Id |
Subject |
Object |
Predicate |
Lexical cue |
T5873 |
0-2 |
IN |
denotes |
In |
T5874 |
3-8 |
NN |
denotes |
vitro |
T5875 |
9-20 |
NN |
denotes |
methylation |
T5876 |
21-24 |
CC |
denotes |
and |
T5877 |
25-33 |
NN |
denotes |
reporter |
T5878 |
34-38 |
NN |
denotes |
gene |
T5879 |
39-45 |
VBZ |
denotes |
assays |
T5880 |
46-49 |
DT |
denotes |
The |
T5881 |
50-55 |
JJ |
denotes |
IRF-4 |
T5882 |
56-73 |
NN |
denotes |
promoter-reporter |
T5883 |
74-78 |
NN |
denotes |
gene |
T5884 |
79-88 |
VBP |
denotes |
construct |
T5885 |
89-92 |
VBD |
denotes |
was |
T5886 |
93-103 |
RB |
denotes |
generously |
T5887 |
104-112 |
VBN |
denotes |
provided |
T5888 |
113-115 |
IN |
denotes |
by |
T5889 |
116-118 |
NNP |
denotes |
J. |
T5890 |
119-126 |
NNP |
denotes |
Hiscott |
T5891 |
127-128 |
-LRB- |
denotes |
( |
T5892 |
128-130 |
CD |
denotes |
31 |
T5893 |
130-131 |
-RRB- |
denotes |
) |
T5894 |
131-132 |
. |
denotes |
. |
T5895 |
133-143 |
NNS |
denotes |
Constructs |
T5896 |
144-148 |
VBD |
denotes |
were |
T5897 |
149-159 |
VBN |
denotes |
methylated |
T5898 |
160-162 |
IN |
denotes |
in |
T5899 |
163-168 |
NN |
denotes |
vitro |
T5900 |
169-173 |
IN |
denotes |
with |
T5901 |
174-177 |
NNP |
denotes |
CpG |
T5902 |
178-187 |
NNP |
denotes |
Methylase |
T5903 |
188-189 |
-LRB- |
denotes |
( |
T5904 |
189-194 |
NNP |
denotes |
M.Sss |
T5905 |
195-196 |
PRP |
denotes |
I |
T5906 |
196-197 |
-RRB- |
denotes |
) |
T5907 |
198-200 |
IN |
denotes |
as |
T5908 |
201-212 |
VBN |
denotes |
recommended |
T5909 |
213-215 |
IN |
denotes |
by |
T5910 |
216-219 |
DT |
denotes |
the |
T5911 |
220-232 |
NN |
denotes |
manufacturer |
T5912 |
233-234 |
-LRB- |
denotes |
( |
T5913 |
234-236 |
NNP |
denotes |
NE |
T5914 |
237-244 |
NNPS |
denotes |
Biolabs |
T5915 |
244-245 |
-RRB- |
denotes |
) |
T5916 |
246-249 |
CC |
denotes |
and |
T5917 |
250-258 |
JJ |
denotes |
complete |
T5918 |
259-270 |
NN |
denotes |
methylation |
T5919 |
271-274 |
VBD |
denotes |
was |
T5920 |
275-282 |
VBN |
denotes |
checked |
T5921 |
283-286 |
IN |
denotes |
via |
T5922 |
287-298 |
NN |
denotes |
restriction |
T5923 |
299-307 |
NN |
denotes |
analysis |
T5924 |
308-309 |
-LRB- |
denotes |
( |
T5925 |
309-315 |
NN |
denotes |
Figure |
T5926 |
316-318 |
NN |
denotes |
5A |
T5927 |
318-319 |
-RRB- |
denotes |
) |
T5928 |
319-320 |
. |
denotes |
. |
T5929 |
321-329 |
NN |
denotes |
Reporter |
T5930 |
330-334 |
NN |
denotes |
gene |
T5931 |
335-341 |
NNS |
denotes |
assays |
T5932 |
342-347 |
VBG |
denotes |
using |
T5933 |
348-351 |
DT |
denotes |
the |
T5934 |
352-356 |
JJ |
denotes |
dual |
T5935 |
357-367 |
NN |
denotes |
luciferase |
T5936 |
368-373 |
NN |
denotes |
assay |
T5937 |
374-375 |
-LRB- |
denotes |
( |
T5938 |
375-382 |
NNP |
denotes |
Promega |
T5939 |
382-383 |
-RRB- |
denotes |
) |
T5940 |
384-388 |
VBD |
denotes |
were |
T5941 |
389-398 |
VBN |
denotes |
performed |
T5942 |
399-406 |
JJ |
denotes |
similar |
T5943 |
407-409 |
TO |
denotes |
to |
T5944 |
410-418 |
JJ |
denotes |
previous |
T5945 |
419-426 |
NNS |
denotes |
reports |
T5946 |
427-428 |
-LRB- |
denotes |
( |
T5947 |
428-430 |
CD |
denotes |
29 |
T5948 |
430-431 |
-RRB- |
denotes |
) |
T5949 |
431-432 |
. |
denotes |
. |
T5950 |
433-440 |
RB |
denotes |
Briefly |
T5951 |
440-441 |
, |
denotes |
, |
T5952 |
442-443 |
CD |
denotes |
5 |
T5953 |
444-446 |
NN |
denotes |
nM |
T5954 |
447-449 |
IN |
denotes |
of |
T5955 |
450-453 |
DT |
denotes |
the |
T5956 |
454-462 |
NN |
denotes |
reporter |
T5957 |
463-472 |
VB |
denotes |
construct |
T5958 |
473-476 |
CC |
denotes |
and |
T5959 |
477-480 |
DT |
denotes |
the |
T5960 |
481-493 |
NN |
denotes |
transfection |
T5961 |
494-501 |
NN |
denotes |
control |
T5962 |
502-511 |
VBP |
denotes |
construct |
T5963 |
512-522 |
VBG |
denotes |
expressing |
T5964 |
523-526 |
DT |
denotes |
the |
T5965 |
527-534 |
NN |
denotes |
renilla |
T5966 |
535-545 |
NN |
denotes |
luciferase |
T5967 |
546-550 |
NN |
denotes |
gene |
T5968 |
551-555 |
VBD |
denotes |
were |
T5969 |
556-579 |
JJ |
denotes |
transientlyco-expressed |
T5970 |
580-583 |
IN |
denotes |
via |
T5971 |
584-599 |
NN |
denotes |
electroporation |
T5972 |
599-600 |
. |
denotes |
. |
T5973 |
601-604 |
DT |
denotes |
The |
T5974 |
605-612 |
NN |
denotes |
control |
T5975 |
613-622 |
VBP |
denotes |
construct |
T5976 |
623-629 |
VBN |
denotes |
served |
T5977 |
630-632 |
IN |
denotes |
as |
T5978 |
633-635 |
DT |
denotes |
an |
T5979 |
636-644 |
JJ |
denotes |
internal |
T5980 |
645-654 |
NN |
denotes |
reference |
T5981 |
655-658 |
IN |
denotes |
for |
T5982 |
659-671 |
NN |
denotes |
transfection |
T5983 |
672-682 |
NN |
denotes |
efficiency |
T5984 |
682-683 |
. |
denotes |
. |
T5985 |
684-695 |
JJ |
denotes |
Forty-eight |
T5986 |
696-701 |
NNS |
denotes |
hours |
T5987 |
702-707 |
IN |
denotes |
after |
T5988 |
708-720 |
NN |
denotes |
transfection |
T5989 |
720-721 |
, |
denotes |
, |
T5990 |
722-732 |
NN |
denotes |
luciferase |
T5991 |
733-741 |
NN |
denotes |
activity |
T5992 |
742-745 |
VBD |
denotes |
was |
T5993 |
746-754 |
VBN |
denotes |
measured |
T5994 |
755-759 |
IN |
denotes |
with |
T5995 |
760-761 |
DT |
denotes |
a |
T5996 |
762-764 |
NNP |
denotes |
LB |
T5997 |
765-767 |
CD |
denotes |
96 |
T5998 |
768-769 |
NNP |
denotes |
P |
T5999 |
770-780 |
NN |
denotes |
microlumat |
T6000 |
781-782 |
-LRB- |
denotes |
( |
T6001 |
782-786 |
NNP |
denotes |
EG&G |
T6002 |
787-795 |
NNP |
denotes |
Berthold |
T6003 |
795-796 |
, |
denotes |
, |
T6004 |
797-800 |
NNP |
denotes |
Bad |
T6005 |
801-808 |
NNP |
denotes |
Wildbad |
T6006 |
808-809 |
, |
denotes |
, |
T6007 |
810-817 |
NNP |
denotes |
Germany |
T6008 |
817-818 |
-RRB- |
denotes |
) |
T6009 |
818-819 |
. |
denotes |
. |
T6010 |
820-825 |
CD |
denotes |
IRF-4 |
T6011 |
826-834 |
NN |
denotes |
promoter |
T6012 |
835-845 |
NN |
denotes |
activation |
T6013 |
846-849 |
VBD |
denotes |
was |
T6014 |
850-860 |
VBN |
denotes |
quantified |
T6015 |
861-863 |
IN |
denotes |
as |
T6016 |
864-865 |
DT |
denotes |
a |
T6017 |
866-871 |
NN |
denotes |
ratio |
T6018 |
872-874 |
IN |
denotes |
of |
T6019 |
875-883 |
VBN |
denotes |
measured |
T6020 |
884-891 |
RB |
denotes |
firefly |
T6021 |
892-897 |
JJ |
denotes |
light |
T6022 |
898-903 |
NNS |
denotes |
units |
T6023 |
904-905 |
-LRB- |
denotes |
( |
T6024 |
905-908 |
NN |
denotes |
flu |
T6025 |
908-909 |
-RRB- |
denotes |
) |
T6026 |
910-918 |
JJ |
denotes |
relative |
T6027 |
919-921 |
TO |
denotes |
to |
T6028 |
922-929 |
NN |
denotes |
renilla |
T6029 |
930-931 |
-LRB- |
denotes |
( |
T6030 |
931-934 |
NN |
denotes |
rlu |
T6031 |
934-935 |
-RRB- |
denotes |
) |
T6032 |
935-936 |
. |
denotes |
. |
T6033 |
937-941 |
DT |
denotes |
Each |
T6034 |
942-952 |
NN |
denotes |
experiment |
T6035 |
953-956 |
VBD |
denotes |
was |
T6036 |
957-964 |
VBN |
denotes |
carried |
T6037 |
965-968 |
RP |
denotes |
out |
T6038 |
969-971 |
IN |
denotes |
at |
T6039 |
972-977 |
JJS |
denotes |
least |
T6040 |
978-983 |
CD |
denotes |
three |
T6041 |
984-989 |
NNS |
denotes |
times |
T6042 |
989-990 |
. |
denotes |
. |
R4926 |
T5873 |
T5887 |
prep |
In,provided |
R4927 |
T5874 |
T5875 |
amod |
vitro,methylation |
R4928 |
T5875 |
T5873 |
pobj |
methylation,In |
R4929 |
T5876 |
T5875 |
cc |
and,methylation |
R4930 |
T5877 |
T5878 |
compound |
reporter,gene |
R4931 |
T5878 |
T5879 |
compound |
gene,assays |
R4932 |
T5879 |
T5884 |
nsubj |
assays,construct |
R4933 |
T5880 |
T5883 |
det |
The,gene |
R4934 |
T5881 |
T5883 |
amod |
IRF-4,gene |
R4935 |
T5882 |
T5883 |
compound |
promoter-reporter,gene |
R4936 |
T5883 |
T5884 |
compound |
gene,construct |
R4937 |
T5884 |
T5887 |
csubjpass |
construct,provided |
R4938 |
T5885 |
T5887 |
auxpass |
was,provided |
R4939 |
T5886 |
T5887 |
advmod |
generously,provided |
R4940 |
T5887 |
T5887 |
ROOT |
provided,provided |
R4941 |
T5888 |
T5887 |
agent |
by,provided |
R4942 |
T5889 |
T5890 |
compound |
J.,Hiscott |
R4943 |
T5890 |
T5888 |
pobj |
Hiscott,by |
R4944 |
T5891 |
T5890 |
punct |
(,Hiscott |
R4945 |
T5892 |
T5890 |
appos |
31,Hiscott |
R4946 |
T5893 |
T5890 |
punct |
),Hiscott |
R4947 |
T5894 |
T5887 |
punct |
.,provided |
R4948 |
T5895 |
T5897 |
nsubjpass |
Constructs,methylated |
R4949 |
T5896 |
T5897 |
auxpass |
were,methylated |
R4950 |
T5897 |
T5897 |
ROOT |
methylated,methylated |
R4951 |
T5898 |
T5897 |
prep |
in,methylated |
R4952 |
T5899 |
T5898 |
pobj |
vitro,in |
R4953 |
T5900 |
T5897 |
prep |
with,methylated |
R4954 |
T5901 |
T5902 |
compound |
CpG,Methylase |
R4955 |
T5902 |
T5900 |
pobj |
Methylase,with |
R4956 |
T5903 |
T5904 |
punct |
(,M.Sss |
R4957 |
T5904 |
T5902 |
appos |
M.Sss,Methylase |
R4958 |
T5905 |
T5904 |
appos |
I,M.Sss |
R4959 |
T5906 |
T5904 |
punct |
),M.Sss |
R4960 |
T5907 |
T5908 |
mark |
as,recommended |
R4961 |
T5908 |
T5897 |
advcl |
recommended,methylated |
R4962 |
T5909 |
T5908 |
agent |
by,recommended |
R4963 |
T5910 |
T5911 |
det |
the,manufacturer |
R4964 |
T5911 |
T5909 |
pobj |
manufacturer,by |
R4965 |
T5912 |
T5914 |
punct |
(,Biolabs |
R4966 |
T5913 |
T5914 |
compound |
NE,Biolabs |
R4967 |
T5914 |
T5911 |
appos |
Biolabs,manufacturer |
R4968 |
T5915 |
T5914 |
punct |
),Biolabs |
R4969 |
T5916 |
T5908 |
cc |
and,recommended |
R4970 |
T5917 |
T5918 |
amod |
complete,methylation |
R4971 |
T5918 |
T5920 |
nsubjpass |
methylation,checked |
R4972 |
T5919 |
T5920 |
auxpass |
was,checked |
R4973 |
T5920 |
T5897 |
conj |
checked,methylated |
R4974 |
T5921 |
T5920 |
prep |
via,checked |
R4975 |
T5922 |
T5923 |
compound |
restriction,analysis |
R4976 |
T5923 |
T5921 |
pobj |
analysis,via |
R4977 |
T5924 |
T5926 |
punct |
(,5A |
R4978 |
T5925 |
T5926 |
nmod |
Figure,5A |
R4979 |
T5926 |
T5923 |
appos |
5A,analysis |
R4980 |
T5927 |
T5926 |
punct |
),5A |
R4981 |
T5928 |
T5920 |
punct |
.,checked |
R4982 |
T5929 |
T5931 |
compound |
Reporter,assays |
R4983 |
T5930 |
T5931 |
compound |
gene,assays |
R4984 |
T5931 |
T5941 |
nsubjpass |
assays,performed |
R4985 |
T5932 |
T5931 |
acl |
using,assays |
R4986 |
T5933 |
T5936 |
det |
the,assay |
R4987 |
T5934 |
T5935 |
amod |
dual,luciferase |
R4988 |
T5935 |
T5936 |
compound |
luciferase,assay |
R4989 |
T5936 |
T5932 |
dobj |
assay,using |
R4990 |
T5937 |
T5936 |
punct |
(,assay |
R4991 |
T5938 |
T5936 |
appos |
Promega,assay |
R4992 |
T5939 |
T5936 |
punct |
),assay |
R4993 |
T5940 |
T5941 |
auxpass |
were,performed |
R4994 |
T5941 |
T5941 |
ROOT |
performed,performed |
R4995 |
T5942 |
T5941 |
oprd |
similar,performed |
R4996 |
T5943 |
T5942 |
prep |
to,similar |
R4997 |
T5944 |
T5945 |
amod |
previous,reports |
R4998 |
T5945 |
T5943 |
pobj |
reports,to |
R4999 |
T5946 |
T5947 |
punct |
(,29 |
R5000 |
T5947 |
T5945 |
appos |
29,reports |
R5001 |
T5948 |
T5941 |
punct |
),performed |
R5002 |
T5949 |
T5941 |
punct |
.,performed |
R5003 |
T5950 |
T5957 |
advmod |
Briefly,construct |
R5004 |
T5951 |
T5957 |
punct |
",",construct |
R5005 |
T5952 |
T5953 |
nummod |
5,nM |
R5006 |
T5953 |
T5957 |
nsubj |
nM,construct |
R5007 |
T5954 |
T5953 |
prep |
of,nM |
R5008 |
T5955 |
T5956 |
det |
the,reporter |
R5009 |
T5956 |
T5954 |
pobj |
reporter,of |
R5010 |
T5957 |
T5957 |
ROOT |
construct,construct |
R5011 |
T5958 |
T5957 |
cc |
and,construct |
R5012 |
T5959 |
T5961 |
det |
the,control |
R5013 |
T5960 |
T5961 |
compound |
transfection,control |
R5014 |
T5961 |
T5969 |
nsubjpass |
control,transientlyco-expressed |
R5015 |
T5962 |
T5969 |
csubjpass |
construct,transientlyco-expressed |
R5016 |
T5963 |
T5962 |
xcomp |
expressing,construct |
R5017 |
T5964 |
T5967 |
det |
the,gene |
R5018 |
T5965 |
T5967 |
nmod |
renilla,gene |
R5019 |
T5966 |
T5967 |
compound |
luciferase,gene |
R5020 |
T5967 |
T5963 |
dobj |
gene,expressing |
R5021 |
T5968 |
T5969 |
auxpass |
were,transientlyco-expressed |
R5022 |
T5969 |
T5957 |
conj |
transientlyco-expressed,construct |
R5023 |
T5970 |
T5969 |
prep |
via,transientlyco-expressed |
R5024 |
T5971 |
T5970 |
pobj |
electroporation,via |
R5025 |
T5972 |
T5957 |
punct |
.,construct |
R5026 |
T5973 |
T5974 |
det |
The,control |
R5027 |
T5974 |
T5976 |
nsubj |
control,served |
R5028 |
T5975 |
T5976 |
nsubj |
construct,served |
R5029 |
T5976 |
T5976 |
ROOT |
served,served |
R5030 |
T5977 |
T5976 |
prep |
as,served |
R5031 |
T5978 |
T5980 |
det |
an,reference |
R5032 |
T5979 |
T5980 |
amod |
internal,reference |
R5033 |
T5980 |
T5977 |
pobj |
reference,as |
R5034 |
T5981 |
T5980 |
prep |
for,reference |
R5035 |
T5982 |
T5983 |
compound |
transfection,efficiency |
R5036 |
T5983 |
T5981 |
pobj |
efficiency,for |
R5037 |
T5984 |
T5976 |
punct |
.,served |
R5038 |
T5985 |
T5986 |
amod |
Forty-eight,hours |
R5039 |
T5986 |
T5987 |
npadvmod |
hours,after |
R5040 |
T5987 |
T5993 |
prep |
after,measured |
R5041 |
T5988 |
T5987 |
pobj |
transfection,after |
R5042 |
T5989 |
T5993 |
punct |
",",measured |
R5043 |
T5990 |
T5991 |
compound |
luciferase,activity |
R5044 |
T5991 |
T5993 |
nsubjpass |
activity,measured |
R5045 |
T5992 |
T5993 |
auxpass |
was,measured |
R5046 |
T5993 |
T5993 |
ROOT |
measured,measured |
R5047 |
T5994 |
T5993 |
prep |
with,measured |
R5048 |
T5995 |
T5999 |
det |
a,microlumat |
R5049 |
T5996 |
T5999 |
nmod |
LB,microlumat |
R5050 |
T5997 |
T5999 |
nummod |
96,microlumat |
R5051 |
T5998 |
T5999 |
compound |
P,microlumat |
R5052 |
T5999 |
T5994 |
pobj |
microlumat,with |
R5053 |
T6000 |
T5999 |
punct |
(,microlumat |
R5054 |
T6001 |
T6002 |
compound |
EG&G,Berthold |
R5055 |
T6002 |
T5999 |
appos |
Berthold,microlumat |
R5056 |
T6003 |
T6002 |
punct |
",",Berthold |
R5057 |
T6004 |
T6005 |
compound |
Bad,Wildbad |
R5058 |
T6005 |
T6002 |
appos |
Wildbad,Berthold |
R5059 |
T6006 |
T6005 |
punct |
",",Wildbad |
R5060 |
T6007 |
T6005 |
appos |
Germany,Wildbad |
R5061 |
T6008 |
T6005 |
punct |
),Wildbad |
R5062 |
T6009 |
T5993 |
punct |
.,measured |
R5063 |
T6010 |
T6012 |
nummod |
IRF-4,activation |
R5064 |
T6011 |
T6012 |
compound |
promoter,activation |
R5065 |
T6012 |
T6014 |
nsubjpass |
activation,quantified |
R5066 |
T6013 |
T6014 |
auxpass |
was,quantified |
R5067 |
T6014 |
T6014 |
ROOT |
quantified,quantified |
R5068 |
T6015 |
T6014 |
prep |
as,quantified |
R5069 |
T6016 |
T6017 |
det |
a,ratio |
R5070 |
T6017 |
T6015 |
pobj |
ratio,as |
R5071 |
T6018 |
T6017 |
prep |
of,ratio |
R5072 |
T6019 |
T6022 |
amod |
measured,units |
R5073 |
T6020 |
T6021 |
advmod |
firefly,light |
R5074 |
T6021 |
T6022 |
amod |
light,units |
R5075 |
T6022 |
T6018 |
pobj |
units,of |
R5076 |
T6023 |
T6022 |
punct |
(,units |
R5077 |
T6024 |
T6022 |
appos |
flu,units |
R5078 |
T6025 |
T6022 |
punct |
),units |
R5079 |
T6026 |
T6017 |
amod |
relative,ratio |
R5080 |
T6027 |
T6026 |
prep |
to,relative |
R5081 |
T6028 |
T6027 |
pobj |
renilla,to |
R5082 |
T6029 |
T6030 |
punct |
(,rlu |
R5083 |
T6030 |
T6028 |
appos |
rlu,renilla |
R5084 |
T6031 |
T6030 |
punct |
),rlu |
R5085 |
T6032 |
T6014 |
punct |
.,quantified |
R5086 |
T6033 |
T6034 |
det |
Each,experiment |
R5087 |
T6034 |
T6036 |
nsubjpass |
experiment,carried |
R5088 |
T6035 |
T6036 |
auxpass |
was,carried |
R5089 |
T6036 |
T6036 |
ROOT |
carried,carried |
R5090 |
T6037 |
T6036 |
prt |
out,carried |
R5091 |
T6038 |
T6039 |
advmod |
at,least |
R5092 |
T6039 |
T6040 |
advmod |
least,three |
R5093 |
T6040 |
T6041 |
nummod |
three,times |
R5094 |
T6041 |
T6036 |
npadvmod |
times,carried |
R5095 |
T6042 |
T6036 |
punct |
.,carried |