| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T9686 |
0-7 |
JJ |
denotes |
Western |
| T9687 |
8-12 |
NN |
denotes |
blot |
| T9688 |
13-21 |
NN |
denotes |
analysis |
| T9689 |
22-24 |
IN |
denotes |
of |
| T9690 |
25-28 |
NNP |
denotes |
Akt |
| T9691 |
28-29 |
, |
denotes |
, |
| T9692 |
30-44 |
VBD |
denotes |
phosphorylated |
| T9693 |
45-48 |
NNP |
denotes |
Akt |
| T9694 |
49-52 |
CC |
denotes |
and |
| T9695 |
53-58 |
NNP |
denotes |
IκB-α |
| T9696 |
59-63 |
NNP |
denotes |
PBMC |
| T9697 |
64-68 |
VBD |
denotes |
were |
| T9698 |
69-78 |
VBN |
denotes |
incubated |
| T9699 |
79-83 |
IN |
denotes |
with |
| T9700 |
84-92 |
JJ |
denotes |
anti-CD3 |
| T9701 |
93-94 |
-LRB- |
denotes |
( |
| T9702 |
94-96 |
CD |
denotes |
10 |
| T9703 |
97-99 |
NN |
denotes |
μg |
| T9704 |
99-100 |
NN |
denotes |
/ |
| T9705 |
100-102 |
NN |
denotes |
ml |
| T9706 |
102-103 |
-RRB- |
denotes |
) |
| T9707 |
104-106 |
IN |
denotes |
in |
| T9708 |
107-110 |
DT |
denotes |
the |
| T9709 |
111-119 |
NN |
denotes |
presence |
| T9710 |
120-122 |
CC |
denotes |
or |
| T9711 |
123-130 |
NN |
denotes |
absence |
| T9712 |
131-133 |
IN |
denotes |
of |
| T9713 |
134-142 |
NNP |
denotes |
LY294002 |
| T9714 |
143-144 |
-LRB- |
denotes |
( |
| T9715 |
144-146 |
CD |
denotes |
20 |
| T9716 |
147-149 |
NNP |
denotes |
μM |
| T9717 |
149-150 |
-RRB- |
denotes |
) |
| T9718 |
150-151 |
. |
denotes |
. |
| T9719 |
152-157 |
IN |
denotes |
After |
| T9720 |
158-168 |
NN |
denotes |
incubation |
| T9721 |
169-172 |
IN |
denotes |
for |
| T9722 |
173-174 |
CD |
denotes |
1 |
| T9723 |
175-179 |
NN |
denotes |
hour |
| T9724 |
179-180 |
, |
denotes |
, |
| T9725 |
181-186 |
JJ |
denotes |
whole |
| T9726 |
187-191 |
NN |
denotes |
cell |
| T9727 |
192-199 |
NNS |
denotes |
lysates |
| T9728 |
200-204 |
VBD |
denotes |
were |
| T9729 |
205-213 |
VBN |
denotes |
prepared |
| T9730 |
214-218 |
IN |
denotes |
from |
| T9731 |
219-224 |
IN |
denotes |
about |
| T9732 |
225-228 |
CD |
denotes |
107 |
| T9733 |
229-234 |
NNS |
denotes |
cells |
| T9734 |
235-237 |
IN |
denotes |
by |
| T9735 |
238-252 |
NN |
denotes |
homogenization |
| T9736 |
253-255 |
IN |
denotes |
in |
| T9737 |
256-259 |
DT |
denotes |
the |
| T9738 |
260-265 |
NN |
denotes |
lysis |
| T9739 |
266-272 |
NN |
denotes |
buffer |
| T9740 |
272-273 |
, |
denotes |
, |
| T9741 |
274-277 |
CC |
denotes |
and |
| T9742 |
278-289 |
VBD |
denotes |
centrifuged |
| T9743 |
290-292 |
IN |
denotes |
at |
| T9744 |
293-299 |
CD |
denotes |
14,000 |
| T9745 |
300-306 |
NN |
denotes |
r.p.m. |
| T9746 |
307-308 |
-LRB- |
denotes |
( |
| T9747 |
308-314 |
CD |
denotes |
19,000 |
| T9748 |
315-316 |
NN |
denotes |
g |
| T9749 |
316-317 |
-RRB- |
denotes |
) |
| T9750 |
318-321 |
IN |
denotes |
for |
| T9751 |
322-324 |
CD |
denotes |
15 |
| T9752 |
325-328 |
NN |
denotes |
min |
| T9753 |
328-329 |
. |
denotes |
. |
| T9754 |
330-337 |
NN |
denotes |
Protein |
| T9755 |
338-352 |
NNS |
denotes |
concentrations |
| T9756 |
353-355 |
IN |
denotes |
in |
| T9757 |
356-359 |
DT |
denotes |
the |
| T9758 |
360-372 |
NNS |
denotes |
supernatants |
| T9759 |
373-377 |
VBD |
denotes |
were |
| T9760 |
378-388 |
VBN |
denotes |
determined |
| T9761 |
389-393 |
IN |
denotes |
with |
| T9762 |
394-397 |
DT |
denotes |
the |
| T9763 |
398-406 |
NNP |
denotes |
Bradford |
| T9764 |
407-413 |
NN |
denotes |
method |
| T9765 |
414-415 |
-LRB- |
denotes |
( |
| T9766 |
415-422 |
NNP |
denotes |
Bio-Rad |
| T9767 |
422-423 |
, |
denotes |
, |
| T9768 |
424-432 |
NNP |
denotes |
Hercules |
| T9769 |
432-433 |
, |
denotes |
, |
| T9770 |
434-436 |
NNP |
denotes |
CA |
| T9771 |
436-437 |
, |
denotes |
, |
| T9772 |
438-441 |
NNP |
denotes |
USA |
| T9773 |
441-442 |
-RRB- |
denotes |
) |
| T9774 |
442-443 |
. |
denotes |
. |
| T9775 |
444-451 |
NN |
denotes |
Protein |
| T9776 |
452-459 |
NNS |
denotes |
samples |
| T9777 |
460-464 |
VBD |
denotes |
were |
| T9778 |
465-474 |
VBN |
denotes |
separated |
| T9779 |
475-477 |
IN |
denotes |
by |
| T9780 |
478-480 |
CD |
denotes |
10 |
| T9781 |
480-481 |
NN |
denotes |
% |
| T9782 |
482-485 |
NNP |
denotes |
SDS |
| T9783 |
486-490 |
NNP |
denotes |
PAGE |
| T9784 |
491-494 |
CC |
denotes |
and |
| T9785 |
495-506 |
VBN |
denotes |
transferred |
| T9786 |
507-509 |
TO |
denotes |
to |
| T9787 |
510-511 |
DT |
denotes |
a |
| T9788 |
512-526 |
JJ |
denotes |
nitrocellulose |
| T9789 |
527-535 |
NN |
denotes |
membrane |
| T9790 |
536-537 |
-LRB- |
denotes |
( |
| T9791 |
537-545 |
NNP |
denotes |
Amersham |
| T9792 |
546-555 |
NNP |
denotes |
Pharmacia |
| T9793 |
556-563 |
NNP |
denotes |
Biotech |
| T9794 |
563-564 |
, |
denotes |
, |
| T9795 |
565-572 |
NNP |
denotes |
Uppsala |
| T9796 |
572-573 |
, |
denotes |
, |
| T9797 |
574-580 |
NNP |
denotes |
Sweden |
| T9798 |
580-581 |
-RRB- |
denotes |
) |
| T9799 |
581-582 |
. |
denotes |
. |
| T9800 |
583-586 |
IN |
denotes |
For |
| T9801 |
587-594 |
JJ |
denotes |
western |
| T9802 |
595-608 |
NN |
denotes |
hybridization |
| T9803 |
608-609 |
, |
denotes |
, |
| T9804 |
610-618 |
NN |
denotes |
membrane |
| T9805 |
619-622 |
VBD |
denotes |
was |
| T9806 |
623-635 |
VBN |
denotes |
preincubated |
| T9807 |
636-640 |
IN |
denotes |
with |
| T9808 |
641-644 |
CD |
denotes |
0.1 |
| T9809 |
644-645 |
NN |
denotes |
% |
| T9810 |
646-653 |
VBD |
denotes |
skimmed |
| T9811 |
654-658 |
NN |
denotes |
milk |
| T9812 |
659-661 |
IN |
denotes |
in |
| T9813 |
662-667 |
NNP |
denotes |
TBS-T |
| T9814 |
668-674 |
NN |
denotes |
buffer |
| T9815 |
675-676 |
-LRB- |
denotes |
( |
| T9816 |
676-679 |
CD |
denotes |
0.1 |
| T9817 |
679-680 |
NN |
denotes |
% |
| T9818 |
681-686 |
CD |
denotes |
Tween |
| T9819 |
687-689 |
CD |
denotes |
20 |
| T9820 |
690-692 |
IN |
denotes |
in |
| T9821 |
693-706 |
JJ |
denotes |
Tris-buffered |
| T9822 |
707-713 |
NN |
denotes |
saline |
| T9823 |
713-714 |
-RRB- |
denotes |
) |
| T9824 |
715-717 |
IN |
denotes |
at |
| T9825 |
718-722 |
NN |
denotes |
room |
| T9826 |
723-734 |
NN |
denotes |
temperature |
| T9827 |
735-738 |
IN |
denotes |
for |
| T9828 |
739-740 |
CD |
denotes |
2 |
| T9829 |
741-746 |
NNS |
denotes |
hours |
| T9830 |
746-747 |
, |
denotes |
, |
| T9831 |
748-752 |
RB |
denotes |
then |
| T9832 |
753-760 |
JJ |
denotes |
primary |
| T9833 |
761-771 |
NNS |
denotes |
antibodies |
| T9834 |
772-779 |
IN |
denotes |
against |
| T9835 |
780-783 |
NNP |
denotes |
Akt |
| T9836 |
783-784 |
, |
denotes |
, |
| T9837 |
785-799 |
VBD |
denotes |
phosphorylated |
| T9838 |
800-803 |
NNP |
denotes |
Akt |
| T9839 |
804-807 |
CC |
denotes |
and |
| T9840 |
808-813 |
NNP |
denotes |
IκB-α |
| T9841 |
814-815 |
-LRB- |
denotes |
( |
| T9842 |
815-819 |
NNP |
denotes |
Cell |
| T9843 |
820-829 |
NNP |
denotes |
Signaling |
| T9844 |
830-840 |
NNP |
denotes |
Technology |
| T9845 |
841-845 |
NNP |
denotes |
Inc. |
| T9846 |
845-846 |
, |
denotes |
, |
| T9847 |
847-854 |
NNP |
denotes |
Beverly |
| T9848 |
854-855 |
, |
denotes |
, |
| T9849 |
856-858 |
NNP |
denotes |
MA |
| T9850 |
858-859 |
, |
denotes |
, |
| T9851 |
860-863 |
NNP |
denotes |
USA |
| T9852 |
863-864 |
-RRB- |
denotes |
) |
| T9853 |
864-865 |
, |
denotes |
, |
| T9854 |
866-873 |
VBN |
denotes |
diluted |
| T9855 |
874-880 |
CD |
denotes |
1:1000 |
| T9856 |
881-883 |
IN |
denotes |
in |
| T9857 |
884-885 |
CD |
denotes |
5 |
| T9858 |
885-886 |
NN |
denotes |
% |
| T9859 |
887-896 |
NN |
denotes |
BSA/TBS-T |
| T9860 |
896-897 |
, |
denotes |
, |
| T9861 |
898-902 |
VBD |
denotes |
were |
| T9862 |
903-908 |
VBN |
denotes |
added |
| T9863 |
909-912 |
CC |
denotes |
and |
| T9864 |
913-922 |
VBN |
denotes |
incubated |
| T9865 |
923-932 |
JJ |
denotes |
overnight |
| T9866 |
933-935 |
IN |
denotes |
at |
| T9867 |
936-937 |
CD |
denotes |
4 |
| T9868 |
937-938 |
CD |
denotes |
° |
| T9869 |
938-939 |
NNP |
denotes |
C |
| T9870 |
939-940 |
. |
denotes |
. |
| T9871 |
941-946 |
IN |
denotes |
After |
| T9872 |
947-954 |
VBG |
denotes |
washing |
| T9873 |
955-959 |
CD |
denotes |
four |
| T9874 |
960-965 |
NNS |
denotes |
times |
| T9875 |
966-970 |
IN |
denotes |
with |
| T9876 |
971-976 |
NNP |
denotes |
TBS-T |
| T9877 |
976-977 |
, |
denotes |
, |
| T9878 |
978-989 |
JJ |
denotes |
horseradish |
| T9879 |
990-1011 |
JJ |
denotes |
peroxidase-conjugated |
| T9880 |
1012-1021 |
JJ |
denotes |
secondary |
| T9881 |
1022-1032 |
NNS |
denotes |
antibodies |
| T9882 |
1033-1037 |
VBD |
denotes |
were |
| T9883 |
1038-1043 |
VBN |
denotes |
added |
| T9884 |
1044-1047 |
CC |
denotes |
and |
| T9885 |
1048-1055 |
VBN |
denotes |
allowed |
| T9886 |
1056-1058 |
TO |
denotes |
to |
| T9887 |
1059-1067 |
VB |
denotes |
incubate |
| T9888 |
1068-1071 |
IN |
denotes |
for |
| T9889 |
1072-1073 |
CD |
denotes |
1 |
| T9890 |
1074-1078 |
NN |
denotes |
hour |
| T9891 |
1079-1081 |
IN |
denotes |
at |
| T9892 |
1082-1086 |
NN |
denotes |
room |
| T9893 |
1087-1098 |
NN |
denotes |
temperature |
| T9894 |
1098-1099 |
. |
denotes |
. |
| T9895 |
1100-1105 |
IN |
denotes |
After |
| T9896 |
1106-1111 |
NNP |
denotes |
TBS-T |
| T9897 |
1112-1119 |
VBG |
denotes |
washing |
| T9898 |
1119-1120 |
, |
denotes |
, |
| T9899 |
1121-1131 |
VBN |
denotes |
hybridized |
| T9900 |
1132-1137 |
NNS |
denotes |
bands |
| T9901 |
1138-1142 |
VBD |
denotes |
were |
| T9902 |
1143-1151 |
VBN |
denotes |
detected |
| T9903 |
1152-1156 |
IN |
denotes |
with |
| T9904 |
1157-1160 |
DT |
denotes |
the |
| T9905 |
1161-1169 |
JJ |
denotes |
enhanced |
| T9906 |
1170-1187 |
NN |
denotes |
chemiluminescence |
| T9907 |
1188-1189 |
-LRB- |
denotes |
( |
| T9908 |
1189-1192 |
NNP |
denotes |
ECL |
| T9909 |
1192-1193 |
-RRB- |
denotes |
) |
| T9910 |
1194-1203 |
NN |
denotes |
detection |
| T9911 |
1204-1207 |
NN |
denotes |
kit |
| T9912 |
1208-1211 |
CC |
denotes |
and |
| T9913 |
1212-1225 |
JJ |
denotes |
Hyperfilm-ECL |
| T9914 |
1226-1234 |
NNS |
denotes |
reagents |
| T9915 |
1235-1236 |
-LRB- |
denotes |
( |
| T9916 |
1236-1244 |
NNP |
denotes |
Amersham |
| T9917 |
1245-1254 |
NNP |
denotes |
Pharmacia |
| T9918 |
1254-1255 |
-RRB- |
denotes |
) |
| T9919 |
1255-1256 |
. |
denotes |
. |
| R5079 |
T9686 |
T9688 |
amod |
Western,analysis |
| R5080 |
T9687 |
T9688 |
compound |
blot,analysis |
| R5081 |
T9688 |
T9692 |
nsubj |
analysis,phosphorylated |
| R5082 |
T9689 |
T9688 |
prep |
of,analysis |
| R5083 |
T9690 |
T9689 |
pobj |
Akt,of |
| R5084 |
T9691 |
T9688 |
punct |
",",analysis |
| R5085 |
T9692 |
T9692 |
ROOT |
phosphorylated,phosphorylated |
| R5086 |
T9693 |
T9692 |
dobj |
Akt,phosphorylated |
| R5087 |
T9694 |
T9693 |
cc |
and,Akt |
| R5088 |
T9695 |
T9696 |
compound |
IκB-α,PBMC |
| R5089 |
T9696 |
T9693 |
conj |
PBMC,Akt |
| R5090 |
T9697 |
T9698 |
auxpass |
were,incubated |
| R5091 |
T9698 |
T9692 |
conj |
incubated,phosphorylated |
| R5092 |
T9699 |
T9698 |
prep |
with,incubated |
| R5093 |
T9700 |
T9699 |
pobj |
anti-CD3,with |
| R5094 |
T9701 |
T9705 |
punct |
(,ml |
| R5095 |
T9702 |
T9703 |
nummod |
10,μg |
| R5096 |
T9703 |
T9705 |
nmod |
μg,ml |
| R5097 |
T9704 |
T9705 |
compound |
/,ml |
| R5098 |
T9705 |
T9700 |
appos |
ml,anti-CD3 |
| R5099 |
T9706 |
T9705 |
punct |
),ml |
| R5100 |
T9707 |
T9698 |
prep |
in,incubated |
| R5101 |
T9708 |
T9709 |
det |
the,presence |
| R5102 |
T9709 |
T9707 |
pobj |
presence,in |
| R5103 |
T9710 |
T9709 |
cc |
or,presence |
| R5104 |
T9711 |
T9709 |
conj |
absence,presence |
| R5105 |
T9712 |
T9711 |
prep |
of,absence |
| R5106 |
T9713 |
T9712 |
pobj |
LY294002,of |
| R5107 |
T9714 |
T9716 |
punct |
(,μM |
| R5108 |
T9715 |
T9716 |
nummod |
20,μM |
| R5109 |
T9716 |
T9713 |
appos |
μM,LY294002 |
| R5110 |
T9717 |
T9716 |
punct |
),μM |
| R5111 |
T9718 |
T9692 |
punct |
.,phosphorylated |
| R5112 |
T9719 |
T9729 |
prep |
After,prepared |
| R5113 |
T9720 |
T9719 |
pobj |
incubation,After |
| R5114 |
T9721 |
T9729 |
prep |
for,prepared |
| R5115 |
T9722 |
T9723 |
nummod |
1,hour |
| R5116 |
T9723 |
T9721 |
pobj |
hour,for |
| R5117 |
T9724 |
T9729 |
punct |
",",prepared |
| R5118 |
T9725 |
T9727 |
amod |
whole,lysates |
| R5119 |
T9726 |
T9727 |
compound |
cell,lysates |
| R5120 |
T9727 |
T9729 |
nsubjpass |
lysates,prepared |
| R5121 |
T9728 |
T9729 |
auxpass |
were,prepared |
| R5122 |
T9729 |
T9729 |
ROOT |
prepared,prepared |
| R5123 |
T9730 |
T9729 |
prep |
from,prepared |
| R5124 |
T9731 |
T9733 |
nmod |
about,cells |
| R5125 |
T9732 |
T9733 |
nummod |
107,cells |
| R5126 |
T9733 |
T9730 |
pobj |
cells,from |
| R5127 |
T9734 |
T9733 |
prep |
by,cells |
| R5128 |
T9735 |
T9734 |
pobj |
homogenization,by |
| R5129 |
T9736 |
T9733 |
prep |
in,cells |
| R5130 |
T9737 |
T9739 |
det |
the,buffer |
| R5131 |
T9738 |
T9739 |
compound |
lysis,buffer |
| R5132 |
T9739 |
T9736 |
pobj |
buffer,in |
| R5133 |
T9740 |
T9729 |
punct |
",",prepared |
| R5134 |
T9741 |
T9729 |
cc |
and,prepared |
| R5135 |
T9742 |
T9729 |
conj |
centrifuged,prepared |
| R5136 |
T9743 |
T9742 |
prep |
at,centrifuged |
| R5137 |
T9744 |
T9745 |
nummod |
"14,000",r.p.m. |
| R5138 |
T9745 |
T9743 |
pobj |
r.p.m.,at |
| R5139 |
T9746 |
T9745 |
punct |
(,r.p.m. |
| R5140 |
T9747 |
T9748 |
nummod |
"19,000",g |
| R5141 |
T9748 |
T9745 |
appos |
g,r.p.m. |
| R5142 |
T9749 |
T9745 |
punct |
),r.p.m. |
| R5143 |
T9750 |
T9742 |
prep |
for,centrifuged |
| R5144 |
T9751 |
T9752 |
nummod |
15,min |
| R5145 |
T9752 |
T9750 |
pobj |
min,for |
| R5146 |
T9753 |
T9729 |
punct |
.,prepared |
| R5147 |
T9754 |
T9755 |
compound |
Protein,concentrations |
| R5148 |
T9755 |
T9760 |
nsubjpass |
concentrations,determined |
| R5149 |
T9756 |
T9755 |
prep |
in,concentrations |
| R5150 |
T9757 |
T9758 |
det |
the,supernatants |
| R5151 |
T9758 |
T9756 |
pobj |
supernatants,in |
| R5152 |
T9759 |
T9760 |
auxpass |
were,determined |
| R5153 |
T9760 |
T9760 |
ROOT |
determined,determined |
| R5154 |
T9761 |
T9760 |
prep |
with,determined |
| R5155 |
T9762 |
T9764 |
det |
the,method |
| R5156 |
T9763 |
T9764 |
compound |
Bradford,method |
| R5157 |
T9764 |
T9761 |
pobj |
method,with |
| R5158 |
T9765 |
T9766 |
punct |
(,Bio-Rad |
| R5159 |
T9766 |
T9764 |
appos |
Bio-Rad,method |
| R5160 |
T9767 |
T9766 |
punct |
",",Bio-Rad |
| R5161 |
T9768 |
T9766 |
conj |
Hercules,Bio-Rad |
| R5162 |
T9769 |
T9768 |
punct |
",",Hercules |
| R5163 |
T9770 |
T9768 |
conj |
CA,Hercules |
| R5164 |
T9771 |
T9770 |
punct |
",",CA |
| R5165 |
T9772 |
T9770 |
conj |
USA,CA |
| R5166 |
T9773 |
T9766 |
punct |
),Bio-Rad |
| R5167 |
T9774 |
T9760 |
punct |
.,determined |
| R5168 |
T9775 |
T9776 |
compound |
Protein,samples |
| R5169 |
T9776 |
T9778 |
nsubjpass |
samples,separated |
| R5170 |
T9777 |
T9778 |
auxpass |
were,separated |
| R5171 |
T9778 |
T9778 |
ROOT |
separated,separated |
| R5172 |
T9779 |
T9778 |
prep |
by,separated |
| R5173 |
T9780 |
T9781 |
nummod |
10,% |
| R5174 |
T9781 |
T9779 |
pobj |
%,by |
| R5175 |
T9782 |
T9783 |
compound |
SDS,PAGE |
| R5176 |
T9783 |
T9778 |
npadvmod |
PAGE,separated |
| R5177 |
T9784 |
T9778 |
cc |
and,separated |
| R5178 |
T9785 |
T9778 |
conj |
transferred,separated |
| R5179 |
T9786 |
T9785 |
prep |
to,transferred |
| R5180 |
T9787 |
T9789 |
det |
a,membrane |
| R5181 |
T9788 |
T9789 |
amod |
nitrocellulose,membrane |
| R5182 |
T9789 |
T9786 |
pobj |
membrane,to |
| R5183 |
T9790 |
T9793 |
punct |
(,Biotech |
| R5184 |
T9791 |
T9793 |
compound |
Amersham,Biotech |
| R5185 |
T9792 |
T9793 |
compound |
Pharmacia,Biotech |
| R5186 |
T9793 |
T9789 |
appos |
Biotech,membrane |
| R5187 |
T9794 |
T9793 |
punct |
",",Biotech |
| R5188 |
T9795 |
T9793 |
conj |
Uppsala,Biotech |
| R5189 |
T9796 |
T9795 |
punct |
",",Uppsala |
| R5190 |
T9797 |
T9795 |
conj |
Sweden,Uppsala |
| R5191 |
T9798 |
T9793 |
punct |
),Biotech |
| R5192 |
T9799 |
T9778 |
punct |
.,separated |
| R5193 |
T9800 |
T9806 |
prep |
For,preincubated |
| R5194 |
T9801 |
T9802 |
amod |
western,hybridization |
| R5195 |
T9802 |
T9800 |
pobj |
hybridization,For |
| R5196 |
T9803 |
T9806 |
punct |
",",preincubated |
| R5197 |
T9804 |
T9806 |
nsubjpass |
membrane,preincubated |
| R5198 |
T9805 |
T9806 |
auxpass |
was,preincubated |
| R5199 |
T9806 |
T9806 |
ROOT |
preincubated,preincubated |
| R5200 |
T9807 |
T9806 |
prep |
with,preincubated |
| R5201 |
T9808 |
T9809 |
nummod |
0.1,% |
| R5202 |
T9809 |
T9810 |
nsubj |
%,skimmed |
| R5203 |
T9810 |
T9806 |
conj |
skimmed,preincubated |
| R5204 |
T9811 |
T9810 |
dobj |
milk,skimmed |
| R5205 |
T9812 |
T9810 |
prep |
in,skimmed |
| R5206 |
T9813 |
T9814 |
compound |
TBS-T,buffer |
| R5207 |
T9814 |
T9812 |
pobj |
buffer,in |
| R5208 |
T9815 |
T9817 |
punct |
(,% |
| R5209 |
T9816 |
T9817 |
nummod |
0.1,% |
| R5210 |
T9817 |
T9837 |
nsubj |
%,phosphorylated |
| R5211 |
T9818 |
T9819 |
nummod |
Tween,20 |
| R5212 |
T9819 |
T9837 |
dep |
20,phosphorylated |
| R5213 |
T9820 |
T9819 |
prep |
in,20 |
| R5214 |
T9821 |
T9822 |
amod |
Tris-buffered,saline |
| R5215 |
T9822 |
T9820 |
pobj |
saline,in |
| R5216 |
T9823 |
T9819 |
punct |
),20 |
| R5217 |
T9824 |
T9819 |
prep |
at,20 |
| R5218 |
T9825 |
T9826 |
compound |
room,temperature |
| R5219 |
T9826 |
T9824 |
pobj |
temperature,at |
| R5220 |
T9827 |
T9819 |
prep |
for,20 |
| R5221 |
T9828 |
T9829 |
nummod |
2,hours |
| R5222 |
T9829 |
T9827 |
pobj |
hours,for |
| R5223 |
T9830 |
T9819 |
punct |
",",20 |
| R5224 |
T9831 |
T9833 |
advmod |
then,antibodies |
| R5225 |
T9832 |
T9833 |
amod |
primary,antibodies |
| R5226 |
T9833 |
T9819 |
appos |
antibodies,20 |
| R5227 |
T9834 |
T9833 |
prep |
against,antibodies |
| R5228 |
T9835 |
T9834 |
pobj |
Akt,against |
| R5229 |
T9836 |
T9819 |
punct |
",",20 |
| R5230 |
T9837 |
T9810 |
advcl |
phosphorylated,skimmed |
| R5231 |
T9838 |
T9837 |
dobj |
Akt,phosphorylated |
| R5232 |
T9839 |
T9838 |
cc |
and,Akt |
| R5233 |
T9840 |
T9838 |
conj |
IκB-α,Akt |
| R5234 |
T9841 |
T9845 |
punct |
(,Inc. |
| R5235 |
T9842 |
T9845 |
compound |
Cell,Inc. |
| R5236 |
T9843 |
T9845 |
compound |
Signaling,Inc. |
| R5237 |
T9844 |
T9845 |
compound |
Technology,Inc. |
| R5238 |
T9845 |
T9838 |
conj |
Inc.,Akt |
| R5239 |
T9846 |
T9845 |
punct |
",",Inc. |
| R5240 |
T9847 |
T9845 |
npadvmod |
Beverly,Inc. |
| R5241 |
T9848 |
T9847 |
punct |
",",Beverly |
| R5242 |
T9849 |
T9847 |
conj |
MA,Beverly |
| R5243 |
T9850 |
T9849 |
punct |
",",MA |
| R5244 |
T9851 |
T9849 |
npadvmod |
USA,MA |
| R5245 |
T9852 |
T9849 |
punct |
),MA |
| R5246 |
T9853 |
T9845 |
punct |
",",Inc. |
| R5247 |
T9854 |
T9837 |
prep |
diluted,phosphorylated |
| R5248 |
T9855 |
T9854 |
npadvmod |
1:1000,diluted |
| R5249 |
T9856 |
T9855 |
prep |
in,1:1000 |
| R5250 |
T9857 |
T9858 |
nummod |
5,% |
| R5251 |
T9858 |
T9859 |
compound |
%,BSA/TBS-T |
| R5252 |
T9859 |
T9856 |
pobj |
BSA/TBS-T,in |
| R5253 |
T9860 |
T9862 |
punct |
",",added |
| R5254 |
T9861 |
T9862 |
auxpass |
were,added |
| R5255 |
T9862 |
T9806 |
conj |
added,preincubated |
| R5256 |
T9863 |
T9862 |
cc |
and,added |
| R5257 |
T9864 |
T9862 |
conj |
incubated,added |
| R5258 |
T9865 |
T9864 |
advmod |
overnight,incubated |
| R5259 |
T9866 |
T9864 |
prep |
at,incubated |
| R5260 |
T9867 |
T9868 |
nummod |
4,° |
| R5261 |
T9868 |
T9866 |
pobj |
°,at |
| R5262 |
T9869 |
T9862 |
nsubj |
C,added |
| R5263 |
T9870 |
T9862 |
punct |
.,added |
| R5264 |
T9871 |
T9883 |
prep |
After,added |
| R5265 |
T9872 |
T9871 |
pcomp |
washing,After |
| R5266 |
T9873 |
T9874 |
nummod |
four,times |
| R5267 |
T9874 |
T9872 |
dobj |
times,washing |
| R5268 |
T9875 |
T9872 |
prep |
with,washing |
| R5269 |
T9876 |
T9875 |
pobj |
TBS-T,with |
| R5270 |
T9877 |
T9883 |
punct |
",",added |
| R5271 |
T9878 |
T9881 |
amod |
horseradish,antibodies |
| R5272 |
T9879 |
T9881 |
amod |
peroxidase-conjugated,antibodies |
| R5273 |
T9880 |
T9881 |
amod |
secondary,antibodies |
| R5274 |
T9881 |
T9883 |
nsubjpass |
antibodies,added |
| R5275 |
T9882 |
T9883 |
auxpass |
were,added |
| R5276 |
T9883 |
T9883 |
ROOT |
added,added |
| R5277 |
T9884 |
T9883 |
cc |
and,added |
| R5278 |
T9885 |
T9883 |
conj |
allowed,added |
| R5279 |
T9886 |
T9887 |
aux |
to,incubate |
| R5280 |
T9887 |
T9885 |
xcomp |
incubate,allowed |
| R5281 |
T9888 |
T9887 |
prep |
for,incubate |
| R5282 |
T9889 |
T9890 |
nummod |
1,hour |
| R5283 |
T9890 |
T9888 |
pobj |
hour,for |
| R5284 |
T9891 |
T9887 |
prep |
at,incubate |
| R5285 |
T9892 |
T9893 |
compound |
room,temperature |
| R5286 |
T9893 |
T9891 |
pobj |
temperature,at |
| R5287 |
T9894 |
T9883 |
punct |
.,added |
| R5288 |
T9895 |
T9902 |
prep |
After,detected |
| R5289 |
T9896 |
T9897 |
nsubj |
TBS-T,washing |
| R5290 |
T9897 |
T9895 |
pcomp |
washing,After |
| R5291 |
T9898 |
T9902 |
punct |
",",detected |
| R5292 |
T9899 |
T9900 |
amod |
hybridized,bands |
| R5293 |
T9900 |
T9902 |
nsubjpass |
bands,detected |
| R5294 |
T9901 |
T9902 |
auxpass |
were,detected |
| R5295 |
T9902 |
T9902 |
ROOT |
detected,detected |
| R5296 |
T9903 |
T9902 |
prep |
with,detected |
| R5297 |
T9904 |
T9906 |
det |
the,chemiluminescence |
| R5298 |
T9905 |
T9906 |
amod |
enhanced,chemiluminescence |
| R5299 |
T9906 |
T9903 |
pobj |
chemiluminescence,with |
| R5300 |
T9907 |
T9906 |
punct |
(,chemiluminescence |
| R5301 |
T9908 |
T9906 |
appos |
ECL,chemiluminescence |
| R5302 |
T9909 |
T9911 |
punct |
),kit |
| R5303 |
T9910 |
T9911 |
compound |
detection,kit |
| R5304 |
T9911 |
T9906 |
conj |
kit,chemiluminescence |
| R5305 |
T9912 |
T9911 |
cc |
and,kit |
| R5306 |
T9913 |
T9914 |
amod |
Hyperfilm-ECL,reagents |
| R5307 |
T9914 |
T9911 |
conj |
reagents,kit |
| R5308 |
T9915 |
T9917 |
punct |
(,Pharmacia |
| R5309 |
T9916 |
T9917 |
compound |
Amersham,Pharmacia |
| R5310 |
T9917 |
T9914 |
appos |
Pharmacia,reagents |
| R5311 |
T9918 |
T9914 |
punct |
),reagents |
| R5312 |
T9919 |
T9902 |
punct |
.,detected |
