 T2930 T2939 mark that,limited
R2176 T2931 T2932 det the,interaction
R2177 T2932 T2939 nsubjpass interaction,limited
R2178 T2933 T2932 prep of,interaction
R2179 T2934 T2933 pobj CD40,of
R218 T296 T293 pobj domain,to
R2180 T2935 T2932 prep with,interaction
R2181 T2936 T2935 pobj HOIP,with
R2182 T2937 T2939 auxpass is,limited
R2183 T2938 T2939 neg not,limited
R2184 T2939 T2929 ccomp limited,indicating
R2185 T2940 T2941 aux to,transformed
R2186 T2941 T2939 xcomp transformed,limited
R2187 T2942 T2943 compound B,cell
R2188 T2943 T2944 compound cell,lines
R2189 T2944 T2941 dobj lines,transformed
R219 T297 T296 prep of,domain
R2190 T2945 T2944 punct (,lines
R2191 T2946 T2947 amod unpublished,observations
R2192 T2947 T2944 appos observations,lines
R2193 T2948 T2944 punct ),lines
R2194 T2949 T2924 punct .,obtained
R2195 T2950 T2952 nsubj We,found
R2196 T2951 T2952 advmod also,found
R2197 T2952 T2952 ROOT found,found
R2198 T2953 T2960 mark that,was
R2199 T2954 T2955 det the,recruitment
R220 T298 T297 pobj CD40,of
R2200 T2955 T2960 nsubj recruitment,was
R2201 T2956 T2955 prep of,recruitment
R2202 T2957 T2956 pobj HOIP,of
R2203 T2958 T2955 prep to,recruitment
R2204 T2959 T2958 pobj CD40,to
R2205 T2960 T2952 ccomp was,found
R2206 T2961 T2960 acomp TRAF2-dependent,was
R2207 T2962 T2960 cc and,was
R2208 T2963 T2964 det that,overexpression
R2209 T2964 T2960 conj overexpression,was
R221 T299 T285 punct ",",mediates
R2210 T2965 T2964 prep of,overexpression
R2211 T2966 T2969 det a,mutant
R2212 T2967 T2969 amod truncated,mutant
R2213 T2968 T2969 compound HOIP,mutant
R2214 T2969 T2974 amod mutant,activation
R2215 T2970 T2971 advmod partially,inhibited
R2216 T2971 T2974 amod inhibited,activation
R2217 T2972 T2974 amod CD40-mediated,activation
R2218 T2973 T2974 compound NF-κB,activation
R2219 T2974 T2965 pobj activation,of
R222 T300 T285 acl suggesting,mediates
R2220 T2975 T2952 punct .,found
R2221 T2976 T2977 det These,results
R2222 T2977 T2978 nsubj results,support
R2223 T2978 T2978 ROOT support,support
R2224 T2979 T2980 det the,hypothesis
R2225 T2980 T2978 dobj hypothesis,support
R2226 T2981 T2983 mark that,plays
R2227 T2982 T2983 nsubj HOIP,plays
R2228 T2983 T2980 acl plays,hypothesis
R2229 T2984 T2985 det a,role
R223 T301 T303 mark that,has
R2230 T2985 T2983 dobj role,plays
R2231 T2986 T2985 prep in,role
R2232 T2987 T2989 nummod CD40,transduction
R2233 T2988 T2989 compound signal,transduction
R2234 T2989 T2986 pobj transduction,in
R2235 T2990 T2978 punct .,support
R2236 T2991 T2993 aux To,define
R2237 T2992 T2993 advmod further,define
R2238 T2993 T3007 advcl define,used
R2239 T2994 T2995 det the,role
R224 T302 T303 nsubj HOIP,has
R2240 T2995 T2993 dobj role,define
R2241 T2996 T2993 cc and,define
R2242 T2997 T2993 conj evaluate,define
R2243 T2998 T2999 det the,importance
R2244 T2999 T2997 dobj importance,evaluate
R2245 T3000 T2999 prep of,importance
R2246 T3001 T3000 pobj HOIP,of
R2247 T3002 T2997 prep in,evaluate
R2248 T3003 T3002 pobj CD40,in
R2249 T3004 T2997 advcl signaling,evaluate
R225 T303 T300 ccomp has,suggesting
R2250 T3005 T3007 punct ",",used
R2251 T3006 T3007 nsubj we,used
R2252 T3007 T3007 ROOT used,used
R2253 T3008 T3010 compound somatic,gene
R2254 T3009 T3010 compound cell,gene
R2255 T3010 T3007 dobj gene,used
R2256 T3011 T3010 acl targeting,gene
R2257 T3012 T3013 aux to,disrupt
R2258 T3013 T3011 xcomp disrupt,targeting
R2259 T3014 T3015 det the,gene
R226 T304 T305 det a,role
R2260 T3015 T3013 dobj gene,disrupt
R2261 T3016 T3015 acl encoding,gene
R2262 T3017 T3016 dobj HOIP,encoding
R2263 T3018 T3016 prep in,encoding
R2264 T3019 T3023 det the,line
R2265 T3020 T3022 compound mouse,cell
R2266 T3021 T3022 compound B,cell
R2267 T3022 T3023 compound cell,line
R2268 T3023 T3018 pobj line,in
R2269 T3024 T3026 nmod A20,J
R227 T305 T303 dobj role,has
R2270 T3025 T3026 nummod .2,J
R2271 T3026 T3023 appos J,line
R2272 T3027 T3007 punct .,used
R2273 T3028 T3030 det This,line
R2274 T3029 T3030 compound cell,line
R2275 T3030 T3032 nsubj line,been
R2276 T3031 T3032 aux has,been
R2277 T3032 T3032 ROOT been,been
R2278 T3033 T3034 advmod particularly,useful
R2279 T3034 T3032 acomp useful,been
R228 T306 T305 prep in,role
R2280 T3035 T3034 prep in,useful
R2281 T3036 T3037 det the,characterization
R2282 T3037 T3035 pobj characterization,in
R2283 T3038 T3037 prep of,characterization
R2284 T3039 T3038 pobj CD40,of
R2285 T3040 T3032 advcl signaling,been
R2286 T3041 T3040 dobj mechanisms,signaling
R2287 T3042 T3041 amod due,mechanisms
R2288 T3043 T3042 pcomp to,due
R2289 T3044 T3046 det the,ease
R229 T307 T310 det the,pathway
R2290 T3045 T3046 amod relative,ease
R2291 T3046 T3042 pobj ease,due
R2292 T3047 T3053 prep with,modified
R2293 T3048 T3047 pobj which,with
R2294 T3049 T3050 poss its,genome
R2295 T3050 T3053 nsubjpass genome,modified
R2296 T3051 T3053 aux can,modified
R2297 T3052 T3053 auxpass be,modified
R2298 T3053 T3046 relcl modified,ease
R2299 T3054 T3053 agent by,modified
R230 T308 T310 nmod CD40,pathway
R2300 T3055 T3057 nmod homologous,[
R2301 T3056 T3057 compound recombination,[
R2302 T3057 T3059 nmod [,]
R2303 T3058 T3059 nummod 7,]
R2304 T3059 T3054 pobj ],by
R2305 T3060 T3059 punct ",",]
R2306 T3061 T3063 nmod [,]
R2307 T3062 T3063 nummod 8,]
R2308 T3063 T3059 conj ],]
R2309 T3064 T3063 punct ",",]
R231 T309 T310 amod signaling,pathway
R2310 T3065 T3067 det [,]
R2311 T3066 T3067 nummod 9,]
R2312 T3067 T3063 appos ],]
R2313 T3068 T3032 punct .,been
R2314 T3069 T3070 nsubj We,used
R2315 T3070 T3070 ROOT used,used
R2316 T3071 T3073 det a,vector
R2317 T3072 T3073 compound targeting,vector
R2318 T3073 T3070 dobj vector,used
R2319 T3074 T3073 amod capable,vector
R232 T310 T306 pobj pathway,in
R2320 T3075 T3074 prep of,capable
R2321 T3076 T3075 pcomp undergoing,of
R2322 T3077 T3078 amod homologous,recombination
R2323 T3078 T3076 dobj recombination,undergoing
R2324 T3079 T3076 prep with,undergoing
R2325 T3080 T3079 pobj Rnf31,with
R2326 T3081 T3080 punct (,Rnf31
R2327 T3082 T3083 det the,gene
R2328 T3083 T3080 appos gene,Rnf31
R2329 T3084 T3083 acl encoding,gene
R233 T311 T278 punct .,showed
R2330 T3085 T3084 dobj HOIP,encoding
R2331 T3086 T3080 punct ),Rnf31
R2332 T3087 T3088 aux to,disrupt
R2333 T3088 T3076 advcl disrupt,undergoing
R2334 T3089 T3091 det the,sequence
R2335 T3090 T3091 compound coding,sequence
R2336 T3091 T3088 dobj sequence,disrupt
R2337 T3092 T3091 prep of,sequence
R2338 T3093 T3094 det the,gene
R2339 T3094 T3092 pobj gene,of
R234 T312 T313 aux To,determine
R2340 T3095 T3088 prep in,disrupt
R2341 T3096 T3095 pobj exon,in
R2342 T3097 T3096 nummod 5,exon
R2343 T3098 T3096 punct (,exon
R2344 T3099 T3100 compound Fig.,1A
R2345 T3100 T3096 appos 1A,exon
R2346 T3101 T3096 punct ),exon
R2347 T3102 T3070 punct .,used
R2348 T3103 T3115 prep Following,screened
R2349 T3104 T3103 pobj introduction,Following
R235 T313 T323 advcl determine,used
R2350 T3105 T3104 prep of,introduction
R2351 T3106 T3107 det the,vector
R2352 T3107 T3105 pobj vector,of
R2353 T3108 T3115 punct ",",screened
R2354 T3109 T3111 det the,clones
R2355 T3110 T3111 amod neomycin-resistant,clones
R2356 T3111 T3115 nsubjpass clones,screened
R2357 T3112 T3113 nsubj that,arose
R2358 T3113 T3111 relcl arose,clones
R2359 T3114 T3115 auxpass were,screened
R236 T314 T315 det the,role
R2360 T3115 T3115 ROOT screened,screened
R2361 T3116 T3115 agent by,screened
R2362 T3117 T3118 compound PCR,amplification
R2363 T3118 T3116 pobj amplification,by
R2364 T3119 T3118 prep of,amplification
R2365 T3120 T3121 amod genomic,DNA
R2366 T3121 T3119 pobj DNA,of
R2367 T3122 T3123 aux to,identify
R2368 T3123 T3115 xcomp identify,screened
R2369 T3124 T3123 dobj cells,identify
R237 T315 T313 dobj role,determine
R2370 T3125 T3124 acl containing,cells
R2371 T3126 T3129 det a,allele
R2372 T3127 T3129 amod disrupted,allele
R2373 T3128 T3129 compound Rnf31,allele
R2374 T3129 T3125 dobj allele,containing
R2375 T3130 T3115 punct .,screened
R2376 T3131 T3132 aux To,remove
R2377 T3132 T3149 advcl remove,transfected
R2378 T3133 T3137 det the,cassette
R2379 T3134 T3136 amod selectable,gene
R238 T316 T315 prep of,role
R2380 T3135 T3136 compound marker,gene
R2381 T3136 T3137 compound gene,cassette
R2382 T3137 T3132 dobj cassette,remove
R2383 T3138 T3132 prep from,remove
R2384 T3139 T3142 det the,allele
R2385 T3140 T3142 amod disrupted,allele
R2386 T3141 T3142 compound Rnf31,allele
R2387 T3142 T3138 pobj allele,from
R2388 T3143 T3149 punct ",",transfected
R2389 T3144 T3146 amod recombinant,lines
R239 T317 T316 pobj HOIP,of
R2390 T3145 T3146 compound cell,lines
R2391 T3146 T3149 nsubjpass lines,transfected
R2392 T3147 T3149 auxpass were,transfected
R2393 T3148 T3149 advmod transiently,transfected
R2394 T3149 T3149 ROOT transfected,transfected
R2395 T3150 T3149 prep with,transfected
R2396 T3151 T3152 det a,plasmid
R2397 T3152 T3150 pobj plasmid,with
R2398 T3153 T3154 nsubj that,encodes
R2399 T3154 T3152 relcl encodes,plasmid
R240 T318 T315 prep in,role
R2400 T3155 T3156 compound Cre,recombinase
R2401 T3156 T3154 dobj recombinase,encodes
R2402 T3157 T3149 punct .,transfected
R2403 T3158 T3159 det This,step
R2404 T3159 T3160 nsubj step,allowed
R2405 T3160 T3160 ROOT allowed,allowed
R2406 T3161 T3163 nsubj us,perform
R2407 T3162 T3163 aux to,perform
R2408 T3163 T3160 ccomp perform,allowed
R2409 T3164 T3166 det a,round
R241 T319 T318 pobj CD40,in
R2410 T3165 T3166 amod second,round
R2411 T3166 T3163 dobj round,perform
R2412 T3167 T3166 prep of,round
R2413 T3168 T3167 pobj targeting,of
R2414 T3169 T3168 cc and,targeting
R2415 T3170 T3171 compound drug,selection
R2416 T3171 T3168 conj selection,targeting
R2417 T3172 T3171 punct ",",selection
R2418 T3173 T3168 conj generating,targeting
R2419 T3174 T3173 dobj cells,generating
R242 T320 T315 acl signaling,role
R2420 T3175 T3182 prep in,disrupted
R2421 T3176 T3175 pobj which,in
R2422 T3177 T3178 det both,copies
R2423 T3178 T3182 nsubjpass copies,disrupted
R2424 T3179 T3178 prep of,copies
R2425 T3180 T3179 pobj Rnf31,of
R2426 T3181 T3182 auxpass were,disrupted
R2427 T3182 T3174 relcl disrupted,cells
R2428 T3183 T3160 punct .,allowed
R2429 T3184 T3188 nummod Two,lines
R243 T321 T323 punct ",",used
R2430 T3185 T3188 amod independent,lines
R2431 T3186 T3188 amod clonal,lines
R2432 T3187 T3188 compound cell,lines
R2433 T3188 T3190 nsubjpass lines,chosen
R2434 T3189 T3190 auxpass were,chosen
R2435 T3190 T3190 ROOT chosen,chosen
R2436 T3191 T3190 prep for,chosen
R2437 T3192 T3193 amod further,analysis
R2438 T3193 T3191 pobj analysis,for
R2439 T3194 T3190 punct .,chosen
R244 T322 T323 nsubj we,used
R2440 T3195 T3197 compound HOIP,expression
R2441 T3196 T3197 compound protein,expression
R2442 T3197 T3198 nsubj expression,was
R2443 T3198 T3198 ROOT was,was
R2444 T3199 T3198 acomp undetectable,was
R2445 T3200 T3199 prep in,undetectable
R2446 T3201 T3203 det both,lines
R2447 T3202 T3203 compound cell,lines
R2448 T3203 T3200 pobj lines,in
R2449 T3204 T3198 punct ",",was
R245 T323 T323 ROOT used,used
R2450 T3205 T3206 mark as,determined
R2451 T3206 T3198 advcl determined,was
R2452 T3207 T3206 agent by,determined
R2453 T3208 T3210 amod Western,analysis
R2454 T3209 T3210 compound blot,analysis
R2455 T3210 T3207 pobj analysis,by
R2456 T3211 T3210 prep of,analysis
R2457 T3212 T3213 compound cell,lysates
R2458 T3213 T3211 pobj lysates,of
R2459 T3214 T3210 punct (,analysis
R246 T324 T326 compound somatic,gene
R2460 T3215 T3216 compound Fig.,1B
R2461 T3216 T3210 appos 1B,analysis
R2462 T3217 T3210 punct ),analysis
R2463 T3218 T3198 punct ",",was
R2464 T3219 T3198 advcl demonstrating,was
R2465 T3220 T3224 mark that,was
R2466 T3221 T3223 det the,process
R2467 T3222 T3223 compound targeting,process
R2468 T3223 T3224 nsubj process,was
R2469 T3224 T3219 ccomp was,demonstrating
R247 T325 T326 compound cell,gene
R2470 T3225 T3224 acomp successful,was
R2471 T3226 T3198 punct .,was
R2472 T3227 T3239 prep In,referred
R2473 T3228 T3229 det the,text
R2474 T3229 T3227 pobj text,In
R2475 T3230 T3231 nsubj that,follows
R2476 T3231 T3229 relcl follows,text
R2477 T3232 T3239 punct ",",referred
R2478 T3233 T3237 det the,lines
R2479 T3234 T3237 nummod two,lines
R248 T326 T323 dobj gene,used
R2480 T3235 T3237 amod gene-targeted,lines
R2481 T3236 T3237 compound cell,lines
R2482 T3237 T3239 nsubjpass lines,referred
R2483 T3238 T3239 auxpass are,referred
R2484 T3239 T3239 ROOT referred,referred
R2485 T3240 T3239 prep to,referred
R2486 T3241 T3239 prep as,referred
R2487 T3242 T3243 amod HOIP-deficient,cells
R2488 T3243 T3241 pobj cells,as
R2489 T3244 T3239 punct .,referred
R249 T327 T326 acl targeting,gene
R2490 T3245 T3246 aux To,enable
R2491 T3246 T3274 advcl enable,transduced
R2492 T3247 T3246 dobj us,enable
R2493 T3248 T3249 aux to,confirm
R2494 T3249 T3246 xcomp confirm,enable
R2495 T3250 T3260 mark that,were
R2496 T3251 T3252 det any,signaling
R2497 T3252 T3260 nsubj signaling,were
R2498 T3253 T3252 cc or,signaling
R2499 T3254 T3255 amod functional,defects
R250 T328 T329 aux to,generate
R2500 T3255 T3256 nsubj defects,observed
R2501 T3256 T3252 conj observed,signaling
R2502 T3257 T3256 prep in,observed
R2503 T3258 T3259 amod HOIP-deficient,cells
R2504 T3259 T3257 pobj cells,in
R2505 T3260 T3249 ccomp were,confirm
R2506 T3261 T3260 acomp due,were
R2507 T3262 T3261 prep to,due
R2508 T3263 T3262 pobj disruption,to
R2509 T3264 T3263 prep of,disruption
R251 T329 T327 xcomp generate,targeting
R2510 T3265 T3267 det the,gene
R2511 T3266 T3267 compound Rnf31,gene
R2512 T3267 T3264 pobj gene,of
R2513 T3268 T3274 punct ",",transduced
R2514 T3269 T3272 det both,lines
R2515 T3270 T3272 amod HOIP-deficient,lines
R2516 T3271 T3272 compound cell,lines
R2517 T3272 T3274 nsubjpass lines,transduced
R2518 T3273 T3274 auxpass were,transduced
R2519 T3274 T3274 ROOT transduced,transduced
R252 T330 T333 compound mouse,lines
R2520 T3275 T3274 prep with,transduced
R2521 T3276 T3277 det a,retrovirus
R2522 T3277 T3278 nsubj retrovirus,encoding
R2523 T3278 T3281 amod encoding,HOIP
R2524 T3279 T3281 compound FLAG-tagged,HOIP
R2525 T3280 T3281 compound wild-type,HOIP
R2526 T3281 T3275 pobj HOIP,with
R2527 T3282 T3274 punct ",",transduced
R2528 T3283 T3284 advmod thus,restoring
R2529 T3284 T3274 advcl restoring,transduced
R253 T331 T332 compound B,cell
R2530 T3285 T3287 compound HOIP,expression
R2531 T3286 T3287 compound protein,expression
R2532 T3287 T3284 dobj expression,restoring
R2533 T3288 T3290 punct (,1B
R2534 T3289 T3290 compound Fig.,1B
R2535 T3290 T3284 appos 1B,restoring
R2536 T3291 T3290 punct ),1B
R2537 T3292 T3274 punct .,transduced
R2538 T3293 T3294 det These,cells
R2539 T3294 T3296 nsubjpass cells,referred
R254 T332 T333 compound cell,lines
R2540 T3295 T3296 auxpass are,referred
R2541 T3296 T3296 ROOT referred,referred
R2542 T3297 T3296 prep to,referred
R2543 T3298 T3296 prep as,referred
R2544 T3299 T3300 amod HOIP-reconstituted,cells
R2545 T3300 T3298 pobj cells,as
R2546 T3301 T3296 punct .,referred
R2547 T3302 T3313 prep In,compared
R2548 T3303 T3304 det the,experiments
R2549 T3304 T3302 pobj experiments,In
R255 T333 T329 dobj lines,generate
R2550 T3305 T3306 nsubj that,follow
R2551 T3306 T3304 relcl follow,experiments
R2552 T3307 T3313 punct ",",compared
R2553 T3308 T3313 nsubjpass responses,compared
R2554 T3309 T3308 prep by,responses
R2555 T3310 T3311 det these,cells
R2556 T3311 T3309 pobj cells,by
R2557 T3312 T3313 auxpass were,compared
R2558 T3313 T3313 ROOT compared,compared
R2559 T3314 T3313 prep to,compared
R256 T334 T323 oprd deficient,used
R2560 T3315 T3314 pobj those,to
R2561 T3316 T3315 prep of,those
R2562 T3317 T3320 compound A20,cells
R2563 T3318 T3319 compound .2,J
R2564 T3319 T3320 compound J,cells
R2565 T3320 T3316 pobj cells,of
R2566 T3321 T3320 cc and,cells
R2567 T3322 T3323 amod HOIP-deficient,cells
R2568 T3323 T3320 conj cells,cells
R2569 T3324 T3315 acl transduced,those
R257 T335 T334 prep in,deficient
R2570 T3325 T3324 prep with,transduced
R2571 T3326 T3328 det a,vector
R2572 T3327 T3328 amod retroviral,vector
R2573 T3328 T3325 pobj vector,with
R2574 T3329 T3328 punct (,vector
R2575 T3330 T3328 appos pMIP,vector
R2576 T3331 T3328 punct ),vector
R2577 T3332 T3315 acl lacking,those
R2578 T3333 T3335 det a,insert
R2579 T3334 T3335 compound cDNA,insert
R258 T336 T335 pobj HOIP,in
R2580 T3335 T3332 dobj insert,lacking
R2581 T3336 T3335 punct (,insert
R2582 T3337 T3338 amod empty,vector
R2583 T3338 T3335 appos vector,insert
R2584 T3339 T3335 punct ),insert
R2585 T3340 T3313 punct .,compared
R259 T337 T323 punct .,used
R260 T338 T339 nsubj We,found
R261 T339 T339 ROOT found,found
R262 T340 T353 mark that,were
R263 T341 T343 det the,upregulation
R264 T342 T343 amod CD40-induced,upregulation
R265 T343 T353 nsubj upregulation,were
R266 T344 T343 prep of,upregulation
R267 T345 T344 pobj CD80,of
R268 T346 T345 cc and,CD80
R269 T347 T343 conj activation,upregulation
R270 T348 T347 prep of,activation
R271 T349 T352 nmod germline,transcription
R272 T350 T352 compound immunoglobulin,transcription
R273 T351 T352 compound epsilon,transcription
R274 T352 T348 pobj transcription,of
R275 T353 T339 ccomp were,found
R276 T354 T353 acomp defective,were
R277 T355 T354 prep in,defective
R278 T356 T357 amod HOIP-deficient,cells
R279 T357 T355 pobj cells,in
R280 T358 T339 punct .,found
R281 T359 T361 nsubj We,found
R282 T360 T361 advmod also,found
R283 T361 T361 ROOT found,found
R284 T362 T372 mark that,impaired
R285 T363 T365 det the,activation
R286 T364 T365 amod CD40-mediated,activation
R287 T365 T372 nsubjpass activation,impaired
R288 T366 T365 prep of,activation
R289 T367 T366 pobj NF-κB,of
R290 T368 T367 cc and,NF-κB
R291 T369 T370 amod c-Jun,kinase
R292 T370 T367 conj kinase,NF-κB
R293 T371 T372 auxpass was,impaired
R294 T372 T361 ccomp impaired,found
R295 T373 T361 punct .,found
R296 T374 T384 nsubj Recruitment,was
R297 T375 T374 prep of,Recruitment
R298 T376 T378 compound IκB,proteins
R299 T377 T378 compound kinase,proteins
R300 T378 T375 pobj proteins,of
R301 T379 T374 prep to,Recruitment
R302 T380 T381 det the,CD40
R303 T381 T379 pobj CD40,to
R304 T382 T383 compound signaling,complex
R305 T383 T384 nsubj complex,was
R306 T384 T384 ROOT was,was
R307 T385 T384 acomp undetectable,was
R308 T386 T385 prep in,undetectable
R309 T387 T388 amod HOIP-deficient,cells
R310 T388 T386 pobj cells,in
R311 T389 T384 punct ",",was
R312 T390 T391 advmod potentially,explaining
R313 T391 T384 advcl explaining,was
R314 T392 T393 det the,defect
R315 T393 T391 dobj defect,explaining
R316 T394 T393 prep in,defect
R317 T395 T396 amod NF-κB,activation
R318 T396 T394 pobj activation,in
R319 T397 T384 punct .,was
R320 T398 T402 nsubj Restoration,reversed
R321 T399 T398 prep of,Restoration
R322 T400 T401 compound HOIP,expression
R323 T401 T399 pobj expression,of
R324 T402 T402 ROOT reversed,reversed
R3249 T4259 T4261 nsubjpass HOIP,required
R325 T403 T404 det the,defects
R3250 T4260 T4261 auxpass is,required
R3251 T4261 T4261 ROOT required,required
R3252 T4262 T4261 prep for,required
R3253 T4263 T4265 amod CD40-mediated,upregulation
R3254 T4264 T4265 compound CD80,upregulation
R3255 T4265 T4262 pobj upregulation,for
R3256 T4266 T4265 cc and,upregulation
R3257 T4267 T4265 conj activation,upregulation
R3258 T4268 T4267 prep of,activation
R3259 T4269 T4271 compound germline,transcription
R326 T404 T402 dobj defects,reversed
R3260 T4270 T4271 compound epsilon,transcription
R3261 T4271 T4268 pobj transcription,of
R3262 T4272 T4265 conj Engagement,upregulation
R3263 T4273 T4272 prep of,Engagement
R3264 T4274 T4273 pobj CD40,of
R3265 T4275 T4272 prep on,Engagement
R3266 T4276 T4277 compound B,cells
R3267 T4277 T4275 pobj cells,on
R3268 T4278 T4279 compound upregulates,expression
R3269 T4279 T4261 dobj expression,required
R327 T405 T404 prep in,defects
R3270 T4280 T4279 prep of,expression
R3271 T4281 T4282 compound CD80,[
R3272 T4282 T4280 pobj [,of
R3273 T4283 T4284 nummod 10,]
R3274 T4284 T4279 appos ],expression
R3275 T4285 T4284 punct ",",]
R3276 T4286 T4289 det a,protein
R3277 T4287 T4288 compound cell,surface
R3278 T4288 T4289 compound surface,protein
R3279 T4289 T4279 appos protein,expression
R328 T406 T407 amod cellular,activation
R3280 T4290 T4291 nsubj that,promotes
R3281 T4291 T4289 relcl promotes,protein
R3282 T4292 T4291 dobj activation,promotes
R3283 T4293 T4292 prep of,activation
R3284 T4294 T4295 compound T,cells
R3285 T4295 T4293 pobj cells,of
R3286 T4296 T4295 acl interacting,cells
R3287 T4297 T4296 prep with,interacting
R3288 T4298 T4299 compound B,cells
R3289 T4299 T4297 pobj cells,with
R329 T407 T405 pobj activation,in
R3290 T4300 T4299 cc and,cells
R3291 T4301 T4302 amod other,APC
R3292 T4302 T4299 conj APC,cells
R3293 T4303 T4261 punct .,required
R3294 T4304 T4305 aux To,begin
R3295 T4305 T4316 advcl begin,assayed
R3296 T4306 T4305 xcomp evaluating,begin
R3297 T4307 T4308 amod potential,contributions
R3298 T4308 T4306 dobj contributions,evaluating
R3299 T4309 T4308 prep of,contributions
R330 T408 T407 cc and,activation
R3300 T4310 T4309 pobj HOIP,of
R3301 T4311 T4306 prep to,evaluating
R3302 T4312 T4313 nsubj CD40,signaling
R3303 T4313 T4311 pcomp signaling,to
R3304 T4314 T4316 punct ",",assayed
R3305 T4315 T4316 nsubj we,assayed
R3306 T4316 T4316 ROOT assayed,assayed
R3307 T4317 T4318 det the,upregulation
R3308 T4318 T4316 dobj upregulation,assayed
R3309 T4319 T4318 prep of,upregulation
R331 T409 T407 conj signaling,activation
R3310 T4320 T4319 pobj CD80,of
R3311 T4321 T4318 acl induced,upregulation
R3312 T4322 T4321 agent by,induced
R3313 T4323 T4322 pobj engagement,by
R3314 T4324 T4323 prep of,engagement
R3315 T4325 T4324 pobj CD40,of
R3316 T4326 T4323 prep on,engagement
R3317 T4327 T4330 amod parental,J
R3318 T4328 T4330 nmod A20,J
R3319 T4329 T4330 nummod .2,J
R332 T410 T402 punct .,reversed
R3320 T4330 T4326 pobj J,on
R3321 T4331 T4330 punct ",",J
R3322 T4332 T4330 conj HOIP-deficient,J
R3323 T4333 T4332 punct ",",HOIP-deficient
R3324 T4334 T4332 cc and,HOIP-deficient
R3325 T4335 T4336 amod HOIP-reconstituted,cells
R3326 T4336 T4332 conj cells,HOIP-deficient
R3327 T4337 T4338 punct (,Fig.
R3328 T4338 T4336 parataxis Fig.,cells
R3329 T4339 T4338 nummod 2,Fig.
R333 T411 T412 det These,results
R3330 T4340 T4338 punct ),Fig.
R3331 T4341 T4316 punct .,assayed
R3332 T4342 T4356 prep Compared,reduced
R3333 T4343 T4342 prep to,Compared
R3334 T4344 T4347 det the,line
R3335 T4345 T4347 amod parental,line
R3336 T4346 T4347 compound cell,line
R3337 T4347 T4343 pobj line,to
R3338 T4348 T4347 punct ",",line
R3339 T4349 T4351 det the,upregulation
R334 T412 T413 nsubj results,reveal
R3340 T4350 T4351 amod CD40-stimulated,upregulation
R3341 T4351 T4356 nsubjpass upregulation,reduced
R3342 T4352 T4351 prep of,upregulation
R3343 T4353 T4352 pobj CD80,of
R3344 T4354 T4356 auxpass was,reduced
R3345 T4355 T4356 advmod dramatically,reduced
R3346 T4356 T4356 ROOT reduced,reduced
R3347 T4357 T4356 prep in,reduced
R3348 T4358 T4360 det all,cells
R3349 T4359 T4360 amod HOIP-deficient,cells
R335 T413 T413 ROOT reveal,reveal
R3350 T4360 T4357 pobj cells,in
R3351 T4361 T4356 conj tested,reduced
R3352 T4362 T4356 punct .,reduced
R3353 T4363 T4370 prep In,restored
R3354 T4364 T4363 pobj contrast,In
R3355 T4365 T4370 punct ",",restored
R3356 T4366 T4370 nsubjpass upregulation,restored
R3357 T4367 T4366 prep of,upregulation
R3358 T4368 T4367 pobj CD80,of
R3359 T4369 T4370 auxpass was,restored
R336 T414 T413 dobj HOIP,reveal
R3360 T4370 T4370 ROOT restored,restored
R3361 T4371 T4370 prep in,restored
R3362 T4372 T4373 amod HOIP-reconstituted,cells
R3363 T4373 T4371 pobj cells,in
R3364 T4374 T4370 punct ",",restored
R3365 T4375 T4370 advcl confirming,restored
R3366 T4376 T4386 mark that,was
R3367 T4377 T4378 det the,defect
R3368 T4378 T4386 nsubj defect,was
R3369 T4379 T4378 prep in,defect
R337 T415 T413 prep as,reveal
R3370 T4380 T4381 compound CD80,upregulation
R3371 T4381 T4379 pobj upregulation,in
R3372 T4382 T4381 prep in,upregulation
R3373 T4383 T4385 det the,cells
R3374 T4384 T4385 amod HOIP-deficient,cells
R3375 T4385 T4382 pobj cells,in
R3376 T4386 T4375 ccomp was,confirming
R3377 T4387 T4386 acomp due,was
R3378 T4388 T4387 prep to,due
R3379 T4389 T4390 det the,disruption
R338 T416 T418 det a,component
R3380 T4390 T4388 pobj disruption,to
R3381 T4391 T4390 prep of,disruption
R3382 T4392 T4391 pobj Rnf31,of
R3383 T4393 T4370 punct .,restored
R3384 T4394 T4395 nummod CD40,signals
R3385 T4395 T4401 nsubj signals,contribute
R3386 T4396 T4395 prep in,signals
R3387 T4397 T4398 compound B,cells
R3388 T4398 T4396 pobj cells,in
R3389 T4399 T4401 aux can,contribute
R339 T417 T418 amod key,component
R3390 T4400 T4401 advmod also,contribute
R3391 T4401 T4401 ROOT contribute,contribute
R3392 T4402 T4401 prep to,contribute
R3393 T4403 T4404 det the,activation
R3394 T4404 T4402 pobj activation,to
R3395 T4405 T4404 prep of,activation
R3396 T4406 T4407 compound DNA,recombination
R3397 T4407 T4405 pobj recombination,of
R3398 T4408 T4404 prep in,activation
R3399 T4409 T4413 det the,locus
R340 T418 T415 pobj component,as
R3400 T4410 T4413 compound immunoglobulin,locus
R3401 T4411 T4412 amod heavy,chain
R3402 T4412 T4413 compound chain,locus
R3403 T4413 T4408 pobj locus,in
R3404 T4414 T4416 compound [,]
R3405 T4415 T4416 compound 11,]
R3406 T4416 T4404 appos ],activation
R3407 T4417 T4416 punct ",",]
R3408 T4418 T4420 nmod [,]
R3409 T4419 T4420 nummod 12,]
R341 T419 T418 prep of,component
R3410 T4420 T4416 appos ],]
R3411 T4421 T4401 punct .,contribute
R3412 T4422 T4423 det This,process
R3413 T4423 T4436 nsubj process,allows
R3414 T4424 T4423 punct ",",process
R3415 T4425 T4423 acl known,process
R3416 T4426 T4425 prep as,known
R3417 T4427 T4429 amod immunoglobulin,switching
R3418 T4428 T4429 compound class,switching
R3419 T4429 T4426 pobj switching,as
R342 T420 T421 det the,CD40
R3420 T4430 T4429 punct (,switching
R3421 T4431 T4429 cc or,switching
R3422 T4432 T4433 compound isotype,switching
R3423 T4433 T4429 conj switching,switching
R3424 T4434 T4423 punct ),process
R3425 T4435 T4436 punct ",",allows
R3426 T4436 T4436 ROOT allows,allows
R3427 T4437 T4438 compound B,cells
R3428 T4438 T4436 dobj cells,allows
R3429 T4439 T4436 punct ",",allows
R343 T421 T419 pobj CD40,of
R3430 T4440 T4441 advmod initially,expressing
R3431 T4441 T4436 advcl expressing,allows
R3432 T4442 T4441 dobj IgM,expressing
R3433 T4443 T4436 punct ",",allows
R3434 T4444 T4445 aux to,switch
R3435 T4445 T4436 advcl switch,allows
R3436 T4446 T4445 prep to,switch
R3437 T4447 T4446 pobj IgA,to
R3438 T4448 T4447 punct ",",IgA
R3439 T4449 T4447 conj IgG,IgA
R344 T422 T413 advcl signaling,reveal
R3440 T4450 T4449 punct ",",IgG
R3441 T4451 T4449 cc or,IgG
R3442 T4452 T4449 conj IgE,IgG
R3443 T4453 T4445 prep depending,switch
R3444 T4454 T4453 prep on,depending
R3445 T4455 T4456 det the,class
R3446 T4456 T4454 pobj class,on
R3447 T4457 T4456 prep of,class
R3448 T4458 T4457 pobj antibody,of
R3449 T4459 T4460 advmod most,appropriate
R345 T423 T422 dobj pathway,signaling
R3450 T4460 T4456 amod appropriate,class
R3451 T4461 T4460 prep for,appropriate
R3452 T4462 T4465 det a,response
R3453 T4463 T4465 amod particular,response
R3454 T4464 T4465 amod immune,response
R3455 T4465 T4461 pobj response,for
R3456 T4466 T4436 punct .,allows
R3457 T4467 T4472 nsubjpass Switching,induced
R3458 T4468 T4467 prep to,Switching
R3459 T4469 T4468 pobj IgE,to
R346 T424 T413 punct .,reveal
R3460 T4470 T4472 aux can,induced
R3461 T4471 T4472 auxpass be,induced
R3462 T4472 T4472 ROOT induced,induced
R3463 T4473 T4472 agent by,induced
R3464 T4474 T4475 det the,combination
R3465 T4475 T4473 pobj combination,by
R3466 T4476 T4475 prep of,combination
R3467 T4477 T4480 nmod IL-4,signals
R3468 T4478 T4477 cc and,IL-4
R3469 T4479 T4480 amod CD40,signals
R3470 T4480 T4476 pobj signals,of
R3471 T4481 T4472 punct .,induced
R3472 T4482 T4484 det The,rearrangement
R3473 T4483 T4484 compound gene,rearrangement
R3474 T4484 T4490 nsubjpass rearrangement,preceded
R3475 T4485 T4484 amod necessary,rearrangement
R3476 T4486 T4485 prep for,necessary
R3477 T4487 T4488 amod IgE,production
R3478 T4488 T4486 pobj production,for
R3479 T4489 T4490 auxpass is,preceded
R3480 T4490 T4490 ROOT preceded,preceded
R3481 T4491 T4490 agent by,preceded
R3482 T4492 T4493 det the,production
R3483 T4493 T4491 pobj production,by
R3484 T4494 T4493 prep of,production
R3485 T4495 T4497 amod non-coding,transcripts
R3486 T4496 T4497 compound RNA,transcripts
R3487 T4497 T4494 pobj transcripts,of
R3488 T4498 T4497 prep from,transcripts
R3489 T4499 T4500 amod germline,sequences
R3490 T4500 T4498 pobj sequences,from
R3491 T4501 T4500 prep in,sequences
R3492 T4502 T4505 det the,chain
R3493 T4503 T4505 nmod IgE,chain
R3494 T4504 T4505 amod heavy,chain
R3495 T4505 T4501 pobj chain,in
R3496 T4506 T4491 pobj locus,by
R3497 T4507 T4509 nmod [,]
R3498 T4508 T4509 nummod 13,]
R3499 T4509 T4506 appos ],locus
R3500 T4510 T4490 punct .,preceded
R3501 T4511 T4512 aux To,determine
R3502 T4512 T4532 advcl determine,stimulated
R3503 T4513 T4516 mark whether,required
R3504 T4514 T4516 nsubjpass HOIP,required
R3505 T4515 T4516 auxpass is,required
R3506 T4516 T4512 ccomp required,determine
R3507 T4517 T4516 prep in,required
R3508 T4518 T4519 det the,initiation
R3509 T4519 T4517 pobj initiation,in
R3510 T4520 T4519 prep of,initiation
R3511 T4521 T4524 det the,process
R3512 T4522 T4524 compound class,process
R3513 T4523 T4524 compound switching,process
R3514 T4524 T4520 pobj process,of
R3515 T4525 T4532 punct ",",stimulated
R3516 T4526 T4530 det the,lines
R3517 T4527 T4530 amod relevant,lines
R3518 T4528 T4529 compound B,cell
R3519 T4529 T4530 compound cell,lines
R3520 T4530 T4532 nsubjpass lines,stimulated
R3521 T4531 T4532 auxpass were,stimulated
R3522 T4532 T4532 ROOT stimulated,stimulated
R3523 T4533 T4532 prep through,stimulated
R3524 T4534 T4533 pobj CD40,through
R3525 T4535 T4532 prep in,stimulated
R3526 T4536 T4537 det the,presence
R3527 T4537 T4535 pobj presence,in
R3528 T4538 T4537 prep of,presence
R3529 T4539 T4538 pobj IL-4,of
R3530 T4540 T4532 cc and,stimulated
R3531 T4541 T4532 conj tested,stimulated
R3532 T4542 T4541 prep for,tested
R3533 T4543 T4542 pobj production,for
R3534 T4544 T4543 prep of,production
R3535 T4545 T4546 compound germline,epsilon
R3536 T4546 T4544 pobj epsilon,of
R3537 T4547 T4548 punct (,GLε
R3538 T4548 T4546 appos GLε,epsilon
R3539 T4549 T4546 punct ),epsilon
R3540 T4550 T4542 pobj transcripts,for
R3541 T4551 T4552 punct (,Fig.
R3542 T4552 T4550 appos Fig.,transcripts
R3543 T4553 T4552 nummod 3,Fig.
R3544 T4554 T4552 punct ),Fig.
R3545 T4555 T4532 punct .,stimulated
R3546 T4556 T4558 amod Anti-CD40,stimulation
R3547 T4557 T4558 compound antibody,stimulation
R3548 T4558 T4564 nsubj stimulation,induced
R3549 T4559 T4558 advmod alone,stimulation
R3550 T4560 T4559 cc or,alone
R3551 T4561 T4559 conj together,alone
R3552 T4562 T4558 prep with,stimulation
R3553 T4563 T4562 pobj IL-4,with
R3554 T4564 T4564 ROOT induced,induced
R3555 T4565 T4566 compound GLε,transcription
R3556 T4566 T4564 dobj transcription,induced
R3557 T4567 T4566 prep in,transcription
R3558 T4568 T4572 amod parental,cells
R3559 T4569 T4572 compound A20,cells
R3560 T4570 T4571 compound .2,J
R3561 T4571 T4572 compound J,cells
R3562 T4572 T4567 pobj cells,in
R3563 T4573 T4574 mark as,expected
R3564 T4574 T4572 advcl expected,cells
R3565 T4575 T4564 punct .,induced
R3566 T4576 T4586 prep In,detected
R3567 T4577 T4576 pobj contrast,In
R3568 T4578 T4586 punct ",",detected
R3569 T4579 T4581 advmod only,levels
R3570 T4580 T4581 amod low,levels
R3571 T4581 T4586 nsubjpass levels,detected
R3572 T4582 T4581 prep of,levels
R3573 T4583 T4584 compound GLε,expression
R3574 T4584 T4582 pobj expression,of
R3575 T4585 T4586 auxpass were,detected
R3576 T4586 T4586 ROOT detected,detected
R3577 T4587 T4586 prep in,detected
R3578 T4588 T4589 amod HOIP-deficient,cells
R3579 T4589 T4587 pobj cells,in
R3580 T4590 T4586 conj stimulated,detected
R3581 T4591 T4590 prep with,stimulated
R3582 T4592 T4594 det either,antibody
R3583 T4593 T4594 amod anti-CD40,antibody
R3584 T4594 T4591 pobj antibody,with
R3585 T4595 T4594 advmod alone,antibody
R3586 T4596 T4594 cc or,antibody
R3587 T4597 T4594 conj anti-CD40,antibody
R3588 T4598 T4597 cc and,anti-CD40
R3589 T4599 T4597 conj IL-4,anti-CD40
R3590 T4600 T4586 punct .,detected
R3591 T4601 T4602 amod Normal,levels
R3592 T4602 T4607 nsubjpass levels,induced
R3593 T4603 T4602 prep of,levels
R3594 T4604 T4605 compound GLε,expression
R3595 T4605 T4603 pobj expression,of
R3596 T4606 T4607 auxpass were,induced
R3597 T4607 T4607 ROOT induced,induced
R3598 T4608 T4607 prep in,induced
R3599 T4609 T4610 amod HOIP-reconstituted,cells
R3600 T4610 T4608 pobj cells,in
R3601 T4611 T4607 conj stimulated,induced
R3602 T4612 T4611 prep with,stimulated
R3603 T4613 T4614 amod anti-CD40,antibody
R3604 T4614 T4612 pobj antibody,with
R3605 T4615 T4614 advmod alone,antibody
R3606 T4616 T4615 cc or,alone
R3607 T4617 T4618 advmod together,with
R3608 T4618 T4615 conj with,alone
R3609 T4619 T4618 pobj IL-4,with
R3610 T4620 T4607 punct .,induced
R3611 T4621 T4625 advmod Collectively,demonstrate
R3612 T4622 T4625 punct ",",demonstrate
R3613 T4623 T4624 det these,data
R3614 T4624 T4625 nsubj data,demonstrate
R3615 T4625 T4625 ROOT demonstrate,demonstrate
R3616 T4626 T4628 mark that,has
R3617 T4627 T4628 nsubj HOIP,has
R3618 T4628 T4625 ccomp has,demonstrate
R3619 T4629 T4631 det a,role
R3620 T4630 T4631 amod critical,role
R3621 T4631 T4628 dobj role,has
R3622 T4632 T4631 prep in,role
R3623 T4633 T4634 amod cellular,responses
R3624 T4634 T4632 pobj responses,in
R3625 T4635 T4634 prep to,responses
R3626 T4636 T4637 dep CD40,signaling
R3627 T4637 T4628 prep signaling,has
R3628 T4638 T4625 punct .,demonstrate
R4202 T5508 T5509 nsubj HOIP,mediates
R4203 T5509 T5509 ROOT mediates,mediates
R4204 T5510 T5511 amod CD40-stimulated,NF-κB
R4205 T5511 T5509 dobj NF-κB,mediates
R4206 T5512 T5511 cc and,NF-κB
R4207 T5513 T5514 compound JNK,activation
R4208 T5514 T5511 conj activation,NF-κB
R4209 T5515 T5516 det The,results
R4210 T5516 T5509 dobj results,mediates
R4211 T5517 T5516 acl described,results
R4212 T5518 T5517 prep above,described
R4213 T5519 T5518 pobj show,above
R4214 T5520 T5522 mark that,plays
R4215 T5521 T5522 nsubj HOIP,plays
R4216 T5522 T5519 ccomp plays,show
R4217 T5523 T5525 det an,role
R4218 T5524 T5525 amod important,role
R4219 T5525 T5522 dobj role,plays
R4220 T5526 T5525 prep in,role
R4221 T5527 T5529 amod CD40-mediated,functions
R4222 T5528 T5529 compound effector,functions
R4223 T5529 T5526 pobj functions,in
R4224 T5530 T5529 prep of,functions
R4225 T5531 T5532 compound B,cells
R4226 T5532 T5530 pobj cells,of
R4227 T5533 T5509 punct .,mediates
R4228 T5534 T5535 nsubj It,follows
R4229 T5535 T5535 ROOT follows,follows
R4230 T5536 T5538 mark that,is
R4231 T5537 T5538 nsubj HOIP,is
R4232 T5538 T5535 ccomp is,follows
R4233 T5539 T5538 acomp likely,is
R4234 T5540 T5542 det a,mediator
R4235 T5541 T5542 amod key,mediator
R4236 T5542 T5539 pobj mediator,likely
R4237 T5543 T5542 prep of,mediator
R4238 T5544 T5543 pobj CD40,of
R4239 T5545 T5542 acl signaling,mediator
R4240 T5546 T5535 punct .,follows
R4241 T5547 T5548 aux To,test
R4242 T5548 T5553 advcl test,stimulated
R4243 T5549 T5550 det this,hypothesis
R4244 T5550 T5548 dobj hypothesis,test
R4245 T5551 T5553 punct ",",stimulated
R4246 T5552 T5553 nsubj we,stimulated
R4247 T5553 T5553 ROOT stimulated,stimulated
R4248 T5554 T5556 nmod A20,J
R4249 T5555 T5556 nummod .2,J
R4250 T5556 T5553 dobj J,stimulated
R4251 T5557 T5556 cc and,J
R4252 T5558 T5559 amod HOIP-deficient,cells
R4253 T5559 T5556 conj cells,J
R4254 T5560 T5553 prep with,stimulated
R4255 T5561 T5560 pobj CD154,with
R4256 T5562 T5561 punct (,CD154
R4257 T5563 T5564 compound CD40,ligand
R4258 T5564 T5561 appos ligand,CD154
R4259 T5565 T5561 punct ),CD154
R4260 T5566 T5553 advcl expressed,stimulated
R4261 T5567 T5566 agent by,expressed
R4262 T5568 T5570 nummod HI5,cells
R4263 T5569 T5570 compound insect,cells
R4264 T5570 T5567 pobj cells,by
R4265 T5571 T5573 nmod [,]
R4266 T5572 T5573 nummod 7,]
R4267 T5573 T5553 npadvmod ],stimulated
R4268 T5574 T5573 punct ",",]
R4269 T5575 T5577 compound [,]
R4270 T5576 T5577 nummod 8,]
R4271 T5577 T5573 conj ],]
R4272 T5578 T5577 cc and,]
R4273 T5579 T5580 amod measured,activation
R4274 T5580 T5577 conj activation,]
R4275 T5581 T5580 prep of,activation
R4276 T5582 T5583 det the,NF-κB
R4277 T5583 T5581 pobj NF-κB,of
R4278 T5584 T5583 cc and,NF-κB
R4279 T5585 T5583 conj JNK,NF-κB
R4280 T5586 T5580 conj pathways,activation
R4281 T5587 T5586 punct ",",pathways
R4282 T5588 T5586 appos two,pathways
R4283 T5589 T5588 prep of,two
R4284 T5590 T5593 det the,regulators
R4285 T5591 T5593 amod major,regulators
R4286 T5592 T5593 amod transcriptional,regulators
R4287 T5593 T5589 pobj regulators,of
R4288 T5594 T5588 acl activated,two
R4289 T5595 T5594 agent by,activated
R4290 T5596 T5597 compound CD40,[
R4291 T5597 T5595 pobj [,by
R4292 T5598 T5599 nummod 4,]
R4293 T5599 T5597 appos ],[
R4294 T5600 T5553 punct .,stimulated
R4295 T5601 T5602 compound Cell-associated,CD154
R4296 T5602 T5604 nsubjpass CD154,used
R4297 T5603 T5604 auxpass was,used
R4298 T5604 T5604 ROOT used,used
R4299 T5605 T5604 prep as,used
R4300 T5606 T5607 det the,stimulus
R4301 T5607 T5605 pobj stimulus,as
R4302 T5608 T5607 prep in,stimulus
R4303 T5609 T5610 det these,experiments
R4304 T5610 T5608 pobj experiments,in
R4305 T5611 T5614 mark as,provides
R4306 T5612 T5614 nsubj it,provides
R4307 T5613 T5614 advmod typically,provides
R4308 T5614 T5604 advcl provides,used
R4309 T5615 T5616 advmod more,robust
R4310 T5616 T5614 advmod robust,provides
R4311 T5617 T5614 punct ",",provides
R4312 T5618 T5614 cc and,provides
R4313 T5619 T5622 advmod therefore,detected
R4314 T5620 T5621 advmod more,readily
R4315 T5621 T5622 advmod readily,detected
R4316 T5622 T5625 amod detected,signals
R4317 T5623 T5625 punct ",",signals
R4318 T5624 T5625 compound activation,signals
R4319 T5625 T5614 conj signals,provides
R4320 T5626 T5627 mark than,does
R4321 T5627 T5625 advcl does,signals
R4322 T5628 T5629 amod anti-CD40,antibody
R4323 T5629 T5627 nsubj antibody,does
R4324 T5630 T5604 punct .,used
R4325 T5631 T5638 nsubjpass Activation,initiated
R4326 T5632 T5631 prep of,Activation
R4327 T5633 T5636 det the,pathway
R4328 T5634 T5636 amod canonical,pathway
R4329 T5635 T5636 compound NF-κB,pathway
R4330 T5636 T5632 pobj pathway,of
R4331 T5637 T5638 auxpass is,initiated
R4332 T5638 T5638 ROOT initiated,initiated
R4333 T5639 T5638 prep with,initiated
R4334 T5640 T5641 det the,phosphorylation
R4335 T5641 T5639 pobj phosphorylation,with
R4336 T5642 T5641 prep of,phosphorylation
R4337 T5643 T5644 compound IκB,proteins
R4338 T5644 T5642 pobj proteins,of
R4339 T5645 T5641 prep by,phosphorylation
R4340 T5646 T5649 det the,complex
R4341 T5647 T5649 nmod IκB,complex
R4342 T5648 T5649 compound kinase,complex
R4343 T5649 T5645 pobj complex,by
R4344 T5650 T5651 punct (,IKK
R4345 T5651 T5649 appos IKK,complex
R4346 T5652 T5651 punct ),IKK
R4347 T5653 T5638 punct .,initiated
R4348 T5654 T5660 prep In,are
R4349 T5655 T5656 amod resting,cells
R4350 T5656 T5654 pobj cells,In
R4351 T5657 T5660 punct ",",are
R4352 T5658 T5659 compound IκB,proteins
R4353 T5659 T5660 nsubj proteins,are
R4354 T5660 T5660 ROOT are,are
R4355 T5661 T5660 acomp responsible,are
R4356 T5662 T5661 prep for,responsible
R4357 T5663 T5665 compound sequestering,subunits
R4358 T5664 T5665 compound NF-κB,subunits
R4359 T5665 T5662 pobj subunits,for
R4360 T5666 T5665 prep in,subunits
R4361 T5667 T5668 det the,cytoplasm
R4362 T5668 T5666 pobj cytoplasm,in
R4363 T5669 T5660 punct .,are
R4364 T5670 T5670 ROOT Phosphorylation,Phosphorylation
R4365 T5671 T5670 prep by,Phosphorylation
R4366 T5672 T5675 det the,targets
R4367 T5673 T5675 nmod IKK,targets
R4368 T5674 T5675 amod complex,targets
R4369 T5675 T5671 pobj targets,by
R4370 T5676 T5677 amod IκB,proteins
R4371 T5677 T5670 conj proteins,Phosphorylation
R4372 T5678 T5677 prep for,proteins
R4373 T5679 T5678 pobj ubiquitination,for
R4374 T5680 T5679 cc and,ubiquitination
R4375 T5681 T5679 conj degradation,ubiquitination
R4376 T5682 T5677 punct ",",proteins
R4377 T5683 T5670 acl allowing,Phosphorylation
R4378 T5684 T5686 nsubj NF-κB,enter
R4379 T5685 T5686 aux to,enter
R4380 T5686 T5683 ccomp enter,allowing
R4381 T5687 T5688 det the,nucleus
R4382 T5688 T5686 dobj nucleus,enter
R4383 T5689 T5686 cc and,enter
R4384 T5690 T5686 conj activate,enter
R4385 T5691 T5692 compound gene,expression
R4386 T5692 T5690 dobj expression,activate
R4387 T5693 T5670 punct .,Phosphorylation
R4388 T5694 T5695 amod CD40-mediated,phosphorylation
R4389 T5695 T5705 nsubjpass phosphorylation,impaired
R4390 T5696 T5695 cc and,phosphorylation
R4391 T5697 T5695 conj degradation,phosphorylation
R4392 T5698 T5697 prep of,degradation
R4393 T5699 T5698 pobj IκBα,of
R4394 T5700 T5697 prep in,degradation
R4395 T5701 T5702 amod HOIP-deficient,cells
R4396 T5702 T5700 pobj cells,in
R4397 T5703 T5705 auxpass was,impaired
R4398 T5704 T5705 advmod dramatically,impaired
R4399 T5705 T5705 ROOT impaired,impaired
R4400 T5706 T5705 oprd relative,impaired
R4401 T5707 T5706 prep to,relative
R4402 T5708 T5709 nsubj that,observed
R4403 T5709 T5707 pobj observed,to
R4404 T5710 T5709 prep in,observed
R4405 T5711 T5715 amod parental,cells
R4406 T5712 T5715 compound A20,cells
R4407 T5713 T5714 compound .2,J
R4408 T5714 T5715 compound J,cells
R4409 T5715 T5710 pobj cells,in
R4410 T5716 T5717 punct (,Fig.
R4411 T5717 T5719 nmod Fig.,)
R4412 T5718 T5717 nummod 4,Fig.
R4413 T5719 T5705 punct ),impaired
R4414 T5720 T5705 punct .,impaired
R4415 T5721 T5723 nsubj We,assayed
R4416 T5722 T5723 advmod also,assayed
R4417 T5723 T5723 ROOT assayed,assayed
R4418 T5724 T5723 dobj activation,assayed
R4419 T5725 T5724 prep of,activation
R4420 T5726 T5728 det the,protein
R4421 T5727 T5728 amod stress-activated,protein
R4422 T5728 T5725 pobj protein,of
R4423 T5729 T5730 compound kinase,JNK
R4424 T5730 T5728 appos JNK,protein
R4425 T5731 T5723 prep in,assayed
R4426 T5732 T5731 pobj response,in
R4427 T5733 T5732 prep to,response
R4428 T5734 T5735 nummod CD40,engagement
R4429 T5735 T5733 pobj engagement,to
R4430 T5736 T5738 punct (,4
R4431 T5737 T5738 nmod Fig.,4
R4432 T5738 T5735 appos 4,engagement
R4433 T5739 T5738 punct ),4
R4434 T5740 T5723 punct .,assayed
R4435 T5741 T5743 amod CD40-mediated,activation
R4436 T5742 T5743 compound JNK,activation
R4437 T5743 T5748 nsubjpass activation,impaired
R4438 T5744 T5743 prep in,activation
R4439 T5745 T5746 amod HOIP-deficient,cells
R4440 T5746 T5744 pobj cells,in
R4441 T5747 T5748 auxpass was,impaired
R4442 T5748 T5748 ROOT impaired,impaired
R4443 T5749 T5750 mark as,measured
R4444 T5750 T5748 advcl measured,impaired
R4445 T5751 T5750 agent by,measured
R4446 T5752 T5751 pobj phosphorylation,by
R4447 T5753 T5752 prep of,phosphorylation
R4448 T5754 T5753 pobj Thr183,of
R4449 T5755 T5754 cc and,Thr183
R4450 T5756 T5754 conj Tyr185,Thr183
R4451 T5757 T5752 prep in,phosphorylation
R4452 T5758 T5757 pobj JNK,in
R4453 T5759 T5748 punct .,impaired
R4454 T5760 T5761 amod CD40-induced,activation
R4455 T5761 T5769 nsubj activation,was
R4456 T5762 T5761 prep of,activation
R4457 T5763 T5762 pobj NF-κB,of
R4458 T5764 T5763 cc and,NF-κB
R4459 T5765 T5763 conj JNK,NF-κB
R4460 T5766 T5761 prep in,activation
R4461 T5767 T5768 amod HOIP-reconstituted,cells
R4462 T5768 T5766 pobj cells,in
R4463 T5769 T5769 ROOT was,was
R4464 T5770 T5769 acomp normal,was
R4465 T5771 T5769 punct ",",was
R4466 T5772 T5769 advcl demonstrating,was
R4467 T5773 T5776 mark that,observed
R4468 T5774 T5775 det the,defects
R4469 T5775 T5776 nsubj defects,observed
R4470 T5776 T5772 ccomp observed,demonstrating
R4471 T5777 T5776 prep in,observed
R4472 T5778 T5779 amod gene-deficient,cells
R4473 T5779 T5777 pobj cells,in
R4474 T5780 T5769 conj were,was
R4475 T5781 T5780 acomp due,were
R4476 T5782 T5781 prep to,due
R4477 T5783 T5784 det the,absence
R4478 T5784 T5782 pobj absence,to
R4479 T5785 T5784 prep of,absence
R4480 T5786 T5787 compound HOIP,expression
R4481 T5787 T5785 pobj expression,of
R4482 T5788 T5769 punct .,was
R5088 T6730 T6731 nsubj HOIP,is
R5089 T6731 T6731 ROOT is,is
R5090 T6732 T6731 acomp necessary,is
R5091 T6733 T6742 mark for,marked
R5092 T6734 T6742 nsubj association,marked
R5093 T6735 T6734 prep of,association
R5094 T6736 T6738 det the,complex
R5095 T6737 T6738 compound "IκB kinase (IKK) complex with CD40 was undetectable in HOIP-deficient cells. Together, our results indicate that HOIP plays a critical role in the activation of signaling pathways that regulate cellular responses to CD40 engagement. Results Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6]. Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations). We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation. These results support the hypothesis that HOIP plays a role in CD40 signal transduction. To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J. This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9]. We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A). Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele. To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase. This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted. Two independent clonal cell lines were chosen for further analysis. HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful. In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells. 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting. (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown. Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination. Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence. A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place. LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence. The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones. A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+). HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown. Approximate molecular weight of HOIP is 120 kD. To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B). These cells are referred to as HOIP-reconstituted cells. In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector). HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC. To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2). Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested. In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31. 10.1371/journal.pone.0023061.g002 Figure 2 CD40-mediated CD80 upregulation is defective in HOIP-deficient cells. Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody). Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not. Similar results were obtained in a second experiment. CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12]. This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response. Switching to IgE can be induced by the combination of IL-4 and CD40 signals. The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13]. To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3). Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected. In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4. Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4. Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling. 10.1371/journal.pone.0023061.g003 Figure 3 GLε transcription is defective in CD40-stimulated HOIP-deficient cells. A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4. RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts. Results were normalized to the levels of Hprt1 transcripts in each sample. Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values). Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone. HOIP mediates CD40-stimulated NF-κB and JNK activation The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells. It follows that HOIP is likely a key mediator of CD40 signaling. To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4]. Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody. Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK). In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm. Phosphorylation by the IKK",complex
R5096 T6738 T6735 pobj complex,of
R5097 T6739 T6734 prep with,association
R5098 T6740 T6739 pobj CD40,with
R5099 T6741 T6742 nsubj The,marked
R5100 T6742 T6731 advcl marked,is
R5101 T6743 T6742 dobj defects,marked
R5102 T6744 T6743 prep in,defects
R5103 T6745 T6747 amod CD40-mediated,activation
R5104 T6746 T6747 compound cell,activation
R5105 T6747 T6744 pobj activation,in
R5106 T6748 T6742 cc and,marked
R5107 T6749 T6754 nsubj signaling,suggested
R5108 T6750 T6749 acl displayed,signaling
R5109 T6751 T6750 agent by,displayed
R5110 T6752 T6753 amod HOIP-deficient,cells
R5111 T6753 T6751 pobj cells,by
R5112 T6754 T6742 conj suggested,marked
R5113 T6755 T6757 mark that,mediates
R5114 T6756 T6757 nsubj HOIP,mediates
R5115 T6757 T6754 ccomp mediates,suggested
R5116 T6758 T6757 dobj recruitment,mediates
R5117 T6759 T6758 prep of,recruitment
R5118 T6760 T6761 amod critical,components
R5119 T6761 T6759 pobj components,of
R5120 T6762 T6761 prep of,components
R5121 T6763 T6766 det the,apparatus
R5122 T6764 T6766 nmod CD40,apparatus
R5123 T6765 T6766 amod signaling,apparatus
R5124 T6766 T6762 pobj apparatus,of
R5125 T6767 T6758 prep to,recruitment
R5126 T6768 T6769 det the,receptor
R5127 T6769 T6767 pobj receptor,to
R5128 T6770 T6754 punct .,suggested
R5129 T6771 T6774 advmod Previously,demonstrated
R5130 T6772 T6774 punct ",",demonstrated
R5131 T6773 T6774 nsubj we,demonstrated
R5132 T6774 T6774 ROOT demonstrated,demonstrated
R5133 T6775 T6778 mark that,recruited
R5134 T6776 T6778 nsubjpass HOIP,recruited
R5135 T6777 T6778 auxpass is,recruited
R5136 T6778 T6774 ccomp recruited,demonstrated
R5137 T6779 T6778 prep to,recruited
R5138 T6780 T6781 det the,CD40
R5139 T6781 T6782 nsubj CD40,signaling
R5140 T6782 T6779 pcomp signaling,to
R5141 T6783 T6782 dobj complex,signaling
R5142 T6784 T6782 prep in,signaling
R5143 T6785 T6787 det a,manner
R5144 T6786 T6787 amod TRAF2-dependent,manner
R5145 T6787 T6784 pobj manner,in
R5146 T6788 T6774 punct ",",demonstrated
R5147 T6789 T6774 advcl suggesting,demonstrated
R5148 T6790 T6792 mark that,functions
R5149 T6791 T6792 compound HOIP,functions
R5150 T6792 T6789 ccomp functions,suggesting
R5151 T6793 T6792 amod downstream,functions
R5152 T6794 T6793 prep of,downstream
R5153 T6795 T6798 nummod TRAF2,]
R5154 T6796 T6798 nmod [,]
R5155 T6797 T6796 nummod 6,[
R5156 T6798 T6794 pobj ],of
R5157 T6799 T6774 punct .,demonstrated
R5158 T6800 T6803 nsubj Studies,suggest
R5159 T6801 T6800 prep by,Studies
R5160 T6802 T6801 pobj others,by
R5161 T6803 T6803 ROOT suggest,suggest
R5162 T6804 T6808 mark that,cIAP1
R5163 T6805 T6807 det the,proteins
R5164 T6806 T6807 amod TRAF2-associated,proteins
R5165 T6807 T6808 nsubj proteins,cIAP1
R5166 T6808 T6803 ccomp cIAP1,suggest
R5167 T6809 T6808 cc and,cIAP1
R5168 T6810 T6808 conj cIAP2,cIAP1
R5169 T6811 T6803 conj play,suggest
R5170 T6812 T6813 det a,role
R5171 T6813 T6811 dobj role,play
R5172 T6814 T6813 prep in,role
R5173 T6815 T6816 det the,recruitment
R5174 T6816 T6814 pobj recruitment,in
R5175 T6817 T6816 prep of,recruitment
R5176 T6818 T6817 pobj HOIP,of
R5177 T6819 T6813 prep to,role
R5178 T6820 T6819 pobj TNFR1,to
R5179 T6821 T6820 cc and,TNFR1
R5180 T6822 T6820 conj CD40,TNFR1
R5181 T6823 T6825 nmod [,]
R5182 T6824 T6825 nummod 14,]
R5183 T6825 T6819 pobj ],to
R5184 T6826 T6803 punct .,suggest
R5185 T6827 T6830 advmod Therefore,determined
R5186 T6828 T6830 punct ",",determined
R5187 T6829 T6830 nsubj we,determined
R5188 T6830 T6830 ROOT determined,determined
R5189 T6831 T6844 mark whether,altered
R5190 T6832 T6833 det the,association
R5191 T6833 T6844 nsubjpass association,altered
R5192 T6834 T6833 prep of,association
R5193 T6835 T6834 pobj HOIP,of
R5194 T6836 T6833 prep with,association
R5195 T6837 T6836 pobj CD40,with
R5196 T6838 T6833 prep in,association
R5197 T6839 T6838 pobj A20,in
R5198 T6840 T6841 compound .2,J
R5199 T6841 T6842 compound J,cells
R5200 T6842 T6844 nsubjpass cells,altered
R5201 T6843 T6844 auxpass was,altered
R5202 T6844 T6830 ccomp altered,determined
R5203 T6845 T6844 agent by,altered
R5204 T6846 T6845 pobj treatment,by
R5205 T6847 T6846 prep of,treatment
R5206 T6848 T6847 pobj cells,of
R5207 T6849 T6844 prep with,altered
R5208 T6850 T6851 det an,inhibitor
R5209 T6851 T6849 pobj inhibitor,with
R5210 T6852 T6851 prep of,inhibitor
R5211 T6853 T6854 amod cIAP,activity
R5212 T6854 T6852 pobj activity,of
R5213 T6855 T6851 punct ",",inhibitor
R5214 T6856 T6858 det a,peptide
R5215 T6857 T6858 amod membrane-permeable,peptide
R5216 T6858 T6851 appos peptide,inhibitor
R5217 T6859 T6858 acl derived,peptide
R5218 T6860 T6859 prep from,derived
R5219 T6861 T6863 det the,regulator
R5220 T6862 T6863 compound apoptosis,regulator
R5221 T6863 T6860 pobj regulator,from
R5222 T6864 T6865 compound SMAC,[
R5223 T6865 T6863 appos [,regulator
R5224 T6866 T6867 nummod 15,]
R5225 T6867 T6863 appos ],regulator
R5226 T6868 T6830 punct .,determined
R5227 T6869 T6870 nsubj We,found
R5228 T6870 T6870 ROOT found,found
R5229 T6871 T6880 mark that,reduced
R5230 T6872 T6880 nsubj pretreatment,reduced
R5231 T6873 T6872 prep of,pretreatment
R5232 T6874 T6873 pobj cells,of
R5233 T6875 T6872 prep with,pretreatment
R5234 T6876 T6878 det the,peptide
R5235 T6877 T6878 compound SMAC,peptide
R5236 T6878 T6875 pobj peptide,with
R5237 T6879 T6880 advmod dramatically,reduced
R5238 T6880 T6870 ccomp reduced,found
R5239 T6881 T6882 det the,amount
R5240 T6882 T6880 dobj amount,reduced
R5241 T6883 T6882 prep of,amount
R5242 T6884 T6883 pobj cIAP1,of
R5243 T6885 T6882 acl associated,amount
R5244 T6886 T6885 prep with,associated
R5245 T6887 T6888 det the,CD40
R5246 T6888 T6889 nsubj CD40,signaling
R5247 T6889 T6886 pcomp signaling,with
R5248 T6890 T6889 ccomp complex,signaling
R5249 T6891 T6890 prep in,complex
R5250 T6892 T6891 pobj cells,in
R5251 T6893 T6892 acl stimulated,cells
R5252 T6894 T6893 prep with,stimulated
R5253 T6895 T6897 amod anti-CD40,beads
R5254 T6896 T6897 amod antibody-coated,beads
R5255 T6897 T6894 pobj beads,with
R5256 T6898 T6897 punct (,beads
R5257 T6899 T6900 compound Fig.,5A
R5258 T6900 T6897 appos 5A,beads
R5259 T6901 T6897 punct ),beads
R5260 T6902 T6870 punct .,found
R5261 T6903 T6905 compound SMAC,treatment
R5262 T6904 T6905 compound peptide,treatment
R5263 T6905 T6907 nsubj treatment,resulted
R5264 T6906 T6907 advmod also,resulted
R5265 T6907 T6907 ROOT resulted,resulted
R5266 T6908 T6907 prep in,resulted
R5267 T6909 T6913 det a,decrease
R5268 T6910 T6913 amod slight,decrease
R5269 T6911 T6910 cc but,slight
R5270 T6912 T6910 conj reproducible,slight
R5271 T6913 T6908 pobj decrease,in
R5272 T6914 T6913 prep in,decrease
R5273 T6915 T6916 det the,amount
R5274 T6916 T6914 pobj amount,in
R5275 T6917 T6916 prep of,amount
R5276 T6918 T6921 det the,form
R5277 T6919 T6921 amod major,form
R5278 T6920 T6921 compound HOIP,form
R5279 T6921 T6917 pobj form,of
R5280 T6922 T6921 acl recovered,form
R5281 T6923 T6922 agent by,recovered
R5282 T6924 T6925 compound CD40,immunoprecipitation
R5283 T6925 T6923 pobj immunoprecipitation,by
R5284 T6926 T6907 punct ",",resulted
R5285 T6927 T6907 prep along,resulted
R5286 T6928 T6927 prep with,along
R5287 T6929 T6931 det an,increase
R5288 T6930 T6931 amod apparent,increase
R5289 T6931 T6928 pobj increase,with
R5290 T6932 T6931 prep in,increase
R5291 T6933 T6936 amod higher,species
R5292 T6934 T6936 amod molecular,species
R5293 T6935 T6936 compound weight,species
R5294 T6936 T6932 pobj species,in
R5295 T6937 T6936 acl recognized,species
R5296 T6938 T6937 agent by,recognized
R5297 T6939 T6940 amod anti-HOIP,antibody
R5298 T6940 T6938 pobj antibody,by
R5299 T6941 T6907 punct .,resulted
R5300 T6942 T6952 prep In,alter
R5301 T6943 T6942 pobj contrast,In
R5302 T6944 T6952 punct ",",alter
R5303 T6945 T6952 nsubj treatment,alter
R5304 T6946 T6945 prep with,treatment
R5305 T6947 T6949 det the,peptide
R5306 T6948 T6949 compound SMAC,peptide
R5307 T6949 T6946 pobj peptide,with
R5308 T6950 T6952 aux did,alter
R5309 T6951 T6952 neg not,alter
R5310 T6952 T6952 ROOT alter,alter
R5311 T6953 T6954 det the,amount
R5312 T6954 T6952 dobj amount,alter
R5313 T6955 T6954 cc or,amount
R5314 T6956 T6957 amod molecular,weight
R5315 T6957 T6954 conj weight,amount
R5316 T6958 T6954 prep of,amount
R5317 T6959 T6960 compound HOIP,present
R5318 T6960 T6958 pobj present,of
R5319 T6961 T6960 prep in,present
R5320 T6962 T6963 compound cell,lysates
R5321 T6963 T6961 pobj lysates,in
R5322 T6964 T6952 punct .,alter
R5323 T6965 T6966 det These,data
R5324 T6966 T6967 nsubj data,suggest
R5325 T6967 T6967 ROOT suggest,suggest
R5326 T6968 T6973 mark that,alters
R5327 T6969 T6971 compound SMAC,treatment
R5328 T6970 T6971 compound peptide,treatment
R5329 T6971 T6973 nsubj treatment,alters
R5330 T6972 T6973 advmod specifically,alters
R5331 T6973 T6967 ccomp alters,suggest
R5332 T6974 T6975 det the,characteristics
R5333 T6975 T6973 dobj characteristics,alters
R5334 T6976 T6975 prep of,characteristics
R5335 T6977 T6978 compound CD40-associated,HOIP
R5336 T6978 T6976 pobj HOIP,of
R5337 T6979 T6980 advmod rather,than
R5338 T6980 T6975 cc than,characteristics
R5339 T6981 T6984 det the,pool
R5340 T6982 T6984 amod entire,pool
R5341 T6983 T6984 amod cellular,pool
R5342 T6984 T6980 pobj pool,than
R5343 T6985 T6984 prep of,pool
R5344 T6986 T6987 det this,protein
R5345 T6987 T6985 pobj protein,of
R5346 T6988 T6967 punct .,suggest
R5347 T6989 T6993 advmod Together,support
R5348 T6990 T6993 punct ",",support
R5349 T6991 T6992 det these,results
R5350 T6992 T6993 nsubj results,support
R5351 T6993 T6993 ROOT support,support
R5352 T6994 T6995 det the,idea
R5353 T6995 T6993 dobj idea,support
R5354 T6996 T7000 mark that,influence
R5355 T6997 T6999 det the,proteins
R5356 T6998 T6999 compound cIAP,proteins
R5357 T6999 T7000 nsubj proteins,influence
R5358 T7000 T6995 acl influence,idea
R5359 T7001 T7002 det the,recruitment
R5360 T7002 T7000 dobj recruitment,influence
R5361 T7003 T7002 cc and,recruitment
R5362 T7004 T7006 amod post-translational,state
R5363 T7005 T7006 compound modification,state
R5364 T7006 T7002 conj state,recruitment
R5365 T7007 T7002 prep of,recruitment
R5366 T7008 T7009 compound CD40-associated,HOIP
R5367 T7009 T7007 pobj HOIP,of
R5368 T7010 T6993 punct .,support
R5369 T7011 T7012 aux To,test
R5370 T7012 T7031 advcl test,immunoprecipitated
R5371 T7013 T7014 det the,possibility
R5372 T7014 T7012 dobj possibility,test
R5373 T7015 T7017 mark that,is
R5374 T7016 T7017 nsubj HOIP,is
R5375 T7017 T7014 acl is,possibility
R5376 T7018 T7017 acomp responsible,is
R5377 T7019 T7018 prep for,responsible
R5378 T7020 T7021 det the,recruitment
R5379 T7021 T7019 pobj recruitment,for
R5380 T7022 T7021 prep of,recruitment
R5381 T7023 T7026 amod other,proteins
R5382 T7024 T7026 amod critical,proteins
R5383 T7025 T7026 amod signaling,proteins
R5384 T7026 T7022 pobj proteins,of
R5385 T7027 T7021 prep to,recruitment
R5386 T7028 T7027 pobj CD40,to
R5387 T7029 T7031 punct ",",immunoprecipitated
R5388 T7030 T7031 nsubj we,immunoprecipitated
R5389 T7031 T7031 ROOT immunoprecipitated,immunoprecipitated
R5390 T7032 T7031 dobj CD40,immunoprecipitated
R5391 T7033 T7031 advcl signaling,immunoprecipitated
R5392 T7034 T7033 dobj complexes,signaling
R5393 T7035 T7034 prep from,complexes
R5394 T7036 T7039 amod HOIP-deficient,cells
R5395 T7037 T7036 cc and,HOIP-deficient
R5396 T7038 T7036 conj HOIP-reconstituted,HOIP-deficient
R5397 T7039 T7035 pobj cells,from
R5398 T7040 T7034 punct (,complexes
R5399 T7041 T7042 compound Fig.,5B
R5400 T7042 T7034 appos 5B,complexes
R5401 T7043 T7034 punct ),complexes
R5402 T7044 T7031 punct .,immunoprecipitated
R5403 T7045 T7046 mark As,described
R5404 T7046 T7058 advcl described,contained
R5405 T7047 T7046 advmod previously,described
R5406 T7048 T7058 punct ",",contained
R5407 T7049 T7058 nsubj CD40,contained
R5408 T7050 T7058 advcl signaling,contained
R5409 T7051 T7058 nsubj complexes,contained
R5410 T7052 T7051 acl immunoprecipitated,complexes
R5411 T7053 T7052 prep from,immunoprecipitated
R5412 T7054 T7057 nummod A20,cells
R5413 T7055 T7057 nummod .2,cells
R5414 T7056 T7057 compound J,cells
R5415 T7057 T7053 pobj cells,from
R5416 T7058 T7058 ROOT contained,contained
R5417 T7059 T7058 dobj HOIP,contained
R5418 T7060 T7062 advmod as,as
R5419 T7061 T7062 advmod well,as
R5420 T7062 T7059 cc as,HOIP
R5421 T7063 T7059 conj TRAF2,HOIP
R5422 T7064 T7059 punct ",",HOIP
R5423 T7065 T7059 appos TRAF3,HOIP
R5424 T7066 T7059 punct ",",HOIP
R5425 T7067 T7069 compound IKKα,β
R5426 T7068 T7069 compound /,β
R5427 T7069 T7059 appos β,HOIP
R5428 T7070 T7059 punct ",",HOIP
R5429 T7071 T7059 cc and,HOIP
R5430 T7072 T7073 compound IKKγ,[
R5431 T7073 T7059 conj [,HOIP
R5432 T7074 T7075 nummod 6,]
R5433 T7075 T7059 conj ],HOIP
R5434 T7076 T7058 punct .,contained
R5435 T7077 T7078 det The,amounts
R5436 T7078 T7092 nsubj amounts,were
R5437 T7079 T7078 prep of,amounts
R5438 T7080 T7079 pobj TRAF2,of
R5439 T7081 T7078 punct ",",amounts
R5440 T7082 T7078 appos TRAF3,amounts
R5441 T7083 T7078 punct ",",amounts
R5442 T7084 T7078 cc and,amounts
R5443 T7085 T7078 conj cIAP1,amounts
R5444 T7086 T7085 prep in,cIAP1
R5445 T7087 T7086 pobj CD40,in
R5446 T7088 T7085 dep immunoprecipitates,cIAP1
R5447 T7089 T7088 prep from,immunoprecipitates
R5448 T7090 T7091 amod HOIP-deficient,cells
R5449 T7091 T7089 pobj cells,from
R5450 T7092 T7092 ROOT were,were
R5451 T7093 T7092 acomp similar,were
R5452 T7094 T7093 prep to,similar
R5453 T7095 T7094 pobj those,to
R5454 T7096 T7095 prep from,those
R5455 T7097 T7101 amod parental,cells
R5456 T7098 T7101 compound A20,cells
R5457 T7099 T7100 compound .2,J
R5458 T7100 T7101 compound J,cells
R5459 T7101 T7096 pobj cells,from
R5460 T7102 T7092 punct ",",were
R5461 T7103 T7092 advcl indicating,were
R5462 T7104 T7108 mark that,required
R5463 T7105 T7108 nsubjpass HOIP,required
R5464 T7106 T7108 auxpass is,required
R5465 T7107 T7108 neg not,required
R5466 T7108 T7103 ccomp required,indicating
R5467 T7109 T7108 prep for,required
R5468 T7110 T7111 det the,association
R5469 T7111 T7109 pobj association,for
R5470 T7112 T7111 prep of,association
R5471 T7113 T7114 det these,proteins
R5472 T7114 T7112 pobj proteins,of
R5473 T7115 T7111 prep with,association
R5474 T7116 T7115 pobj CD40,with
R5475 T7117 T7092 punct .,were
R5476 T7118 T7126 prep In,were
R5477 T7119 T7118 pobj contrast,In
R5478 T7120 T7126 punct ",",were
R5479 T7121 T7123 compound IKKα,β
R5480 T7122 T7123 compound /,β
R5481 T7123 T7126 nsubj β,were
R5482 T7124 T7123 cc and,β
R5483 T7125 T7123 conj IKKγ,β
R5484 T7126 T7126 ROOT were,were
R5485 T7127 T7126 neg not,were
R5486 T7128 T7126 acomp detectable,were
R5487 T7129 T7128 prep in,detectable
R5488 T7130 T7131 nummod CD40,immunoprecipitates
R5489 T7131 T7129 pobj immunoprecipitates,in
R5490 T7132 T7131 acl recovered,immunoprecipitates
R5491 T7133 T7132 prep from,recovered
R5492 T7134 T7135 amod HOIP-deficient,cells
R5493 T7135 T7133 pobj cells,from
R5494 T7136 T7132 punct (,recovered
R5495 T7137 T7138 compound Fig.,5B
R5496 T7138 T7138 ROOT 5B,5B
R5497 T7139 T7140 punct ",",C
R5498 T7140 T7138 appos C,5B
R5499 T7141 T7138 punct ),5B
R5500 T7142 T7132 punct .,recovered
R5501 T7143 T7144 det The,amounts
R5502 T7144 T7153 nsubj amounts,immunoprecipitates
R5503 T7145 T7144 prep of,amounts
R5504 T7146 T7148 compound IKKα,β
R5505 T7147 T7148 compound /,β
R5506 T7148 T7145 pobj β,of
R5507 T7149 T7148 cc and,β
R5508 T7150 T7148 conj IKKγ,β
R5509 T7151 T7150 prep in,IKKγ
R5510 T7152 T7151 pobj CD40,in
R5511 T7153 T7157 nsubj immunoprecipitates,were
R5512 T7154 T7153 prep from,immunoprecipitates
R5513 T7155 T7156 amod HOIP-reconstituted,cells
R5514 T7156 T7154 pobj cells,from
R5515 T7157 T7157 ROOT were,were
R5516 T7158 T7157 acomp similar,were
R5517 T7159 T7158 prep to,similar
R5518 T7160 T7159 pobj those,to
R5519 T7161 T7160 acl detected,those
R5520 T7162 T7161 prep in,detected
R5521 T7163 T7162 pobj samples,in
R5522 T7164 T7163 acl prepared,samples
R5523 T7165 T7164 prep from,prepared
R5524 T7166 T7167 amod parental,cells
R5525 T7167 T7165 pobj cells,from
R5526 T7168 T7157 punct ",",were
R5527 T7169 T7157 advcl demonstrating,were
R5528 T7170 T7178 mark that,were
R5529 T7171 T7172 det the,defects
R5530 T7172 T7178 nsubj defects,were
R5531 T7173 T7172 prep in,defects
R5532 T7174 T7175 compound IKK,recruitment
R5533 T7175 T7173 pobj recruitment,in
R5534 T7176 T7177 nsubj we,observed
R5535 T7177 T7172 relcl observed,defects
R5536 T7178 T7169 ccomp were,demonstrating
R5537 T7179 T7178 acomp due,were
R5538 T7180 T7181 advmod specifically,to
R5539 T7181 T7179 prep to,due
R5540 T7182 T7183 det the,absence
R5541 T7183 T7181 pobj absence,to
R5542 T7184 T7183 prep of,absence
R5543 T7185 T7186 compound HOIP,expression
R5544 T7186 T7184 pobj expression,of
R5545 T7187 T7157 punct .,were
R5546 T7188 T7189 det These,data
R5547 T7189 T7190 nsubj data,indicate
R5548 T7190 T7190 ROOT indicate,indicate
R5549 T7191 T7194 mark that,required
R5550 T7192 T7194 nsubjpass HOIP,required
R5551 T7193 T7194 auxpass is,required
R5552 T7194 T7190 ccomp required,indicate
R5553 T7195 T7194 prep for,required
R5554 T7196 T7195 pobj recruitment,for
R5555 T7197 T7196 prep of,recruitment
R5556 T7198 T7200 det the,complex
R5557 T7199 T7200 amod NF-κB-activating,complex
R5558 T7200 T7197 pobj complex,of
R5559 T7201 T7200 prep to,complex
R5560 T7202 T7203 det the,CD40
R5561 T7203 T7204 nsubj CD40,signaling
R5562 T7204 T7201 pcomp signaling,to
R5563 T7205 T7204 dobj complex,signaling
R5564 T7206 T7190 punct .,indicate
R5565 T7207 T7208 mark As,shown
R5566 T7208 T7208 ROOT shown,shown
R5567 T7209 T7208 advmod here,shown
R5568 T7210 T7208 cc and,shown
R5569 T7211 T7220 prep in,present
R5570 T7212 T7214 poss our,study
R5571 T7213 T7214 amod previous,study
R5572 T7214 T7211 pobj study,in
R5573 T7215 T7217 nmod [,]
R5574 T7216 T7217 nummod 6,]
R5575 T7217 T7220 nsubj ],present
R5576 T7218 T7220 punct ",",present
R5577 T7219 T7220 compound IKKγ,present
R5578 T7220 T7224 nsubj present,appears
R5579 T7221 T7220 prep in,present
R5580 T7222 T7221 pobj CD40,in
R5581 T7223 T7224 nsubj immunoprecipitates,appears
R5582 T7224 T7208 conj appears,shown
R5583 T7225 T7226 aux to,have
R5584 T7226 T7224 xcomp have,appears
R5585 T7227 T7230 det a,weight
R5586 T7228 T7230 amod higher,weight
R5587 T7229 T7230 amod molecular,weight
R5588 T7230 T7226 dobj weight,have
R5589 T7231 T7230 prep than,weight
R5590 T7232 T7231 pobj that,than
R5591 T7233 T7232 acl found,that
R5592 T7234 T7233 prep in,found
R5593 T7235 T7236 compound cell,lysates
R5594 T7236 T7234 pobj lysates,in
R5595 T7237 T7224 punct .,appears
R5596 T7238 T7239 aux To,confirm
R5597 T7239 T7251 advcl confirm,generated
R5598 T7240 T7246 mark that,was
R5599 T7241 T7245 det this,species
R5600 T7242 T7244 amod higher,weight
R5601 T7243 T7244 amod molecular,weight
R5602 T7244 T7245 compound weight,species
R5603 T7245 T7246 nsubj species,was
R5604 T7246 T7239 ccomp was,confirm
R5605 T7247 T7246 advmod indeed,was
R5606 T7248 T7246 attr IKKγ,was
R5607 T7249 T7251 punct ",",generated
R5608 T7250 T7251 nsubj we,generated
R5609 T7251 T7251 ROOT generated,generated
R5610 T7252 T7256 compound A20,lines
R5611 T7253 T7254 compound .2,J
R5612 T7254 T7256 compound J,lines
R5613 T7255 T7256 compound cell,lines
R5614 T7256 T7251 dobj lines,generated
R5615 T7257 T7256 acl containing,lines
R5616 T7258 T7262 det a,vector
R5617 T7259 T7260 advmod stably,integrated
R5618 T7260 T7262 amod integrated,vector
R5619 T7261 T7262 amod retroviral,vector
R5620 T7262 T7257 dobj vector,containing
R5621 T7263 T7264 nsubj that,encoded
R5622 T7264 T7262 relcl encoded,vector
R5623 T7265 T7267 det an,version
R5624 T7266 T7267 amod epitope-tagged,version
R5625 T7267 T7264 dobj version,encoded
R5626 T7268 T7267 prep of,version
R5627 T7269 T7268 pobj mouse,of
R5628 T7270 T7267 appos IKKγ,version
R5629 T7271 T7251 punct .,generated
R5630 T7272 T7273 compound Cell,lysates
R5631 T7273 T7282 nsubjpass lysates,analyzed
R5632 T7274 T7273 cc and,lysates
R5633 T7275 T7276 amod CD40,immunoprecipitates
R5634 T7276 T7273 conj immunoprecipitates,lysates
R5635 T7277 T7273 acl prepared,lysates
R5636 T7278 T7277 prep from,prepared
R5637 T7279 T7280 det these,cells
R5638 T7280 T7278 pobj cells,from
R5639 T7281 T7282 auxpass were,analyzed
R5640 T7282 T7282 ROOT analyzed,analyzed
R5641 T7283 T7282 agent by,analyzed
R5642 T7284 T7285 amod Western,blotting
R5643 T7285 T7283 pobj blotting,by
R5644 T7286 T7282 prep for,analyzed
R5645 T7287 T7289 det the,tag
R5646 T7288 T7289 compound epitope,tag
R5647 T7289 T7286 pobj tag,for
R5648 T7290 T7289 punct (,tag
R5649 T7291 T7292 compound Fig.,5C
R5650 T7292 T7289 appos 5C,tag
R5651 T7293 T7289 punct ),tag
R5652 T7294 T7282 punct .,analyzed
R5653 T7295 T7296 det This,analysis
R5654 T7296 T7297 nsubj analysis,produced
R5655 T7297 T7297 ROOT produced,produced
R5656 T7298 T7299 det a,pattern
R5657 T7299 T7297 dobj pattern,produced
R5658 T7300 T7299 prep of,pattern
R5659 T7301 T7300 pobj bands,of
R5660 T7302 T7303 nsubj that,was
R5661 T7303 T7299 relcl was,pattern
R5662 T7304 T7303 advmod essentially,was
R5663 T7305 T7306 det the,same
R5664 T7306 T7303 attr same,was
R5665 T7307 T7306 prep as,same
R5666 T7308 T7309 det that,obtained
R5667 T7309 T7307 pobj obtained,as
R5668 T7310 T7303 advcl using,was
R5669 T7311 T7313 det an,specific
R5670 T7312 T7313 compound antibody,specific
R5671 T7313 T7310 dobj specific,using
R5672 T7314 T7313 prep for,specific
R5673 T7315 T7316 amod native,IKKγ
R5674 T7316 T7314 pobj IKKγ,for
R5675 T7317 T7318 punct (,compare
R5676 T7318 T7310 xcomp compare,using
R5677 T7319 T7318 dobj panels,compare
R5678 T7320 T7318 conj B,compare
R5679 T7321 T7320 cc and,B
R5680 T7322 T7320 conj C,B
R5681 T7323 T7318 prep in,compare
R5682 T7324 T7323 pobj Fig.,in
R5683 T7325 T7324 nummod 5,Fig.
R5684 T7326 T7318 punct ),compare
R5685 T7327 T7297 punct .,produced
R5686 T7328 T7336 advmod Moreover,confirmed
R5687 T7329 T7336 punct ",",confirmed
R5688 T7330 T7336 nsubj analysis,confirmed
R5689 T7331 T7330 prep of,analysis
R5690 T7332 T7331 pobj cells,of
R5691 T7333 T7332 acl expressing,cells
R5692 T7334 T7335 compound epitope,tagged-IKKγ
R5693 T7335 T7333 dobj tagged-IKKγ,expressing
R5694 T7336 T7336 ROOT confirmed,confirmed
R5695 T7337 T7343 mark that,requires
R5696 T7338 T7343 nsubj recruitment,requires
R5697 T7339 T7338 prep of,recruitment
R5698 T7340 T7339 pobj IKKγ,of
R5699 T7341 T7338 prep to,recruitment
R57 T135 T137 compound HOIL-1L,Protein
R5700 T7342 T7341 pobj CD40,to
R5701 T7343 T7336 ccomp requires,confirmed
R5702 T7344 T7343 dobj HOIP,requires
R5703 T7345 T7336 punct .,confirmed
R5704 T7346 T7347 aux To,determine
R5705 T7347 T7366 advcl determine,incubated
R5706 T7348 T7349 nsubj what,was
R5707 T7349 T7347 ccomp was,determine
R5708 T7350 T7349 acomp responsible,was
R5709 T7351 T7350 prep for,responsible
R5710 T7352 T7355 det the,weight
R5711 T7353 T7355 amod increased,weight
R5712 T7354 T7355 amod molecular,weight
R5713 T7355 T7351 pobj weight,for
R5714 T7356 T7355 prep of,weight
R5715 T7357 T7356 pobj IKKγ,of
R5716 T7358 T7355 prep in,weight
R5717 T7359 T7360 compound CD40,immunoprecipitates
R5718 T7360 T7358 pobj immunoprecipitates,in
R5719 T7361 T7366 punct ",",incubated
R5720 T7362 T7364 det the,samples
R5721 T7363 T7364 compound protein,samples
R5722 T7364 T7366 nsubjpass samples,incubated
R5723 T7365 T7366 auxpass were,incubated
R5724 T7366 T7366 ROOT incubated,incubated
R5725 T7367 T7366 prep with,incubated
R5726 T7368 T7369 compound lambda,phosphatase
R5727 T7369 T7367 pobj phosphatase,with
R5728 T7370 T7369 punct ",",phosphatase
R5729 T7371 T7372 nsubj which,removes
R5730 T7372 T7369 relcl removes,phosphatase
R5731 T7373 T7372 dobj phosphates,removes
R5732 T7374 T7373 acl attached,phosphates
R5733 T7375 T7376 aux to,tyrosine
R5734 T7376 T7374 xcomp tyrosine,attached
R5735 T7377 T7376 punct ",",tyrosine
R5736 T7378 T7376 conj threonine,tyrosine
R5737 T7379 T7378 punct ",",threonine
R5738 T7380 T7378 cc or,threonine
R5739 T7381 T7378 conj serine,threonine
R5740 T7382 T7378 conj residues,threonine
R5741 T7383 T7382 prep in,residues
R5742 T7384 T7383 pobj proteins,in
R5743 T7385 T7366 punct .,incubated
R5744 T7386 T7387 det This,treatment
R5745 T7387 T7388 nsubj treatment,reduced
R5746 T7388 T7388 ROOT reduced,reduced
R5747 T7389 T7388 dobj much,reduced
R5748 T7390 T7389 prep of,much
R5749 T7391 T7392 compound CD40-associated,IKKγ
R5750 T7392 T7390 pobj IKKγ,of
R5751 T7393 T7388 prep to,reduced
R5752 T7394 T7397 det an,weight
R5753 T7395 T7397 amod apparent,weight
R5754 T7396 T7397 amod molecular,weight
R5755 T7397 T7393 pobj weight,to
R5756 T7398 T7397 amod similar,weight
R5757 T7399 T7398 prep to,similar
R5758 T7400 T7399 pobj that,to
R5759 T7401 T7400 prep of,that
R5760 T7402 T7403 det the,protein
R5761 T7403 T7401 pobj protein,of
R5762 T7404 T7398 prep in,similar
R5763 T7405 T7406 compound cell,lysates
R5764 T7406 T7404 pobj lysates,in
R5765 T7407 T7409 punct (,5C
R5766 T7408 T7409 compound Fig.,5C
R5767 T7409 T7398 appos 5C,similar
R5768 T7410 T7409 punct ),5C
R5769 T7411 T7388 punct .,reduced
R5770 T7412 T7423 advmod However,remained
R5771 T7413 T7423 punct ",",remained
R5772 T7414 T7415 advmod at,least
R5773 T7415 T7416 advmod least,one
R5774 T7416 T7417 nummod one,band
R5775 T7417 T7423 nsubj band,remained
R5776 T7418 T7417 prep of,band
R5777 T7419 T7420 advmod significantly,higher
R5778 T7420 T7422 amod higher,weight
R5779 T7421 T7422 amod molecular,weight
R5780 T7422 T7418 pobj weight,of
R5781 T7423 T7423 ROOT remained,remained
R5782 T7424 T7423 prep after,remained
R5783 T7425 T7426 compound phosphatase,treatment
R5784 T7426 T7424 pobj treatment,after
R5785 T7427 T7423 punct ",",remained
R5786 T7428 T7423 advcl suggesting,remained
R5787 T7429 T7432 mark that,is
R5788 T7430 T7431 compound CD40-associated,IKKγ
R5789 T7431 T7432 nsubj IKKγ,is
R5790 T7432 T7428 ccomp is,suggesting
R5791 T7433 T7432 acomp subject,is
R5792 T7434 T7433 prep to,subject
R5793 T7435 T7436 advmod at,least
R5794 T7436 T7437 advmod least,one
R5795 T7437 T7439 nummod one,modification
R5796 T7438 T7439 amod other,modification
R5797 T7439 T7434 pobj modification,to
R5798 T7440 T7439 prep in,modification
R5799 T7441 T7440 pobj addition,in
R58 T136 T137 compound Interacting,Protein
R5800 T7442 T7441 prep to,addition
R5801 T7443 T7442 pobj phosphorylation,to
R5802 T7444 T7423 punct .,remained
R5803 T7445 T7449 advmod Overall,demonstrate
R5804 T7446 T7449 punct ",",demonstrate
R5805 T7447 T7448 det these,data
R5806 T7448 T7449 nsubj data,demonstrate
R5807 T7449 T7449 ROOT demonstrate,demonstrate
R5808 T7450 T7453 mark that,required
R5809 T7451 T7453 nsubjpass HOIP,required
R5810 T7452 T7453 auxpass is,required
R5811 T7453 T7449 ccomp required,demonstrate
R5812 T7454 T7453 prep for,required
R5813 T7455 T7456 det the,association
R5814 T7456 T7454 pobj association,for
R5815 T7457 T7456 prep of,association
R5816 T7458 T7457 pobj IKKγ,of
R5817 T7459 T7456 prep with,association
R5818 T7460 T7459 pobj CD40,with
R5819 T7461 T7453 punct ",",required
R5820 T7462 T7453 cc and,required
R5821 T7463 T7449 conj suggest,demonstrate
R5822 T7464 T7468 mark that,coupled
R5823 T7465 T7466 det this,event
R5824 T7466 T7468 nsubjpass event,coupled
R5825 T7467 T7468 auxpass is,coupled
R5826 T7468 T7463 ccomp coupled,suggest
R5827 T7469 T7468 prep to,coupled
R5828 T7470 T7471 amod post-translational,modifications
R5829 T7471 T7469 pobj modifications,to
R5830 T7472 T7471 prep of,modifications
R5831 T7473 T7472 pobj IKKγ,of
R5832 T7474 T7449 punct .,demonstrate
R59 T137 T141 nsubj Protein,Is
R60 T138 T139 punct (,HOIP
R61 T139 T137 appos HOIP,Protein
R62 T140 T137 punct ),Protein
R63 T141 T141 ROOT Is,Is
R64 T142 T141 acomp Essential,Is
R65 T143 T141 prep for,Is
R66 T144 T146 compound CD40,CD40
R67 T145 T146 compound Signaling,CD40
R68 T146 T143 pobj CD40,for
R69 T147 T141 conj is,Is
R70 T148 T151 det a,receptor
R7034 T9222 T9223 poss Our,results
R7035 T9223 T9224 nsubj results,indicate
R7036 T9224 T9224 ROOT indicate,indicate
R7037 T9225 T9229 mark that,is
R7038 T9226 T9227 det the,protein
R7039 T9227 T9229 nsubj protein,is
R7040 T9228 T9229 nsubj HOIP,is
R7041 T9229 T9224 ccomp is,indicate
R7042 T9230 T9229 acomp critical,is
R7043 T9231 T9230 prep for,critical
R7044 T9232 T9233 amod CD40-induced,signals
R7045 T9233 T9231 pobj signals,for
R7046 T9234 T9235 nsubj that,regulate
R7047 T9235 T9233 relcl regulate,signals
R7048 T9236 T9238 compound B,function
R7049 T9237 T9238 compound cell,function
R7050 T9238 T9235 dobj function,regulate
R7051 T9239 T9224 punct .,indicate
R7052 T9240 T9241 poss Our,data
R7053 T9241 T9242 nsubj data,show
R7054 T9242 T9242 ROOT show,show
R7055 T9243 T9247 mark that,include
R7056 T9244 T9246 amod HOIP-dependent,responses
R7057 T9245 T9246 amod cellular,responses
R7058 T9246 T9247 nsubj responses,include
R7059 T9247 T9242 ccomp include,show
R7060 T9248 T9249 amod CD40-mediated,upregulation
R7061 T9249 T9247 dobj upregulation,include
R7062 T9250 T9249 prep of,upregulation
R7063 T9251 T9252 nummod CD80,expression
R7064 T9252 T9250 pobj expression,of
R7065 T9253 T9252 cc and,expression
R7066 T9254 T9252 conj synthesis,expression
R7067 T9255 T9252 prep of,expression
R7068 T9256 T9258 compound germline,transcripts
R7069 T9257 T9258 compound RNA,transcripts
R7070 T9258 T9255 pobj transcripts,of
R7071 T9259 T9258 prep from,transcripts
R7072 T9260 T9264 det the,locus
R7073 T9261 T9264 amod immunoglobulin,locus
R7074 T9262 T9264 amod heavy,locus
R7075 T9263 T9264 compound chain,locus
R7076 T9264 T9259 pobj locus,from
R7077 T9265 T9264 punct ",",locus
R7078 T9266 T9267 nummod two,events
R7079 T9267 T9264 appos events,locus
R7080 T9268 T9269 nsubj that,are
R7081 T9269 T9267 relcl are,events
R7082 T9270 T9269 acomp important,are
R7083 T9271 T9270 prep for,important
R7084 T9272 T9275 amod T-cell-dependent,responses
R7085 T9273 T9275 amod antibody-mediated,responses
R7086 T9274 T9275 amod immune,responses
R7087 T9275 T9271 pobj responses,for
R7088 T9276 T9242 punct .,show
R7089 T9277 T9284 prep At,indicate
R7090 T9278 T9280 det the,level
R7091 T9279 T9280 amod molecular,level
R7092 T9280 T9277 pobj level,At
R7093 T9281 T9284 punct ",",indicate
R7094 T9282 T9283 poss our,data
R7095 T9283 T9284 nsubj data,indicate
R7096 T9284 T9284 ROOT indicate,indicate
R7097 T9285 T9287 mark that,functions
R7098 T9286 T9287 compound HOIP,functions
R7099 T9287 T9284 ccomp functions,indicate
R71 T149 T150 compound cell,surface
R7100 T9288 T9287 dobj downstream,functions
R7101 T9289 T9288 prep of,downstream
R7102 T9290 T9289 pobj TRAF2,of
R7103 T9291 T9288 prep in,downstream
R7104 T9292 T9295 det the,pathway
R7105 T9293 T9295 nmod CD40,pathway
R7106 T9294 T9295 amod signaling,pathway
R7107 T9295 T9291 pobj pathway,in
R7108 T9296 T9287 cc and,functions
R7109 T9297 T9299 mark that,has
R7110 T9298 T9299 nsubj HOIP,has
R7111 T9299 T9287 conj has,functions
R7112 T9300 T9302 det a,role
R7113 T9301 T9302 amod key,role
R7114 T9302 T9299 dobj role,has
R7115 T9303 T9302 prep in,role
R7116 T9304 T9303 pcomp promoting,in
R7117 T9305 T9306 det the,recruitment
R7118 T9306 T9304 dobj recruitment,promoting
R7119 T9307 T9306 prep of,recruitment
R7120 T9308 T9310 det the,complex
R7121 T9309 T9310 compound IKK,complex
R7122 T9310 T9307 pobj complex,of
R7123 T9311 T9310 prep to,complex
R7124 T9312 T9311 pobj CD40,to
R7125 T9313 T9284 punct .,indicate
R7126 T9314 T9319 advmod Consistent,activation
R7127 T9315 T9314 prep with,Consistent
R7128 T9316 T9315 pobj this,with
R7129 T9317 T9319 punct ",",activation
R7130 T9318 T9319 amod CD40-induced,activation
R7131 T9319 T9322 nsubj activation,is
R7132 T9320 T9319 prep of,activation
R7133 T9321 T9320 pobj NF-κB,of
R7134 T9322 T9322 ROOT is,is
R7135 T9323 T9322 acomp dependent,is
R7136 T9324 T9323 prep on,dependent
R7137 T9325 T9326 det the,presence
R7138 T9326 T9324 pobj presence,on
R7139 T9327 T9326 prep of,presence
R7140 T9328 T9327 pobj HOIP,of
R7141 T9329 T9322 punct .,is
R7142 T9330 T9335 prep In,show
R7143 T9331 T9330 pobj addition,In
R7144 T9332 T9335 punct ",",show
R7145 T9333 T9334 poss our,data
R7146 T9334 T9335 nsubj data,show
R7147 T9335 T9335 ROOT show,show
R7148 T9336 T9338 mark that,facilitates
R7149 T9337 T9338 nsubj HOIP,facilitates
R7150 T9338 T9335 ccomp facilitates,show
R7151 T9339 T9340 det the,activation
R7152 T9340 T9338 dobj activation,facilitates
R7153 T9341 T9340 prep of,activation
R7154 T9342 T9341 pobj JNK,of
R7155 T9343 T9338 prep in,facilitates
R7156 T9344 T9343 pobj response,in
R7157 T9345 T9344 prep to,response
R7158 T9346 T9347 amod CD40,engagement
R7159 T9347 T9345 pobj engagement,to
R7160 T9348 T9335 punct .,show
R7161 T9349 T9353 advmod Together,provide
R7162 T9350 T9353 punct ",",provide
R7163 T9351 T9352 poss our,findings
R7164 T9352 T9353 nsubj findings,provide
R7165 T9353 T9353 ROOT provide,provide
R7166 T9354 T9353 dobj support,provide
R7167 T9355 T9354 prep for,support
R7168 T9356 T9357 det the,conclusion
R7169 T9357 T9355 pobj conclusion,for
R7170 T9358 T9360 mark that,is
R7171 T9359 T9360 nsubj HOIP,is
R7172 T9360 T9357 acl is,conclusion
R7173 T9361 T9363 det a,component
R7174 T9362 T9363 amod key,component
R7175 T9363 T9360 attr component,is
R7176 T9364 T9363 prep of,component
R7177 T9365 T9368 det the,pathway
R7178 T9366 T9368 nmod CD40,pathway
R7179 T9367 T9368 amod signaling,pathway
R7180 T9368 T9364 pobj pathway,of
R7181 T9369 T9353 punct .,provide
R7182 T9370 T9386 prep Given,indicate
R7183 T9371 T9372 det the,importance
R7184 T9372 T9370 pobj importance,Given
R7185 T9373 T9372 prep of,importance
R7186 T9374 T9373 pobj CD40,of
R7187 T9375 T9370 pcomp signaling,Given
R7188 T9376 T9375 prep in,signaling
R7189 T9377 T9378 preconj both,cellular
R7190 T9378 T9382 amod cellular,responses
R7191 T9379 T9378 cc and,cellular
R7192 T9380 T9378 conj humoral,cellular
R7193 T9381 T9382 amod immune,responses
R7194 T9382 T9376 pobj responses,in
R7195 T9383 T9386 punct ",",indicate
R7196 T9384 T9385 poss our,results
R7197 T9385 T9386 nsubj results,indicate
R7198 T9386 T9386 ROOT indicate,indicate
R7199 T9387 T9389 mark that,has
R72 T150 T151 compound surface,receptor
R7200 T9388 T9389 nsubj HOIP,has
R7201 T9389 T9386 ccomp has,indicate
R7202 T9390 T9392 det a,role
R7203 T9391 T9392 amod critical,role
R7204 T9392 T9389 dobj role,has
R7205 T9393 T9392 prep in,role
R7206 T9394 T9395 det the,regulation
R7207 T9395 T9393 pobj regulation,in
R7208 T9396 T9395 prep of,regulation
R7209 T9397 T9399 det the,system
R7210 T9398 T9399 amod immune,system
R7211 T9399 T9396 pobj system,of
R7212 T9400 T9386 punct .,indicate
R7213 T9401 T9403 det The,properties
R7214 T9402 T9403 amod functional,properties
R7215 T9403 T9410 nsubjpass properties,characterized
R7216 T9404 T9403 prep of,properties
R7217 T9405 T9404 pobj HOIP,of
R7218 T9406 T9410 aux have,characterized
R7219 T9407 T9410 advmod only,characterized
R7220 T9408 T9410 auxpass been,characterized
R7221 T9409 T9410 advmod partially,characterized
R7222 T9410 T9410 ROOT characterized,characterized
R7223 T9411 T9410 punct .,characterized
R7224 T9412 T9413 amod Initial,studies
R7225 T9413 T9414 nsubj studies,showed
R7226 T9414 T9414 ROOT showed,showed
R7227 T9415 T9422 mark that,are
R7228 T9416 T9422 nsubj HOIP,are
R7229 T9417 T9416 cc and,HOIP
R7230 T9418 T9420 det the,protein
R7231 T9419 T9420 amod related,protein
R7232 T9420 T9416 conj protein,HOIP
R7233 T9421 T9416 conj HOIL-1,HOIP
R7234 T9422 T9414 ccomp are,showed
R7235 T9423 T9422 attr components,are
R7236 T9424 T9423 prep of,components
R7237 T9425 T9430 det a,kDa
R7238 T9426 T9430 amod large,kDa
R7239 T9427 T9430 punct (,kDa
R7240 T9428 T9430 nmod ∼,kDa
R7241 T9429 T9430 nummod 600,kDa
R7242 T9430 T9424 pobj kDa,of
R7243 T9431 T9430 punct ),kDa
R7244 T9432 T9433 npadvmod protein,complex
R7245 T9433 T9434 amod complex,capable
R7246 T9434 T9423 amod capable,components
R7247 T9435 T9434 prep of,capable
R7248 T9436 T9435 pcomp synthesizing,of
R7249 T9437 T9439 amod linear,chains
R7250 T9438 T9439 compound polyubiquitin,chains
R7251 T9439 T9436 dobj chains,synthesizing
R7252 T9440 T9422 conj [,are
R7253 T9441 T9442 nummod 5,]
R7254 T9442 T9440 dobj ],[
R7255 T9443 T9414 punct .,showed
R7256 T9444 T9445 amod Subsequent,studies
R7257 T9445 T9446 nsubj studies,showed
R7258 T9446 T9446 ROOT showed,showed
R7259 T9447 T9452 mark that,interact
R7260 T9448 T9450 det a,complex
R7261 T9449 T9450 compound HOIP-containing,complex
R7262 T9450 T9452 nsubj complex,interact
R7263 T9451 T9452 aux can,interact
R7264 T9452 T9446 ccomp interact,showed
R7265 T9453 T9452 prep with,interact
R7266 T9454 T9453 pobj IKKγ,with
R7267 T9455 T9452 cc and,interact
R7268 T9456 T9452 conj facilitate,interact
R7269 T9457 T9456 dobj activation,facilitate
R7270 T9458 T9457 prep of,activation
R7271 T9459 T9458 pobj NF-κB,of
R7272 T9460 T9456 prep via,facilitate
R7273 T9461 T9463 det the,pathway
R7274 T9462 T9463 amod canonical,pathway
R7275 T9463 T9460 pobj pathway,via
R7276 T9464 T9466 nmod [,]
R7277 T9465 T9466 nummod 16,]
R7278 T9466 T9463 appos ],pathway
R7279 T9467 T9446 punct .,showed
R7280 T9468 T9469 det These,data
R7281 T9469 T9476 nsubj data,suggest
R7282 T9470 T9469 punct ",",data
R7283 T9471 T9469 acl considered,data
R7284 T9472 T9473 advmod together,with
R7285 T9473 T9471 prep with,considered
R7286 T9474 T9473 pobj ours,with
R7287 T9475 T9476 punct ",",suggest
R7288 T9476 T9476 ROOT suggest,suggest
R7289 T9477 T9476 dobj that,suggest
R7290 T9478 T9482 det a,recruitment
R7291 T9479 T9482 amod HOIP-containing,recruitment
R7292 T9480 T9481 amod complex,mediates
R7293 T9481 T9482 compound mediates,recruitment
R7294 T9482 T9477 dobj recruitment,that
R7295 T9483 T9482 prep of,recruitment
R7296 T9484 T9483 pobj IKKγ,of
R7297 T9485 T9482 prep to,recruitment
R7298 T9486 T9487 det the,CD40
R7299 T9487 T9485 pobj CD40,to
R73 T151 T147 attr receptor,is
R7300 T9488 T9482 acl signaling,recruitment
R7301 T9489 T9488 dobj complex,signaling
R7302 T9490 T9476 punct .,suggest
R7303 T9491 T9497 prep In,play
R7304 T9492 T9491 pobj addition,In
R7305 T9493 T9497 punct ",",play
R7306 T9494 T9495 compound CD40-associated,HOIP
R7307 T9495 T9497 nsubj HOIP,play
R7308 T9496 T9497 aux could,play
R7309 T9497 T9497 ROOT play,play
R7310 T9498 T9499 det a,role
R7311 T9499 T9497 dobj role,play
R7312 T9500 T9499 prep in,role
R7313 T9501 T9500 pcomp activating,in
R7314 T9502 T9501 dobj IKKγ,activating
R7315 T9503 T9501 prep after,activating
R7316 T9504 T9505 poss its,recruitment
R7317 T9505 T9503 pobj recruitment,after
R7318 T9506 T9497 prep to,play
R7319 T9507 T9508 det the,signaling
R7320 T9508 T9506 pobj signaling,to
R7321 T9509 T9512 amod complex,]
R7322 T9510 T9512 nmod [,]
R7323 T9511 T9510 nummod 16,[
R7324 T9512 T9508 dobj ],signaling
R7325 T9513 T9497 punct .,play
R7326 T9514 T9518 det The,forms
R7327 T9515 T9517 amod higher,weight
R7328 T9516 T9517 amod molecular,weight
R7329 T9517 T9518 compound weight,forms
R7330 T9518 T9527 nsubj forms,be
R7331 T9519 T9518 prep of,forms
R7332 T9520 T9519 pobj IKKγ,of
R7333 T9521 T9522 nsubj we,observed
R7334 T9522 T9518 relcl observed,forms
R7335 T9523 T9522 prep in,observed
R7336 T9524 T9525 nummod CD40,immunoprecipitates
R7337 T9525 T9523 pobj immunoprecipitates,in
R7338 T9526 T9527 aux would,be
R7339 T9527 T9527 ROOT be,be
R7340 T9528 T9527 acomp consistent,be
R7341 T9529 T9528 prep with,consistent
R7342 T9530 T9531 det the,presence
R7343 T9531 T9529 pobj presence,with
R7344 T9532 T9531 prep of,presence
R7345 T9533 T9534 amod post-transcriptional,modifications
R7346 T9534 T9532 pobj modifications,of
R7347 T9535 T9534 prep including,modifications
R7348 T9536 T9535 pobj phosphorylation,including
R7349 T9537 T9536 cc and,phosphorylation
R7350 T9538 T9536 conj ubiquitination,phosphorylation
R7351 T9539 T9536 punct ",",phosphorylation
R7352 T9540 T9543 nsubjpass which,suggested
R7353 T9541 T9543 aux have,suggested
R7354 T9542 T9543 auxpass been,suggested
R7355 T9543 T9534 relcl suggested,modifications
R7356 T9544 T9545 aux to,reduce
R7357 T9545 T9543 xcomp reduce,suggested
R7358 T9546 T9545 cc or,reduce
R7359 T9547 T9545 conj enhance,reduce
R7360 T9548 T9549 amod IKKγ,activity
R7361 T9549 T9547 dobj activity,enhance
R7362 T9550 T9547 punct ",",enhance
R7363 T9551 T9554 advmod respectively,]
R7364 T9552 T9554 nmod [,]
R7365 T9553 T9554 nummod 16,]
R7366 T9554 T9547 npadvmod ],enhance
R7367 T9555 T9554 punct ",",]
R7368 T9556 T9558 nmod [,]
R7369 T9557 T9558 nummod 17,]
R7370 T9558 T9554 appos ],]
R7371 T9559 T9527 punct .,be
R7372 T9560 T9561 det The,mechanisms
R7373 T9561 T9581 nsubj mechanisms,remain
R7374 T9562 T9565 prep by,mediates
R7375 T9563 T9562 pobj which,by
R7376 T9564 T9565 nsubj HOIP,mediates
R7377 T9565 T9561 relcl mediates,mechanisms
R7378 T9566 T9565 dobj recruitment,mediates
R7379 T9567 T9566 prep of,recruitment
R7380 T9568 T9570 det the,complex
R7381 T9569 T9570 compound IKK,complex
R7382 T9570 T9567 pobj complex,of
R7383 T9571 T9570 prep to,complex
R7384 T9572 T9571 pobj CD40,to
R7385 T9573 T9565 cc and,mediates
R7386 T9574 T9578 prep by,recruited
R7387 T9575 T9574 pobj which,by
R7388 T9576 T9578 nsubjpass HOIP,recruited
R7389 T9577 T9578 auxpass is,recruited
R7390 T9578 T9565 conj recruited,mediates
R7391 T9579 T9578 prep to,recruited
R7392 T9580 T9581 nsubj CD40,remain
R7393 T9581 T9581 ROOT remain,remain
R7394 T9582 T9581 acomp unclear,remain
R7395 T9583 T9581 punct .,remain
R7396 T9584 T9586 det A,study
R7397 T9585 T9586 amod previous,study
R7398 T9586 T9587 nsubj study,indicates
R7399 T9587 T9587 ROOT indicates,indicates
R74 T152 T151 amod important,receptor
R7400 T9588 T9591 mark that,mediate
R7401 T9589 T9591 nsubj HOIP,mediate
R7402 T9590 T9591 aux may,mediate
R7403 T9591 T9587 ccomp mediate,indicates
R7404 T9592 T9593 amod direct,contacts
R7405 T9593 T9591 dobj contacts,mediate
R7406 T9594 T9593 prep with,contacts
R7407 T9595 T9600 det the,]
R7408 T9596 T9598 nmod IKK,[
R7409 T9597 T9598 amod complex,[
R7410 T9598 T9600 nmod [,]
R7411 T9599 T9600 nummod 16,]
R7412 T9600 T9594 pobj ],with
R7413 T9601 T9587 punct ",",indicates
R7414 T9602 T9587 advcl suggesting,indicates
R7415 T9603 T9605 mark that,functions
R7416 T9604 T9605 nsubj it,functions
R7417 T9605 T9602 ccomp functions,suggesting
R7418 T9606 T9605 prep as,functions
R7419 T9607 T9608 det an,adaptor
R7420 T9608 T9606 pobj adaptor,as
R7421 T9609 T9608 prep for,adaptor
R7422 T9610 T9611 det the,recruitment
R7423 T9611 T9609 pobj recruitment,for
R7424 T9612 T9611 prep of,recruitment
R7425 T9613 T9615 det the,complex
R7426 T9614 T9615 compound IKK,complex
R7427 T9615 T9612 pobj complex,of
R7428 T9616 T9615 prep to,complex
R7429 T9617 T9616 pobj CD40,to
R7430 T9618 T9587 punct .,indicates
R7431 T9619 T9627 advmod However,suggests
R7432 T9620 T9627 punct ",",suggests
R7433 T9621 T9624 det the,activity
R7434 T9622 T9624 compound ubiquitin,activity
R7435 T9623 T9624 compound ligase,activity
R7436 T9624 T9627 nsubj activity,suggests
R7437 T9625 T9624 prep of,activity
R7438 T9626 T9625 pobj HOIP,of
R7439 T9627 T9627 ROOT suggests,suggests
R7440 T9628 T9630 mark that,is
R7441 T9629 T9630 nsubj it,is
R7442 T9630 T9627 ccomp is,suggests
R7443 T9631 T9630 acomp more,is
R7444 T9632 T9631 prep than,more
R7445 T9633 T9636 det a,molecule
R7446 T9634 T9636 amod simple,molecule
R7447 T9635 T9636 compound adapter,molecule
R7448 T9636 T9632 pobj molecule,than
R7449 T9637 T9627 punct .,suggests
R7450 T9638 T9640 mark As,appears
R7451 T9639 T9640 nsubj IKKγ,appears
R7452 T9640 T9655 advcl appears,is
R7453 T9641 T9640 oprd capable,appears
R7454 T9642 T9641 prep of,capable
R7455 T9643 T9646 amod binding,[
R7456 T9644 T9645 compound linear,polyubiquitin
R7457 T9645 T9646 compound polyubiquitin,[
R7458 T9646 T9642 pobj [,of
R7459 T9647 T9648 nummod 18,]
R7460 T9648 T9652 nmod ],]
R7461 T9649 T9648 punct ",",]
R7462 T9650 T9648 conj [,]
R7463 T9651 T9652 nummod 19,]
R7464 T9652 T9640 dobj ],appears
R7465 T9653 T9655 punct ",",is
R7466 T9654 T9655 nsubj it,is
R7467 T9655 T9655 ROOT is,is
R7468 T9656 T9655 acomp possible,is
R7469 T9657 T9659 mark that,directs
R7470 T9658 T9659 nsubj HOIP,directs
R7471 T9659 T9655 advcl directs,is
R7472 T9660 T9659 dobj formation,directs
R7473 T9661 T9660 prep of,formation
R7474 T9662 T9664 amod linear,chains
R7475 T9663 T9664 compound polyubiquitin,chains
R7476 T9664 T9661 pobj chains,of
R7477 T9665 T9659 prep on,directs
R7478 T9666 T9668 det a,factor
R7479 T9667 T9668 amod CD40-associated,factor
R7480 T9668 T9665 pobj factor,on
R7481 T9669 T9659 punct ",",directs
R7482 T9670 T9659 cc and,directs
R7483 T9671 T9672 nsubj it,is
R7484 T9672 T9659 conj is,directs
R7485 T9673 T9674 det these,chains
R7486 T9674 T9672 attr chains,is
R7487 T9675 T9676 nsubj that,serve
R7488 T9676 T9674 relcl serve,chains
R7489 T9677 T9678 aux to,recruit
R7490 T9678 T9676 xcomp recruit,serve
R7491 T9679 T9678 dobj IKKγ,recruit
R7492 T9680 T9678 prep to,recruit
R7493 T9681 T9682 det the,CD40
R7494 T9682 T9683 nsubj CD40,signaling
R7495 T9683 T9680 pcomp signaling,to
R7496 T9684 T9683 dobj complex,signaling
R7497 T9685 T9672 punct .,is
R7498 T9686 T9688 det The,interactions
R7499 T9687 T9688 amod molecular,interactions
R75 T153 T152 prep in,important
R7500 T9688 T9701 nsubj interactions,remain
R7501 T9689 T9688 amod necessary,interactions
R7502 T9690 T9689 prep for,necessary
R7503 T9691 T9690 pobj recruitment,for
R7504 T9692 T9691 prep of,recruitment
R7505 T9693 T9692 pobj HOIP,of
R7506 T9694 T9693 appos itself,HOIP
R7507 T9695 T9691 prep to,recruitment
R7508 T9696 T9697 det the,CD40
R7509 T9697 T9699 compound CD40,complex
R7510 T9698 T9699 compound signaling,complex
R7511 T9699 T9695 pobj complex,to
R7512 T9700 T9701 advmod also,remain
R7513 T9701 T9701 ROOT remain,remain
R7514 T9702 T9705 aux to,characterized
R7515 T9703 T9705 auxpass be,characterized
R7516 T9704 T9705 advmod fully,characterized
R7517 T9705 T9701 xcomp characterized,remain
R7518 T9706 T9701 punct .,remain
R7519 T9707 T9709 nsubj We,showed
R7520 T9708 T9709 advmod previously,showed
R7521 T9709 T9709 ROOT showed,showed
R7522 T9710 T9709 dobj that,showed
R7523 T9711 T9712 det the,recruitment
R7524 T9712 T9710 dobj recruitment,that
R7525 T9713 T9712 prep of,recruitment
R7526 T9714 T9713 pobj HOIP,of
R7527 T9715 T9712 prep to,recruitment
R7528 T9716 T9717 det the,signaling
R7529 T9717 T9715 pobj signaling,to
R7530 T9718 T9719 nsubj complex,is
R7531 T9719 T9717 pcomp is,signaling
R7532 T9720 T9721 amod TRAF2-dependent,[
R7533 T9721 T9719 attr [,is
R7534 T9722 T9723 nummod 8,]
R7535 T9723 T9721 appos ],[
R7536 T9724 T9709 punct .,showed
R7537 T9725 T9731 advmod Potentially,interact
R7538 T9726 T9731 punct ",",interact
R7539 T9727 T9731 nsubj TRAF2,interact
R7540 T9728 T9727 cc and,TRAF2
R7541 T9729 T9731 nsubj HOIP,interact
R7542 T9730 T9731 advmod directly,interact
R7543 T9731 T9731 ROOT interact,interact
R7544 T9732 T9731 punct ",",interact
R7545 T9733 T9731 cc but,interact
R7546 T9734 T9735 nsubj it,is
R7547 T9735 T9731 conj is,interact
R7548 T9736 T9735 acomp possible,is
R7549 T9737 T9760 mark that,generates
R7550 T9738 T9741 det the,activity
R7551 T9739 T9741 nmod ubiquitin,activity
R7552 T9740 T9741 compound ligase,activity
R7553 T9741 T9760 nsubj activity,generates
R7554 T9742 T9741 prep of,activity
R7555 T9743 T9746 nummod TRAF2,]
R7556 T9744 T9746 nmod [,]
R7557 T9745 T9746 nummod 20,]
R7558 T9746 T9742 pobj ],of
R7559 T9747 T9746 punct (,]
R7560 T9748 T9746 cc or,]
R7561 T9749 T9750 amod TRAF2-associated,proteins
R7562 T9750 T9746 conj proteins,]
R7563 T9751 T9750 punct ",",proteins
R7564 T9752 T9753 amod such,as
R7565 T9753 T9750 prep as,proteins
R7566 T9754 T9760 det the,generates
R7567 T9755 T9756 compound cIAPs,[
R7568 T9756 T9760 nsubj [,generates
R7569 T9757 T9758 nummod 21,]
R7570 T9758 T9756 appos ],[
R7571 T9759 T9756 punct ),[
R7572 T9760 T9735 ccomp generates,is
R7573 T9761 T9763 amod K63-linked,chains
R7574 T9762 T9763 compound polyubiquitin,chains
R7575 T9763 T9760 dobj chains,generates
R7576 T9764 T9768 prep to,bind
R7577 T9765 T9764 pobj which,to
R7578 T9766 T9768 nsubj HOIP,bind
R7579 T9767 T9768 aux can,bind
R7580 T9768 T9760 advcl bind,generates
R7581 T9769 T9768 cc and,bind
R7582 T9770 T9771 advmod thus,associate
R7583 T9771 T9768 conj associate,bind
R7584 T9772 T9771 prep with,associate
R7585 T9773 T9774 det the,CD40
R7586 T9774 T9775 nsubj CD40,signaling
R7587 T9775 T9772 pcomp signaling,with
R7588 T9776 T9779 amod complex,]
R7589 T9777 T9779 nmod [,]
R7590 T9778 T9777 nummod 22,[
R7591 T9779 T9775 dobj ],signaling
R7592 T9780 T9735 punct .,is
R7593 T9781 T9785 mark While,indicated
R7594 T9782 T9784 poss our,work
R7595 T9783 T9784 amod previous,work
R7596 T9784 T9785 nsubj work,indicated
R7597 T9785 T9804 advcl indicated,are
R7598 T9786 T9789 det a,link
R7599 T9787 T9788 advmod potentially,important
R76 T154 T155 det the,activation
R7600 T9788 T9789 amod important,link
R7601 T9789 T9785 dobj link,indicated
R7602 T9790 T9789 prep between,link
R7603 T9791 T9790 pobj TRAF2,between
R7604 T9792 T9791 cc and,TRAF2
R7605 T9793 T9791 conj HOIP,TRAF2
R7606 T9794 T9793 prep in,HOIP
R7607 T9795 T9794 pobj CD40,in
R7608 T9796 T9793 advcl signaling,HOIP
R7609 T9797 T9796 punct ",",signaling
R7610 T9798 T9799 det the,signals
R7611 T9799 T9796 dobj signals,signaling
R7612 T9800 T9799 cc and,signals
R7613 T9801 T9799 conj functions,signals
R7614 T9802 T9799 acl tested,signals
R7615 T9803 T9802 advmod here,tested
R7616 T9804 T9804 ROOT are,are
R7617 T9805 T9804 acomp dependent,are
R7618 T9806 T9804 prep upon,are
R7619 T9807 T9806 pobj TRAF6,upon
R7620 T9808 T9810 advmod as,as
R7621 T9809 T9810 advmod well,as
R7622 T9810 T9807 cc as,TRAF6
R7623 T9811 T9807 conj TRAF2,TRAF6
R7624 T9812 T9804 punct .,are
R7625 T9813 T9824 prep In,found
R7626 T9814 T9815 amod previous,experiments
R7627 T9815 T9813 pobj experiments,In
R7628 T9816 T9815 prep with,experiments
R7629 T9817 T9821 amod TRAF-deficient,cells
R7630 T9818 T9821 compound A20,cells
R7631 T9819 T9820 compound .2,J
R7632 T9820 T9821 compound J,cells
R7633 T9821 T9816 pobj cells,with
R7634 T9822 T9824 punct ",",found
R7635 T9823 T9824 nsubj we,found
R7636 T9824 T9824 ROOT found,found
R7637 T9825 T9834 mark that,mediated
R7638 T9826 T9827 det the,activation
R7639 T9827 T9834 nsubjpass activation,mediated
R7640 T9828 T9827 prep of,activation
R7641 T9829 T9828 pobj NF-κB,of
R7642 T9830 T9827 prep by,activation
R7643 T9831 T9830 pobj CD40,by
R7644 T9832 T9834 aux could,mediated
R7645 T9833 T9834 auxpass be,mediated
R7646 T9834 T9824 ccomp mediated,found
R7647 T9835 T9834 agent by,mediated
R7648 T9836 T9837 det either,TRAF2
R7649 T9837 T9835 pobj TRAF2,by
R7650 T9838 T9837 cc or,TRAF2
R7651 T9839 T9837 conj TRAF6,TRAF2
R7652 T9840 T9834 punct ",",mediated
R7653 T9841 T9847 mark while,was
R7654 T9842 T9847 nsubj activation,was
R7655 T9843 T9842 prep of,activation
R7656 T9844 T9843 pobj JNK,of
R7657 T9845 T9842 prep by,activation
R7658 T9846 T9845 pobj CD40,by
R7659 T9847 T9834 advcl was,mediated
R7660 T9848 T9849 advmod largely,dependent
R7661 T9849 T9847 acomp dependent,was
R7662 T9850 T9849 prep on,dependent
R7663 T9851 T9855 nummod TRAF6,]
R7664 T9852 T9851 advmod alone,TRAF6
R7665 T9853 T9855 amod [,]
R7666 T9854 T9855 nummod 8,]
R7667 T9855 T9850 pobj ],on
R7668 T9856 T9824 punct .,found
R7669 T9857 T9859 nsubj HOIP,compromises
R7670 T9858 T9859 nsubj deficiency,compromises
R7671 T9859 T9859 ROOT compromises,compromises
R7672 T9860 T9862 det the,activation
R7673 T9861 T9862 amod CD40-mediated,activation
R7674 T9862 T9859 dobj activation,compromises
R7675 T9863 T9862 prep of,activation
R7676 T9864 T9865 preconj both,NF-κB
R7677 T9865 T9863 pobj NF-κB,of
R7678 T9866 T9865 cc and,NF-κB
R7679 T9867 T9865 conj JNK,NF-κB
R7680 T9868 T9859 punct ",",compromises
R7681 T9869 T9859 advcl indicating,compromises
R7682 T9870 T9879 mark that,pass
R7683 T9871 T9879 nsubj signals,pass
R7684 T9872 T9871 acl mediated,signals
R7685 T9873 T9872 agent by,mediated
R7686 T9874 T9875 det both,TRAF2
R7687 T9875 T9873 pobj TRAF2,by
R7688 T9876 T9875 cc and,TRAF2
R7689 T9877 T9875 conj TRAF6,TRAF2
R7690 T9878 T9879 amod likely,pass
R7691 T9879 T9869 ccomp pass,indicating
R7692 T9880 T9879 prep through,pass
R7693 T9881 T9880 pobj HOIP,through
R7694 T9882 T9859 punct .,compromises
R7695 T9883 T9885 poss Our,work
R7696 T9884 T9885 amod previous,work
R7697 T9885 T9887 nsubj work,demonstrated
R7698 T9886 T9887 advmod also,demonstrated
R7699 T9887 T9887 ROOT demonstrated,demonstrated
R77 T155 T153 pobj activation,in
R7700 T9888 T9902 mark that,compromised
R7701 T9889 T9891 det the,activation
R7702 T9890 T9891 amod CD40-mediated,activation
R7703 T9891 T9902 nsubjpass activation,compromised
R7704 T9892 T9891 prep of,activation
R7705 T9893 T9892 pobj NF-κB,of
R7706 T9894 T9893 cc and,NF-κB
R7707 T9895 T9893 conj JNK,NF-κB
R7708 T9896 T9891 punct ",",activation
R7709 T9897 T9902 mark while,compromised
R7710 T9898 T9902 nsubjpass TRAF6-dependent,compromised
R7711 T9899 T9902 punct ",",compromised
R7712 T9900 T9902 auxpass was,compromised
R7713 T9901 T9902 neg not,compromised
R7714 T9902 T9887 ccomp compromised,demonstrated
R7715 T9903 T9902 agent by,compromised
R7716 T9904 T9905 det the,disruption
R7717 T9905 T9903 pobj disruption,by
R7718 T9906 T9905 prep of,disruption
R7719 T9907 T9909 det the,site
R7720 T9908 T9909 amod binding,site
R7721 T9909 T9906 pobj site,of
R7722 T9910 T9902 prep for,compromised
R7723 T9911 T9910 pobj TRAF6,for
R7724 T9912 T9902 prep in,compromised
R7725 T9913 T9915 det the,domain
R7726 T9914 T9915 amod cytoplasmic,domain
R7727 T9915 T9912 pobj domain,in
R7728 T9916 T9915 prep of,domain
R7729 T9917 T9916 pobj CD40,of
R7730 T9918 T9917 cc or,CD40
R7731 T9919 T9915 conj deletion,domain
R7732 T9920 T9915 prep of,domain
R7733 T9921 T9924 det the,domain
R7734 T9922 T9924 amod receptor,domain
R7735 T9923 T9924 amod binding,domain
R7736 T9924 T9920 pobj domain,of
R7737 T9925 T9924 punct (,domain
R7738 T9926 T9928 det the,domain
R7739 T9927 T9928 compound TRAF-C,domain
R7740 T9928 T9924 appos domain,domain
R7741 T9929 T9924 punct ),domain
R7742 T9930 T9902 prep in,compromised
R7743 T9931 T9933 quantmod TRAF6,8
R7744 T9932 T9933 quantmod [,8
R7745 T9933 T9930 pobj 8,in
R7746 T9934 T9902 npadvmod ],compromised
R7747 T9935 T9887 punct .,demonstrated
R7748 T9936 T9937 det These,observations
R7749 T9937 T9938 nsubj observations,indicate
R7750 T9938 T9938 ROOT indicate,indicate
R7751 T9939 T9944 mark that,bind
R7752 T9940 T9944 nsubj TRAF6,bind
R7753 T9941 T9944 aux need,bind
R7754 T9942 T9944 neg not,bind
R7755 T9943 T9944 advmod directly,bind
R7756 T9944 T9938 ccomp bind,indicate
R7757 T9945 T9944 dobj CD40,bind
R7758 T9946 T9944 prep in,bind
R7759 T9947 T9946 pobj order,in
R7760 T9948 T9949 aux to,mediate
R7761 T9949 T9947 acl mediate,order
R7762 T9950 T9951 amod certain,signals
R7763 T9951 T9949 dobj signals,mediate
R7764 T9952 T9944 punct ",",bind
R7765 T9953 T9938 advcl suggesting,indicate
R7766 T9954 T9955 det the,assembly
R7767 T9955 T9953 dobj assembly,suggesting
R7768 T9956 T9955 prep of,assembly
R7769 T9957 T9959 det a,complex
R7770 T9958 T9959 compound signaling,complex
R7771 T9959 T9956 pobj complex,of
R7772 T9960 T9962 neg not,associated
R7773 T9961 T9962 advmod directly,associated
R7774 T9962 T9959 acl associated,complex
R7775 T9963 T9962 prep with,associated
R7776 T9964 T9965 det the,receptor
R7777 T9965 T9963 pobj receptor,with
R7778 T9966 T9938 punct .,indicate
R7779 T9967 T9971 mark If,exists
R7780 T9968 T9971 predet such,exists
R7781 T9969 T9971 det a,exists
R7782 T9970 T9971 amod complex,exists
R7783 T9971 T9975 advcl exists,indicate
R7784 T9972 T9975 punct ",",indicate
R7785 T9973 T9974 poss our,results
R7786 T9974 T9975 nsubj results,indicate
R7787 T9975 T9975 ROOT indicate,indicate
R7788 T9976 T9981 mark that,compromises
R7789 T9977 T9978 det the,absence
R7790 T9978 T9981 nsubj absence,compromises
R7791 T9979 T9978 prep of,absence
R7792 T9980 T9979 pobj HOIP,of
R7793 T9981 T9975 ccomp compromises,indicate
R7794 T9982 T9983 poss its,function
R7795 T9983 T9981 dobj function,compromises
R7796 T9984 T9985 advmod as,well
R7797 T9985 T9981 advmod well,compromises
R7798 T9986 T9975 punct .,indicate
R7799 T9987 T9990 mark Although,presented
R78 T156 T155 prep of,activation
R7800 T9988 T9989 det the,experiments
R7801 T9989 T9990 nsubj experiments,presented
R7802 T9990 T9992 advcl presented,focus
R7803 T9991 T9990 advmod here,presented
R7804 T9992 T9992 ROOT focus,focus
R7805 T9993 T9992 prep on,focus
R7806 T9994 T9993 pobj CD40,on
R7807 T9995 T9994 punct ",",CD40
R7808 T9996 T9997 poss our,results
R7809 T9997 T9994 conj results,CD40
R7810 T9998 T9992 cc and,focus
R7811 T9999 T9992 conj those,focus
R7812 T10000 T9999 prep of,those
R7813 T10001 T10002 amod other,groups
R7814 T10002 T10000 pobj groups,of
R7815 T10003 T10005 nmod [,]
R7816 T10004 T10005 nummod 16,]
R7817 T10005 T9999 conj ],those
R7818 T10006 T10005 punct ",",]
R7819 T10007 T10005 conj [,]
R7820 T10008 T10007 nummod 22,[
R7821 T10009 T10010 amod ],support
R7822 T10010 T10010 ROOT support,support
R7823 T10011 T10012 det the,possibility
R7824 T10012 T10010 dobj possibility,support
R7825 T10013 T10015 mark that,is
R7826 T10014 T10015 nsubj HOIP,is
R7827 T10015 T10012 acl is,possibility
R7828 T10016 T10015 acomp important,is
R7829 T10017 T10015 prep in,is
R7830 T10018 T10020 amod many,pathways
R7831 T10019 T10020 amod signaling,pathways
R7832 T10020 T10017 pobj pathways,in
R7833 T10021 T10027 prep in,involved
R7834 T10022 T10021 pobj which,in
R7835 T10023 T10027 nsubjpass TRAF2,involved
R7836 T10024 T10023 cc or,TRAF2
R7837 T10025 T10023 conj TRAF6,TRAF2
R7838 T10026 T10027 auxpass are,involved
R7839 T10027 T10020 relcl involved,pathways
R7840 T10028 T10027 punct ",",involved
R7841 T10029 T10020 prep including,pathways
R7842 T10030 T10029 pobj those,including
R7843 T10031 T10030 acl associated,those
R7844 T10032 T10031 prep with,associated
R7845 T10033 T10034 amod various,members
R7846 T10034 T10032 pobj members,with
R7847 T10035 T10034 prep of,members
R7848 T10036 T10038 det the,receptor
R7849 T10037 T10038 compound TNF,receptor
R7850 T10038 T10035 pobj receptor,of
R7851 T10039 T10029 pobj superfamily,including
R7852 T10040 T10039 cc and,superfamily
R7853 T10041 T10043 det the,receptors
R7854 T10042 T10043 amod Toll-like,receptors
R7855 T10043 T10039 conj receptors,superfamily
R7856 T10044 T10010 punct .,support
R7857 T10045 T10047 det The,importance
R7858 T10046 T10047 amod potential,importance
R7859 T10047 T10047 ROOT importance,importance
R7860 T10048 T10047 prep of,importance
R7861 T10049 T10048 pobj HOIP,of
R7862 T10050 T10047 prep in,importance
R7863 T10051 T10052 amod immune,function
R7864 T10052 T10050 pobj function,in
R7865 T10053 T10052 cc and,function
R7866 T10054 T10055 compound TNFR,family
R7867 T10055 T10052 conj family,function
R7868 T10056 T10047 acl signaling,importance
R7869 T10057 T10059 auxpass is,supported
R7870 T10058 T10059 advmod further,supported
R7871 T10059 T10056 ccomp supported,signaling
R7872 T10060 T10059 agent by,supported
R7873 T10061 T10063 det the,discovery
R7874 T10062 T10063 amod recent,discovery
R7875 T10063 T10060 pobj discovery,by
R7876 T10064 T10066 dobj that,interacts
R7877 T10065 T10066 nsubj HOIP,interacts
R7878 T10066 T10063 relcl interacts,discovery
R7879 T10067 T10066 prep with,interacts
R7880 T10068 T10069 det a,protein
R7881 T10069 T10067 pobj protein,with
R7882 T10070 T10069 acl known,protein
R7883 T10071 T10070 prep as,known
R7884 T10072 T10071 pobj SHARPIN,as
R7885 T10073 T10072 punct (,SHARPIN
R7886 T10074 T10076 amod SHANK-associated,domain
R7887 T10075 T10076 compound RH,domain
R7888 T10076 T10072 appos domain,SHARPIN
R7889 T10077 T10078 amod interacting,protein
R7890 T10078 T10072 appos protein,SHARPIN
R7891 T10079 T10078 prep in,protein
R7892 T10080 T10081 amod postsynaptic,density
R7893 T10081 T10079 pobj density,in
R7894 T10082 T10072 punct ),SHARPIN
R7895 T10083 T10072 punct ",",SHARPIN
R7896 T10084 T10085 nsubj which,appears
R7897 T10085 T10072 relcl appears,SHARPIN
R7898 T10086 T10085 oprd capable,appears
R7899 T10087 T10086 prep of,capable
R79 T157 T158 amod antigen-presenting,cells
R7900 T10088 T10087 pcomp working,of
R7901 T10089 T10090 advmod together,with
R7902 T10090 T10088 prep with,working
R7903 T10091 T10090 pobj HOIP,with
R7904 T10092 T10091 cc and,HOIP
R7905 T10093 T10091 conj HOIL,HOIP
R7906 T10094 T10095 aux to,mediate
R7907 T10095 T10088 advcl mediate,working
R7908 T10096 T10097 det the,assembly
R7909 T10097 T10095 dobj assembly,mediate
R7910 T10098 T10097 prep of,assembly
R7911 T10099 T10100 amod linear,polyubiquitin
R7912 T10100 T10098 pobj polyubiquitin,of
R7913 T10101 T10103 nmod [,]
R7914 T10102 T10103 nummod 14,]
R7915 T10103 T10097 appos ],assembly
R7916 T10104 T10103 punct ",",]
R7917 T10105 T10103 npadvmod [,]
R7918 T10106 T10107 nummod 23,]
R7919 T10107 T10103 conj ],]
R7920 T10108 T10107 punct ",",]
R7921 T10109 T10111 nmod [,]
R7922 T10110 T10111 nummod 24,]
R7923 T10111 T10107 conj ],]
R7924 T10112 T10047 punct .,importance
R7925 T10113 T10134 advmod Interestingly,exhibit
R7926 T10114 T10134 punct ",",exhibit
R7927 T10115 T10134 nsubj mice,exhibit
R7928 T10116 T10115 prep with,mice
R7929 T10117 T10119 det a,mutation
R7930 T10118 T10119 amod spontaneous,mutation
R7931 T10119 T10116 pobj mutation,with
R7932 T10120 T10119 prep in,mutation
R7933 T10121 T10122 det the,gene
R7934 T10122 T10120 pobj gene,in
R7935 T10123 T10134 advcl encoding,exhibit
R7936 T10124 T10123 dobj SHARPIN,encoding
R7937 T10125 T10128 punct (,dermatitis
R7938 T10126 T10128 amod chronic,dermatitis
R7939 T10127 T10128 amod proliferative,dermatitis
R7940 T10128 T10124 appos dermatitis,SHARPIN
R7941 T10129 T10128 punct (,dermatitis
R7942 T10130 T10128 appos cpdm,dermatitis
R7943 T10131 T10128 punct ),dermatitis
R7944 T10132 T10128 appos mice,dermatitis
R7945 T10133 T10128 punct ),dermatitis
R7946 T10134 T10134 ROOT exhibit,exhibit
R7947 T10135 T10136 amod chronic,inflammation
R7948 T10136 T10134 dobj inflammation,exhibit
R7949 T10137 T10136 prep of,inflammation
R7950 T10138 T10139 det the,skin
R7951 T10139 T10145 nmod skin,development
R7952 T10140 T10139 cc and,skin
R7953 T10141 T10142 amod internal,organs
R7954 T10142 T10139 conj organs,skin
R7955 T10143 T10142 punct ",",organs
R7956 T10144 T10145 amod defective,development
R7957 T10145 T10137 pobj development,of
R7958 T10146 T10145 prep of,development
R7959 T10147 T10149 amod secondary,tissue
R7960 T10148 T10149 amod lymphoid,tissue
R7961 T10149 T10146 pobj tissue,of
R7962 T10150 T10134 punct ",",exhibit
R7963 T10151 T10134 cc and,exhibit
R7964 T10152 T10134 conj defects,exhibit
R7965 T10153 T10152 prep in,defects
R7966 T10154 T10155 det the,production
R7967 T10155 T10153 pobj production,in
R7968 T10156 T10155 prep of,production
R7969 T10157 T10159 amod switched,isotypes
R7970 T10158 T10159 compound immunoglobulin,isotypes
R7971 T10159 T10160 compound isotypes,[
R7972 T10160 T10162 nmod [,]
R7973 T10161 T10162 nummod 17,]
R7974 T10162 T10156 pobj ],of
R7975 T10163 T10162 punct ",",]
R7976 T10164 T10166 nmod [,]
R7977 T10165 T10166 nummod 25,]
R7978 T10166 T10162 appos ],]
R7979 T10167 T10134 punct .,exhibit
R7980 T10168 T10172 det The,link
R7981 T10169 T10170 advmod apparently,intimate
R7982 T10170 T10172 amod intimate,link
R7983 T10171 T10172 amod functional,link
R7984 T10172 T10178 nsubj link,suggests
R7985 T10173 T10172 prep between,link
R7986 T10174 T10173 pobj SHARPIN,between
R7987 T10175 T10174 cc and,SHARPIN
R7988 T10176 T10174 conj HOIP,SHARPIN
R7989 T10177 T10178 advmod strongly,suggests
R7990 T10178 T10178 ROOT suggests,suggests
R7991 T10179 T10187 mark that,stems
R7992 T10180 T10181 advmod at,least
R7993 T10181 T10182 advmod least,part
R7994 T10182 T10187 nsubj part,stems
R7995 T10183 T10182 prep of,part
R7996 T10184 T10186 det the,phenotype
R7997 T10185 T10186 compound cpdm,phenotype
R7998 T10186 T10183 pobj phenotype,of
R7999 T10187 T10178 ccomp stems,suggests
R80 T158 T156 pobj cells,of
R8000 T10188 T10187 prep from,stems
R8001 T10189 T10188 pobj defects,from
R8002 T10190 T10189 prep in,defects
R8003 T10191 T10192 det the,regulation
R8004 T10192 T10190 pobj regulation,in
R8005 T10193 T10192 cc or,regulation
R8006 T10194 T10192 conj function,regulation
R8007 T10195 T10192 prep of,regulation
R8008 T10196 T10195 pobj HOIP,of
R8009 T10197 T10178 punct .,suggests
R81 T159 T152 prep during,important
R817 T1199 T1200 nummod CD40,signaling
R818 T1200 T1216 nsubj signaling,is
R819 T1201 T1200 prep in,signaling
R82 T160 T161 amod immune,responses
R820 T1202 T1204 amod professional,cells
R821 T1203 T1204 amod antigen-presenting,cells
R822 T1204 T1201 pobj cells,in
R823 T1205 T1204 punct ",",cells
R824 T1206 T1204 prep including,cells
R825 T1207 T1208 compound B,cells
R826 T1208 T1206 pobj cells,including
R827 T1209 T1208 punct ",",cells
R828 T1210 T1208 conj macrophages,cells
R829 T1211 T1210 punct ",",macrophages
R83 T161 T159 pobj responses,during
R830 T1212 T1210 cc and,macrophages
R831 T1213 T1214 amod dendritic,cells
R832 T1214 T1210 conj cells,macrophages
R833 T1215 T1216 punct ",",is
R834 T1216 T1216 ROOT is,is
R835 T1217 T1216 acomp critical,is
R836 T1218 T1217 prep for,critical
R837 T1219 T1221 det the,activation
R838 T1220 T1221 amod efficient,activation
R839 T1221 T1218 pobj activation,for
R84 T162 T141 punct .,Is
R840 T1222 T1221 prep of,activation
R841 T1223 T1227 amod humoral,responses
R842 T1224 T1223 cc and,humoral
R843 T1225 T1223 conj cell-mediated,humoral
R844 T1226 T1227 amod immune,responses
R845 T1227 T1222 pobj responses,of
R846 T1228 T1230 nmod [,]
R847 T1229 T1230 nummod 1,]
R848 T1230 T1216 npadvmod ],is
R849 T1231 T1230 punct ",",]
R85 T163 T176 prep In,provides
R850 T1232 T1234 nmod [,]
R851 T1233 T1234 nummod 2,]
R852 T1234 T1230 conj ],]
R853 T1235 T1234 punct ",",]
R854 T1236 T1234 conj [,]
R855 T1237 T1238 nummod 3,]
R856 T1238 T1234 conj ],]
R857 T1239 T1216 punct .,is
R858 T1240 T1243 nsubjpass CD40,activated
R859 T1241 T1243 nsubjpass signaling,activated
R86 T164 T163 pobj macrophages,In
R860 T1242 T1243 auxpass is,activated
R861 T1243 T1243 ROOT activated,activated
R862 T1244 T1243 prep in,activated
R863 T1245 T1248 det a,manner
R864 T1246 T1248 compound T,manner
R865 T1247 T1248 compound cell-dependent,manner
R866 T1248 T1244 pobj manner,in
R867 T1249 T1243 punct ",",activated
R868 T1250 T1259 prep as,expressed
R869 T1251 T1252 det the,ligand
R87 T165 T164 cc and,macrophages
R870 T1252 T1250 pobj ligand,as
R871 T1253 T1252 prep for,ligand
R872 T1254 T1253 pobj CD40,for
R873 T1255 T1254 punct ",",CD40
R874 T1256 T1254 appos CD154,CD40
R875 T1257 T1259 punct ",",expressed
R876 T1258 T1259 auxpass is,expressed
R877 T1259 T1243 advcl expressed,activated
R878 T1260 T1261 advmod primarily,by
R879 T1261 T1259 agent by,expressed
R88 T166 T167 amod dendritic,cells
R880 T1262 T1264 amod activated,cells
R881 T1263 T1264 compound T,cells
R882 T1264 T1261 pobj cells,by
R883 T1265 T1243 punct .,activated
R884 T1266 T1267 nummod CD40,engagement
R885 T1267 T1268 nsubj engagement,leads
R886 T1268 T1268 ROOT leads,leads
R887 T1269 T1268 prep to,leads
R888 T1270 T1271 det the,activation
R889 T1271 T1269 pobj activation,to
R89 T167 T164 conj cells,macrophages
R890 T1272 T1271 prep of,activation
R891 T1273 T1275 amod various,molecules
R892 T1274 T1275 amod signaling,molecules
R893 T1275 T1272 pobj molecules,of
R894 T1276 T1275 punct ",",molecules
R895 T1277 T1275 prep including,molecules
R896 T1278 T1280 amod stress-activated,kinases
R897 T1279 T1280 compound protein,kinases
R898 T1280 T1277 pobj kinases,including
R899 T1281 T1280 cc and,kinases
R90 T168 T167 punct ",",cells
R900 T1282 T1284 det the,factor
R901 T1283 T1284 compound transcription,factor
R902 T1284 T1280 conj factor,kinases
R903 T1285 T1284 appos NF-κB,factor
R904 T1286 T1284 punct ",",factor
R905 T1287 T1288 nsubj which,upregulate
R906 T1288 T1284 relcl upregulate,factor
R907 T1289 T1290 det the,expression
R908 T1290 T1288 dobj expression,upregulate
R909 T1291 T1290 prep of,expression
R91 T169 T176 nsubj engagement,provides
R910 T1292 T1291 pobj cytokines,of
R911 T1293 T1292 cc and,cytokines
R912 T1294 T1295 amod other,factors
R913 T1295 T1292 conj factors,cytokines
R914 T1296 T1297 nsubj that,promote
R915 T1297 T1292 relcl promote,cytokines
R916 T1298 T1299 amod immune,responses
R917 T1299 T1297 dobj responses,promote
R918 T1300 T1268 punct .,leads
R919 T1301 T1302 det The,mechanism
R92 T170 T169 prep of,engagement
R920 T1302 T1314 nsubjpass mechanism,defined
R921 T1303 T1306 prep by,induces
R922 T1304 T1303 pobj which,by
R923 T1305 T1306 nsubj CD40,induces
R924 T1306 T1302 relcl induces,mechanism
R9246 T11924 T11925 compound Cell,lines
R9247 T11925 T11937 nsubjpass lines,described
R9248 T11926 T11930 det The,line
R9249 T11927 T11929 compound mouse,cell
R925 T1307 T1309 det these,pathways
R9250 T11928 T11929 compound B,cell
R9251 T11929 T11930 compound cell,line
R9252 T11930 T11937 nsubjpass line,described
R9253 T11931 T11933 compound A20,J
R9254 T11932 T11933 compound .2,J
R9255 T11933 T11930 appos J,line
R9256 T11934 T11937 aux has,described
R9257 T11935 T11937 auxpass been,described
R9258 T11936 T11937 advmod previously,described
R9259 T11937 T11937 ROOT described,described
R926 T1308 T1309 amod signaling,pathways
R9260 T11938 T11940 nmod [,]
R9261 T11939 T11940 nummod 26,]
R9262 T11940 T11937 dobj ],described
R9263 T11941 T11940 punct ",",]
R9264 T11942 T11944 nmod [,]
R9265 T11943 T11944 nummod 27,]
R9266 T11944 T11940 appos ],]
R9267 T11945 T11937 punct .,described
R9268 T11946 T11948 nsubjpass Cells,grown
R9269 T11947 T11948 auxpass were,grown
R927 T1309 T1306 dobj pathways,induces
R9270 T11948 T11948 ROOT grown,grown
R9271 T11949 T11948 prep in,grown
R9272 T11950 T11949 pobj RPMI,in
R9273 T11951 T11950 nummod 1640,RPMI
R9274 T11952 T11950 punct ",",RPMI
R9275 T11953 T11954 nummod 10,%
R9276 T11954 T11955 npadvmod %,FCS
R9277 T11955 T11950 appos FCS,RPMI
R9278 T11956 T11955 punct ",",FCS
R9279 T11957 T11959 nummod 10,2-ME
R928 T1310 T1314 aux has,defined
R9280 T11958 T11959 compound µM,2-ME
R9281 T11959 T11955 appos 2-ME,FCS
R9282 T11960 T11955 punct ",",FCS
R9283 T11961 T11963 nummod 2,L-glutamine
R9284 T11962 T11963 compound mM,L-glutamine
R9285 T11963 T11955 appos L-glutamine,FCS
R9286 T11964 T11963 punct ",",L-glutamine
R9287 T11965 T11963 cc and,L-glutamine
R9288 T11966 T11955 appos antibiotics,FCS
R9289 T11967 T11948 punct .,grown
R929 T1311 T1314 neg not,defined
R9290 T11968 T11970 nummod HI-5,cells
R9291 T11969 T11970 compound insect,cells
R9292 T11970 T11978 nsubjpass cells,described
R9293 T11971 T11972 punct (,Invitrogen
R9294 T11972 T11970 appos Invitrogen,cells
R9295 T11973 T11970 punct ),cells
R9296 T11974 T11970 acl expressing,cells
R9297 T11975 T11974 dobj CD154,expressing
R9298 T11976 T11978 aux have,described
R9299 T11977 T11978 auxpass been,described
R93 T171 T170 pobj CD40,of
R930 T1312 T1314 auxpass been,defined
R9300 T11978 T11978 ROOT described,described
R9301 T11979 T11978 dobj [,described
R9302 T11980 T11981 nummod 28,]
R9303 T11981 T11979 appos ],[
R9304 T11982 T11978 punct .,described
R931 T1313 T1314 advmod completely,defined
R932 T1314 T1314 ROOT defined,defined
R933 T1315 T1314 punct .,defined
R934 T1316 T1318 det The,domain
R935 T1317 T1318 amod cytoplasmic,domain
R936 T1318 T1323 nsubj domain,appear
R9364 T12059 T12103 nsubj Antibodies,were
R9365 T12060 T12063 nmod Mouse,antibody
R9366 T12061 T12063 amod anti-birch,antibody
R9367 T12062 T12063 compound profilin,antibody
R9368 T12063 T12103 nsubj antibody,were
R9369 T12064 T12066 punct (,[
R937 T1319 T1318 prep of,domain
R9370 T12065 T12066 compound 4A6,[
R9371 T12066 T12063 appos [,antibody
R9372 T12067 T12066 nummod 29,[
R9373 T12068 T12066 appos ],[
R9374 T12069 T12066 punct ),[
R9375 T12070 T12066 punct ",",[
R9376 T12071 T12073 compound rat,CD40
R9377 T12072 T12073 compound anti-mouse,CD40
R9378 T12073 T12066 conj CD40,[
R9379 T12074 T12076 punct (,[
R938 T1320 T1319 pobj CD40,of
R9380 T12075 T12076 compound 1C10,[
R9381 T12076 T12078 npadvmod [,]
R9382 T12077 T12076 nummod 30,[
R9383 T12078 T12073 appos ],CD40
R9384 T12079 T12078 punct ),]
R9385 T12080 T12073 cc and,CD40
R9386 T12081 T12085 det a,antibody
R9387 T12082 T12085 nmod rat,antibody
R9388 T12083 T12085 compound isotype,antibody
R9389 T12084 T12085 compound control,antibody
R939 T1321 T1323 aux does,appear
R9390 T12085 T12073 conj antibody,CD40
R9391 T12086 T12087 punct (,mAb72
R9392 T12087 T12085 appos mAb72,antibody
R9393 T12088 T12087 punct ",",mAb72
R9394 T12089 T12092 compound Developmental,Bank
R9395 T12090 T12092 compound Studies,Bank
R9396 T12091 T12092 compound Hybridoma,Bank
R9397 T12092 T12085 conj Bank,antibody
R9398 T12093 T12092 punct ",",Bank
R9399 T12094 T12092 conj University,Bank
R94 T172 T169 prep by,engagement
R940 T1322 T1323 neg not,appear
R9400 T12095 T12094 prep of,University
R9401 T12096 T12095 pobj Iowa,of
R9402 T12097 T12096 punct ",",Iowa
R9403 T12098 T12099 compound Iowa,City
R9404 T12099 T12094 conj City,University
R9405 T12100 T12099 punct ",",City
R9406 T12101 T12099 conj IA,City
R9407 T12102 T12092 punct ),Bank
R9408 T12103 T12103 ROOT were,were
R9409 T12104 T12103 acomp isolated,were
R941 T1323 T1323 ROOT appear,appear
R9410 T12105 T12104 prep from,isolated
R9411 T12106 T12107 compound hybridoma,supernatants
R9412 T12107 T12105 pobj supernatants,from
R9413 T12108 T12103 punct .,were
R9414 T12109 T12111 amod FITC-labeled,CD80
R9415 T12110 T12111 amod anti-mouse,CD80
R9416 T12111 T12117 nsubj CD80,were
R9417 T12112 T12111 cc and,CD80
R9418 T12113 T12115 det an,control
R9419 T12114 T12115 compound isotype,control
R942 T1324 T1325 aux to,have
R9420 T12115 T12111 conj control,CD80
R9421 T12116 T12117 nsubj Ab,were
R9422 T12117 T12117 ROOT were,were
R9423 T12118 T12117 prep from,were
R9424 T12119 T12118 pobj eBiosciences,from
R9425 T12120 T12117 punct .,were
R9426 T12121 T12126 nmod Rabbit,]
R9427 T12122 T12126 nmod anti-HOIP,]
R9428 T12123 T12124 compound Ab,[
R9429 T12124 T12122 appos [,anti-HOIP
R943 T1325 T1323 xcomp have,appear
R9430 T12125 T12126 nummod 6,]
R9431 T12126 T12127 nsubj ],was
R9432 T12127 T12127 ROOT was,was
R9433 T12128 T12130 det the,gift
R9434 T12129 T12130 compound kind,gift
R9435 T12130 T12127 attr gift,was
R9436 T12131 T12130 prep of,gift
R9437 T12132 T12134 compound Dr.,Eipper
R9438 T12133 T12134 compound Betty,Eipper
R9439 T12134 T12131 pobj Eipper,of
R944 T1326 T1328 amod intrinsic,activity
R9440 T12135 T12134 punct (,Eipper
R9441 T12136 T12134 appos University,Eipper
R9442 T12137 T12136 prep of,University
R9443 T12138 T12137 pobj Connecticut,of
R9444 T12139 T12140 compound Health,Center
R9445 T12140 T12134 appos Center,Eipper
R9446 T12141 T12140 punct ",",Center
R9447 T12142 T12134 npadvmod Farmington,Eipper
R9448 T12143 T12142 punct ",",Farmington
R9449 T12144 T12142 appos Connecticut,Farmington
R945 T1327 T1328 amod enzymatic,activity
R9450 T12145 T12142 punct ),Farmington
R9451 T12146 T12127 punct .,was
R9452 T12147 T12149 compound Goat,IgG
R9453 T12148 T12149 compound anti-rat,IgG
R9454 T12149 T12155 nsubj IgG,were
R9455 T12150 T12149 punct ",",IgG
R9456 T12151 T12149 cc and,IgG
R9457 T12152 T12154 amod HRP-labeled,Abs
R9458 T12153 T12154 amod secondary,Abs
R9459 T12154 T12149 conj Abs,IgG
R946 T1328 T1325 dobj activity,have
R9460 T12155 T12155 ROOT were,were
R9461 T12156 T12155 prep from,were
R9462 T12157 T12159 compound Jackson,Laboratories
R9463 T12158 T12159 compound ImmunoResearch,Laboratories
R9464 T12159 T12156 pobj Laboratories,from
R9465 T12160 T12159 punct ",",Laboratories
R9466 T12161 T12159 conj Inc.,Laboratories
R9467 T12162 T12155 punct .,were
R947 T1329 T1323 punct ",",appear
R948 T1330 T1323 cc but,appear
R949 T1331 T1323 conj is,appear
R95 T173 T175 poss its,CD154
R950 T1332 T1331 acomp able,is
R951 T1333 T1334 aux to,mediate
R952 T1334 T1332 xcomp mediate,able
R953 T1335 T1334 dobj signaling,mediate
R954 T1336 T1335 prep through,signaling
R955 T1337 T1338 det the,recruitment
R956 T1338 T1336 pobj recruitment,through
R957 T1339 T1338 prep of,recruitment
R958 T1340 T1342 amod several,proteins
R9585 T12332 T12334 amod Somatic,gene
R9586 T12333 T12334 compound cell,gene
R9587 T12334 T12342 nsubjpass gene,accomplished
R9588 T12335 T12334 acl targeting,gene
R9589 T12336 T12337 det The,generation
R959 T1341 T1342 amod intracellular,proteins
R9590 T12337 T12335 dobj generation,targeting
R9591 T12338 T12337 prep of,generation
R9592 T12339 T12340 amod HOIP-deficient,cells
R9593 T12340 T12338 pobj cells,of
R9594 T12341 T12342 auxpass was,accomplished
R9595 T12342 T12342 ROOT accomplished,accomplished
R9596 T12343 T12342 advcl using,accomplished
R9597 T12344 T12347 det a,approach
R9598 T12345 T12347 amod homologous,approach
R9599 T12346 T12347 compound recombination,approach
R96 T174 T175 compound ligand,CD154
R960 T1342 T1339 pobj proteins,of
R9600 T12347 T12343 dobj approach,using
R9601 T12348 T12342 conj described,accomplished
R9602 T12349 T12350 advmod previously,[
R9603 T12350 T12352 amod [,]
R9604 T12351 T12352 nummod 7,]
R9605 T12352 T12348 dobj ],described
R9606 T12353 T12352 punct ",",]
R9607 T12354 T12356 nmod [,]
R9608 T12355 T12356 nummod 8,]
R9609 T12356 T12352 appos ],]
R961 T1343 T1331 punct .,is
R9610 T12357 T12342 punct .,accomplished
R9611 T12358 T12367 nsubj Segments,construct
R9612 T12359 T12358 prep of,Segments
R9613 T12360 T12362 amod Rnf31,sequence
R9614 T12361 T12362 compound gene,sequence
R9615 T12362 T12359 pobj sequence,of
R9616 T12363 T12362 acl used,sequence
R9617 T12364 T12363 prep in,used
R9618 T12365 T12366 det the,targeting
R9619 T12366 T12364 pobj targeting,in
R962 T1344 T1365 nsubj Members,appear
R9620 T12367 T12367 ROOT construct,construct
R9621 T12368 T12370 punct (,1A
R9622 T12369 T12370 compound Fig.,1A
R9623 T12370 T12367 dobj 1A,construct
R9624 T12371 T12367 punct ),construct
R9625 T12372 T12373 auxpass were,amplified
R9626 T12373 T12367 advcl amplified,construct
R9627 T12374 T12373 agent by,amplified
R9628 T12375 T12374 pobj PCR,by
R9629 T12376 T12373 prep from,amplified
R963 T1345 T1344 prep of,Members
R9630 T12377 T12379 nmod A20,J
R9631 T12378 T12379 nummod .2,J
R9632 T12379 T12381 nmod J,DNA
R9633 T12380 T12381 amod genomic,DNA
R9634 T12381 T12376 pobj DNA,from
R9635 T12382 T12367 punct .,construct
R9636 T12383 T12385 det The,primers
R9637 T12384 T12385 compound oligonucleotide,primers
R9638 T12385 T12397 nsubj primers,were
R9639 T12386 T12385 acl used,primers
R964 T1346 T1349 det the,factor
R9640 T12387 T12388 aux to,generate
R9641 T12388 T12386 xcomp generate,used
R9642 T12389 T12392 det the,flank
R9643 T12390 T12392 nummod 5,flank
R9644 T12391 T12392 compound ′,flank
R9645 T12392 T12388 dobj flank,generate
R9646 T12393 T12392 punct (,flank
R9647 T12394 T12395 nummod 1224,bp
R9648 T12395 T12392 appos bp,flank
R9649 T12396 T12392 punct ),flank
R965 T1347 T1349 nmod TNF,factor
R9650 T12397 T12397 ROOT were,were
R9651 T12398 T12401 nummod 5,ttttctagagcggtggcttaagtgaccc-3
R9652 T12399 T12401 punct ′,ttttctagagcggtggcttaagtgaccc-3
R9653 T12400 T12401 punct -,ttttctagagcggtggcttaagtgaccc-3
R9654 T12401 T12402 compound ttttctagagcggtggcttaagtgaccc-3,′
R9655 T12402 T12397 attr ′,were
R9656 T12403 T12402 cc and,′
R9657 T12404 T12407 nummod 5,tattctagatgcagcatctgagaaagcaagc-3
R9658 T12405 T12407 punct ′,tattctagatgcagcatctgagaaagcaagc-3
R9659 T12406 T12407 punct -,tattctagatgcagcatctgagaaagcaagc-3
R966 T1348 T1349 amod receptor-associated,factor
R9660 T12407 T12408 amod tattctagatgcagcatctgagaaagcaagc-3,′
R9661 T12408 T12402 conj ′,′
R9662 T12409 T12397 punct .,were
R9663 T12410 T12413 det The,flank
R9664 T12411 T12412 nummod 3,′
R9665 T12412 T12413 compound ′,flank
R9666 T12413 T12419 nsubj flank,were
R9667 T12414 T12413 punct (,flank
R9668 T12415 T12413 appos 6032,flank
R9669 T12416 T12413 appos bp,flank
R967 T1349 T1345 pobj factor,of
R9670 T12417 T12413 punct ),flank
R9671 T12418 T12419 nsubj primers,were
R9672 T12419 T12419 ROOT were,were
R9673 T12420 T12424 nummod 5,′
R9674 T12421 T12423 punct ′,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9675 T12422 T12423 punct -,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9676 T12423 T12424 compound aaaaccggtgtatgcttctttacgggagaaaaatattag-3,′
R9677 T12424 T12419 attr ′,were
R9678 T12425 T12424 cc and,′
R9679 T12426 T12429 nummod 5,tataccggtatgaagccaaaggaacactgagag-3
R968 T1350 T1349 punct (,factor
R9680 T12427 T12429 punct ′,tataccggtatgaagccaaaggaacactgagag-3
R9681 T12428 T12429 punct -,tataccggtatgaagccaaaggaacactgagag-3
R9682 T12429 T12430 compound tataccggtatgaagccaaaggaacactgagag-3,′
R9683 T12430 T12424 conj ′,′
R9684 T12431 T12419 punct .,were
R9685 T12432 T12434 compound Restriction,sites
R9686 T12433 T12434 compound endonuclease,sites
R9687 T12434 T12434 ROOT sites,sites
R9688 T12435 T12434 prep in,sites
R9689 T12436 T12438 det the,primers
R969 T1351 T1349 appos TRAF,factor
R9690 T12437 T12438 compound oligonucleotide,primers
R9691 T12438 T12435 pobj primers,in
R9692 T12439 T12440 amod allowed,insertion
R9693 T12440 T12434 conj insertion,sites
R9694 T12441 T12440 prep of,insertion
R9695 T12442 T12444 det the,products
R9696 T12443 T12444 compound PCR,products
R9697 T12444 T12441 pobj products,of
R9698 T12445 T12434 prep into,sites
R9699 T12446 T12448 det the,vector
R97 T175 T172 pobj CD154,by
R970 T1352 T1351 punct ),TRAF
R9700 T12447 T12448 compound targeting,vector
R9701 T12448 T12445 pobj vector,into
R9702 T12449 T12434 punct .,sites
R9703 T12450 T12453 nummod A20,cells
R9704 T12451 T12453 nummod .2,cells
R9705 T12452 T12453 compound J,cells
R9706 T12453 T12455 nsubjpass cells,transfected
R9707 T12454 T12455 auxpass were,transfected
R9708 T12455 T12455 ROOT transfected,transfected
R9709 T12456 T12455 punct (,transfected
R971 T1353 T1349 conj family,factor
R9710 T12457 T12455 agent by,transfected
R9711 T12458 T12459 compound electroporation,[
R9712 T12459 T12457 pobj [,by
R9713 T12460 T12461 nummod 7,]
R9714 T12461 T12459 appos ],[
R9715 T12462 T12461 punct ),]
R9716 T12463 T12455 prep with,transfected
R9717 T12464 T12465 det the,targeting
R9718 T12465 T12463 pobj targeting,with
R9719 T12466 T12455 conj construct,transfected
R972 T1354 T1353 punct ",",family
R9720 T12467 T12466 cc and,construct
R9721 T12468 T12466 conj subcloned,construct
R9722 T12469 T12468 prep in,subcloned
R9723 T12470 T12469 pobj medium,in
R9724 T12471 T12470 acl containing,medium
R9725 T12472 T12476 nummod 600,G418
R9726 T12473 T12476 compound µg,G418
R9727 T12474 T12476 compound /,G418
R9728 T12475 T12476 compound ml,G418
R9729 T12476 T12477 compound G418,sulfate
R973 T1355 T1344 prep including,Members
R9730 T12477 T12471 dobj sulfate,containing
R9731 T12478 T12455 punct .,transfected
R9732 T12479 T12480 amod Homologous,recombination
R9733 T12480 T12485 nsubjpass recombination,detected
R9734 T12481 T12480 prep in,recombination
R9735 T12482 T12483 amod G418-resistant,clones
R9736 T12483 T12481 pobj clones,in
R9737 T12484 T12485 auxpass was,detected
R9738 T12485 T12485 ROOT detected,detected
R9739 T12486 T12485 agent by,detected
R974 T1356 T1355 pobj TRAF1,including
R9740 T12487 T12486 pobj PCR,by
R9741 T12488 T12487 prep of,PCR
R9742 T12489 T12490 amod genomic,DNA
R9743 T12490 T12488 pobj DNA,of
R9744 T12491 T12485 punct ",",detected
R9745 T12492 T12493 mark as,described
R9746 T12493 T12485 advcl described,detected
R9747 T12494 T12496 nmod [,]
R9748 T12495 T12496 nummod 7,]
R9749 T12496 T12493 dobj ],described
R975 T1357 T1356 punct ",",TRAF1
R9750 T12497 T12485 punct .,detected
R9751 T12498 T12499 compound Oligonucleotide,primers
R9752 T12499 T12503 nsubj primers,were
R9753 T12500 T12499 acl used,primers
R9754 T12501 T12500 prep for,used
R9755 T12502 T12501 pobj screening,for
R9756 T12503 T12517 ccomp were,noted
R9757 T12504 T12507 nummod 5,cttcctgatctcagctttaccgtcac-3
R9758 T12505 T12507 punct ′,cttcctgatctcagctttaccgtcac-3
R9759 T12506 T12507 punct -,cttcctgatctcagctttaccgtcac-3
R976 T1358 T1356 appos TRAF2,TRAF1
R9760 T12507 T12508 amod cttcctgatctcagctttaccgtcac-3,′
R9761 T12508 T12503 attr ′,were
R9762 T12509 T12510 punct (,homologous
R9763 T12510 T12517 ccomp homologous,noted
R9764 T12511 T12510 prep to,homologous
R9765 T12512 T12513 amod genomic,sequence
R9766 T12513 T12511 pobj sequence,to
R9767 T12514 T12517 punct ;,noted
R9768 T12515 T12516 amod approximate,position
R9769 T12516 T12517 nsubj position,noted
R977 T1359 T1356 punct ",",TRAF1
R9770 T12517 T12517 ROOT noted,noted
R9771 T12518 T12517 prep in,noted
R9772 T12519 T12518 pobj Fig.,in
R9773 T12520 T12519 nummod 1,Fig.
R9774 T12521 T12517 punct ),noted
R9775 T12522 T12517 cc and,noted
R9776 T12523 T12526 nummod 5,caatccatcttgttcagccat-3
R9777 T12524 T12526 punct ′,caatccatcttgttcagccat-3
R9778 T12525 T12526 punct -,caatccatcttgttcagccat-3
R9779 T12526 T12527 compound caatccatcttgttcagccat-3,′
R978 T1360 T1356 appos TRAF3,TRAF1
R9780 T12527 T12527 ROOT ′,′
R9781 T12528 T12529 punct (,homologous
R9782 T12529 T12527 parataxis homologous,′
R9783 T12530 T12531 aux to,sequence
R9784 T12531 T12529 relcl sequence,homologous
R9785 T12532 T12531 prep in,sequence
R9786 T12533 T12532 pobj NeoR,in
R9787 T12534 T12529 punct ),homologous
R9788 T12535 T12517 punct .,noted
R9789 T12536 T12548 nsubjpass Clones,transfected
R979 T1361 T1344 punct ",",Members
R9790 T12537 T12545 prep in,disrupted
R9791 T12538 T12537 pobj which,in
R9792 T12539 T12540 nummod one,copy
R9793 T12540 T12545 nsubjpass copy,disrupted
R9794 T12541 T12540 prep of,copy
R9795 T12542 T12541 pobj Rnf31,of
R9796 T12543 T12545 aux had,disrupted
R9797 T12544 T12545 auxpass been,disrupted
R9798 T12545 T12536 relcl disrupted,Clones
R9799 T12546 T12548 auxpass were,transfected
R98 T176 T176 ROOT provides,provides
R980 T1362 T1344 cc and,Members
R9800 T12547 T12548 advmod transiently,transfected
R9801 T12548 T12548 ROOT transfected,transfected
R9802 T12549 T12548 prep with,transfected
R9803 T12550 T12552 det an,plasmid
R9804 T12551 T12552 compound expression,plasmid
R9805 T12552 T12549 pobj plasmid,with
R9806 T12553 T12554 compound encoding,Cre
R9807 T12554 T12548 npadvmod Cre,transfected
R9808 T12555 T12548 punct ",",transfected
R9809 T12556 T12548 prep in,transfected
R981 T1363 T1365 nsubj TRAF6,appear
R9810 T12557 T12556 pobj order,in
R9811 T12558 T12559 aux to,remove
R9812 T12559 T12557 acl remove,order
R9813 T12560 T12559 dobj NeoR,remove
R9814 T12561 T12548 punct .,transfected
R9815 T12562 T12563 amod G418-sensitive,subclones
R9816 T12563 T12565 nsubjpass subclones,subjected
R9817 T12564 T12565 auxpass were,subjected
R9818 T12565 T12565 ROOT subjected,subjected
R9819 T12566 T12565 prep to,subjected
R982 T1364 T1365 punct ",",appear
R9820 T12567 T12569 det a,round
R9821 T12568 T12569 amod second,round
R9822 T12569 T12566 pobj round,to
R9823 T12570 T12569 prep of,round
R9824 T12571 T12570 pobj targeting,of
R9825 T12572 T12573 aux to,disrupt
R9826 T12573 T12571 xcomp disrupt,targeting
R9827 T12574 T12576 det the,copy
R9828 T12575 T12576 amod remaining,copy
R9829 T12576 T12573 dobj copy,disrupt
R983 T1365 T1365 ROOT appear,appear
R9830 T12577 T12576 prep of,copy
R9831 T12578 T12577 pobj Rnf31,of
R9832 T12579 T12565 punct .,subjected
R9833 T12580 T12581 amod G418-resistant,clones
R9834 T12581 T12583 nsubjpass clones,tested
R9835 T12582 T12583 auxpass were,tested
R9836 T12583 T12583 ROOT tested,tested
R9837 T12584 T12583 prep for,tested
R9838 T12585 T12586 amod homologous,recombination
R9839 T12586 T12584 pobj recombination,for
R984 T1366 T1367 aux to,be
R9840 T12587 T12586 prep by,recombination
R9841 T12588 T12587 pobj PCR,by
R9842 T12589 T12588 cc and,PCR
R9843 T12590 T12592 compound HOIP,expression
R9844 T12591 T12592 compound protein,expression
R9845 T12592 T12583 dobj expression,tested
R9846 T12593 T12583 agent by,tested
R9847 T12594 T12595 amod Western,blot
R9848 T12595 T12593 pobj blot,by
R9849 T12596 T12583 punct .,tested
R985 T1367 T1365 xcomp be,appear
R986 T1368 T1369 advmod particularly,important
R987 T1369 T1367 acomp important,be
R988 T1370 T1369 prep for,important
R989 T1371 T1372 det the,initiation
R99 T177 T176 dobj signals,provides
R990 T1372 T1370 pobj initiation,for
R991 T1373 T1372 cc and,initiation
R992 T1374 T1372 conj regulation,initiation
R993 T1375 T1372 prep of,initiation
R994 T1376 T1375 pobj CD40,of
R995 T1377 T1367 advcl signaling,be
R996 T1378 T1380 nmod [,]
R997 T1379 T1380 nummod 4,]
R998 T1380 T1377 dobj ],signaling
R999 T1381 T1365 punct .,appear