PMC:1064895 / 9463-10900 24 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T7848 | 0-5 | NNP | denotes | IL-17 |
| T7849 | 6-8 | IN | denotes | in |
| T7850 | 9-16 | NN | denotes | culture |
| T7851 | 17-29 | NNS | denotes | supernatants |
| T7852 | 30-33 | VBD | denotes | was |
| T7853 | 34-42 | VBN | denotes | measured |
| T7854 | 43-45 | IN | denotes | by |
| T7855 | 46-54 | NN | denotes | sandwich |
| T7856 | 55-68 | JJ | denotes | enzyme-linked |
| T7857 | 69-82 | JJ | denotes | immunosorbent |
| T7858 | 83-88 | NN | denotes | assay |
| T7859 | 89-91 | IN | denotes | as |
| T7860 | 92-101 | VBN | denotes | described |
| T7861 | 102-112 | RB | denotes | previously |
| T7862 | 113-114 | NNP | denotes | [ |
| T7863 | 114-116 | CD | denotes | 20 |
| T7864 | 116-117 | NNP | denotes | ] |
| T7865 | 117-118 | . | denotes | . |
| T7866 | 119-121 | IN | denotes | In |
| T7867 | 122-127 | NN | denotes | brief |
| T7868 | 127-128 | , | denotes | , |
| T7869 | 129-130 | DT | denotes | a |
| T7870 | 131-138 | JJ | denotes | 96-well |
| T7871 | 139-144 | NN | denotes | plate |
| T7872 | 145-146 | -LRB- | denotes | ( |
| T7873 | 146-150 | NNP | denotes | Nunc |
| T7874 | 150-151 | -RRB- | denotes | ) |
| T7875 | 152-155 | VBD | denotes | was |
| T7876 | 156-162 | VBN | denotes | coated |
| T7877 | 163-167 | IN | denotes | with |
| T7878 | 168-169 | CD | denotes | 4 |
| T7879 | 170-172 | NN | denotes | μg |
| T7880 | 172-173 | NN | denotes | / |
| T7881 | 173-175 | NN | denotes | ml |
| T7882 | 176-186 | JJ | denotes | monoclonal |
| T7883 | 187-197 | NNS | denotes | antibodies |
| T7884 | 198-205 | IN | denotes | against |
| T7885 | 206-211 | NNP | denotes | IL-17 |
| T7886 | 212-213 | -LRB- | denotes | ( |
| T7887 | 213-214 | NNP | denotes | R |
| T7888 | 215-216 | CC | denotes | & |
| T7889 | 217-218 | NNP | denotes | D |
| T7890 | 219-226 | NNPS | denotes | Systems |
| T7891 | 226-227 | -RRB- | denotes | ) |
| T7892 | 228-230 | IN | denotes | at |
| T7893 | 231-232 | CD | denotes | 4 |
| T7894 | 232-233 | CD | denotes | ° |
| T7895 | 233-234 | NNP | denotes | C |
| T7896 | 235-244 | JJ | denotes | overnight |
| T7897 | 244-245 | . | denotes | . |
| T7898 | 246-251 | IN | denotes | After |
| T7899 | 252-260 | VBG | denotes | blocking |
| T7900 | 261-265 | IN | denotes | with |
| T7901 | 266-284 | JJ | denotes | phosphate-buffered |
| T7902 | 285-293 | CD | denotes | saline/1 |
| T7903 | 293-294 | NN | denotes | % |
| T7904 | 295-301 | JJ | denotes | bovine |
| T7905 | 302-307 | NN | denotes | serum |
| T7906 | 308-315 | NN | denotes | albumin |
| T7907 | 316-317 | -LRB- | denotes | ( |
| T7908 | 317-320 | NNP | denotes | BSA |
| T7909 | 320-321 | -RRB- | denotes | ) |
| T7910 | 321-322 | VBP | denotes | / |
| T7911 | 322-326 | CD | denotes | 0.05 |
| T7912 | 326-327 | NN | denotes | % |
| T7913 | 328-333 | CD | denotes | Tween |
| T7914 | 334-336 | CD | denotes | 20 |
| T7915 | 337-340 | IN | denotes | for |
| T7916 | 341-342 | CD | denotes | 2 |
| T7917 | 343-348 | NNS | denotes | hours |
| T7918 | 349-351 | IN | denotes | at |
| T7919 | 352-356 | NN | denotes | room |
| T7920 | 357-368 | NN | denotes | temperature |
| T7921 | 369-370 | -LRB- | denotes | ( |
| T7922 | 370-372 | CD | denotes | 22 |
| T7923 | 373-375 | CD | denotes | 25 |
| T7924 | 375-376 | NN | denotes | ° |
| T7925 | 376-377 | NNP | denotes | C |
| T7926 | 377-378 | -RRB- | denotes | ) |
| T7927 | 378-379 | , | denotes | , |
| T7928 | 380-384 | NN | denotes | test |
| T7929 | 385-392 | NNS | denotes | samples |
| T7930 | 393-396 | CC | denotes | and |
| T7931 | 397-400 | DT | denotes | the |
| T7932 | 401-409 | JJ | denotes | standard |
| T7933 | 410-421 | JJ | denotes | recombinant |
| T7934 | 422-427 | NNP | denotes | IL-17 |
| T7935 | 428-429 | -LRB- | denotes | ( |
| T7936 | 429-430 | NNP | denotes | R |
| T7937 | 431-432 | CC | denotes | & |
| T7938 | 433-434 | NNP | denotes | D |
| T7939 | 435-442 | NNPS | denotes | Systems |
| T7940 | 442-443 | -RRB- | denotes | ) |
| T7941 | 444-448 | VBD | denotes | were |
| T7942 | 449-454 | VBN | denotes | added |
| T7943 | 455-457 | TO | denotes | to |
| T7944 | 458-461 | DT | denotes | the |
| T7945 | 462-469 | JJ | denotes | 96-well |
| T7946 | 470-475 | NN | denotes | plate |
| T7947 | 476-479 | CC | denotes | and |
| T7948 | 480-489 | VBD | denotes | incubated |
| T7949 | 490-492 | IN | denotes | at |
| T7950 | 493-497 | NN | denotes | room |
| T7951 | 498-509 | NN | denotes | temperature |
| T7952 | 510-513 | IN | denotes | for |
| T7953 | 514-515 | CD | denotes | 2 |
| T7954 | 516-521 | NNS | denotes | hours |
| T7955 | 521-522 | . | denotes | . |
| T7956 | 523-529 | NNS | denotes | Plates |
| T7957 | 530-534 | VBD | denotes | were |
| T7958 | 535-541 | VBN | denotes | washed |
| T7959 | 542-546 | CD | denotes | four |
| T7960 | 547-552 | NNS | denotes | times |
| T7961 | 553-557 | IN | denotes | with |
| T7962 | 558-576 | JJ | denotes | phosphate-buffered |
| T7963 | 577-589 | NNP | denotes | saline/Tween |
| T7964 | 590-592 | CD | denotes | 20 |
| T7965 | 592-593 | , | denotes | , |
| T7966 | 594-597 | CC | denotes | and |
| T7967 | 598-602 | RB | denotes | then |
| T7968 | 603-612 | VBN | denotes | incubated |
| T7969 | 613-617 | IN | denotes | with |
| T7970 | 618-621 | CD | denotes | 500 |
| T7971 | 622-627 | JJ | denotes | ng/ml |
| T7972 | 628-640 | VBN | denotes | biotinylated |
| T7973 | 641-646 | NN | denotes | mouse |
| T7974 | 647-657 | NN | denotes | monoclonal |
| T7975 | 658-668 | NNS | denotes | antibodies |
| T7976 | 669-676 | IN | denotes | against |
| T7977 | 677-682 | NNP | denotes | IL-17 |
| T7978 | 683-684 | -LRB- | denotes | ( |
| T7979 | 684-685 | NNP | denotes | R |
| T7980 | 686-687 | CC | denotes | & |
| T7981 | 688-689 | NNP | denotes | D |
| T7982 | 690-697 | NNPS | denotes | Systems |
| T7983 | 697-698 | -RRB- | denotes | ) |
| T7984 | 699-702 | IN | denotes | for |
| T7985 | 703-704 | CD | denotes | 2 |
| T7986 | 705-710 | NNS | denotes | hours |
| T7987 | 711-713 | IN | denotes | at |
| T7988 | 714-718 | NN | denotes | room |
| T7989 | 719-730 | NN | denotes | temperature |
| T7990 | 730-731 | . | denotes | . |
| T7991 | 732-737 | IN | denotes | After |
| T7992 | 738-745 | VBG | denotes | washing |
| T7993 | 745-746 | , | denotes | , |
| T7994 | 747-759 | NNP | denotes | streptavidin |
| T7995 | 760-768 | JJ | denotes | alkaline |
| T7996 | 769-778 | NN | denotes | phosphate |
| T7997 | 779-790 | JJ | denotes | horseradish |
| T7998 | 791-801 | JJ | denotes | peroxidase |
| T7999 | 802-811 | NN | denotes | conjugate |
| T8000 | 812-813 | -LRB- | denotes | ( |
| T8001 | 813-818 | NNP | denotes | Sigma |
| T8002 | 818-819 | -RRB- | denotes | ) |
| T8003 | 820-823 | VBD | denotes | was |
| T8004 | 824-833 | VBN | denotes | incubated |
| T8005 | 834-837 | IN | denotes | for |
| T8006 | 838-839 | CD | denotes | 2 |
| T8007 | 840-845 | NNS | denotes | hours |
| T8008 | 845-846 | , | denotes | , |
| T8009 | 847-851 | RB | denotes | then |
| T8010 | 852-858 | VBD | denotes | washed |
| T8011 | 859-864 | RB | denotes | again |
| T8012 | 865-868 | CC | denotes | and |
| T8013 | 869-878 | VBN | denotes | incubated |
| T8014 | 879-883 | IN | denotes | with |
| T8015 | 884-885 | CD | denotes | 1 |
| T8016 | 886-891 | NN | denotes | mg/ml |
| T8017 | 892-905 | NN | denotes | p-nitrophenyl |
| T8018 | 906-915 | NN | denotes | phosphate |
| T8019 | 916-917 | -LRB- | denotes | ( |
| T8020 | 917-922 | NNP | denotes | Sigma |
| T8021 | 922-923 | -RRB- | denotes | ) |
| T8022 | 924-933 | VBN | denotes | dissolved |
| T8023 | 934-936 | IN | denotes | in |
| T8024 | 937-951 | NN | denotes | diethanolamine |
| T8025 | 952-953 | -LRB- | denotes | ( |
| T8026 | 953-958 | NNP | denotes | Sigma |
| T8027 | 958-959 | -RRB- | denotes | ) |
| T8028 | 960-962 | TO | denotes | to |
| T8029 | 963-970 | VB | denotes | develop |
| T8030 | 971-974 | DT | denotes | the |
| T8031 | 975-980 | NN | denotes | color |
| T8032 | 981-989 | NN | denotes | reaction |
| T8033 | 989-990 | . | denotes | . |
| T8034 | 991-994 | DT | denotes | The |
| T8035 | 995-1003 | NN | denotes | reaction |
| T8036 | 1004-1007 | VBD | denotes | was |
| T8037 | 1008-1015 | VBN | denotes | stopped |
| T8038 | 1016-1018 | IN | denotes | by |
| T8039 | 1019-1022 | DT | denotes | the |
| T8040 | 1023-1031 | NN | denotes | addition |
| T8041 | 1032-1034 | IN | denotes | of |
| T8042 | 1035-1036 | CD | denotes | 1 |
| T8043 | 1037-1038 | NNP | denotes | M |
| T8044 | 1039-1043 | NNP | denotes | NaOH |
| T8045 | 1044-1047 | CC | denotes | and |
| T8046 | 1048-1051 | DT | denotes | the |
| T8047 | 1052-1059 | JJ | denotes | optical |
| T8048 | 1060-1067 | NN | denotes | density |
| T8049 | 1068-1070 | IN | denotes | of |
| T8050 | 1071-1075 | DT | denotes | each |
| T8051 | 1076-1080 | NN | denotes | well |
| T8052 | 1081-1084 | VBD | denotes | was |
| T8053 | 1085-1089 | VBN | denotes | read |
| T8054 | 1090-1092 | IN | denotes | at |
| T8055 | 1093-1096 | CD | denotes | 405 |
| T8056 | 1097-1099 | NN | denotes | nm |
| T8057 | 1099-1100 | . | denotes | . |
| T8058 | 1101-1104 | DT | denotes | The |
| T8059 | 1105-1110 | JJR | denotes | lower |
| T8060 | 1111-1116 | NN | denotes | limit |
| T8061 | 1117-1119 | IN | denotes | of |
| T8062 | 1120-1125 | NNP | denotes | IL-17 |
| T8063 | 1126-1135 | NN | denotes | detection |
| T8064 | 1136-1139 | VBD | denotes | was |
| T8065 | 1140-1142 | CD | denotes | 10 |
| T8066 | 1143-1148 | NN | denotes | pg/ml |
| T8067 | 1148-1149 | . | denotes | . |
| T8068 | 1150-1161 | JJ | denotes | Recombinant |
| T8069 | 1162-1167 | JJ | denotes | human |
| T8070 | 1168-1173 | NN | denotes | IL-17 |
| T8071 | 1174-1181 | VBN | denotes | diluted |
| T8072 | 1182-1184 | IN | denotes | in |
| T8073 | 1185-1192 | NN | denotes | culture |
| T8074 | 1193-1199 | NN | denotes | medium |
| T8075 | 1200-1203 | VBD | denotes | was |
| T8076 | 1204-1208 | VBN | denotes | used |
| T8077 | 1209-1211 | IN | denotes | as |
| T8078 | 1212-1213 | DT | denotes | a |
| T8079 | 1214-1225 | NN | denotes | calibration |
| T8080 | 1226-1234 | NN | denotes | standard |
| T8081 | 1234-1235 | , | denotes | , |
| T8082 | 1236-1243 | VBG | denotes | ranging |
| T8083 | 1244-1248 | IN | denotes | from |
| T8084 | 1249-1251 | CD | denotes | 10 |
| T8085 | 1252-1254 | TO | denotes | to |
| T8086 | 1255-1259 | CD | denotes | 2000 |
| T8087 | 1260-1265 | NN | denotes | pg/ml |
| T8088 | 1265-1266 | . | denotes | . |
| T8089 | 1267-1268 | DT | denotes | A |
| T8090 | 1269-1277 | JJ | denotes | standard |
| T8091 | 1278-1283 | NN | denotes | curve |
| T8092 | 1284-1287 | VBD | denotes | was |
| T8093 | 1288-1293 | VBN | denotes | drawn |
| T8094 | 1294-1296 | IN | denotes | by |
| T8095 | 1297-1305 | VBG | denotes | plotting |
| T8096 | 1306-1313 | JJ | denotes | optical |
| T8097 | 1314-1321 | NN | denotes | density |
| T8098 | 1322-1329 | IN | denotes | against |
| T8099 | 1330-1333 | DT | denotes | the |
| T8100 | 1334-1337 | VB | denotes | log |
| T8101 | 1338-1340 | IN | denotes | of |
| T8102 | 1341-1344 | DT | denotes | the |
| T8103 | 1345-1358 | NN | denotes | concentration |
| T8104 | 1359-1361 | IN | denotes | of |
| T8105 | 1362-1373 | JJ | denotes | recombinant |
| T8106 | 1374-1383 | NNS | denotes | cytokines |
| T8107 | 1383-1384 | , | denotes | , |
| T8108 | 1385-1388 | CC | denotes | and |
| T8109 | 1389-1393 | VBN | denotes | used |
| T8110 | 1394-1397 | IN | denotes | for |
| T8111 | 1398-1411 | NN | denotes | determination |
| T8112 | 1412-1414 | IN | denotes | of |
| T8113 | 1415-1420 | NNP | denotes | IL-17 |
| T8114 | 1421-1423 | IN | denotes | in |
| T8115 | 1424-1428 | NN | denotes | test |
| T8116 | 1429-1436 | NNS | denotes | samples |
| T8117 | 1436-1437 | . | denotes | . |
| R4201 | T7850 | T7851 | compound | culture,supernatants |
| R4202 | T7851 | T7849 | pobj | supernatants,in |
| R4203 | T7852 | T7853 | auxpass | was,measured |
| R4204 | T7853 | T7853 | ROOT | measured,measured |
| R4205 | T7854 | T7853 | agent | by,measured |
| R4206 | T7855 | T7858 | nmod | sandwich,assay |
| R4207 | T7856 | T7858 | amod | enzyme-linked,assay |
| R4208 | T7857 | T7858 | compound | immunosorbent,assay |
| R4209 | T7858 | T7854 | pobj | assay,by |
| R4210 | T7859 | T7860 | mark | as,described |
| R4211 | T7860 | T7853 | advcl | described,measured |
| R4212 | T7861 | T7864 | advmod | previously,] |
| R4213 | T7862 | T7864 | nmod | [,] |
| R4214 | T7863 | T7864 | nummod | 20,] |
| R4215 | T7864 | T7860 | dobj | ],described |
| R4216 | T7865 | T7853 | punct | .,measured |
| R4217 | T7866 | T7876 | prep | In,coated |
| R4218 | T7867 | T7866 | pobj | brief,In |
| R4219 | T7868 | T7876 | punct | ",",coated |
| R4220 | T7869 | T7871 | det | a,plate |
| R4221 | T7870 | T7871 | amod | 96-well,plate |
| R4222 | T7871 | T7876 | nsubjpass | plate,coated |
| R4223 | T7872 | T7873 | punct | (,Nunc |
| R4224 | T7873 | T7871 | appos | Nunc,plate |
| R4225 | T7874 | T7873 | punct | ),Nunc |
| R4226 | T7875 | T7876 | auxpass | was,coated |
| R4227 | T7876 | T7876 | ROOT | coated,coated |
| R4228 | T7877 | T7876 | prep | with,coated |
| R4229 | T7878 | T7879 | nummod | 4,μg |
| R4230 | T7879 | T7883 | amod | μg,antibodies |
| R4231 | T7880 | T7883 | nmod | /,antibodies |
| R4232 | T7881 | T7883 | amod | ml,antibodies |
| R4233 | T7882 | T7883 | amod | monoclonal,antibodies |
| R4234 | T7883 | T7877 | pobj | antibodies,with |
| R4235 | T7884 | T7883 | prep | against,antibodies |
| R4236 | T7885 | T7884 | pobj | IL-17,against |
| R4237 | T7886 | T7885 | punct | (,IL-17 |
| R4238 | T7887 | T7885 | conj | R,IL-17 |
| R4239 | T7888 | T7887 | cc | &,R |
| R4240 | T7889 | T7890 | compound | D,Systems |
| R4241 | T7890 | T7887 | conj | Systems,R |
| R4242 | T7891 | T7885 | punct | ),IL-17 |
| R4243 | T7892 | T7876 | prep | at,coated |
| R4244 | T7893 | T7894 | nummod | 4,° |
| R4245 | T7894 | T7895 | nummod | °,C |
| R4246 | T7895 | T7896 | compound | C,overnight |
| R4247 | T7896 | T7892 | pobj | overnight,at |
| R4248 | T7897 | T7876 | punct | .,coated |
| R4249 | T7898 | T7910 | prep | After,/ |
| R4250 | T7899 | T7898 | pcomp | blocking,After |
| R4251 | T7900 | T7899 | prep | with,blocking |
| R4252 | T7901 | T7906 | amod | phosphate-buffered,albumin |
| R4253 | T7902 | T7903 | nummod | saline/1,% |
| R4254 | T7903 | T7906 | compound | %,albumin |
| R4255 | T7904 | T7905 | amod | bovine,serum |
| R4256 | T7905 | T7906 | compound | serum,albumin |
| R4257 | T7906 | T7900 | pobj | albumin,with |
| R4258 | T7907 | T7908 | punct | (,BSA |
| R4259 | T7908 | T7906 | npadvmod | BSA,albumin |
| R4260 | T7909 | T7910 | punct | ),/ |
| R4261 | T7910 | T7910 | ROOT | /,/ |
| R4262 | T7911 | T7912 | nummod | 0.05,% |
| R4263 | T7912 | T7929 | nmod | %,samples |
| R4264 | T7913 | T7914 | nummod | Tween,20 |
| R4265 | T7914 | T7929 | nummod | 20,samples |
| R4266 | T7915 | T7914 | prep | for,20 |
| R4267 | T7916 | T7917 | nummod | 2,hours |
| R4268 | T7917 | T7915 | pobj | hours,for |
| R4269 | T7918 | T7917 | prep | at,hours |
| R4270 | T7919 | T7920 | compound | room,temperature |
| R4271 | T7920 | T7918 | pobj | temperature,at |
| R4272 | T7921 | T7917 | punct | (,hours |
| R4273 | T7922 | T7917 | nummod | 22,hours |
| R4274 | T7923 | T7925 | nummod | 25,C |
| R4275 | T7924 | T7925 | compound | °,C |
| R4276 | T7925 | T7917 | appos | C,hours |
| R4277 | T7926 | T7914 | punct | ),20 |
| R4278 | T7927 | T7914 | punct | ",",20 |
| R4279 | T7928 | T7929 | compound | test,samples |
| R4280 | T7929 | T7910 | dobj | samples,/ |
| R4281 | T7930 | T7929 | cc | and,samples |
| R4282 | T7931 | T7934 | det | the,IL-17 |
| R4283 | T7932 | T7934 | amod | standard,IL-17 |
| R4284 | T7933 | T7934 | compound | recombinant,IL-17 |
| R4285 | T7934 | T7929 | conj | IL-17,samples |
| R4286 | T7935 | T7942 | punct | (,added |
| R4287 | T7936 | T7942 | nsubjpass | R,added |
| R4288 | T7937 | T7936 | cc | &,R |
| R4289 | T7938 | T7939 | compound | D,Systems |
| R4290 | T7939 | T7936 | conj | Systems,R |
| R4291 | T7940 | T7942 | punct | ),added |
| R4292 | T7941 | T7942 | auxpass | were,added |
| R4293 | T7942 | T7934 | parataxis | added,IL-17 |
| R4294 | T7943 | T7942 | prep | to,added |
| R4295 | T7944 | T7946 | det | the,plate |
| R4296 | T7945 | T7946 | amod | 96-well,plate |
| R4297 | T7946 | T7943 | pobj | plate,to |
| R4298 | T7947 | T7942 | cc | and,added |
| R4299 | T7948 | T7942 | conj | incubated,added |
| R4300 | T7949 | T7948 | prep | at,incubated |
| R4301 | T7950 | T7951 | compound | room,temperature |
| R4302 | T7951 | T7949 | pobj | temperature,at |
| R4303 | T7952 | T7948 | prep | for,incubated |
| R4304 | T7953 | T7954 | nummod | 2,hours |
| R4305 | T7954 | T7952 | pobj | hours,for |
| R4306 | T7955 | T7942 | punct | .,added |
| R4307 | T7956 | T7958 | nsubjpass | Plates,washed |
| R4308 | T7957 | T7958 | auxpass | were,washed |
| R4309 | T7958 | T7958 | ROOT | washed,washed |
| R4310 | T7959 | T7960 | nummod | four,times |
| R4311 | T7960 | T7958 | npadvmod | times,washed |
| R4312 | T7961 | T7958 | prep | with,washed |
| R4313 | T7962 | T7963 | compound | phosphate-buffered,saline/Tween |
| R4314 | T7963 | T7961 | pobj | saline/Tween,with |
| R4315 | T7964 | T7963 | nummod | 20,saline/Tween |
| R4316 | T7965 | T7958 | punct | ",",washed |
| R4317 | T7966 | T7958 | cc | and,washed |
| R4318 | T7967 | T7968 | advmod | then,incubated |
| R4319 | T7968 | T7958 | conj | incubated,washed |
| R4320 | T7969 | T7968 | prep | with,incubated |
| R4321 | T7970 | T7975 | nummod | 500,antibodies |
| R4322 | T7971 | T7975 | amod | ng/ml,antibodies |
| R4323 | T7972 | T7975 | amod | biotinylated,antibodies |
| R4324 | T7973 | T7975 | compound | mouse,antibodies |
| R4325 | T7974 | T7975 | compound | monoclonal,antibodies |
| R4326 | T7975 | T7969 | pobj | antibodies,with |
| R4327 | T7976 | T7975 | prep | against,antibodies |
| R4328 | T7977 | T7976 | pobj | IL-17,against |
| R4329 | T7978 | T7977 | punct | (,IL-17 |
| R4330 | T7979 | T7977 | conj | R,IL-17 |
| R4331 | T7980 | T7979 | cc | &,R |
| R4332 | T7981 | T7982 | compound | D,Systems |
| R4333 | T7982 | T7979 | conj | Systems,R |
| R4334 | T7983 | T7968 | punct | ),incubated |
| R4335 | T7984 | T7968 | prep | for,incubated |
| R4336 | T7985 | T7986 | nummod | 2,hours |
| R4337 | T7986 | T7984 | pobj | hours,for |
| R4338 | T7987 | T7986 | prep | at,hours |
| R4339 | T7988 | T7989 | compound | room,temperature |
| R4340 | T7989 | T7987 | pobj | temperature,at |
| R4341 | T7990 | T7958 | punct | .,washed |
| R4342 | T7991 | T8004 | prep | After,incubated |
| R4343 | T7992 | T7991 | pcomp | washing,After |
| R4344 | T7993 | T8004 | punct | ",",incubated |
| R4345 | T7994 | T8004 | nsubjpass | streptavidin,incubated |
| R4346 | T7995 | T7996 | amod | alkaline,phosphate |
| R4347 | T7996 | T8003 | nsubj | phosphate,was |
| R4348 | T7997 | T7999 | amod | horseradish,conjugate |
| R4349 | T7998 | T7999 | amod | peroxidase,conjugate |
| R4350 | T7999 | T7996 | appos | conjugate,phosphate |
| R4351 | T8000 | T8001 | punct | (,Sigma |
| R4352 | T8001 | T7999 | appos | Sigma,conjugate |
| R4353 | T8002 | T7999 | punct | ),conjugate |
| R4354 | T8003 | T8004 | auxpass | was,incubated |
| R4355 | T8004 | T8004 | ROOT | incubated,incubated |
| R4356 | T8005 | T8004 | prep | for,incubated |
| R4357 | T8006 | T8007 | nummod | 2,hours |
| R4358 | T8007 | T8005 | pobj | hours,for |
| R4359 | T8008 | T8010 | punct | ",",washed |
| R4360 | T8009 | T8010 | advmod | then,washed |
| R4361 | T8010 | T8004 | dep | washed,incubated |
| R4362 | T8011 | T8010 | advmod | again,washed |
| R4363 | T8012 | T8010 | cc | and,washed |
| R4364 | T8013 | T8010 | conj | incubated,washed |
| R4365 | T8014 | T8013 | prep | with,incubated |
| R4366 | T8015 | T8016 | nummod | 1,mg/ml |
| R4367 | T8016 | T8018 | nmod | mg/ml,phosphate |
| R4368 | T8017 | T8018 | compound | p-nitrophenyl,phosphate |
| R4369 | T8018 | T8014 | pobj | phosphate,with |
| R4370 | T8019 | T8020 | punct | (,Sigma |
| R4371 | T8020 | T8018 | appos | Sigma,phosphate |
| R4372 | T8021 | T8013 | punct | ),incubated |
| R4373 | T8022 | T8010 | conj | dissolved,washed |
| R4374 | T8023 | T8022 | prep | in,dissolved |
| R4375 | T8024 | T8023 | pobj | diethanolamine,in |
| R4376 | T8025 | T8026 | punct | (,Sigma |
| R4377 | T8026 | T8024 | appos | Sigma,diethanolamine |
| R4378 | T8027 | T8024 | punct | ),diethanolamine |
| R4379 | T8028 | T8029 | aux | to,develop |
| R4380 | T8029 | T8022 | advcl | develop,dissolved |
| R4381 | T8030 | T8032 | det | the,reaction |
| R4382 | T8031 | T8032 | compound | color,reaction |
| R4383 | T8032 | T8029 | dobj | reaction,develop |
| R4384 | T8033 | T8004 | punct | .,incubated |
| R4385 | T8034 | T8035 | det | The,reaction |
| R4386 | T8035 | T8037 | nsubjpass | reaction,stopped |
| R4387 | T8036 | T8037 | auxpass | was,stopped |
| R4388 | T8037 | T8037 | ROOT | stopped,stopped |
| R4389 | T8038 | T8037 | agent | by,stopped |
| R4390 | T8039 | T8040 | det | the,addition |
| R4391 | T8040 | T8038 | pobj | addition,by |
| R4392 | T8041 | T8040 | prep | of,addition |
| R4393 | T8042 | T8044 | nummod | 1,NaOH |
| R4394 | T8043 | T8044 | compound | M,NaOH |
| R4395 | T8044 | T8041 | pobj | NaOH,of |
| R4396 | T8045 | T8044 | cc | and,NaOH |
| R4397 | T8046 | T8048 | det | the,density |
| R4398 | T8047 | T8048 | amod | optical,density |
| R4399 | T8048 | T8044 | conj | density,NaOH |
| R4400 | T8049 | T8048 | prep | of,density |
| R4401 | T8050 | T8051 | det | each,well |
| R4402 | T8051 | T8049 | pobj | well,of |
| R4403 | T8052 | T8053 | auxpass | was,read |
| R4404 | T8053 | T8037 | conj | read,stopped |
| R4405 | T8054 | T8053 | prep | at,read |
| R4406 | T8055 | T8056 | nummod | 405,nm |
| R4407 | T8056 | T8054 | pobj | nm,at |
| R4408 | T8057 | T8053 | punct | .,read |
| R4409 | T8058 | T8060 | det | The,limit |
| R4410 | T8059 | T8060 | amod | lower,limit |
| R4411 | T8060 | T8064 | nsubj | limit,was |
| R4412 | T8061 | T8060 | prep | of,limit |
| R4413 | T8062 | T8063 | compound | IL-17,detection |
| R4414 | T8063 | T8061 | pobj | detection,of |
| R4415 | T8064 | T8064 | ROOT | was,was |
| R4416 | T8065 | T8066 | nummod | 10,pg/ml |
| R4417 | T8066 | T8064 | attr | pg/ml,was |
| R4418 | T8067 | T8064 | punct | .,was |
| R4419 | T8068 | T8070 | amod | Recombinant,IL-17 |
| R4420 | T8069 | T8070 | amod | human,IL-17 |
| R4421 | T8070 | T8071 | nsubj | IL-17,diluted |
| R4422 | T8071 | T8076 | nsubjpass | diluted,used |
| R4423 | T8072 | T8071 | prep | in,diluted |
| R4424 | T8073 | T8074 | compound | culture,medium |
| R4425 | T8074 | T8072 | pobj | medium,in |
| R4426 | T8075 | T8076 | auxpass | was,used |
| R4427 | T8076 | T8076 | ROOT | used,used |
| R4428 | T8077 | T8076 | prep | as,used |
| R4429 | T8078 | T8080 | det | a,standard |
| R4430 | T8079 | T8080 | compound | calibration,standard |
| R4431 | T8080 | T8077 | pobj | standard,as |
| R4432 | T8081 | T8076 | punct | ",",used |
| R4433 | T8082 | T8076 | advcl | ranging,used |
| R4434 | T8083 | T8082 | prep | from,ranging |
| R4435 | T8084 | T8083 | pobj | 10,from |
| R4436 | T8085 | T8083 | prep | to,from |
| R4437 | T8086 | T8087 | det | 2000,pg/ml |
| R4438 | T8087 | T8085 | pobj | pg/ml,to |
| R4439 | T8088 | T8076 | punct | .,used |
| R4440 | T8089 | T8091 | det | A,curve |
| R4441 | T8090 | T8091 | amod | standard,curve |
| R4442 | T8091 | T8093 | nsubjpass | curve,drawn |
| R4443 | T8092 | T8093 | auxpass | was,drawn |
| R4444 | T8093 | T8093 | ROOT | drawn,drawn |
| R4445 | T8094 | T8093 | agent | by,drawn |
| R4446 | T8095 | T8094 | pcomp | plotting,by |
| R4447 | T8096 | T8097 | amod | optical,density |
| R4448 | T8097 | T8095 | dobj | density,plotting |
| R4449 | T8098 | T8097 | prep | against,density |
| R4450 | T8099 | T8100 | det | the,log |
| R4451 | T8100 | T8098 | pobj | log,against |
| R4452 | T8101 | T8100 | prep | of,log |
| R4453 | T8102 | T8103 | det | the,concentration |
| R4454 | T8103 | T8101 | pobj | concentration,of |
| R4455 | T8104 | T8103 | prep | of,concentration |
| R4456 | T8105 | T8106 | amod | recombinant,cytokines |
| R4457 | T8106 | T8104 | pobj | cytokines,of |
| R4458 | T8107 | T8093 | punct | ",",drawn |
| R4459 | T8108 | T8093 | cc | and,drawn |
| R4460 | T8109 | T8093 | conj | used,drawn |
| R4461 | T8110 | T8109 | prep | for,used |
| R4462 | T8111 | T8110 | pobj | determination,for |
| R4463 | T8112 | T8111 | prep | of,determination |
| R4464 | T8113 | T8112 | pobj | IL-17,of |
| R4465 | T8114 | T8111 | prep | in,determination |
| R4466 | T8115 | T8116 | compound | test,samples |
| R4467 | T8116 | T8114 | pobj | samples,in |
| R4468 | T8117 | T8093 | punct | .,drawn |