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PMC:1310901 / 5129-13048 JSONTXT 18 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2774 23-27 NN denotes Cell
T2775 28-33 NNS denotes lines
T2776 34-39 NNP denotes K-562
T2777 39-40 , denotes ,
T2778 41-47 NNP denotes Jurkat
T2779 48-51 CC denotes and
T2780 52-57 NNP denotes U-937
T2781 58-62 VBD denotes were
T2782 63-71 VBN denotes obtained
T2783 72-76 IN denotes from
T2784 77-80 DT denotes the
T2785 81-85 NNP denotes ATCC
T2786 86-87 -LRB- denotes (
T2787 87-95 NNP denotes American
T2788 96-100 NNP denotes Type
T2789 101-108 NNP denotes Culture
T2790 109-119 NNP denotes Collection
T2791 119-120 , denotes ,
T2792 121-130 NNP denotes Rockville
T2793 130-131 , denotes ,
T2794 132-135 NNP denotes USA
T2795 135-136 -RRB- denotes )
T2796 137-140 CC denotes and
T2797 141-145 NNP denotes EM-2
T2798 145-146 , denotes ,
T2799 147-154 NNP denotes LAMA-84
T2800 154-155 , denotes ,
T2801 156-162 NNP denotes CML-T1
T2802 162-163 , denotes ,
T2803 164-170 NNP denotes BV-173
T2804 170-171 , denotes ,
T2805 172-176 NNP denotes SD-1
T2806 177-180 CC denotes and
T2807 181-190 NNP denotes RPMI-8226
T2808 191-195 IN denotes from
T2809 196-199 DT denotes the
T2810 200-204 NNP denotes DSMZ
T2811 205-206 -LRB- denotes (
T2812 206-214 NNP denotes Deutsche
T2813 215-223 NNP denotes Sammlung
T2814 224-227 NNP denotes von
T2815 228-243 NNP denotes Mikroorganismen
T2816 244-247 NN denotes und
T2817 248-260 NNP denotes Zellkulturen
T2818 261-265 NNP denotes GmbH
T2819 265-266 , denotes ,
T2820 267-279 NNP denotes Braunschweig
T2821 279-280 , denotes ,
T2822 281-288 NNP denotes Germany
T2823 288-289 -RRB- denotes )
T2824 289-290 . denotes .
T2825 291-294 DT denotes All
T2826 295-299 NN denotes cell
T2827 300-305 NNS denotes lines
T2828 305-306 , denotes ,
T2829 307-313 IN denotes except
T2830 314-320 NNP denotes BV-173
T2831 320-321 , denotes ,
T2832 322-326 NNP denotes SD-1
T2833 327-330 CC denotes and
T2834 331-340 NNP denotes RPMI-8226
T2835 340-341 , denotes ,
T2836 342-346 VBD denotes were
T2837 347-361 JJ denotes IRF-4-negative
T2838 361-362 . denotes .
T2949 364-368 NNP denotes Cell
T2950 369-376 NN denotes culture
T2951 377-380 CC denotes and
T2952 381-392 NN denotes stimulation
T2953 393-396 DT denotes All
T2954 397-401 NN denotes cell
T2955 402-407 NNS denotes lines
T2956 408-412 VBD denotes were
T2957 413-423 VBN denotes maintained
T2958 424-426 IN denotes at
T2959 427-428 CD denotes 5
T2960 428-429 NN denotes %
T2961 430-433 CD denotes CO2
T2962 434-436 IN denotes in
T2963 437-441 NNP denotes RPMI
T2964 442-446 CD denotes 1640
T2965 447-453 NN denotes medium
T2966 454-458 IN denotes with
T2967 459-460 CD denotes 1
T2968 460-461 NN denotes %
T2969 462-471 NN denotes glutamine
T2970 472-473 -LRB- denotes (
T2971 473-482 NNP denotes Gibco/BRL
T2972 483-493 NNP denotes Eggenstein
T2973 493-494 , denotes ,
T2974 495-502 NNP denotes Germany
T2975 502-503 -RRB- denotes )
T2976 504-516 VBN denotes supplemented
T2977 517-521 IN denotes with
T2978 522-524 CD denotes 10
T2979 524-525 NN denotes %
T2980 526-531 JJ denotes fetal
T2981 532-536 NN denotes calf
T2982 537-542 NN denotes serum
T2983 543-544 -LRB- denotes (
T2984 544-553 NNP denotes Gibco/BRL
T2985 553-554 -RRB- denotes )
T2986 554-555 , denotes ,
T2987 556-557 CD denotes 1
T2988 557-558 NN denotes %
T2989 559-582 NN denotes penicillin/streptomycin
T2990 583-584 -LRB- denotes (
T2991 584-592 NNP denotes Biochrom
T2992 592-593 , denotes ,
T2993 594-600 NNP denotes Berlin
T2994 600-601 , denotes ,
T2995 602-609 NNP denotes Germany
T2996 609-610 -RRB- denotes )
T2997 610-611 . denotes .
T2998 612-616 WRB denotes When
T2999 617-626 VBN denotes indicated
T3000 626-627 , denotes ,
T3001 628-633 NNS denotes cells
T3002 634-638 VBD denotes were
T3003 639-646 VBN denotes treated
T3004 647-651 IN denotes with
T3005 652-673 JJ denotes 5-aza-2-deoxycytidine
T3006 674-675 -LRB- denotes (
T3007 675-680 NNP denotes AzadC
T3008 680-681 -RRB- denotes )
T3009 682-684 CC denotes or
T3010 685-698 JJ denotes 5-azacytidine
T3011 699-700 -LRB- denotes (
T3012 700-704 NNP denotes AzaC
T3013 704-705 -RRB- denotes )
T3014 706-707 -LRB- denotes (
T3015 707-712 NNP denotes Sigma
T3016 712-713 , denotes ,
T3017 714-725 NNP denotes Taufkirchen
T3018 725-726 , denotes ,
T3019 727-734 NNP denotes Germany
T3020 734-735 -RRB- denotes )
T3021 736-739 IN denotes for
T3022 740-749 JJ denotes different
T3023 750-754 NN denotes time
T3024 755-762 NNS denotes periods
T3025 762-763 . denotes .
T3026 764-769 VBG denotes Owing
T3027 770-772 TO denotes to
T3028 773-778 PRP$ denotes their
T3029 779-787 NN denotes chemical
T3030 788-799 NN denotes instability
T3031 800-805 JJ denotes fresh
T3032 806-816 NNS denotes substances
T3033 817-821 VBD denotes were
T3034 822-830 JJ denotes re-added
T3035 831-836 DT denotes every
T3036 837-839 CD denotes 24
T3037 840-842 NN denotes h.
T3192 844-854 VBG denotes Sequencing
T3193 855-857 IN denotes of
T3194 858-861 DT denotes the
T3195 862-867 NN denotes IRF-4
T3196 868-876 NN denotes promoter
T3197 877-880 IN denotes For
T3198 881-889 NN denotes analysis
T3199 890-892 IN denotes of
T3200 893-896 DT denotes the
T3201 897-902 NN denotes IRF-4
T3202 903-911 NN denotes promoter
T3203 912-918 NN denotes region
T3204 919-922 IN denotes for
T3205 923-932 JJ denotes permanent
T3206 933-944 NNS denotes aberrations
T3207 945-949 JJ denotes such
T3208 950-952 IN denotes as
T3209 953-973 NNS denotes insertions/deletions
T3210 974-976 CC denotes or
T3211 977-985 NN denotes mutation
T3212 985-986 , denotes ,
T3213 987-989 PRP denotes we
T3214 990-1003 VBD denotes PCR-amplified
T3215 1004-1007 CD denotes two
T3216 1008-1017 NNS denotes fragments
T3217 1018-1022 IN denotes from
T3218 1023-1030 JJ denotes genomic
T3219 1031-1034 NN denotes DNA
T3220 1034-1035 , denotes ,
T3221 1036-1041 WDT denotes which
T3222 1042-1045 VBD denotes was
T3223 1046-1055 VBN denotes extracted
T3224 1056-1060 IN denotes from
T3225 1061-1069 VBN denotes depicted
T3226 1070-1074 NN denotes cell
T3227 1075-1080 NNS denotes lines
T3228 1081-1085 IN denotes with
T3229 1086-1087 DT denotes a
T3230 1088-1098 JJ denotes commercial
T3231 1099-1102 NN denotes kit
T3232 1103-1104 -LRB- denotes (
T3233 1104-1110 NNP denotes Qiagen
T3234 1110-1111 , denotes ,
T3235 1112-1117 NNP denotes Hilde
T3236 1117-1118 , denotes ,
T3237 1119-1126 NNP denotes Germany
T3238 1126-1127 -RRB- denotes )
T3239 1128-1130 IN denotes as
T3240 1131-1142 VBN denotes recommended
T3241 1142-1143 . denotes .
T3242 1144-1147 DT denotes The
T3243 1148-1155 NNS denotes primers
T3244 1156-1160 VBD denotes were
T3245 1161-1170 JJ denotes 1-forward
T3246 1170-1171 : denotes :
T3247 1172-1173 CD denotes 5
T3248 1173-1174 SYM denotes ′
T3249 1174-1175 : denotes -
T3250 1175-1199 NN denotes TTGAGATGGAGTCTTGCTCTGT-3
T3251 1199-1200 NN denotes ′
T3252 1200-1201 , denotes ,
T3253 1202-1211 JJ denotes 1-reverse
T3254 1211-1212 : denotes :
T3255 1213-1214 CD denotes 5
T3256 1214-1215 SYM denotes ′
T3257 1215-1216 : denotes -
T3258 1216-1241 NN denotes CCAGGACCTCAGGAGGCCAGTCA-3
T3259 1241-1242 NN denotes ′
T3260 1242-1243 : denotes ;
T3261 1244-1253 JJ denotes 2-forward
T3262 1253-1254 : denotes :
T3263 1255-1256 CD denotes 5
T3264 1256-1257 SYM denotes ′
T3265 1257-1258 : denotes -
T3266 1258-1285 NN denotes AGCGGTGAAACTGAGAGTGCGAGGT-3
T3267 1285-1286 NN denotes ′
T3268 1286-1287 , denotes ,
T3269 1288-1297 JJ denotes 2-reverse
T3270 1297-1298 : denotes :
T3271 1299-1300 CD denotes 5
T3272 1300-1301 SYM denotes ′
T3273 1301-1302 : denotes -
T3274 1302-1324 NN denotes GCCACATCGCTGCAGTTTAG-3
T3275 1324-1325 NN denotes ′
T3276 1325-1326 . denotes .
T3277 1327-1330 DT denotes The
T3278 1331-1339 NNS denotes products
T3279 1340-1344 VBD denotes were
T3280 1345-1351 VBN denotes cloned
T3281 1352-1356 IN denotes with
T3282 1357-1360 DT denotes the
T3283 1362-1366 NNP denotes TOPO
T3284 1367-1369 NNP denotes TA
T3285 1370-1377 VBG denotes cloning
T3286 1378-1381 NN denotes kit
T3287 1383-1384 -LRB- denotes (
T3288 1384-1394 NNP denotes Invitrogen
T3289 1394-1395 , denotes ,
T3290 1396-1405 NNP denotes Groningen
T3291 1405-1406 , denotes ,
T3292 1407-1410 DT denotes The
T3293 1411-1422 NNP denotes Netherlands
T3294 1422-1423 -RRB- denotes )
T3295 1423-1424 . denotes .
T3296 1425-1430 IN denotes After
T3297 1431-1440 JJ denotes bacterial
T3298 1441-1454 NN denotes amplification
T3299 1455-1457 IN denotes of
T3300 1458-1461 DT denotes the
T3301 1462-1468 VBN denotes cloned
T3302 1469-1472 NNP denotes PCR
T3303 1473-1482 NNS denotes fragments
T3304 1483-1485 IN denotes by
T3305 1486-1494 JJ denotes standard
T3306 1495-1505 NNS denotes procedures
T3307 1505-1506 , denotes ,
T3308 1507-1509 IN denotes at
T3309 1510-1515 JJS denotes least
T3310 1516-1521 CD denotes three
T3311 1522-1528 NNS denotes clones
T3312 1529-1533 IN denotes from
T3313 1534-1538 DT denotes each
T3314 1539-1545 NN denotes sample
T3315 1546-1550 VBD denotes were
T3316 1551-1560 VBN denotes sequenced
T3317 1561-1565 IN denotes with
T3318 1566-1568 DT denotes an
T3319 1569-1578 VBN denotes automated
T3320 1579-1588 NN denotes sequencer
T3321 1589-1590 -LRB- denotes (
T3322 1590-1593 NNP denotes ABI
T3323 1594-1599 NNP denotes Prism
T3324 1600-1603 CD denotes 377
T3325 1603-1604 , denotes ,
T3326 1605-1612 NNP denotes Applied
T3327 1613-1624 NNPS denotes Bio-systems
T3328 1624-1625 , denotes ,
T3329 1626-1632 NNP denotes Foster
T3330 1633-1637 NNP denotes City
T3331 1637-1638 , denotes ,
T3332 1639-1642 NNP denotes USA
T3333 1642-1643 -RRB- denotes )
T3334 1644-1646 IN denotes as
T3335 1647-1658 VBN denotes recommended
T3336 1659-1661 IN denotes by
T3337 1662-1665 DT denotes the
T3338 1666-1678 NN denotes manufacturer
T3339 1678-1679 . denotes .
T3802 1681-1691 NN denotes Expression
T3803 1692-1700 NN denotes analysis
T3804 1701-1703 TO denotes To
T3805 1704-1711 VB denotes analyze
T3806 1712-1715 DT denotes the
T3807 1716-1721 NN denotes IRF-4
T3808 1722-1737 JJ denotes transcriptional
T3809 1738-1743 NN denotes level
T3810 1743-1744 , denotes ,
T3811 1745-1748 NNP denotes RNA
T3812 1749-1752 VBD denotes was
T3813 1753-1762 VBN denotes extracted
T3814 1763-1767 IN denotes from
T3815 1768-1773 NNS denotes cells
T3816 1774-1779 VBG denotes using
T3817 1780-1783 DT denotes the
T3818 1784-1794 JJ denotes commercial
T3819 1795-1805 NN denotes RNAzol-kit
T3820 1806-1807 -LRB- denotes (
T3821 1807-1813 NNP denotes Paesel
T3822 1813-1814 , denotes ,
T3823 1815-1824 NNP denotes Frankfurt
T3824 1824-1825 , denotes ,
T3825 1826-1833 NNP denotes Germany
T3826 1833-1834 -RRB- denotes )
T3827 1834-1835 . denotes .
T3828 1836-1838 DT denotes An
T3829 1839-1846 NN denotes aliquot
T3830 1847-1849 IN denotes of
T3831 1850-1851 CD denotes 1
T3832 1852-1854 NN denotes μg
T3833 1855-1860 JJ denotes total
T3834 1861-1864 NNP denotes RNA
T3835 1865-1868 VBD denotes was
T3836 1869-1873 VBN denotes used
T3837 1874-1877 IN denotes for
T3838 1878-1882 NNP denotes cDNA
T3839 1883-1892 NN denotes synthesis
T3840 1893-1895 IN denotes as
T3841 1896-1905 VBN denotes described
T3842 1906-1916 RB denotes previously
T3843 1917-1918 -LRB- denotes (
T3844 1918-1920 CD denotes 27
T3845 1920-1921 -RRB- denotes )
T3846 1921-1922 . denotes .
T3847 1923-1926 NNP denotes RNA
T3848 1927-1937 NN denotes expression
T3849 1938-1946 NN denotes analysis
T3850 1947-1950 IN denotes for
T3851 1951-1956 NNP denotes IRF-4
T3852 1957-1960 CC denotes and
T3853 1961-1964 DT denotes the
T3854 1965-1974 NN denotes reference
T3855 1975-1979 NN denotes gene
T3856 1980-1987 NN denotes β-actin
T3857 1988-1991 VBD denotes was
T3858 1992-1999 VBN denotes carried
T3859 2000-2003 RP denotes out
T3860 2004-2006 IN denotes by
T3861 2007-2024 JJ denotes semi-quantitative
T3862 2025-2028 NNP denotes PCR
T3863 2029-2031 IN denotes as
T3864 2032-2041 VBN denotes described
T3865 2042-2052 RB denotes previously
T3866 2053-2054 -LRB- denotes (
T3867 2054-2058 CD denotes 3,27
T3868 2058-2059 -RRB- denotes )
T3869 2059-2060 . denotes .
T3870 2061-2064 NNP denotes PCR
T3871 2065-2073 NNS denotes products
T3872 2074-2078 VBD denotes were
T3873 2079-2087 VBN denotes verified
T3874 2088-2090 IN denotes by
T3875 2091-2100 JJ denotes automated
T3876 2101-2111 NN denotes sequencing
T3877 2111-2112 . denotes .
T3878 2113-2116 NNP denotes PCR
T3879 2117-2124 NNS denotes primers
T3880 2125-2128 CC denotes and
T3881 2129-2139 NNS denotes conditions
T3882 2140-2143 IN denotes for
T3883 2144-2154 NN denotes expression
T3884 2155-2163 NN denotes analysis
T3885 2164-2166 IN denotes of
T3886 2167-2171 NNP denotes DNMT
T3887 2172-2174 CC denotes or
T3888 2175-2178 NNP denotes MBP
T3889 2179-2180 -LRB- denotes (
T3890 2180-2185 NNP denotes DNMT1
T3891 2186-2192 NNP denotes DNMT3A
T3892 2192-2193 , denotes ,
T3893 2194-2200 NNP denotes DNMT3B
T3894 2200-2201 , denotes ,
T3895 2202-2206 NNP denotes MeCP
T3896 2206-2207 , denotes ,
T3897 2208-2212 NNP denotes MBD1
T3898 2212-2213 , denotes ,
T3899 2214-2218 NNP denotes MBD2
T3900 2219-2222 CC denotes and
T3901 2223-2227 NNP denotes MBD4
T3902 2227-2228 -RRB- denotes )
T3903 2229-2233 VBD denotes were
T3904 2234-2243 VBN denotes published
T3905 2244-2253 RB denotes elsewhere
T3906 2254-2255 -LRB- denotes (
T3907 2255-2257 CD denotes 28
T3908 2257-2258 -RRB- denotes )
T3909 2258-2259 . denotes .
T3910 2260-2263 IN denotes For
T3911 2264-2272 NN denotes analysis
T3912 2273-2275 IN denotes of
T3913 2276-2281 NN denotes IRF-4
T3914 2282-2289 NN denotes protein
T3915 2290-2300 NN denotes expression
T3916 2300-2301 , denotes ,
T3917 2302-2303 DT denotes a
T3918 2304-2312 JJ denotes standard
T3919 2313-2327 NN denotes immunoblotting
T3920 2328-2333 NN denotes assay
T3921 2334-2337 VBD denotes was
T3922 2338-2347 VBN denotes performed
T3923 2348-2350 IN denotes as
T3924 2351-2360 VBN denotes described
T3925 2361-2371 RB denotes previously
T3926 2372-2373 -LRB- denotes (
T3927 2373-2375 CD denotes 29
T3928 2375-2376 -RRB- denotes )
T3929 2376-2377 . denotes .
T3930 2378-2385 RB denotes Briefly
T3931 2385-2386 , denotes ,
T3932 2387-2394 NN denotes protein
T3933 2395-2402 NNS denotes lysates
T3934 2403-2407 VBD denotes were
T3935 2408-2417 VBN denotes generated
T3936 2418-2420 IN denotes by
T3937 2421-2431 VBG denotes incubating
T3938 2432-2433 CD denotes 1
T3939 2434-2435 CD denotes ×
T3940 2436-2439 CD denotes 106
T3941 2440-2445 NNS denotes cells
T3942 2446-2448 IN denotes in
T3943 2449-2452 CD denotes 100
T3944 2453-2455 NN denotes µl
T3945 2456-2460 NNP denotes RIPA
T3946 2461-2467 NN denotes buffer
T3947 2468-2469 -LRB- denotes (
T3948 2469-2470 CD denotes 1
T3949 2470-2471 NN denotes %
T3950 2472-2477 NN denotes NP-40
T3951 2477-2478 , denotes ,
T3952 2479-2482 CD denotes 0.5
T3953 2482-2483 NN denotes %
T3954 2484-2503 NN denotes sodiumdesoxycholate
T3955 2503-2504 , denotes ,
T3956 2505-2508 CD denotes 0.1
T3957 2508-2509 NN denotes %
T3958 2510-2513 NNP denotes SDS
T3959 2513-2514 , denotes ,
T3960 2515-2518 CD denotes 100
T3961 2519-2521 NN denotes µg
T3962 2521-2522 NN denotes /
T3963 2522-2524 NN denotes ml
T3964 2525-2545 NN denotes phenylmethylsulfonyl
T3965 2546-2554 NN denotes fluoride
T3966 2554-2555 , denotes ,
T3967 2556-2558 CD denotes 10
T3968 2559-2561 NN denotes µl
T3969 2561-2562 NN denotes /
T3970 2562-2564 NN denotes ml
T3971 2565-2588 NN denotes protease-inhibitory-mix
T3972 2588-2589 , denotes ,
T3973 2590-2591 CD denotes 1
T3974 2592-2596 NN denotes µmol
T3975 2596-2597 CD denotes /
T3976 2597-2599 NN denotes ml
T3977 2600-2619 NN denotes sodiumorthovanadate
T3978 2620-2622 IN denotes in
T3979 2623-2641 JJ denotes phosphate-buffered
T3980 2642-2648 NN denotes saline
T3981 2648-2649 -RRB- denotes )
T3982 2650-2653 IN denotes for
T3983 2654-2656 CD denotes 30
T3984 2657-2660 NN denotes min
T3985 2661-2663 IN denotes on
T3986 2664-2667 NN denotes ice
T3987 2667-2668 . denotes .
T3988 2669-2674 IN denotes After
T3989 2675-2689 NN denotes centrifugation
T3990 2689-2690 , denotes ,
T3991 2691-2698 NN denotes protein
T3992 2699-2712 NN denotes concentration
T3993 2713-2715 IN denotes of
T3994 2716-2719 DT denotes the
T3995 2720-2731 NN denotes supernatant
T3996 2732-2735 VBD denotes was
T3997 2736-2746 VBN denotes determined
T3998 2747-2749 IN denotes by
T3999 2750-2760 JJ denotes BCA-method
T4000 2761-2762 -LRB- denotes (
T4001 2762-2768 NNP denotes Pierce
T4002 2768-2769 , denotes ,
T4003 2770-2778 NNP denotes Rockford
T4004 2778-2779 , denotes ,
T4005 2780-2782 NNP denotes IL
T4006 2782-2783 -RRB- denotes )
T4007 2784-2786 IN denotes as
T4008 2787-2798 VBN denotes recommended
T4009 2798-2799 . denotes .
T4010 2800-2807 NNP denotes Protein
T4011 2808-2815 VBZ denotes lysates
T4012 2816-2817 -LRB- denotes (
T4013 2817-2819 CD denotes 70
T4014 2820-2823 CD denotes 100
T4015 2824-2826 NN denotes µg
T4016 2826-2827 -RRB- denotes )
T4017 2828-2832 VBD denotes were
T4018 2833-2848 VBN denotes electrophoresed
T4019 2849-2851 IN denotes on
T4020 2852-2866 NN denotes polyacrylamide
T4021 2867-2871 NNS denotes gels
T4022 2872-2875 CC denotes and
T4023 2876-2887 VBN denotes transferred
T4024 2888-2890 TO denotes to
T4025 2891-2892 DT denotes a
T4026 2893-2906 NN denotes PVDF-membrane
T4027 2907-2908 -LRB- denotes (
T4028 2908-2917 NNP denotes Immobilon
T4029 2918-2919 NNP denotes P
T4030 2919-2920 , denotes ,
T4031 2921-2925 CD denotes 0.45
T4032 2926-2928 NN denotes µm
T4033 2928-2929 : denotes ;
T4034 2930-2939 NNP denotes Millipore
T4035 2939-2940 , denotes ,
T4036 2941-2949 NNP denotes Eschborn
T4037 2949-2950 , denotes ,
T4038 2951-2958 NNP denotes Germany
T4039 2958-2959 -RRB- denotes )
T4040 2959-2960 . denotes .
T4041 2961-2970 NNS denotes Membranes
T4042 2971-2975 VBD denotes were
T4043 2976-2983 VBN denotes blocked
T4044 2984-2988 IN denotes with
T4045 2989-2992 CD denotes 2.5
T4046 2992-2993 NN denotes %
T4047 2994-3002 VBG denotes blocking
T4048 3003-3010 NN denotes reagent
T4049 3011-3012 -LRB- denotes (
T4050 3012-3022 NNP denotes Boehringer
T4051 3023-3031 NNP denotes Mannheim
T4052 3031-3032 , denotes ,
T4053 3033-3040 NNP denotes Germany
T4054 3040-3041 -RRB- denotes )
T4055 3042-3044 IN denotes in
T4056 3045-3049 NNP denotes TBST
T4057 3050-3056 NN denotes buffer
T4058 3057-3058 -LRB- denotes (
T4059 3058-3062 CD denotes 4.44
T4060 3063-3066 NN denotes g/l
T4061 3067-3071 NNP denotes Tris
T4062 3072-3075 NNP denotes HCL
T4063 3075-3076 , denotes ,
T4064 3077-3081 CD denotes 2.65
T4065 3082-3085 NN denotes g/l
T4066 3086-3092 NNP denotes TrisOH
T4067 3092-3093 , denotes ,
T4068 3094-3098 CD denotes 8.07
T4069 3099-3102 NN denotes g/l
T4070 3103-3107 NNP denotes NaCl
T4071 3107-3108 , denotes ,
T4072 3109-3112 CD denotes 0.2
T4073 3113-3116 NN denotes g/l
T4074 3117-3120 NNP denotes KCl
T4075 3121-3124 CC denotes and
T4076 3125-3128 CD denotes 500
T4077 3129-3131 NN denotes µl
T4078 3131-3132 NN denotes /
T4079 3132-3133 NN denotes l
T4080 3134-3142 NN denotes Tween-20
T4081 3143-3145 IN denotes in
T4082 3146-3149 CD denotes H2O
T4083 3149-3150 -RRB- denotes )
T4084 3151-3154 CC denotes and
T4085 3155-3167 RB denotes subsequently
T4086 3168-3177 VBN denotes incubated
T4087 3178-3182 IN denotes with
T4088 3183-3190 JJ denotes primary
T4089 3191-3199 NN denotes antibody
T4090 3200-3202 IN denotes as
T4091 3203-3212 VBN denotes indicated
T4092 3213-3216 CC denotes and
T4093 3217-3228 JJ denotes horseradish
T4094 3229-3250 JJ denotes peroxidase-conjugated
T4095 3251-3260 JJ denotes secondary
T4096 3261-3269 NN denotes antibody
T4097 3269-3270 , denotes ,
T4098 3271-3281 JJ denotes anti-mouse
T4099 3282-3284 CC denotes or
T4100 3285-3294 JJ denotes anti-goat
T4101 3295-3298 NNP denotes IgG
T4102 3299-3300 -LRB- denotes (
T4103 3300-3304 NNP denotes DAKO
T4104 3304-3305 , denotes ,
T4105 3306-3313 NNP denotes Hamburg
T4106 3313-3314 , denotes ,
T4107 3315-3322 NNP denotes Germany
T4108 3322-3323 -RRB- denotes )
T4109 3323-3324 , denotes ,
T4110 3325-3337 RB denotes respectively
T4111 3337-3338 . denotes .
T4112 3339-3342 DT denotes The
T4113 3343-3352 NNS denotes membranes
T4114 3353-3357 VBD denotes were
T4115 3358-3362 RB denotes then
T4116 3363-3372 VBN denotes developed
T4117 3373-3377 IN denotes with
T4118 3378-3380 DT denotes an
T4119 3381-3384 NNP denotes ECL
T4120 3385-3394 NN denotes detection
T4121 3395-3398 NN denotes kit
T4122 3399-3400 -LRB- denotes (
T4123 3400-3408 NNP denotes Amersham
T4124 3409-3418 NNP denotes Pharmacia
T4125 3419-3426 NNP denotes Biotech
T4126 3426-3427 , denotes ,
T4127 3428-3436 NNP denotes Freiburg
T4128 3436-3437 , denotes ,
T4129 3438-3445 NNP denotes Germany
T4130 3445-3446 -RRB- denotes )
T4131 3446-3447 . denotes .
T4132 3448-3451 DT denotes The
T4133 3452-3459 JJ denotes primary
T4134 3460-3470 NNS denotes antibodies
T4135 3471-3475 VBD denotes were
T4136 3476-3480 JJ denotes goat
T4137 3481-3491 JJ denotes anti-IRF-4
T4138 3491-3492 NN denotes /
T4139 3492-3497 NNP denotes ICSAT
T4140 3498-3499 -LRB- denotes (
T4141 3499-3503 NNP denotes M-17
T4142 3503-3504 -RRB- denotes )
T4143 3505-3506 -LRB- denotes (
T4144 3506-3511 NNP denotes Santa
T4145 3512-3516 NNP denotes Cruz
T4146 3517-3530 NNP denotes Biotechnology
T4147 3530-3531 , denotes ,
T4148 3532-3537 NNP denotes Santa
T4149 3538-3542 NNP denotes Cruz
T4150 3542-3543 , denotes ,
T4151 3544-3546 NNP denotes CA
T4152 3546-3547 -RRB- denotes )
T4153 3548-3551 CC denotes and
T4154 3552-3557 NN denotes mouse
T4155 3558-3570 NN denotes anti-β-actin
T4156 3571-3572 -LRB- denotes (
T4157 3572-3577 NN denotes AC-74
T4158 3577-3578 -RRB- denotes )
T4159 3579-3580 -LRB- denotes (
T4160 3580-3585 NNP denotes Sigma
T4161 3585-3586 -RRB- denotes )
T4162 3586-3587 . denotes .
T4626 3589-3609 JJ denotes Methylation-specific
T4627 3610-3631 JJ denotes restriction-PCR-assay
T4628 3632-3635 NN denotes DNA
T4629 3636-3639 VBD denotes was
T4630 3640-3649 VBN denotes extracted
T4631 3650-3654 IN denotes with
T4632 3655-3656 DT denotes a
T4633 3657-3667 JJ denotes commercial
T4634 3668-3671 NN denotes kit
T4635 3672-3673 -LRB- denotes (
T4636 3673-3679 NNP denotes Qiagen
T4637 3679-3680 -RRB- denotes )
T4638 3681-3683 IN denotes as
T4639 3684-3695 VBN denotes recommended
T4640 3695-3696 . denotes .
T4641 3697-3702 IN denotes Since
T4642 3703-3706 DT denotes the
T4643 3707-3718 NN denotes restriction
T4644 3719-3726 NN denotes ability
T4645 3727-3729 IN denotes of
T4646 3730-3737 JJ denotes several
T4647 3738-3751 NNS denotes endonucleases
T4648 3752-3754 VBZ denotes is
T4649 3755-3764 VBN denotes inhibited
T4650 3765-3767 IN denotes by
T4651 3768-3779 NN denotes methylation
T4652 3780-3782 IN denotes of
T4653 3783-3788 PRP$ denotes their
T4654 3789-3795 NN denotes target
T4655 3796-3804 NN denotes sequence
T4656 3804-3805 , denotes ,
T4657 3806-3808 PRP denotes we
T4658 3809-3813 VBD denotes used
T4659 3814-3835 JJ denotes methylation-sensitive
T4660 3836-3843 NNS denotes enzymes
T4661 3844-3849 NNP denotes HpaII
T4662 3850-3853 CC denotes and
T4663 3854-3871 NNP denotes HaeII-isochizomer
T4664 3872-3880 NNP denotes Bsp143II
T4665 3881-3884 CC denotes and
T4666 3885-3893 NNP denotes Bsh1236I
T4667 3894-3895 -LRB- denotes (
T4668 3895-3898 NNP denotes MBI
T4669 3899-3908 NNP denotes Fermentas
T4670 3908-3909 , denotes ,
T4671 3910-3912 NNP denotes St
T4672 3913-3921 NNP denotes Leon-Rot
T4673 3921-3922 , denotes ,
T4674 3923-3930 NNP denotes Germany
T4675 3930-3931 -RRB- denotes )
T4676 3932-3933 -LRB- denotes (
T4677 3933-3938 CD denotes 20,24
T4678 3938-3939 -RRB- denotes )
T4679 3939-3940 . denotes .
T4680 3941-3943 IN denotes As
T4681 3944-3951 NN denotes control
T4682 3952-3955 DT denotes the
T4683 3956-3977 JJ denotes methylation-resistant
T4684 3978-3984 NN denotes enzyme
T4685 3985-3989 NNP denotes MspI
T4686 3990-3993 CC denotes and
T4687 3994-3996 DT denotes an
T4688 3997-4003 NN denotes enzyme
T4689 4004-4008 IN denotes with
T4690 4009-4011 DT denotes no
T4691 4012-4023 NN denotes recognition
T4692 4024-4028 NN denotes site
T4693 4029-4031 IN denotes in
T4694 4032-4035 DT denotes the
T4695 4036-4042 NN denotes target
T4696 4043-4051 NN denotes promoter
T4697 4051-4052 , denotes ,
T4698 4053-4058 NNP denotes EcoRI
T4699 4058-4059 , denotes ,
T4700 4060-4064 VBD denotes were
T4701 4065-4069 VBN denotes used
T4702 4069-4070 . denotes .
T4703 4071-4074 NNP denotes DNA
T4704 4075-4076 -LRB- denotes (
T4705 4076-4079 CD denotes 0.8
T4706 4080-4082 NN denotes µg
T4707 4082-4083 -RRB- denotes )
T4708 4084-4087 VBD denotes was
T4709 4088-4096 VBN denotes digested
T4710 4097-4099 IN denotes by
T4711 4100-4102 CD denotes 40
T4712 4103-4104 NNP denotes U
T4713 4105-4108 DT denotes the
T4714 4109-4119 JJ denotes respective
T4715 4120-4126 NN denotes enzyme
T4716 4127-4130 IN denotes for
T4717 4131-4132 CD denotes 6
T4718 4133-4134 NN denotes h
T4719 4135-4138 CC denotes and
T4720 4138-4139 , denotes ,
T4721 4140-4142 TO denotes to
T4722 4143-4149 VB denotes ensure
T4723 4150-4158 JJ denotes complete
T4724 4159-4167 NN denotes cleavage
T4725 4167-4168 , denotes ,
T4726 4169-4179 JJ denotes additional
T4727 4180-4182 CD denotes 20
T4728 4183-4184 NNP denotes U
T4729 4185-4188 IN denotes for
T4730 4189-4191 CD denotes 16
T4731 4192-4194 NN denotes h.
T4732 4195-4205 RB denotes Thereafter
T4733 4206-4209 CD denotes 100
T4734 4210-4212 NN denotes ng
T4735 4213-4215 IN denotes of
T4736 4216-4224 VBN denotes digested
T4737 4225-4228 NN denotes DNA
T4738 4229-4232 VBD denotes was
T4739 4233-4237 VBN denotes used
T4740 4238-4240 TO denotes to
T4741 4241-4242 DT denotes a
T4742 4243-4246 NNP denotes PCR
T4743 4247-4260 NN denotes amplification
T4744 4261-4263 IN denotes of
T4745 4264-4267 CD denotes two
T4746 4268-4277 NNS denotes fragments
T4747 4278-4279 -LRB- denotes (
T4748 4279-4281 CD denotes F1
T4749 4282-4285 CC denotes and
T4750 4286-4288 CD denotes F2
T4751 4288-4289 -RRB- denotes )
T4752 4290-4298 VBG denotes spanning
T4753 4299-4303 NN denotes part
T4754 4304-4306 IN denotes of
T4755 4307-4310 DT denotes the
T4756 4311-4316 NN denotes IRF-4
T4757 4317-4325 NN denotes promoter
T4758 4326-4327 -LRB- denotes (
T4759 4327-4329 CD denotes 30
T4760 4329-4330 -RRB- denotes )
T4761 4331-4332 -LRB- denotes (
T4762 4332-4339 NNP denotes GenBank
T4763 4340-4346 NNP denotes U52683
T4764 4346-4347 : denotes ;
T4765 4348-4351 VB denotes see
T4766 4352-4358 NN denotes Figure
T4767 4359-4361 NN denotes 3A
T4768 4361-4362 -RRB- denotes )
T4769 4362-4363 . denotes .
T4770 4364-4367 DT denotes The
T4771 4368-4377 NNS denotes sequences
T4772 4378-4380 IN denotes of
T4773 4381-4384 DT denotes the
T4774 4385-4392 NNS denotes primers
T4775 4393-4397 VBD denotes were
T4776 4398-4408 JJ denotes F1-forward
T4777 4408-4409 : denotes :
T4778 4410-4411 CD denotes 5
T4779 4411-4412 SYM denotes ′
T4780 4412-4413 : denotes -
T4781 4413-4437 NN denotes TTGAGATGGAGTCTTGCTCTGT-3
T4782 4437-4438 NN denotes ′
T4783 4438-4439 , denotes ,
T4784 4440-4450 NN denotes F1-reverse
T4785 4450-4451 : denotes :
T4786 4452-4479 JJ denotes ATCACTTCCAGACTTCAGTTCACCT-3
T4787 4479-4480 NN denotes ′
T4788 4481-4482 -LRB- denotes (
T4789 4482-4485 CD denotes 341
T4790 4486-4488 NN denotes bp
T4791 4488-4489 -RRB- denotes )
T4792 4489-4490 : denotes ;
T4793 4491-4501 NNP denotes F2-forward
T4794 4501-4502 : denotes :
T4795 4503-4504 CD denotes 5
T4796 4504-4505 SYM denotes ′
T4797 4505-4506 : denotes -
T4798 4506-4533 NN denotes AAGGTGAACTGAAGTCTGGAAGTGA-3
T4799 4533-4534 NN denotes ′
T4800 4534-4535 , denotes ,
T4801 4536-4546 NN denotes F2-reverse
T4802 4546-4547 : denotes :
T4803 4548-4549 CD denotes 5
T4804 4549-4550 SYM denotes ′
T4805 4550-4551 : denotes -
T4806 4551-4576 NN denotes CCAGGACCTCAGGAGGCCAGTCA-3
T4807 4576-4577 NN denotes ′
T4808 4578-4579 -LRB- denotes (
T4809 4579-4582 CD denotes 474
T4810 4583-4585 NN denotes bp
T4811 4585-4586 -RRB- denotes )
T4812 4586-4587 . denotes .
T4813 4588-4591 DT denotes The
T4814 4592-4595 NNP denotes PCR
T4815 4596-4606 NNS denotes conditions
T4816 4607-4611 VBD denotes were
T4817 4612-4621 VBN denotes described
T4818 4622-4631 RB denotes elsewhere
T4819 4632-4633 -LRB- denotes (
T4820 4633-4634 LS denotes 3
T4821 4634-4635 -RRB- denotes )
T4822 4635-4636 . denotes .
T4823 4637-4640 NNP denotes PCR
T4824 4641-4644 VBD denotes was
T4825 4645-4654 VBN denotes performed
T4826 4655-4659 IN denotes with
T4827 4660-4662 DT denotes an
T4828 4663-4672 NN denotes annealing
T4829 4673-4684 NN denotes temperature
T4830 4685-4687 IN denotes of
T4831 4688-4690 CD denotes 62
T4832 4690-4691 CD denotes °
T4833 4691-4692 NNP denotes C
T4834 4693-4696 CC denotes and
T4835 4697-4699 CD denotes 35
T4836 4700-4706 NNS denotes cycles
T4837 4706-4707 . denotes .
T4838 4708-4712 WRB denotes When
T4839 4713-4716 NNP denotes DNA
T4840 4717-4720 VBD denotes was
T4841 4721-4731 VBN denotes methylated
T4842 4732-4734 IN denotes at
T4843 4735-4743 JJ denotes specific
T4844 4744-4749 NNS denotes sites
T4845 4749-4750 , denotes ,
T4846 4751-4754 DT denotes the
T4847 4755-4764 JJ denotes sensitive
T4848 4765-4772 NNS denotes enzymes
T4849 4773-4777 VBD denotes were
T4850 4778-4781 RB denotes not
T4851 4782-4786 JJ denotes able
T4852 4787-4789 TO denotes to
T4853 4790-4796 VB denotes digest
T4854 4797-4800 DT denotes the
T4855 4801-4804 NN denotes DNA
T4856 4805-4808 CC denotes and
T4857 4809-4822 NN denotes amplification
T4858 4823-4827 VBD denotes took
T4859 4828-4833 NN denotes place
T4860 4833-4834 : denotes ;
T4861 4835-4837 IN denotes in
T4862 4838-4842 NN denotes case
T4863 4843-4845 IN denotes of
T4864 4846-4848 DT denotes no
T4865 4849-4860 NN denotes methylation
T4866 4860-4861 , denotes ,
T4867 4862-4865 NNP denotes DNA
T4868 4866-4869 VBD denotes was
T4869 4870-4878 VBN denotes digested
T4870 4879-4882 CC denotes and
T4871 4883-4885 DT denotes no
T4872 4886-4893 NN denotes product
T4873 4894-4897 VBD denotes was
T4874 4898-4907 VBN denotes generated
T4875 4907-4908 . denotes .
T4876 4909-4912 DT denotes The
T4877 4913-4916 NNP denotes PCR
T4878 4917-4925 NNS denotes products
T4879 4926-4930 VBD denotes were
T4880 4931-4946 VBN denotes electrophoresed
T4881 4947-4949 IN denotes on
T4882 4950-4951 DT denotes a
T4883 4952-4953 CD denotes 3
T4884 4953-4954 NN denotes %
T4885 4955-4962 JJ denotes agarose
T4886 4963-4966 NN denotes gel
T4887 4966-4967 , denotes ,
T4888 4968-4972 VBD denotes were
T4889 4973-4980 VBN denotes stained
T4890 4981-4985 IN denotes with
T4891 4986-4994 NN denotes ethidium
T4892 4995-5002 NN denotes bromide
T4893 5003-5006 CC denotes and
T4894 5007-5019 VBD denotes photographed
T4895 5019-5020 . denotes .
T4896 5021-5024 NNP denotes PCR
T4897 5025-5033 NNS denotes products
T4898 5034-5038 VBD denotes were
T4899 5039-5047 VBN denotes verified
T4900 5048-5050 IN denotes by
T4901 5051-5060 JJ denotes automated
T4902 5061-5071 NN denotes sequencing
T4903 5071-5072 . denotes .
T5272 5074-5083 NNP denotes Bisulfite
T5273 5084-5093 NN denotes treatment
T5274 5094-5097 NN denotes DNA
T5275 5098-5101 VBD denotes was
T5276 5102-5111 VBN denotes extracted
T5277 5112-5114 IN denotes as
T5278 5115-5124 VBN denotes described
T5279 5125-5130 IN denotes above
T5280 5130-5131 . denotes .
T5281 5132-5141 NNP denotes Bisulfite
T5282 5142-5151 NN denotes treatment
T5283 5152-5154 IN denotes of
T5284 5155-5158 NNP denotes DNA
T5285 5158-5159 , denotes ,
T5286 5160-5167 VBG denotes leading
T5287 5168-5170 TO denotes to
T5288 5171-5181 NN denotes conversion
T5289 5182-5184 IN denotes of
T5290 5185-5197 JJ denotes unmethylated
T5291 5198-5206 NN denotes cytosine
T5292 5207-5209 TO denotes to
T5293 5210-5216 VB denotes uracil
T5294 5217-5225 NNS denotes residues
T5295 5226-5229 CC denotes and
T5296 5230-5232 DT denotes no
T5297 5233-5239 NN denotes change
T5298 5240-5242 IN denotes of
T5299 5243-5253 JJ denotes methylated
T5300 5254-5262 NN denotes cytosine
T5301 5263-5271 NNS denotes residues
T5302 5271-5272 , denotes ,
T5303 5273-5276 VBD denotes was
T5304 5277-5286 VBN denotes performed
T5305 5287-5289 IN denotes as
T5306 5290-5299 VBN denotes described
T5307 5300-5302 IN denotes as
T5308 5303-5310 VBZ denotes follows
T5309 5310-5311 . denotes .
T5310 5312-5319 RB denotes Briefly
T5311 5319-5320 , denotes ,
T5312 5321-5322 CD denotes 1
T5313 5323-5325 NN denotes µg
T5314 5326-5328 IN denotes of
T5315 5329-5332 NNP denotes DNA
T5316 5333-5336 CC denotes and
T5317 5337-5338 CD denotes 2
T5318 5339-5341 NN denotes µg
T5319 5342-5344 IN denotes of
T5320 5345-5349 NN denotes poly
T5321 5349-5350 -LRB- denotes (
T5322 5350-5352 NN denotes dA
T5323 5353-5355 NN denotes dT
T5324 5355-5356 -RRB- denotes )
T5325 5356-5357 -LRB- denotes (
T5326 5357-5361 NN denotes poly
T5327 5361-5362 -LRB- denotes (
T5328 5362-5364 NN denotes dA
T5329 5365-5367 NN denotes dT
T5330 5367-5368 -RRB- denotes )
T5331 5369-5379 NNS denotes copolymers
T5332 5380-5381 -LRB- denotes (
T5333 5381-5389 NNP denotes Amersham
T5334 5390-5399 NNP denotes Pharmacia
T5335 5400-5407 NNP denotes Biotech
T5336 5407-5408 -RRB- denotes )
T5337 5409-5413 VBD denotes were
T5338 5414-5425 VBN denotes denaturated
T5339 5426-5429 IN denotes for
T5340 5430-5432 CD denotes 20
T5341 5433-5436 NN denotes min
T5342 5437-5439 IN denotes at
T5343 5440-5442 CD denotes 42
T5344 5442-5443 CD denotes °
T5345 5443-5444 NNP denotes C
T5346 5445-5447 IN denotes in
T5347 5448-5451 CD denotes 0.3
T5348 5452-5453 NNP denotes M
T5349 5454-5458 NNP denotes NaOH
T5350 5459-5461 IN denotes in
T5351 5462-5463 DT denotes a
T5352 5464-5470 NN denotes volume
T5353 5471-5473 IN denotes of
T5354 5474-5476 CD denotes 50
T5355 5477-5479 NN denotes µl
T5356 5479-5480 . denotes .
T5357 5481-5486 JJ denotes Fresh
T5358 5487-5496 NNS denotes solutions
T5359 5497-5499 IN denotes of
T5360 5500-5502 CD denotes 30
T5361 5503-5505 NN denotes µl
T5362 5506-5508 IN denotes of
T5363 5509-5511 CD denotes 10
T5364 5512-5514 NN denotes mM
T5365 5515-5526 NN denotes hydrochinon
T5366 5527-5528 -LRB- denotes (
T5367 5528-5533 NNP denotes Sigma
T5368 5533-5534 -RRB- denotes )
T5369 5535-5538 CC denotes and
T5370 5539-5542 CD denotes 530
T5371 5543-5545 NN denotes µl
T5372 5546-5548 IN denotes of
T5373 5549-5550 CD denotes 3
T5374 5551-5552 NNP denotes M
T5375 5553-5559 NN denotes sodium
T5376 5560-5569 NN denotes bisulfite
T5377 5570-5571 -LRB- denotes (
T5378 5571-5573 NNP denotes pH
T5379 5574-5577 CD denotes 5.0
T5380 5577-5578 : denotes ;
T5381 5579-5584 NNP denotes Sigma
T5382 5584-5585 -RRB- denotes )
T5383 5586-5590 VBD denotes were
T5384 5591-5596 VBN denotes added
T5385 5596-5597 , denotes ,
T5386 5598-5601 DT denotes the
T5387 5602-5610 NN denotes solution
T5388 5611-5614 VBD denotes was
T5389 5615-5621 RB denotes gently
T5390 5622-5627 JJ denotes mixed
T5391 5627-5628 , denotes ,
T5392 5629-5638 VBN denotes overlayed
T5393 5639-5643 IN denotes with
T5394 5644-5651 NN denotes mineral
T5395 5652-5655 NN denotes oil
T5396 5656-5659 CC denotes and
T5397 5660-5669 VBN denotes incubated
T5398 5670-5672 IN denotes in
T5399 5673-5676 DT denotes the
T5400 5677-5681 NN denotes dark
T5401 5682-5685 IN denotes for
T5402 5686-5688 CD denotes 12
T5403 5689-5691 CD denotes 13
T5404 5692-5693 NN denotes h
T5405 5694-5696 IN denotes at
T5406 5697-5699 CD denotes 50
T5407 5699-5700 CD denotes °
T5408 5700-5701 NNP denotes C
T5409 5701-5702 . denotes .
T5410 5703-5706 DT denotes The
T5411 5707-5714 JJ denotes aqueous
T5412 5715-5720 NN denotes phase
T5413 5721-5724 VBD denotes was
T5414 5725-5734 VBN denotes recovered
T5415 5735-5740 VBG denotes using
T5416 5741-5744 DT denotes the
T5417 5746-5752 NNP denotes Wizard
T5418 5753-5756 NNP denotes DNA
T5419 5757-5765 JJ denotes clean-up
T5420 5766-5772 NN denotes system
T5421 5774-5775 -LRB- denotes (
T5422 5775-5782 NNP denotes Promega
T5423 5782-5783 , denotes ,
T5424 5784-5792 NNP denotes Mannheim
T5425 5792-5793 , denotes ,
T5426 5794-5801 NNP denotes Germany
T5427 5801-5802 -RRB- denotes )
T5428 5802-5803 . denotes .
T5429 5804-5807 DT denotes The
T5430 5808-5816 JJ denotes purified
T5431 5817-5820 NN denotes DNA
T5432 5821-5824 VBD denotes was
T5433 5825-5837 RB denotes subsequently
T5434 5838-5843 VBN denotes mixed
T5435 5844-5848 IN denotes with
T5436 5849-5850 CD denotes 1
T5437 5851-5852 NNP denotes M
T5438 5853-5857 NNP denotes NaOH
T5439 5858-5860 TO denotes to
T5440 5861-5862 DT denotes a
T5441 5863-5868 JJ denotes final
T5442 5869-5882 NN denotes concentration
T5443 5883-5885 IN denotes of
T5444 5886-5889 CD denotes 0.3
T5445 5890-5891 NNP denotes M
T5446 5892-5895 CC denotes and
T5447 5896-5905 VBN denotes incubated
T5448 5906-5909 IN denotes for
T5449 5910-5912 CD denotes 20
T5450 5913-5916 NN denotes min
T5451 5917-5919 IN denotes at
T5452 5920-5922 CD denotes 37
T5453 5922-5923 CD denotes °
T5454 5923-5924 NNP denotes C
T5455 5925-5927 TO denotes to
T5456 5928-5934 VB denotes ensure
T5457 5935-5943 JJ denotes complete
T5458 5944-5959 NN denotes desulfonisation
T5459 5959-5960 . denotes .
T5460 5961-5964 NNP denotes DNA
T5461 5965-5968 VBD denotes was
T5462 5969-5976 NN denotes ethanol
T5463 5977-5989 VBN denotes precipitated
T5464 5990-5992 IN denotes in
T5465 5993-5996 DT denotes the
T5466 5997-6005 NN denotes presence
T5467 6006-6008 IN denotes of
T5468 6009-6013 CD denotes 1/10
T5469 6014-6017 NN denotes vol
T5470 6018-6020 IN denotes of
T5471 6021-6022 CD denotes 3
T5472 6023-6024 NNP denotes M
T5473 6025-6031 NN denotes sodium
T5474 6032-6039 NN denotes acetate
T5475 6039-6040 , denotes ,
T5476 6041-6047 VBN denotes washed
T5477 6048-6052 IN denotes with
T5478 6053-6055 CD denotes 70
T5479 6055-6056 NN denotes %
T5480 6057-6064 NN denotes ethanol
T5481 6065-6068 CC denotes and
T5482 6069-6080 VBD denotes resuspended
T5483 6081-6083 IN denotes in
T5484 6084-6086 CD denotes 50
T5485 6087-6089 NN denotes µl
T5486 6090-6093 CD denotes H2O
T5487 6093-6094 . denotes .
T5488 6095-6105 JJ denotes Subsequent
T5489 6106-6109 NNP denotes PCR
T5490 6110-6123 NN denotes amplification
T5491 6124-6126 IN denotes of
T5492 6127-6128 CD denotes 4
T5493 6129-6131 NN denotes µl
T5494 6132-6149 JJ denotes bisulfite-treated
T5495 6150-6153 NN denotes DNA
T5496 6154-6157 VBD denotes was
T5497 6158-6162 VBN denotes used
T5498 6163-6166 IN denotes for
T5499 6167-6174 NN denotes cloning
T5500 6175-6177 IN denotes of
T5501 6178-6181 CD denotes two
T5502 6182-6191 NNS denotes fragments
T5503 6192-6194 IN denotes of
T5504 6195-6198 DT denotes the
T5505 6199-6204 NN denotes IRF-4
T5506 6205-6213 NN denotes promoter
T5507 6214-6215 -LRB- denotes (
T5508 6215-6219 NNP denotes BS-I
T5509 6220-6223 CC denotes and
T5510 6224-6229 NNP denotes BS-II
T5511 6229-6230 -RRB- denotes )
T5512 6231-6235 IN denotes into
T5513 6236-6240 JJ denotes pCR2
T5514 6240-6242 CD denotes .1
T5515 6243-6249 NN denotes vector
T5516 6250-6254 IN denotes with
T5517 6255-6258 DT denotes the
T5518 6260-6264 NNP denotes TOPO
T5519 6265-6267 NNP denotes TA
T5520 6268-6275 VBG denotes cloning
T5521 6276-6279 NN denotes kit
T5522 6281-6282 -LRB- denotes (
T5523 6282-6292 NNP denotes Invitrogen
T5524 6292-6293 -RRB- denotes )
T5525 6294-6295 -LRB- denotes (
T5526 6295-6298 VB denotes see
T5527 6299-6305 NN denotes Figure
T5528 6306-6308 NN denotes 3A
T5529 6308-6309 -RRB- denotes )
T5530 6309-6310 . denotes .
T5531 6311-6314 DT denotes The
T5532 6315-6322 NNS denotes primers
T5533 6323-6327 VBN denotes used
T5534 6328-6331 IN denotes for
T5535 6332-6335 NNP denotes PCR
T5536 6336-6349 NN denotes amplification
T5537 6350-6352 IN denotes of
T5538 6353-6356 DT denotes the
T5539 6357-6361 NNP denotes BS-I
T5540 6362-6365 CC denotes and
T5541 6366-6371 NNP denotes BS-II
T5542 6372-6381 NNS denotes fragments
T5543 6382-6389 VBP denotes contain
T5544 6390-6393 DT denotes the
T5545 6394-6402 JJ denotes putative
T5546 6403-6410 JJ denotes altered
T5547 6411-6419 NN denotes sequence
T5548 6420-6422 IN denotes of
T5549 6423-6426 DT denotes the
T5550 6427-6432 NN denotes sense
T5551 6433-6439 NN denotes strand
T5552 6440-6443 JJ denotes due
T5553 6444-6446 TO denotes to
T5554 6447-6456 VB denotes bisulfite
T5555 6457-6466 NN denotes treatment
T5556 6467-6468 -LRB- denotes (
T5557 6468-6477 JJ denotes converted
T5558 6478-6486 NN denotes cytosine
T5559 6487-6495 NNS denotes residues
T5560 6496-6499 VBP denotes are
T5561 6500-6507 VBN denotes written
T5562 6508-6510 IN denotes in
T5563 6511-6515 JJ denotes bold
T5564 6516-6523 NNS denotes letters
T5565 6523-6524 -RRB- denotes )
T5566 6524-6525 : denotes :
T5567 6526-6538 JJ denotes BS-I-forward
T5568 6539-6540 CD denotes 5
T5569 6540-6541 SYM denotes ′
T5570 6541-6542 : denotes -
T5571 6542-6571 NN denotes TATTTGGATTTTTAGGGAGTTTTTTTT-3
T5572 6571-6572 NN denotes ′
T5573 6572-6573 , denotes ,
T5574 6574-6586 JJ denotes BS-I-reverse
T5575 6587-6588 CD denotes 5
T5576 6588-6589 SYM denotes ′
T5577 6589-6590 : denotes -
T5578 6590-6618 NN denotes ACCCAACTCCCTTAAACTATTAAACT-3
T5579 6618-6619 NN denotes ′
T5580 6620-6621 -LRB- denotes (
T5581 6621-6624 CD denotes 187
T5582 6625-6627 NN denotes bp
T5583 6627-6628 -RRB- denotes )
T5584 6628-6629 : denotes ;
T5585 6630-6643 JJ denotes BS-II-forward
T5586 6644-6645 CD denotes 5
T5587 6645-6646 SYM denotes ′
T5588 6646-6647 : denotes -
T5589 6647-6675 NN denotes AGTTTAATAGTTTAAGGGAGTTGGGT-3
T5590 6675-6676 NN denotes ′
T5591 6676-6677 , denotes ,
T5592 6678-6691 JJ denotes BS-II-reverse
T5593 6692-6693 CD denotes 5
T5594 6693-6694 SYM denotes ′
T5595 6694-6695 : denotes -
T5596 6695-6723 NN denotes CTCACCCTAAACTCAAAACTAAAAAC-3
T5597 6723-6724 NN denotes ′
T5598 6725-6726 -LRB- denotes (
T5599 6726-6729 CD denotes 674
T5600 6730-6732 NN denotes bp
T5601 6732-6733 -RRB- denotes )
T5602 6733-6734 . denotes .
T5603 6735-6740 IN denotes After
T5604 6741-6750 JJ denotes bacterial
T5605 6751-6764 NN denotes amplification
T5606 6765-6767 IN denotes of
T5607 6768-6771 DT denotes the
T5608 6772-6778 VBN denotes cloned
T5609 6779-6782 NNP denotes PCR
T5610 6783-6792 NNS denotes fragments
T5611 6793-6795 IN denotes by
T5612 6796-6804 JJ denotes standard
T5613 6805-6815 NNS denotes procedures
T5614 6815-6816 , denotes ,
T5615 6817-6822 CD denotes eight
T5616 6823-6829 NNS denotes clones
T5617 6830-6834 IN denotes from
T5618 6835-6839 DT denotes each
T5619 6840-6846 NN denotes sample
T5620 6847-6851 VBD denotes were
T5621 6852-6861 VBN denotes sequenced
T5622 6862-6866 IN denotes with
T5623 6867-6869 DT denotes an
T5624 6870-6879 VBN denotes automated
T5625 6880-6889 NN denotes sequencer
T5626 6890-6891 -LRB- denotes (
T5627 6891-6894 NNP denotes ABI
T5628 6895-6900 NNP denotes Prism
T5629 6901-6904 CD denotes 377
T5630 6904-6905 , denotes ,
T5631 6906-6913 NNP denotes Applied
T5632 6914-6924 NNPS denotes Biosystems
T5633 6924-6925 -RRB- denotes )
T5634 6925-6926 . denotes .
T5873 6928-6930 IN denotes In
T5874 6931-6936 NN denotes vitro
T5875 6937-6948 NN denotes methylation
T5876 6949-6952 CC denotes and
T5877 6953-6961 NN denotes reporter
T5878 6962-6966 NN denotes gene
T5879 6967-6973 VBZ denotes assays
T5880 6974-6977 DT denotes The
T5881 6978-6983 JJ denotes IRF-4
T5882 6984-7001 NN denotes promoter-reporter
T5883 7002-7006 NN denotes gene
T5884 7007-7016 VBP denotes construct
T5885 7017-7020 VBD denotes was
T5886 7021-7031 RB denotes generously
T5887 7032-7040 VBN denotes provided
T5888 7041-7043 IN denotes by
T5889 7044-7046 NNP denotes J.
T5890 7047-7054 NNP denotes Hiscott
T5891 7055-7056 -LRB- denotes (
T5892 7056-7058 CD denotes 31
T5893 7058-7059 -RRB- denotes )
T5894 7059-7060 . denotes .
T5895 7061-7071 NNS denotes Constructs
T5896 7072-7076 VBD denotes were
T5897 7077-7087 VBN denotes methylated
T5898 7088-7090 IN denotes in
T5899 7091-7096 NN denotes vitro
T5900 7097-7101 IN denotes with
T5901 7102-7105 NNP denotes CpG
T5902 7106-7115 NNP denotes Methylase
T5903 7116-7117 -LRB- denotes (
T5904 7117-7122 NNP denotes M.Sss
T5905 7123-7124 PRP denotes I
T5906 7124-7125 -RRB- denotes )
T5907 7126-7128 IN denotes as
T5908 7129-7140 VBN denotes recommended
T5909 7141-7143 IN denotes by
T5910 7144-7147 DT denotes the
T5911 7148-7160 NN denotes manufacturer
T5912 7161-7162 -LRB- denotes (
T5913 7162-7164 NNP denotes NE
T5914 7165-7172 NNPS denotes Biolabs
T5915 7172-7173 -RRB- denotes )
T5916 7174-7177 CC denotes and
T5917 7178-7186 JJ denotes complete
T5918 7187-7198 NN denotes methylation
T5919 7199-7202 VBD denotes was
T5920 7203-7210 VBN denotes checked
T5921 7211-7214 IN denotes via
T5922 7215-7226 NN denotes restriction
T5923 7227-7235 NN denotes analysis
T5924 7236-7237 -LRB- denotes (
T5925 7237-7243 NN denotes Figure
T5926 7244-7246 NN denotes 5A
T5927 7246-7247 -RRB- denotes )
T5928 7247-7248 . denotes .
T5929 7249-7257 NN denotes Reporter
T5930 7258-7262 NN denotes gene
T5931 7263-7269 NNS denotes assays
T5932 7270-7275 VBG denotes using
T5933 7276-7279 DT denotes the
T5934 7280-7284 JJ denotes dual
T5935 7285-7295 NN denotes luciferase
T5936 7296-7301 NN denotes assay
T5937 7302-7303 -LRB- denotes (
T5938 7303-7310 NNP denotes Promega
T5939 7310-7311 -RRB- denotes )
T5940 7312-7316 VBD denotes were
T5941 7317-7326 VBN denotes performed
T5942 7327-7334 JJ denotes similar
T5943 7335-7337 TO denotes to
T5944 7338-7346 JJ denotes previous
T5945 7347-7354 NNS denotes reports
T5946 7355-7356 -LRB- denotes (
T5947 7356-7358 CD denotes 29
T5948 7358-7359 -RRB- denotes )
T5949 7359-7360 . denotes .
T5950 7361-7368 RB denotes Briefly
T5951 7368-7369 , denotes ,
T5952 7370-7371 CD denotes 5
T5953 7372-7374 NN denotes nM
T5954 7375-7377 IN denotes of
T5955 7378-7381 DT denotes the
T5956 7382-7390 NN denotes reporter
T5957 7391-7400 VB denotes construct
T5958 7401-7404 CC denotes and
T5959 7405-7408 DT denotes the
T5960 7409-7421 NN denotes transfection
T5961 7422-7429 NN denotes control
T5962 7430-7439 VBP denotes construct
T5963 7440-7450 VBG denotes expressing
T5964 7451-7454 DT denotes the
T5965 7455-7462 NN denotes renilla
T5966 7463-7473 NN denotes luciferase
T5967 7474-7478 NN denotes gene
T5968 7479-7483 VBD denotes were
T5969 7484-7507 JJ denotes transientlyco-expressed
T5970 7508-7511 IN denotes via
T5971 7512-7527 NN denotes electroporation
T5972 7527-7528 . denotes .
T5973 7529-7532 DT denotes The
T5974 7533-7540 NN denotes control
T5975 7541-7550 VBP denotes construct
T5976 7551-7557 VBN denotes served
T5977 7558-7560 IN denotes as
T5978 7561-7563 DT denotes an
T5979 7564-7572 JJ denotes internal
T5980 7573-7582 NN denotes reference
T5981 7583-7586 IN denotes for
T5982 7587-7599 NN denotes transfection
T5983 7600-7610 NN denotes efficiency
T5984 7610-7611 . denotes .
T5985 7612-7623 JJ denotes Forty-eight
T5986 7624-7629 NNS denotes hours
T5987 7630-7635 IN denotes after
T5988 7636-7648 NN denotes transfection
T5989 7648-7649 , denotes ,
T5990 7650-7660 NN denotes luciferase
T5991 7661-7669 NN denotes activity
T5992 7670-7673 VBD denotes was
T5993 7674-7682 VBN denotes measured
T5994 7683-7687 IN denotes with
T5995 7688-7689 DT denotes a
T5996 7690-7692 NNP denotes LB
T5997 7693-7695 CD denotes 96
T5998 7696-7697 NNP denotes P
T5999 7698-7708 NN denotes microlumat
T6000 7709-7710 -LRB- denotes (
T6001 7710-7714 NNP denotes EG&G
T6002 7715-7723 NNP denotes Berthold
T6003 7723-7724 , denotes ,
T6004 7725-7728 NNP denotes Bad
T6005 7729-7736 NNP denotes Wildbad
T6006 7736-7737 , denotes ,
T6007 7738-7745 NNP denotes Germany
T6008 7745-7746 -RRB- denotes )
T6009 7746-7747 . denotes .
T6010 7748-7753 CD denotes IRF-4
T6011 7754-7762 NN denotes promoter
T6012 7763-7773 NN denotes activation
T6013 7774-7777 VBD denotes was
T6014 7778-7788 VBN denotes quantified
T6015 7789-7791 IN denotes as
T6016 7792-7793 DT denotes a
T6017 7794-7799 NN denotes ratio
T6018 7800-7802 IN denotes of
T6019 7803-7811 VBN denotes measured
T6020 7812-7819 RB denotes firefly
T6021 7820-7825 JJ denotes light
T6022 7826-7831 NNS denotes units
T6023 7832-7833 -LRB- denotes (
T6024 7833-7836 NN denotes flu
T6025 7836-7837 -RRB- denotes )
T6026 7838-7846 JJ denotes relative
T6027 7847-7849 TO denotes to
T6028 7850-7857 NN denotes renilla
T6029 7858-7859 -LRB- denotes (
T6030 7859-7862 NN denotes rlu
T6031 7862-7863 -RRB- denotes )
T6032 7863-7864 . denotes .
T6033 7865-7869 DT denotes Each
T6034 7870-7880 NN denotes experiment
T6035 7881-7884 VBD denotes was
T6036 7885-7892 VBN denotes carried
T6037 7893-7896 RP denotes out
T6038 7897-7899 IN denotes at
T6039 7900-7905 JJS denotes least
T6040 7906-7911 CD denotes three
T6041 7912-7917 NNS denotes times
T6042 7917-7918 . denotes .
R2193 T2774 T2775 compound Cell,lines
R2194 T2775 T2782 nsubjpass lines,obtained
R2195 T2776 T2782 nsubjpass K-562,obtained
R2196 T2777 T2776 punct ",",K-562
R2197 T2778 T2776 conj Jurkat,K-562
R2198 T2779 T2778 cc and,Jurkat
R2199 T2780 T2778 conj U-937,Jurkat
R2200 T2781 T2782 auxpass were,obtained
R2201 T2782 T2782 ROOT obtained,obtained
R2202 T2783 T2782 prep from,obtained
R2203 T2784 T2785 det the,ATCC
R2204 T2785 T2783 pobj ATCC,from
R2205 T2786 T2785 punct (,ATCC
R2206 T2787 T2790 compound American,Collection
R2207 T2788 T2790 compound Type,Collection
R2208 T2789 T2790 compound Culture,Collection
R2209 T2790 T2785 appos Collection,ATCC
R2210 T2791 T2790 punct ",",Collection
R2211 T2792 T2790 conj Rockville,Collection
R2212 T2793 T2792 punct ",",Rockville
R2213 T2794 T2792 conj USA,Rockville
R2214 T2795 T2792 punct ),Rockville
R2215 T2796 T2790 cc and,Collection
R2216 T2797 T2790 conj EM-2,Collection
R2217 T2798 T2797 punct ",",EM-2
R2218 T2799 T2797 conj LAMA-84,EM-2
R2219 T2800 T2799 punct ",",LAMA-84
R2220 T2801 T2799 conj CML-T1,LAMA-84
R2221 T2802 T2801 punct ",",CML-T1
R2222 T2803 T2801 conj BV-173,CML-T1
R2223 T2804 T2803 punct ",",BV-173
R2224 T2805 T2803 conj SD-1,BV-173
R2225 T2806 T2805 cc and,SD-1
R2226 T2807 T2805 conj RPMI-8226,SD-1
R2227 T2808 T2807 prep from,RPMI-8226
R2228 T2809 T2810 det the,DSMZ
R2229 T2810 T2808 pobj DSMZ,from
R2230 T2811 T2810 punct (,DSMZ
R2231 T2812 T2816 compound Deutsche,und
R2232 T2813 T2816 compound Sammlung,und
R2233 T2814 T2815 compound von,Mikroorganismen
R2234 T2815 T2816 compound Mikroorganismen,und
R2235 T2816 T2818 compound und,GmbH
R2236 T2817 T2818 compound Zellkulturen,GmbH
R2237 T2818 T2807 conj GmbH,RPMI-8226
R2238 T2819 T2818 punct ",",GmbH
R2239 T2820 T2818 conj Braunschweig,GmbH
R2240 T2821 T2820 punct ",",Braunschweig
R2241 T2822 T2820 conj Germany,Braunschweig
R2242 T2823 T2818 punct ),GmbH
R2243 T2824 T2782 punct .,obtained
R2244 T2825 T2827 det All,lines
R2245 T2826 T2827 compound cell,lines
R2246 T2827 T2836 nsubj lines,were
R2247 T2828 T2827 punct ",",lines
R2248 T2829 T2827 prep except,lines
R2249 T2830 T2829 pobj BV-173,except
R2250 T2831 T2830 punct ",",BV-173
R2251 T2832 T2830 conj SD-1,BV-173
R2252 T2833 T2832 cc and,SD-1
R2253 T2834 T2832 conj RPMI-8226,SD-1
R2254 T2835 T2836 punct ",",were
R2255 T2836 T2836 ROOT were,were
R2256 T2837 T2836 acomp IRF-4-negative,were
R2257 T2838 T2836 punct .,were
R2349 T2949 T2950 compound Cell,culture
R2350 T2950 T2957 nsubjpass culture,maintained
R2351 T2951 T2950 cc and,culture
R2352 T2952 T2950 conj stimulation,culture
R2353 T2953 T2955 det All,lines
R2354 T2954 T2955 compound cell,lines
R2355 T2955 T2957 nsubjpass lines,maintained
R2356 T2956 T2957 auxpass were,maintained
R2357 T2957 T2957 ROOT maintained,maintained
R2358 T2958 T2957 prep at,maintained
R2359 T2959 T2960 nummod 5,%
R2360 T2960 T2958 pobj %,at
R2361 T2961 T2960 nummod CO2,%
R2362 T2962 T2961 prep in,CO2
R2363 T2963 T2965 compound RPMI,medium
R2364 T2964 T2965 compound 1640,medium
R2365 T2965 T2962 pobj medium,in
R2366 T2966 T2957 prep with,maintained
R2367 T2967 T2968 nummod 1,%
R2368 T2968 T2969 compound %,glutamine
R2369 T2969 T2966 pobj glutamine,with
R2370 T2970 T2969 punct (,glutamine
R2371 T2971 T2972 compound Gibco/BRL,Eggenstein
R2372 T2972 T2969 appos Eggenstein,glutamine
R2373 T2973 T2972 punct ",",Eggenstein
R2374 T2974 T2972 appos Germany,Eggenstein
R2375 T2975 T2976 punct ),supplemented
R2376 T2976 T2957 advcl supplemented,maintained
R2377 T2977 T2976 prep with,supplemented
R2378 T2978 T2979 nummod 10,%
R2379 T2979 T2982 compound %,serum
R2380 T2980 T2981 amod fetal,calf
R2381 T2981 T2982 compound calf,serum
R2382 T2982 T2977 pobj serum,with
R2383 T2983 T2982 punct (,serum
R2384 T2984 T2982 appos Gibco/BRL,serum
R2385 T2985 T2982 punct ),serum
R2386 T2986 T2982 punct ",",serum
R2387 T2987 T2988 nummod 1,%
R2388 T2988 T2989 compound %,penicillin/streptomycin
R2389 T2989 T2982 appos penicillin/streptomycin,serum
R2390 T2990 T2991 punct (,Biochrom
R2391 T2991 T2989 appos Biochrom,penicillin/streptomycin
R2392 T2992 T2991 punct ",",Biochrom
R2393 T2993 T2991 conj Berlin,Biochrom
R2394 T2994 T2993 punct ",",Berlin
R2395 T2995 T2993 conj Germany,Berlin
R2396 T2996 T2991 punct ),Biochrom
R2397 T2997 T2957 punct .,maintained
R2398 T2998 T2999 advmod When,indicated
R2399 T2999 T3003 advcl indicated,treated
R2400 T3000 T3003 punct ",",treated
R2401 T3001 T3003 nsubjpass cells,treated
R2402 T3002 T3003 auxpass were,treated
R2403 T3003 T3003 ROOT treated,treated
R2404 T3004 T3003 prep with,treated
R2405 T3005 T3004 pobj 5-aza-2-deoxycytidine,with
R2406 T3006 T3005 punct (,5-aza-2-deoxycytidine
R2407 T3007 T3005 appos AzadC,5-aza-2-deoxycytidine
R2408 T3008 T3007 punct ),AzadC
R2409 T3009 T3005 cc or,5-aza-2-deoxycytidine
R2410 T3010 T3005 conj 5-azacytidine,5-aza-2-deoxycytidine
R2411 T3011 T3012 punct (,AzaC
R2412 T3012 T3010 appos AzaC,5-azacytidine
R2413 T3013 T3012 punct ),AzaC
R2414 T3014 T3015 punct (,Sigma
R2415 T3015 T3010 appos Sigma,5-azacytidine
R2416 T3016 T3015 punct ",",Sigma
R2417 T3017 T3015 conj Taufkirchen,Sigma
R2418 T3018 T3017 punct ",",Taufkirchen
R2419 T3019 T3017 conj Germany,Taufkirchen
R2420 T3020 T3003 punct ),treated
R2421 T3021 T3003 prep for,treated
R2422 T3022 T3024 amod different,periods
R2423 T3023 T3024 compound time,periods
R2424 T3024 T3021 pobj periods,for
R2425 T3025 T3003 punct .,treated
R2426 T3026 T3026 ROOT Owing,Owing
R2427 T3027 T3026 prep to,Owing
R2428 T3028 T3030 poss their,instability
R2429 T3029 T3030 compound chemical,instability
R2430 T3030 T3033 dep instability,were
R2431 T3031 T3032 amod fresh,substances
R2432 T3032 T3033 nsubj substances,were
R2433 T3033 T3026 aux were,Owing
R2434 T3034 T3037 advmod re-added,h.
R2435 T3035 T3037 det every,h.
R2436 T3036 T3037 nummod 24,h.
R2437 T3037 T3033 attr h.,were
R2561 T3192 T3192 ROOT Sequencing,Sequencing
R2562 T3193 T3192 prep of,Sequencing
R2563 T3194 T3196 det the,promoter
R2564 T3195 T3196 compound IRF-4,promoter
R2565 T3196 T3193 pobj promoter,of
R2566 T3197 T3214 prep For,PCR-amplified
R2567 T3198 T3197 pobj analysis,For
R2568 T3199 T3198 prep of,analysis
R2569 T3200 T3203 det the,region
R2570 T3201 T3203 amod IRF-4,region
R2571 T3202 T3203 compound promoter,region
R2572 T3203 T3199 pobj region,of
R2573 T3204 T3198 prep for,analysis
R2574 T3205 T3206 amod permanent,aberrations
R2575 T3206 T3204 pobj aberrations,for
R2576 T3207 T3208 amod such,as
R2577 T3208 T3206 prep as,aberrations
R2578 T3209 T3208 pobj insertions/deletions,as
R2579 T3210 T3209 cc or,insertions/deletions
R2580 T3211 T3209 conj mutation,insertions/deletions
R2581 T3212 T3214 punct ",",PCR-amplified
R2582 T3213 T3214 nsubj we,PCR-amplified
R2583 T3214 T3214 ROOT PCR-amplified,PCR-amplified
R2584 T3215 T3216 nummod two,fragments
R2585 T3216 T3214 dobj fragments,PCR-amplified
R2586 T3217 T3216 prep from,fragments
R2587 T3218 T3219 amod genomic,DNA
R2588 T3219 T3217 pobj DNA,from
R2589 T3220 T3219 punct ",",DNA
R2590 T3221 T3223 nsubjpass which,extracted
R2591 T3222 T3223 auxpass was,extracted
R2592 T3223 T3216 relcl extracted,fragments
R2593 T3224 T3223 prep from,extracted
R2594 T3225 T3227 amod depicted,lines
R2595 T3226 T3227 compound cell,lines
R2596 T3227 T3224 pobj lines,from
R2597 T3228 T3223 prep with,extracted
R2598 T3229 T3231 det a,kit
R2599 T3230 T3231 amod commercial,kit
R2600 T3231 T3228 pobj kit,with
R2601 T3232 T3231 punct (,kit
R2602 T3233 T3231 appos Qiagen,kit
R2603 T3234 T3233 punct ",",Qiagen
R2604 T3235 T3233 conj Hilde,Qiagen
R2605 T3236 T3235 punct ",",Hilde
R2606 T3237 T3235 appos Germany,Hilde
R2607 T3238 T3235 punct ),Hilde
R2608 T3239 T3240 mark as,recommended
R2609 T3240 T3223 advcl recommended,extracted
R2610 T3241 T3214 punct .,PCR-amplified
R2611 T3242 T3243 det The,primers
R2612 T3243 T3244 nsubj primers,were
R2613 T3244 T3244 ROOT were,were
R2614 T3245 T3244 acomp 1-forward,were
R2615 T3246 T3247 punct :,5
R2616 T3247 T3251 nummod 5,′
R2617 T3248 T3250 punct ′,TTGAGATGGAGTCTTGCTCTGT-3
R2618 T3249 T3250 punct -,TTGAGATGGAGTCTTGCTCTGT-3
R2619 T3250 T3251 compound TTGAGATGGAGTCTTGCTCTGT-3,′
R2620 T3251 T3275 dep ′,′
R2621 T3252 T3251 punct ",",′
R2622 T3253 T3275 dep 1-reverse,′
R2623 T3254 T3255 punct :,5
R2624 T3255 T3253 nummod 5,1-reverse
R2625 T3256 T3255 punct ′,5
R2626 T3257 T3258 punct -,CCAGGACCTCAGGAGGCCAGTCA-3
R2627 T3258 T3259 compound CCAGGACCTCAGGAGGCCAGTCA-3,′
R2628 T3259 T3255 dep ′,5
R2629 T3260 T3275 punct ;,′
R2630 T3261 T3275 amod 2-forward,′
R2631 T3262 T3267 punct :,′
R2632 T3263 T3267 nummod 5,′
R2633 T3264 T3266 punct ′,AGCGGTGAAACTGAGAGTGCGAGGT-3
R2634 T3265 T3266 punct -,AGCGGTGAAACTGAGAGTGCGAGGT-3
R2635 T3266 T3267 compound AGCGGTGAAACTGAGAGTGCGAGGT-3,′
R2636 T3267 T3275 nmod ′,′
R2637 T3268 T3267 punct ",",′
R2638 T3269 T3275 nmod 2-reverse,′
R2639 T3270 T3271 punct :,5
R2640 T3271 T3269 nummod 5,2-reverse
R2641 T3272 T3274 punct ′,GCCACATCGCTGCAGTTTAG-3
R2642 T3273 T3274 punct -,GCCACATCGCTGCAGTTTAG-3
R2643 T3274 T3275 compound GCCACATCGCTGCAGTTTAG-3,′
R2644 T3275 T3244 acomp ′,were
R2645 T3276 T3244 punct .,were
R2646 T3277 T3278 det The,products
R2647 T3278 T3280 nsubjpass products,cloned
R2648 T3279 T3280 auxpass were,cloned
R2649 T3280 T3280 ROOT cloned,cloned
R2650 T3281 T3280 prep with,cloned
R2651 T3282 T3286 det the,kit
R2652 T3283 T3284 compound TOPO,TA
R2653 T3284 T3285 nsubj TA,cloning
R2654 T3285 T3286 amod cloning,kit
R2655 T3286 T3281 pobj kit,with
R2656 T3287 T3286 punct (,kit
R2657 T3288 T3286 appos Invitrogen,kit
R2658 T3289 T3288 punct ",",Invitrogen
R2659 T3290 T3288 conj Groningen,Invitrogen
R2660 T3291 T3290 punct ",",Groningen
R2661 T3292 T3293 det The,Netherlands
R2662 T3293 T3290 appos Netherlands,Groningen
R2663 T3294 T3290 punct ),Groningen
R2664 T3295 T3280 punct .,cloned
R2665 T3296 T3316 prep After,sequenced
R2666 T3297 T3298 amod bacterial,amplification
R2667 T3298 T3296 pobj amplification,After
R2668 T3299 T3298 prep of,amplification
R2669 T3300 T3303 det the,fragments
R2670 T3301 T3303 amod cloned,fragments
R2671 T3302 T3303 compound PCR,fragments
R2672 T3303 T3299 pobj fragments,of
R2673 T3304 T3298 prep by,amplification
R2674 T3305 T3306 amod standard,procedures
R2675 T3306 T3304 pobj procedures,by
R2676 T3307 T3316 punct ",",sequenced
R2677 T3308 T3309 advmod at,least
R2678 T3309 T3310 advmod least,three
R2679 T3310 T3311 nummod three,clones
R2680 T3311 T3316 nsubjpass clones,sequenced
R2681 T3312 T3311 prep from,clones
R2682 T3313 T3314 det each,sample
R2683 T3314 T3312 pobj sample,from
R2684 T3315 T3316 auxpass were,sequenced
R2685 T3316 T3316 ROOT sequenced,sequenced
R2686 T3317 T3316 prep with,sequenced
R2687 T3318 T3320 det an,sequencer
R2688 T3319 T3320 amod automated,sequencer
R2689 T3320 T3317 pobj sequencer,with
R2690 T3321 T3320 punct (,sequencer
R2691 T3322 T3323 compound ABI,Prism
R2692 T3323 T3320 appos Prism,sequencer
R2693 T3324 T3323 nummod 377,Prism
R2694 T3325 T3323 punct ",",Prism
R2695 T3326 T3327 compound Applied,Bio-systems
R2696 T3327 T3323 appos Bio-systems,Prism
R2697 T3328 T3323 punct ",",Prism
R2698 T3329 T3330 compound Foster,City
R2699 T3330 T3323 appos City,Prism
R2700 T3331 T3330 punct ",",City
R2701 T3332 T3330 npadvmod USA,City
R2702 T3333 T3320 punct ),sequencer
R2703 T3334 T3335 mark as,recommended
R2704 T3335 T3316 advcl recommended,sequenced
R2705 T3336 T3335 agent by,recommended
R2706 T3337 T3338 det the,manufacturer
R2707 T3338 T3336 pobj manufacturer,by
R2708 T3339 T3316 punct .,sequenced
R3088 T3802 T3803 compound Expression,analysis
R3089 T3803 T3813 nsubjpass analysis,extracted
R3090 T3804 T3805 aux To,analyze
R3091 T3805 T3813 advcl analyze,extracted
R3092 T3806 T3809 det the,level
R3093 T3807 T3809 nmod IRF-4,level
R3094 T3808 T3809 amod transcriptional,level
R3095 T3809 T3805 dobj level,analyze
R3096 T3810 T3813 punct ",",extracted
R3097 T3811 T3813 nsubjpass RNA,extracted
R3098 T3812 T3813 auxpass was,extracted
R3099 T3813 T3813 ROOT extracted,extracted
R3100 T3814 T3813 prep from,extracted
R3101 T3815 T3814 pobj cells,from
R3102 T3816 T3813 advcl using,extracted
R3103 T3817 T3819 det the,RNAzol-kit
R3104 T3818 T3819 amod commercial,RNAzol-kit
R3105 T3819 T3816 dobj RNAzol-kit,using
R3106 T3820 T3819 punct (,RNAzol-kit
R3107 T3821 T3819 appos Paesel,RNAzol-kit
R3108 T3822 T3821 punct ",",Paesel
R3109 T3823 T3821 npadvmod Frankfurt,Paesel
R3110 T3824 T3823 punct ",",Frankfurt
R3111 T3825 T3823 conj Germany,Frankfurt
R3112 T3826 T3821 punct ),Paesel
R3113 T3827 T3813 punct .,extracted
R3114 T3828 T3829 det An,aliquot
R3115 T3829 T3836 nsubjpass aliquot,used
R3116 T3830 T3829 prep of,aliquot
R3117 T3831 T3832 nummod 1,μg
R3118 T3832 T3830 pobj μg,of
R3119 T3833 T3834 amod total,RNA
R3120 T3834 T3829 appos RNA,aliquot
R3121 T3835 T3836 auxpass was,used
R3122 T3836 T3836 ROOT used,used
R3123 T3837 T3836 prep for,used
R3124 T3838 T3839 compound cDNA,synthesis
R3125 T3839 T3837 pobj synthesis,for
R3126 T3840 T3841 mark as,described
R3127 T3841 T3836 advcl described,used
R3128 T3842 T3841 advmod previously,described
R3129 T3843 T3844 punct (,27
R3130 T3844 T3841 npadvmod 27,described
R3131 T3845 T3836 punct ),used
R3132 T3846 T3836 punct .,used
R3133 T3847 T3849 compound RNA,analysis
R3134 T3848 T3849 compound expression,analysis
R3135 T3849 T3858 nsubjpass analysis,carried
R3136 T3850 T3849 prep for,analysis
R3137 T3851 T3850 pobj IRF-4,for
R3138 T3852 T3851 cc and,IRF-4
R3139 T3853 T3855 det the,gene
R3140 T3854 T3855 compound reference,gene
R3141 T3855 T3856 compound gene,β-actin
R3142 T3856 T3858 nsubjpass β-actin,carried
R3143 T3857 T3858 auxpass was,carried
R3144 T3858 T3858 ROOT carried,carried
R3145 T3859 T3858 prt out,carried
R3146 T3860 T3858 agent by,carried
R3147 T3861 T3862 compound semi-quantitative,PCR
R3148 T3862 T3860 pobj PCR,by
R3149 T3863 T3864 mark as,described
R3150 T3864 T3858 advcl described,carried
R3151 T3865 T3864 advmod previously,described
R3152 T3866 T3864 punct (,described
R3153 T3867 T3864 npadvmod "3,27",described
R3154 T3868 T3864 punct ),described
R3155 T3869 T3858 punct .,carried
R3156 T3870 T3871 compound PCR,products
R3157 T3871 T3873 nsubjpass products,verified
R3158 T3872 T3873 auxpass were,verified
R3159 T3873 T3873 ROOT verified,verified
R3160 T3874 T3873 agent by,verified
R3161 T3875 T3876 amod automated,sequencing
R3162 T3876 T3874 pobj sequencing,by
R3163 T3877 T3873 punct .,verified
R3164 T3878 T3879 compound PCR,primers
R3165 T3879 T3904 nsubjpass primers,published
R3166 T3880 T3879 cc and,primers
R3167 T3881 T3879 conj conditions,primers
R3168 T3882 T3879 prep for,primers
R3169 T3883 T3884 compound expression,analysis
R3170 T3884 T3882 pobj analysis,for
R3171 T3885 T3884 prep of,analysis
R3172 T3886 T3885 pobj DNMT,of
R3173 T3887 T3886 cc or,DNMT
R3174 T3888 T3886 conj MBP,DNMT
R3175 T3889 T3888 punct (,MBP
R3176 T3890 T3891 compound DNMT1,DNMT3A
R3177 T3891 T3888 appos DNMT3A,MBP
R3178 T3892 T3891 punct ",",DNMT3A
R3179 T3893 T3891 conj DNMT3B,DNMT3A
R3180 T3894 T3893 punct ",",DNMT3B
R3181 T3895 T3893 conj MeCP,DNMT3B
R3182 T3896 T3895 punct ",",MeCP
R3183 T3897 T3895 conj MBD1,MeCP
R3184 T3898 T3897 punct ",",MBD1
R3185 T3899 T3897 conj MBD2,MBD1
R3186 T3900 T3899 cc and,MBD2
R3187 T3901 T3899 conj MBD4,MBD2
R3188 T3902 T3888 punct ),MBP
R3189 T3903 T3904 auxpass were,published
R3190 T3904 T3904 ROOT published,published
R3191 T3905 T3904 advmod elsewhere,published
R3192 T3906 T3904 punct (,published
R3193 T3907 T3904 npadvmod 28,published
R3194 T3908 T3904 punct ),published
R3195 T3909 T3904 punct .,published
R3196 T3910 T3922 prep For,performed
R3197 T3911 T3910 pobj analysis,For
R3198 T3912 T3911 prep of,analysis
R3199 T3913 T3915 compound IRF-4,expression
R3200 T3914 T3915 compound protein,expression
R3201 T3915 T3912 pobj expression,of
R3202 T3916 T3922 punct ",",performed
R3203 T3917 T3920 det a,assay
R3204 T3918 T3920 amod standard,assay
R3205 T3919 T3920 compound immunoblotting,assay
R3206 T3920 T3922 nsubjpass assay,performed
R3207 T3921 T3922 auxpass was,performed
R3208 T3922 T3922 ROOT performed,performed
R3209 T3923 T3924 mark as,described
R3210 T3924 T3922 advcl described,performed
R3211 T3925 T3924 advmod previously,described
R3212 T3926 T3927 punct (,29
R3213 T3927 T3924 npadvmod 29,described
R3214 T3928 T3922 punct ),performed
R3215 T3929 T3922 punct .,performed
R3216 T3930 T3935 advmod Briefly,generated
R3217 T3931 T3935 punct ",",generated
R3218 T3932 T3933 compound protein,lysates
R3219 T3933 T3935 nsubjpass lysates,generated
R3220 T3934 T3935 auxpass were,generated
R3221 T3935 T3935 ROOT generated,generated
R3222 T3936 T3935 prep by,generated
R3223 T3937 T3936 pcomp incubating,by
R3224 T3938 T3941 nummod 1,cells
R3225 T3939 T3941 nummod ×,cells
R3226 T3940 T3941 nummod 106,cells
R3227 T3941 T3937 dobj cells,incubating
R3228 T3942 T3937 prep in,incubating
R3229 T3943 T3944 nummod 100,µl
R3230 T3944 T3946 compound µl,buffer
R3231 T3945 T3946 compound RIPA,buffer
R3232 T3946 T3942 pobj buffer,in
R3233 T3947 T3950 punct (,NP-40
R3234 T3948 T3949 nummod 1,%
R3235 T3949 T3950 compound %,NP-40
R3236 T3950 T3946 appos NP-40,buffer
R3237 T3951 T3950 punct ",",NP-40
R3238 T3952 T3953 nummod 0.5,%
R3239 T3953 T3954 compound %,sodiumdesoxycholate
R3240 T3954 T3950 appos sodiumdesoxycholate,NP-40
R3241 T3955 T3954 punct ",",sodiumdesoxycholate
R3242 T3956 T3957 nummod 0.1,%
R3243 T3957 T3958 compound %,SDS
R3244 T3958 T3950 appos SDS,NP-40
R3245 T3959 T3958 punct ",",SDS
R3246 T3960 T3961 nummod 100,µg
R3247 T3961 T3965 nmod µg,fluoride
R3248 T3962 T3965 nmod /,fluoride
R3249 T3963 T3965 nmod ml,fluoride
R3250 T3964 T3965 compound phenylmethylsulfonyl,fluoride
R3251 T3965 T3958 appos fluoride,SDS
R3252 T3966 T3965 punct ",",fluoride
R3253 T3967 T3968 nummod 10,µl
R3254 T3968 T3971 nmod µl,protease-inhibitory-mix
R3255 T3969 T3971 nmod /,protease-inhibitory-mix
R3256 T3970 T3971 compound ml,protease-inhibitory-mix
R3257 T3971 T3965 conj protease-inhibitory-mix,fluoride
R3258 T3972 T3971 punct ",",protease-inhibitory-mix
R3259 T3973 T3975 quantmod 1,/
R3260 T3974 T3975 quantmod µmol,/
R3261 T3975 T3977 nummod /,sodiumorthovanadate
R3262 T3976 T3977 compound ml,sodiumorthovanadate
R3263 T3977 T3971 appos sodiumorthovanadate,protease-inhibitory-mix
R3264 T3978 T3977 prep in,sodiumorthovanadate
R3265 T3979 T3980 amod phosphate-buffered,saline
R3266 T3980 T3978 pobj saline,in
R3267 T3981 T3971 punct ),protease-inhibitory-mix
R3268 T3982 T3965 prep for,fluoride
R3269 T3983 T3984 nummod 30,min
R3270 T3984 T3982 pobj min,for
R3271 T3985 T3984 prep on,min
R3272 T3986 T3985 pobj ice,on
R3273 T3987 T3935 punct .,generated
R3274 T3988 T3997 prep After,determined
R3275 T3989 T3988 pobj centrifugation,After
R3276 T3990 T3997 punct ",",determined
R3277 T3991 T3992 compound protein,concentration
R3278 T3992 T3997 nsubjpass concentration,determined
R3279 T3993 T3992 prep of,concentration
R3280 T3994 T3995 det the,supernatant
R3281 T3995 T3993 pobj supernatant,of
R3282 T3996 T3997 auxpass was,determined
R3283 T3997 T3997 ROOT determined,determined
R3284 T3998 T3997 agent by,determined
R3285 T3999 T4001 amod BCA-method,Pierce
R3286 T4000 T4001 punct (,Pierce
R3287 T4001 T3998 pobj Pierce,by
R3288 T4002 T4001 punct ",",Pierce
R3289 T4003 T4006 nmod Rockford,)
R3290 T4004 T4003 punct ",",Rockford
R3291 T4005 T4003 conj IL,Rockford
R3292 T4006 T4001 punct ),Pierce
R3293 T4007 T4008 mark as,recommended
R3294 T4008 T4001 advcl recommended,Pierce
R3295 T4009 T3997 punct .,determined
R3296 T4010 T4011 compound Protein,lysates
R3297 T4011 T4018 nsubjpass lysates,electrophoresed
R3298 T4012 T4011 punct (,lysates
R3299 T4013 T4011 appos 70,lysates
R3300 T4014 T4015 nummod 100,µg
R3301 T4015 T4013 appos µg,70
R3302 T4016 T4013 punct ),70
R3303 T4017 T4018 auxpass were,electrophoresed
R3304 T4018 T4018 ROOT electrophoresed,electrophoresed
R3305 T4019 T4018 prep on,electrophoresed
R3306 T4020 T4021 compound polyacrylamide,gels
R3307 T4021 T4019 pobj gels,on
R3308 T4022 T4018 cc and,electrophoresed
R3309 T4023 T4018 conj transferred,electrophoresed
R3310 T4024 T4023 prep to,transferred
R3311 T4025 T4026 det a,PVDF-membrane
R3312 T4026 T4024 pobj PVDF-membrane,to
R3313 T4027 T4029 punct (,P
R3314 T4028 T4029 compound Immobilon,P
R3315 T4029 T4026 appos P,PVDF-membrane
R3316 T4030 T4029 punct ",",P
R3317 T4031 T4032 nummod 0.45,µm
R3318 T4032 T4029 appos µm,P
R3319 T4033 T4029 punct ;,P
R3320 T4034 T4029 conj Millipore,P
R3321 T4035 T4034 punct ",",Millipore
R3322 T4036 T4034 conj Eschborn,Millipore
R3323 T4037 T4036 punct ",",Eschborn
R3324 T4038 T4036 conj Germany,Eschborn
R3325 T4039 T4029 punct ),P
R3326 T4040 T4018 punct .,electrophoresed
R3327 T4041 T4043 nsubjpass Membranes,blocked
R3328 T4042 T4043 auxpass were,blocked
R3329 T4043 T4043 ROOT blocked,blocked
R3330 T4044 T4043 prep with,blocked
R3331 T4045 T4046 nummod 2.5,%
R3332 T4046 T4047 nsubj %,blocking
R3333 T4047 T4044 pcomp blocking,with
R3334 T4048 T4047 dobj reagent,blocking
R3335 T4049 T4051 punct (,Mannheim
R3336 T4050 T4051 compound Boehringer,Mannheim
R3337 T4051 T4086 nsubj Mannheim,incubated
R3338 T4052 T4051 punct ",",Mannheim
R3339 T4053 T4051 npadvmod Germany,Mannheim
R3340 T4054 T4051 punct ),Mannheim
R3341 T4055 T4051 prep in,Mannheim
R3342 T4056 T4057 compound TBST,buffer
R3343 T4057 T4055 pobj buffer,in
R3344 T4058 T4051 punct (,Mannheim
R3345 T4059 T4060 nummod 4.44,g/l
R3346 T4060 T4061 compound g/l,Tris
R3347 T4061 T4051 appos Tris,Mannheim
R3348 T4062 T4051 appos HCL,Mannheim
R3349 T4063 T4062 punct ",",HCL
R3350 T4064 T4065 nummod 2.65,g/l
R3351 T4065 T4066 compound g/l,TrisOH
R3352 T4066 T4051 appos TrisOH,Mannheim
R3353 T4067 T4051 punct ",",Mannheim
R3354 T4068 T4069 nummod 8.07,g/l
R3355 T4069 T4070 compound g/l,NaCl
R3356 T4070 T4051 appos NaCl,Mannheim
R3357 T4071 T4070 punct ",",NaCl
R3358 T4072 T4073 nummod 0.2,g/l
R3359 T4073 T4074 compound g/l,KCl
R3360 T4074 T4070 conj KCl,NaCl
R3361 T4075 T4074 cc and,KCl
R3362 T4076 T4077 nummod 500,µl
R3363 T4077 T4078 compound µl,/
R3364 T4078 T4080 compound /,Tween-20
R3365 T4079 T4080 compound l,Tween-20
R3366 T4080 T4074 conj Tween-20,KCl
R3367 T4081 T4080 prep in,Tween-20
R3368 T4082 T4081 pobj H2O,in
R3369 T4083 T4080 punct ),Tween-20
R3370 T4084 T4080 cc and,Tween-20
R3371 T4085 T4086 advmod subsequently,incubated
R3372 T4086 T4047 advcl incubated,blocking
R3373 T4087 T4086 prep with,incubated
R3374 T4088 T4089 amod primary,antibody
R3375 T4089 T4087 pobj antibody,with
R3376 T4090 T4091 mark as,indicated
R3377 T4091 T4086 advcl indicated,incubated
R3378 T4092 T4091 cc and,indicated
R3379 T4093 T4096 amod horseradish,antibody
R3380 T4094 T4096 amod peroxidase-conjugated,antibody
R3381 T4095 T4096 amod secondary,antibody
R3382 T4096 T4103 nmod antibody,DAKO
R3383 T4097 T4096 punct ",",antibody
R3384 T4098 T4096 conj anti-mouse,antibody
R3385 T4099 T4098 cc or,anti-mouse
R3386 T4100 T4098 conj anti-goat,anti-mouse
R3387 T4101 T4103 nmod IgG,DAKO
R3388 T4102 T4103 punct (,DAKO
R3389 T4103 T4086 conj DAKO,incubated
R3390 T4104 T4103 punct ",",DAKO
R3391 T4105 T4103 conj Hamburg,DAKO
R3392 T4106 T4105 punct ",",Hamburg
R3393 T4107 T4105 conj Germany,Hamburg
R3394 T4108 T4105 punct ),Hamburg
R3395 T4109 T4103 punct ",",DAKO
R3396 T4110 T4103 npadvmod respectively,DAKO
R3397 T4111 T4043 punct .,blocked
R3398 T4112 T4113 det The,membranes
R3399 T4113 T4116 nsubjpass membranes,developed
R3400 T4114 T4116 auxpass were,developed
R3401 T4115 T4116 advmod then,developed
R3402 T4116 T4116 ROOT developed,developed
R3403 T4117 T4116 prep with,developed
R3404 T4118 T4121 det an,kit
R3405 T4119 T4121 compound ECL,kit
R3406 T4120 T4121 compound detection,kit
R3407 T4121 T4117 pobj kit,with
R3408 T4122 T4121 punct (,kit
R3409 T4123 T4125 compound Amersham,Biotech
R3410 T4124 T4125 compound Pharmacia,Biotech
R3411 T4125 T4121 appos Biotech,kit
R3412 T4126 T4125 punct ",",Biotech
R3413 T4127 T4125 conj Freiburg,Biotech
R3414 T4128 T4127 punct ",",Freiburg
R3415 T4129 T4127 appos Germany,Freiburg
R3416 T4130 T4127 punct ),Freiburg
R3417 T4131 T4116 punct .,developed
R3418 T4132 T4134 det The,antibodies
R3419 T4133 T4134 amod primary,antibodies
R3420 T4134 T4135 nsubj antibodies,were
R3421 T4135 T4135 ROOT were,were
R3422 T4136 T4139 amod goat,ICSAT
R3423 T4137 T4139 amod anti-IRF-4,ICSAT
R3424 T4138 T4139 compound /,ICSAT
R3425 T4139 T4135 attr ICSAT,were
R3426 T4140 T4139 punct (,ICSAT
R3427 T4141 T4139 appos M-17,ICSAT
R3428 T4142 T4139 punct ),ICSAT
R3429 T4143 T4146 punct (,Biotechnology
R3430 T4144 T4145 compound Santa,Cruz
R3431 T4145 T4146 compound Cruz,Biotechnology
R3432 T4146 T4139 conj Biotechnology,ICSAT
R3433 T4147 T4146 punct ",",Biotechnology
R3434 T4148 T4149 compound Santa,Cruz
R3435 T4149 T4146 conj Cruz,Biotechnology
R3436 T4150 T4149 punct ",",Cruz
R3437 T4151 T4149 conj CA,Cruz
R3438 T4152 T4146 punct ),Biotechnology
R3439 T4153 T4146 cc and,Biotechnology
R3440 T4154 T4155 compound mouse,anti-β-actin
R3441 T4155 T4139 conj anti-β-actin,ICSAT
R3442 T4156 T4157 punct (,AC-74
R3443 T4157 T4155 appos AC-74,anti-β-actin
R3444 T4158 T4155 punct ),anti-β-actin
R3445 T4159 T4160 punct (,Sigma
R3446 T4160 T4139 appos Sigma,ICSAT
R3447 T4161 T4139 punct ),ICSAT
R3448 T4162 T4135 punct .,were
R3780 T4626 T4628 amod Methylation-specific,DNA
R3781 T4627 T4628 amod restriction-PCR-assay,DNA
R3782 T4628 T4630 nsubjpass DNA,extracted
R3783 T4629 T4630 auxpass was,extracted
R3784 T4630 T4630 ROOT extracted,extracted
R3785 T4631 T4630 prep with,extracted
R3786 T4632 T4634 det a,kit
R3787 T4633 T4634 amod commercial,kit
R3788 T4634 T4631 pobj kit,with
R3789 T4635 T4634 punct (,kit
R3790 T4636 T4634 appos Qiagen,kit
R3791 T4637 T4634 punct ),kit
R3792 T4638 T4639 mark as,recommended
R3793 T4639 T4630 advcl recommended,extracted
R3794 T4640 T4630 punct .,extracted
R3795 T4641 T4649 mark Since,inhibited
R3796 T4642 T4644 det the,ability
R3797 T4643 T4644 compound restriction,ability
R3798 T4644 T4649 nsubjpass ability,inhibited
R3799 T4645 T4644 prep of,ability
R3800 T4646 T4647 amod several,endonucleases
R3801 T4647 T4645 pobj endonucleases,of
R3802 T4648 T4649 auxpass is,inhibited
R3803 T4649 T4658 advcl inhibited,used
R3804 T4650 T4649 agent by,inhibited
R3805 T4651 T4650 pobj methylation,by
R3806 T4652 T4651 prep of,methylation
R3807 T4653 T4655 poss their,sequence
R3808 T4654 T4655 compound target,sequence
R3809 T4655 T4652 pobj sequence,of
R3810 T4656 T4658 punct ",",used
R3811 T4657 T4658 nsubj we,used
R3812 T4658 T4658 ROOT used,used
R3813 T4659 T4660 amod methylation-sensitive,enzymes
R3814 T4660 T4658 dobj enzymes,used
R3815 T4661 T4660 appos HpaII,enzymes
R3816 T4662 T4661 cc and,HpaII
R3817 T4663 T4664 compound HaeII-isochizomer,Bsp143II
R3818 T4664 T4661 conj Bsp143II,HpaII
R3819 T4665 T4664 cc and,Bsp143II
R3820 T4666 T4664 conj Bsh1236I,Bsp143II
R3821 T4667 T4669 punct (,Fermentas
R3822 T4668 T4669 compound MBI,Fermentas
R3823 T4669 T4664 conj Fermentas,Bsp143II
R3824 T4670 T4669 punct ",",Fermentas
R3825 T4671 T4672 compound St,Leon-Rot
R3826 T4672 T4669 conj Leon-Rot,Fermentas
R3827 T4673 T4672 punct ",",Leon-Rot
R3828 T4674 T4672 appos Germany,Leon-Rot
R3829 T4675 T4672 punct ),Leon-Rot
R3830 T4676 T4677 punct (,"20,24"
R3831 T4677 T4672 appos "20,24",Leon-Rot
R3832 T4678 T4677 punct ),"20,24"
R3833 T4679 T4658 punct .,used
R3834 T4680 T4701 prep As,used
R3835 T4681 T4680 pobj control,As
R3836 T4682 T4684 det the,enzyme
R3837 T4683 T4684 compound methylation-resistant,enzyme
R3838 T4684 T4701 nsubjpass enzyme,used
R3839 T4685 T4684 appos MspI,enzyme
R3840 T4686 T4685 cc and,MspI
R3841 T4687 T4688 det an,enzyme
R3842 T4688 T4685 conj enzyme,MspI
R3843 T4689 T4688 prep with,enzyme
R3844 T4690 T4692 det no,site
R3845 T4691 T4692 compound recognition,site
R3846 T4692 T4689 pobj site,with
R3847 T4693 T4692 prep in,site
R3848 T4694 T4696 det the,promoter
R3849 T4695 T4696 compound target,promoter
R3850 T4696 T4693 pobj promoter,in
R3851 T4697 T4696 punct ",",promoter
R3852 T4698 T4696 appos EcoRI,promoter
R3853 T4699 T4701 punct ",",used
R3854 T4700 T4701 auxpass were,used
R3855 T4701 T4701 ROOT used,used
R3856 T4702 T4701 punct .,used
R3857 T4703 T4709 nsubjpass DNA,digested
R3858 T4704 T4706 punct (,µg
R3859 T4705 T4706 nummod 0.8,µg
R3860 T4706 T4703 appos µg,DNA
R3861 T4707 T4706 punct ),µg
R3862 T4708 T4709 auxpass was,digested
R3863 T4709 T4709 ROOT digested,digested
R3864 T4710 T4709 agent by,digested
R3865 T4711 T4715 nummod 40,enzyme
R3866 T4712 T4715 nmod U,enzyme
R3867 T4713 T4715 det the,enzyme
R3868 T4714 T4715 amod respective,enzyme
R3869 T4715 T4710 pobj enzyme,by
R3870 T4716 T4709 prep for,digested
R3871 T4717 T4718 nummod 6,h
R3872 T4718 T4716 pobj h,for
R3873 T4719 T4718 cc and,h
R3874 T4720 T4718 punct ",",h
R3875 T4721 T4722 aux to,ensure
R3876 T4722 T4709 advcl ensure,digested
R3877 T4723 T4724 amod complete,cleavage
R3878 T4724 T4722 dobj cleavage,ensure
R3879 T4725 T4724 punct ",",cleavage
R3880 T4726 T4728 amod additional,U
R3881 T4727 T4728 nummod 20,U
R3882 T4728 T4724 appos U,cleavage
R3883 T4729 T4722 prep for,ensure
R3884 T4730 T4731 nummod 16,h.
R3885 T4731 T4729 pobj h.,for
R3886 T4732 T4739 advmod Thereafter,used
R3887 T4733 T4734 nummod 100,ng
R3888 T4734 T4739 nsubjpass ng,used
R3889 T4735 T4734 prep of,ng
R3890 T4736 T4737 amod digested,DNA
R3891 T4737 T4735 pobj DNA,of
R3892 T4738 T4739 auxpass was,used
R3893 T4739 T4739 ROOT used,used
R3894 T4740 T4739 prep to,used
R3895 T4741 T4743 det a,amplification
R3896 T4742 T4743 compound PCR,amplification
R3897 T4743 T4740 pobj amplification,to
R3898 T4744 T4743 prep of,amplification
R3899 T4745 T4746 nummod two,fragments
R3900 T4746 T4744 pobj fragments,of
R3901 T4747 T4746 punct (,fragments
R3902 T4748 T4746 appos F1,fragments
R3903 T4749 T4748 cc and,F1
R3904 T4750 T4748 conj F2,F1
R3905 T4751 T4746 punct ),fragments
R3906 T4752 T4743 acl spanning,amplification
R3907 T4753 T4752 dobj part,spanning
R3908 T4754 T4753 prep of,part
R3909 T4755 T4757 det the,promoter
R3910 T4756 T4757 compound IRF-4,promoter
R3911 T4757 T4754 pobj promoter,of
R3912 T4758 T4757 punct (,promoter
R3913 T4759 T4757 appos 30,promoter
R3914 T4760 T4757 punct ),promoter
R3915 T4761 T4763 punct (,U52683
R3916 T4762 T4763 compound GenBank,U52683
R3917 T4763 T4743 appos U52683,amplification
R3918 T4764 T4765 punct ;,see
R3919 T4765 T4765 ROOT see,see
R3920 T4766 T4767 compound Figure,3A
R3921 T4767 T4765 dobj 3A,see
R3922 T4768 T4765 punct ),see
R3923 T4769 T4765 punct .,see
R3924 T4770 T4771 det The,sequences
R3925 T4771 T4775 nsubj sequences,were
R3926 T4772 T4771 prep of,sequences
R3927 T4773 T4774 det the,primers
R3928 T4774 T4772 pobj primers,of
R3929 T4775 T4775 ROOT were,were
R3930 T4776 T4775 acomp F1-forward,were
R3931 T4777 T4775 punct :,were
R3932 T4778 T4782 nummod 5,′
R3933 T4779 T4781 punct ′,TTGAGATGGAGTCTTGCTCTGT-3
R3934 T4780 T4781 punct -,TTGAGATGGAGTCTTGCTCTGT-3
R3935 T4781 T4782 compound TTGAGATGGAGTCTTGCTCTGT-3,′
R3936 T4782 T4793 npadvmod ′,F2-forward
R3937 T4783 T4784 punct ",",F1-reverse
R3938 T4784 T4793 nmod F1-reverse,F2-forward
R3939 T4785 T4784 punct :,F1-reverse
R3940 T4786 T4787 compound ATCACTTCCAGACTTCAGTTCACCT-3,′
R3941 T4787 T4784 appos ′,F1-reverse
R3942 T4788 T4790 punct (,bp
R3943 T4789 T4790 nummod 341,bp
R3944 T4790 T4787 appos bp,′
R3945 T4791 T4787 punct ),′
R3946 T4792 T4793 punct ;,F2-forward
R3947 T4793 T4775 dep F2-forward,were
R3948 T4794 T4793 punct :,F2-forward
R3949 T4795 T4799 nummod 5,′
R3950 T4796 T4798 punct ′,AAGGTGAACTGAAGTCTGGAAGTGA-3
R3951 T4797 T4798 punct -,AAGGTGAACTGAAGTCTGGAAGTGA-3
R3952 T4798 T4799 compound AAGGTGAACTGAAGTCTGGAAGTGA-3,′
R3953 T4799 T4793 appos ′,F2-forward
R3954 T4800 T4799 punct ",",′
R3955 T4801 T4799 conj F2-reverse,′
R3956 T4802 T4801 punct :,F2-reverse
R3957 T4803 T4807 nummod 5,′
R3958 T4804 T4806 punct ′,CCAGGACCTCAGGAGGCCAGTCA-3
R3959 T4805 T4806 punct -,CCAGGACCTCAGGAGGCCAGTCA-3
R3960 T4806 T4807 compound CCAGGACCTCAGGAGGCCAGTCA-3,′
R3961 T4807 T4801 appos ′,F2-reverse
R3962 T4808 T4810 punct (,bp
R3963 T4809 T4810 nummod 474,bp
R3964 T4810 T4807 appos bp,′
R3965 T4811 T4807 punct ),′
R3966 T4812 T4775 punct .,were
R3967 T4813 T4815 det The,conditions
R3968 T4814 T4815 compound PCR,conditions
R3969 T4815 T4817 nsubjpass conditions,described
R3970 T4816 T4817 auxpass were,described
R3971 T4817 T4817 ROOT described,described
R3972 T4818 T4817 advmod elsewhere,described
R3973 T4819 T4820 punct (,3
R3974 T4820 T4817 parataxis 3,described
R3975 T4821 T4820 punct ),3
R3976 T4822 T4817 punct .,described
R3977 T4823 T4825 nsubjpass PCR,performed
R3978 T4824 T4825 auxpass was,performed
R3979 T4825 T4825 ROOT performed,performed
R3980 T4826 T4825 prep with,performed
R3981 T4827 T4829 det an,temperature
R3982 T4828 T4829 compound annealing,temperature
R3983 T4829 T4826 pobj temperature,with
R3984 T4830 T4829 prep of,temperature
R3985 T4831 T4833 nummod 62,C
R3986 T4832 T4833 nummod °,C
R3987 T4833 T4830 pobj C,of
R3988 T4834 T4829 cc and,temperature
R3989 T4835 T4836 nummod 35,cycles
R3990 T4836 T4829 conj cycles,temperature
R3991 T4837 T4825 punct .,performed
R3992 T4838 T4841 advmod When,methylated
R3993 T4839 T4841 nsubjpass DNA,methylated
R3994 T4840 T4841 auxpass was,methylated
R3995 T4841 T4849 advcl methylated,were
R3996 T4842 T4841 prep at,methylated
R3997 T4843 T4844 amod specific,sites
R3998 T4844 T4842 pobj sites,at
R3999 T4845 T4849 punct ",",were
R4000 T4846 T4848 det the,enzymes
R4001 T4847 T4848 amod sensitive,enzymes
R4002 T4848 T4849 nsubj enzymes,were
R4003 T4849 T4869 ccomp were,digested
R4004 T4850 T4849 neg not,were
R4005 T4851 T4849 acomp able,were
R4006 T4852 T4853 aux to,digest
R4007 T4853 T4851 xcomp digest,able
R4008 T4854 T4855 det the,DNA
R4009 T4855 T4853 dobj DNA,digest
R4010 T4856 T4855 cc and,DNA
R4011 T4857 T4855 conj amplification,DNA
R4012 T4858 T4849 conj took,were
R4013 T4859 T4858 dobj place,took
R4014 T4860 T4869 punct ;,digested
R4015 T4861 T4869 prep in,digested
R4016 T4862 T4861 pobj case,in
R4017 T4863 T4862 prep of,case
R4018 T4864 T4865 det no,methylation
R4019 T4865 T4863 pobj methylation,of
R4020 T4866 T4869 punct ",",digested
R4021 T4867 T4869 nsubjpass DNA,digested
R4022 T4868 T4869 auxpass was,digested
R4023 T4869 T4869 ROOT digested,digested
R4024 T4870 T4869 cc and,digested
R4025 T4871 T4872 det no,product
R4026 T4872 T4874 nsubjpass product,generated
R4027 T4873 T4874 auxpass was,generated
R4028 T4874 T4869 conj generated,digested
R4029 T4875 T4874 punct .,generated
R4030 T4876 T4878 det The,products
R4031 T4877 T4878 compound PCR,products
R4032 T4878 T4880 nsubjpass products,electrophoresed
R4033 T4879 T4880 auxpass were,electrophoresed
R4034 T4880 T4880 ROOT electrophoresed,electrophoresed
R4035 T4881 T4880 prep on,electrophoresed
R4036 T4882 T4886 det a,gel
R4037 T4883 T4884 nummod 3,%
R4038 T4884 T4886 nmod %,gel
R4039 T4885 T4886 amod agarose,gel
R4040 T4886 T4881 pobj gel,on
R4041 T4887 T4889 punct ",",stained
R4042 T4888 T4889 auxpass were,stained
R4043 T4889 T4880 conj stained,electrophoresed
R4044 T4890 T4889 prep with,stained
R4045 T4891 T4892 compound ethidium,bromide
R4046 T4892 T4890 pobj bromide,with
R4047 T4893 T4889 cc and,stained
R4048 T4894 T4889 conj photographed,stained
R4049 T4895 T4889 punct .,stained
R4050 T4896 T4897 compound PCR,products
R4051 T4897 T4899 nsubjpass products,verified
R4052 T4898 T4899 auxpass were,verified
R4053 T4899 T4899 ROOT verified,verified
R4054 T4900 T4899 agent by,verified
R4055 T4901 T4902 amod automated,sequencing
R4056 T4902 T4900 pobj sequencing,by
R4057 T4903 T4899 punct .,verified
R4386 T5272 T5274 compound Bisulfite,DNA
R4387 T5273 T5274 compound treatment,DNA
R4388 T5274 T5276 nsubjpass DNA,extracted
R4389 T5275 T5276 auxpass was,extracted
R4390 T5276 T5276 ROOT extracted,extracted
R4391 T5277 T5278 mark as,described
R4392 T5278 T5276 advcl described,extracted
R4393 T5279 T5278 advmod above,described
R4394 T5280 T5276 punct .,extracted
R4395 T5281 T5282 amod Bisulfite,treatment
R4396 T5282 T5304 advcl treatment,performed
R4397 T5283 T5282 prep of,treatment
R4398 T5284 T5283 pobj DNA,of
R4399 T5285 T5282 punct ",",treatment
R4400 T5286 T5304 advcl leading,performed
R4401 T5287 T5286 prep to,leading
R4402 T5288 T5287 pobj conversion,to
R4403 T5289 T5288 prep of,conversion
R4404 T5290 T5291 amod unmethylated,cytosine
R4405 T5291 T5289 pobj cytosine,of
R4406 T5292 T5293 aux to,uracil
R4407 T5293 T5286 xcomp uracil,leading
R4408 T5294 T5293 dobj residues,uracil
R4409 T5295 T5294 cc and,residues
R4410 T5296 T5297 det no,change
R4411 T5297 T5293 dobj change,uracil
R4412 T5298 T5297 prep of,change
R4413 T5299 T5301 amod methylated,residues
R4414 T5300 T5301 compound cytosine,residues
R4415 T5301 T5298 pobj residues,of
R4416 T5302 T5304 punct ",",performed
R4417 T5303 T5304 auxpass was,performed
R4418 T5304 T5304 ROOT performed,performed
R4419 T5305 T5306 advmod as,described
R4420 T5306 T5304 advcl described,performed
R4421 T5307 T5308 mark as,follows
R4422 T5308 T5304 advcl follows,performed
R4423 T5309 T5304 punct .,performed
R4424 T5310 T5338 advmod Briefly,denaturated
R4425 T5311 T5338 punct ",",denaturated
R4426 T5312 T5313 nummod 1,µg
R4427 T5313 T5338 nsubjpass µg,denaturated
R4428 T5314 T5313 prep of,µg
R4429 T5315 T5314 pobj DNA,of
R4430 T5316 T5313 cc and,µg
R4431 T5317 T5318 nummod 2,µg
R4432 T5318 T5313 conj µg,µg
R4433 T5319 T5318 prep of,µg
R4434 T5320 T5319 pobj poly,of
R4435 T5321 T5322 punct (,dA
R4436 T5322 T5318 appos dA,µg
R4437 T5323 T5322 appos dT,dA
R4438 T5324 T5322 punct ),dA
R4439 T5325 T5328 punct (,dA
R4440 T5326 T5328 nmod poly,dA
R4441 T5327 T5328 punct (,dA
R4442 T5328 T5322 appos dA,dA
R4443 T5329 T5322 appos dT,dA
R4444 T5330 T5322 punct ),dA
R4445 T5331 T5318 conj copolymers,µg
R4446 T5332 T5331 punct (,copolymers
R4447 T5333 T5335 compound Amersham,Biotech
R4448 T5334 T5335 compound Pharmacia,Biotech
R4449 T5335 T5331 appos Biotech,copolymers
R4450 T5336 T5331 punct ),copolymers
R4451 T5337 T5338 auxpass were,denaturated
R4452 T5338 T5338 ROOT denaturated,denaturated
R4453 T5339 T5338 prep for,denaturated
R4454 T5340 T5341 nummod 20,min
R4455 T5341 T5339 pobj min,for
R4456 T5342 T5338 prep at,denaturated
R4457 T5343 T5344 nummod 42,°
R4458 T5344 T5345 nummod °,C
R4459 T5345 T5342 pobj C,at
R4460 T5346 T5338 prep in,denaturated
R4461 T5347 T5349 nummod 0.3,NaOH
R4462 T5348 T5349 compound M,NaOH
R4463 T5349 T5346 pobj NaOH,in
R4464 T5350 T5338 prep in,denaturated
R4465 T5351 T5352 det a,volume
R4466 T5352 T5350 pobj volume,in
R4467 T5353 T5352 prep of,volume
R4468 T5354 T5355 nummod 50,µl
R4469 T5355 T5353 pobj µl,of
R4470 T5356 T5338 punct .,denaturated
R4471 T5357 T5358 amod Fresh,solutions
R4472 T5358 T5384 nsubjpass solutions,added
R4473 T5359 T5358 prep of,solutions
R4474 T5360 T5361 nummod 30,µl
R4475 T5361 T5359 pobj µl,of
R4476 T5362 T5361 prep of,µl
R4477 T5363 T5365 nummod 10,hydrochinon
R4478 T5364 T5365 compound mM,hydrochinon
R4479 T5365 T5362 pobj hydrochinon,of
R4480 T5366 T5367 punct (,Sigma
R4481 T5367 T5365 appos Sigma,hydrochinon
R4482 T5368 T5367 punct ),Sigma
R4483 T5369 T5365 cc and,hydrochinon
R4484 T5370 T5371 nummod 530,µl
R4485 T5371 T5361 appos µl,µl
R4486 T5372 T5371 prep of,µl
R4487 T5373 T5376 nummod 3,bisulfite
R4488 T5374 T5375 compound M,sodium
R4489 T5375 T5376 compound sodium,bisulfite
R4490 T5376 T5372 pobj bisulfite,of
R4491 T5377 T5376 punct (,bisulfite
R4492 T5378 T5376 appos pH,bisulfite
R4493 T5379 T5378 nummod 5.0,pH
R4494 T5380 T5358 punct ;,solutions
R4495 T5381 T5358 appos Sigma,solutions
R4496 T5382 T5358 punct ),solutions
R4497 T5383 T5384 auxpass were,added
R4498 T5384 T5384 ROOT added,added
R4499 T5385 T5384 punct ",",added
R4500 T5386 T5387 det the,solution
R4501 T5387 T5388 nsubj solution,was
R4502 T5388 T5384 conj was,added
R4503 T5389 T5390 advmod gently,mixed
R4504 T5390 T5388 acomp mixed,was
R4505 T5391 T5388 punct ",",was
R4506 T5392 T5388 prep overlayed,was
R4507 T5393 T5392 prep with,overlayed
R4508 T5394 T5395 compound mineral,oil
R4509 T5395 T5393 pobj oil,with
R4510 T5396 T5392 cc and,overlayed
R4511 T5397 T5392 conj incubated,overlayed
R4512 T5398 T5397 prep in,incubated
R4513 T5399 T5400 det the,dark
R4514 T5400 T5398 pobj dark,in
R4515 T5401 T5397 prep for,incubated
R4516 T5402 T5401 pobj 12,for
R4517 T5403 T5404 nummod 13,h
R4518 T5404 T5402 appos h,12
R4519 T5405 T5404 prep at,h
R4520 T5406 T5407 nummod 50,°
R4521 T5407 T5405 pobj °,at
R4522 T5408 T5404 npadvmod C,h
R4523 T5409 T5388 punct .,was
R4524 T5410 T5412 det The,phase
R4525 T5411 T5412 amod aqueous,phase
R4526 T5412 T5414 nsubjpass phase,recovered
R4527 T5413 T5414 auxpass was,recovered
R4528 T5414 T5414 ROOT recovered,recovered
R4529 T5415 T5414 advcl using,recovered
R4530 T5416 T5420 det the,system
R4531 T5417 T5420 compound Wizard,system
R4532 T5418 T5420 compound DNA,system
R4533 T5419 T5420 compound clean-up,system
R4534 T5420 T5415 dobj system,using
R4535 T5421 T5420 punct (,system
R4536 T5422 T5420 appos Promega,system
R4537 T5423 T5422 punct ",",Promega
R4538 T5424 T5422 conj Mannheim,Promega
R4539 T5425 T5424 punct ",",Mannheim
R4540 T5426 T5424 conj Germany,Mannheim
R4541 T5427 T5420 punct ),system
R4542 T5428 T5414 punct .,recovered
R4543 T5429 T5431 det The,DNA
R4544 T5430 T5431 amod purified,DNA
R4545 T5431 T5432 nsubj DNA,was
R4546 T5432 T5432 ROOT was,was
R4547 T5433 T5434 advmod subsequently,mixed
R4548 T5434 T5432 acomp mixed,was
R4549 T5435 T5434 prep with,mixed
R4550 T5436 T5438 nummod 1,NaOH
R4551 T5437 T5438 compound M,NaOH
R4552 T5438 T5435 pobj NaOH,with
R4553 T5439 T5434 prep to,mixed
R4554 T5440 T5442 det a,concentration
R4555 T5441 T5442 amod final,concentration
R4556 T5442 T5439 pobj concentration,to
R4557 T5443 T5442 prep of,concentration
R4558 T5444 T5445 nummod 0.3,M
R4559 T5445 T5443 pobj M,of
R4560 T5446 T5434 cc and,mixed
R4561 T5447 T5434 conj incubated,mixed
R4562 T5448 T5447 prep for,incubated
R4563 T5449 T5450 nummod 20,min
R4564 T5450 T5448 pobj min,for
R4565 T5451 T5447 prep at,incubated
R4566 T5452 T5453 nummod 37,°
R4567 T5453 T5454 nummod °,C
R4568 T5454 T5451 pobj C,at
R4569 T5455 T5456 aux to,ensure
R4570 T5456 T5447 advcl ensure,incubated
R4571 T5457 T5458 amod complete,desulfonisation
R4572 T5458 T5456 dobj desulfonisation,ensure
R4573 T5459 T5432 punct .,was
R4574 T5460 T5461 nsubj DNA,was
R4575 T5461 T5461 ROOT was,was
R4576 T5462 T5461 attr ethanol,was
R4577 T5463 T5462 acl precipitated,ethanol
R4578 T5464 T5463 prep in,precipitated
R4579 T5465 T5466 det the,presence
R4580 T5466 T5464 pobj presence,in
R4581 T5467 T5466 prep of,presence
R4582 T5468 T5469 compound 1/10,vol
R4583 T5469 T5467 pobj vol,of
R4584 T5470 T5469 prep of,vol
R4585 T5471 T5474 nummod 3,acetate
R4586 T5472 T5474 compound M,acetate
R4587 T5473 T5474 compound sodium,acetate
R4588 T5474 T5470 pobj acetate,of
R4589 T5475 T5466 punct ",",presence
R4590 T5476 T5461 conj washed,was
R4591 T5477 T5476 prep with,washed
R4592 T5478 T5479 nummod 70,%
R4593 T5479 T5480 compound %,ethanol
R4594 T5480 T5477 pobj ethanol,with
R4595 T5481 T5480 cc and,ethanol
R4596 T5482 T5476 conj resuspended,washed
R4597 T5483 T5482 prep in,resuspended
R4598 T5484 T5485 nummod 50,µl
R4599 T5485 T5483 pobj µl,in
R4600 T5486 T5485 appos H2O,µl
R4601 T5487 T5461 punct .,was
R4602 T5488 T5490 amod Subsequent,amplification
R4603 T5489 T5490 compound PCR,amplification
R4604 T5490 T5497 nsubjpass amplification,used
R4605 T5491 T5490 prep of,amplification
R4606 T5492 T5493 nummod 4,µl
R4607 T5493 T5495 nmod µl,DNA
R4608 T5494 T5495 amod bisulfite-treated,DNA
R4609 T5495 T5491 pobj DNA,of
R4610 T5496 T5497 auxpass was,used
R4611 T5497 T5497 ROOT used,used
R4612 T5498 T5497 prep for,used
R4613 T5499 T5498 pobj cloning,for
R4614 T5500 T5499 prep of,cloning
R4615 T5501 T5502 nummod two,fragments
R4616 T5502 T5500 pobj fragments,of
R4617 T5503 T5502 prep of,fragments
R4618 T5504 T5506 det the,promoter
R4619 T5505 T5506 compound IRF-4,promoter
R4620 T5506 T5503 pobj promoter,of
R4621 T5507 T5506 punct (,promoter
R4622 T5508 T5506 appos BS-I,promoter
R4623 T5509 T5508 cc and,BS-I
R4624 T5510 T5508 conj BS-II,BS-I
R4625 T5511 T5506 punct ),promoter
R4626 T5512 T5497 prep into,used
R4627 T5513 T5515 amod pCR2,vector
R4628 T5514 T5515 nummod .1,vector
R4629 T5515 T5512 pobj vector,into
R4630 T5516 T5515 prep with,vector
R4631 T5517 T5521 det the,kit
R4632 T5518 T5519 compound TOPO,TA
R4633 T5519 T5520 nsubj TA,cloning
R4634 T5520 T5521 compound cloning,kit
R4635 T5521 T5516 pobj kit,with
R4636 T5522 T5521 punct (,kit
R4637 T5523 T5521 appos Invitrogen,kit
R4638 T5524 T5521 punct ),kit
R4639 T5525 T5521 punct (,kit
R4640 T5526 T5497 xcomp see,used
R4641 T5527 T5528 compound Figure,3A
R4642 T5528 T5526 dobj 3A,see
R4643 T5529 T5526 punct ),see
R4644 T5530 T5497 punct .,used
R4645 T5531 T5532 det The,primers
R4646 T5532 T5543 nsubj primers,contain
R4647 T5533 T5532 acl used,primers
R4648 T5534 T5533 prep for,used
R4649 T5535 T5536 compound PCR,amplification
R4650 T5536 T5534 pobj amplification,for
R4651 T5537 T5536 prep of,amplification
R4652 T5538 T5539 det the,BS-I
R4653 T5539 T5537 pobj BS-I,of
R4654 T5540 T5539 cc and,BS-I
R4655 T5541 T5539 conj BS-II,BS-I
R4656 T5542 T5543 nsubj fragments,contain
R4657 T5543 T5543 ROOT contain,contain
R4658 T5544 T5547 det the,sequence
R4659 T5545 T5547 amod putative,sequence
R4660 T5546 T5547 amod altered,sequence
R4661 T5547 T5543 dobj sequence,contain
R4662 T5548 T5547 prep of,sequence
R4663 T5549 T5551 det the,strand
R4664 T5550 T5551 compound sense,strand
R4665 T5551 T5548 pobj strand,of
R4666 T5552 T5547 amod due,sequence
R4667 T5553 T5554 aux to,bisulfite
R4668 T5554 T5552 xcomp bisulfite,due
R4669 T5555 T5554 dobj treatment,bisulfite
R4670 T5556 T5561 punct (,written
R4671 T5557 T5559 amod converted,residues
R4672 T5558 T5559 compound cytosine,residues
R4673 T5559 T5561 nsubjpass residues,written
R4674 T5560 T5561 auxpass are,written
R4675 T5561 T5543 parataxis written,contain
R4676 T5562 T5561 prep in,written
R4677 T5563 T5564 amod bold,letters
R4678 T5564 T5562 pobj letters,in
R4679 T5565 T5561 punct ),written
R4680 T5566 T5561 punct :,written
R4681 T5567 T5572 nmod BS-I-forward,′
R4682 T5568 T5567 nummod 5,BS-I-forward
R4683 T5569 T5571 punct ′,TATTTGGATTTTTAGGGAGTTTTTTTT-3
R4684 T5570 T5571 punct -,TATTTGGATTTTTAGGGAGTTTTTTTT-3
R4685 T5571 T5572 compound TATTTGGATTTTTAGGGAGTTTTTTTT-3,′
R4686 T5572 T5561 dep ′,written
R4687 T5573 T5572 punct ",",′
R4688 T5574 T5578 dep BS-I-reverse,ACCCAACTCCCTTAAACTATTAAACT-3
R4689 T5575 T5574 nummod 5,BS-I-reverse
R4690 T5576 T5578 punct ′,ACCCAACTCCCTTAAACTATTAAACT-3
R4691 T5577 T5578 punct -,ACCCAACTCCCTTAAACTATTAAACT-3
R4692 T5578 T5579 compound ACCCAACTCCCTTAAACTATTAAACT-3,′
R4693 T5579 T5572 appos ′,′
R4694 T5580 T5579 punct (,′
R4695 T5581 T5582 nummod 187,bp
R4696 T5582 T5579 appos bp,′
R4697 T5583 T5579 punct ),′
R4698 T5584 T5572 punct ;,′
R4699 T5585 T5590 nmod BS-II-forward,′
R4700 T5586 T5585 nummod 5,BS-II-forward
R4701 T5587 T5589 punct ′,AGTTTAATAGTTTAAGGGAGTTGGGT-3
R4702 T5588 T5589 punct -,AGTTTAATAGTTTAAGGGAGTTGGGT-3
R4703 T5589 T5590 compound AGTTTAATAGTTTAAGGGAGTTGGGT-3,′
R4704 T5590 T5572 appos ′,′
R4705 T5591 T5590 punct ",",′
R4706 T5592 T5596 dep BS-II-reverse,CTCACCCTAAACTCAAAACTAAAAAC-3
R4707 T5593 T5592 nummod 5,BS-II-reverse
R4708 T5594 T5596 punct ′,CTCACCCTAAACTCAAAACTAAAAAC-3
R4709 T5595 T5596 punct -,CTCACCCTAAACTCAAAACTAAAAAC-3
R4710 T5596 T5597 compound CTCACCCTAAACTCAAAACTAAAAAC-3,′
R4711 T5597 T5590 appos ′,′
R4712 T5598 T5597 punct (,′
R4713 T5599 T5600 nummod 674,bp
R4714 T5600 T5597 appos bp,′
R4715 T5601 T5597 punct ),′
R4716 T5602 T5543 punct .,contain
R4717 T5603 T5621 prep After,sequenced
R4718 T5604 T5605 amod bacterial,amplification
R4719 T5605 T5603 pobj amplification,After
R4720 T5606 T5605 prep of,amplification
R4721 T5607 T5610 det the,fragments
R4722 T5608 T5610 amod cloned,fragments
R4723 T5609 T5610 compound PCR,fragments
R4724 T5610 T5606 pobj fragments,of
R4725 T5611 T5605 prep by,amplification
R4726 T5612 T5613 amod standard,procedures
R4727 T5613 T5611 pobj procedures,by
R4728 T5614 T5621 punct ",",sequenced
R4729 T5615 T5616 nummod eight,clones
R4730 T5616 T5621 nsubjpass clones,sequenced
R4731 T5617 T5616 prep from,clones
R4732 T5618 T5619 det each,sample
R4733 T5619 T5617 pobj sample,from
R4734 T5620 T5621 auxpass were,sequenced
R4735 T5621 T5621 ROOT sequenced,sequenced
R4736 T5622 T5621 prep with,sequenced
R4737 T5623 T5625 det an,sequencer
R4738 T5624 T5625 amod automated,sequencer
R4739 T5625 T5622 pobj sequencer,with
R4740 T5626 T5625 punct (,sequencer
R4741 T5627 T5628 compound ABI,Prism
R4742 T5628 T5625 appos Prism,sequencer
R4743 T5629 T5628 nummod 377,Prism
R4744 T5630 T5628 punct ",",Prism
R4745 T5631 T5632 compound Applied,Biosystems
R4746 T5632 T5628 appos Biosystems,Prism
R4747 T5633 T5625 punct ),sequencer
R4748 T5634 T5621 punct .,sequenced
R4926 T5873 T5887 prep In,provided
R4927 T5874 T5875 amod vitro,methylation
R4928 T5875 T5873 pobj methylation,In
R4929 T5876 T5875 cc and,methylation
R4930 T5877 T5878 compound reporter,gene
R4931 T5878 T5879 compound gene,assays
R4932 T5879 T5884 nsubj assays,construct
R4933 T5880 T5883 det The,gene
R4934 T5881 T5883 amod IRF-4,gene
R4935 T5882 T5883 compound promoter-reporter,gene
R4936 T5883 T5884 compound gene,construct
R4937 T5884 T5887 csubjpass construct,provided
R4938 T5885 T5887 auxpass was,provided
R4939 T5886 T5887 advmod generously,provided
R4940 T5887 T5887 ROOT provided,provided
R4941 T5888 T5887 agent by,provided
R4942 T5889 T5890 compound J.,Hiscott
R4943 T5890 T5888 pobj Hiscott,by
R4944 T5891 T5890 punct (,Hiscott
R4945 T5892 T5890 appos 31,Hiscott
R4946 T5893 T5890 punct ),Hiscott
R4947 T5894 T5887 punct .,provided
R4948 T5895 T5897 nsubjpass Constructs,methylated
R4949 T5896 T5897 auxpass were,methylated
R4950 T5897 T5897 ROOT methylated,methylated
R4951 T5898 T5897 prep in,methylated
R4952 T5899 T5898 pobj vitro,in
R4953 T5900 T5897 prep with,methylated
R4954 T5901 T5902 compound CpG,Methylase
R4955 T5902 T5900 pobj Methylase,with
R4956 T5903 T5904 punct (,M.Sss
R4957 T5904 T5902 appos M.Sss,Methylase
R4958 T5905 T5904 appos I,M.Sss
R4959 T5906 T5904 punct ),M.Sss
R4960 T5907 T5908 mark as,recommended
R4961 T5908 T5897 advcl recommended,methylated
R4962 T5909 T5908 agent by,recommended
R4963 T5910 T5911 det the,manufacturer
R4964 T5911 T5909 pobj manufacturer,by
R4965 T5912 T5914 punct (,Biolabs
R4966 T5913 T5914 compound NE,Biolabs
R4967 T5914 T5911 appos Biolabs,manufacturer
R4968 T5915 T5914 punct ),Biolabs
R4969 T5916 T5908 cc and,recommended
R4970 T5917 T5918 amod complete,methylation
R4971 T5918 T5920 nsubjpass methylation,checked
R4972 T5919 T5920 auxpass was,checked
R4973 T5920 T5897 conj checked,methylated
R4974 T5921 T5920 prep via,checked
R4975 T5922 T5923 compound restriction,analysis
R4976 T5923 T5921 pobj analysis,via
R4977 T5924 T5926 punct (,5A
R4978 T5925 T5926 nmod Figure,5A
R4979 T5926 T5923 appos 5A,analysis
R4980 T5927 T5926 punct ),5A
R4981 T5928 T5920 punct .,checked
R4982 T5929 T5931 compound Reporter,assays
R4983 T5930 T5931 compound gene,assays
R4984 T5931 T5941 nsubjpass assays,performed
R4985 T5932 T5931 acl using,assays
R4986 T5933 T5936 det the,assay
R4987 T5934 T5935 amod dual,luciferase
R4988 T5935 T5936 compound luciferase,assay
R4989 T5936 T5932 dobj assay,using
R4990 T5937 T5936 punct (,assay
R4991 T5938 T5936 appos Promega,assay
R4992 T5939 T5936 punct ),assay
R4993 T5940 T5941 auxpass were,performed
R4994 T5941 T5941 ROOT performed,performed
R4995 T5942 T5941 oprd similar,performed
R4996 T5943 T5942 prep to,similar
R4997 T5944 T5945 amod previous,reports
R4998 T5945 T5943 pobj reports,to
R4999 T5946 T5947 punct (,29
R5000 T5947 T5945 appos 29,reports
R5001 T5948 T5941 punct ),performed
R5002 T5949 T5941 punct .,performed
R5003 T5950 T5957 advmod Briefly,construct
R5004 T5951 T5957 punct ",",construct
R5005 T5952 T5953 nummod 5,nM
R5006 T5953 T5957 nsubj nM,construct
R5007 T5954 T5953 prep of,nM
R5008 T5955 T5956 det the,reporter
R5009 T5956 T5954 pobj reporter,of
R5010 T5957 T5957 ROOT construct,construct
R5011 T5958 T5957 cc and,construct
R5012 T5959 T5961 det the,control
R5013 T5960 T5961 compound transfection,control
R5014 T5961 T5969 nsubjpass control,transientlyco-expressed
R5015 T5962 T5969 csubjpass construct,transientlyco-expressed
R5016 T5963 T5962 xcomp expressing,construct
R5017 T5964 T5967 det the,gene
R5018 T5965 T5967 nmod renilla,gene
R5019 T5966 T5967 compound luciferase,gene
R5020 T5967 T5963 dobj gene,expressing
R5021 T5968 T5969 auxpass were,transientlyco-expressed
R5022 T5969 T5957 conj transientlyco-expressed,construct
R5023 T5970 T5969 prep via,transientlyco-expressed
R5024 T5971 T5970 pobj electroporation,via
R5025 T5972 T5957 punct .,construct
R5026 T5973 T5974 det The,control
R5027 T5974 T5976 nsubj control,served
R5028 T5975 T5976 nsubj construct,served
R5029 T5976 T5976 ROOT served,served
R5030 T5977 T5976 prep as,served
R5031 T5978 T5980 det an,reference
R5032 T5979 T5980 amod internal,reference
R5033 T5980 T5977 pobj reference,as
R5034 T5981 T5980 prep for,reference
R5035 T5982 T5983 compound transfection,efficiency
R5036 T5983 T5981 pobj efficiency,for
R5037 T5984 T5976 punct .,served
R5038 T5985 T5986 amod Forty-eight,hours
R5039 T5986 T5987 npadvmod hours,after
R5040 T5987 T5993 prep after,measured
R5041 T5988 T5987 pobj transfection,after
R5042 T5989 T5993 punct ",",measured
R5043 T5990 T5991 compound luciferase,activity
R5044 T5991 T5993 nsubjpass activity,measured
R5045 T5992 T5993 auxpass was,measured
R5046 T5993 T5993 ROOT measured,measured
R5047 T5994 T5993 prep with,measured
R5048 T5995 T5999 det a,microlumat
R5049 T5996 T5999 nmod LB,microlumat
R5050 T5997 T5999 nummod 96,microlumat
R5051 T5998 T5999 compound P,microlumat
R5052 T5999 T5994 pobj microlumat,with
R5053 T6000 T5999 punct (,microlumat
R5054 T6001 T6002 compound EG&G,Berthold
R5055 T6002 T5999 appos Berthold,microlumat
R5056 T6003 T6002 punct ",",Berthold
R5057 T6004 T6005 compound Bad,Wildbad
R5058 T6005 T6002 appos Wildbad,Berthold
R5059 T6006 T6005 punct ",",Wildbad
R5060 T6007 T6005 appos Germany,Wildbad
R5061 T6008 T6005 punct ),Wildbad
R5062 T6009 T5993 punct .,measured
R5063 T6010 T6012 nummod IRF-4,activation
R5064 T6011 T6012 compound promoter,activation
R5065 T6012 T6014 nsubjpass activation,quantified
R5066 T6013 T6014 auxpass was,quantified
R5067 T6014 T6014 ROOT quantified,quantified
R5068 T6015 T6014 prep as,quantified
R5069 T6016 T6017 det a,ratio
R5070 T6017 T6015 pobj ratio,as
R5071 T6018 T6017 prep of,ratio
R5072 T6019 T6022 amod measured,units
R5073 T6020 T6021 advmod firefly,light
R5074 T6021 T6022 amod light,units
R5075 T6022 T6018 pobj units,of
R5076 T6023 T6022 punct (,units
R5077 T6024 T6022 appos flu,units
R5078 T6025 T6022 punct ),units
R5079 T6026 T6017 amod relative,ratio
R5080 T6027 T6026 prep to,relative
R5081 T6028 T6027 pobj renilla,to
R5082 T6029 T6030 punct (,rlu
R5083 T6030 T6028 appos rlu,renilla
R5084 T6031 T6030 punct ),rlu
R5085 T6032 T6014 punct .,quantified
R5086 T6033 T6034 det Each,experiment
R5087 T6034 T6036 nsubjpass experiment,carried
R5088 T6035 T6036 auxpass was,carried
R5089 T6036 T6036 ROOT carried,carried
R5090 T6037 T6036 prt out,carried
R5091 T6038 T6039 advmod at,least
R5092 T6039 T6040 advmod least,three
R5093 T6040 T6041 nummod three,times
R5094 T6041 T6036 npadvmod times,carried
R5095 T6042 T6036 punct .,carried