PMC:1064895 / 9420-10900 24 Projects
Annnotations
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7843 | 0-13 | JJ | denotes | Enzyme-linked |
T7844 | 14-27 | JJ | denotes | immunosorbent |
T7845 | 28-33 | NN | denotes | assay |
T7846 | 34-36 | IN | denotes | of |
T7847 | 37-42 | NNP | denotes | IL-17 |
T7848 | 43-48 | NNP | denotes | IL-17 |
T7849 | 49-51 | IN | denotes | in |
T7850 | 52-59 | NN | denotes | culture |
T7851 | 60-72 | NNS | denotes | supernatants |
T7852 | 73-76 | VBD | denotes | was |
T7853 | 77-85 | VBN | denotes | measured |
T7854 | 86-88 | IN | denotes | by |
T7855 | 89-97 | NN | denotes | sandwich |
T7856 | 98-111 | JJ | denotes | enzyme-linked |
T7857 | 112-125 | JJ | denotes | immunosorbent |
T7858 | 126-131 | NN | denotes | assay |
T7859 | 132-134 | IN | denotes | as |
T7860 | 135-144 | VBN | denotes | described |
T7861 | 145-155 | RB | denotes | previously |
T7862 | 156-157 | NNP | denotes | [ |
T7863 | 157-159 | CD | denotes | 20 |
T7864 | 159-160 | NNP | denotes | ] |
T7865 | 160-161 | . | denotes | . |
T7866 | 162-164 | IN | denotes | In |
T7867 | 165-170 | NN | denotes | brief |
T7868 | 170-171 | , | denotes | , |
T7869 | 172-173 | DT | denotes | a |
T7870 | 174-181 | JJ | denotes | 96-well |
T7871 | 182-187 | NN | denotes | plate |
T7872 | 188-189 | -LRB- | denotes | ( |
T7873 | 189-193 | NNP | denotes | Nunc |
T7874 | 193-194 | -RRB- | denotes | ) |
T7875 | 195-198 | VBD | denotes | was |
T7876 | 199-205 | VBN | denotes | coated |
T7877 | 206-210 | IN | denotes | with |
T7878 | 211-212 | CD | denotes | 4 |
T7879 | 213-215 | NN | denotes | μg |
T7880 | 215-216 | NN | denotes | / |
T7881 | 216-218 | NN | denotes | ml |
T7882 | 219-229 | JJ | denotes | monoclonal |
T7883 | 230-240 | NNS | denotes | antibodies |
T7884 | 241-248 | IN | denotes | against |
T7885 | 249-254 | NNP | denotes | IL-17 |
T7886 | 255-256 | -LRB- | denotes | ( |
T7887 | 256-257 | NNP | denotes | R |
T7888 | 258-259 | CC | denotes | & |
T7889 | 260-261 | NNP | denotes | D |
T7890 | 262-269 | NNPS | denotes | Systems |
T7891 | 269-270 | -RRB- | denotes | ) |
T7892 | 271-273 | IN | denotes | at |
T7893 | 274-275 | CD | denotes | 4 |
T7894 | 275-276 | CD | denotes | ° |
T7895 | 276-277 | NNP | denotes | C |
T7896 | 278-287 | JJ | denotes | overnight |
T7897 | 287-288 | . | denotes | . |
T7898 | 289-294 | IN | denotes | After |
T7899 | 295-303 | VBG | denotes | blocking |
T7900 | 304-308 | IN | denotes | with |
T7901 | 309-327 | JJ | denotes | phosphate-buffered |
T7902 | 328-336 | CD | denotes | saline/1 |
T7903 | 336-337 | NN | denotes | % |
T7904 | 338-344 | JJ | denotes | bovine |
T7905 | 345-350 | NN | denotes | serum |
T7906 | 351-358 | NN | denotes | albumin |
T7907 | 359-360 | -LRB- | denotes | ( |
T7908 | 360-363 | NNP | denotes | BSA |
T7909 | 363-364 | -RRB- | denotes | ) |
T7910 | 364-365 | VBP | denotes | / |
T7911 | 365-369 | CD | denotes | 0.05 |
T7912 | 369-370 | NN | denotes | % |
T7913 | 371-376 | CD | denotes | Tween |
T7914 | 377-379 | CD | denotes | 20 |
T7915 | 380-383 | IN | denotes | for |
T7916 | 384-385 | CD | denotes | 2 |
T7917 | 386-391 | NNS | denotes | hours |
T7918 | 392-394 | IN | denotes | at |
T7919 | 395-399 | NN | denotes | room |
T7920 | 400-411 | NN | denotes | temperature |
T7921 | 412-413 | -LRB- | denotes | ( |
T7922 | 413-415 | CD | denotes | 22 |
T7923 | 416-418 | CD | denotes | 25 |
T7924 | 418-419 | NN | denotes | ° |
T7925 | 419-420 | NNP | denotes | C |
T7926 | 420-421 | -RRB- | denotes | ) |
T7927 | 421-422 | , | denotes | , |
T7928 | 423-427 | NN | denotes | test |
T7929 | 428-435 | NNS | denotes | samples |
T7930 | 436-439 | CC | denotes | and |
T7931 | 440-443 | DT | denotes | the |
T7932 | 444-452 | JJ | denotes | standard |
T7933 | 453-464 | JJ | denotes | recombinant |
T7934 | 465-470 | NNP | denotes | IL-17 |
T7935 | 471-472 | -LRB- | denotes | ( |
T7936 | 472-473 | NNP | denotes | R |
T7937 | 474-475 | CC | denotes | & |
T7938 | 476-477 | NNP | denotes | D |
T7939 | 478-485 | NNPS | denotes | Systems |
T7940 | 485-486 | -RRB- | denotes | ) |
T7941 | 487-491 | VBD | denotes | were |
T7942 | 492-497 | VBN | denotes | added |
T7943 | 498-500 | TO | denotes | to |
T7944 | 501-504 | DT | denotes | the |
T7945 | 505-512 | JJ | denotes | 96-well |
T7946 | 513-518 | NN | denotes | plate |
T7947 | 519-522 | CC | denotes | and |
T7948 | 523-532 | VBD | denotes | incubated |
T7949 | 533-535 | IN | denotes | at |
T7950 | 536-540 | NN | denotes | room |
T7951 | 541-552 | NN | denotes | temperature |
T7952 | 553-556 | IN | denotes | for |
T7953 | 557-558 | CD | denotes | 2 |
T7954 | 559-564 | NNS | denotes | hours |
T7955 | 564-565 | . | denotes | . |
T7956 | 566-572 | NNS | denotes | Plates |
T7957 | 573-577 | VBD | denotes | were |
T7958 | 578-584 | VBN | denotes | washed |
T7959 | 585-589 | CD | denotes | four |
T7960 | 590-595 | NNS | denotes | times |
T7961 | 596-600 | IN | denotes | with |
T7962 | 601-619 | JJ | denotes | phosphate-buffered |
T7963 | 620-632 | NNP | denotes | saline/Tween |
T7964 | 633-635 | CD | denotes | 20 |
T7965 | 635-636 | , | denotes | , |
T7966 | 637-640 | CC | denotes | and |
T7967 | 641-645 | RB | denotes | then |
T7968 | 646-655 | VBN | denotes | incubated |
T7969 | 656-660 | IN | denotes | with |
T7970 | 661-664 | CD | denotes | 500 |
T7971 | 665-670 | JJ | denotes | ng/ml |
T7972 | 671-683 | VBN | denotes | biotinylated |
T7973 | 684-689 | NN | denotes | mouse |
T7974 | 690-700 | NN | denotes | monoclonal |
T7975 | 701-711 | NNS | denotes | antibodies |
T7976 | 712-719 | IN | denotes | against |
T7977 | 720-725 | NNP | denotes | IL-17 |
T7978 | 726-727 | -LRB- | denotes | ( |
T7979 | 727-728 | NNP | denotes | R |
T7980 | 729-730 | CC | denotes | & |
T7981 | 731-732 | NNP | denotes | D |
T7982 | 733-740 | NNPS | denotes | Systems |
T7983 | 740-741 | -RRB- | denotes | ) |
T7984 | 742-745 | IN | denotes | for |
T7985 | 746-747 | CD | denotes | 2 |
T7986 | 748-753 | NNS | denotes | hours |
T7987 | 754-756 | IN | denotes | at |
T7988 | 757-761 | NN | denotes | room |
T7989 | 762-773 | NN | denotes | temperature |
T7990 | 773-774 | . | denotes | . |
T7991 | 775-780 | IN | denotes | After |
T7992 | 781-788 | VBG | denotes | washing |
T7993 | 788-789 | , | denotes | , |
T7994 | 790-802 | NNP | denotes | streptavidin |
T7995 | 803-811 | JJ | denotes | alkaline |
T7996 | 812-821 | NN | denotes | phosphate |
T7997 | 822-833 | JJ | denotes | horseradish |
T7998 | 834-844 | JJ | denotes | peroxidase |
T7999 | 845-854 | NN | denotes | conjugate |
T8000 | 855-856 | -LRB- | denotes | ( |
T8001 | 856-861 | NNP | denotes | Sigma |
T8002 | 861-862 | -RRB- | denotes | ) |
T8003 | 863-866 | VBD | denotes | was |
T8004 | 867-876 | VBN | denotes | incubated |
T8005 | 877-880 | IN | denotes | for |
T8006 | 881-882 | CD | denotes | 2 |
T8007 | 883-888 | NNS | denotes | hours |
T8008 | 888-889 | , | denotes | , |
T8009 | 890-894 | RB | denotes | then |
T8010 | 895-901 | VBD | denotes | washed |
T8011 | 902-907 | RB | denotes | again |
T8012 | 908-911 | CC | denotes | and |
T8013 | 912-921 | VBN | denotes | incubated |
T8014 | 922-926 | IN | denotes | with |
T8015 | 927-928 | CD | denotes | 1 |
T8016 | 929-934 | NN | denotes | mg/ml |
T8017 | 935-948 | NN | denotes | p-nitrophenyl |
T8018 | 949-958 | NN | denotes | phosphate |
T8019 | 959-960 | -LRB- | denotes | ( |
T8020 | 960-965 | NNP | denotes | Sigma |
T8021 | 965-966 | -RRB- | denotes | ) |
T8022 | 967-976 | VBN | denotes | dissolved |
T8023 | 977-979 | IN | denotes | in |
T8024 | 980-994 | NN | denotes | diethanolamine |
T8025 | 995-996 | -LRB- | denotes | ( |
T8026 | 996-1001 | NNP | denotes | Sigma |
T8027 | 1001-1002 | -RRB- | denotes | ) |
T8028 | 1003-1005 | TO | denotes | to |
T8029 | 1006-1013 | VB | denotes | develop |
T8030 | 1014-1017 | DT | denotes | the |
T8031 | 1018-1023 | NN | denotes | color |
T8032 | 1024-1032 | NN | denotes | reaction |
T8033 | 1032-1033 | . | denotes | . |
T8034 | 1034-1037 | DT | denotes | The |
T8035 | 1038-1046 | NN | denotes | reaction |
T8036 | 1047-1050 | VBD | denotes | was |
T8037 | 1051-1058 | VBN | denotes | stopped |
T8038 | 1059-1061 | IN | denotes | by |
T8039 | 1062-1065 | DT | denotes | the |
T8040 | 1066-1074 | NN | denotes | addition |
T8041 | 1075-1077 | IN | denotes | of |
T8042 | 1078-1079 | CD | denotes | 1 |
T8043 | 1080-1081 | NNP | denotes | M |
T8044 | 1082-1086 | NNP | denotes | NaOH |
T8045 | 1087-1090 | CC | denotes | and |
T8046 | 1091-1094 | DT | denotes | the |
T8047 | 1095-1102 | JJ | denotes | optical |
T8048 | 1103-1110 | NN | denotes | density |
T8049 | 1111-1113 | IN | denotes | of |
T8050 | 1114-1118 | DT | denotes | each |
T8051 | 1119-1123 | NN | denotes | well |
T8052 | 1124-1127 | VBD | denotes | was |
T8053 | 1128-1132 | VBN | denotes | read |
T8054 | 1133-1135 | IN | denotes | at |
T8055 | 1136-1139 | CD | denotes | 405 |
T8056 | 1140-1142 | NN | denotes | nm |
T8057 | 1142-1143 | . | denotes | . |
T8058 | 1144-1147 | DT | denotes | The |
T8059 | 1148-1153 | JJR | denotes | lower |
T8060 | 1154-1159 | NN | denotes | limit |
T8061 | 1160-1162 | IN | denotes | of |
T8062 | 1163-1168 | NNP | denotes | IL-17 |
T8063 | 1169-1178 | NN | denotes | detection |
T8064 | 1179-1182 | VBD | denotes | was |
T8065 | 1183-1185 | CD | denotes | 10 |
T8066 | 1186-1191 | NN | denotes | pg/ml |
T8067 | 1191-1192 | . | denotes | . |
T8068 | 1193-1204 | JJ | denotes | Recombinant |
T8069 | 1205-1210 | JJ | denotes | human |
T8070 | 1211-1216 | NN | denotes | IL-17 |
T8071 | 1217-1224 | VBN | denotes | diluted |
T8072 | 1225-1227 | IN | denotes | in |
T8073 | 1228-1235 | NN | denotes | culture |
T8074 | 1236-1242 | NN | denotes | medium |
T8075 | 1243-1246 | VBD | denotes | was |
T8076 | 1247-1251 | VBN | denotes | used |
T8077 | 1252-1254 | IN | denotes | as |
T8078 | 1255-1256 | DT | denotes | a |
T8079 | 1257-1268 | NN | denotes | calibration |
T8080 | 1269-1277 | NN | denotes | standard |
T8081 | 1277-1278 | , | denotes | , |
T8082 | 1279-1286 | VBG | denotes | ranging |
T8083 | 1287-1291 | IN | denotes | from |
T8084 | 1292-1294 | CD | denotes | 10 |
T8085 | 1295-1297 | TO | denotes | to |
T8086 | 1298-1302 | CD | denotes | 2000 |
T8087 | 1303-1308 | NN | denotes | pg/ml |
T8088 | 1308-1309 | . | denotes | . |
T8089 | 1310-1311 | DT | denotes | A |
T8090 | 1312-1320 | JJ | denotes | standard |
T8091 | 1321-1326 | NN | denotes | curve |
T8092 | 1327-1330 | VBD | denotes | was |
T8093 | 1331-1336 | VBN | denotes | drawn |
T8094 | 1337-1339 | IN | denotes | by |
T8095 | 1340-1348 | VBG | denotes | plotting |
T8096 | 1349-1356 | JJ | denotes | optical |
T8097 | 1357-1364 | NN | denotes | density |
T8098 | 1365-1372 | IN | denotes | against |
T8099 | 1373-1376 | DT | denotes | the |
T8100 | 1377-1380 | VB | denotes | log |
T8101 | 1381-1383 | IN | denotes | of |
T8102 | 1384-1387 | DT | denotes | the |
T8103 | 1388-1401 | NN | denotes | concentration |
T8104 | 1402-1404 | IN | denotes | of |
T8105 | 1405-1416 | JJ | denotes | recombinant |
T8106 | 1417-1426 | NNS | denotes | cytokines |
T8107 | 1426-1427 | , | denotes | , |
T8108 | 1428-1431 | CC | denotes | and |
T8109 | 1432-1436 | VBN | denotes | used |
T8110 | 1437-1440 | IN | denotes | for |
T8111 | 1441-1454 | NN | denotes | determination |
T8112 | 1455-1457 | IN | denotes | of |
T8113 | 1458-1463 | NNP | denotes | IL-17 |
T8114 | 1464-1466 | IN | denotes | in |
T8115 | 1467-1471 | NN | denotes | test |
T8116 | 1472-1479 | NNS | denotes | samples |
T8117 | 1479-1480 | . | denotes | . |
R4194 | T7843 | T7845 | amod | Enzyme-linked,assay |
R4195 | T7844 | T7845 | amod | immunosorbent,assay |
R4196 | T7845 | T7853 | nsubjpass | assay,measured |
R4197 | T7846 | T7845 | prep | of,assay |
R4198 | T7847 | T7848 | compound | IL-17,IL-17 |
R4199 | T7848 | T7846 | pobj | IL-17,of |
R4200 | T7849 | T7845 | prep | in,assay |
R4201 | T7850 | T7851 | compound | culture,supernatants |
R4202 | T7851 | T7849 | pobj | supernatants,in |
R4203 | T7852 | T7853 | auxpass | was,measured |
R4204 | T7853 | T7853 | ROOT | measured,measured |
R4205 | T7854 | T7853 | agent | by,measured |
R4206 | T7855 | T7858 | nmod | sandwich,assay |
R4207 | T7856 | T7858 | amod | enzyme-linked,assay |
R4208 | T7857 | T7858 | compound | immunosorbent,assay |
R4209 | T7858 | T7854 | pobj | assay,by |
R4210 | T7859 | T7860 | mark | as,described |
R4211 | T7860 | T7853 | advcl | described,measured |
R4212 | T7861 | T7864 | advmod | previously,] |
R4213 | T7862 | T7864 | nmod | [,] |
R4214 | T7863 | T7864 | nummod | 20,] |
R4215 | T7864 | T7860 | dobj | ],described |
R4216 | T7865 | T7853 | punct | .,measured |
R4217 | T7866 | T7876 | prep | In,coated |
R4218 | T7867 | T7866 | pobj | brief,In |
R4219 | T7868 | T7876 | punct | ",",coated |
R4220 | T7869 | T7871 | det | a,plate |
R4221 | T7870 | T7871 | amod | 96-well,plate |
R4222 | T7871 | T7876 | nsubjpass | plate,coated |
R4223 | T7872 | T7873 | punct | (,Nunc |
R4224 | T7873 | T7871 | appos | Nunc,plate |
R4225 | T7874 | T7873 | punct | ),Nunc |
R4226 | T7875 | T7876 | auxpass | was,coated |
R4227 | T7876 | T7876 | ROOT | coated,coated |
R4228 | T7877 | T7876 | prep | with,coated |
R4229 | T7878 | T7879 | nummod | 4,μg |
R4230 | T7879 | T7883 | amod | μg,antibodies |
R4231 | T7880 | T7883 | nmod | /,antibodies |
R4232 | T7881 | T7883 | amod | ml,antibodies |
R4233 | T7882 | T7883 | amod | monoclonal,antibodies |
R4234 | T7883 | T7877 | pobj | antibodies,with |
R4235 | T7884 | T7883 | prep | against,antibodies |
R4236 | T7885 | T7884 | pobj | IL-17,against |
R4237 | T7886 | T7885 | punct | (,IL-17 |
R4238 | T7887 | T7885 | conj | R,IL-17 |
R4239 | T7888 | T7887 | cc | &,R |
R4240 | T7889 | T7890 | compound | D,Systems |
R4241 | T7890 | T7887 | conj | Systems,R |
R4242 | T7891 | T7885 | punct | ),IL-17 |
R4243 | T7892 | T7876 | prep | at,coated |
R4244 | T7893 | T7894 | nummod | 4,° |
R4245 | T7894 | T7895 | nummod | °,C |
R4246 | T7895 | T7896 | compound | C,overnight |
R4247 | T7896 | T7892 | pobj | overnight,at |
R4248 | T7897 | T7876 | punct | .,coated |
R4249 | T7898 | T7910 | prep | After,/ |
R4250 | T7899 | T7898 | pcomp | blocking,After |
R4251 | T7900 | T7899 | prep | with,blocking |
R4252 | T7901 | T7906 | amod | phosphate-buffered,albumin |
R4253 | T7902 | T7903 | nummod | saline/1,% |
R4254 | T7903 | T7906 | compound | %,albumin |
R4255 | T7904 | T7905 | amod | bovine,serum |
R4256 | T7905 | T7906 | compound | serum,albumin |
R4257 | T7906 | T7900 | pobj | albumin,with |
R4258 | T7907 | T7908 | punct | (,BSA |
R4259 | T7908 | T7906 | npadvmod | BSA,albumin |
R4260 | T7909 | T7910 | punct | ),/ |
R4261 | T7910 | T7910 | ROOT | /,/ |
R4262 | T7911 | T7912 | nummod | 0.05,% |
R4263 | T7912 | T7929 | nmod | %,samples |
R4264 | T7913 | T7914 | nummod | Tween,20 |
R4265 | T7914 | T7929 | nummod | 20,samples |
R4266 | T7915 | T7914 | prep | for,20 |
R4267 | T7916 | T7917 | nummod | 2,hours |
R4268 | T7917 | T7915 | pobj | hours,for |
R4269 | T7918 | T7917 | prep | at,hours |
R4270 | T7919 | T7920 | compound | room,temperature |
R4271 | T7920 | T7918 | pobj | temperature,at |
R4272 | T7921 | T7917 | punct | (,hours |
R4273 | T7922 | T7917 | nummod | 22,hours |
R4274 | T7923 | T7925 | nummod | 25,C |
R4275 | T7924 | T7925 | compound | °,C |
R4276 | T7925 | T7917 | appos | C,hours |
R4277 | T7926 | T7914 | punct | ),20 |
R4278 | T7927 | T7914 | punct | ",",20 |
R4279 | T7928 | T7929 | compound | test,samples |
R4280 | T7929 | T7910 | dobj | samples,/ |
R4281 | T7930 | T7929 | cc | and,samples |
R4282 | T7931 | T7934 | det | the,IL-17 |
R4283 | T7932 | T7934 | amod | standard,IL-17 |
R4284 | T7933 | T7934 | compound | recombinant,IL-17 |
R4285 | T7934 | T7929 | conj | IL-17,samples |
R4286 | T7935 | T7942 | punct | (,added |
R4287 | T7936 | T7942 | nsubjpass | R,added |
R4288 | T7937 | T7936 | cc | &,R |
R4289 | T7938 | T7939 | compound | D,Systems |
R4290 | T7939 | T7936 | conj | Systems,R |
R4291 | T7940 | T7942 | punct | ),added |
R4292 | T7941 | T7942 | auxpass | were,added |
R4293 | T7942 | T7934 | parataxis | added,IL-17 |
R4294 | T7943 | T7942 | prep | to,added |
R4295 | T7944 | T7946 | det | the,plate |
R4296 | T7945 | T7946 | amod | 96-well,plate |
R4297 | T7946 | T7943 | pobj | plate,to |
R4298 | T7947 | T7942 | cc | and,added |
R4299 | T7948 | T7942 | conj | incubated,added |
R4300 | T7949 | T7948 | prep | at,incubated |
R4301 | T7950 | T7951 | compound | room,temperature |
R4302 | T7951 | T7949 | pobj | temperature,at |
R4303 | T7952 | T7948 | prep | for,incubated |
R4304 | T7953 | T7954 | nummod | 2,hours |
R4305 | T7954 | T7952 | pobj | hours,for |
R4306 | T7955 | T7942 | punct | .,added |
R4307 | T7956 | T7958 | nsubjpass | Plates,washed |
R4308 | T7957 | T7958 | auxpass | were,washed |
R4309 | T7958 | T7958 | ROOT | washed,washed |
R4310 | T7959 | T7960 | nummod | four,times |
R4311 | T7960 | T7958 | npadvmod | times,washed |
R4312 | T7961 | T7958 | prep | with,washed |
R4313 | T7962 | T7963 | compound | phosphate-buffered,saline/Tween |
R4314 | T7963 | T7961 | pobj | saline/Tween,with |
R4315 | T7964 | T7963 | nummod | 20,saline/Tween |
R4316 | T7965 | T7958 | punct | ",",washed |
R4317 | T7966 | T7958 | cc | and,washed |
R4318 | T7967 | T7968 | advmod | then,incubated |
R4319 | T7968 | T7958 | conj | incubated,washed |
R4320 | T7969 | T7968 | prep | with,incubated |
R4321 | T7970 | T7975 | nummod | 500,antibodies |
R4322 | T7971 | T7975 | amod | ng/ml,antibodies |
R4323 | T7972 | T7975 | amod | biotinylated,antibodies |
R4324 | T7973 | T7975 | compound | mouse,antibodies |
R4325 | T7974 | T7975 | compound | monoclonal,antibodies |
R4326 | T7975 | T7969 | pobj | antibodies,with |
R4327 | T7976 | T7975 | prep | against,antibodies |
R4328 | T7977 | T7976 | pobj | IL-17,against |
R4329 | T7978 | T7977 | punct | (,IL-17 |
R4330 | T7979 | T7977 | conj | R,IL-17 |
R4331 | T7980 | T7979 | cc | &,R |
R4332 | T7981 | T7982 | compound | D,Systems |
R4333 | T7982 | T7979 | conj | Systems,R |
R4334 | T7983 | T7968 | punct | ),incubated |
R4335 | T7984 | T7968 | prep | for,incubated |
R4336 | T7985 | T7986 | nummod | 2,hours |
R4337 | T7986 | T7984 | pobj | hours,for |
R4338 | T7987 | T7986 | prep | at,hours |
R4339 | T7988 | T7989 | compound | room,temperature |
R4340 | T7989 | T7987 | pobj | temperature,at |
R4341 | T7990 | T7958 | punct | .,washed |
R4342 | T7991 | T8004 | prep | After,incubated |
R4343 | T7992 | T7991 | pcomp | washing,After |
R4344 | T7993 | T8004 | punct | ",",incubated |
R4345 | T7994 | T8004 | nsubjpass | streptavidin,incubated |
R4346 | T7995 | T7996 | amod | alkaline,phosphate |
R4347 | T7996 | T8003 | nsubj | phosphate,was |
R4348 | T7997 | T7999 | amod | horseradish,conjugate |
R4349 | T7998 | T7999 | amod | peroxidase,conjugate |
R4350 | T7999 | T7996 | appos | conjugate,phosphate |
R4351 | T8000 | T8001 | punct | (,Sigma |
R4352 | T8001 | T7999 | appos | Sigma,conjugate |
R4353 | T8002 | T7999 | punct | ),conjugate |
R4354 | T8003 | T8004 | auxpass | was,incubated |
R4355 | T8004 | T8004 | ROOT | incubated,incubated |
R4356 | T8005 | T8004 | prep | for,incubated |
R4357 | T8006 | T8007 | nummod | 2,hours |
R4358 | T8007 | T8005 | pobj | hours,for |
R4359 | T8008 | T8010 | punct | ",",washed |
R4360 | T8009 | T8010 | advmod | then,washed |
R4361 | T8010 | T8004 | dep | washed,incubated |
R4362 | T8011 | T8010 | advmod | again,washed |
R4363 | T8012 | T8010 | cc | and,washed |
R4364 | T8013 | T8010 | conj | incubated,washed |
R4365 | T8014 | T8013 | prep | with,incubated |
R4366 | T8015 | T8016 | nummod | 1,mg/ml |
R4367 | T8016 | T8018 | nmod | mg/ml,phosphate |
R4368 | T8017 | T8018 | compound | p-nitrophenyl,phosphate |
R4369 | T8018 | T8014 | pobj | phosphate,with |
R4370 | T8019 | T8020 | punct | (,Sigma |
R4371 | T8020 | T8018 | appos | Sigma,phosphate |
R4372 | T8021 | T8013 | punct | ),incubated |
R4373 | T8022 | T8010 | conj | dissolved,washed |
R4374 | T8023 | T8022 | prep | in,dissolved |
R4375 | T8024 | T8023 | pobj | diethanolamine,in |
R4376 | T8025 | T8026 | punct | (,Sigma |
R4377 | T8026 | T8024 | appos | Sigma,diethanolamine |
R4378 | T8027 | T8024 | punct | ),diethanolamine |
R4379 | T8028 | T8029 | aux | to,develop |
R4380 | T8029 | T8022 | advcl | develop,dissolved |
R4381 | T8030 | T8032 | det | the,reaction |
R4382 | T8031 | T8032 | compound | color,reaction |
R4383 | T8032 | T8029 | dobj | reaction,develop |
R4384 | T8033 | T8004 | punct | .,incubated |
R4385 | T8034 | T8035 | det | The,reaction |
R4386 | T8035 | T8037 | nsubjpass | reaction,stopped |
R4387 | T8036 | T8037 | auxpass | was,stopped |
R4388 | T8037 | T8037 | ROOT | stopped,stopped |
R4389 | T8038 | T8037 | agent | by,stopped |
R4390 | T8039 | T8040 | det | the,addition |
R4391 | T8040 | T8038 | pobj | addition,by |
R4392 | T8041 | T8040 | prep | of,addition |
R4393 | T8042 | T8044 | nummod | 1,NaOH |
R4394 | T8043 | T8044 | compound | M,NaOH |
R4395 | T8044 | T8041 | pobj | NaOH,of |
R4396 | T8045 | T8044 | cc | and,NaOH |
R4397 | T8046 | T8048 | det | the,density |
R4398 | T8047 | T8048 | amod | optical,density |
R4399 | T8048 | T8044 | conj | density,NaOH |
R4400 | T8049 | T8048 | prep | of,density |
R4401 | T8050 | T8051 | det | each,well |
R4402 | T8051 | T8049 | pobj | well,of |
R4403 | T8052 | T8053 | auxpass | was,read |
R4404 | T8053 | T8037 | conj | read,stopped |
R4405 | T8054 | T8053 | prep | at,read |
R4406 | T8055 | T8056 | nummod | 405,nm |
R4407 | T8056 | T8054 | pobj | nm,at |
R4408 | T8057 | T8053 | punct | .,read |
R4409 | T8058 | T8060 | det | The,limit |
R4410 | T8059 | T8060 | amod | lower,limit |
R4411 | T8060 | T8064 | nsubj | limit,was |
R4412 | T8061 | T8060 | prep | of,limit |
R4413 | T8062 | T8063 | compound | IL-17,detection |
R4414 | T8063 | T8061 | pobj | detection,of |
R4415 | T8064 | T8064 | ROOT | was,was |
R4416 | T8065 | T8066 | nummod | 10,pg/ml |
R4417 | T8066 | T8064 | attr | pg/ml,was |
R4418 | T8067 | T8064 | punct | .,was |
R4419 | T8068 | T8070 | amod | Recombinant,IL-17 |
R4420 | T8069 | T8070 | amod | human,IL-17 |
R4421 | T8070 | T8071 | nsubj | IL-17,diluted |
R4422 | T8071 | T8076 | nsubjpass | diluted,used |
R4423 | T8072 | T8071 | prep | in,diluted |
R4424 | T8073 | T8074 | compound | culture,medium |
R4425 | T8074 | T8072 | pobj | medium,in |
R4426 | T8075 | T8076 | auxpass | was,used |
R4427 | T8076 | T8076 | ROOT | used,used |
R4428 | T8077 | T8076 | prep | as,used |
R4429 | T8078 | T8080 | det | a,standard |
R4430 | T8079 | T8080 | compound | calibration,standard |
R4431 | T8080 | T8077 | pobj | standard,as |
R4432 | T8081 | T8076 | punct | ",",used |
R4433 | T8082 | T8076 | advcl | ranging,used |
R4434 | T8083 | T8082 | prep | from,ranging |
R4435 | T8084 | T8083 | pobj | 10,from |
R4436 | T8085 | T8083 | prep | to,from |
R4437 | T8086 | T8087 | det | 2000,pg/ml |
R4438 | T8087 | T8085 | pobj | pg/ml,to |
R4439 | T8088 | T8076 | punct | .,used |
R4440 | T8089 | T8091 | det | A,curve |
R4441 | T8090 | T8091 | amod | standard,curve |
R4442 | T8091 | T8093 | nsubjpass | curve,drawn |
R4443 | T8092 | T8093 | auxpass | was,drawn |
R4444 | T8093 | T8093 | ROOT | drawn,drawn |
R4445 | T8094 | T8093 | agent | by,drawn |
R4446 | T8095 | T8094 | pcomp | plotting,by |
R4447 | T8096 | T8097 | amod | optical,density |
R4448 | T8097 | T8095 | dobj | density,plotting |
R4449 | T8098 | T8097 | prep | against,density |
R4450 | T8099 | T8100 | det | the,log |
R4451 | T8100 | T8098 | pobj | log,against |
R4452 | T8101 | T8100 | prep | of,log |
R4453 | T8102 | T8103 | det | the,concentration |
R4454 | T8103 | T8101 | pobj | concentration,of |
R4455 | T8104 | T8103 | prep | of,concentration |
R4456 | T8105 | T8106 | amod | recombinant,cytokines |
R4457 | T8106 | T8104 | pobj | cytokines,of |
R4458 | T8107 | T8093 | punct | ",",drawn |
R4459 | T8108 | T8093 | cc | and,drawn |
R4460 | T8109 | T8093 | conj | used,drawn |
R4461 | T8110 | T8109 | prep | for,used |
R4462 | T8111 | T8110 | pobj | determination,for |
R4463 | T8112 | T8111 | prep | of,determination |
R4464 | T8113 | T8112 | pobj | IL-17,of |
R4465 | T8114 | T8111 | prep | in,determination |
R4466 | T8115 | T8116 | compound | test,samples |
R4467 | T8116 | T8114 | pobj | samples,in |
R4468 | T8117 | T8093 | punct | .,drawn |