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PMC:2664230 / 8811-10800 JSONTXT 17 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4152 0-8 NNP denotes SDS-PAGE
T4153 9-12 CC denotes and
T4154 13-27 NNP denotes Immunoblotting
T4155 28-29 -LRB- denotes (
T4156 29-34 NNP denotes I-κBα
T4157 34-35 , denotes ,
T4158 36-41 NNP denotes NF-κB
T4159 42-45 CD denotes p65
T4160 45-46 , denotes ,
T4161 47-50 CC denotes and
T4162 51-55 NNP denotes CREB
T4163 55-56 -RRB- denotes )
T4164 57-66 VBG denotes Following
T4165 67-78 NN denotes stimulation
T4166 78-79 , denotes ,
T4167 80-85 NNS denotes PBMCs
T4168 86-90 VBD denotes were
T4169 91-97 VBN denotes washed
T4170 98-102 IN denotes with
T4171 103-111 JJ denotes ice-cold
T4172 112-115 NNP denotes PBS
T4173 116-119 CC denotes and
T4174 120-131 VBD denotes centrifuged
T4175 132-134 TO denotes to
T4176 135-142 VB denotes collect
T4177 143-144 DT denotes a
T4178 145-149 NN denotes cell
T4179 150-156 NN denotes pellet
T4180 156-157 . denotes .
T4181 158-161 DT denotes The
T4182 162-168 NN denotes pellet
T4183 169-172 VBD denotes was
T4184 173-184 VBN denotes resuspended
T4185 185-187 IN denotes in
T4186 188-196 JJ denotes ice-cold
T4187 197-200 NNP denotes SDS
T4188 201-207 NN denotes sample
T4189 208-214 NN denotes buffer
T4190 215-227 VBN denotes supplemented
T4191 228-232 IN denotes with
T4192 233-236 CD denotes 100
T4193 237-239 NNP denotes mM
T4194 240-254 NN denotes dithiothreitol
T4195 254-255 . denotes .
T4196 256-267 NNP denotes Cytoplasmic
T4197 268-271 CC denotes and
T4198 272-279 JJ denotes nuclear
T4199 280-288 NNS denotes extracts
T4200 289-293 VBD denotes were
T4201 294-302 VBN denotes isolated
T4202 303-307 IN denotes with
T4203 308-314 NNP denotes NE-PER
T4204 315-322 JJ denotes nuclear
T4205 323-326 CC denotes and
T4206 327-338 JJ denotes cytoplasmic
T4207 339-349 NN denotes extraction
T4208 350-358 VBZ denotes reagents
T4209 359-363 IN denotes with
T4210 364-366 CD denotes 1x
T4211 367-371 NNP denotes Halt
T4212 372-380 NNP denotes Protease
T4213 381-390 NNP denotes Inhibitor
T4214 391-399 NNP denotes Cocktail
T4215 400-409 VBG denotes according
T4216 410-412 TO denotes to
T4217 413-416 DT denotes the
T4218 417-429 NN denotes manufacturer
T4219 432-444 NNS denotes instructions
T4220 444-445 . denotes .
T4221 446-453 NN denotes Protein
T4222 454-468 NNS denotes concentrations
T4223 469-473 VBD denotes were
T4224 474-484 VBN denotes determined
T4225 485-489 IN denotes with
T4226 490-493 DT denotes the
T4227 494-497 NNP denotes BCA
T4228 498-505 NN denotes protein
T4229 506-513 NN denotes reagent
T4230 514-517 NN denotes kit
T4231 518-521 IN denotes for
T4232 522-526 DT denotes each
T4233 527-533 NN denotes sample
T4234 534-543 VBG denotes according
T4235 544-546 TO denotes to
T4236 547-548 DT denotes a
T4237 549-561 JJ denotes standardized
T4238 562-567 NN denotes curve
T4239 568-571 IN denotes for
T4240 572-579 NN denotes albumin
T4241 579-580 . denotes .
T4242 581-585 NN denotes Cell
T4243 586-593 NNS denotes lysates
T4244 594-598 VBD denotes were
T4245 599-608 VBN denotes collected
T4246 609-611 IN denotes by
T4247 612-619 VBG denotes boiling
T4248 620-623 DT denotes the
T4249 624-631 NNS denotes samples
T4250 632-634 IN denotes in
T4251 635-636 DT denotes a
T4252 637-642 JJ denotes 100ºC
T4253 643-648 NN denotes water
T4254 649-653 NN denotes bath
T4255 654-657 IN denotes for
T4256 658-659 CD denotes 5
T4257 660-667 NNS denotes minutes
T4258 667-668 . denotes .
T4259 669-672 CD denotes Ten
T4260 673-675 NN denotes μg
T4261 676-678 IN denotes of
T4262 679-686 NN denotes protein
T4263 687-690 IN denotes per
T4264 691-697 NN denotes sample
T4265 698-701 VBD denotes was
T4266 702-711 VBN denotes separated
T4267 712-714 IN denotes by
T4268 715-733 JJ denotes SDS-polyacrylamide
T4269 734-737 NN denotes gel
T4270 738-753 NN denotes electrophoresis
T4271 754-761 IN denotes through
T4272 762-763 CD denotes 8
T4273 764-766 CD denotes 16
T4274 766-767 NN denotes %
T4275 768-780 JJ denotes tris-glycine
T4276 781-795 NN denotes polyacrylamide
T4277 796-804 NN denotes gradient
T4278 805-809 NNS denotes gels
T4279 810-813 CC denotes and
T4280 814-825 VBN denotes transferred
T4281 826-828 TO denotes to
T4282 829-843 JJ denotes nitrocellulose
T4283 844-853 NNS denotes membranes
T4284 853-854 . denotes .
T4285 855-858 DT denotes The
T4286 859-868 NNS denotes membranes
T4287 869-873 VBD denotes were
T4288 874-881 VBN denotes blocked
T4289 882-886 IN denotes with
T4290 887-888 CD denotes 5
T4291 888-889 NN denotes %
T4292 890-894 NN denotes milk
T4293 895-897 IN denotes in
T4294 898-911 JJ denotes Tris-buffered
T4295 912-924 NNP denotes saline/Tween
T4296 925-927 CD denotes 20
T4297 928-929 -LRB- denotes (
T4298 929-936 NNP denotes Fischer
T4299 937-947 NNP denotes Scientific
T4300 947-948 , denotes ,
T4301 949-959 NNP denotes Pittsburgh
T4302 959-960 , denotes ,
T4303 961-963 NNP denotes PA
T4304 963-964 -RRB- denotes )
T4305 965-968 IN denotes for
T4306 969-970 CD denotes 1
T4307 971-975 NN denotes hour
T4308 975-976 . denotes .
T4309 977-988 JJ denotes Cytoplasmic
T4310 989-997 NNS denotes extracts
T4311 998-1002 VBD denotes were
T4312 1003-1012 VBN denotes incubated
T4313 1013-1017 IN denotes with
T4314 1018-1032 JJ denotes phosphorylated
T4315 1033-1038 JJ denotes I-κBα
T4316 1039-1047 NN denotes antibody
T4317 1048-1049 -LRB- denotes (
T4318 1049-1054 CD denotes 1:200
T4319 1054-1055 -RRB- denotes )
T4320 1055-1056 , denotes ,
T4321 1057-1062 IN denotes while
T4322 1063-1070 JJ denotes nuclear
T4323 1071-1079 NNS denotes extracts
T4324 1080-1084 VBD denotes were
T4325 1085-1094 VBN denotes incubated
T4326 1095-1099 IN denotes with
T4327 1100-1106 DT denotes either
T4328 1107-1121 VBN denotes phosphorylated
T4329 1122-1127 NNP denotes NF-κB
T4330 1128-1131 CD denotes p65
T4331 1132-1140 NN denotes antibody
T4332 1141-1142 -LRB- denotes (
T4333 1142-1147 CD denotes 1:500
T4334 1147-1148 -RRB- denotes )
T4335 1149-1151 CC denotes or
T4336 1152-1166 VBN denotes phosphorylated
T4337 1167-1171 NNP denotes CREB
T4338 1172-1180 NN denotes antibody
T4339 1181-1182 -LRB- denotes (
T4340 1182-1187 CD denotes 1:500
T4341 1187-1188 -RRB- denotes )
T4342 1189-1198 RB denotes overnight
T4343 1199-1201 IN denotes at
T4344 1202-1203 CD denotes 4
T4345 1203-1204 CD denotes °
T4346 1204-1205 NNP denotes C
T4347 1206-1208 IN denotes in
T4348 1209-1217 JJ denotes separate
T4349 1218-1229 NNS denotes experiments
T4350 1229-1230 . denotes .
T4351 1231-1234 DT denotes The
T4352 1235-1244 NNS denotes membranes
T4353 1245-1249 VBD denotes were
T4354 1250-1256 VBN denotes washed
T4355 1257-1261 IN denotes with
T4356 1262-1275 JJ denotes Tris-buffered
T4357 1276-1288 NNP denotes saline/Tween
T4358 1289-1291 CD denotes 20
T4359 1292-1295 CC denotes and
T4360 1296-1305 VBD denotes incubated
T4361 1306-1309 IN denotes for
T4362 1310-1311 CD denotes 1
T4363 1312-1316 NN denotes hour
T4364 1317-1319 IN denotes at
T4365 1320-1324 NN denotes room
T4366 1325-1336 NN denotes temperature
T4367 1337-1341 IN denotes with
T4368 1342-1345 DT denotes the
T4369 1346-1355 JJ denotes secondary
T4370 1356-1364 NN denotes antibody
T4371 1364-1365 , denotes ,
T4372 1366-1377 JJ denotes horseradish
T4373 1378-1395 JJ denotes peroxidase-linked
T4374 1396-1407 JJ denotes anti-rabbit
T4375 1408-1411 NNP denotes IgG
T4376 1412-1419 VBD denotes diluted
T4377 1420-1426 CD denotes 1:2000
T4378 1427-1429 IN denotes in
T4379 1430-1438 VBG denotes blocking
T4380 1439-1447 NN denotes solution
T4381 1447-1448 . denotes .
T4382 1449-1454 IN denotes After
T4383 1455-1463 VBN denotes repeated
T4384 1464-1471 VBG denotes washing
T4385 1472-1474 IN denotes of
T4386 1475-1478 DT denotes the
T4387 1479-1487 NN denotes membrane
T4388 1487-1488 , denotes ,
T4389 1489-1492 DT denotes the
T4390 1493-1504 NNP denotes Supersignal
T4391 1505-1509 NNP denotes West
T4392 1510-1514 NNP denotes Pico
T4393 1515-1531 NNP denotes Chemiluminescent
T4394 1532-1535 NNP denotes Kit
T4395 1536-1539 VBD denotes was
T4396 1540-1547 VBN denotes applied
T4397 1548-1551 IN denotes for
T4398 1552-1560 NN denotes antibody
T4399 1561-1570 NN denotes detection
T4400 1571-1574 IN denotes per
T4401 1575-1578 DT denotes the
T4402 1579-1591 NN denotes manufacturer
T4403 1594-1606 NNS denotes instructions
T4404 1606-1607 . denotes .
T4405 1608-1615 JJ denotes Western
T4406 1616-1620 NN denotes blot
T4407 1621-1625 NNS denotes data
T4408 1626-1628 VBZ denotes is
T4409 1629-1638 VBN denotes presented
T4410 1639-1641 IN denotes as
T4411 1642-1643 DT denotes a
T4412 1644-1654 NN denotes percentage
T4413 1655-1657 IN denotes of
T4414 1658-1661 NNP denotes LPS
T4415 1662-1673 NN denotes stimulation
T4416 1673-1674 . denotes .
T4417 1675-1678 DT denotes The
T4418 1679-1689 NN denotes percentage
T4419 1690-1692 IN denotes of
T4420 1693-1696 NNP denotes LPS
T4421 1697-1708 NN denotes stimulation
T4422 1709-1712 VBD denotes was
T4423 1713-1723 VBN denotes calculated
T4424 1724-1726 IN denotes by
T4425 1727-1735 VBG denotes dividing
T4426 1736-1739 DT denotes the
T4427 1740-1744 JJ denotes mean
T4428 1745-1749 NN denotes band
T4429 1750-1755 NN denotes pixel
T4430 1756-1761 NN denotes total
T4431 1762-1765 IN denotes for
T4432 1766-1770 DT denotes each
T4433 1771-1780 NN denotes treatment
T4434 1781-1784 NN denotes arm
T4435 1785-1792 VBN denotes divided
T4436 1793-1795 IN denotes by
T4437 1796-1799 DT denotes the
T4438 1800-1804 JJ denotes mean
T4439 1805-1810 NN denotes pixel
T4440 1811-1816 NN denotes total
T4441 1817-1819 IN denotes of
T4442 1820-1827 NNS denotes samples
T4443 1828-1838 VBN denotes stimulated
T4444 1839-1843 IN denotes with
T4445 1844-1847 NNP denotes LPS
T4446 1848-1851 CC denotes and
T4447 1852-1863 VBG denotes multiplying
T4448 1864-1866 IN denotes by
T4449 1867-1870 CD denotes 100
T4450 1870-1871 . denotes .
T4451 1872-1876 RB denotes Thus
T4452 1876-1877 , denotes ,
T4453 1878-1881 NNP denotes LPS
T4454 1882-1893 NN denotes stimulation
T4455 1894-1896 VBZ denotes is
T4456 1897-1905 VBN denotes reported
T4457 1906-1908 IN denotes as
T4458 1909-1912 CD denotes 100
T4459 1912-1913 NN denotes %
T4460 1914-1917 CC denotes and
T4461 1918-1922 DT denotes each
T4462 1923-1925 IN denotes of
T4463 1926-1929 DT denotes the
T4464 1930-1939 NN denotes treatment
T4465 1940-1944 NNS denotes arms
T4466 1945-1947 VBZ denotes is
T4467 1948-1956 VBN denotes reported
T4468 1957-1959 IN denotes as
T4469 1960-1961 DT denotes a
T4470 1962-1969 NN denotes percent
T4471 1970-1972 IN denotes of
T4472 1973-1976 NNP denotes LPS
T4473 1977-1988 NN denotes stimulation
T4474 1988-1989 . denotes .
R3373 T4152 T4152 ROOT SDS-PAGE,SDS-PAGE
R3374 T4153 T4152 cc and,SDS-PAGE
R3375 T4154 T4152 conj Immunoblotting,SDS-PAGE
R3376 T4155 T4156 punct (,I-κBα
R3377 T4156 T4152 conj I-κBα,SDS-PAGE
R3378 T4157 T4156 punct ",",I-κBα
R3379 T4158 T4156 conj NF-κB,I-κBα
R3380 T4159 T4156 appos p65,I-κBα
R3381 T4160 T4156 punct ",",I-κBα
R3382 T4161 T4156 cc and,I-κBα
R3383 T4162 T4156 conj CREB,I-κBα
R3384 T4163 T4156 punct ),I-κBα
R3385 T4164 T4169 prep Following,washed
R3386 T4165 T4164 pobj stimulation,Following
R3387 T4166 T4169 punct ",",washed
R3388 T4167 T4169 nsubjpass PBMCs,washed
R3389 T4168 T4169 auxpass were,washed
R3390 T4169 T4169 ROOT washed,washed
R3391 T4170 T4169 prep with,washed
R3392 T4171 T4172 amod ice-cold,PBS
R3393 T4172 T4170 pobj PBS,with
R3394 T4173 T4169 cc and,washed
R3395 T4174 T4169 conj centrifuged,washed
R3396 T4175 T4176 aux to,collect
R3397 T4176 T4174 advcl collect,centrifuged
R3398 T4177 T4179 det a,pellet
R3399 T4178 T4179 compound cell,pellet
R3400 T4179 T4176 dobj pellet,collect
R3401 T4180 T4169 punct .,washed
R3402 T4181 T4182 det The,pellet
R3403 T4182 T4184 nsubjpass pellet,resuspended
R3404 T4183 T4184 auxpass was,resuspended
R3405 T4184 T4184 ROOT resuspended,resuspended
R3406 T4185 T4184 prep in,resuspended
R3407 T4186 T4189 amod ice-cold,buffer
R3408 T4187 T4189 compound SDS,buffer
R3409 T4188 T4189 compound sample,buffer
R3410 T4189 T4185 pobj buffer,in
R3411 T4190 T4184 conj supplemented,resuspended
R3412 T4191 T4190 prep with,supplemented
R3413 T4192 T4194 nummod 100,dithiothreitol
R3414 T4193 T4194 compound mM,dithiothreitol
R3415 T4194 T4191 pobj dithiothreitol,with
R3416 T4195 T4184 punct .,resuspended
R3417 T4196 T4200 nsubj Cytoplasmic,were
R3418 T4197 T4196 cc and,Cytoplasmic
R3419 T4198 T4199 amod nuclear,extracts
R3420 T4199 T4196 conj extracts,Cytoplasmic
R3421 T4200 T4200 ROOT were,were
R3422 T4201 T4200 acomp isolated,were
R3423 T4202 T4201 prep with,isolated
R3424 T4203 T4208 compound NE-PER,reagents
R3425 T4204 T4207 amod nuclear,extraction
R3426 T4205 T4204 cc and,nuclear
R3427 T4206 T4204 conj cytoplasmic,nuclear
R3428 T4207 T4208 compound extraction,reagents
R3429 T4208 T4202 pobj reagents,with
R3430 T4209 T4208 prep with,reagents
R3431 T4210 T4214 nummod 1x,Cocktail
R3432 T4211 T4214 compound Halt,Cocktail
R3433 T4212 T4214 compound Protease,Cocktail
R3434 T4213 T4214 compound Inhibitor,Cocktail
R3435 T4214 T4215 nsubj Cocktail,according
R3436 T4215 T4200 prep according,were
R3437 T4216 T4215 prep to,according
R3438 T4217 T4218 det the,manufacturer
R3439 T4218 T4219 poss manufacturer,instructions
R3440 T4219 T4216 pobj instructions,to
R3441 T4220 T4200 punct .,were
R3442 T4221 T4222 compound Protein,concentrations
R3443 T4222 T4224 nsubjpass concentrations,determined
R3444 T4223 T4224 auxpass were,determined
R3445 T4224 T4224 ROOT determined,determined
R3446 T4225 T4224 prep with,determined
R3447 T4226 T4228 det the,protein
R3448 T4227 T4228 compound BCA,protein
R3449 T4228 T4230 nmod protein,kit
R3450 T4229 T4230 compound reagent,kit
R3451 T4230 T4225 pobj kit,with
R3452 T4231 T4230 prep for,kit
R3453 T4232 T4233 det each,sample
R3454 T4233 T4231 pobj sample,for
R3455 T4234 T4224 prep according,determined
R3456 T4235 T4234 prep to,according
R3457 T4236 T4238 det a,curve
R3458 T4237 T4238 amod standardized,curve
R3459 T4238 T4235 pobj curve,to
R3460 T4239 T4238 prep for,curve
R3461 T4240 T4239 pobj albumin,for
R3462 T4241 T4224 punct .,determined
R3463 T4242 T4243 compound Cell,lysates
R3464 T4243 T4245 nsubjpass lysates,collected
R3465 T4244 T4245 auxpass were,collected
R3466 T4245 T4245 ROOT collected,collected
R3467 T4246 T4245 prep by,collected
R3468 T4247 T4246 pcomp boiling,by
R3469 T4248 T4249 det the,samples
R3470 T4249 T4247 dobj samples,boiling
R3471 T4250 T4247 prep in,boiling
R3472 T4251 T4254 det a,bath
R3473 T4252 T4254 amod 100ºC,bath
R3474 T4253 T4254 compound water,bath
R3475 T4254 T4250 pobj bath,in
R3476 T4255 T4247 prep for,boiling
R3477 T4256 T4257 nummod 5,minutes
R3478 T4257 T4255 pobj minutes,for
R3479 T4258 T4245 punct .,collected
R3480 T4259 T4260 compound Ten,μg
R3481 T4260 T4266 nsubjpass μg,separated
R3482 T4261 T4260 prep of,μg
R3483 T4262 T4261 pobj protein,of
R3484 T4263 T4262 prep per,protein
R3485 T4264 T4263 pobj sample,per
R3486 T4265 T4266 auxpass was,separated
R3487 T4266 T4266 ROOT separated,separated
R3488 T4267 T4266 agent by,separated
R3489 T4268 T4270 amod SDS-polyacrylamide,electrophoresis
R3490 T4269 T4270 compound gel,electrophoresis
R3491 T4270 T4267 pobj electrophoresis,by
R3492 T4271 T4266 prep through,separated
R3493 T4272 T4271 pobj 8,through
R3494 T4273 T4274 nummod 16,%
R3495 T4274 T4278 nmod %,gels
R3496 T4275 T4278 amod tris-glycine,gels
R3497 T4276 T4278 compound polyacrylamide,gels
R3498 T4277 T4278 compound gradient,gels
R3499 T4278 T4272 appos gels,8
R3500 T4279 T4266 cc and,separated
R3501 T4280 T4266 conj transferred,separated
R3502 T4281 T4280 prep to,transferred
R3503 T4282 T4283 amod nitrocellulose,membranes
R3504 T4283 T4281 pobj membranes,to
R3505 T4284 T4266 punct .,separated
R3506 T4285 T4286 det The,membranes
R3507 T4286 T4288 nsubjpass membranes,blocked
R3508 T4287 T4288 auxpass were,blocked
R3509 T4288 T4288 ROOT blocked,blocked
R3510 T4289 T4288 prep with,blocked
R3511 T4290 T4291 nummod 5,%
R3512 T4291 T4292 compound %,milk
R3513 T4292 T4289 pobj milk,with
R3514 T4293 T4292 prep in,milk
R3515 T4294 T4295 compound Tris-buffered,saline/Tween
R3516 T4295 T4293 pobj saline/Tween,in
R3517 T4296 T4295 nummod 20,saline/Tween
R3518 T4297 T4303 punct (,PA
R3519 T4298 T4299 compound Fischer,Scientific
R3520 T4299 T4303 dep Scientific,PA
R3521 T4300 T4299 punct ",",Scientific
R3522 T4301 T4299 appos Pittsburgh,Scientific
R3523 T4302 T4301 punct ",",Pittsburgh
R3524 T4303 T4303 ROOT PA,PA
R3525 T4304 T4303 punct ),PA
R3526 T4305 T4303 prep for,PA
R3527 T4306 T4307 nummod 1,hour
R3528 T4307 T4305 pobj hour,for
R3529 T4308 T4303 punct .,PA
R3530 T4309 T4310 amod Cytoplasmic,extracts
R3531 T4310 T4312 nsubjpass extracts,incubated
R3532 T4311 T4312 auxpass were,incubated
R3533 T4312 T4312 ROOT incubated,incubated
R3534 T4313 T4312 prep with,incubated
R3535 T4314 T4316 amod phosphorylated,antibody
R3536 T4315 T4316 amod I-κBα,antibody
R3537 T4316 T4313 pobj antibody,with
R3538 T4317 T4316 punct (,antibody
R3539 T4318 T4316 appos 1:200,antibody
R3540 T4319 T4316 punct ),antibody
R3541 T4320 T4312 punct ",",incubated
R3542 T4321 T4325 mark while,incubated
R3543 T4322 T4323 amod nuclear,extracts
R3544 T4323 T4325 nsubjpass extracts,incubated
R3545 T4324 T4325 auxpass were,incubated
R3546 T4325 T4312 advcl incubated,incubated
R3547 T4326 T4325 prep with,incubated
R3548 T4327 T4331 det either,antibody
R3549 T4328 T4331 amod phosphorylated,antibody
R3550 T4329 T4331 nmod NF-κB,antibody
R3551 T4330 T4331 nummod p65,antibody
R3552 T4331 T4326 pobj antibody,with
R3553 T4332 T4331 punct (,antibody
R3554 T4333 T4331 appos 1:500,antibody
R3555 T4334 T4331 punct ),antibody
R3556 T4335 T4325 cc or,incubated
R3557 T4336 T4338 amod phosphorylated,antibody
R3558 T4337 T4338 compound CREB,antibody
R3559 T4338 T4346 nmod antibody,C
R3560 T4339 T4338 punct (,antibody
R3561 T4340 T4338 appos 1:500,antibody
R3562 T4341 T4338 punct ),antibody
R3563 T4342 T4346 amod overnight,C
R3564 T4343 T4342 prep at,overnight
R3565 T4344 T4345 nummod 4,°
R3566 T4345 T4343 pobj °,at
R3567 T4346 T4325 conj C,incubated
R3568 T4347 T4346 prep in,C
R3569 T4348 T4349 amod separate,experiments
R3570 T4349 T4347 pobj experiments,in
R3571 T4350 T4312 punct .,incubated
R3572 T4351 T4352 det The,membranes
R3573 T4352 T4354 nsubjpass membranes,washed
R3574 T4353 T4354 auxpass were,washed
R3575 T4354 T4354 ROOT washed,washed
R3576 T4355 T4354 prep with,washed
R3577 T4356 T4357 compound Tris-buffered,saline/Tween
R3578 T4357 T4355 pobj saline/Tween,with
R3579 T4358 T4357 nummod 20,saline/Tween
R3580 T4359 T4354 cc and,washed
R3581 T4360 T4354 conj incubated,washed
R3582 T4361 T4360 prep for,incubated
R3583 T4362 T4363 nummod 1,hour
R3584 T4363 T4361 pobj hour,for
R3585 T4364 T4360 prep at,incubated
R3586 T4365 T4366 compound room,temperature
R3587 T4366 T4364 pobj temperature,at
R3588 T4367 T4360 prep with,incubated
R3589 T4368 T4370 det the,antibody
R3590 T4369 T4370 amod secondary,antibody
R3591 T4370 T4367 pobj antibody,with
R3592 T4371 T4376 punct ",",diluted
R3593 T4372 T4375 nmod horseradish,IgG
R3594 T4373 T4375 amod peroxidase-linked,IgG
R3595 T4374 T4375 compound anti-rabbit,IgG
R3596 T4375 T4376 nsubj IgG,diluted
R3597 T4376 T4354 conj diluted,washed
R3598 T4377 T4376 dobj 1:2000,diluted
R3599 T4378 T4376 prep in,diluted
R3600 T4379 T4378 pcomp blocking,in
R3601 T4380 T4379 dobj solution,blocking
R3602 T4381 T4376 punct .,diluted
R3603 T4382 T4396 prep After,applied
R3604 T4383 T4384 amod repeated,washing
R3605 T4384 T4382 pobj washing,After
R3606 T4385 T4384 prep of,washing
R3607 T4386 T4387 det the,membrane
R3608 T4387 T4385 pobj membrane,of
R3609 T4388 T4396 punct ",",applied
R3610 T4389 T4394 det the,Kit
R3611 T4390 T4394 compound Supersignal,Kit
R3612 T4391 T4392 compound West,Pico
R3613 T4392 T4394 compound Pico,Kit
R3614 T4393 T4394 compound Chemiluminescent,Kit
R3615 T4394 T4396 nsubjpass Kit,applied
R3616 T4395 T4396 auxpass was,applied
R3617 T4396 T4396 ROOT applied,applied
R3618 T4397 T4396 prep for,applied
R3619 T4398 T4399 compound antibody,detection
R3620 T4399 T4397 pobj detection,for
R3621 T4400 T4399 prep per,detection
R3622 T4401 T4402 det the,manufacturer
R3623 T4402 T4403 poss manufacturer,instructions
R3624 T4403 T4400 pobj instructions,per
R3625 T4404 T4396 punct .,applied
R3626 T4405 T4407 amod Western,data
R3627 T4406 T4407 compound blot,data
R3628 T4407 T4409 nsubjpass data,presented
R3629 T4408 T4409 auxpass is,presented
R3630 T4409 T4409 ROOT presented,presented
R3631 T4410 T4409 prep as,presented
R3632 T4411 T4412 det a,percentage
R3633 T4412 T4410 pobj percentage,as
R3634 T4413 T4412 prep of,percentage
R3635 T4414 T4415 compound LPS,stimulation
R3636 T4415 T4413 pobj stimulation,of
R3637 T4416 T4409 punct .,presented
R3638 T4417 T4418 det The,percentage
R3639 T4418 T4423 nsubjpass percentage,calculated
R3640 T4419 T4418 prep of,percentage
R3641 T4420 T4421 compound LPS,stimulation
R3642 T4421 T4419 pobj stimulation,of
R3643 T4422 T4423 auxpass was,calculated
R3644 T4423 T4423 ROOT calculated,calculated
R3645 T4424 T4423 prep by,calculated
R3646 T4425 T4424 pcomp dividing,by
R3647 T4426 T4430 det the,total
R3648 T4427 T4430 amod mean,total
R3649 T4428 T4429 compound band,pixel
R3650 T4429 T4430 compound pixel,total
R3651 T4430 T4425 dobj total,dividing
R3652 T4431 T4430 prep for,total
R3653 T4432 T4434 det each,arm
R3654 T4433 T4434 compound treatment,arm
R3655 T4434 T4431 pobj arm,for
R3656 T4435 T4430 acl divided,total
R3657 T4436 T4435 agent by,divided
R3658 T4437 T4440 det the,total
R3659 T4438 T4440 amod mean,total
R3660 T4439 T4440 amod pixel,total
R3661 T4440 T4436 pobj total,by
R3662 T4441 T4440 prep of,total
R3663 T4442 T4441 pobj samples,of
R3664 T4443 T4442 acl stimulated,samples
R3665 T4444 T4443 prep with,stimulated
R3666 T4445 T4444 pobj LPS,with
R3667 T4446 T4443 cc and,stimulated
R3668 T4447 T4425 advcl multiplying,dividing
R3669 T4448 T4447 prep by,multiplying
R3670 T4449 T4448 pobj 100,by
R3671 T4450 T4423 punct .,calculated
R3672 T4451 T4456 advmod Thus,reported
R3673 T4452 T4456 punct ",",reported
R3674 T4453 T4454 compound LPS,stimulation
R3675 T4454 T4456 nsubjpass stimulation,reported
R3676 T4455 T4456 auxpass is,reported
R3677 T4456 T4456 ROOT reported,reported
R3678 T4457 T4456 prep as,reported
R3679 T4458 T4459 nummod 100,%
R3680 T4459 T4457 pobj %,as
R3681 T4460 T4456 cc and,reported
R3682 T4461 T4467 nsubjpass each,reported
R3683 T4462 T4461 prep of,each
R3684 T4463 T4465 det the,arms
R3685 T4464 T4465 compound treatment,arms
R3686 T4465 T4462 pobj arms,of
R3687 T4466 T4467 auxpass is,reported
R3688 T4467 T4456 conj reported,reported
R3689 T4468 T4467 prep as,reported
R3690 T4469 T4470 det a,percent
R3691 T4470 T4468 pobj percent,as
R3692 T4471 T4470 prep of,percent
R3693 T4472 T4473 compound LPS,stimulation
R3694 T4473 T4471 pobj stimulation,of
R3695 T4474 T4467 punct .,reported