PMC:2817507 / 8506-9349 22 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T4190 | 0-7 | NNP | denotes | Nuclear |
| T4191 | 8-16 | NNS | denotes | proteins |
| T4192 | 17-21 | VBD | denotes | were |
| T4193 | 22-30 | VBN | denotes | isolated |
| T4194 | 31-35 | IN | denotes | from |
| T4195 | 36-41 | NN | denotes | brain |
| T4196 | 42-48 | NN | denotes | tissue |
| T4197 | 48-49 | . | denotes | . |
| T4198 | 50-57 | RB | denotes | Briefly |
| T4199 | 57-58 | , | denotes | , |
| T4200 | 59-64 | IN | denotes | after |
| T4201 | 65-72 | NN | denotes | removal |
| T4202 | 73-75 | IN | denotes | of |
| T4203 | 76-87 | JJ | denotes | supernatant |
| T4204 | 88-89 | -LRB- | denotes | ( |
| T4205 | 89-100 | JJ | denotes | cytoplasmic |
| T4206 | 101-108 | VB | denotes | extract |
| T4207 | 108-109 | -RRB- | denotes | ) |
| T4208 | 109-110 | , | denotes | , |
| T4209 | 111-114 | DT | denotes | the |
| T4210 | 115-122 | NNS | denotes | pellets |
| T4211 | 123-127 | VBD | denotes | were |
| T4212 | 128-139 | VBN | denotes | resuspended |
| T4213 | 140-142 | IN | denotes | in |
| T4214 | 143-150 | JJ | denotes | nuclear |
| T4215 | 151-161 | NN | denotes | extraction |
| T4216 | 162-169 | NN | denotes | reagent |
| T4217 | 169-170 | . | denotes | . |
| T4218 | 171-174 | DT | denotes | The |
| T4219 | 175-180 | NNS | denotes | tubes |
| T4220 | 181-191 | VBG | denotes | containing |
| T4221 | 192-199 | NNS | denotes | pellets |
| T4222 | 200-204 | VBD | denotes | were |
| T4223 | 205-208 | VBN | denotes | put |
| T4224 | 209-211 | IN | denotes | on |
| T4225 | 212-218 | NN | denotes | vortex |
| T4226 | 219-222 | IN | denotes | for |
| T4227 | 223-225 | CD | denotes | 15 |
| T4228 | 226-233 | NNS | denotes | seconds |
| T4229 | 234-239 | DT | denotes | every |
| T4230 | 240-242 | CD | denotes | 10 |
| T4231 | 243-250 | NNS | denotes | minutes |
| T4232 | 250-251 | , | denotes | , |
| T4233 | 252-255 | IN | denotes | for |
| T4234 | 256-257 | DT | denotes | a |
| T4235 | 258-263 | NN | denotes | total |
| T4236 | 264-266 | IN | denotes | of |
| T4237 | 267-269 | CD | denotes | 40 |
| T4238 | 270-277 | NNS | denotes | minutes |
| T4239 | 277-278 | . | denotes | . |
| T4240 | 279-282 | DT | denotes | The |
| T4241 | 283-288 | NNS | denotes | tubes |
| T4242 | 289-293 | VBD | denotes | were |
| T4243 | 294-305 | VBN | denotes | centrifuged |
| T4244 | 306-308 | IN | denotes | at |
| T4245 | 309-316 | JJ | denotes | maximum |
| T4246 | 317-322 | NN | denotes | speed |
| T4247 | 323-324 | -LRB- | denotes | ( |
| T4248 | 324-325 | FW | denotes | ~ |
| T4249 | 325-331 | CD | denotes | 16,000 |
| T4250 | 332-333 | CD | denotes | × |
| T4251 | 334-335 | NN | denotes | g |
| T4252 | 335-336 | -RRB- | denotes | ) |
| T4253 | 337-339 | IN | denotes | in |
| T4254 | 340-341 | DT | denotes | a |
| T4255 | 342-347 | NN | denotes | micro |
| T4256 | 348-358 | NN | denotes | centrifuge |
| T4257 | 359-362 | IN | denotes | for |
| T4258 | 363-365 | CD | denotes | 10 |
| T4259 | 366-373 | NNS | denotes | minutes |
| T4260 | 373-374 | . | denotes | . |
| T4261 | 375-378 | DT | denotes | The |
| T4262 | 379-391 | NNS | denotes | supernatants |
| T4263 | 392-393 | -LRB- | denotes | ( |
| T4264 | 393-400 | JJ | denotes | nuclear |
| T4265 | 401-408 | VB | denotes | extract |
| T4266 | 408-409 | -RRB- | denotes | ) |
| T4267 | 410-414 | VBD | denotes | were |
| T4268 | 415-426 | VBN | denotes | transferred |
| T4269 | 427-429 | TO | denotes | to |
| T4270 | 430-431 | DT | denotes | a |
| T4271 | 432-437 | JJ | denotes | clean |
| T4272 | 438-448 | JJ | denotes | prechilled |
| T4273 | 449-453 | NN | denotes | tube |
| T4274 | 454-457 | CC | denotes | and |
| T4275 | 458-464 | VBD | denotes | stored |
| T4276 | 465-467 | IN | denotes | at |
| T4277 | 468-469 | CD | denotes | − |
| T4278 | 469-471 | CD | denotes | 80 |
| T4279 | 471-472 | CD | denotes | ° |
| T4280 | 472-473 | NNP | denotes | C |
| T4281 | 474-479 | IN | denotes | until |
| T4282 | 480-483 | NN | denotes | use |
| T4283 | 483-484 | . | denotes | . |
| T4284 | 485-489 | NNP | denotes | EMSA |
| T4285 | 490-493 | VBD | denotes | was |
| T4286 | 494-503 | VBN | denotes | performed |
| T4287 | 504-506 | IN | denotes | as |
| T4288 | 507-516 | VBN | denotes | described |
| T4289 | 517-527 | RB | denotes | previously |
| T4290 | 528-529 | JJ | denotes | [ |
| T4291 | 529-530 | CD | denotes | 8 |
| T4292 | 530-531 | NN | denotes | ] |
| T4293 | 531-532 | . | denotes | . |
| T4294 | 533-537 | NNP | denotes | NFκB |
| T4295 | 538-545 | JJ | denotes | binding |
| T4296 | 546-554 | NN | denotes | activity |
| T4297 | 555-558 | VBD | denotes | was |
| T4298 | 559-567 | VBN | denotes | examined |
| T4299 | 568-570 | IN | denotes | in |
| T4300 | 571-572 | DT | denotes | a |
| T4301 | 573-575 | CD | denotes | 15 |
| T4302 | 576-578 | NN | denotes | μL |
| T4303 | 579-586 | JJ | denotes | binding |
| T4304 | 587-595 | NN | denotes | reaction |
| T4305 | 596-603 | NN | denotes | mixture |
| T4306 | 604-614 | VBG | denotes | containing |
| T4307 | 615-617 | CD | denotes | 15 |
| T4308 | 618-620 | NN | denotes | μg |
| T4309 | 621-623 | IN | denotes | of |
| T4310 | 624-631 | JJ | denotes | nuclear |
| T4311 | 632-640 | NNS | denotes | proteins |
| T4312 | 641-644 | CC | denotes | and |
| T4313 | 645-647 | CD | denotes | 35 |
| T4314 | 648-652 | NN | denotes | fmol |
| T4315 | 653-654 | NNP | denotes | [ |
| T4316 | 654-659 | NNP | denotes | γ-32P |
| T4317 | 659-660 | NNP | denotes | ] |
| T4318 | 661-668 | VBD | denotes | labeled |
| T4319 | 669-684 | JJ | denotes | double-stranded |
| T4320 | 685-689 | NNP | denotes | NFκB |
| T4321 | 690-699 | NN | denotes | consensus |
| T4322 | 700-715 | NN | denotes | oligonucleotide |
| T4323 | 715-716 | . | denotes | . |
| T4324 | 717-718 | DT | denotes | A |
| T4325 | 719-724 | JJ | denotes | super |
| T4326 | 725-730 | NN | denotes | shift |
| T4327 | 731-736 | NN | denotes | assay |
| T4328 | 737-742 | VBG | denotes | using |
| T4329 | 743-753 | NNS | denotes | antibodies |
| T4330 | 754-756 | TO | denotes | to |
| T4331 | 757-760 | NNP | denotes | P65 |
| T4332 | 761-764 | CC | denotes | and |
| T4333 | 765-768 | NNP | denotes | P50 |
| T4334 | 769-772 | VBD | denotes | was |
| T4335 | 773-782 | VBN | denotes | performed |
| T4336 | 783-785 | TO | denotes | to |
| T4337 | 786-793 | VB | denotes | confirm |
| T4338 | 794-798 | NNP | denotes | NFκB |
| T4339 | 799-806 | JJ | denotes | binding |
| T4340 | 807-818 | NN | denotes | specificity |
| T4341 | 819-821 | IN | denotes | as |
| T4342 | 822-832 | RB | denotes | previously |
| T4343 | 833-842 | VBN | denotes | described |
| T4344 | 842-843 | . | denotes | . |
| R3264 | T4190 | T4191 | compound | Nuclear,proteins |
| R3265 | T4191 | T4192 | nsubj | proteins,were |
| R3266 | T4192 | T4193 | auxpass | were,isolated |
| R3267 | T4193 | T4193 | ROOT | isolated,isolated |
| R3268 | T4194 | T4193 | prep | from,isolated |
| R3269 | T4195 | T4196 | compound | brain,tissue |
| R3270 | T4196 | T4194 | pobj | tissue,from |
| R3271 | T4197 | T4193 | punct | .,isolated |
| R3272 | T4198 | T4206 | advmod | Briefly,extract |
| R3273 | T4199 | T4206 | punct | ",",extract |
| R3274 | T4200 | T4206 | prep | after,extract |
| R3275 | T4201 | T4200 | pobj | removal,after |
| R3276 | T4202 | T4201 | prep | of,removal |
| R3277 | T4203 | T4202 | pobj | supernatant,of |
| R3278 | T4204 | T4206 | punct | (,extract |
| R3279 | T4205 | T4206 | nsubj | cytoplasmic,extract |
| R3280 | T4206 | T4206 | ROOT | extract,extract |
| R3281 | T4207 | T4206 | punct | ),extract |
| R3282 | T4208 | T4212 | punct | ",",resuspended |
| R3283 | T4209 | T4210 | det | the,pellets |
| R3284 | T4210 | T4212 | nsubjpass | pellets,resuspended |
| R3285 | T4211 | T4212 | auxpass | were,resuspended |
| R3286 | T4212 | T4212 | ROOT | resuspended,resuspended |
| R3287 | T4213 | T4212 | prep | in,resuspended |
| R3288 | T4214 | T4215 | amod | nuclear,extraction |
| R3289 | T4215 | T4213 | pobj | extraction,in |
| R3290 | T4216 | T4212 | conj | reagent,resuspended |
| R3291 | T4217 | T4212 | punct | .,resuspended |
| R3292 | T4218 | T4219 | det | The,tubes |
| R3293 | T4219 | T4223 | nsubjpass | tubes,put |
| R3294 | T4220 | T4219 | acl | containing,tubes |
| R3295 | T4221 | T4220 | dobj | pellets,containing |
| R3296 | T4222 | T4223 | auxpass | were,put |
| R3297 | T4223 | T4223 | ROOT | put,put |
| R3298 | T4224 | T4223 | prep | on,put |
| R3299 | T4225 | T4224 | pobj | vortex,on |
| R3300 | T4226 | T4223 | prep | for,put |
| R3301 | T4227 | T4228 | nummod | 15,seconds |
| R3302 | T4228 | T4226 | pobj | seconds,for |
| R3303 | T4229 | T4231 | det | every,minutes |
| R3304 | T4230 | T4231 | nummod | 10,minutes |
| R3305 | T4231 | T4223 | npadvmod | minutes,put |
| R3306 | T4232 | T4231 | punct | ",",minutes |
| R3307 | T4233 | T4231 | prep | for,minutes |
| R3308 | T4234 | T4235 | det | a,total |
| R3309 | T4235 | T4233 | pobj | total,for |
| R3310 | T4236 | T4235 | prep | of,total |
| R3311 | T4237 | T4238 | nummod | 40,minutes |
| R3312 | T4238 | T4236 | pobj | minutes,of |
| R3313 | T4239 | T4223 | punct | .,put |
| R3314 | T4240 | T4241 | det | The,tubes |
| R3315 | T4241 | T4243 | nsubjpass | tubes,centrifuged |
| R3316 | T4242 | T4243 | auxpass | were,centrifuged |
| R3317 | T4243 | T4243 | ROOT | centrifuged,centrifuged |
| R3318 | T4244 | T4243 | prep | at,centrifuged |
| R3319 | T4245 | T4246 | amod | maximum,speed |
| R3320 | T4246 | T4244 | pobj | speed,at |
| R3321 | T4247 | T4251 | punct | (,g |
| R3322 | T4248 | T4251 | nmod | ~,g |
| R3323 | T4249 | T4251 | dep | "16,000",g |
| R3324 | T4250 | T4251 | nummod | ×,g |
| R3325 | T4251 | T4246 | appos | g,speed |
| R3326 | T4252 | T4251 | punct | ),g |
| R3327 | T4253 | T4243 | prep | in,centrifuged |
| R3328 | T4254 | T4256 | det | a,centrifuge |
| R3329 | T4255 | T4256 | amod | micro,centrifuge |
| R3330 | T4256 | T4253 | pobj | centrifuge,in |
| R3331 | T4257 | T4243 | prep | for,centrifuged |
| R3332 | T4258 | T4259 | nummod | 10,minutes |
| R3333 | T4259 | T4257 | pobj | minutes,for |
| R3334 | T4260 | T4243 | punct | .,centrifuged |
| R3335 | T4261 | T4262 | det | The,supernatants |
| R3336 | T4262 | T4268 | nsubjpass | supernatants,transferred |
| R3337 | T4263 | T4262 | punct | (,supernatants |
| R3338 | T4264 | T4265 | amod | nuclear,extract |
| R3339 | T4265 | T4262 | appos | extract,supernatants |
| R3340 | T4266 | T4262 | punct | ),supernatants |
| R3341 | T4267 | T4268 | auxpass | were,transferred |
| R3342 | T4268 | T4268 | ROOT | transferred,transferred |
| R3343 | T4269 | T4268 | prep | to,transferred |
| R3344 | T4270 | T4273 | det | a,tube |
| R3345 | T4271 | T4273 | amod | clean,tube |
| R3346 | T4272 | T4273 | amod | prechilled,tube |
| R3347 | T4273 | T4269 | pobj | tube,to |
| R3348 | T4274 | T4268 | cc | and,transferred |
| R3349 | T4275 | T4268 | conj | stored,transferred |
| R3350 | T4276 | T4275 | prep | at,stored |
| R3351 | T4277 | T4279 | nummod | −,° |
| R3352 | T4278 | T4279 | nummod | 80,° |
| R3353 | T4279 | T4276 | pobj | °,at |
| R3354 | T4280 | T4275 | npadvmod | C,stored |
| R3355 | T4281 | T4275 | prep | until,stored |
| R3356 | T4282 | T4281 | pobj | use,until |
| R3357 | T4283 | T4268 | punct | .,transferred |
| R3358 | T4284 | T4286 | nsubjpass | EMSA,performed |
| R3359 | T4285 | T4286 | auxpass | was,performed |
| R3360 | T4286 | T4286 | ROOT | performed,performed |
| R3361 | T4287 | T4288 | mark | as,described |
| R3362 | T4288 | T4286 | advcl | described,performed |
| R3363 | T4289 | T4290 | advmod | previously,[ |
| R3364 | T4290 | T4292 | amod | [,] |
| R3365 | T4291 | T4292 | nummod | 8,] |
| R3366 | T4292 | T4288 | dobj | ],described |
| R3367 | T4293 | T4286 | punct | .,performed |
| R3368 | T4294 | T4296 | amod | NFκB,activity |
| R3369 | T4295 | T4296 | amod | binding,activity |
| R3370 | T4296 | T4298 | nsubjpass | activity,examined |
| R3371 | T4297 | T4298 | auxpass | was,examined |
| R3372 | T4298 | T4298 | ROOT | examined,examined |
| R3373 | T4299 | T4298 | prep | in,examined |
| R3374 | T4300 | T4305 | det | a,mixture |
| R3375 | T4301 | T4302 | nummod | 15,μL |
| R3376 | T4302 | T4305 | compound | μL,mixture |
| R3377 | T4303 | T4304 | amod | binding,reaction |
| R3378 | T4304 | T4305 | compound | reaction,mixture |
| R3379 | T4305 | T4299 | pobj | mixture,in |
| R3380 | T4306 | T4305 | acl | containing,mixture |
| R3381 | T4307 | T4308 | nummod | 15,μg |
| R3382 | T4308 | T4317 | nmod | μg,] |
| R3383 | T4309 | T4308 | prep | of,μg |
| R3384 | T4310 | T4311 | amod | nuclear,proteins |
| R3385 | T4311 | T4309 | pobj | proteins,of |
| R3386 | T4312 | T4311 | cc | and,proteins |
| R3387 | T4313 | T4314 | nummod | 35,fmol |
| R3388 | T4314 | T4317 | compound | fmol,] |
| R3389 | T4315 | T4317 | compound | [,] |
| R3390 | T4316 | T4317 | compound | γ-32P,] |
| R3391 | T4317 | T4306 | dobj | ],containing |
| R3392 | T4318 | T4298 | conj | labeled,examined |
| R3393 | T4319 | T4322 | amod | double-stranded,oligonucleotide |
| R3394 | T4320 | T4321 | compound | NFκB,consensus |
| R3395 | T4321 | T4322 | compound | consensus,oligonucleotide |
| R3396 | T4322 | T4318 | oprd | oligonucleotide,labeled |
| R3397 | T4323 | T4298 | punct | .,examined |
| R3398 | T4324 | T4327 | det | A,assay |
| R3399 | T4325 | T4326 | amod | super,shift |
| R3400 | T4326 | T4327 | compound | shift,assay |
| R3401 | T4327 | T4335 | nsubjpass | assay,performed |
| R3402 | T4328 | T4327 | acl | using,assay |
| R3403 | T4329 | T4328 | dobj | antibodies,using |
| R3404 | T4330 | T4328 | prep | to,using |
| R3405 | T4331 | T4330 | pobj | P65,to |
| R3406 | T4332 | T4331 | cc | and,P65 |
| R3407 | T4333 | T4331 | conj | P50,P65 |
| R3408 | T4334 | T4335 | auxpass | was,performed |
| R3409 | T4335 | T4335 | ROOT | performed,performed |
| R3410 | T4336 | T4337 | aux | to,confirm |
| R3411 | T4337 | T4335 | xcomp | confirm,performed |
| R3412 | T4338 | T4340 | nmod | NFκB,specificity |
| R3413 | T4339 | T4340 | amod | binding,specificity |
| R3414 | T4340 | T4337 | dobj | specificity,confirm |
| R3415 | T4341 | T4343 | mark | as,described |
| R3416 | T4342 | T4343 | advmod | previously,described |
| R3417 | T4343 | T4340 | advcl | described,specificity |
| R3418 | T4344 | T4335 | punct | .,performed |