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PMC:1064895 / 5649-15482 JSONTXT 26 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5038 23-31 NNS denotes Patients
T5039 32-40 VBN denotes Informed
T5040 41-48 NN denotes consent
T5041 49-52 VBD denotes was
T5042 53-61 VBN denotes obtained
T5043 62-66 IN denotes from
T5044 67-69 CD denotes 24
T5045 70-78 NNS denotes patients
T5046 79-80 -LRB- denotes (
T5047 80-81 CD denotes 5
T5048 82-85 NNS denotes men
T5049 86-89 CC denotes and
T5050 90-92 CD denotes 19
T5051 93-98 NNS denotes women
T5052 98-99 -RRB- denotes )
T5053 100-104 IN denotes with
T5054 105-107 NNP denotes RA
T5055 108-111 WP denotes who
T5056 112-121 VBD denotes fulfilled
T5057 122-125 DT denotes the
T5058 126-130 CD denotes 1987
T5059 131-138 VBN denotes revised
T5060 139-147 NNS denotes criteria
T5061 148-150 IN denotes of
T5062 151-154 DT denotes the
T5063 155-163 JJ denotes American
T5064 164-171 NNP denotes College
T5065 172-174 IN denotes of
T5066 175-187 NNP denotes Rheumatology
T5067 188-189 -LRB- denotes (
T5068 189-197 RB denotes formerly
T5069 198-201 DT denotes the
T5070 202-210 JJ denotes American
T5071 211-221 NNP denotes Rheumatism
T5072 222-233 NNP denotes Association
T5073 233-234 -RRB- denotes )
T5074 235-236 NNP denotes [
T5075 236-238 CD denotes 17
T5076 238-239 NNP denotes ]
T5077 239-240 . denotes .
T5078 241-244 DT denotes The
T5079 245-248 NN denotes age
T5080 249-251 IN denotes of
T5081 252-255 DT denotes the
T5082 256-264 NNS denotes patients
T5083 265-269 IN denotes with
T5084 270-272 NNP denotes RA
T5085 273-276 VBD denotes was
T5086 277-279 CD denotes 50
T5087 280-281 NN denotes ±
T5088 282-283 CD denotes 8
T5089 284-285 -LRB- denotes (
T5090 285-289 JJ denotes mean
T5091 290-291 NN denotes ±
T5092 292-295 NNP denotes SEM
T5093 295-296 -RRB- denotes )
T5094 297-302 NNS denotes years
T5095 303-304 -LRB- denotes (
T5096 304-309 NN denotes range
T5097 310-312 CD denotes 23
T5098 313-315 CD denotes 71
T5099 316-321 NNS denotes years
T5100 321-322 -RRB- denotes )
T5101 322-323 . denotes .
T5102 324-327 DT denotes All
T5103 328-339 NNS denotes medications
T5104 340-344 VBD denotes were
T5105 345-352 VBN denotes stopped
T5106 353-355 CD denotes 48
T5107 356-361 NNS denotes hours
T5108 362-368 IN denotes before
T5109 369-374 NN denotes entry
T5110 375-377 TO denotes to
T5111 378-381 DT denotes the
T5112 382-387 NN denotes study
T5113 387-388 . denotes .
T5114 389-400 NNS denotes Comparisons
T5115 401-405 VBD denotes were
T5116 406-410 VBN denotes made
T5117 411-415 IN denotes with
T5118 416-418 CD denotes 14
T5119 419-427 NNS denotes patients
T5120 428-432 IN denotes with
T5121 433-435 NNP denotes OA
T5122 436-437 -LRB- denotes (
T5123 437-438 CD denotes 3
T5124 439-442 NNS denotes men
T5125 443-446 CC denotes and
T5126 447-449 CD denotes 11
T5127 450-455 NNS denotes women
T5128 455-456 -RRB- denotes )
T5129 457-460 CC denotes and
T5130 461-465 IN denotes with
T5131 466-468 CD denotes 14
T5132 469-476 JJ denotes healthy
T5133 477-485 NNS denotes controls
T5134 486-487 -LRB- denotes (
T5135 487-488 CD denotes 3
T5136 489-492 NNS denotes men
T5137 493-496 CC denotes and
T5138 497-499 CD denotes 11
T5139 500-505 NNS denotes women
T5140 505-506 -RRB- denotes )
T5141 507-510 WP denotes who
T5142 511-514 VBD denotes had
T5143 515-517 DT denotes no
T5144 518-527 JJ denotes rheumatic
T5145 528-536 NNS denotes diseases
T5146 536-537 . denotes .
T5147 538-541 DT denotes The
T5148 542-546 JJ denotes mean
T5149 547-551 NNS denotes ages
T5150 552-554 IN denotes of
T5151 555-558 DT denotes the
T5152 559-567 NNS denotes patients
T5153 568-572 IN denotes with
T5154 573-575 NNP denotes OA
T5155 576-579 CC denotes and
T5156 580-583 DT denotes the
T5157 584-591 JJ denotes healthy
T5158 592-600 NNS denotes controls
T5159 601-605 VBD denotes were
T5160 606-608 CD denotes 50
T5161 609-610 NN denotes ±
T5162 611-612 CD denotes 8
T5163 613-618 NNS denotes years
T5164 619-620 -LRB- denotes (
T5165 620-625 NN denotes range
T5166 626-628 CD denotes 34
T5167 629-631 CD denotes 68
T5168 632-637 NNS denotes years
T5169 637-638 -RRB- denotes )
T5170 639-642 CC denotes and
T5171 643-645 CD denotes 30
T5172 646-647 NN denotes ±
T5173 648-649 CD denotes 6
T5174 650-655 NNS denotes years
T5175 656-657 -LRB- denotes (
T5176 657-662 NN denotes range
T5177 663-665 CD denotes 24
T5178 666-668 CD denotes 57
T5179 669-674 NNS denotes years
T5180 674-675 -RRB- denotes )
T5181 675-676 . denotes .
T5182 677-685 VBN denotes Informed
T5183 686-693 NN denotes consent
T5184 694-697 VBD denotes was
T5185 698-706 VBN denotes obtained
T5186 706-707 , denotes ,
T5187 708-711 CC denotes and
T5188 712-715 DT denotes the
T5189 716-724 NN denotes protocol
T5190 725-728 VBD denotes was
T5191 729-737 VBN denotes approved
T5192 738-740 IN denotes by
T5193 741-744 DT denotes the
T5194 745-753 NNP denotes Catholic
T5195 754-764 NNP denotes University
T5196 765-767 IN denotes of
T5197 768-773 NNP denotes Korea
T5198 774-779 NNP denotes Human
T5199 780-788 NNP denotes Research
T5200 789-795 NNP denotes Ethics
T5201 796-805 NNP denotes Committee
T5202 805-806 . denotes .
T5665 808-816 NNP denotes Reagents
T5666 817-828 NNP denotes Recombinant
T5667 829-834 NNP denotes IL-17
T5668 834-835 , denotes ,
T5669 836-841 NNP denotes IL-18
T5670 841-842 , denotes ,
T5671 843-848 NNP denotes IL-15
T5672 848-849 , denotes ,
T5673 850-858 NN denotes monocyte
T5674 859-874 NN denotes chemoattractant
T5675 875-884 NN denotes protein-1
T5676 885-886 -LRB- denotes (
T5677 886-891 NN denotes MCP-1
T5678 891-892 -RRB- denotes )
T5679 892-893 , denotes ,
T5680 894-904 NN denotes macrophage
T5681 905-917 JJ denotes inflammatory
T5682 918-925 NN denotes protein
T5683 926-927 -LRB- denotes (
T5684 927-930 NNP denotes MIP
T5685 930-931 -RRB- denotes )
T5686 931-933 CD denotes -1
T5687 933-934 NN denotes α
T5688 934-935 , denotes ,
T5689 936-942 NNP denotes MIP-1β
T5690 942-943 , denotes ,
T5691 944-948 NNP denotes IL-6
T5692 949-952 CC denotes and
T5693 953-957 NNP denotes IL-8
T5694 958-962 VBD denotes were
T5695 963-972 VBN denotes purchased
T5696 973-977 IN denotes from
T5697 978-979 NN denotes R
T5698 980-981 CC denotes &
T5699 982-983 NNP denotes D
T5700 984-991 NNS denotes systems
T5701 992-993 -LRB- denotes (
T5702 993-1004 NNP denotes Minneapolis
T5703 1004-1005 , denotes ,
T5704 1006-1008 NNP denotes MN
T5705 1008-1009 , denotes ,
T5706 1010-1013 NNP denotes USA
T5707 1013-1014 -RRB- denotes )
T5708 1014-1015 . denotes .
T5709 1016-1027 JJ denotes Recombinant
T5710 1028-1040 VBG denotes transforming
T5711 1041-1047 NN denotes growth
T5712 1048-1054 NN denotes factor
T5713 1055-1056 -LRB- denotes (
T5714 1056-1059 NNP denotes TGF
T5715 1059-1060 -RRB- denotes )
T5716 1060-1061 : denotes -
T5717 1061-1062 NN denotes β
T5718 1063-1066 VBD denotes was
T5719 1067-1076 VBN denotes purchased
T5720 1077-1081 IN denotes from
T5721 1082-1091 NNP denotes Peprotech
T5722 1092-1093 -LRB- denotes (
T5723 1093-1099 NNP denotes London
T5724 1099-1100 , denotes ,
T5725 1101-1103 NNP denotes UK
T5726 1103-1104 -RRB- denotes )
T5727 1104-1105 . denotes .
T5728 1106-1117 JJ denotes Recombinant
T5729 1118-1123 NN denotes TNF-α
T5730 1124-1127 CC denotes and
T5731 1128-1132 NN denotes IL-1
T5732 1133-1137 VBD denotes were
T5733 1138-1147 VBN denotes purchased
T5734 1148-1152 IN denotes from
T5735 1153-1160 NNP denotes Endogen
T5736 1161-1165 NNP denotes Inc.
T5737 1166-1167 -LRB- denotes (
T5738 1167-1176 NNP denotes Cambridge
T5739 1176-1177 , denotes ,
T5740 1178-1180 NNP denotes MA
T5741 1180-1181 , denotes ,
T5742 1182-1185 NNP denotes USA
T5743 1185-1186 -RRB- denotes )
T5744 1186-1187 . denotes .
T5745 1188-1199 NNP denotes Cyclosporin
T5746 1200-1201 NNP denotes A
T5747 1202-1205 VBD denotes was
T5748 1206-1214 VBN denotes provided
T5749 1215-1217 IN denotes by
T5750 1218-1224 NNP denotes Sandos
T5751 1225-1229 NNP denotes Ltd.
T5752 1230-1231 -LRB- denotes (
T5753 1231-1236 NNP denotes Basel
T5754 1236-1237 , denotes ,
T5755 1238-1249 NNP denotes Switzerland
T5756 1249-1250 -RRB- denotes )
T5757 1250-1251 . denotes .
T5758 1252-1270 NNP denotes Phytohemagglutinin
T5759 1271-1272 -LRB- denotes (
T5760 1272-1275 NNP denotes PHA
T5761 1275-1276 -RRB- denotes )
T5762 1276-1277 , denotes ,
T5763 1278-1289 JJ denotes pyrrolidine
T5764 1290-1305 NN denotes dithiocarbamate
T5765 1306-1307 -LRB- denotes (
T5766 1307-1311 NNP denotes PDTC
T5767 1311-1312 -RRB- denotes )
T5768 1312-1313 , denotes ,
T5769 1314-1323 NN denotes rapamycin
T5770 1323-1324 , denotes ,
T5771 1325-1338 NN denotes dexamethasone
T5772 1339-1342 CC denotes and
T5773 1343-1351 NN denotes curcumin
T5774 1352-1356 VBD denotes were
T5775 1357-1360 DT denotes all
T5776 1361-1369 VBN denotes obtained
T5777 1370-1374 IN denotes from
T5778 1375-1378 DT denotes the
T5779 1379-1384 NNP denotes Sigma
T5780 1385-1393 NNP denotes Chemical
T5781 1394-1397 NNP denotes Co.
T5782 1398-1399 -LRB- denotes (
T5783 1399-1401 NNP denotes St
T5784 1402-1407 NNP denotes Louis
T5785 1407-1408 , denotes ,
T5786 1409-1411 NNP denotes MA
T5787 1411-1412 , denotes ,
T5788 1413-1416 NNP denotes USA
T5789 1416-1417 -RRB- denotes )
T5790 1417-1418 . denotes .
T5791 1419-1427 JJ denotes Anti-CD3
T5792 1428-1438 JJ denotes monoclonal
T5793 1439-1447 NN denotes antibody
T5794 1448-1451 CC denotes and
T5795 1452-1461 JJ denotes anti-CD28
T5796 1462-1472 JJ denotes monoclonal
T5797 1473-1481 NN denotes antibody
T5798 1482-1486 VBD denotes were
T5799 1487-1495 VBN denotes obtained
T5800 1496-1500 IN denotes from
T5801 1501-1503 NNP denotes BD
T5802 1504-1515 NNP denotes Biosciences
T5803 1516-1517 -LRB- denotes (
T5804 1517-1520 NNP denotes San
T5805 1521-1526 NNP denotes Diego
T5806 1526-1527 , denotes ,
T5807 1528-1530 NNP denotes CA
T5808 1530-1531 , denotes ,
T5809 1532-1535 NNP denotes USA
T5810 1535-1536 -RRB- denotes )
T5811 1536-1537 . denotes .
T5812 1538-1546 CD denotes LY294002
T5813 1546-1547 , denotes ,
T5814 1548-1556 NNP denotes SB203580
T5815 1556-1557 , denotes ,
T5816 1558-1563 NNP denotes FK506
T5817 1563-1564 , denotes ,
T5818 1565-1575 NN denotes wortmannin
T5819 1576-1579 CC denotes and
T5820 1580-1587 CD denotes PD98059
T5821 1588-1592 VBD denotes were
T5822 1593-1601 VBN denotes obtained
T5823 1602-1606 IN denotes from
T5824 1607-1617 NNP denotes Calbiochem
T5825 1618-1619 -LRB- denotes (
T5826 1619-1629 NNP denotes Schwalbach
T5827 1629-1630 , denotes ,
T5828 1631-1638 NNP denotes Germany
T5829 1638-1639 -RRB- denotes )
T5830 1639-1640 . denotes .
T6294 1642-1652 NN denotes Production
T6295 1653-1655 IN denotes of
T6296 1656-1661 NNP denotes IL-17
T6297 1662-1664 IN denotes by
T6298 1665-1666 NNP denotes T
T6299 1667-1671 NN denotes cell
T6300 1672-1680 NN denotes receptor
T6301 1681-1691 NN denotes activation
T6302 1691-1692 , denotes ,
T6303 1693-1702 NNS denotes cytokines
T6304 1703-1705 CC denotes or
T6305 1706-1716 NNS denotes chemokines
T6306 1717-1721 NNP denotes PBMC
T6307 1722-1726 VBD denotes were
T6308 1727-1735 VBN denotes prepared
T6309 1736-1740 IN denotes from
T6310 1741-1752 VBN denotes heparinized
T6311 1753-1758 NN denotes blood
T6312 1759-1761 IN denotes by
T6313 1762-1776 NNP denotes Ficoll-Hypaque
T6314 1777-1778 -LRB- denotes (
T6315 1778-1784 NNP denotes SG1077
T6316 1784-1785 -RRB- denotes )
T6317 1786-1802 JJ denotes density-gradient
T6318 1803-1817 NN denotes centrifugation
T6319 1817-1818 . denotes .
T6320 1819-1823 NNP denotes Cell
T6321 1824-1832 NNS denotes cultures
T6322 1833-1837 VBD denotes were
T6323 1838-1847 VBN denotes performed
T6324 1848-1850 IN denotes as
T6325 1851-1860 VBN denotes described
T6326 1861-1871 RB denotes previously
T6327 1872-1873 NNP denotes [
T6328 1873-1875 CD denotes 18
T6329 1875-1876 NNP denotes ]
T6330 1876-1877 . denotes .
T6331 1878-1880 IN denotes In
T6332 1881-1886 NN denotes brief
T6333 1886-1887 , denotes ,
T6334 1888-1891 DT denotes the
T6335 1892-1896 NN denotes cell
T6336 1897-1908 NNS denotes suspensions
T6337 1909-1913 VBD denotes were
T6338 1914-1922 VBN denotes adjusted
T6339 1923-1925 TO denotes to
T6340 1926-1927 DT denotes a
T6341 1928-1941 NN denotes concentration
T6342 1942-1944 IN denotes of
T6343 1945-1951 CD denotes 106/ml
T6344 1952-1954 IN denotes in
T6345 1955-1959 NNP denotes RPMI
T6346 1960-1964 CD denotes 1640
T6347 1965-1971 JJ denotes medium
T6348 1972-1984 VBN denotes supplemented
T6349 1985-1989 IN denotes with
T6350 1990-1992 CD denotes 10
T6351 1992-1993 NN denotes %
T6352 1994-1999 JJ denotes fetal
T6353 2000-2004 NN denotes calf
T6354 2005-2010 NN denotes serum
T6355 2010-2011 , denotes ,
T6356 2012-2015 CD denotes 100
T6357 2016-2020 NNP denotes U/ml
T6358 2021-2031 NN denotes penicillin
T6359 2031-2032 , denotes ,
T6360 2033-2036 CD denotes 100
T6361 2037-2042 JJ denotes mg/ml
T6362 2043-2055 NN denotes streptomycin
T6363 2056-2059 CC denotes and
T6364 2060-2061 CD denotes 2
T6365 2062-2064 NNP denotes mM
T6366 2065-2076 NNP denotes L-glutamine
T6367 2076-2077 . denotes .
T6368 2078-2082 NNP denotes Cell
T6369 2083-2093 NN denotes suspension
T6370 2094-2095 -LRB- denotes (
T6371 2095-2096 CD denotes 1
T6372 2097-2099 NN denotes ml
T6373 2099-2100 -RRB- denotes )
T6374 2101-2104 VBD denotes was
T6375 2105-2114 VBN denotes dispensed
T6376 2115-2119 IN denotes into
T6377 2120-2127 JJ denotes 24-well
T6378 2128-2138 JJ denotes multi-well
T6379 2139-2145 NNS denotes plates
T6380 2146-2147 -LRB- denotes (
T6381 2147-2151 NNP denotes Nunc
T6382 2151-2152 , denotes ,
T6383 2153-2161 NNP denotes Roskilde
T6384 2161-2162 , denotes ,
T6385 2163-2170 NNP denotes Denmark
T6386 2170-2171 -RRB- denotes )
T6387 2171-2172 , denotes ,
T6388 2173-2176 CC denotes and
T6389 2177-2186 VBD denotes incubated
T6390 2187-2190 IN denotes for
T6391 2191-2193 CD denotes 24
T6392 2194-2199 NNS denotes hours
T6393 2200-2202 IN denotes at
T6394 2203-2205 CD denotes 37
T6395 2205-2206 CD denotes °
T6396 2206-2207 NNP denotes C
T6397 2208-2210 IN denotes in
T6398 2211-2212 CD denotes 5
T6399 2212-2213 NN denotes %
T6400 2214-2217 CD denotes CO2
T6401 2217-2218 . denotes .
T6402 2219-2231 RB denotes Subsequently
T6403 2231-2232 , denotes ,
T6404 2233-2240 JJ denotes various
T6405 2241-2255 NNS denotes concentrations
T6406 2256-2258 IN denotes of
T6407 2259-2270 NN denotes cyclosporin
T6408 2271-2272 DT denotes A
T6409 2273-2274 -LRB- denotes (
T6410 2274-2276 CD denotes 10
T6411 2277-2280 CD denotes 500
T6412 2281-2286 NN denotes ng/ml
T6413 2286-2287 -RRB- denotes )
T6414 2288-2292 VBD denotes were
T6415 2293-2298 VBN denotes added
T6416 2299-2301 TO denotes to
T6417 2302-2305 DT denotes the
T6418 2306-2312 NN denotes medium
T6419 2313-2316 CC denotes and
T6420 2317-2322 NNS denotes cells
T6421 2323-2327 VBD denotes were
T6422 2328-2337 VBN denotes incubated
T6423 2338-2341 IN denotes for
T6424 2342-2344 CD denotes 24
T6425 2345-2350 NNS denotes hours
T6426 2350-2351 . denotes .
T6427 2352-2354 TO denotes To
T6428 2355-2359 DT denotes each
T6429 2360-2364 NN denotes well
T6430 2365-2368 VBD denotes was
T6431 2369-2374 VBN denotes added
T6432 2375-2380 NNP denotes FK506
T6433 2380-2381 , denotes ,
T6434 2382-2391 NN denotes rapamycin
T6435 2391-2392 , denotes ,
T6436 2393-2401 NN denotes curcumin
T6437 2401-2402 , denotes ,
T6438 2403-2407 NNP denotes PDTC
T6439 2407-2408 , denotes ,
T6440 2409-2417 NNP denotes LY294002
T6441 2417-2418 , denotes ,
T6442 2419-2427 NNP denotes SB203580
T6443 2427-2428 , denotes ,
T6444 2429-2436 NNP denotes PD98059
T6445 2436-2437 , denotes ,
T6446 2438-2451 NN denotes dexamethasone
T6447 2452-2454 CC denotes or
T6448 2455-2465 NN denotes wortmannin
T6449 2465-2466 . denotes .
T6450 2467-2472 IN denotes After
T6451 2473-2483 NN denotes incubation
T6452 2484-2487 IN denotes for
T6453 2488-2490 CD denotes 24
T6454 2491-2496 NNS denotes hours
T6455 2497-2498 -LRB- denotes (
T6456 2498-2504 IN denotes unless
T6457 2505-2514 RB denotes otherwise
T6458 2515-2521 VBN denotes stated
T6459 2521-2522 -RRB- denotes )
T6460 2522-2523 , denotes ,
T6461 2524-2533 JJ denotes cell-free
T6462 2534-2539 NNS denotes media
T6463 2540-2544 VBD denotes were
T6464 2545-2554 VBN denotes collected
T6465 2555-2558 CC denotes and
T6466 2559-2565 VBN denotes stored
T6467 2566-2568 IN denotes at
T6468 2569-2572 CD denotes -20
T6469 2572-2573 CD denotes °
T6470 2573-2574 NNP denotes C
T6471 2575-2580 IN denotes until
T6472 2581-2588 VBN denotes assayed
T6473 2588-2589 . denotes .
T6474 2590-2593 DT denotes All
T6475 2594-2602 NNS denotes cultures
T6476 2603-2607 VBD denotes were
T6477 2608-2611 VBN denotes set
T6478 2612-2614 RP denotes up
T6479 2615-2617 IN denotes in
T6480 2618-2628 NN denotes triplicate
T6481 2628-2629 , denotes ,
T6482 2630-2633 CC denotes and
T6483 2634-2641 NNS denotes results
T6484 2642-2645 VBP denotes are
T6485 2646-2655 VBN denotes expressed
T6486 2656-2658 IN denotes as
T6487 2659-2664 NNS denotes means
T6488 2665-2666 VBP denotes ±
T6489 2667-2670 NNP denotes SEM
T6490 2670-2671 . denotes .
T6978 2673-2676 CD denotes CD4
T6979 2676-2677 CD denotes +
T6980 2678-2684 NNP denotes T-cell
T6981 2685-2694 NN denotes isolation
T6982 2695-2697 IN denotes by
T6983 2698-2702 NNP denotes MACS
T6984 2703-2711 JJ denotes Anti-CD4
T6985 2712-2722 NNS denotes microbeads
T6986 2723-2727 VBD denotes were
T6987 2728-2732 VBN denotes used
T6988 2733-2744 RB denotes essentially
T6989 2745-2747 IN denotes as
T6990 2748-2759 VBN denotes recommended
T6991 2760-2762 IN denotes by
T6992 2763-2766 DT denotes the
T6993 2767-2779 NN denotes manufacturer
T6994 2780-2781 -LRB- denotes (
T6995 2781-2789 NNP denotes Miltenyi
T6996 2789-2790 -RRB- denotes )
T6997 2791-2792 NNP denotes [
T6998 2792-2794 CD denotes 19
T6999 2794-2795 NNP denotes ]
T7000 2795-2796 . denotes .
T7001 2797-2801 NNP denotes PBMC
T7002 2802-2806 VBD denotes were
T7003 2807-2818 VBN denotes resuspended
T7004 2819-2821 IN denotes in
T7005 2822-2824 CD denotes 80
T7006 2825-2827 NN denotes μl
T7007 2828-2830 IN denotes of
T7008 2831-2834 NNP denotes FBS
T7009 2835-2843 VBG denotes staining
T7010 2844-2850 NN denotes buffer
T7011 2850-2851 . denotes .
T7012 2852-2860 JJ denotes Anti-CD4
T7013 2861-2871 NNS denotes microbeads
T7014 2872-2873 -LRB- denotes (
T7015 2873-2875 CD denotes 20
T7016 2876-2878 NN denotes μl
T7017 2878-2879 -RRB- denotes )
T7018 2880-2884 VBD denotes were
T7019 2885-2890 VBN denotes added
T7020 2891-2894 CC denotes and
T7021 2895-2904 VBN denotes incubated
T7022 2905-2908 IN denotes for
T7023 2909-2911 CD denotes 15
T7024 2912-2915 NN denotes min
T7025 2916-2918 IN denotes at
T7026 2919-2920 CD denotes 6
T7027 2921-2923 CD denotes 12
T7028 2923-2924 NN denotes °
T7029 2924-2926 NNP denotes C.
T7030 2927-2937 NNP denotes Saturating
T7031 2938-2945 NNS denotes amounts
T7032 2946-2948 IN denotes of
T7033 2949-2972 JJ denotes fluorochrome-conjugated
T7034 2973-2983 NNS denotes antibodies
T7035 2984-2988 VBD denotes were
T7036 2989-2994 VBN denotes added
T7037 2995-2998 IN denotes for
T7038 2999-3000 DT denotes a
T7039 3001-3008 JJ denotes further
T7040 3009-3011 CD denotes 10
T7041 3012-3015 NN denotes min
T7042 3015-3016 . denotes .
T7043 3017-3022 NNS denotes Cells
T7044 3023-3027 VBD denotes were
T7045 3028-3035 VBN denotes diluted
T7046 3036-3038 IN denotes in
T7047 3039-3042 CD denotes 2.5
T7048 3043-3045 NN denotes ml
T7049 3046-3048 IN denotes of
T7050 3049-3052 NNP denotes FBS
T7051 3053-3061 VBG denotes staining
T7052 3062-3068 NN denotes buffer
T7053 3068-3069 , denotes ,
T7054 3070-3078 VBN denotes pelleted
T7055 3078-3079 , denotes ,
T7056 3080-3091 VBN denotes resuspended
T7057 3092-3094 IN denotes in
T7058 3095-3098 CD denotes 500
T7059 3099-3101 NN denotes μl
T7060 3102-3105 CC denotes and
T7061 3106-3118 RB denotes magnetically
T7062 3119-3128 VBD denotes separated
T7063 3128-3129 , denotes ,
T7064 3130-3137 RB denotes usually
T7065 3138-3140 IN denotes on
T7066 3141-3143 DT denotes an
T7067 3144-3152 NNP denotes AutoMACS
T7068 3153-3159 NN denotes magnet
T7069 3160-3166 VBN denotes fitted
T7070 3167-3171 IN denotes with
T7071 3172-3173 DT denotes a
T7072 3174-3178 NNP denotes MACS
T7073 3179-3181 NNP denotes MS
T7074 3182-3188 NN denotes column
T7075 3188-3189 . denotes .
T7076 3190-3202 JJ denotes Flow-through
T7077 3203-3206 CC denotes and
T7078 3207-3210 CD denotes two
T7079 3211-3212 CD denotes 1
T7080 3213-3215 NN denotes ml
T7081 3216-3222 NNS denotes washes
T7082 3223-3227 VBD denotes were
T7083 3228-3237 VBN denotes collected
T7084 3238-3240 IN denotes as
T7085 3241-3244 DT denotes the
T7086 3245-3253 JJ denotes negative
T7087 3254-3262 NN denotes fraction
T7088 3262-3263 . denotes .
T7089 3264-3272 JJ denotes Enriched
T7090 3273-3278 NNS denotes cells
T7091 3279-3283 VBD denotes were
T7092 3284-3293 VBN denotes collected
T7093 3294-3296 IN denotes in
T7094 3297-3300 CD denotes two
T7095 3301-3304 CD denotes 0.5
T7096 3305-3307 NN denotes ml
T7097 3308-3316 NNS denotes aliquots
T7098 3317-3321 IN denotes from
T7099 3322-3325 DT denotes the
T7100 3326-3332 NN denotes column
T7101 3333-3338 IN denotes after
T7102 3339-3346 NN denotes removal
T7103 3347-3351 IN denotes from
T7104 3352-3355 DT denotes the
T7105 3356-3362 NN denotes magnet
T7106 3362-3363 . denotes .
T7107 3364-3377 RB denotes Alternatively
T7108 3377-3378 , denotes ,
T7109 3379-3384 NNS denotes cells
T7110 3385-3392 VBD denotes stained
T7111 3393-3397 IN denotes with
T7112 3398-3406 JJ denotes anti-CD4
T7113 3407-3420 NN denotes phycoerythrin
T7114 3421-3425 VBD denotes were
T7115 3426-3432 VBN denotes washed
T7116 3432-3433 , denotes ,
T7117 3434-3446 RB denotes magnetically
T7118 3447-3454 VBN denotes labeled
T7119 3455-3459 IN denotes with
T7120 3460-3478 JJ denotes anti-phycoerythrin
T7121 3479-3489 NNS denotes microbeads
T7122 3490-3491 -LRB- denotes (
T7123 3491-3493 CD denotes 20
T7124 3494-3496 NN denotes μl
T7125 3497-3502 VBD denotes added
T7126 3503-3505 TO denotes to
T7127 3506-3508 CD denotes 80
T7128 3509-3511 NN denotes μl
T7129 3512-3514 IN denotes of
T7130 3515-3519 NN denotes cell
T7131 3520-3530 NN denotes suspension
T7132 3530-3531 : denotes ;
T7133 3532-3534 CD denotes 15
T7134 3535-3538 NN denotes min
T7135 3538-3539 , denotes ,
T7136 3540-3541 CD denotes 6
T7137 3542-3544 CD denotes 12
T7138 3544-3545 NN denotes °
T7139 3545-3546 NNP denotes C
T7140 3546-3547 -RRB- denotes )
T7141 3547-3548 , denotes ,
T7142 3549-3552 CC denotes and
T7143 3553-3565 RB denotes magnetically
T7144 3566-3575 VBD denotes separated
T7145 3576-3578 IN denotes as
T7146 3579-3588 VBN denotes described
T7147 3589-3594 IN denotes above
T7148 3594-3595 . denotes .
T7149 3596-3599 DT denotes The
T7150 3600-3606 NN denotes purity
T7151 3607-3609 IN denotes of
T7152 3610-3615 NNS denotes cells
T7153 3616-3619 VBD denotes was
T7154 3620-3628 VBN denotes assessed
T7155 3629-3631 IN denotes by
T7156 3632-3636 NN denotes flow
T7157 3637-3647 JJ denotes cytometric
T7158 3648-3656 NN denotes analysis
T7159 3657-3659 IN denotes of
T7160 3660-3667 JJ denotes stained
T7161 3668-3673 NNS denotes cells
T7162 3674-3676 IN denotes on
T7163 3677-3678 DT denotes a
T7164 3679-3683 NNP denotes FACS
T7165 3684-3691 NNP denotes Vantage
T7166 3692-3698 NN denotes sorter
T7167 3698-3699 . denotes .
T7168 3700-3704 JJS denotes Most
T7169 3705-3706 -LRB- denotes (
T7170 3706-3710 JJR denotes more
T7171 3711-3715 IN denotes than
T7172 3716-3718 CD denotes 97
T7173 3718-3719 NN denotes %
T7174 3719-3720 -RRB- denotes )
T7175 3721-3723 IN denotes of
T7176 3724-3727 DT denotes the
T7177 3728-3736 JJ denotes isolated
T7178 3737-3742 NNS denotes cells
T7179 3743-3746 VBD denotes had
T7180 3747-3750 DT denotes the
T7181 3751-3754 NNP denotes CD4
T7182 3755-3756 NNP denotes T
T7183 3757-3761 NN denotes cell
T7184 3762-3768 NN denotes marker
T7185 3768-3769 . denotes .
T7843 3771-3784 JJ denotes Enzyme-linked
T7844 3785-3798 JJ denotes immunosorbent
T7845 3799-3804 NN denotes assay
T7846 3805-3807 IN denotes of
T7847 3808-3813 NNP denotes IL-17
T7848 3814-3819 NNP denotes IL-17
T7849 3820-3822 IN denotes in
T7850 3823-3830 NN denotes culture
T7851 3831-3843 NNS denotes supernatants
T7852 3844-3847 VBD denotes was
T7853 3848-3856 VBN denotes measured
T7854 3857-3859 IN denotes by
T7855 3860-3868 NN denotes sandwich
T7856 3869-3882 JJ denotes enzyme-linked
T7857 3883-3896 JJ denotes immunosorbent
T7858 3897-3902 NN denotes assay
T7859 3903-3905 IN denotes as
T7860 3906-3915 VBN denotes described
T7861 3916-3926 RB denotes previously
T7862 3927-3928 NNP denotes [
T7863 3928-3930 CD denotes 20
T7864 3930-3931 NNP denotes ]
T7865 3931-3932 . denotes .
T7866 3933-3935 IN denotes In
T7867 3936-3941 NN denotes brief
T7868 3941-3942 , denotes ,
T7869 3943-3944 DT denotes a
T7870 3945-3952 JJ denotes 96-well
T7871 3953-3958 NN denotes plate
T7872 3959-3960 -LRB- denotes (
T7873 3960-3964 NNP denotes Nunc
T7874 3964-3965 -RRB- denotes )
T7875 3966-3969 VBD denotes was
T7876 3970-3976 VBN denotes coated
T7877 3977-3981 IN denotes with
T7878 3982-3983 CD denotes 4
T7879 3984-3986 NN denotes μg
T7880 3986-3987 NN denotes /
T7881 3987-3989 NN denotes ml
T7882 3990-4000 JJ denotes monoclonal
T7883 4001-4011 NNS denotes antibodies
T7884 4012-4019 IN denotes against
T7885 4020-4025 NNP denotes IL-17
T7886 4026-4027 -LRB- denotes (
T7887 4027-4028 NNP denotes R
T7888 4029-4030 CC denotes &
T7889 4031-4032 NNP denotes D
T7890 4033-4040 NNPS denotes Systems
T7891 4040-4041 -RRB- denotes )
T7892 4042-4044 IN denotes at
T7893 4045-4046 CD denotes 4
T7894 4046-4047 CD denotes °
T7895 4047-4048 NNP denotes C
T7896 4049-4058 JJ denotes overnight
T7897 4058-4059 . denotes .
T7898 4060-4065 IN denotes After
T7899 4066-4074 VBG denotes blocking
T7900 4075-4079 IN denotes with
T7901 4080-4098 JJ denotes phosphate-buffered
T7902 4099-4107 CD denotes saline/1
T7903 4107-4108 NN denotes %
T7904 4109-4115 JJ denotes bovine
T7905 4116-4121 NN denotes serum
T7906 4122-4129 NN denotes albumin
T7907 4130-4131 -LRB- denotes (
T7908 4131-4134 NNP denotes BSA
T7909 4134-4135 -RRB- denotes )
T7910 4135-4136 VBP denotes /
T7911 4136-4140 CD denotes 0.05
T7912 4140-4141 NN denotes %
T7913 4142-4147 CD denotes Tween
T7914 4148-4150 CD denotes 20
T7915 4151-4154 IN denotes for
T7916 4155-4156 CD denotes 2
T7917 4157-4162 NNS denotes hours
T7918 4163-4165 IN denotes at
T7919 4166-4170 NN denotes room
T7920 4171-4182 NN denotes temperature
T7921 4183-4184 -LRB- denotes (
T7922 4184-4186 CD denotes 22
T7923 4187-4189 CD denotes 25
T7924 4189-4190 NN denotes °
T7925 4190-4191 NNP denotes C
T7926 4191-4192 -RRB- denotes )
T7927 4192-4193 , denotes ,
T7928 4194-4198 NN denotes test
T7929 4199-4206 NNS denotes samples
T7930 4207-4210 CC denotes and
T7931 4211-4214 DT denotes the
T7932 4215-4223 JJ denotes standard
T7933 4224-4235 JJ denotes recombinant
T7934 4236-4241 NNP denotes IL-17
T7935 4242-4243 -LRB- denotes (
T7936 4243-4244 NNP denotes R
T7937 4245-4246 CC denotes &
T7938 4247-4248 NNP denotes D
T7939 4249-4256 NNPS denotes Systems
T7940 4256-4257 -RRB- denotes )
T7941 4258-4262 VBD denotes were
T7942 4263-4268 VBN denotes added
T7943 4269-4271 TO denotes to
T7944 4272-4275 DT denotes the
T7945 4276-4283 JJ denotes 96-well
T7946 4284-4289 NN denotes plate
T7947 4290-4293 CC denotes and
T7948 4294-4303 VBD denotes incubated
T7949 4304-4306 IN denotes at
T7950 4307-4311 NN denotes room
T7951 4312-4323 NN denotes temperature
T7952 4324-4327 IN denotes for
T7953 4328-4329 CD denotes 2
T7954 4330-4335 NNS denotes hours
T7955 4335-4336 . denotes .
T7956 4337-4343 NNS denotes Plates
T7957 4344-4348 VBD denotes were
T7958 4349-4355 VBN denotes washed
T7959 4356-4360 CD denotes four
T7960 4361-4366 NNS denotes times
T7961 4367-4371 IN denotes with
T7962 4372-4390 JJ denotes phosphate-buffered
T7963 4391-4403 NNP denotes saline/Tween
T7964 4404-4406 CD denotes 20
T7965 4406-4407 , denotes ,
T7966 4408-4411 CC denotes and
T7967 4412-4416 RB denotes then
T7968 4417-4426 VBN denotes incubated
T7969 4427-4431 IN denotes with
T7970 4432-4435 CD denotes 500
T7971 4436-4441 JJ denotes ng/ml
T7972 4442-4454 VBN denotes biotinylated
T7973 4455-4460 NN denotes mouse
T7974 4461-4471 NN denotes monoclonal
T7975 4472-4482 NNS denotes antibodies
T7976 4483-4490 IN denotes against
T7977 4491-4496 NNP denotes IL-17
T7978 4497-4498 -LRB- denotes (
T7979 4498-4499 NNP denotes R
T7980 4500-4501 CC denotes &
T7981 4502-4503 NNP denotes D
T7982 4504-4511 NNPS denotes Systems
T7983 4511-4512 -RRB- denotes )
T7984 4513-4516 IN denotes for
T7985 4517-4518 CD denotes 2
T7986 4519-4524 NNS denotes hours
T7987 4525-4527 IN denotes at
T7988 4528-4532 NN denotes room
T7989 4533-4544 NN denotes temperature
T7990 4544-4545 . denotes .
T7991 4546-4551 IN denotes After
T7992 4552-4559 VBG denotes washing
T7993 4559-4560 , denotes ,
T7994 4561-4573 NNP denotes streptavidin
T7995 4574-4582 JJ denotes alkaline
T7996 4583-4592 NN denotes phosphate
T7997 4593-4604 JJ denotes horseradish
T7998 4605-4615 JJ denotes peroxidase
T7999 4616-4625 NN denotes conjugate
T8000 4626-4627 -LRB- denotes (
T8001 4627-4632 NNP denotes Sigma
T8002 4632-4633 -RRB- denotes )
T8003 4634-4637 VBD denotes was
T8004 4638-4647 VBN denotes incubated
T8005 4648-4651 IN denotes for
T8006 4652-4653 CD denotes 2
T8007 4654-4659 NNS denotes hours
T8008 4659-4660 , denotes ,
T8009 4661-4665 RB denotes then
T8010 4666-4672 VBD denotes washed
T8011 4673-4678 RB denotes again
T8012 4679-4682 CC denotes and
T8013 4683-4692 VBN denotes incubated
T8014 4693-4697 IN denotes with
T8015 4698-4699 CD denotes 1
T8016 4700-4705 NN denotes mg/ml
T8017 4706-4719 NN denotes p-nitrophenyl
T8018 4720-4729 NN denotes phosphate
T8019 4730-4731 -LRB- denotes (
T8020 4731-4736 NNP denotes Sigma
T8021 4736-4737 -RRB- denotes )
T8022 4738-4747 VBN denotes dissolved
T8023 4748-4750 IN denotes in
T8024 4751-4765 NN denotes diethanolamine
T8025 4766-4767 -LRB- denotes (
T8026 4767-4772 NNP denotes Sigma
T8027 4772-4773 -RRB- denotes )
T8028 4774-4776 TO denotes to
T8029 4777-4784 VB denotes develop
T8030 4785-4788 DT denotes the
T8031 4789-4794 NN denotes color
T8032 4795-4803 NN denotes reaction
T8033 4803-4804 . denotes .
T8034 4805-4808 DT denotes The
T8035 4809-4817 NN denotes reaction
T8036 4818-4821 VBD denotes was
T8037 4822-4829 VBN denotes stopped
T8038 4830-4832 IN denotes by
T8039 4833-4836 DT denotes the
T8040 4837-4845 NN denotes addition
T8041 4846-4848 IN denotes of
T8042 4849-4850 CD denotes 1
T8043 4851-4852 NNP denotes M
T8044 4853-4857 NNP denotes NaOH
T8045 4858-4861 CC denotes and
T8046 4862-4865 DT denotes the
T8047 4866-4873 JJ denotes optical
T8048 4874-4881 NN denotes density
T8049 4882-4884 IN denotes of
T8050 4885-4889 DT denotes each
T8051 4890-4894 NN denotes well
T8052 4895-4898 VBD denotes was
T8053 4899-4903 VBN denotes read
T8054 4904-4906 IN denotes at
T8055 4907-4910 CD denotes 405
T8056 4911-4913 NN denotes nm
T8057 4913-4914 . denotes .
T8058 4915-4918 DT denotes The
T8059 4919-4924 JJR denotes lower
T8060 4925-4930 NN denotes limit
T8061 4931-4933 IN denotes of
T8062 4934-4939 NNP denotes IL-17
T8063 4940-4949 NN denotes detection
T8064 4950-4953 VBD denotes was
T8065 4954-4956 CD denotes 10
T8066 4957-4962 NN denotes pg/ml
T8067 4962-4963 . denotes .
T8068 4964-4975 JJ denotes Recombinant
T8069 4976-4981 JJ denotes human
T8070 4982-4987 NN denotes IL-17
T8071 4988-4995 VBN denotes diluted
T8072 4996-4998 IN denotes in
T8073 4999-5006 NN denotes culture
T8074 5007-5013 NN denotes medium
T8075 5014-5017 VBD denotes was
T8076 5018-5022 VBN denotes used
T8077 5023-5025 IN denotes as
T8078 5026-5027 DT denotes a
T8079 5028-5039 NN denotes calibration
T8080 5040-5048 NN denotes standard
T8081 5048-5049 , denotes ,
T8082 5050-5057 VBG denotes ranging
T8083 5058-5062 IN denotes from
T8084 5063-5065 CD denotes 10
T8085 5066-5068 TO denotes to
T8086 5069-5073 CD denotes 2000
T8087 5074-5079 NN denotes pg/ml
T8088 5079-5080 . denotes .
T8089 5081-5082 DT denotes A
T8090 5083-5091 JJ denotes standard
T8091 5092-5097 NN denotes curve
T8092 5098-5101 VBD denotes was
T8093 5102-5107 VBN denotes drawn
T8094 5108-5110 IN denotes by
T8095 5111-5119 VBG denotes plotting
T8096 5120-5127 JJ denotes optical
T8097 5128-5135 NN denotes density
T8098 5136-5143 IN denotes against
T8099 5144-5147 DT denotes the
T8100 5148-5151 VB denotes log
T8101 5152-5154 IN denotes of
T8102 5155-5158 DT denotes the
T8103 5159-5172 NN denotes concentration
T8104 5173-5175 IN denotes of
T8105 5176-5187 JJ denotes recombinant
T8106 5188-5197 NNS denotes cytokines
T8107 5197-5198 , denotes ,
T8108 5199-5202 CC denotes and
T8109 5203-5207 VBN denotes used
T8110 5208-5211 IN denotes for
T8111 5212-5225 NN denotes determination
T8112 5226-5228 IN denotes of
T8113 5229-5234 NNP denotes IL-17
T8114 5235-5237 IN denotes in
T8115 5238-5242 NN denotes test
T8116 5243-5250 NNS denotes samples
T8117 5250-5251 . denotes .
T8766 5253-5267 NN denotes Quantification
T8767 5268-5270 IN denotes of
T8768 5271-5276 NNP denotes IL-17
T8769 5277-5281 NNP denotes mRNA
T8770 5282-5284 IN denotes by
T8771 5285-5301 JJ denotes semiquantitative
T8772 5302-5309 NN denotes reverse
T8773 5310-5323 NN denotes transcription
T8774 5324-5334 NN denotes polymerase
T8775 5335-5340 NN denotes chain
T8776 5341-5349 NN denotes reaction
T8777 5350-5354 NNP denotes PBMC
T8778 5355-5359 VBD denotes were
T8779 5360-5369 VBN denotes incubated
T8780 5370-5374 IN denotes with
T8781 5375-5382 JJ denotes various
T8782 5383-5397 NNS denotes concentrations
T8783 5398-5400 IN denotes of
T8784 5401-5409 JJ denotes anti-CD3
T8785 5410-5412 IN denotes in
T8786 5413-5416 DT denotes the
T8787 5417-5425 NN denotes presence
T8788 5426-5428 CC denotes or
T8789 5429-5436 NN denotes absence
T8790 5437-5439 IN denotes of
T8791 5440-5450 NNS denotes inhibitors
T8792 5451-5452 -LRB- denotes (
T8793 5452-5460 CD denotes LY294002
T8794 5460-5461 , denotes ,
T8795 5462-5466 NNP denotes PDTC
T8796 5466-5467 -RRB- denotes )
T8797 5467-5468 . denotes .
T8798 5469-5474 IN denotes After
T8799 5475-5477 CD denotes 16
T8800 5478-5483 NNS denotes hours
T8801 5484-5486 IN denotes of
T8802 5487-5497 NN denotes incubation
T8803 5497-5498 , denotes ,
T8804 5499-5503 NNP denotes mRNA
T8805 5504-5507 VBD denotes was
T8806 5508-5517 VBN denotes extracted
T8807 5518-5522 IN denotes with
T8808 5523-5529 NNP denotes RNAzol
T8809 5530-5531 NNP denotes B
T8810 5532-5533 -LRB- denotes (
T8811 5533-5539 NNP denotes Biotex
T8812 5540-5552 NNPS denotes Laboratories
T8813 5552-5553 , denotes ,
T8814 5554-5561 NNP denotes Houston
T8815 5561-5562 , denotes ,
T8816 5563-5565 NNP denotes TX
T8817 5565-5566 , denotes ,
T8818 5567-5570 NNP denotes USA
T8819 5570-5571 -RRB- denotes )
T8820 5572-5574 IN denotes in
T8821 5575-5585 NN denotes accordance
T8822 5586-5590 IN denotes with
T8823 5591-5594 DT denotes the
T8824 5595-5607 NN denotes manufacturer
T8825 5607-5609 POS denotes 's
T8826 5610-5622 NNS denotes instructions
T8827 5622-5623 . denotes .
T8828 5624-5631 VB denotes Reverse
T8829 5632-5645 NN denotes transcription
T8830 5646-5648 IN denotes of
T8831 5649-5650 CD denotes 2
T8832 5651-5653 NN denotes μg
T8833 5654-5656 IN denotes of
T8834 5657-5662 JJ denotes total
T8835 5663-5667 NNP denotes mRNA
T8836 5668-5671 VBD denotes was
T8837 5672-5681 VBN denotes performed
T8838 5682-5684 IN denotes at
T8839 5685-5687 CD denotes 42
T8840 5687-5688 CD denotes °
T8841 5688-5689 NNP denotes C
T8842 5690-5695 VBG denotes using
T8843 5696-5699 DT denotes the
T8844 5700-5711 NNP denotes Superscript
T8845 5711-5712 NN denotes ™
T8846 5713-5720 NN denotes reverse
T8847 5721-5734 NN denotes transcription
T8848 5735-5741 NN denotes system
T8849 5742-5743 -LRB- denotes (
T8850 5743-5749 NNP denotes Takara
T8851 5749-5750 , denotes ,
T8852 5751-5756 NNP denotes Shiga
T8853 5756-5757 , denotes ,
T8854 5758-5763 NNP denotes Japan
T8855 5763-5764 -RRB- denotes )
T8856 5764-5765 . denotes .
T8857 5766-5769 NNP denotes PCR
T8858 5770-5783 NN denotes amplification
T8859 5784-5786 IN denotes of
T8860 5787-5791 NNP denotes cDNA
T8861 5792-5800 NNS denotes aliquots
T8862 5801-5804 VBD denotes was
T8863 5805-5814 VBN denotes performed
T8864 5815-5817 IN denotes by
T8865 5818-5824 VBG denotes adding
T8866 5825-5828 CD denotes 2.5
T8867 5829-5831 NNP denotes mM
T8868 5832-5837 NNP denotes dNTPs
T8869 5837-5838 , denotes ,
T8870 5839-5842 CD denotes 2.5
T8871 5843-5844 NNP denotes U
T8872 5845-5847 IN denotes of
T8873 5848-5851 NNP denotes Taq
T8874 5852-5855 NNP denotes DNA
T8875 5856-5866 NN denotes polymerase
T8876 5867-5868 -LRB- denotes (
T8877 5868-5874 NNP denotes Takara
T8878 5874-5875 -RRB- denotes )
T8879 5876-5879 CC denotes and
T8880 5880-5884 CD denotes 0.25
T8881 5885-5887 NNP denotes μM
T8882 5888-5890 IN denotes of
T8883 5891-5896 NN denotes sense
T8884 5897-5900 CC denotes and
T8885 5901-5910 JJ denotes antisense
T8886 5911-5918 NNS denotes primers
T8887 5918-5919 . denotes .
T8888 5920-5923 DT denotes The
T8889 5924-5932 NN denotes reaction
T8890 5933-5936 VBD denotes was
T8891 5937-5946 VBN denotes performed
T8892 5947-5949 IN denotes in
T8893 5950-5953 NNP denotes PCR
T8894 5954-5960 NN denotes buffer
T8895 5961-5962 -LRB- denotes (
T8896 5962-5965 CD denotes 1.5
T8897 5966-5968 NNP denotes mM
T8898 5969-5974 NNP denotes MgCl2
T8899 5974-5975 , denotes ,
T8900 5976-5978 CD denotes 50
T8901 5979-5981 NNP denotes mM
T8902 5982-5985 NNP denotes KCl
T8903 5985-5986 , denotes ,
T8904 5987-5989 CD denotes 10
T8905 5990-5992 NNP denotes mM
T8906 5993-6001 NNP denotes Tris-HCl
T8907 6001-6002 , denotes ,
T8908 6003-6005 NNP denotes pH
T8909 6006-6009 CD denotes 8.3
T8910 6009-6010 -RRB- denotes )
T8911 6011-6013 IN denotes in
T8912 6014-6015 DT denotes a
T8913 6016-6021 JJ denotes total
T8914 6022-6028 NN denotes volume
T8915 6029-6031 IN denotes of
T8916 6032-6034 CD denotes 25
T8917 6035-6037 NN denotes μl
T8918 6037-6038 . denotes .
T8919 6039-6042 DT denotes The
T8920 6043-6052 JJ denotes following
T8921 6053-6058 NN denotes sense
T8922 6059-6062 CC denotes and
T8923 6063-6072 JJ denotes antisense
T8924 6073-6080 NNS denotes primers
T8925 6081-6084 IN denotes for
T8926 6085-6089 DT denotes each
T8927 6090-6099 NNS denotes molecules
T8928 6100-6104 VBD denotes were
T8929 6105-6109 VBN denotes used
T8930 6109-6110 : denotes :
T8931 6111-6116 JJ denotes IL-17
T8932 6117-6122 NN denotes sense
T8933 6122-6123 , denotes ,
T8934 6124-6125 CD denotes 5
T8935 6125-6126 '' denotes '
T8936 6126-6127 : denotes -
T8937 6127-6130 NNP denotes ATG
T8938 6131-6134 NNP denotes ACT
T8939 6135-6138 NNP denotes CCT
T8940 6139-6142 NNP denotes GGG
T8941 6143-6146 NNP denotes AAG
T8942 6147-6150 NNP denotes ACC
T8943 6151-6154 NNP denotes TCA
T8944 6155-6160 NN denotes TTG-3
T8945 6160-6161 '' denotes '
T8946 6161-6162 : denotes ;
T8947 6163-6168 NNP denotes IL-17
T8948 6169-6178 NN denotes antisense
T8949 6178-6179 , denotes ,
T8950 6180-6181 CD denotes 5
T8951 6181-6182 '' denotes '
T8952 6182-6183 : denotes -
T8953 6183-6186 NNP denotes TTA
T8954 6187-6190 NNP denotes GGC
T8955 6191-6194 NNP denotes CAC
T8956 6195-6198 NNP denotes ATG
T8957 6199-6202 NNP denotes GTG
T8958 6203-6206 NNP denotes GAC
T8959 6207-6210 NNP denotes AAT
T8960 6211-6216 NN denotes CGG-3
T8961 6216-6217 '' denotes '
T8962 6217-6218 : denotes ;
T8963 6219-6245 JJ denotes glyceraldehyde-3-phosphate
T8964 6246-6259 NN denotes dehydrogenase
T8965 6260-6261 -LRB- denotes (
T8966 6261-6266 NNP denotes GAPDH
T8967 6266-6267 -RRB- denotes )
T8968 6268-6273 NN denotes sense
T8969 6273-6274 , denotes ,
T8970 6275-6276 CD denotes 5
T8971 6276-6277 '' denotes '
T8972 6277-6278 : denotes -
T8973 6278-6281 NNP denotes CGA
T8974 6282-6285 NNP denotes TGC
T8975 6286-6289 NNP denotes TGG
T8976 6290-6293 NNP denotes GCG
T8977 6294-6297 NNP denotes TGA
T8978 6298-6301 NNP denotes GTA
T8979 6302-6305 NN denotes C-3
T8980 6305-6306 '' denotes '
T8981 6306-6307 : denotes ;
T8982 6308-6313 NNP denotes GAPDH
T8983 6314-6323 NN denotes antisense
T8984 6323-6324 , denotes ,
T8985 6325-6326 CD denotes 5
T8986 6326-6327 '' denotes '
T8987 6327-6328 : denotes -
T8988 6328-6331 NNP denotes CGT
T8989 6332-6335 NNP denotes TCA
T8990 6336-6339 NNP denotes GCT
T8991 6340-6343 NNP denotes CAG
T8992 6344-6347 NNP denotes GGA
T8993 6348-6351 NNP denotes TGA
T8994 6352-6356 NN denotes CC-3
T8995 6356-6357 '' denotes '
T8996 6357-6358 . denotes .
T8997 6359-6368 NNS denotes Reactions
T8998 6369-6373 VBD denotes were
T8999 6374-6383 VBN denotes processed
T9000 6384-6386 IN denotes in
T9001 6387-6388 DT denotes a
T9002 6389-6392 NNP denotes DNA
T9003 6393-6400 JJ denotes thermal
T9004 6401-6407 NN denotes cycler
T9005 6408-6409 -LRB- denotes (
T9006 6409-6421 NNP denotes Perkin-Elmer
T9007 6422-6427 NNP denotes Cetus
T9008 6427-6428 , denotes ,
T9009 6429-6436 NNP denotes Norwalk
T9010 6436-6437 , denotes ,
T9011 6438-6440 NNP denotes CT
T9012 6440-6441 , denotes ,
T9013 6442-6445 NNP denotes USA
T9014 6445-6446 -RRB- denotes )
T9015 6447-6454 IN denotes through
T9016 6455-6461 NNS denotes cycles
T9017 6462-6465 IN denotes for
T9018 6466-6468 CD denotes 30
T9019 6469-6470 PRP denotes s
T9020 6471-6473 IN denotes of
T9021 6474-6486 NN denotes denaturation
T9022 6487-6489 IN denotes at
T9023 6490-6492 CD denotes 94
T9024 6492-6493 CD denotes °
T9025 6493-6494 NNP denotes C
T9026 6494-6495 , denotes ,
T9027 6496-6497 CD denotes 1
T9028 6498-6501 NN denotes min
T9029 6502-6504 IN denotes of
T9030 6505-6514 VBG denotes annealing
T9031 6515-6517 IN denotes at
T9032 6518-6520 CD denotes 56
T9033 6520-6521 CD denotes °
T9034 6521-6522 NNP denotes C
T9035 6523-6526 IN denotes for
T9036 6527-6532 NNP denotes GAPDH
T9037 6533-6536 CC denotes and
T9038 6537-6542 NNP denotes IL-17
T9039 6542-6543 , denotes ,
T9040 6544-6552 VBN denotes followed
T9041 6553-6555 IN denotes by
T9042 6556-6557 CD denotes 1
T9043 6558-6561 NN denotes min
T9044 6562-6564 IN denotes of
T9045 6565-6575 NN denotes elongation
T9046 6576-6578 IN denotes at
T9047 6579-6581 CD denotes 72
T9048 6581-6582 CD denotes °
T9049 6582-6584 NNP denotes C.
T9050 6585-6588 NNP denotes PCR
T9051 6589-6595 NNS denotes rounds
T9052 6596-6600 VBD denotes were
T9053 6601-6609 VBN denotes repeated
T9054 6610-6613 IN denotes for
T9055 6614-6616 CD denotes 25
T9056 6617-6623 NNS denotes cycles
T9057 6624-6628 DT denotes each
T9058 6629-6632 IN denotes for
T9059 6633-6637 DT denotes both
T9060 6638-6643 NNP denotes GAPDH
T9061 6644-6647 CC denotes and
T9062 6648-6653 NNP denotes IL-17
T9063 6653-6654 : denotes ;
T9064 6655-6659 DT denotes this
T9065 6660-6663 VBD denotes was
T9066 6664-6674 VBN denotes determined
T9067 6675-6677 IN denotes as
T9068 6678-6685 VBG denotes falling
T9069 6686-6692 IN denotes within
T9070 6693-6696 DT denotes the
T9071 6697-6708 JJ denotes exponential
T9072 6709-6714 NN denotes phase
T9073 6715-6717 IN denotes of
T9074 6718-6731 NN denotes amplification
T9075 6732-6735 IN denotes for
T9076 6736-6740 DT denotes each
T9077 6741-6749 NN denotes molecule
T9078 6749-6750 . denotes .
T9079 6751-6754 DT denotes The
T9080 6755-6760 NN denotes level
T9081 6761-6763 IN denotes of
T9082 6764-6768 NNP denotes mRNA
T9083 6769-6779 NN denotes expression
T9084 6780-6783 VBD denotes was
T9085 6784-6793 VBN denotes presented
T9086 6794-6796 IN denotes as
T9087 6797-6798 DT denotes a
T9088 6799-6804 NN denotes ratio
T9089 6805-6807 IN denotes of
T9090 6808-6813 NNP denotes IL-17
T9091 6814-6817 NNP denotes PCR
T9092 6818-6825 NN denotes product
T9093 6826-6830 IN denotes over
T9094 6831-6836 NNP denotes GAPDH
T9095 6837-6844 NN denotes product
T9096 6844-6845 . denotes .
T9686 6847-6854 JJ denotes Western
T9687 6855-6859 NN denotes blot
T9688 6860-6868 NN denotes analysis
T9689 6869-6871 IN denotes of
T9690 6872-6875 NNP denotes Akt
T9691 6875-6876 , denotes ,
T9692 6877-6891 VBD denotes phosphorylated
T9693 6892-6895 NNP denotes Akt
T9694 6896-6899 CC denotes and
T9695 6900-6905 NNP denotes IκB-α
T9696 6906-6910 NNP denotes PBMC
T9697 6911-6915 VBD denotes were
T9698 6916-6925 VBN denotes incubated
T9699 6926-6930 IN denotes with
T9700 6931-6939 JJ denotes anti-CD3
T9701 6940-6941 -LRB- denotes (
T9702 6941-6943 CD denotes 10
T9703 6944-6946 NN denotes μg
T9704 6946-6947 NN denotes /
T9705 6947-6949 NN denotes ml
T9706 6949-6950 -RRB- denotes )
T9707 6951-6953 IN denotes in
T9708 6954-6957 DT denotes the
T9709 6958-6966 NN denotes presence
T9710 6967-6969 CC denotes or
T9711 6970-6977 NN denotes absence
T9712 6978-6980 IN denotes of
T9713 6981-6989 NNP denotes LY294002
T9714 6990-6991 -LRB- denotes (
T9715 6991-6993 CD denotes 20
T9716 6994-6996 NNP denotes μM
T9717 6996-6997 -RRB- denotes )
T9718 6997-6998 . denotes .
T9719 6999-7004 IN denotes After
T9720 7005-7015 NN denotes incubation
T9721 7016-7019 IN denotes for
T9722 7020-7021 CD denotes 1
T9723 7022-7026 NN denotes hour
T9724 7026-7027 , denotes ,
T9725 7028-7033 JJ denotes whole
T9726 7034-7038 NN denotes cell
T9727 7039-7046 NNS denotes lysates
T9728 7047-7051 VBD denotes were
T9729 7052-7060 VBN denotes prepared
T9730 7061-7065 IN denotes from
T9731 7066-7071 IN denotes about
T9732 7072-7075 CD denotes 107
T9733 7076-7081 NNS denotes cells
T9734 7082-7084 IN denotes by
T9735 7085-7099 NN denotes homogenization
T9736 7100-7102 IN denotes in
T9737 7103-7106 DT denotes the
T9738 7107-7112 NN denotes lysis
T9739 7113-7119 NN denotes buffer
T9740 7119-7120 , denotes ,
T9741 7121-7124 CC denotes and
T9742 7125-7136 VBD denotes centrifuged
T9743 7137-7139 IN denotes at
T9744 7140-7146 CD denotes 14,000
T9745 7147-7153 NN denotes r.p.m.
T9746 7154-7155 -LRB- denotes (
T9747 7155-7161 CD denotes 19,000
T9748 7162-7163 NN denotes g
T9749 7163-7164 -RRB- denotes )
T9750 7165-7168 IN denotes for
T9751 7169-7171 CD denotes 15
T9752 7172-7175 NN denotes min
T9753 7175-7176 . denotes .
T9754 7177-7184 NN denotes Protein
T9755 7185-7199 NNS denotes concentrations
T9756 7200-7202 IN denotes in
T9757 7203-7206 DT denotes the
T9758 7207-7219 NNS denotes supernatants
T9759 7220-7224 VBD denotes were
T9760 7225-7235 VBN denotes determined
T9761 7236-7240 IN denotes with
T9762 7241-7244 DT denotes the
T9763 7245-7253 NNP denotes Bradford
T9764 7254-7260 NN denotes method
T9765 7261-7262 -LRB- denotes (
T9766 7262-7269 NNP denotes Bio-Rad
T9767 7269-7270 , denotes ,
T9768 7271-7279 NNP denotes Hercules
T9769 7279-7280 , denotes ,
T9770 7281-7283 NNP denotes CA
T9771 7283-7284 , denotes ,
T9772 7285-7288 NNP denotes USA
T9773 7288-7289 -RRB- denotes )
T9774 7289-7290 . denotes .
T9775 7291-7298 NN denotes Protein
T9776 7299-7306 NNS denotes samples
T9777 7307-7311 VBD denotes were
T9778 7312-7321 VBN denotes separated
T9779 7322-7324 IN denotes by
T9780 7325-7327 CD denotes 10
T9781 7327-7328 NN denotes %
T9782 7329-7332 NNP denotes SDS
T9783 7333-7337 NNP denotes PAGE
T9784 7338-7341 CC denotes and
T9785 7342-7353 VBN denotes transferred
T9786 7354-7356 TO denotes to
T9787 7357-7358 DT denotes a
T9788 7359-7373 JJ denotes nitrocellulose
T9789 7374-7382 NN denotes membrane
T9790 7383-7384 -LRB- denotes (
T9791 7384-7392 NNP denotes Amersham
T9792 7393-7402 NNP denotes Pharmacia
T9793 7403-7410 NNP denotes Biotech
T9794 7410-7411 , denotes ,
T9795 7412-7419 NNP denotes Uppsala
T9796 7419-7420 , denotes ,
T9797 7421-7427 NNP denotes Sweden
T9798 7427-7428 -RRB- denotes )
T9799 7428-7429 . denotes .
T9800 7430-7433 IN denotes For
T9801 7434-7441 JJ denotes western
T9802 7442-7455 NN denotes hybridization
T9803 7455-7456 , denotes ,
T9804 7457-7465 NN denotes membrane
T9805 7466-7469 VBD denotes was
T9806 7470-7482 VBN denotes preincubated
T9807 7483-7487 IN denotes with
T9808 7488-7491 CD denotes 0.1
T9809 7491-7492 NN denotes %
T9810 7493-7500 VBD denotes skimmed
T9811 7501-7505 NN denotes milk
T9812 7506-7508 IN denotes in
T9813 7509-7514 NNP denotes TBS-T
T9814 7515-7521 NN denotes buffer
T9815 7522-7523 -LRB- denotes (
T9816 7523-7526 CD denotes 0.1
T9817 7526-7527 NN denotes %
T9818 7528-7533 CD denotes Tween
T9819 7534-7536 CD denotes 20
T9820 7537-7539 IN denotes in
T9821 7540-7553 JJ denotes Tris-buffered
T9822 7554-7560 NN denotes saline
T9823 7560-7561 -RRB- denotes )
T9824 7562-7564 IN denotes at
T9825 7565-7569 NN denotes room
T9826 7570-7581 NN denotes temperature
T9827 7582-7585 IN denotes for
T9828 7586-7587 CD denotes 2
T9829 7588-7593 NNS denotes hours
T9830 7593-7594 , denotes ,
T9831 7595-7599 RB denotes then
T9832 7600-7607 JJ denotes primary
T9833 7608-7618 NNS denotes antibodies
T9834 7619-7626 IN denotes against
T9835 7627-7630 NNP denotes Akt
T9836 7630-7631 , denotes ,
T9837 7632-7646 VBD denotes phosphorylated
T9838 7647-7650 NNP denotes Akt
T9839 7651-7654 CC denotes and
T9840 7655-7660 NNP denotes IκB-α
T9841 7661-7662 -LRB- denotes (
T9842 7662-7666 NNP denotes Cell
T9843 7667-7676 NNP denotes Signaling
T9844 7677-7687 NNP denotes Technology
T9845 7688-7692 NNP denotes Inc.
T9846 7692-7693 , denotes ,
T9847 7694-7701 NNP denotes Beverly
T9848 7701-7702 , denotes ,
T9849 7703-7705 NNP denotes MA
T9850 7705-7706 , denotes ,
T9851 7707-7710 NNP denotes USA
T9852 7710-7711 -RRB- denotes )
T9853 7711-7712 , denotes ,
T9854 7713-7720 VBN denotes diluted
T9855 7721-7727 CD denotes 1:1000
T9856 7728-7730 IN denotes in
T9857 7731-7732 CD denotes 5
T9858 7732-7733 NN denotes %
T9859 7734-7743 NN denotes BSA/TBS-T
T9860 7743-7744 , denotes ,
T9861 7745-7749 VBD denotes were
T9862 7750-7755 VBN denotes added
T9863 7756-7759 CC denotes and
T9864 7760-7769 VBN denotes incubated
T9865 7770-7779 JJ denotes overnight
T9866 7780-7782 IN denotes at
T9867 7783-7784 CD denotes 4
T9868 7784-7785 CD denotes °
T9869 7785-7786 NNP denotes C
T9870 7786-7787 . denotes .
T9871 7788-7793 IN denotes After
T9872 7794-7801 VBG denotes washing
T9873 7802-7806 CD denotes four
T9874 7807-7812 NNS denotes times
T9875 7813-7817 IN denotes with
T9876 7818-7823 NNP denotes TBS-T
T9877 7823-7824 , denotes ,
T9878 7825-7836 JJ denotes horseradish
T9879 7837-7858 JJ denotes peroxidase-conjugated
T9880 7859-7868 JJ denotes secondary
T9881 7869-7879 NNS denotes antibodies
T9882 7880-7884 VBD denotes were
T9883 7885-7890 VBN denotes added
T9884 7891-7894 CC denotes and
T9885 7895-7902 VBN denotes allowed
T9886 7903-7905 TO denotes to
T9887 7906-7914 VB denotes incubate
T9888 7915-7918 IN denotes for
T9889 7919-7920 CD denotes 1
T9890 7921-7925 NN denotes hour
T9891 7926-7928 IN denotes at
T9892 7929-7933 NN denotes room
T9893 7934-7945 NN denotes temperature
T9894 7945-7946 . denotes .
T9895 7947-7952 IN denotes After
T9896 7953-7958 NNP denotes TBS-T
T9897 7959-7966 VBG denotes washing
T9898 7966-7967 , denotes ,
T9899 7968-7978 VBN denotes hybridized
T9900 7979-7984 NNS denotes bands
T9901 7985-7989 VBD denotes were
T9902 7990-7998 VBN denotes detected
T9903 7999-8003 IN denotes with
T9904 8004-8007 DT denotes the
T9905 8008-8016 JJ denotes enhanced
T9906 8017-8034 NN denotes chemiluminescence
T9907 8035-8036 -LRB- denotes (
T9908 8036-8039 NNP denotes ECL
T9909 8039-8040 -RRB- denotes )
T9910 8041-8050 NN denotes detection
T9911 8051-8054 NN denotes kit
T9912 8055-8058 CC denotes and
T9913 8059-8072 JJ denotes Hyperfilm-ECL
T9914 8073-8081 NNS denotes reagents
T9915 8082-8083 -LRB- denotes (
T9916 8083-8091 NNP denotes Amersham
T9917 8092-8101 NNP denotes Pharmacia
T9918 8101-8102 -RRB- denotes )
T9919 8102-8103 . denotes .
T10457 8105-8108 NNP denotes Gel
T10458 8109-8123 JJ denotes mobility-shift
T10459 8124-8129 NN denotes assay
T10460 8130-8132 IN denotes of
T10461 8133-8138 JJ denotes NF-κB
T10462 8139-8146 JJ denotes binding
T10463 8147-8151 NN denotes site
T10464 8152-8159 NNP denotes Nuclear
T10465 8160-8168 NNS denotes proteins
T10466 8169-8173 VBD denotes were
T10467 8174-8183 VBN denotes extracted
T10468 8184-8188 IN denotes from
T10469 8189-8194 IN denotes about
T10470 8195-8196 CD denotes 5
T10471 8197-8198 CD denotes ×
T10472 8199-8202 CD denotes 106
T10473 8203-8207 NNP denotes PBMC
T10474 8207-8208 . denotes .
T10475 8209-8224 NNP denotes Oligonucleotide
T10476 8225-8231 NNS denotes probes
T10477 8232-8244 VBG denotes encompassing
T10478 8245-8248 DT denotes the
T10479 8249-8254 JJ denotes NF-κB
T10480 8255-8262 JJ denotes binding
T10481 8263-8267 NN denotes site
T10482 8268-8270 IN denotes of
T10483 8271-8274 DT denotes the
T10484 8275-8280 JJ denotes human
T10485 8281-8286 NNP denotes IL-17
T10486 8287-8295 NN denotes promoter
T10487 8296-8297 -LRB- denotes (
T10488 8297-8298 CD denotes 5
T10489 8298-8299 '' denotes '
T10490 8299-8300 : denotes -
T10491 8300-8303 NNP denotes ATG
T10492 8304-8307 NNP denotes ACC
T10493 8308-8311 NNP denotes TGG
T10494 8312-8315 NNP denotes AAA
T10495 8316-8319 NNP denotes TAC
T10496 8320-8323 NNP denotes CCA
T10497 8324-8327 NNP denotes AAA
T10498 8328-8333 NN denotes TTC-3
T10499 8333-8334 '' denotes '
T10500 8334-8335 -RRB- denotes )
T10501 8336-8340 VBD denotes were
T10502 8341-8350 VBN denotes generated
T10503 8351-8353 IN denotes by
T10504 8354-8355 CD denotes 5
T10505 8355-8356 '' denotes '
T10506 8356-8357 : denotes -
T10507 8357-8360 NN denotes end
T10508 8361-8369 VBG denotes labeling
T10509 8370-8372 IN denotes of
T10510 8373-8376 DT denotes the
T10511 8377-8382 NN denotes sense
T10512 8383-8389 NN denotes strand
T10513 8390-8394 IN denotes with
T10514 8395-8396 NNP denotes [
T10515 8396-8401 NNP denotes γ-32P
T10516 8401-8402 NNP denotes ]
T10517 8402-8406 NNP denotes dATP
T10518 8407-8408 -LRB- denotes (
T10519 8408-8416 NNP denotes Amersham
T10520 8417-8426 NNP denotes Pharmacia
T10521 8426-8427 -RRB- denotes )
T10522 8428-8431 CC denotes and
T10523 8432-8434 CD denotes T4
T10524 8435-8449 NN denotes polynucleotide
T10525 8450-8456 NN denotes kinase
T10526 8457-8458 -LRB- denotes (
T10527 8458-8464 NNP denotes TaKaRa
T10528 8464-8465 -RRB- denotes )
T10529 8465-8466 . denotes .
T10530 8467-8481 NNP denotes Unincorporated
T10531 8482-8493 NNS denotes nucleotides
T10532 8494-8498 VBD denotes were
T10533 8499-8506 VBN denotes removed
T10534 8507-8509 IN denotes by
T10535 8510-8517 NNP denotes NucTrap
T10536 8518-8523 NN denotes probe
T10537 8524-8536 NN denotes purification
T10538 8537-8544 NNS denotes columns
T10539 8545-8546 -LRB- denotes (
T10540 8546-8556 NNP denotes Stratagene
T10541 8556-8557 , denotes ,
T10542 8558-8560 NNP denotes La
T10543 8561-8566 NNP denotes Jolla
T10544 8566-8567 , denotes ,
T10545 8568-8570 NNP denotes CA
T10546 8570-8571 , denotes ,
T10547 8572-8575 NNP denotes USA
T10548 8575-8576 -RRB- denotes )
T10549 8576-8577 . denotes .
T10550 8578-8585 NNP denotes Nuclear
T10551 8586-8594 NNS denotes extracts
T10552 8595-8596 -LRB- denotes (
T10553 8596-8597 CD denotes 2
T10554 8598-8600 NN denotes μg
T10555 8601-8603 IN denotes of
T10556 8604-8611 NN denotes protein
T10557 8611-8612 -RRB- denotes )
T10558 8613-8617 VBD denotes were
T10559 8618-8627 VBN denotes incubated
T10560 8628-8632 IN denotes with
T10561 8633-8645 JJ denotes radiolabeled
T10562 8646-8649 NNP denotes DNA
T10563 8650-8656 NNS denotes probes
T10564 8657-8658 -LRB- denotes (
T10565 8658-8660 CD denotes 10
T10566 8661-8663 NN denotes ng
T10567 8663-8664 : denotes ;
T10568 8665-8672 CD denotes 100,000
T10569 8673-8679 NN denotes c.p.m.
T10570 8679-8680 -RRB- denotes )
T10571 8681-8684 IN denotes for
T10572 8685-8687 CD denotes 30
T10573 8688-8691 NN denotes min
T10574 8692-8694 IN denotes at
T10575 8695-8699 NN denotes room
T10576 8700-8711 NN denotes temperature
T10577 8712-8714 IN denotes in
T10578 8715-8717 CD denotes 20
T10579 8718-8720 NN denotes μl
T10580 8721-8723 IN denotes of
T10581 8724-8731 JJ denotes binding
T10582 8732-8738 NN denotes buffer
T10583 8739-8749 VBG denotes consisting
T10584 8750-8752 IN denotes of
T10585 8753-8755 CD denotes 20
T10586 8756-8758 NNP denotes mM
T10587 8759-8767 NNP denotes Tris-HCl
T10588 8767-8768 , denotes ,
T10589 8769-8771 NNP denotes pH
T10590 8772-8775 CD denotes 7.9
T10591 8775-8776 , denotes ,
T10592 8777-8779 CD denotes 50
T10593 8780-8782 NNP denotes mM
T10594 8783-8786 NNP denotes KCl
T10595 8786-8787 , denotes ,
T10596 8788-8789 CD denotes 1
T10597 8790-8792 NNP denotes mM
T10598 8793-8807 NN denotes dithiothreitol
T10599 8807-8808 , denotes ,
T10600 8809-8812 CD denotes 0.5
T10601 8813-8815 NNP denotes mM
T10602 8816-8820 NNP denotes EDTA
T10603 8820-8821 , denotes ,
T10604 8822-8823 CD denotes 5
T10605 8823-8824 NN denotes %
T10606 8825-8833 NN denotes glycerol
T10607 8833-8834 , denotes ,
T10608 8835-8836 CD denotes 1
T10609 8837-8842 NN denotes mg/ml
T10610 8843-8846 NNP denotes BSA
T10611 8846-8847 , denotes ,
T10612 8848-8851 CD denotes 0.2
T10613 8851-8852 NN denotes %
T10614 8853-8860 NNP denotes Nonidet
T10615 8861-8864 NNP denotes P40
T10616 8865-8868 CC denotes and
T10617 8869-8871 CD denotes 50
T10618 8872-8874 NN denotes ng
T10619 8874-8875 NN denotes /
T10620 8875-8877 NN denotes μl
T10621 8878-8882 NN denotes poly
T10622 8882-8883 -LRB- denotes (
T10623 8883-8888 JJ denotes dI-dC
T10624 8888-8889 -RRB- denotes )
T10625 8889-8890 . denotes .
T10626 8891-8898 NNS denotes Samples
T10627 8899-8903 VBD denotes were
T10628 8904-8913 VBN denotes subjected
T10629 8914-8916 TO denotes to
T10630 8917-8932 NNS denotes electrophoresis
T10631 8933-8935 IN denotes on
T10632 8936-8949 VBG denotes nondenaturing
T10633 8950-8951 CD denotes 5
T10634 8951-8952 NN denotes %
T10635 8953-8967 NN denotes polyacrylamide
T10636 8968-8972 NNS denotes gels
T10637 8973-8975 IN denotes in
T10638 8976-8979 CD denotes 0.5
T10639 8980-8981 CD denotes ×
T10640 8982-8998 NNP denotes Tris-borate-EDTA
T10641 8999-9005 NN denotes buffer
T10642 9006-9007 -LRB- denotes (
T10643 9007-9009 NNP denotes pH
T10644 9010-9013 CD denotes 8.0
T10645 9013-9014 -RRB- denotes )
T10646 9015-9017 IN denotes at
T10647 9018-9021 CD denotes 100
T10648 9022-9024 NNP denotes V.
T10649 9025-9029 NNP denotes Gels
T10650 9030-9034 VBD denotes were
T10651 9035-9040 VBN denotes dried
T10652 9041-9046 IN denotes under
T10653 9047-9053 NN denotes vacuum
T10654 9054-9057 CC denotes and
T10655 9058-9065 VBN denotes exposed
T10656 9066-9068 TO denotes to
T10657 9069-9074 NNP denotes Kodak
T10658 9075-9081 NNP denotes X-OMAT
T10659 9082-9086 NN denotes film
T10660 9087-9089 IN denotes at
T10661 9090-9093 CD denotes -70
T10662 9093-9094 CD denotes °
T10663 9094-9095 NNP denotes C
T10664 9096-9100 IN denotes with
T10665 9101-9113 VBG denotes intensifying
T10666 9114-9121 NNS denotes screens
T10667 9121-9122 . denotes .
T10668 9123-9129 NN denotes Rabbit
T10669 9130-9140 NN denotes polyclonal
T10670 9141-9151 NNS denotes antibodies
T10671 9152-9159 IN denotes against
T10672 9160-9165 NNP denotes NF-κB
T10673 9166-9174 VBZ denotes subunits
T10674 9175-9178 CD denotes p50
T10675 9178-9179 , denotes ,
T10676 9180-9183 CD denotes p65
T10677 9184-9187 CC denotes and
T10678 9188-9193 NNP denotes c-Rel
T10679 9194-9198 VBD denotes were
T10680 9199-9203 IN denotes from
T10681 9204-9209 NNP denotes Santa
T10682 9210-9214 NNP denotes Cruz
T10683 9215-9228 NNP denotes Biotechnology
T10684 9229-9230 -LRB- denotes (
T10685 9230-9235 NNP denotes Santa
T10686 9236-9240 NNP denotes Cruz
T10687 9240-9241 , denotes ,
T10688 9242-9244 NNP denotes CA
T10689 9244-9245 , denotes ,
T10690 9246-9249 NNP denotes USA
T10691 9249-9250 -RRB- denotes )
T10692 9250-9251 . denotes .
T10901 9253-9257 NNP denotes Cell
T10902 9258-9267 NN denotes viability
T10903 9268-9269 -LRB- denotes (
T10904 9269-9275 NNP denotes Trypan
T10905 9276-9280 JJ denotes blue
T10906 9281-9284 NN denotes dye
T10907 9285-9294 NN denotes exclusion
T10908 9295-9300 NN denotes assay
T10909 9300-9301 -RRB- denotes )
T10910 9302-9305 IN denotes For
T10911 9306-9310 NN denotes cell
T10912 9311-9320 NN denotes viability
T10913 9321-9327 NNS denotes assays
T10914 9327-9328 , denotes ,
T10915 9329-9332 DT denotes the
T10916 9333-9339 NN denotes trypan
T10917 9340-9344 JJ denotes blue
T10918 9345-9348 NN denotes dye
T10919 9349-9358 NN denotes exclusion
T10920 9359-9365 NN denotes method
T10921 9366-9369 VBD denotes was
T10922 9370-9374 VBN denotes used
T10923 9375-9377 TO denotes to
T10924 9378-9386 VB denotes evaluate
T10925 9387-9390 DT denotes the
T10926 9391-9400 NN denotes potential
T10927 9401-9403 IN denotes of
T10928 9404-9410 JJ denotes direct
T10929 9411-9420 JJ denotes cytotoxic
T10930 9421-9427 NN denotes effect
T10931 9428-9430 IN denotes of
T10932 9431-9441 NNS denotes inhibitors
T10933 9442-9444 IN denotes on
T10934 9445-9450 NNS denotes cells
T10935 9450-9451 . denotes .
T10936 9452-9457 IN denotes After
T10937 9458-9468 NN denotes incubation
T10938 9469-9472 IN denotes for
T10939 9473-9475 CD denotes 24
T10940 9476-9481 NNS denotes hours
T10941 9481-9482 , denotes ,
T10942 9483-9486 DT denotes the
T10943 9487-9492 NNS denotes cells
T10944 9493-9497 VBD denotes were
T10945 9498-9507 VBN denotes harvested
T10946 9508-9511 CC denotes and
T10947 9512-9515 DT denotes the
T10948 9516-9526 NN denotes percentage
T10949 9527-9531 NN denotes cell
T10950 9532-9541 NN denotes viability
T10951 9542-9545 VBD denotes was
T10952 9546-9556 VBN denotes calculated
T10953 9557-9561 IN denotes with
T10954 9562-9565 DT denotes the
T10955 9566-9573 NN denotes formula
T10956 9574-9577 CD denotes 100
T10957 9578-9579 NN denotes ×
T10958 9580-9581 -LRB- denotes (
T10959 9581-9587 NN denotes number
T10960 9588-9590 IN denotes of
T10961 9591-9597 JJ denotes viable
T10962 9598-9610 NN denotes cells/number
T10963 9611-9613 IN denotes of
T10964 9614-9618 DT denotes both
T10965 9619-9625 JJ denotes viable
T10966 9626-9629 CC denotes and
T10967 9630-9634 JJ denotes dead
T10968 9635-9640 NNS denotes cells
T10969 9640-9641 -RRB- denotes )
T10970 9642-9643 NNP denotes [
T10971 9643-9645 CD denotes 21
T10972 9645-9646 NNP denotes ]
T10973 9646-9647 . denotes .
T11046 9649-9660 NNP denotes Statistical
T11047 9661-9669 NN denotes analysis
T11048 9670-9674 NNS denotes Data
T11049 9675-9678 VBP denotes are
T11050 9679-9688 VBN denotes expressed
T11051 9689-9691 IN denotes as
T11052 9692-9697 NNS denotes means
T11053 9698-9699 VBP denotes ±
T11054 9700-9703 NNP denotes SEM
T11055 9703-9704 . denotes .
T11056 9705-9716 NNP denotes Statistical
T11057 9717-9725 NN denotes analysis
T11058 9726-9729 VBD denotes was
T11059 9730-9739 VBN denotes performed
T11060 9740-9744 IN denotes with
T11061 9745-9752 NN denotes Student
T11062 9752-9754 POS denotes 's
T11063 9755-9761 JJS denotes t-test
T11064 9762-9765 IN denotes for
T11065 9766-9773 VBN denotes matched
T11066 9774-9779 NNS denotes pairs
T11067 9779-9780 . denotes .
T11068 9781-9782 NNP denotes P
T11069 9783-9789 VBZ denotes values
T11070 9790-9794 JJR denotes less
T11071 9795-9799 IN denotes than
T11072 9800-9804 CD denotes 0.05
T11073 9805-9809 VBD denotes were
T11074 9810-9820 VBN denotes considered
T11075 9821-9832 JJ denotes significant
T11076 9832-9833 . denotes .
R2640 T5038 T5042 nsubjpass Patients,obtained
R2641 T5039 T5040 amod Informed,consent
R2642 T5040 T5042 nsubjpass consent,obtained
R2643 T5041 T5042 auxpass was,obtained
R2644 T5042 T5042 ROOT obtained,obtained
R2645 T5043 T5042 prep from,obtained
R2646 T5044 T5045 nummod 24,patients
R2647 T5045 T5043 pobj patients,from
R2648 T5046 T5045 punct (,patients
R2649 T5047 T5048 nummod 5,men
R2650 T5048 T5045 appos men,patients
R2651 T5049 T5048 cc and,men
R2652 T5050 T5051 nummod 19,women
R2653 T5051 T5048 conj women,men
R2654 T5052 T5045 punct ),patients
R2655 T5053 T5042 prep with,obtained
R2656 T5054 T5053 pobj RA,with
R2657 T5055 T5056 nsubj who,fulfilled
R2658 T5056 T5042 conj fulfilled,obtained
R2659 T5057 T5060 det the,criteria
R2660 T5058 T5060 nummod 1987,criteria
R2661 T5059 T5060 amod revised,criteria
R2662 T5060 T5056 dobj criteria,fulfilled
R2663 T5061 T5060 prep of,criteria
R2664 T5062 T5064 det the,College
R2665 T5063 T5064 compound American,College
R2666 T5064 T5061 pobj College,of
R2667 T5065 T5064 prep of,College
R2668 T5066 T5065 pobj Rheumatology,of
R2669 T5067 T5064 punct (,College
R2670 T5068 T5072 advmod formerly,Association
R2671 T5069 T5072 det the,Association
R2672 T5070 T5072 compound American,Association
R2673 T5071 T5072 compound Rheumatism,Association
R2674 T5072 T5064 appos Association,College
R2675 T5073 T5064 punct ),College
R2676 T5074 T5076 nmod [,]
R2677 T5075 T5076 nummod 17,]
R2678 T5076 T5060 appos ],criteria
R2679 T5077 T5042 punct .,obtained
R2680 T5078 T5079 det The,age
R2681 T5079 T5085 nsubj age,was
R2682 T5080 T5079 prep of,age
R2683 T5081 T5082 det the,patients
R2684 T5082 T5080 pobj patients,of
R2685 T5083 T5082 prep with,patients
R2686 T5084 T5083 pobj RA,with
R2687 T5085 T5085 ROOT was,was
R2688 T5086 T5087 nummod 50,±
R2689 T5087 T5085 attr ±,was
R2690 T5088 T5087 nummod 8,±
R2691 T5089 T5092 punct (,SEM
R2692 T5090 T5092 amod mean,SEM
R2693 T5091 T5092 compound ±,SEM
R2694 T5092 T5087 appos SEM,±
R2695 T5093 T5092 punct ),SEM
R2696 T5094 T5087 appos years,±
R2697 T5095 T5096 punct (,range
R2698 T5096 T5087 appos range,±
R2699 T5097 T5096 nummod 23,range
R2700 T5098 T5099 nummod 71,years
R2701 T5099 T5096 appos years,range
R2702 T5100 T5096 punct ),range
R2703 T5101 T5087 punct .,±
R2704 T5102 T5103 det All,medications
R2705 T5103 T5105 nsubjpass medications,stopped
R2706 T5104 T5105 auxpass were,stopped
R2707 T5105 T5105 ROOT stopped,stopped
R2708 T5106 T5107 nummod 48,hours
R2709 T5107 T5108 npadvmod hours,before
R2710 T5108 T5105 prep before,stopped
R2711 T5109 T5108 pobj entry,before
R2712 T5110 T5109 prep to,entry
R2713 T5111 T5112 det the,study
R2714 T5112 T5110 pobj study,to
R2715 T5113 T5105 punct .,stopped
R2716 T5114 T5116 nsubjpass Comparisons,made
R2717 T5115 T5116 auxpass were,made
R2718 T5116 T5116 ROOT made,made
R2719 T5117 T5116 prep with,made
R2720 T5118 T5119 nummod 14,patients
R2721 T5119 T5117 pobj patients,with
R2722 T5120 T5119 prep with,patients
R2723 T5121 T5120 pobj OA,with
R2724 T5122 T5121 punct (,OA
R2725 T5123 T5124 nummod 3,men
R2726 T5124 T5121 appos men,OA
R2727 T5125 T5124 cc and,men
R2728 T5126 T5127 nummod 11,women
R2729 T5127 T5124 conj women,men
R2730 T5128 T5121 punct ),OA
R2731 T5129 T5120 cc and,with
R2732 T5130 T5120 conj with,with
R2733 T5131 T5133 nummod 14,controls
R2734 T5132 T5133 amod healthy,controls
R2735 T5133 T5130 pobj controls,with
R2736 T5134 T5133 punct (,controls
R2737 T5135 T5136 nummod 3,men
R2738 T5136 T5133 appos men,controls
R2739 T5137 T5136 cc and,men
R2740 T5138 T5139 nummod 11,women
R2741 T5139 T5136 conj women,men
R2742 T5140 T5133 punct ),controls
R2743 T5141 T5142 nsubj who,had
R2744 T5142 T5133 relcl had,controls
R2745 T5143 T5145 det no,diseases
R2746 T5144 T5145 amod rheumatic,diseases
R2747 T5145 T5142 dobj diseases,had
R2748 T5146 T5116 punct .,made
R2749 T5147 T5149 det The,ages
R2750 T5148 T5149 amod mean,ages
R2751 T5149 T5159 nsubj ages,were
R2752 T5150 T5149 prep of,ages
R2753 T5151 T5152 det the,patients
R2754 T5152 T5150 pobj patients,of
R2755 T5153 T5152 prep with,patients
R2756 T5154 T5153 pobj OA,with
R2757 T5155 T5154 cc and,OA
R2758 T5156 T5158 det the,controls
R2759 T5157 T5158 amod healthy,controls
R2760 T5158 T5154 conj controls,OA
R2761 T5159 T5159 ROOT were,were
R2762 T5160 T5161 nummod 50,±
R2763 T5161 T5159 attr ±,were
R2764 T5162 T5163 nummod 8,years
R2765 T5163 T5161 appos years,±
R2766 T5164 T5165 punct (,range
R2767 T5165 T5163 parataxis range,years
R2768 T5166 T5165 nummod 34,range
R2769 T5167 T5168 nummod 68,years
R2770 T5168 T5165 appos years,range
R2771 T5169 T5165 punct ),range
R2772 T5170 T5163 cc and,years
R2773 T5171 T5172 nummod 30,±
R2774 T5172 T5163 conj ±,years
R2775 T5173 T5174 nummod 6,years
R2776 T5174 T5172 appos years,±
R2777 T5175 T5176 punct (,range
R2778 T5176 T5174 parataxis range,years
R2779 T5177 T5176 nummod 24,range
R2780 T5178 T5179 nummod 57,years
R2781 T5179 T5176 appos years,range
R2782 T5180 T5176 punct ),range
R2783 T5181 T5159 punct .,were
R2784 T5182 T5183 amod Informed,consent
R2785 T5183 T5185 nsubjpass consent,obtained
R2786 T5184 T5185 auxpass was,obtained
R2787 T5185 T5185 ROOT obtained,obtained
R2788 T5186 T5185 punct ",",obtained
R2789 T5187 T5185 cc and,obtained
R2790 T5188 T5189 det the,protocol
R2791 T5189 T5191 nsubjpass protocol,approved
R2792 T5190 T5191 auxpass was,approved
R2793 T5191 T5185 conj approved,obtained
R2794 T5192 T5191 agent by,approved
R2795 T5193 T5195 det the,University
R2796 T5194 T5195 compound Catholic,University
R2797 T5195 T5201 nmod University,Committee
R2798 T5196 T5195 prep of,University
R2799 T5197 T5196 pobj Korea,of
R2800 T5198 T5200 compound Human,Ethics
R2801 T5199 T5200 compound Research,Ethics
R2802 T5200 T5201 compound Ethics,Committee
R2803 T5201 T5192 pobj Committee,by
R2804 T5202 T5191 punct .,approved
R2971 T5665 T5667 compound Reagents,IL-17
R2972 T5666 T5667 compound Recombinant,IL-17
R2973 T5667 T5695 nsubjpass IL-17,purchased
R2974 T5668 T5667 punct ",",IL-17
R2975 T5669 T5667 conj IL-18,IL-17
R2976 T5670 T5669 punct ",",IL-18
R2977 T5671 T5669 conj IL-15,IL-18
R2978 T5672 T5671 punct ",",IL-15
R2979 T5673 T5675 nmod monocyte,protein-1
R2980 T5674 T5675 compound chemoattractant,protein-1
R2981 T5675 T5671 appos protein-1,IL-15
R2982 T5676 T5677 punct (,MCP-1
R2983 T5677 T5675 appos MCP-1,protein-1
R2984 T5678 T5677 punct ),MCP-1
R2985 T5679 T5671 punct ",",IL-15
R2986 T5680 T5682 nmod macrophage,protein
R2987 T5681 T5682 amod inflammatory,protein
R2988 T5682 T5667 appos protein,IL-17
R2989 T5683 T5684 punct (,MIP
R2990 T5684 T5682 appos MIP,protein
R2991 T5685 T5684 punct ),MIP
R2992 T5686 T5687 nummod -1,α
R2993 T5687 T5667 appos α,IL-17
R2994 T5688 T5687 punct ",",α
R2995 T5689 T5687 conj MIP-1β,α
R2996 T5690 T5689 punct ",",MIP-1β
R2997 T5691 T5689 conj IL-6,MIP-1β
R2998 T5692 T5691 cc and,IL-6
R2999 T5693 T5691 conj IL-8,IL-6
R3000 T5694 T5695 auxpass were,purchased
R3001 T5695 T5695 ROOT purchased,purchased
R3002 T5696 T5695 prep from,purchased
R3003 T5697 T5700 nmod R,systems
R3004 T5698 T5697 cc &,R
R3005 T5699 T5697 conj D,R
R3006 T5700 T5696 pobj systems,from
R3007 T5701 T5700 punct (,systems
R3008 T5702 T5700 appos Minneapolis,systems
R3009 T5703 T5702 punct ",",Minneapolis
R3010 T5704 T5702 conj MN,Minneapolis
R3011 T5705 T5704 punct ",",MN
R3012 T5706 T5704 npadvmod USA,MN
R3013 T5707 T5704 punct ),MN
R3014 T5708 T5695 punct .,purchased
R3015 T5709 T5712 amod Recombinant,factor
R3016 T5710 T5712 amod transforming,factor
R3017 T5711 T5712 compound growth,factor
R3018 T5712 T5719 nsubjpass factor,purchased
R3019 T5713 T5714 punct (,TGF
R3020 T5714 T5712 appos TGF,factor
R3021 T5715 T5714 punct ),TGF
R3022 T5716 T5717 punct -,β
R3023 T5717 T5712 appos β,factor
R3024 T5718 T5719 auxpass was,purchased
R3025 T5719 T5719 ROOT purchased,purchased
R3026 T5720 T5719 prep from,purchased
R3027 T5721 T5720 pobj Peprotech,from
R3028 T5722 T5721 punct (,Peprotech
R3029 T5723 T5721 appos London,Peprotech
R3030 T5724 T5723 punct ",",London
R3031 T5725 T5723 appos UK,London
R3032 T5726 T5723 punct ),London
R3033 T5727 T5719 punct .,purchased
R3034 T5728 T5729 amod Recombinant,TNF-α
R3035 T5729 T5733 nsubjpass TNF-α,purchased
R3036 T5730 T5729 cc and,TNF-α
R3037 T5731 T5729 conj IL-1,TNF-α
R3038 T5732 T5733 auxpass were,purchased
R3039 T5733 T5733 ROOT purchased,purchased
R3040 T5734 T5733 prep from,purchased
R3041 T5735 T5736 compound Endogen,Inc.
R3042 T5736 T5734 pobj Inc.,from
R3043 T5737 T5736 punct (,Inc.
R3044 T5738 T5736 npadvmod Cambridge,Inc.
R3045 T5739 T5738 punct ",",Cambridge
R3046 T5740 T5738 conj MA,Cambridge
R3047 T5741 T5740 punct ",",MA
R3048 T5742 T5740 conj USA,MA
R3049 T5743 T5740 punct ),MA
R3050 T5744 T5733 punct .,purchased
R3051 T5745 T5746 compound Cyclosporin,A
R3052 T5746 T5748 nsubjpass A,provided
R3053 T5747 T5748 auxpass was,provided
R3054 T5748 T5748 ROOT provided,provided
R3055 T5749 T5748 agent by,provided
R3056 T5750 T5751 compound Sandos,Ltd.
R3057 T5751 T5749 pobj Ltd.,by
R3058 T5752 T5751 punct (,Ltd.
R3059 T5753 T5751 npadvmod Basel,Ltd.
R3060 T5754 T5753 punct ",",Basel
R3061 T5755 T5753 appos Switzerland,Basel
R3062 T5756 T5753 punct ),Basel
R3063 T5757 T5748 punct .,provided
R3064 T5758 T5776 nsubjpass Phytohemagglutinin,obtained
R3065 T5759 T5760 punct (,PHA
R3066 T5760 T5758 appos PHA,Phytohemagglutinin
R3067 T5761 T5760 punct ),PHA
R3068 T5762 T5758 punct ",",Phytohemagglutinin
R3069 T5763 T5764 amod pyrrolidine,dithiocarbamate
R3070 T5764 T5758 appos dithiocarbamate,Phytohemagglutinin
R3071 T5765 T5766 punct (,PDTC
R3072 T5766 T5764 appos PDTC,dithiocarbamate
R3073 T5767 T5766 punct ),PDTC
R3074 T5768 T5764 punct ",",dithiocarbamate
R3075 T5769 T5764 conj rapamycin,dithiocarbamate
R3076 T5770 T5769 punct ",",rapamycin
R3077 T5771 T5769 conj dexamethasone,rapamycin
R3078 T5772 T5771 cc and,dexamethasone
R3079 T5773 T5771 conj curcumin,dexamethasone
R3080 T5774 T5776 auxpass were,obtained
R3081 T5775 T5776 dep all,obtained
R3082 T5776 T5776 ROOT obtained,obtained
R3083 T5777 T5776 prep from,obtained
R3084 T5778 T5781 det the,Co.
R3085 T5779 T5781 compound Sigma,Co.
R3086 T5780 T5781 compound Chemical,Co.
R3087 T5781 T5777 pobj Co.,from
R3088 T5782 T5781 punct (,Co.
R3089 T5783 T5784 compound St,Louis
R3090 T5784 T5781 appos Louis,Co.
R3091 T5785 T5781 punct ",",Co.
R3092 T5786 T5781 conj MA,Co.
R3093 T5787 T5786 punct ",",MA
R3094 T5788 T5786 conj USA,MA
R3095 T5789 T5776 punct ),obtained
R3096 T5790 T5776 punct .,obtained
R3097 T5791 T5793 amod Anti-CD3,antibody
R3098 T5792 T5793 amod monoclonal,antibody
R3099 T5793 T5799 nsubjpass antibody,obtained
R3100 T5794 T5793 cc and,antibody
R3101 T5795 T5797 amod anti-CD28,antibody
R3102 T5796 T5797 amod monoclonal,antibody
R3103 T5797 T5793 conj antibody,antibody
R3104 T5798 T5799 auxpass were,obtained
R3105 T5799 T5799 ROOT obtained,obtained
R3106 T5800 T5799 prep from,obtained
R3107 T5801 T5802 compound BD,Biosciences
R3108 T5802 T5800 pobj Biosciences,from
R3109 T5803 T5802 punct (,Biosciences
R3110 T5804 T5805 compound San,Diego
R3111 T5805 T5802 npadvmod Diego,Biosciences
R3112 T5806 T5805 punct ",",Diego
R3113 T5807 T5805 conj CA,Diego
R3114 T5808 T5807 punct ",",CA
R3115 T5809 T5807 conj USA,CA
R3116 T5810 T5802 punct ),Biosciences
R3117 T5811 T5799 punct .,obtained
R3118 T5812 T5822 nsubjpass LY294002,obtained
R3119 T5813 T5812 punct ",",LY294002
R3120 T5814 T5822 nsubjpass SB203580,obtained
R3121 T5815 T5814 punct ",",SB203580
R3122 T5816 T5814 conj FK506,SB203580
R3123 T5817 T5816 punct ",",FK506
R3124 T5818 T5816 conj wortmannin,FK506
R3125 T5819 T5818 cc and,wortmannin
R3126 T5820 T5822 nsubjpass PD98059,obtained
R3127 T5821 T5822 auxpass were,obtained
R3128 T5822 T5822 ROOT obtained,obtained
R3129 T5823 T5822 prep from,obtained
R3130 T5824 T5823 pobj Calbiochem,from
R3131 T5825 T5826 punct (,Schwalbach
R3132 T5826 T5824 appos Schwalbach,Calbiochem
R3133 T5827 T5826 punct ",",Schwalbach
R3134 T5828 T5826 appos Germany,Schwalbach
R3135 T5829 T5826 punct ),Schwalbach
R3136 T5830 T5822 punct .,obtained
R3328 T6294 T6308 nsubjpass Production,prepared
R3329 T6295 T6294 prep of,Production
R3330 T6296 T6295 pobj IL-17,of
R3331 T6297 T6294 prep by,Production
R3332 T6298 T6301 compound T,activation
R3333 T6299 T6300 compound cell,receptor
R3334 T6300 T6301 compound receptor,activation
R3335 T6301 T6297 pobj activation,by
R3336 T6302 T6301 punct ",",activation
R3337 T6303 T6301 conj cytokines,activation
R3338 T6304 T6303 cc or,cytokines
R3339 T6305 T6306 compound chemokines,PBMC
R3340 T6306 T6303 conj PBMC,cytokines
R3341 T6307 T6308 auxpass were,prepared
R3342 T6308 T6308 ROOT prepared,prepared
R3343 T6309 T6308 prep from,prepared
R3344 T6310 T6311 amod heparinized,blood
R3345 T6311 T6309 pobj blood,from
R3346 T6312 T6308 agent by,prepared
R3347 T6313 T6318 nmod Ficoll-Hypaque,centrifugation
R3348 T6314 T6315 punct (,SG1077
R3349 T6315 T6313 appos SG1077,Ficoll-Hypaque
R3350 T6316 T6318 punct ),centrifugation
R3351 T6317 T6318 amod density-gradient,centrifugation
R3352 T6318 T6312 pobj centrifugation,by
R3353 T6319 T6308 punct .,prepared
R3354 T6320 T6321 compound Cell,cultures
R3355 T6321 T6323 nsubjpass cultures,performed
R3356 T6322 T6323 auxpass were,performed
R3357 T6323 T6323 ROOT performed,performed
R3358 T6324 T6325 mark as,described
R3359 T6325 T6323 advcl described,performed
R3360 T6326 T6329 advmod previously,]
R3361 T6327 T6329 nmod [,]
R3362 T6328 T6329 nummod 18,]
R3363 T6329 T6325 dobj ],described
R3364 T6330 T6323 punct .,performed
R3365 T6331 T6338 prep In,adjusted
R3366 T6332 T6331 pobj brief,In
R3367 T6333 T6338 punct ",",adjusted
R3368 T6334 T6336 det the,suspensions
R3369 T6335 T6336 compound cell,suspensions
R3370 T6336 T6338 nsubjpass suspensions,adjusted
R3371 T6337 T6338 auxpass were,adjusted
R3372 T6338 T6338 ROOT adjusted,adjusted
R3373 T6339 T6348 prep to,supplemented
R3374 T6340 T6341 det a,concentration
R3375 T6341 T6339 pobj concentration,to
R3376 T6342 T6341 prep of,concentration
R3377 T6343 T6342 pobj 106/ml,of
R3378 T6344 T6341 prep in,concentration
R3379 T6345 T6348 nsubj RPMI,supplemented
R3380 T6346 T6347 nummod 1640,medium
R3381 T6347 T6348 nsubj medium,supplemented
R3382 T6348 T6338 xcomp supplemented,adjusted
R3383 T6349 T6348 prep with,supplemented
R3384 T6350 T6351 nummod 10,%
R3385 T6351 T6354 compound %,serum
R3386 T6352 T6353 amod fetal,calf
R3387 T6353 T6354 compound calf,serum
R3388 T6354 T6349 pobj serum,with
R3389 T6355 T6354 punct ",",serum
R3390 T6356 T6358 nummod 100,penicillin
R3391 T6357 T6358 compound U/ml,penicillin
R3392 T6358 T6354 conj penicillin,serum
R3393 T6359 T6358 punct ",",penicillin
R3394 T6360 T6362 nummod 100,streptomycin
R3395 T6361 T6362 compound mg/ml,streptomycin
R3396 T6362 T6358 conj streptomycin,penicillin
R3397 T6363 T6362 cc and,streptomycin
R3398 T6364 T6366 nummod 2,L-glutamine
R3399 T6365 T6366 compound mM,L-glutamine
R3400 T6366 T6362 conj L-glutamine,streptomycin
R3401 T6367 T6338 punct .,adjusted
R3402 T6368 T6369 compound Cell,suspension
R3403 T6369 T6375 nsubjpass suspension,dispensed
R3404 T6370 T6372 punct (,ml
R3405 T6371 T6372 nummod 1,ml
R3406 T6372 T6369 appos ml,suspension
R3407 T6373 T6372 punct ),ml
R3408 T6374 T6375 auxpass was,dispensed
R3409 T6375 T6375 ROOT dispensed,dispensed
R3410 T6376 T6375 prep into,dispensed
R3411 T6377 T6379 amod 24-well,plates
R3412 T6378 T6379 amod multi-well,plates
R3413 T6379 T6376 pobj plates,into
R3414 T6380 T6379 punct (,plates
R3415 T6381 T6381 ROOT Nunc,Nunc
R3416 T6382 T6381 punct ",",Nunc
R3417 T6383 T6381 conj Roskilde,Nunc
R3418 T6384 T6383 punct ",",Roskilde
R3419 T6385 T6383 npadvmod Denmark,Roskilde
R3420 T6386 T6383 punct ),Roskilde
R3421 T6387 T6381 punct ",",Nunc
R3422 T6388 T6375 cc and,dispensed
R3423 T6389 T6375 conj incubated,dispensed
R3424 T6390 T6389 prep for,incubated
R3425 T6391 T6392 nummod 24,hours
R3426 T6392 T6390 pobj hours,for
R3427 T6393 T6389 prep at,incubated
R3428 T6394 T6395 nummod 37,°
R3429 T6395 T6393 pobj °,at
R3430 T6396 T6389 conj C,incubated
R3431 T6397 T6389 prep in,incubated
R3432 T6398 T6399 nummod 5,%
R3433 T6399 T6397 pobj %,in
R3434 T6400 T6399 nummod CO2,%
R3435 T6401 T6375 punct .,dispensed
R3436 T6402 T6415 advmod Subsequently,added
R3437 T6403 T6415 punct ",",added
R3438 T6404 T6405 amod various,concentrations
R3439 T6405 T6415 nsubjpass concentrations,added
R3440 T6406 T6405 prep of,concentrations
R3441 T6407 T6408 compound cyclosporin,A
R3442 T6408 T6406 pobj A,of
R3443 T6409 T6408 punct (,A
R3444 T6410 T6408 appos 10,A
R3445 T6411 T6412 nummod 500,ng/ml
R3446 T6412 T6410 appos ng/ml,10
R3447 T6413 T6408 punct ),A
R3448 T6414 T6415 auxpass were,added
R3449 T6415 T6415 ROOT added,added
R3450 T6416 T6415 prep to,added
R3451 T6417 T6418 det the,medium
R3452 T6418 T6416 pobj medium,to
R3453 T6419 T6418 cc and,medium
R3454 T6420 T6418 conj cells,medium
R3455 T6421 T6422 auxpass were,incubated
R3456 T6422 T6422 ROOT incubated,incubated
R3457 T6423 T6422 prep for,incubated
R3458 T6424 T6425 nummod 24,hours
R3459 T6425 T6423 pobj hours,for
R3460 T6426 T6422 punct .,incubated
R3461 T6427 T6431 aux To,added
R3462 T6428 T6429 det each,well
R3463 T6429 T6431 nsubjpass well,added
R3464 T6430 T6431 auxpass was,added
R3465 T6431 T6431 ROOT added,added
R3466 T6432 T6431 dobj FK506,added
R3467 T6433 T6432 punct ",",FK506
R3468 T6434 T6432 appos rapamycin,FK506
R3469 T6435 T6434 punct ",",rapamycin
R3470 T6436 T6434 conj curcumin,rapamycin
R3471 T6437 T6436 punct ",",curcumin
R3472 T6438 T6436 conj PDTC,curcumin
R3473 T6439 T6438 punct ",",PDTC
R3474 T6440 T6438 conj LY294002,PDTC
R3475 T6441 T6440 punct ",",LY294002
R3476 T6442 T6440 conj SB203580,LY294002
R3477 T6443 T6442 punct ",",SB203580
R3478 T6444 T6442 conj PD98059,SB203580
R3479 T6445 T6444 punct ",",PD98059
R3480 T6446 T6444 conj dexamethasone,PD98059
R3481 T6447 T6446 cc or,dexamethasone
R3482 T6448 T6446 conj wortmannin,dexamethasone
R3483 T6449 T6431 punct .,added
R3484 T6450 T6464 prep After,collected
R3485 T6451 T6450 pobj incubation,After
R3486 T6452 T6451 prep for,incubation
R3487 T6453 T6454 nummod 24,hours
R3488 T6454 T6452 pobj hours,for
R3489 T6455 T6458 punct (,stated
R3490 T6456 T6458 mark unless,stated
R3491 T6457 T6458 advmod otherwise,stated
R3492 T6458 T6464 advcl stated,collected
R3493 T6459 T6458 punct ),stated
R3494 T6460 T6464 punct ",",collected
R3495 T6461 T6462 amod cell-free,media
R3496 T6462 T6464 nsubjpass media,collected
R3497 T6463 T6464 auxpass were,collected
R3498 T6464 T6464 ROOT collected,collected
R3499 T6465 T6464 cc and,collected
R3500 T6466 T6464 conj stored,collected
R3501 T6467 T6466 prep at,stored
R3502 T6468 T6469 nummod -20,°
R3503 T6469 T6470 nummod °,C
R3504 T6470 T6467 pobj C,at
R3505 T6471 T6472 mark until,assayed
R3506 T6472 T6466 advcl assayed,stored
R3507 T6473 T6464 punct .,collected
R3508 T6474 T6475 det All,cultures
R3509 T6475 T6477 nsubjpass cultures,set
R3510 T6476 T6477 auxpass were,set
R3511 T6477 T6477 ROOT set,set
R3512 T6478 T6477 prt up,set
R3513 T6479 T6477 prep in,set
R3514 T6480 T6479 pobj triplicate,in
R3515 T6481 T6477 punct ",",set
R3516 T6482 T6477 cc and,set
R3517 T6483 T6485 nsubjpass results,expressed
R3518 T6484 T6485 auxpass are,expressed
R3519 T6485 T6477 conj expressed,set
R3520 T6486 T6485 prep as,expressed
R3521 T6487 T6486 pobj means,as
R3522 T6488 T6489 compound ±,SEM
R3523 T6489 T6487 dobj SEM,means
R3524 T6490 T6485 punct .,expressed
R3721 T6978 T6981 nummod CD4,isolation
R3722 T6979 T6981 nummod +,isolation
R3723 T6980 T6981 compound T-cell,isolation
R3724 T6981 T6987 nsubjpass isolation,used
R3725 T6982 T6981 prep by,isolation
R3726 T6983 T6982 pobj MACS,by
R3727 T6984 T6985 amod Anti-CD4,microbeads
R3728 T6985 T6987 nsubjpass microbeads,used
R3729 T6986 T6987 auxpass were,used
R3730 T6987 T6987 ROOT used,used
R3731 T6988 T6990 advmod essentially,recommended
R3732 T6989 T6990 mark as,recommended
R3733 T6990 T6987 advcl recommended,used
R3734 T6991 T6990 agent by,recommended
R3735 T6992 T6993 det the,manufacturer
R3736 T6993 T6991 pobj manufacturer,by
R3737 T6994 T6993 punct (,manufacturer
R3738 T6995 T6993 appos Miltenyi,manufacturer
R3739 T6996 T6993 punct ),manufacturer
R3740 T6997 T6999 nmod [,]
R3741 T6998 T6999 nummod 19,]
R3742 T6999 T6990 dobj ],recommended
R3743 T7000 T6987 punct .,used
R3744 T7001 T7003 nsubjpass PBMC,resuspended
R3745 T7002 T7003 auxpass were,resuspended
R3746 T7003 T7003 ROOT resuspended,resuspended
R3747 T7004 T7003 prep in,resuspended
R3748 T7005 T7006 nummod 80,μl
R3749 T7006 T7004 pobj μl,in
R3750 T7007 T7006 prep of,μl
R3751 T7008 T7007 pobj FBS,of
R3752 T7009 T7003 advcl staining,resuspended
R3753 T7010 T7009 dobj buffer,staining
R3754 T7011 T7003 punct .,resuspended
R3755 T7012 T7013 amod Anti-CD4,microbeads
R3756 T7013 T7019 nsubjpass microbeads,added
R3757 T7014 T7013 punct (,microbeads
R3758 T7015 T7016 nummod 20,μl
R3759 T7016 T7013 appos μl,microbeads
R3760 T7017 T7013 punct ),microbeads
R3761 T7018 T7019 auxpass were,added
R3762 T7019 T7019 ROOT added,added
R3763 T7020 T7019 cc and,added
R3764 T7021 T7019 conj incubated,added
R3765 T7022 T7021 prep for,incubated
R3766 T7023 T7024 nummod 15,min
R3767 T7024 T7022 pobj min,for
R3768 T7025 T7024 prep at,min
R3769 T7026 T7025 pobj 6,at
R3770 T7027 T7028 nummod 12,°
R3771 T7028 T7030 compound °,Saturating
R3772 T7029 T7030 compound C.,Saturating
R3773 T7030 T7031 nummod Saturating,amounts
R3774 T7031 T7036 nsubjpass amounts,added
R3775 T7032 T7031 prep of,amounts
R3776 T7033 T7034 amod fluorochrome-conjugated,antibodies
R3777 T7034 T7032 pobj antibodies,of
R3778 T7035 T7036 auxpass were,added
R3779 T7036 T7019 conj added,added
R3780 T7037 T7036 prep for,added
R3781 T7038 T7041 det a,min
R3782 T7039 T7041 amod further,min
R3783 T7040 T7041 nummod 10,min
R3784 T7041 T7037 pobj min,for
R3785 T7042 T7036 punct .,added
R3786 T7043 T7045 nsubjpass Cells,diluted
R3787 T7044 T7045 auxpass were,diluted
R3788 T7045 T7045 ROOT diluted,diluted
R3789 T7046 T7045 prep in,diluted
R3790 T7047 T7048 nummod 2.5,ml
R3791 T7048 T7046 pobj ml,in
R3792 T7049 T7048 prep of,ml
R3793 T7050 T7049 pobj FBS,of
R3794 T7051 T7052 compound staining,buffer
R3795 T7052 T7048 conj buffer,ml
R3796 T7053 T7048 punct ",",ml
R3797 T7054 T7056 amod pelleted,resuspended
R3798 T7055 T7056 punct ",",resuspended
R3799 T7056 T7045 conj resuspended,diluted
R3800 T7057 T7056 prep in,resuspended
R3801 T7058 T7059 nummod 500,μl
R3802 T7059 T7057 pobj μl,in
R3803 T7060 T7056 cc and,resuspended
R3804 T7061 T7062 advmod magnetically,separated
R3805 T7062 T7056 conj separated,resuspended
R3806 T7063 T7062 punct ",",separated
R3807 T7064 T7065 advmod usually,on
R3808 T7065 T7062 prep on,separated
R3809 T7066 T7068 det an,magnet
R3810 T7067 T7068 compound AutoMACS,magnet
R3811 T7068 T7065 pobj magnet,on
R3812 T7069 T7068 acl fitted,magnet
R3813 T7070 T7069 prep with,fitted
R3814 T7071 T7074 det a,column
R3815 T7072 T7074 compound MACS,column
R3816 T7073 T7074 compound MS,column
R3817 T7074 T7070 pobj column,with
R3818 T7075 T7045 punct .,diluted
R3819 T7076 T7076 ROOT Flow-through,Flow-through
R3820 T7077 T7076 cc and,Flow-through
R3821 T7078 T7081 nummod two,washes
R3822 T7079 T7080 nummod 1,ml
R3823 T7080 T7081 compound ml,washes
R3824 T7081 T7083 nsubjpass washes,collected
R3825 T7082 T7083 auxpass were,collected
R3826 T7083 T7076 conj collected,Flow-through
R3827 T7084 T7083 prep as,collected
R3828 T7085 T7087 det the,fraction
R3829 T7086 T7087 amod negative,fraction
R3830 T7087 T7084 pobj fraction,as
R3831 T7088 T7083 punct .,collected
R3832 T7089 T7090 amod Enriched,cells
R3833 T7090 T7092 nsubjpass cells,collected
R3834 T7091 T7092 auxpass were,collected
R3835 T7092 T7092 ROOT collected,collected
R3836 T7093 T7092 prep in,collected
R3837 T7094 T7097 nummod two,aliquots
R3838 T7095 T7096 nummod 0.5,ml
R3839 T7096 T7097 compound ml,aliquots
R3840 T7097 T7093 pobj aliquots,in
R3841 T7098 T7097 prep from,aliquots
R3842 T7099 T7100 det the,column
R3843 T7100 T7098 pobj column,from
R3844 T7101 T7100 prep after,column
R3845 T7102 T7101 pobj removal,after
R3846 T7103 T7102 prep from,removal
R3847 T7104 T7105 det the,magnet
R3848 T7105 T7103 pobj magnet,from
R3849 T7106 T7092 punct .,collected
R3850 T7107 T7110 advmod Alternatively,stained
R3851 T7108 T7110 punct ",",stained
R3852 T7109 T7110 nsubj cells,stained
R3853 T7110 T7110 ROOT stained,stained
R3854 T7111 T7110 prep with,stained
R3855 T7112 T7111 pobj anti-CD4,with
R3856 T7113 T7115 nsubjpass phycoerythrin,washed
R3857 T7114 T7115 auxpass were,washed
R3858 T7115 T7115 ROOT washed,washed
R3859 T7116 T7115 punct ",",washed
R3860 T7117 T7118 advmod magnetically,labeled
R3861 T7118 T7115 conj labeled,washed
R3862 T7119 T7118 prep with,labeled
R3863 T7120 T7121 amod anti-phycoerythrin,microbeads
R3864 T7121 T7119 pobj microbeads,with
R3865 T7122 T7125 punct (,added
R3866 T7123 T7124 nummod 20,μl
R3867 T7124 T7125 nsubj μl,added
R3868 T7125 T7125 ROOT added,added
R3869 T7126 T7125 prep to,added
R3870 T7127 T7128 nummod 80,μl
R3871 T7128 T7126 pobj μl,to
R3872 T7129 T7128 prep of,μl
R3873 T7130 T7131 compound cell,suspension
R3874 T7131 T7129 pobj suspension,of
R3875 T7132 T7125 punct ;,added
R3876 T7133 T7134 nummod 15,min
R3877 T7134 T7134 ROOT min,min
R3878 T7135 T7134 punct ",",min
R3879 T7136 T7134 appos 6,min
R3880 T7137 T7138 nummod 12,°
R3881 T7138 T7139 compound °,C
R3882 T7139 T7134 appos C,min
R3883 T7140 T7134 punct ),min
R3884 T7141 T7134 punct ",",min
R3885 T7142 T7134 cc and,min
R3886 T7143 T7144 advmod magnetically,separated
R3887 T7144 T7134 conj separated,min
R3888 T7145 T7146 mark as,described
R3889 T7146 T7144 advcl described,separated
R3890 T7147 T7146 advmod above,described
R3891 T7148 T7125 punct .,added
R3892 T7149 T7150 det The,purity
R3893 T7150 T7154 nsubjpass purity,assessed
R3894 T7151 T7150 prep of,purity
R3895 T7152 T7151 pobj cells,of
R3896 T7153 T7154 auxpass was,assessed
R3897 T7154 T7154 ROOT assessed,assessed
R3898 T7155 T7154 agent by,assessed
R3899 T7156 T7155 pobj flow,by
R3900 T7157 T7158 amod cytometric,analysis
R3901 T7158 T7154 npadvmod analysis,assessed
R3902 T7159 T7158 prep of,analysis
R3903 T7160 T7161 amod stained,cells
R3904 T7161 T7159 pobj cells,of
R3905 T7162 T7158 prep on,analysis
R3906 T7163 T7166 det a,sorter
R3907 T7164 T7166 compound FACS,sorter
R3908 T7165 T7166 compound Vantage,sorter
R3909 T7166 T7162 pobj sorter,on
R3910 T7167 T7154 punct .,assessed
R3911 T7168 T7179 nsubj Most,had
R3912 T7169 T7173 punct (,%
R3913 T7170 T7172 amod more,97
R3914 T7171 T7172 quantmod than,97
R3915 T7172 T7173 nummod 97,%
R3916 T7173 T7168 appos %,Most
R3917 T7174 T7173 punct ),%
R3918 T7175 T7173 prep of,%
R3919 T7176 T7178 det the,cells
R3920 T7177 T7178 amod isolated,cells
R3921 T7178 T7175 pobj cells,of
R3922 T7179 T7179 ROOT had,had
R3923 T7180 T7184 det the,marker
R3924 T7181 T7182 compound CD4,T
R3925 T7182 T7184 compound T,marker
R3926 T7183 T7184 compound cell,marker
R3927 T7184 T7179 dobj marker,had
R3928 T7185 T7179 punct .,had
R4194 T7843 T7845 amod Enzyme-linked,assay
R4195 T7844 T7845 amod immunosorbent,assay
R4196 T7845 T7853 nsubjpass assay,measured
R4197 T7846 T7845 prep of,assay
R4198 T7847 T7848 compound IL-17,IL-17
R4199 T7848 T7846 pobj IL-17,of
R4200 T7849 T7845 prep in,assay
R4201 T7850 T7851 compound culture,supernatants
R4202 T7851 T7849 pobj supernatants,in
R4203 T7852 T7853 auxpass was,measured
R4204 T7853 T7853 ROOT measured,measured
R4205 T7854 T7853 agent by,measured
R4206 T7855 T7858 nmod sandwich,assay
R4207 T7856 T7858 amod enzyme-linked,assay
R4208 T7857 T7858 compound immunosorbent,assay
R4209 T7858 T7854 pobj assay,by
R4210 T7859 T7860 mark as,described
R4211 T7860 T7853 advcl described,measured
R4212 T7861 T7864 advmod previously,]
R4213 T7862 T7864 nmod [,]
R4214 T7863 T7864 nummod 20,]
R4215 T7864 T7860 dobj ],described
R4216 T7865 T7853 punct .,measured
R4217 T7866 T7876 prep In,coated
R4218 T7867 T7866 pobj brief,In
R4219 T7868 T7876 punct ",",coated
R4220 T7869 T7871 det a,plate
R4221 T7870 T7871 amod 96-well,plate
R4222 T7871 T7876 nsubjpass plate,coated
R4223 T7872 T7873 punct (,Nunc
R4224 T7873 T7871 appos Nunc,plate
R4225 T7874 T7873 punct ),Nunc
R4226 T7875 T7876 auxpass was,coated
R4227 T7876 T7876 ROOT coated,coated
R4228 T7877 T7876 prep with,coated
R4229 T7878 T7879 nummod 4,μg
R4230 T7879 T7883 amod μg,antibodies
R4231 T7880 T7883 nmod /,antibodies
R4232 T7881 T7883 amod ml,antibodies
R4233 T7882 T7883 amod monoclonal,antibodies
R4234 T7883 T7877 pobj antibodies,with
R4235 T7884 T7883 prep against,antibodies
R4236 T7885 T7884 pobj IL-17,against
R4237 T7886 T7885 punct (,IL-17
R4238 T7887 T7885 conj R,IL-17
R4239 T7888 T7887 cc &,R
R4240 T7889 T7890 compound D,Systems
R4241 T7890 T7887 conj Systems,R
R4242 T7891 T7885 punct ),IL-17
R4243 T7892 T7876 prep at,coated
R4244 T7893 T7894 nummod 4,°
R4245 T7894 T7895 nummod °,C
R4246 T7895 T7896 compound C,overnight
R4247 T7896 T7892 pobj overnight,at
R4248 T7897 T7876 punct .,coated
R4249 T7898 T7910 prep After,/
R4250 T7899 T7898 pcomp blocking,After
R4251 T7900 T7899 prep with,blocking
R4252 T7901 T7906 amod phosphate-buffered,albumin
R4253 T7902 T7903 nummod saline/1,%
R4254 T7903 T7906 compound %,albumin
R4255 T7904 T7905 amod bovine,serum
R4256 T7905 T7906 compound serum,albumin
R4257 T7906 T7900 pobj albumin,with
R4258 T7907 T7908 punct (,BSA
R4259 T7908 T7906 npadvmod BSA,albumin
R4260 T7909 T7910 punct ),/
R4261 T7910 T7910 ROOT /,/
R4262 T7911 T7912 nummod 0.05,%
R4263 T7912 T7929 nmod %,samples
R4264 T7913 T7914 nummod Tween,20
R4265 T7914 T7929 nummod 20,samples
R4266 T7915 T7914 prep for,20
R4267 T7916 T7917 nummod 2,hours
R4268 T7917 T7915 pobj hours,for
R4269 T7918 T7917 prep at,hours
R4270 T7919 T7920 compound room,temperature
R4271 T7920 T7918 pobj temperature,at
R4272 T7921 T7917 punct (,hours
R4273 T7922 T7917 nummod 22,hours
R4274 T7923 T7925 nummod 25,C
R4275 T7924 T7925 compound °,C
R4276 T7925 T7917 appos C,hours
R4277 T7926 T7914 punct ),20
R4278 T7927 T7914 punct ",",20
R4279 T7928 T7929 compound test,samples
R4280 T7929 T7910 dobj samples,/
R4281 T7930 T7929 cc and,samples
R4282 T7931 T7934 det the,IL-17
R4283 T7932 T7934 amod standard,IL-17
R4284 T7933 T7934 compound recombinant,IL-17
R4285 T7934 T7929 conj IL-17,samples
R4286 T7935 T7942 punct (,added
R4287 T7936 T7942 nsubjpass R,added
R4288 T7937 T7936 cc &,R
R4289 T7938 T7939 compound D,Systems
R4290 T7939 T7936 conj Systems,R
R4291 T7940 T7942 punct ),added
R4292 T7941 T7942 auxpass were,added
R4293 T7942 T7934 parataxis added,IL-17
R4294 T7943 T7942 prep to,added
R4295 T7944 T7946 det the,plate
R4296 T7945 T7946 amod 96-well,plate
R4297 T7946 T7943 pobj plate,to
R4298 T7947 T7942 cc and,added
R4299 T7948 T7942 conj incubated,added
R4300 T7949 T7948 prep at,incubated
R4301 T7950 T7951 compound room,temperature
R4302 T7951 T7949 pobj temperature,at
R4303 T7952 T7948 prep for,incubated
R4304 T7953 T7954 nummod 2,hours
R4305 T7954 T7952 pobj hours,for
R4306 T7955 T7942 punct .,added
R4307 T7956 T7958 nsubjpass Plates,washed
R4308 T7957 T7958 auxpass were,washed
R4309 T7958 T7958 ROOT washed,washed
R4310 T7959 T7960 nummod four,times
R4311 T7960 T7958 npadvmod times,washed
R4312 T7961 T7958 prep with,washed
R4313 T7962 T7963 compound phosphate-buffered,saline/Tween
R4314 T7963 T7961 pobj saline/Tween,with
R4315 T7964 T7963 nummod 20,saline/Tween
R4316 T7965 T7958 punct ",",washed
R4317 T7966 T7958 cc and,washed
R4318 T7967 T7968 advmod then,incubated
R4319 T7968 T7958 conj incubated,washed
R4320 T7969 T7968 prep with,incubated
R4321 T7970 T7975 nummod 500,antibodies
R4322 T7971 T7975 amod ng/ml,antibodies
R4323 T7972 T7975 amod biotinylated,antibodies
R4324 T7973 T7975 compound mouse,antibodies
R4325 T7974 T7975 compound monoclonal,antibodies
R4326 T7975 T7969 pobj antibodies,with
R4327 T7976 T7975 prep against,antibodies
R4328 T7977 T7976 pobj IL-17,against
R4329 T7978 T7977 punct (,IL-17
R4330 T7979 T7977 conj R,IL-17
R4331 T7980 T7979 cc &,R
R4332 T7981 T7982 compound D,Systems
R4333 T7982 T7979 conj Systems,R
R4334 T7983 T7968 punct ),incubated
R4335 T7984 T7968 prep for,incubated
R4336 T7985 T7986 nummod 2,hours
R4337 T7986 T7984 pobj hours,for
R4338 T7987 T7986 prep at,hours
R4339 T7988 T7989 compound room,temperature
R4340 T7989 T7987 pobj temperature,at
R4341 T7990 T7958 punct .,washed
R4342 T7991 T8004 prep After,incubated
R4343 T7992 T7991 pcomp washing,After
R4344 T7993 T8004 punct ",",incubated
R4345 T7994 T8004 nsubjpass streptavidin,incubated
R4346 T7995 T7996 amod alkaline,phosphate
R4347 T7996 T8003 nsubj phosphate,was
R4348 T7997 T7999 amod horseradish,conjugate
R4349 T7998 T7999 amod peroxidase,conjugate
R4350 T7999 T7996 appos conjugate,phosphate
R4351 T8000 T8001 punct (,Sigma
R4352 T8001 T7999 appos Sigma,conjugate
R4353 T8002 T7999 punct ),conjugate
R4354 T8003 T8004 auxpass was,incubated
R4355 T8004 T8004 ROOT incubated,incubated
R4356 T8005 T8004 prep for,incubated
R4357 T8006 T8007 nummod 2,hours
R4358 T8007 T8005 pobj hours,for
R4359 T8008 T8010 punct ",",washed
R4360 T8009 T8010 advmod then,washed
R4361 T8010 T8004 dep washed,incubated
R4362 T8011 T8010 advmod again,washed
R4363 T8012 T8010 cc and,washed
R4364 T8013 T8010 conj incubated,washed
R4365 T8014 T8013 prep with,incubated
R4366 T8015 T8016 nummod 1,mg/ml
R4367 T8016 T8018 nmod mg/ml,phosphate
R4368 T8017 T8018 compound p-nitrophenyl,phosphate
R4369 T8018 T8014 pobj phosphate,with
R4370 T8019 T8020 punct (,Sigma
R4371 T8020 T8018 appos Sigma,phosphate
R4372 T8021 T8013 punct ),incubated
R4373 T8022 T8010 conj dissolved,washed
R4374 T8023 T8022 prep in,dissolved
R4375 T8024 T8023 pobj diethanolamine,in
R4376 T8025 T8026 punct (,Sigma
R4377 T8026 T8024 appos Sigma,diethanolamine
R4378 T8027 T8024 punct ),diethanolamine
R4379 T8028 T8029 aux to,develop
R4380 T8029 T8022 advcl develop,dissolved
R4381 T8030 T8032 det the,reaction
R4382 T8031 T8032 compound color,reaction
R4383 T8032 T8029 dobj reaction,develop
R4384 T8033 T8004 punct .,incubated
R4385 T8034 T8035 det The,reaction
R4386 T8035 T8037 nsubjpass reaction,stopped
R4387 T8036 T8037 auxpass was,stopped
R4388 T8037 T8037 ROOT stopped,stopped
R4389 T8038 T8037 agent by,stopped
R4390 T8039 T8040 det the,addition
R4391 T8040 T8038 pobj addition,by
R4392 T8041 T8040 prep of,addition
R4393 T8042 T8044 nummod 1,NaOH
R4394 T8043 T8044 compound M,NaOH
R4395 T8044 T8041 pobj NaOH,of
R4396 T8045 T8044 cc and,NaOH
R4397 T8046 T8048 det the,density
R4398 T8047 T8048 amod optical,density
R4399 T8048 T8044 conj density,NaOH
R4400 T8049 T8048 prep of,density
R4401 T8050 T8051 det each,well
R4402 T8051 T8049 pobj well,of
R4403 T8052 T8053 auxpass was,read
R4404 T8053 T8037 conj read,stopped
R4405 T8054 T8053 prep at,read
R4406 T8055 T8056 nummod 405,nm
R4407 T8056 T8054 pobj nm,at
R4408 T8057 T8053 punct .,read
R4409 T8058 T8060 det The,limit
R4410 T8059 T8060 amod lower,limit
R4411 T8060 T8064 nsubj limit,was
R4412 T8061 T8060 prep of,limit
R4413 T8062 T8063 compound IL-17,detection
R4414 T8063 T8061 pobj detection,of
R4415 T8064 T8064 ROOT was,was
R4416 T8065 T8066 nummod 10,pg/ml
R4417 T8066 T8064 attr pg/ml,was
R4418 T8067 T8064 punct .,was
R4419 T8068 T8070 amod Recombinant,IL-17
R4420 T8069 T8070 amod human,IL-17
R4421 T8070 T8071 nsubj IL-17,diluted
R4422 T8071 T8076 nsubjpass diluted,used
R4423 T8072 T8071 prep in,diluted
R4424 T8073 T8074 compound culture,medium
R4425 T8074 T8072 pobj medium,in
R4426 T8075 T8076 auxpass was,used
R4427 T8076 T8076 ROOT used,used
R4428 T8077 T8076 prep as,used
R4429 T8078 T8080 det a,standard
R4430 T8079 T8080 compound calibration,standard
R4431 T8080 T8077 pobj standard,as
R4432 T8081 T8076 punct ",",used
R4433 T8082 T8076 advcl ranging,used
R4434 T8083 T8082 prep from,ranging
R4435 T8084 T8083 pobj 10,from
R4436 T8085 T8083 prep to,from
R4437 T8086 T8087 det 2000,pg/ml
R4438 T8087 T8085 pobj pg/ml,to
R4439 T8088 T8076 punct .,used
R4440 T8089 T8091 det A,curve
R4441 T8090 T8091 amod standard,curve
R4442 T8091 T8093 nsubjpass curve,drawn
R4443 T8092 T8093 auxpass was,drawn
R4444 T8093 T8093 ROOT drawn,drawn
R4445 T8094 T8093 agent by,drawn
R4446 T8095 T8094 pcomp plotting,by
R4447 T8096 T8097 amod optical,density
R4448 T8097 T8095 dobj density,plotting
R4449 T8098 T8097 prep against,density
R4450 T8099 T8100 det the,log
R4451 T8100 T8098 pobj log,against
R4452 T8101 T8100 prep of,log
R4453 T8102 T8103 det the,concentration
R4454 T8103 T8101 pobj concentration,of
R4455 T8104 T8103 prep of,concentration
R4456 T8105 T8106 amod recombinant,cytokines
R4457 T8106 T8104 pobj cytokines,of
R4458 T8107 T8093 punct ",",drawn
R4459 T8108 T8093 cc and,drawn
R4460 T8109 T8093 conj used,drawn
R4461 T8110 T8109 prep for,used
R4462 T8111 T8110 pobj determination,for
R4463 T8112 T8111 prep of,determination
R4464 T8113 T8112 pobj IL-17,of
R4465 T8114 T8111 prep in,determination
R4466 T8115 T8116 compound test,samples
R4467 T8116 T8114 pobj samples,in
R4468 T8117 T8093 punct .,drawn
R4487 T8766 T8779 nsubjpass Quantification,incubated
R4488 T8767 T8766 prep of,Quantification
R4489 T8768 T8769 compound IL-17,mRNA
R4490 T8769 T8767 pobj mRNA,of
R4491 T8770 T8766 prep by,Quantification
R4492 T8771 T8773 amod semiquantitative,transcription
R4493 T8772 T8773 compound reverse,transcription
R4494 T8773 T8770 pobj transcription,by
R4495 T8774 T8776 compound polymerase,reaction
R4496 T8775 T8776 compound chain,reaction
R4497 T8776 T8777 compound reaction,PBMC
R4498 T8777 T8766 conj PBMC,Quantification
R4499 T8778 T8779 auxpass were,incubated
R4500 T8779 T8779 ROOT incubated,incubated
R4501 T8780 T8779 prep with,incubated
R4502 T8781 T8782 amod various,concentrations
R4503 T8782 T8780 pobj concentrations,with
R4504 T8783 T8782 prep of,concentrations
R4505 T8784 T8783 pobj anti-CD3,of
R4506 T8785 T8782 prep in,concentrations
R4507 T8786 T8787 det the,presence
R4508 T8787 T8785 pobj presence,in
R4509 T8788 T8787 cc or,presence
R4510 T8789 T8787 conj absence,presence
R4511 T8790 T8789 prep of,absence
R4512 T8791 T8790 pobj inhibitors,of
R4513 T8792 T8791 punct (,inhibitors
R4514 T8793 T8791 appos LY294002,inhibitors
R4515 T8794 T8791 punct ",",inhibitors
R4516 T8795 T8791 appos PDTC,inhibitors
R4517 T8796 T8791 punct ),inhibitors
R4518 T8797 T8779 punct .,incubated
R4519 T8798 T8806 prep After,extracted
R4520 T8799 T8800 nummod 16,hours
R4521 T8800 T8798 pobj hours,After
R4522 T8801 T8800 prep of,hours
R4523 T8802 T8801 pobj incubation,of
R4524 T8803 T8806 punct ",",extracted
R4525 T8804 T8806 nsubjpass mRNA,extracted
R4526 T8805 T8806 auxpass was,extracted
R4527 T8806 T8806 ROOT extracted,extracted
R4528 T8807 T8806 prep with,extracted
R4529 T8808 T8809 compound RNAzol,B
R4530 T8809 T8807 pobj B,with
R4531 T8810 T8812 punct (,Laboratories
R4532 T8811 T8812 compound Biotex,Laboratories
R4533 T8812 T8809 appos Laboratories,B
R4534 T8813 T8812 punct ",",Laboratories
R4535 T8814 T8812 conj Houston,Laboratories
R4536 T8815 T8814 punct ",",Houston
R4537 T8816 T8814 conj TX,Houston
R4538 T8817 T8816 punct ",",TX
R4539 T8818 T8816 conj USA,TX
R4540 T8819 T8812 punct ),Laboratories
R4541 T8820 T8806 prep in,extracted
R4542 T8821 T8820 pobj accordance,in
R4543 T8822 T8821 prep with,accordance
R4544 T8823 T8824 det the,manufacturer
R4545 T8824 T8826 poss manufacturer,instructions
R4546 T8825 T8824 case 's,manufacturer
R4547 T8826 T8822 pobj instructions,with
R4548 T8827 T8806 punct .,extracted
R4549 T8828 T8828 ROOT Reverse,Reverse
R4550 T8829 T8837 nsubjpass transcription,performed
R4551 T8830 T8829 prep of,transcription
R4552 T8831 T8832 nummod 2,μg
R4553 T8832 T8830 pobj μg,of
R4554 T8833 T8832 prep of,μg
R4555 T8834 T8835 amod total,mRNA
R4556 T8835 T8833 pobj mRNA,of
R4557 T8836 T8837 auxpass was,performed
R4558 T8837 T8828 ccomp performed,Reverse
R4559 T8838 T8837 prep at,performed
R4560 T8839 T8840 nummod 42,°
R4561 T8840 T8841 nummod °,C
R4562 T8841 T8838 pobj C,at
R4563 T8842 T8837 advcl using,performed
R4564 T8843 T8848 det the,system
R4565 T8844 T8848 nmod Superscript,system
R4566 T8845 T8848 compound ™,system
R4567 T8846 T8848 compound reverse,system
R4568 T8847 T8848 compound transcription,system
R4569 T8848 T8842 dobj system,using
R4570 T8849 T8848 punct (,system
R4571 T8850 T8848 appos Takara,system
R4572 T8851 T8850 punct ",",Takara
R4573 T8852 T8850 conj Shiga,Takara
R4574 T8853 T8852 punct ",",Shiga
R4575 T8854 T8852 npadvmod Japan,Shiga
R4576 T8855 T8852 punct ),Shiga
R4577 T8856 T8828 punct .,Reverse
R4578 T8857 T8858 compound PCR,amplification
R4579 T8858 T8863 nsubjpass amplification,performed
R4580 T8859 T8858 prep of,amplification
R4581 T8860 T8861 compound cDNA,aliquots
R4582 T8861 T8859 pobj aliquots,of
R4583 T8862 T8863 auxpass was,performed
R4584 T8863 T8863 ROOT performed,performed
R4585 T8864 T8863 prep by,performed
R4586 T8865 T8864 pcomp adding,by
R4587 T8866 T8868 nummod 2.5,dNTPs
R4588 T8867 T8868 compound mM,dNTPs
R4589 T8868 T8865 dobj dNTPs,adding
R4590 T8869 T8871 punct ",",U
R4591 T8870 T8871 nummod 2.5,U
R4592 T8871 T8868 appos U,dNTPs
R4593 T8872 T8871 prep of,U
R4594 T8873 T8875 compound Taq,polymerase
R4595 T8874 T8875 compound DNA,polymerase
R4596 T8875 T8872 pobj polymerase,of
R4597 T8876 T8877 punct (,Takara
R4598 T8877 T8875 appos Takara,polymerase
R4599 T8878 T8871 punct ),U
R4600 T8879 T8871 cc and,U
R4601 T8880 T8881 nummod 0.25,μM
R4602 T8881 T8871 conj μM,U
R4603 T8882 T8881 prep of,μM
R4604 T8883 T8882 pobj sense,of
R4605 T8884 T8883 cc and,sense
R4606 T8885 T8886 amod antisense,primers
R4607 T8886 T8883 conj primers,sense
R4608 T8887 T8863 punct .,performed
R4609 T8888 T8889 det The,reaction
R4610 T8889 T8891 nsubjpass reaction,performed
R4611 T8890 T8891 auxpass was,performed
R4612 T8891 T8891 ROOT performed,performed
R4613 T8892 T8891 prep in,performed
R4614 T8893 T8894 compound PCR,buffer
R4615 T8894 T8892 pobj buffer,in
R4616 T8895 T8898 punct (,MgCl2
R4617 T8896 T8898 nummod 1.5,MgCl2
R4618 T8897 T8898 compound mM,MgCl2
R4619 T8898 T8894 appos MgCl2,buffer
R4620 T8899 T8898 punct ",",MgCl2
R4621 T8900 T8902 nummod 50,KCl
R4622 T8901 T8902 compound mM,KCl
R4623 T8902 T8898 appos KCl,MgCl2
R4624 T8903 T8902 punct ",",KCl
R4625 T8904 T8906 nummod 10,Tris-HCl
R4626 T8905 T8906 compound mM,Tris-HCl
R4627 T8906 T8902 conj Tris-HCl,KCl
R4628 T8907 T8906 punct ",",Tris-HCl
R4629 T8908 T8906 appos pH,Tris-HCl
R4630 T8909 T8908 nummod 8.3,pH
R4631 T8910 T8906 punct ),Tris-HCl
R4632 T8911 T8891 prep in,performed
R4633 T8912 T8914 det a,volume
R4634 T8913 T8914 amod total,volume
R4635 T8914 T8911 pobj volume,in
R4636 T8915 T8914 prep of,volume
R4637 T8916 T8917 nummod 25,μl
R4638 T8917 T8915 pobj μl,of
R4639 T8918 T8891 punct .,performed
R4640 T8919 T8921 det The,sense
R4641 T8920 T8921 amod following,sense
R4642 T8921 T8929 nsubjpass sense,used
R4643 T8922 T8921 cc and,sense
R4644 T8923 T8924 amod antisense,primers
R4645 T8924 T8921 conj primers,sense
R4646 T8925 T8924 prep for,primers
R4647 T8926 T8927 det each,molecules
R4648 T8927 T8925 pobj molecules,for
R4649 T8928 T8929 auxpass were,used
R4650 T8929 T8929 ROOT used,used
R4651 T8930 T8932 punct :,sense
R4652 T8931 T8932 amod IL-17,sense
R4653 T8932 T8929 dobj sense,used
R4654 T8933 T8932 punct ",",sense
R4655 T8934 T8944 nummod 5,TTG-3
R4656 T8935 T8937 punct ',ATG
R4657 T8936 T8937 punct -,ATG
R4658 T8937 T8944 compound ATG,TTG-3
R4659 T8938 T8944 compound ACT,TTG-3
R4660 T8939 T8941 compound CCT,AAG
R4661 T8940 T8941 compound GGG,AAG
R4662 T8941 T8944 compound AAG,TTG-3
R4663 T8942 T8944 compound ACC,TTG-3
R4664 T8943 T8944 compound TCA,TTG-3
R4665 T8944 T8932 appos TTG-3,sense
R4666 T8945 T8944 case ',TTG-3
R4667 T8946 T8932 punct ;,sense
R4668 T8947 T8948 compound IL-17,antisense
R4669 T8948 T8932 conj antisense,sense
R4670 T8949 T8948 punct ",",antisense
R4671 T8950 T8948 appos 5,antisense
R4672 T8951 T8950 punct ',5
R4673 T8952 T8954 punct -,GGC
R4674 T8953 T8954 compound TTA,GGC
R4675 T8954 T8960 compound GGC,CGG-3
R4676 T8955 T8960 compound CAC,CGG-3
R4677 T8956 T8960 compound ATG,CGG-3
R4678 T8957 T8960 compound GTG,CGG-3
R4679 T8958 T8960 compound GAC,CGG-3
R4680 T8959 T8960 compound AAT,CGG-3
R4681 T8960 T8948 appos CGG-3,antisense
R4682 T8961 T8948 punct ',antisense
R4683 T8962 T8948 punct ;,antisense
R4684 T8963 T8964 amod glyceraldehyde-3-phosphate,dehydrogenase
R4685 T8964 T8948 appos dehydrogenase,antisense
R4686 T8965 T8966 punct (,GAPDH
R4687 T8966 T8964 appos GAPDH,dehydrogenase
R4688 T8967 T8966 punct ),GAPDH
R4689 T8968 T8948 conj sense,antisense
R4690 T8969 T8948 punct ",",antisense
R4691 T8970 T8948 appos 5,antisense
R4692 T8971 T8970 punct ',5
R4693 T8972 T8979 punct -,C-3
R4694 T8973 T8979 compound CGA,C-3
R4695 T8974 T8979 compound TGC,C-3
R4696 T8975 T8979 compound TGG,C-3
R4697 T8976 T8979 compound GCG,C-3
R4698 T8977 T8979 compound TGA,C-3
R4699 T8978 T8979 compound GTA,C-3
R4700 T8979 T8948 appos C-3,antisense
R4701 T8980 T8979 case ',C-3
R4702 T8981 T8948 punct ;,antisense
R4703 T8982 T8983 compound GAPDH,antisense
R4704 T8983 T8948 appos antisense,antisense
R4705 T8984 T8983 punct ",",antisense
R4706 T8985 T8994 nummod 5,CC-3
R4707 T8986 T8994 punct ',CC-3
R4708 T8987 T8994 punct -,CC-3
R4709 T8988 T8994 compound CGT,CC-3
R4710 T8989 T8994 compound TCA,CC-3
R4711 T8990 T8994 compound GCT,CC-3
R4712 T8991 T8994 compound CAG,CC-3
R4713 T8992 T8994 compound GGA,CC-3
R4714 T8993 T8994 compound TGA,CC-3
R4715 T8994 T8948 appos CC-3,antisense
R4716 T8995 T8994 punct ',CC-3
R4717 T8996 T8929 punct .,used
R4718 T8997 T8999 nsubjpass Reactions,processed
R4719 T8998 T8999 auxpass were,processed
R4720 T8999 T9066 ccomp processed,determined
R4721 T9000 T8999 prep in,processed
R4722 T9001 T9004 det a,cycler
R4723 T9002 T9004 nmod DNA,cycler
R4724 T9003 T9004 amod thermal,cycler
R4725 T9004 T9000 pobj cycler,in
R4726 T9005 T9007 punct (,Cetus
R4727 T9006 T9007 compound Perkin-Elmer,Cetus
R4728 T9007 T9004 appos Cetus,cycler
R4729 T9008 T9007 punct ",",Cetus
R4730 T9009 T9007 npadvmod Norwalk,Cetus
R4731 T9010 T9009 punct ",",Norwalk
R4732 T9011 T9009 conj CT,Norwalk
R4733 T9012 T9011 punct ",",CT
R4734 T9013 T9011 conj USA,CT
R4735 T9014 T9007 punct ),Cetus
R4736 T9015 T8999 prep through,processed
R4737 T9016 T9015 pobj cycles,through
R4738 T9017 T8999 prep for,processed
R4739 T9018 T9017 pobj 30,for
R4740 T9019 T8999 dep s,processed
R4741 T9020 T9019 prep of,s
R4742 T9021 T9020 pobj denaturation,of
R4743 T9022 T8999 prep at,processed
R4744 T9023 T9024 nummod 94,°
R4745 T9024 T9025 nummod °,C
R4746 T9025 T9022 pobj C,at
R4747 T9026 T9025 punct ",",C
R4748 T9027 T9028 nummod 1,min
R4749 T9028 T9040 nsubj min,followed
R4750 T9029 T9028 prep of,min
R4751 T9030 T9029 pcomp annealing,of
R4752 T9031 T9030 prep at,annealing
R4753 T9032 T9034 nummod 56,C
R4754 T9033 T9034 nummod °,C
R4755 T9034 T9031 pobj C,at
R4756 T9035 T9034 prep for,C
R4757 T9036 T9035 pobj GAPDH,for
R4758 T9037 T9036 cc and,GAPDH
R4759 T9038 T9036 conj IL-17,GAPDH
R4760 T9039 T9034 punct ",",C
R4761 T9040 T8999 advcl followed,processed
R4762 T9041 T9040 prep by,followed
R4763 T9042 T9043 nummod 1,min
R4764 T9043 T9041 pobj min,by
R4765 T9044 T9043 prep of,min
R4766 T9045 T9044 pobj elongation,of
R4767 T9046 T9043 prep at,min
R4768 T9047 T9048 nummod 72,°
R4769 T9048 T9051 nummod °,rounds
R4770 T9049 T9050 compound C.,PCR
R4771 T9050 T9051 compound PCR,rounds
R4772 T9051 T9046 pobj rounds,at
R4773 T9052 T9053 auxpass were,repeated
R4774 T9053 T8999 conj repeated,processed
R4775 T9054 T9053 prep for,repeated
R4776 T9055 T9056 nummod 25,cycles
R4777 T9056 T9054 pobj cycles,for
R4778 T9057 T9063 acomp each,;
R4779 T9058 T9057 prep for,each
R4780 T9059 T9060 det both,GAPDH
R4781 T9060 T9058 pobj GAPDH,for
R4782 T9061 T9060 cc and,GAPDH
R4783 T9062 T9060 conj IL-17,GAPDH
R4784 T9063 T9066 punct ;,determined
R4785 T9064 T9066 nsubjpass this,determined
R4786 T9065 T9066 auxpass was,determined
R4787 T9066 T9066 ROOT determined,determined
R4788 T9067 T9066 prep as,determined
R4789 T9068 T9067 pcomp falling,as
R4790 T9069 T9068 prep within,falling
R4791 T9070 T9072 det the,phase
R4792 T9071 T9072 amod exponential,phase
R4793 T9072 T9069 pobj phase,within
R4794 T9073 T9072 prep of,phase
R4795 T9074 T9073 pobj amplification,of
R4796 T9075 T9068 prep for,falling
R4797 T9076 T9077 det each,molecule
R4798 T9077 T9075 pobj molecule,for
R4799 T9078 T9066 punct .,determined
R4800 T9079 T9080 det The,level
R4801 T9080 T9085 nsubjpass level,presented
R4802 T9081 T9080 prep of,level
R4803 T9082 T9083 compound mRNA,expression
R4804 T9083 T9081 pobj expression,of
R4805 T9084 T9085 auxpass was,presented
R4806 T9085 T9085 ROOT presented,presented
R4807 T9086 T9085 prep as,presented
R4808 T9087 T9088 det a,ratio
R4809 T9088 T9086 pobj ratio,as
R4810 T9089 T9088 prep of,ratio
R4811 T9090 T9092 compound IL-17,product
R4812 T9091 T9092 compound PCR,product
R4813 T9092 T9089 pobj product,of
R4814 T9093 T9088 prep over,ratio
R4815 T9094 T9095 compound GAPDH,product
R4816 T9095 T9093 pobj product,over
R4817 T9096 T9085 punct .,presented
R5079 T9686 T9688 amod Western,analysis
R5080 T9687 T9688 compound blot,analysis
R5081 T9688 T9692 nsubj analysis,phosphorylated
R5082 T9689 T9688 prep of,analysis
R5083 T9690 T9689 pobj Akt,of
R5084 T9691 T9688 punct ",",analysis
R5085 T9692 T9692 ROOT phosphorylated,phosphorylated
R5086 T9693 T9692 dobj Akt,phosphorylated
R5087 T9694 T9693 cc and,Akt
R5088 T9695 T9696 compound IκB-α,PBMC
R5089 T9696 T9693 conj PBMC,Akt
R5090 T9697 T9698 auxpass were,incubated
R5091 T9698 T9692 conj incubated,phosphorylated
R5092 T9699 T9698 prep with,incubated
R5093 T9700 T9699 pobj anti-CD3,with
R5094 T9701 T9705 punct (,ml
R5095 T9702 T9703 nummod 10,μg
R5096 T9703 T9705 nmod μg,ml
R5097 T9704 T9705 compound /,ml
R5098 T9705 T9700 appos ml,anti-CD3
R5099 T9706 T9705 punct ),ml
R5100 T9707 T9698 prep in,incubated
R5101 T9708 T9709 det the,presence
R5102 T9709 T9707 pobj presence,in
R5103 T9710 T9709 cc or,presence
R5104 T9711 T9709 conj absence,presence
R5105 T9712 T9711 prep of,absence
R5106 T9713 T9712 pobj LY294002,of
R5107 T9714 T9716 punct (,μM
R5108 T9715 T9716 nummod 20,μM
R5109 T9716 T9713 appos μM,LY294002
R5110 T9717 T9716 punct ),μM
R5111 T9718 T9692 punct .,phosphorylated
R5112 T9719 T9729 prep After,prepared
R5113 T9720 T9719 pobj incubation,After
R5114 T9721 T9729 prep for,prepared
R5115 T9722 T9723 nummod 1,hour
R5116 T9723 T9721 pobj hour,for
R5117 T9724 T9729 punct ",",prepared
R5118 T9725 T9727 amod whole,lysates
R5119 T9726 T9727 compound cell,lysates
R5120 T9727 T9729 nsubjpass lysates,prepared
R5121 T9728 T9729 auxpass were,prepared
R5122 T9729 T9729 ROOT prepared,prepared
R5123 T9730 T9729 prep from,prepared
R5124 T9731 T9733 nmod about,cells
R5125 T9732 T9733 nummod 107,cells
R5126 T9733 T9730 pobj cells,from
R5127 T9734 T9733 prep by,cells
R5128 T9735 T9734 pobj homogenization,by
R5129 T9736 T9733 prep in,cells
R5130 T9737 T9739 det the,buffer
R5131 T9738 T9739 compound lysis,buffer
R5132 T9739 T9736 pobj buffer,in
R5133 T9740 T9729 punct ",",prepared
R5134 T9741 T9729 cc and,prepared
R5135 T9742 T9729 conj centrifuged,prepared
R5136 T9743 T9742 prep at,centrifuged
R5137 T9744 T9745 nummod "14,000",r.p.m.
R5138 T9745 T9743 pobj r.p.m.,at
R5139 T9746 T9745 punct (,r.p.m.
R5140 T9747 T9748 nummod "19,000",g
R5141 T9748 T9745 appos g,r.p.m.
R5142 T9749 T9745 punct ),r.p.m.
R5143 T9750 T9742 prep for,centrifuged
R5144 T9751 T9752 nummod 15,min
R5145 T9752 T9750 pobj min,for
R5146 T9753 T9729 punct .,prepared
R5147 T9754 T9755 compound Protein,concentrations
R5148 T9755 T9760 nsubjpass concentrations,determined
R5149 T9756 T9755 prep in,concentrations
R5150 T9757 T9758 det the,supernatants
R5151 T9758 T9756 pobj supernatants,in
R5152 T9759 T9760 auxpass were,determined
R5153 T9760 T9760 ROOT determined,determined
R5154 T9761 T9760 prep with,determined
R5155 T9762 T9764 det the,method
R5156 T9763 T9764 compound Bradford,method
R5157 T9764 T9761 pobj method,with
R5158 T9765 T9766 punct (,Bio-Rad
R5159 T9766 T9764 appos Bio-Rad,method
R5160 T9767 T9766 punct ",",Bio-Rad
R5161 T9768 T9766 conj Hercules,Bio-Rad
R5162 T9769 T9768 punct ",",Hercules
R5163 T9770 T9768 conj CA,Hercules
R5164 T9771 T9770 punct ",",CA
R5165 T9772 T9770 conj USA,CA
R5166 T9773 T9766 punct ),Bio-Rad
R5167 T9774 T9760 punct .,determined
R5168 T9775 T9776 compound Protein,samples
R5169 T9776 T9778 nsubjpass samples,separated
R5170 T9777 T9778 auxpass were,separated
R5171 T9778 T9778 ROOT separated,separated
R5172 T9779 T9778 prep by,separated
R5173 T9780 T9781 nummod 10,%
R5174 T9781 T9779 pobj %,by
R5175 T9782 T9783 compound SDS,PAGE
R5176 T9783 T9778 npadvmod PAGE,separated
R5177 T9784 T9778 cc and,separated
R5178 T9785 T9778 conj transferred,separated
R5179 T9786 T9785 prep to,transferred
R5180 T9787 T9789 det a,membrane
R5181 T9788 T9789 amod nitrocellulose,membrane
R5182 T9789 T9786 pobj membrane,to
R5183 T9790 T9793 punct (,Biotech
R5184 T9791 T9793 compound Amersham,Biotech
R5185 T9792 T9793 compound Pharmacia,Biotech
R5186 T9793 T9789 appos Biotech,membrane
R5187 T9794 T9793 punct ",",Biotech
R5188 T9795 T9793 conj Uppsala,Biotech
R5189 T9796 T9795 punct ",",Uppsala
R5190 T9797 T9795 conj Sweden,Uppsala
R5191 T9798 T9793 punct ),Biotech
R5192 T9799 T9778 punct .,separated
R5193 T9800 T9806 prep For,preincubated
R5194 T9801 T9802 amod western,hybridization
R5195 T9802 T9800 pobj hybridization,For
R5196 T9803 T9806 punct ",",preincubated
R5197 T9804 T9806 nsubjpass membrane,preincubated
R5198 T9805 T9806 auxpass was,preincubated
R5199 T9806 T9806 ROOT preincubated,preincubated
R5200 T9807 T9806 prep with,preincubated
R5201 T9808 T9809 nummod 0.1,%
R5202 T9809 T9810 nsubj %,skimmed
R5203 T9810 T9806 conj skimmed,preincubated
R5204 T9811 T9810 dobj milk,skimmed
R5205 T9812 T9810 prep in,skimmed
R5206 T9813 T9814 compound TBS-T,buffer
R5207 T9814 T9812 pobj buffer,in
R5208 T9815 T9817 punct (,%
R5209 T9816 T9817 nummod 0.1,%
R5210 T9817 T9837 nsubj %,phosphorylated
R5211 T9818 T9819 nummod Tween,20
R5212 T9819 T9837 dep 20,phosphorylated
R5213 T9820 T9819 prep in,20
R5214 T9821 T9822 amod Tris-buffered,saline
R5215 T9822 T9820 pobj saline,in
R5216 T9823 T9819 punct ),20
R5217 T9824 T9819 prep at,20
R5218 T9825 T9826 compound room,temperature
R5219 T9826 T9824 pobj temperature,at
R5220 T9827 T9819 prep for,20
R5221 T9828 T9829 nummod 2,hours
R5222 T9829 T9827 pobj hours,for
R5223 T9830 T9819 punct ",",20
R5224 T9831 T9833 advmod then,antibodies
R5225 T9832 T9833 amod primary,antibodies
R5226 T9833 T9819 appos antibodies,20
R5227 T9834 T9833 prep against,antibodies
R5228 T9835 T9834 pobj Akt,against
R5229 T9836 T9819 punct ",",20
R5230 T9837 T9810 advcl phosphorylated,skimmed
R5231 T9838 T9837 dobj Akt,phosphorylated
R5232 T9839 T9838 cc and,Akt
R5233 T9840 T9838 conj IκB-α,Akt
R5234 T9841 T9845 punct (,Inc.
R5235 T9842 T9845 compound Cell,Inc.
R5236 T9843 T9845 compound Signaling,Inc.
R5237 T9844 T9845 compound Technology,Inc.
R5238 T9845 T9838 conj Inc.,Akt
R5239 T9846 T9845 punct ",",Inc.
R5240 T9847 T9845 npadvmod Beverly,Inc.
R5241 T9848 T9847 punct ",",Beverly
R5242 T9849 T9847 conj MA,Beverly
R5243 T9850 T9849 punct ",",MA
R5244 T9851 T9849 npadvmod USA,MA
R5245 T9852 T9849 punct ),MA
R5246 T9853 T9845 punct ",",Inc.
R5247 T9854 T9837 prep diluted,phosphorylated
R5248 T9855 T9854 npadvmod 1:1000,diluted
R5249 T9856 T9855 prep in,1:1000
R5250 T9857 T9858 nummod 5,%
R5251 T9858 T9859 compound %,BSA/TBS-T
R5252 T9859 T9856 pobj BSA/TBS-T,in
R5253 T9860 T9862 punct ",",added
R5254 T9861 T9862 auxpass were,added
R5255 T9862 T9806 conj added,preincubated
R5256 T9863 T9862 cc and,added
R5257 T9864 T9862 conj incubated,added
R5258 T9865 T9864 advmod overnight,incubated
R5259 T9866 T9864 prep at,incubated
R5260 T9867 T9868 nummod 4,°
R5261 T9868 T9866 pobj °,at
R5262 T9869 T9862 nsubj C,added
R5263 T9870 T9862 punct .,added
R5264 T9871 T9883 prep After,added
R5265 T9872 T9871 pcomp washing,After
R5266 T9873 T9874 nummod four,times
R5267 T9874 T9872 dobj times,washing
R5268 T9875 T9872 prep with,washing
R5269 T9876 T9875 pobj TBS-T,with
R5270 T9877 T9883 punct ",",added
R5271 T9878 T9881 amod horseradish,antibodies
R5272 T9879 T9881 amod peroxidase-conjugated,antibodies
R5273 T9880 T9881 amod secondary,antibodies
R5274 T9881 T9883 nsubjpass antibodies,added
R5275 T9882 T9883 auxpass were,added
R5276 T9883 T9883 ROOT added,added
R5277 T9884 T9883 cc and,added
R5278 T9885 T9883 conj allowed,added
R5279 T9886 T9887 aux to,incubate
R5280 T9887 T9885 xcomp incubate,allowed
R5281 T9888 T9887 prep for,incubate
R5282 T9889 T9890 nummod 1,hour
R5283 T9890 T9888 pobj hour,for
R5284 T9891 T9887 prep at,incubate
R5285 T9892 T9893 compound room,temperature
R5286 T9893 T9891 pobj temperature,at
R5287 T9894 T9883 punct .,added
R5288 T9895 T9902 prep After,detected
R5289 T9896 T9897 nsubj TBS-T,washing
R5290 T9897 T9895 pcomp washing,After
R5291 T9898 T9902 punct ",",detected
R5292 T9899 T9900 amod hybridized,bands
R5293 T9900 T9902 nsubjpass bands,detected
R5294 T9901 T9902 auxpass were,detected
R5295 T9902 T9902 ROOT detected,detected
R5296 T9903 T9902 prep with,detected
R5297 T9904 T9906 det the,chemiluminescence
R5298 T9905 T9906 amod enhanced,chemiluminescence
R5299 T9906 T9903 pobj chemiluminescence,with
R5300 T9907 T9906 punct (,chemiluminescence
R5301 T9908 T9906 appos ECL,chemiluminescence
R5302 T9909 T9911 punct ),kit
R5303 T9910 T9911 compound detection,kit
R5304 T9911 T9906 conj kit,chemiluminescence
R5305 T9912 T9911 cc and,kit
R5306 T9913 T9914 amod Hyperfilm-ECL,reagents
R5307 T9914 T9911 conj reagents,kit
R5308 T9915 T9917 punct (,Pharmacia
R5309 T9916 T9917 compound Amersham,Pharmacia
R5310 T9917 T9914 appos Pharmacia,reagents
R5311 T9918 T9914 punct ),reagents
R5312 T9919 T9902 punct .,detected
R5544 T10457 T10459 nmod Gel,assay
R5545 T10458 T10459 amod mobility-shift,assay
R5546 T10459 T10467 nsubjpass assay,extracted
R5547 T10460 T10459 prep of,assay
R5548 T10461 T10463 amod NF-κB,site
R5549 T10462 T10463 amod binding,site
R5550 T10463 T10465 compound site,proteins
R5551 T10464 T10465 compound Nuclear,proteins
R5552 T10465 T10460 pobj proteins,of
R5553 T10466 T10467 auxpass were,extracted
R5554 T10467 T10467 ROOT extracted,extracted
R5555 T10468 T10467 prep from,extracted
R5556 T10469 T10473 nmod about,PBMC
R5557 T10470 T10473 nummod 5,PBMC
R5558 T10471 T10473 nummod ×,PBMC
R5559 T10472 T10473 nummod 106,PBMC
R5560 T10473 T10468 pobj PBMC,from
R5561 T10474 T10467 punct .,extracted
R5562 T10475 T10476 compound Oligonucleotide,probes
R5563 T10476 T10502 nsubjpass probes,generated
R5564 T10477 T10476 acl encompassing,probes
R5565 T10478 T10481 det the,site
R5566 T10479 T10481 amod NF-κB,site
R5567 T10480 T10481 amod binding,site
R5568 T10481 T10477 dobj site,encompassing
R5569 T10482 T10481 prep of,site
R5570 T10483 T10486 det the,promoter
R5571 T10484 T10486 amod human,promoter
R5572 T10485 T10486 compound IL-17,promoter
R5573 T10486 T10482 pobj promoter,of
R5574 T10487 T10486 punct (,promoter
R5575 T10488 T10486 appos 5,promoter
R5576 T10489 T10486 punct ',promoter
R5577 T10490 T10502 punct -,generated
R5578 T10491 T10502 nsubjpass ATG,generated
R5579 T10492 T10502 nsubjpass ACC,generated
R5580 T10493 T10495 compound TGG,TAC
R5581 T10494 T10495 compound AAA,TAC
R5582 T10495 T10502 nsubjpass TAC,generated
R5583 T10496 T10497 compound CCA,AAA
R5584 T10497 T10498 compound AAA,TTC-3
R5585 T10498 T10495 appos TTC-3,TAC
R5586 T10499 T10495 punct ',TAC
R5587 T10500 T10495 punct ),TAC
R5588 T10501 T10502 auxpass were,generated
R5589 T10502 T10502 ROOT generated,generated
R5590 T10503 T10502 agent by,generated
R5591 T10504 T10503 pobj 5,by
R5592 T10505 T10504 punct ',5
R5593 T10506 T10507 punct -,end
R5594 T10507 T10508 compound end,labeling
R5595 T10508 T10502 dobj labeling,generated
R5596 T10509 T10508 prep of,labeling
R5597 T10510 T10512 det the,strand
R5598 T10511 T10512 compound sense,strand
R5599 T10512 T10509 pobj strand,of
R5600 T10513 T10512 prep with,strand
R5601 T10514 T10517 compound [,dATP
R5602 T10515 T10517 compound γ-32P,dATP
R5603 T10516 T10517 compound ],dATP
R5604 T10517 T10513 pobj dATP,with
R5605 T10518 T10520 punct (,Pharmacia
R5606 T10519 T10520 compound Amersham,Pharmacia
R5607 T10520 T10517 appos Pharmacia,dATP
R5608 T10521 T10520 punct ),Pharmacia
R5609 T10522 T10517 cc and,dATP
R5610 T10523 T10525 nummod T4,kinase
R5611 T10524 T10525 compound polynucleotide,kinase
R5612 T10525 T10517 conj kinase,dATP
R5613 T10526 T10525 punct (,kinase
R5614 T10527 T10525 appos TaKaRa,kinase
R5615 T10528 T10525 punct ),kinase
R5616 T10529 T10502 punct .,generated
R5617 T10530 T10531 compound Unincorporated,nucleotides
R5618 T10531 T10533 nsubjpass nucleotides,removed
R5619 T10532 T10533 auxpass were,removed
R5620 T10533 T10533 ROOT removed,removed
R5621 T10534 T10533 agent by,removed
R5622 T10535 T10538 nmod NucTrap,columns
R5623 T10536 T10537 compound probe,purification
R5624 T10537 T10538 compound purification,columns
R5625 T10538 T10534 pobj columns,by
R5626 T10539 T10538 punct (,columns
R5627 T10540 T10538 appos Stratagene,columns
R5628 T10541 T10540 punct ",",Stratagene
R5629 T10542 T10543 compound La,Jolla
R5630 T10543 T10540 conj Jolla,Stratagene
R5631 T10544 T10543 punct ",",Jolla
R5632 T10545 T10543 conj CA,Jolla
R5633 T10546 T10545 punct ",",CA
R5634 T10547 T10545 conj USA,CA
R5635 T10548 T10538 punct ),columns
R5636 T10549 T10533 punct .,removed
R5637 T10550 T10551 compound Nuclear,extracts
R5638 T10551 T10559 nsubjpass extracts,incubated
R5639 T10552 T10554 punct (,μg
R5640 T10553 T10554 nummod 2,μg
R5641 T10554 T10551 appos μg,extracts
R5642 T10555 T10554 prep of,μg
R5643 T10556 T10555 pobj protein,of
R5644 T10557 T10554 punct ),μg
R5645 T10558 T10559 auxpass were,incubated
R5646 T10559 T10559 ROOT incubated,incubated
R5647 T10560 T10559 prep with,incubated
R5648 T10561 T10563 amod radiolabeled,probes
R5649 T10562 T10563 compound DNA,probes
R5650 T10563 T10560 pobj probes,with
R5651 T10564 T10566 punct (,ng
R5652 T10565 T10566 nummod 10,ng
R5653 T10566 T10563 appos ng,probes
R5654 T10567 T10566 punct ;,ng
R5655 T10568 T10569 nummod "100,000",c.p.m.
R5656 T10569 T10566 appos c.p.m.,ng
R5657 T10570 T10566 punct ),ng
R5658 T10571 T10566 prep for,ng
R5659 T10572 T10573 nummod 30,min
R5660 T10573 T10571 pobj min,for
R5661 T10574 T10573 prep at,min
R5662 T10575 T10576 compound room,temperature
R5663 T10576 T10574 pobj temperature,at
R5664 T10577 T10573 prep in,min
R5665 T10578 T10579 nummod 20,μl
R5666 T10579 T10577 pobj μl,in
R5667 T10580 T10579 prep of,μl
R5668 T10581 T10582 amod binding,buffer
R5669 T10582 T10580 pobj buffer,of
R5670 T10583 T10582 acl consisting,buffer
R5671 T10584 T10583 prep of,consisting
R5672 T10585 T10587 nummod 20,Tris-HCl
R5673 T10586 T10587 compound mM,Tris-HCl
R5674 T10587 T10584 pobj Tris-HCl,of
R5675 T10588 T10587 punct ",",Tris-HCl
R5676 T10589 T10587 appos pH,Tris-HCl
R5677 T10590 T10589 nummod 7.9,pH
R5678 T10591 T10589 punct ",",pH
R5679 T10592 T10594 nummod 50,KCl
R5680 T10593 T10594 compound mM,KCl
R5681 T10594 T10587 appos KCl,Tris-HCl
R5682 T10595 T10594 punct ",",KCl
R5683 T10596 T10598 nummod 1,dithiothreitol
R5684 T10597 T10598 compound mM,dithiothreitol
R5685 T10598 T10594 appos dithiothreitol,KCl
R5686 T10599 T10598 punct ",",dithiothreitol
R5687 T10600 T10602 nummod 0.5,EDTA
R5688 T10601 T10602 compound mM,EDTA
R5689 T10602 T10598 appos EDTA,dithiothreitol
R5690 T10603 T10602 punct ",",EDTA
R5691 T10604 T10605 nummod 5,%
R5692 T10605 T10606 compound %,glycerol
R5693 T10606 T10602 appos glycerol,EDTA
R5694 T10607 T10606 punct ",",glycerol
R5695 T10608 T10609 nummod 1,mg/ml
R5696 T10609 T10610 compound mg/ml,BSA
R5697 T10610 T10602 appos BSA,EDTA
R5698 T10611 T10602 punct ",",EDTA
R5699 T10612 T10613 nummod 0.2,%
R5700 T10613 T10615 compound %,P40
R5701 T10614 T10615 compound Nonidet,P40
R5702 T10615 T10602 conj P40,EDTA
R5703 T10616 T10615 cc and,P40
R5704 T10617 T10618 nummod 50,ng
R5705 T10618 T10621 compound ng,poly
R5706 T10619 T10621 compound /,poly
R5707 T10620 T10621 compound μl,poly
R5708 T10621 T10615 conj poly,P40
R5709 T10622 T10623 punct (,dI-dC
R5710 T10623 T10621 parataxis dI-dC,poly
R5711 T10624 T10623 punct ),dI-dC
R5712 T10625 T10559 punct .,incubated
R5713 T10626 T10628 nsubjpass Samples,subjected
R5714 T10627 T10628 auxpass were,subjected
R5715 T10628 T10628 ROOT subjected,subjected
R5716 T10629 T10628 prep to,subjected
R5717 T10630 T10629 pobj electrophoresis,to
R5718 T10631 T10630 prep on,electrophoresis
R5719 T10632 T10631 pcomp nondenaturing,on
R5720 T10633 T10634 nummod 5,%
R5721 T10634 T10636 compound %,gels
R5722 T10635 T10636 compound polyacrylamide,gels
R5723 T10636 T10632 dobj gels,nondenaturing
R5724 T10637 T10632 prep in,nondenaturing
R5725 T10638 T10641 nummod 0.5,buffer
R5726 T10639 T10641 nummod ×,buffer
R5727 T10640 T10641 compound Tris-borate-EDTA,buffer
R5728 T10641 T10637 pobj buffer,in
R5729 T10642 T10643 punct (,pH
R5730 T10643 T10629 pobj pH,to
R5731 T10644 T10643 nummod 8.0,pH
R5732 T10645 T10643 punct ),pH
R5733 T10646 T10628 prep at,subjected
R5734 T10647 T10649 nummod 100,Gels
R5735 T10648 T10649 compound V.,Gels
R5736 T10649 T10646 pobj Gels,at
R5737 T10650 T10651 auxpass were,dried
R5738 T10651 T10628 conj dried,subjected
R5739 T10652 T10651 prep under,dried
R5740 T10653 T10652 pobj vacuum,under
R5741 T10654 T10651 cc and,dried
R5742 T10655 T10651 conj exposed,dried
R5743 T10656 T10655 prep to,exposed
R5744 T10657 T10659 compound Kodak,film
R5745 T10658 T10659 compound X-OMAT,film
R5746 T10659 T10656 pobj film,to
R5747 T10660 T10655 prep at,exposed
R5748 T10661 T10662 nummod -70,°
R5749 T10662 T10660 pobj °,at
R5750 T10663 T10655 npadvmod C,exposed
R5751 T10664 T10655 prep with,exposed
R5752 T10665 T10666 amod intensifying,screens
R5753 T10666 T10664 pobj screens,with
R5754 T10667 T10628 punct .,subjected
R5755 T10668 T10670 compound Rabbit,antibodies
R5756 T10669 T10670 compound polyclonal,antibodies
R5757 T10670 T10673 nsubj antibodies,subunits
R5758 T10671 T10670 prep against,antibodies
R5759 T10672 T10671 pobj NF-κB,against
R5760 T10673 T10673 ROOT subunits,subunits
R5761 T10674 T10673 dobj p50,subunits
R5762 T10675 T10674 punct ",",p50
R5763 T10676 T10674 conj p65,p50
R5764 T10677 T10679 cc and,were
R5765 T10678 T10679 nsubj c-Rel,were
R5766 T10679 T10679 ROOT were,were
R5767 T10680 T10679 prep from,were
R5768 T10681 T10683 compound Santa,Biotechnology
R5769 T10682 T10683 compound Cruz,Biotechnology
R5770 T10683 T10680 pobj Biotechnology,from
R5771 T10684 T10683 punct (,Biotechnology
R5772 T10685 T10686 compound Santa,Cruz
R5773 T10686 T10683 appos Cruz,Biotechnology
R5774 T10687 T10683 punct ",",Biotechnology
R5775 T10688 T10683 conj CA,Biotechnology
R5776 T10689 T10688 punct ",",CA
R5777 T10690 T10688 conj USA,CA
R5778 T10691 T10679 punct ),were
R5779 T10692 T10679 punct .,were
R5856 T10901 T10902 compound Cell,viability
R5857 T10902 T10902 ROOT viability,viability
R5858 T10903 T10908 punct (,assay
R5859 T10904 T10908 nmod Trypan,assay
R5860 T10905 T10906 amod blue,dye
R5861 T10906 T10908 nmod dye,assay
R5862 T10907 T10908 compound exclusion,assay
R5863 T10908 T10902 appos assay,viability
R5864 T10909 T10902 punct ),viability
R5865 T10910 T10922 prep For,used
R5866 T10911 T10912 compound cell,viability
R5867 T10912 T10913 compound viability,assays
R5868 T10913 T10910 pobj assays,For
R5869 T10914 T10922 punct ",",used
R5870 T10915 T10920 det the,method
R5871 T10916 T10920 amod trypan,method
R5872 T10917 T10918 amod blue,dye
R5873 T10918 T10919 compound dye,exclusion
R5874 T10919 T10920 compound exclusion,method
R5875 T10920 T10922 nsubjpass method,used
R5876 T10921 T10922 auxpass was,used
R5877 T10922 T10922 ROOT used,used
R5878 T10923 T10924 aux to,evaluate
R5879 T10924 T10922 xcomp evaluate,used
R5880 T10925 T10926 det the,potential
R5881 T10926 T10924 dobj potential,evaluate
R5882 T10927 T10926 prep of,potential
R5883 T10928 T10930 amod direct,effect
R5884 T10929 T10930 amod cytotoxic,effect
R5885 T10930 T10927 pobj effect,of
R5886 T10931 T10930 prep of,effect
R5887 T10932 T10931 pobj inhibitors,of
R5888 T10933 T10932 prep on,inhibitors
R5889 T10934 T10933 pobj cells,on
R5890 T10935 T10922 punct .,used
R5891 T10936 T10945 prep After,harvested
R5892 T10937 T10936 pobj incubation,After
R5893 T10938 T10937 prep for,incubation
R5894 T10939 T10940 nummod 24,hours
R5895 T10940 T10938 pobj hours,for
R5896 T10941 T10945 punct ",",harvested
R5897 T10942 T10943 det the,cells
R5898 T10943 T10945 nsubjpass cells,harvested
R5899 T10944 T10945 auxpass were,harvested
R5900 T10945 T10945 ROOT harvested,harvested
R5901 T10946 T10945 cc and,harvested
R5902 T10947 T10950 det the,viability
R5903 T10948 T10949 compound percentage,cell
R5904 T10949 T10950 compound cell,viability
R5905 T10950 T10952 nsubjpass viability,calculated
R5906 T10951 T10952 auxpass was,calculated
R5907 T10952 T10945 conj calculated,harvested
R5908 T10953 T10952 prep with,calculated
R5909 T10954 T10955 det the,formula
R5910 T10955 T10953 pobj formula,with
R5911 T10956 T10957 nummod 100,×
R5912 T10957 T10972 npadvmod ×,]
R5913 T10958 T10959 punct (,number
R5914 T10959 T10972 nmod number,]
R5915 T10960 T10959 prep of,number
R5916 T10961 T10962 amod viable,cells/number
R5917 T10962 T10960 pobj cells/number,of
R5918 T10963 T10962 prep of,cells/number
R5919 T10964 T10965 preconj both,viable
R5920 T10965 T10968 amod viable,cells
R5921 T10966 T10965 cc and,viable
R5922 T10967 T10965 conj dead,viable
R5923 T10968 T10963 pobj cells,of
R5924 T10969 T10959 punct ),number
R5925 T10970 T10972 quantmod [,]
R5926 T10971 T10972 nummod 21,]
R5927 T10972 T10952 conj ],calculated
R5928 T10973 T10952 punct .,calculated
R5957 T11046 T11047 compound Statistical,analysis
R5958 T11047 T11048 compound analysis,Data
R5959 T11048 T11050 nsubjpass Data,expressed
R5960 T11049 T11050 auxpass are,expressed
R5961 T11050 T11050 ROOT expressed,expressed
R5962 T11051 T11052 mark as,means
R5963 T11052 T11050 advcl means,expressed
R5964 T11053 T11054 compound ±,SEM
R5965 T11054 T11052 dobj SEM,means
R5966 T11055 T11054 punct .,SEM
R5967 T11056 T11057 compound Statistical,analysis
R5968 T11057 T11059 nsubjpass analysis,performed
R5969 T11058 T11059 auxpass was,performed
R5970 T11059 T11059 ROOT performed,performed
R5971 T11060 T11059 prep with,performed
R5972 T11061 T11060 pobj Student,with
R5973 T11062 T11061 case 's,Student
R5974 T11063 T11061 amod t-test,Student
R5975 T11064 T11063 prep for,t-test
R5976 T11065 T11066 amod matched,pairs
R5977 T11066 T11064 pobj pairs,for
R5978 T11067 T11059 punct .,performed
R5979 T11068 T11069 nsubj P,values
R5980 T11069 T11069 ROOT values,values
R5981 T11070 T11072 amod less,0.05
R5982 T11071 T11072 quantmod than,0.05
R5983 T11072 T11074 nsubjpass 0.05,considered
R5984 T11073 T11074 auxpass were,considered
R5985 T11074 T11069 ccomp considered,values
R5986 T11075 T11074 oprd significant,considered
R5987 T11076 T11069 punct .,values