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PMC:1064895 JSONTXT 32 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1443 0-9 VBN denotes Increased
T1444 10-24 JJ denotes interleukin-17
T1445 25-35 NN denotes production
T1446 36-39 IN denotes via
T1447 40-41 DT denotes a
T1448 42-58 NN denotes phosphoinositide
T1449 59-71 NN denotes 3-kinase/Akt
T1450 72-75 CC denotes and
T1451 76-83 JJ denotes nuclear
T1452 84-90 NN denotes factor
T1453 91-92 . denotes κ
T1454 92-103 JJ denotes B-dependent
T1455 104-111 NN denotes pathway
T1456 112-114 IN denotes in
T1457 115-123 NNS denotes patients
T1458 124-128 IN denotes with
T1459 129-139 JJ denotes rheumatoid
T1460 140-149 NNS denotes arthritis
T1461 160-172 JJ denotes Inflammatory
T1462 173-182 NNS denotes mediators
T1463 183-187 VBP denotes have
T1464 188-192 VBN denotes been
T1465 193-203 VBN denotes recognized
T1466 204-206 IN denotes as
T1467 207-212 VBG denotes being
T1468 213-222 JJ denotes important
T1469 223-225 IN denotes in
T1470 226-229 DT denotes the
T1471 230-242 NN denotes pathogenesis
T1472 243-245 IN denotes of
T1473 246-256 JJ denotes rheumatoid
T1474 257-266 NNS denotes arthritis
T1475 267-268 -LRB- denotes (
T1476 268-270 NNP denotes RA
T1477 270-271 -RRB- denotes )
T1478 271-272 . denotes .
T1479 273-284 NNP denotes Interleukin
T1480 285-286 -LRB- denotes (
T1481 286-288 NNP denotes IL
T1482 288-289 -RRB- denotes )
T1483 289-292 CD denotes -17
T1484 293-295 VBZ denotes is
T1485 296-298 DT denotes an
T1486 299-308 JJ denotes important
T1487 309-318 NN denotes regulator
T1488 319-321 IN denotes of
T1489 322-328 JJ denotes immune
T1490 329-332 CC denotes and
T1491 333-345 JJ denotes inflammatory
T1492 346-355 NNS denotes responses
T1493 355-356 , denotes ,
T1494 357-366 VBG denotes including
T1495 367-370 DT denotes the
T1496 371-380 NN denotes induction
T1497 381-383 IN denotes of
T1498 384-399 JJ denotes proinflammatory
T1499 400-409 NNS denotes cytokines
T1500 410-413 CC denotes and
T1501 414-426 JJ denotes osteoclastic
T1502 427-431 NN denotes bone
T1503 432-442 NN denotes resorption
T1504 442-443 . denotes .
T1505 444-452 NN denotes Evidence
T1506 453-456 IN denotes for
T1507 457-460 DT denotes the
T1508 461-471 NN denotes expression
T1509 472-475 CC denotes and
T1510 476-491 JJ denotes proinflammatory
T1511 492-500 NN denotes activity
T1512 501-503 IN denotes of
T1513 504-509 NNP denotes IL-17
T1514 510-513 VBZ denotes has
T1515 514-518 VBN denotes been
T1516 519-531 VBN denotes demonstrated
T1517 532-534 IN denotes in
T1518 535-537 NNP denotes RA
T1519 538-546 NN denotes synovium
T1520 547-550 CC denotes and
T1521 551-553 IN denotes in
T1522 554-560 NN denotes animal
T1523 561-567 NNS denotes models
T1524 568-570 IN denotes of
T1525 571-573 NNP denotes RA
T1526 573-574 . denotes .
T1527 575-583 IN denotes Although
T1528 584-588 DT denotes some
T1529 589-598 NNS denotes cytokines
T1530 599-600 -LRB- denotes (
T1531 600-605 NN denotes IL-15
T1532 606-609 CC denotes and
T1533 610-615 NN denotes IL-23
T1534 615-616 -RRB- denotes )
T1535 617-621 VBP denotes have
T1536 622-626 VBN denotes been
T1537 627-635 VBN denotes reported
T1538 636-638 TO denotes to
T1539 639-647 VB denotes regulate
T1540 648-653 JJ denotes IL-17
T1541 654-664 NN denotes production
T1542 664-665 , denotes ,
T1543 666-669 DT denotes the
T1544 670-683 JJ denotes intracellular
T1545 684-693 VBG denotes signaling
T1546 694-702 NNS denotes pathways
T1547 703-707 WDT denotes that
T1548 708-716 VBP denotes regulate
T1549 717-722 JJ denotes IL-17
T1550 723-733 NN denotes production
T1551 734-740 VBP denotes remain
T1552 741-748 JJ denotes unknown
T1553 748-749 . denotes .
T1554 750-752 IN denotes In
T1555 753-756 DT denotes the
T1556 757-764 JJ denotes present
T1557 765-770 NN denotes study
T1558 770-771 , denotes ,
T1559 772-774 PRP denotes we
T1560 775-787 VBD denotes investigated
T1561 788-791 DT denotes the
T1562 792-796 NN denotes role
T1563 797-799 IN denotes of
T1564 800-803 DT denotes the
T1565 804-820 NN denotes phosphoinositide
T1566 821-829 JJ denotes 3-kinase
T1567 830-831 -LRB- denotes (
T1568 831-835 NNP denotes PI3K
T1569 835-836 -RRB- denotes )
T1570 836-837 VBP denotes /
T1571 837-840 NNP denotes Akt
T1572 841-848 NN denotes pathway
T1573 849-851 IN denotes in
T1574 852-855 DT denotes the
T1575 856-866 NN denotes regulation
T1576 867-869 IN denotes of
T1577 870-875 JJ denotes IL-17
T1578 876-886 NN denotes production
T1579 887-889 IN denotes in
T1580 890-892 NNP denotes RA
T1581 892-893 . denotes .
T1582 894-904 JJ denotes Peripheral
T1583 905-910 NN denotes blood
T1584 911-922 NN denotes mononuclear
T1585 923-928 NNS denotes cells
T1586 929-930 -LRB- denotes (
T1587 930-934 NNP denotes PBMC
T1588 934-935 -RRB- denotes )
T1589 936-940 IN denotes from
T1590 941-949 NNS denotes patients
T1591 950-954 IN denotes with
T1592 955-957 NNP denotes RA
T1593 958-959 -LRB- denotes (
T1594 959-960 FW denotes n
T1595 961-962 SYM denotes =
T1596 963-965 CD denotes 24
T1597 965-966 -RRB- denotes )
T1598 967-971 VBD denotes were
T1599 972-981 VBN denotes separated
T1600 981-982 , denotes ,
T1601 983-987 RB denotes then
T1602 988-998 VBN denotes stimulated
T1603 999-1003 IN denotes with
T1604 1004-1011 JJ denotes various
T1605 1012-1018 NNS denotes agents
T1606 1019-1028 VBG denotes including
T1607 1029-1037 JJ denotes anti-CD3
T1608 1037-1038 , denotes ,
T1609 1039-1048 JJ denotes anti-CD28
T1610 1048-1049 , denotes ,
T1611 1050-1068 NN denotes phytohemagglutinin
T1612 1069-1070 -LRB- denotes (
T1613 1070-1073 NNP denotes PHA
T1614 1073-1074 -RRB- denotes )
T1615 1075-1078 CC denotes and
T1616 1079-1086 JJ denotes several
T1617 1087-1099 JJ denotes inflammatory
T1618 1100-1109 NNS denotes cytokines
T1619 1110-1113 CC denotes and
T1620 1114-1124 NNS denotes chemokines
T1621 1124-1125 . denotes .
T1622 1126-1131 NN denotes IL-17
T1623 1132-1138 NNS denotes levels
T1624 1139-1143 VBD denotes were
T1625 1144-1154 VBN denotes determined
T1626 1155-1157 IN denotes by
T1627 1158-1166 NN denotes sandwich
T1628 1167-1180 JJ denotes enzyme-linked
T1629 1181-1194 JJ denotes immunosorbent
T1630 1195-1200 NN denotes assay
T1631 1201-1204 CC denotes and
T1632 1205-1212 VB denotes reverse
T1633 1213-1226 NN denotes transcription
T1634 1227-1237 NN denotes polymerase
T1635 1238-1243 NN denotes chain
T1636 1244-1252 NN denotes reaction
T1637 1252-1253 . denotes .
T1638 1254-1257 DT denotes The
T1639 1258-1268 NN denotes production
T1640 1269-1271 IN denotes of
T1641 1272-1277 NNP denotes IL-17
T1642 1278-1281 VBD denotes was
T1643 1282-1295 RB denotes significantly
T1644 1296-1305 VBN denotes increased
T1645 1306-1308 IN denotes in
T1646 1309-1314 NNS denotes cells
T1647 1315-1322 VBN denotes treated
T1648 1323-1327 IN denotes with
T1649 1328-1336 JJ denotes anti-CD3
T1650 1337-1345 NN denotes antibody
T1651 1346-1350 IN denotes with
T1652 1351-1353 CC denotes or
T1653 1354-1361 IN denotes without
T1654 1362-1371 JJ denotes anti-CD28
T1655 1372-1375 CC denotes and
T1656 1376-1379 NNP denotes PHA
T1657 1380-1381 -LRB- denotes (
T1658 1381-1382 NNP denotes P
T1659 1383-1384 CD denotes <
T1660 1385-1389 CD denotes 0.05
T1661 1389-1390 -RRB- denotes )
T1662 1390-1391 . denotes .
T1663 1392-1397 IN denotes Among
T1664 1398-1404 VBN denotes tested
T1665 1405-1414 NNS denotes cytokines
T1666 1415-1418 CC denotes and
T1667 1419-1429 NNS denotes chemokines
T1668 1429-1430 , denotes ,
T1669 1431-1436 NNP denotes IL-15
T1670 1436-1437 , denotes ,
T1671 1438-1446 NN denotes monocyte
T1672 1447-1462 NN denotes chemoattractant
T1673 1463-1472 NN denotes protein-1
T1674 1473-1476 CC denotes and
T1675 1477-1481 NN denotes IL-6
T1676 1482-1493 VBD denotes upregulated
T1677 1494-1499 JJ denotes IL-17
T1678 1500-1510 NN denotes production
T1679 1511-1512 -LRB- denotes (
T1680 1512-1513 NNP denotes P
T1681 1514-1515 CD denotes <
T1682 1516-1520 CD denotes 0.05
T1683 1520-1521 -RRB- denotes )
T1684 1521-1522 , denotes ,
T1685 1523-1530 IN denotes whereas
T1686 1531-1536 NN denotes tumor
T1687 1537-1545 NN denotes necrosis
T1688 1546-1554 NN denotes factor-α
T1689 1554-1555 , denotes ,
T1690 1556-1561 NN denotes IL-1β
T1691 1561-1562 , denotes ,
T1692 1563-1568 NN denotes IL-18
T1693 1569-1571 CC denotes or
T1694 1572-1584 VBG denotes transforming
T1695 1585-1591 NN denotes growth
T1696 1592-1600 NN denotes factor-β
T1697 1601-1604 VBD denotes did
T1698 1605-1608 RB denotes not
T1699 1608-1609 . denotes .
T1700 1610-1615 NNP denotes IL-17
T1701 1616-1619 VBD denotes was
T1702 1620-1624 RB denotes also
T1703 1625-1633 VBN denotes detected
T1704 1634-1636 IN denotes in
T1705 1637-1640 DT denotes the
T1706 1641-1645 NNP denotes PBMC
T1707 1646-1648 IN denotes of
T1708 1649-1657 NNS denotes patients
T1709 1658-1662 IN denotes with
T1710 1663-1677 NNS denotes osteoarthritis
T1711 1677-1678 , denotes ,
T1712 1679-1682 CC denotes but
T1713 1683-1688 PRP$ denotes their
T1714 1689-1699 NN denotes expression
T1715 1700-1706 NNS denotes levels
T1716 1707-1711 VBD denotes were
T1717 1712-1716 RB denotes much
T1718 1717-1722 JJR denotes lower
T1719 1723-1727 IN denotes than
T1720 1728-1733 DT denotes those
T1721 1734-1736 IN denotes of
T1722 1737-1739 NNP denotes RA
T1723 1740-1744 NNP denotes PBMC
T1724 1744-1745 . denotes .
T1725 1746-1754 JJ denotes Anti-CD3
T1726 1755-1763 NN denotes antibody
T1727 1764-1773 VBD denotes activated
T1728 1774-1777 DT denotes the
T1729 1778-1786 JJ denotes PI3K/Akt
T1730 1787-1794 NN denotes pathway
T1731 1794-1795 : denotes ;
T1732 1796-1806 NN denotes activation
T1733 1807-1809 IN denotes of
T1734 1810-1814 DT denotes this
T1735 1815-1822 NN denotes pathway
T1736 1823-1831 VBD denotes resulted
T1737 1832-1834 IN denotes in
T1738 1835-1836 DT denotes a
T1739 1837-1847 JJ denotes pronounced
T1740 1848-1860 NN denotes augmentation
T1741 1861-1863 IN denotes of
T1742 1864-1871 JJ denotes nuclear
T1743 1872-1878 NN denotes factor
T1744 1879-1881 NNP denotes κB
T1745 1882-1883 -LRB- denotes (
T1746 1883-1888 NNP denotes NF-κB
T1747 1888-1889 -RRB- denotes )
T1748 1890-1901 JJ denotes DNA-binding
T1749 1902-1910 NN denotes activity
T1750 1910-1911 . denotes .
T1751 1912-1917 NN denotes IL-17
T1752 1918-1928 NN denotes production
T1753 1929-1931 IN denotes by
T1754 1932-1941 VBN denotes activated
T1755 1942-1944 NNP denotes RA
T1756 1945-1949 NNP denotes PBMC
T1757 1950-1952 VBZ denotes is
T1758 1953-1963 RB denotes completely
T1759 1964-1966 CC denotes or
T1760 1967-1973 RB denotes partly
T1761 1974-1981 VBN denotes blocked
T1762 1982-1984 IN denotes in
T1763 1985-1988 DT denotes the
T1764 1989-1997 NN denotes presence
T1765 1998-2000 IN denotes of
T1766 2001-2004 DT denotes the
T1767 2005-2010 NNP denotes NF-κB
T1768 2011-2020 NN denotes inhibitor
T1769 2021-2032 NN denotes pyrrolidine
T1770 2033-2048 NN denotes dithiocarbamate
T1771 2049-2052 CC denotes and
T1772 2053-2056 DT denotes the
T1773 2057-2065 JJ denotes PI3K/Akt
T1774 2066-2075 NN denotes inhibitor
T1775 2076-2086 NN denotes wortmannin
T1776 2087-2090 CC denotes and
T1777 2091-2099 NNP denotes LY294002
T1778 2099-2100 , denotes ,
T1779 2101-2113 RB denotes respectively
T1780 2113-2114 . denotes .
T1781 2115-2122 RB denotes However
T1782 2122-2123 , denotes ,
T1783 2124-2134 NN denotes inhibition
T1784 2135-2137 IN denotes of
T1785 2138-2147 NN denotes activator
T1786 2148-2157 NN denotes protein-1
T1787 2158-2161 CC denotes and
T1788 2162-2175 JJ denotes extracellular
T1789 2176-2192 JJ denotes signal-regulated
T1790 2193-2199 NN denotes kinase
T1791 2200-2203 CD denotes 1/2
T1792 2204-2207 VBD denotes did
T1793 2208-2211 RB denotes not
T1794 2212-2218 VB denotes affect
T1795 2219-2224 JJ denotes IL-17
T1796 2225-2235 NN denotes production
T1797 2235-2236 . denotes .
T1798 2237-2242 DT denotes These
T1799 2243-2250 NNS denotes results
T1800 2251-2258 VBP denotes suggest
T1801 2259-2263 IN denotes that
T1802 2264-2270 NN denotes signal
T1803 2271-2283 NN denotes transduction
T1804 2284-2292 NNS denotes pathways
T1805 2293-2302 JJ denotes dependent
T1806 2303-2305 IN denotes on
T1807 2306-2314 NNP denotes PI3K/Akt
T1808 2315-2318 CC denotes and
T1809 2319-2324 NNP denotes NF-κB
T1810 2325-2328 VBP denotes are
T1811 2329-2337 VBN denotes involved
T1812 2338-2340 IN denotes in
T1813 2341-2344 DT denotes the
T1814 2345-2359 NN denotes overproduction
T1815 2360-2362 IN denotes of
T1816 2363-2366 DT denotes the
T1817 2367-2370 JJ denotes key
T1818 2371-2383 JJ denotes inflammatory
T1819 2384-2392 NN denotes cytokine
T1820 2393-2398 NNP denotes IL-17
T1821 2399-2401 IN denotes in
T1822 2402-2404 NNP denotes RA
T1823 2404-2405 . denotes .
T3852 2420-2430 JJ denotes Rheumatoid
T3853 2431-2440 NNS denotes arthritis
T3854 2441-2442 -LRB- denotes (
T3855 2442-2444 NNP denotes RA
T3856 2444-2445 -RRB- denotes )
T3857 2446-2448 VBZ denotes is
T3858 2449-2462 VBN denotes characterized
T3859 2463-2465 IN denotes by
T3860 2466-2479 NNS denotes infiltrations
T3861 2480-2482 IN denotes of
T3862 2483-2494 NNS denotes macrophages
T3863 2495-2498 CC denotes and
T3864 2499-2500 NNP denotes T
T3865 2501-2506 NNS denotes cells
T3866 2507-2511 IN denotes into
T3867 2512-2515 DT denotes the
T3868 2516-2521 JJ denotes joint
T3869 2521-2522 , denotes ,
T3870 2523-2526 CC denotes and
T3871 2527-2535 JJ denotes synovial
T3872 2536-2547 NN denotes hyperplasia
T3873 2547-2548 . denotes .
T3874 2549-2564 JJ denotes Proinflammatory
T3875 2565-2574 NNS denotes cytokines
T3876 2575-2583 VBN denotes released
T3877 2584-2588 IN denotes from
T3878 2589-2594 DT denotes these
T3879 2595-2600 NNS denotes cells
T3880 2601-2604 VBP denotes are
T3881 2605-2610 VBN denotes known
T3882 2611-2613 TO denotes to
T3883 2614-2616 VB denotes be
T3884 2617-2626 JJ denotes important
T3885 2627-2629 IN denotes in
T3886 2630-2633 DT denotes the
T3887 2634-2645 NN denotes destruction
T3888 2646-2648 IN denotes of
T3889 2649-2655 NNS denotes joints
T3890 2656-2658 IN denotes in
T3891 2659-2661 NNP denotes RA
T3892 2662-2663 NNP denotes [
T3893 2663-2664 CD denotes 1
T3894 2664-2665 NNP denotes ]
T3895 2665-2666 . denotes .
T3896 2667-2670 DT denotes The
T3897 2671-2680 JJ denotes favorable
T3898 2681-2689 JJ denotes clinical
T3899 2690-2698 NNS denotes benefits
T3900 2699-2707 VBN denotes obtained
T3901 2708-2712 IN denotes with
T3902 2713-2723 NNS denotes inhibitors
T3903 2724-2726 IN denotes of
T3904 2727-2732 NN denotes tumor
T3905 2733-2741 NN denotes necrosis
T3906 2742-2748 NN denotes factor
T3907 2749-2750 -LRB- denotes (
T3908 2750-2753 NNP denotes TNF
T3909 2753-2754 -RRB- denotes )
T3910 2754-2755 : denotes -
T3911 2755-2756 VBN denotes α
T3912 2756-2757 -RRB- denotes )
T3913 2758-2761 CC denotes and
T3914 2762-2773 NN denotes interleukin
T3915 2774-2775 -LRB- denotes (
T3916 2775-2777 NNP denotes IL
T3917 2777-2778 -RRB- denotes )
T3918 2778-2780 CD denotes -1
T3919 2781-2788 VBP denotes suggest
T3920 2789-2793 IN denotes that
T3921 2794-2797 DT denotes the
T3922 2798-2806 NN denotes blockade
T3923 2807-2809 IN denotes of
T3924 2810-2813 JJ denotes key
T3925 2814-2826 JJ denotes inflammatory
T3926 2827-2836 NNS denotes cytokines
T3927 2837-2840 VBZ denotes has
T3928 2841-2845 VBN denotes been
T3929 2846-2849 DT denotes the
T3930 2850-2859 JJ denotes important
T3931 2860-2865 NN denotes issue
T3932 2866-2868 IN denotes in
T3933 2869-2872 DT denotes the
T3934 2873-2884 NN denotes development
T3935 2885-2887 IN denotes of
T3936 2888-2891 JJ denotes new
T3937 2892-2903 JJ denotes therapeutic
T3938 2904-2916 NNS denotes applications
T3939 2917-2918 JJ denotes [
T3940 2918-2919 CD denotes 2
T3941 2919-2920 NN denotes ]
T3942 2920-2921 . denotes .
T3943 2922-2923 DT denotes A
T3944 2924-2930 JJ denotes little
T3945 2931-2935 IN denotes over
T3946 2936-2937 DT denotes a
T3947 2938-2944 NN denotes decade
T3948 2945-2948 RB denotes ago
T3949 2948-2949 , denotes ,
T3950 2950-2953 DT denotes the
T3951 2954-2961 NN denotes primacy
T3952 2962-2964 IN denotes of
T3953 2965-2966 NNP denotes T
T3954 2967-2972 NNS denotes cells
T3955 2973-2975 IN denotes in
T3956 2976-2979 DT denotes the
T3957 2980-2992 NN denotes pathogenesis
T3958 2993-2995 IN denotes of
T3959 2996-3006 JJ denotes autoimmune
T3960 3007-3014 NN denotes disease
T3961 3015-3019 JJ denotes such
T3962 3020-3022 IN denotes as
T3963 3023-3025 NNP denotes RA
T3964 3026-3029 VBD denotes was
T3965 3030-3040 JJ denotes undisputed
T3966 3041-3048 IN denotes because
T3967 3049-3053 PRP denotes they
T3968 3054-3057 VBP denotes are
T3969 3058-3061 DT denotes the
T3970 3062-3069 JJS denotes largest
T3971 3070-3074 NN denotes cell
T3972 3075-3085 NN denotes population
T3973 3086-3098 VBG denotes infiltrating
T3974 3099-3102 DT denotes the
T3975 3103-3111 NN denotes synovium
T3976 3111-3112 . denotes .
T3977 3113-3120 RB denotes However
T3978 3120-3121 , denotes ,
T3979 3122-3123 DT denotes a
T3980 3124-3130 NN denotes series
T3981 3131-3133 IN denotes of
T3982 3134-3141 NNS denotes studies
T3983 3142-3154 VBD denotes demonstrated
T3984 3155-3162 NN denotes paucity
T3985 3163-3165 IN denotes of
T3986 3166-3167 NNP denotes T
T3987 3168-3180 JJ denotes cell-derived
T3988 3181-3190 NNS denotes cytokines
T3989 3191-3195 JJ denotes such
T3990 3196-3198 IN denotes as
T3991 3199-3203 NN denotes IL-2
T3992 3204-3207 CC denotes and
T3993 3208-3220 NN denotes interferon-γ
T3994 3221-3223 IN denotes in
T3995 3224-3227 DT denotes the
T3996 3228-3234 NNS denotes joints
T3997 3235-3237 IN denotes of
T3998 3238-3240 NNP denotes RA
T3999 3240-3241 , denotes ,
T4000 3242-3249 IN denotes whereas
T4001 3250-3260 NN denotes macrophage
T4002 3261-3264 CC denotes and
T4003 3265-3275 NN denotes fibroblast
T4004 3276-3285 NNS denotes cytokines
T4005 3286-3295 VBG denotes including
T4006 3296-3300 NNP denotes IL-1
T4007 3300-3301 , denotes ,
T4008 3302-3306 NNP denotes IL-6
T4009 3306-3307 , denotes ,
T4010 3308-3313 NNP denotes IL-15
T4011 3313-3314 , denotes ,
T4012 3315-3320 NNP denotes IL-18
T4013 3321-3324 CC denotes and
T4014 3325-3330 NNP denotes TNF-α
T4015 3331-3335 VBD denotes were
T4016 3336-3344 JJ denotes abundant
T4017 3345-3347 IN denotes in
T4018 3348-3358 JJ denotes rheumatoid
T4019 3359-3367 NN denotes synovium
T4020 3367-3368 . denotes .
T4021 3369-3373 DT denotes This
T4022 3374-3381 NN denotes paradox
T4023 3382-3385 VBZ denotes has
T4024 3386-3396 VBN denotes questioned
T4025 3397-3400 DT denotes the
T4026 3401-3405 NN denotes role
T4027 3406-3408 IN denotes of
T4028 3409-3410 NNP denotes T
T4029 3411-3416 NNS denotes cells
T4030 3417-3419 IN denotes in
T4031 3420-3423 DT denotes the
T4032 3424-3436 NN denotes pathogenesis
T4033 3437-3439 IN denotes of
T4034 3440-3442 NNP denotes RA
T4035 3443-3444 NNP denotes [
T4036 3444-3445 CD denotes 3
T4037 3445-3446 NNP denotes ]
T4038 3446-3447 . denotes .
T4039 3448-3455 IN denotes Because
T4040 3456-3458 PRP denotes we
T4041 3459-3463 VBP denotes have
T4042 3464-3471 RB denotes already
T4043 3472-3484 VBN denotes demonstrated
T4044 3485-3488 DT denotes the
T4045 3489-3497 JJ denotes enhanced
T4046 3498-3511 NN denotes proliferation
T4047 3512-3514 IN denotes of
T4048 3515-3522 NN denotes antigen
T4049 3523-3531 JJ denotes specific
T4050 3532-3533 NNP denotes T
T4051 3534-3539 NNS denotes cells
T4052 3539-3540 , denotes ,
T4053 3541-3551 RB denotes especially
T4054 3552-3554 TO denotes to
T4055 3555-3559 NN denotes type
T4056 3560-3562 NNP denotes II
T4057 3563-3571 NN denotes collagen
T4058 3571-3572 , denotes ,
T4059 3573-3576 CC denotes and
T4060 3577-3580 DT denotes the
T4061 3581-3588 NN denotes skewing
T4062 3589-3591 IN denotes of
T4063 3592-3593 NNP denotes T
T4064 3594-3600 NN denotes helper
T4065 3601-3605 NN denotes type
T4066 3606-3607 CD denotes 1
T4067 3608-3609 -LRB- denotes (
T4068 3609-3612 NN denotes Th1
T4069 3612-3613 -RRB- denotes )
T4070 3614-3623 NNS denotes cytokines
T4071 3624-3626 IN denotes in
T4072 3627-3629 NNP denotes RA
T4073 3630-3631 NNP denotes [
T4074 3631-3632 CD denotes 4
T4075 3632-3633 NNP denotes ]
T4076 3633-3634 , denotes ,
T4077 3635-3638 DT denotes the
T4078 3639-3643 NN denotes role
T4079 3644-3646 IN denotes of
T4080 3647-3648 NNP denotes T
T4081 3649-3654 NNS denotes cells
T4082 3655-3660 VBZ denotes needs
T4083 3661-3663 TO denotes to
T4084 3664-3666 VB denotes be
T4085 3667-3677 VBN denotes elucidated
T4086 3678-3680 IN denotes in
T4087 3681-3690 JJ denotes different
T4088 3691-3698 NNS denotes aspects
T4089 3698-3699 . denotes .
T4090 3700-3705 NNP denotes IL-17
T4091 3706-3708 VBZ denotes is
T4092 3709-3712 CD denotes one
T4093 3713-3715 IN denotes of
T4094 3716-3719 DT denotes the
T4095 3720-3732 JJ denotes inflammatory
T4096 3733-3742 NNS denotes cytokines
T4097 3743-3751 VBD denotes secreted
T4098 3752-3758 RB denotes mainly
T4099 3759-3761 IN denotes by
T4100 3762-3771 VBN denotes activated
T4101 3772-3773 NNP denotes T
T4102 3774-3779 NNS denotes cells
T4103 3779-3780 , denotes ,
T4104 3781-3786 WDT denotes which
T4105 3787-3790 MD denotes can
T4106 3791-3797 VB denotes induce
T4107 3798-3802 NNP denotes IL-6
T4108 3803-3806 CC denotes and
T4109 3807-3811 NNP denotes IL-8
T4110 3812-3814 IN denotes by
T4111 3815-3826 NNS denotes fibroblasts
T4112 3827-3828 VBP denotes [
T4113 3828-3829 CD denotes 5
T4114 3829-3830 NNP denotes ]
T4115 3830-3831 . denotes .
T4116 3832-3836 DT denotes This
T4117 3837-3845 NN denotes cytokine
T4118 3846-3848 VBZ denotes is
T4119 3849-3851 IN denotes of
T4120 3852-3860 NN denotes interest
T4121 3861-3864 IN denotes for
T4122 3865-3868 CD denotes two
T4123 3869-3874 JJ denotes major
T4124 3875-3882 NNS denotes reasons
T4125 3882-3883 : denotes :
T4126 3884-3889 RB denotes first
T4127 3889-3890 , denotes ,
T4128 3891-3900 RB denotes similarly
T4129 3901-3903 TO denotes to
T4130 3904-3909 NNP denotes TNF-α
T4131 3910-3913 CC denotes and
T4132 3914-3918 NNP denotes IL-1
T4133 3918-3919 , denotes ,
T4134 3920-3925 NNP denotes IL-17
T4135 3926-3929 VBZ denotes has
T4136 3930-3945 JJ denotes proinflammatory
T4137 3946-3956 NNS denotes properties
T4138 3956-3957 : denotes ;
T4139 3958-3964 JJ denotes second
T4140 3964-3965 , denotes ,
T4141 3966-3968 PRP denotes it
T4142 3969-3971 VBZ denotes is
T4143 3972-3980 VBN denotes produced
T4144 3981-3983 IN denotes by
T4145 3984-3985 NNP denotes T
T4146 3986-3991 NNS denotes cells
T4147 3992-3993 NNP denotes [
T4148 3993-3994 CD denotes 6
T4149 3994-3995 NNP denotes ]
T4150 3995-3996 . denotes .
T4151 3997-4003 JJ denotes Recent
T4152 4004-4016 NNS denotes observations
T4153 4017-4029 VBD denotes demonstrated
T4154 4030-4034 IN denotes that
T4155 4035-4040 NNP denotes IL-17
T4156 4041-4044 MD denotes can
T4157 4045-4049 RB denotes also
T4158 4050-4058 VB denotes activate
T4159 4059-4071 JJ denotes osteoclastic
T4160 4072-4076 NN denotes bone
T4161 4077-4087 NN denotes resorption
T4162 4088-4090 IN denotes by
T4163 4091-4094 DT denotes the
T4164 4095-4104 NN denotes induction
T4165 4105-4107 IN denotes of
T4166 4108-4113 NNP denotes RANKL
T4167 4114-4115 -LRB- denotes (
T4168 4115-4123 NN denotes receptor
T4169 4124-4133 NN denotes activator
T4170 4134-4136 IN denotes of
T4171 4137-4144 JJ denotes nuclear
T4172 4145-4151 NN denotes factor
T4173 4152-4154 NNP denotes κB
T4174 4155-4156 NNP denotes [
T4175 4156-4161 NNP denotes NF-κB
T4176 4161-4162 NNP denotes ]
T4177 4163-4169 NN denotes ligand
T4178 4169-4170 -RRB- denotes )
T4179 4170-4171 , denotes ,
T4180 4172-4177 WDT denotes which
T4181 4178-4180 VBZ denotes is
T4182 4181-4189 VBN denotes involved
T4183 4190-4192 IN denotes in
T4184 4193-4197 NN denotes bony
T4185 4198-4205 NN denotes erosion
T4186 4206-4208 IN denotes in
T4187 4209-4211 NNP denotes RA
T4188 4212-4213 NNP denotes [
T4189 4213-4214 CD denotes 7
T4190 4214-4215 NNP denotes ]
T4191 4215-4216 . denotes .
T4192 4217-4219 PRP denotes It
T4193 4220-4224 RB denotes also
T4194 4225-4235 VBZ denotes stimulates
T4195 4236-4239 DT denotes the
T4196 4240-4250 NN denotes production
T4197 4251-4253 IN denotes of
T4198 4254-4258 NN denotes IL-6
T4199 4259-4262 CC denotes and
T4200 4263-4271 NN denotes leukemia
T4201 4272-4282 JJ denotes inhibitory
T4202 4283-4289 NN denotes factor
T4203 4290-4292 IN denotes by
T4204 4293-4305 NNS denotes synoviocytes
T4205 4305-4306 , denotes ,
T4206 4307-4310 CC denotes and
T4207 4311-4313 IN denotes of
T4208 4314-4327 NN denotes prostaglandin
T4209 4328-4330 CD denotes E2
T4210 4331-4334 CC denotes and
T4211 4335-4341 JJ denotes nitric
T4212 4342-4347 NN denotes oxide
T4213 4348-4350 IN denotes by
T4214 4351-4363 NNS denotes chondrocytes
T4215 4363-4364 , denotes ,
T4216 4365-4368 CC denotes and
T4217 4369-4372 VBZ denotes has
T4218 4373-4376 DT denotes the
T4219 4377-4384 NN denotes ability
T4220 4385-4387 TO denotes to
T4221 4388-4401 VB denotes differentiate
T4222 4402-4405 CC denotes and
T4223 4406-4414 VB denotes activate
T4224 4415-4418 DT denotes the
T4225 4419-4428 JJ denotes dendritic
T4226 4429-4434 NNS denotes cells
T4227 4435-4436 NNP denotes [
T4228 4436-4440 CD denotes 8-10
T4229 4440-4441 NNP denotes ]
T4230 4441-4442 . denotes .
T4231 4443-4449 NNS denotes Levels
T4232 4450-4452 IN denotes of
T4233 4453-4458 NNP denotes IL-17
T4234 4459-4461 IN denotes in
T4235 4462-4470 JJ denotes synovial
T4236 4471-4477 NNS denotes fluids
T4237 4478-4482 VBD denotes were
T4238 4483-4496 RB denotes significantly
T4239 4497-4503 JJR denotes higher
T4240 4504-4506 IN denotes in
T4241 4507-4515 NNS denotes patients
T4242 4516-4520 IN denotes with
T4243 4521-4523 NNP denotes RA
T4244 4524-4528 IN denotes than
T4245 4529-4531 IN denotes in
T4246 4532-4540 NNS denotes patients
T4247 4541-4545 IN denotes with
T4248 4546-4560 NNS denotes osteoarthritis
T4249 4561-4562 -LRB- denotes (
T4250 4562-4564 NNP denotes OA
T4251 4564-4565 -RRB- denotes )
T4252 4565-4566 , denotes ,
T4253 4567-4570 CC denotes and
T4254 4571-4573 PRP denotes it
T4255 4574-4577 VBD denotes was
T4256 4578-4586 VBN denotes produced
T4257 4587-4589 IN denotes by
T4258 4590-4593 NNP denotes CD4
T4259 4593-4594 NNP denotes +
T4260 4595-4596 NNP denotes T
T4261 4597-4602 NNS denotes cells
T4262 4603-4605 IN denotes in
T4263 4606-4609 DT denotes the
T4264 4610-4618 NN denotes synovium
T4265 4619-4620 NNP denotes [
T4266 4620-4625 CD denotes 11,12
T4267 4625-4626 NNP denotes ]
T4268 4626-4627 . denotes .
T4269 4628-4633 CD denotes IL-15
T4270 4633-4634 , denotes ,
T4271 4635-4643 VBN denotes secreted
T4272 4644-4648 IN denotes from
T4273 4649-4658 VBN denotes activated
T4274 4659-4670 NNS denotes macrophages
T4275 4670-4671 , denotes ,
T4276 4672-4675 VBZ denotes has
T4277 4676-4680 VBN denotes been
T4278 4681-4689 VBN denotes reported
T4279 4690-4692 TO denotes to
T4280 4693-4695 VB denotes be
T4281 4696-4698 DT denotes an
T4282 4699-4708 JJ denotes important
T4283 4709-4716 NN denotes trigger
T4284 4717-4719 IN denotes of
T4285 4720-4725 JJ denotes IL-17
T4286 4726-4736 NN denotes production
T4287 4737-4739 IN denotes in
T4288 4740-4742 NNP denotes RA
T4289 4743-4753 JJ denotes peripheral
T4290 4754-4759 NN denotes blood
T4291 4760-4771 NN denotes mononuclear
T4292 4772-4777 NNS denotes cells
T4293 4778-4779 -LRB- denotes (
T4294 4779-4783 NNP denotes PBMC
T4295 4783-4784 -RRB- denotes )
T4296 4785-4787 IN denotes by
T4297 4788-4800 NN denotes cyclosporine
T4298 4801-4804 CC denotes and
T4299 4805-4812 JJ denotes steroid
T4300 4813-4822 JJ denotes sensitive
T4301 4823-4831 NNS denotes pathways
T4302 4832-4833 NNP denotes [
T4303 4833-4835 CD denotes 13
T4304 4835-4836 NNP denotes ]
T4305 4836-4837 . denotes .
T4306 4838-4846 RB denotes Recently
T4307 4846-4847 , denotes ,
T4308 4848-4854 NNP denotes Happel
T4309 4855-4858 CC denotes and
T4310 4859-4869 NNS denotes colleagues
T4311 4870-4874 RB denotes also
T4312 4875-4881 VBD denotes showed
T4313 4882-4886 IN denotes that
T4314 4887-4892 NN denotes IL-23
T4315 4893-4898 MD denotes could
T4316 4899-4901 VB denotes be
T4317 4902-4904 DT denotes an
T4318 4905-4914 JJ denotes efficient
T4319 4915-4922 NN denotes trigger
T4320 4923-4925 IN denotes of
T4321 4926-4931 JJ denotes IL-17
T4322 4932-4942 NN denotes production
T4323 4943-4947 IN denotes from
T4324 4948-4952 DT denotes both
T4325 4953-4956 CD denotes CD4
T4326 4956-4957 NN denotes +
T4327 4958-4961 CC denotes and
T4328 4962-4965 CD denotes CD8
T4329 4965-4966 CD denotes +
T4330 4967-4968 NNP denotes T
T4331 4969-4974 NNS denotes cells
T4332 4975-4976 NNP denotes [
T4333 4976-4978 CD denotes 14
T4334 4978-4979 NNP denotes ]
T4335 4979-4980 . denotes .
T4336 4981-4989 IN denotes Although
T4337 4990-4993 DT denotes the
T4338 4994-5006 NN denotes contribution
T4339 5007-5009 IN denotes of
T4340 5010-5015 NNP denotes IL-17
T4341 5016-5018 IN denotes in
T4342 5019-5024 JJ denotes joint
T4343 5025-5037 NN denotes inflammation
T4344 5038-5040 IN denotes in
T4345 5041-5043 NNP denotes RA
T4346 5044-5047 VBZ denotes has
T4347 5048-5052 VBN denotes been
T4348 5053-5063 VBN denotes documented
T4349 5064-5066 IN denotes in
T4350 5067-5074 JJR denotes earlier
T4351 5075-5082 NNS denotes studies
T4352 5083-5084 NNP denotes [
T4353 5084-5092 CD denotes 12,15,16
T4354 5092-5093 NNP denotes ]
T4355 5093-5094 , denotes ,
T4356 5095-5098 DT denotes the
T4357 5099-5112 JJ denotes intracellular
T4358 5113-5119 NN denotes signal
T4359 5120-5132 NN denotes transduction
T4360 5133-5140 NN denotes pathway
T4361 5141-5144 IN denotes for
T4362 5145-5150 JJ denotes IL-17
T4363 5151-5161 NN denotes production
T4364 5162-5169 VBZ denotes remains
T4365 5170-5179 JJ denotes uncertain
T4366 5179-5180 . denotes .
T4367 5181-5183 IN denotes In
T4368 5184-5187 DT denotes the
T4369 5188-5195 JJ denotes present
T4370 5196-5201 NN denotes study
T4371 5202-5204 PRP denotes we
T4372 5205-5209 VBD denotes used
T4373 5210-5217 JJ denotes various
T4374 5218-5225 NNS denotes stimuli
T4375 5226-5228 TO denotes to
T4376 5229-5240 VB denotes investigate
T4377 5241-5246 JJ denotes IL-17
T4378 5247-5257 NN denotes production
T4379 5258-5260 IN denotes in
T4380 5261-5265 NNP denotes PBMC
T4381 5266-5268 IN denotes of
T4382 5269-5277 NNS denotes patients
T4383 5278-5282 IN denotes with
T4384 5283-5285 NNP denotes RA
T4385 5286-5289 CC denotes and
T4386 5290-5293 PRP$ denotes its
T4387 5294-5303 VBG denotes signaling
T4388 5304-5316 NN denotes transduction
T4389 5317-5324 NN denotes pathway
T4390 5324-5325 . denotes .
T4391 5326-5328 PRP denotes We
T4392 5329-5334 VBD denotes found
T4393 5335-5339 IN denotes that
T4394 5340-5343 DT denotes the
T4395 5344-5357 JJ denotes intracellular
T4396 5358-5367 VBG denotes signaling
T4397 5368-5375 NN denotes pathway
T4398 5376-5385 VBG denotes involving
T4399 5386-5402 NN denotes phosphoinositide
T4400 5403-5411 JJ denotes 3-kinase
T4401 5412-5413 -LRB- denotes (
T4402 5413-5417 NNP denotes PI3K
T4403 5417-5418 -RRB- denotes )
T4404 5418-5419 VBP denotes /
T4405 5419-5422 NNP denotes Akt
T4406 5423-5426 CC denotes and
T4407 5427-5432 NNP denotes NF-κB
T4408 5433-5438 MD denotes might
T4409 5439-5441 VB denotes be
T4410 5442-5450 VBN denotes involved
T4411 5451-5453 IN denotes in
T4412 5454-5457 DT denotes the
T4413 5458-5472 NN denotes overproduction
T4414 5473-5475 IN denotes of
T4415 5476-5479 DT denotes the
T4416 5480-5483 JJ denotes key
T4417 5484-5496 JJ denotes inflammatory
T4418 5497-5505 NN denotes cytokine
T4419 5506-5511 NNP denotes IL-17
T4420 5512-5514 IN denotes in
T4421 5515-5517 NNP denotes RA
T4422 5517-5518 . denotes .
T4423 5519-5524 DT denotes These
T4424 5525-5532 NNS denotes results
T4425 5533-5538 MD denotes might
T4426 5539-5546 VB denotes provide
T4427 5547-5550 JJ denotes new
T4428 5551-5559 NNS denotes insights
T4429 5560-5564 IN denotes into
T4430 5565-5568 DT denotes the
T4431 5569-5581 NN denotes pathogenesis
T4432 5582-5584 IN denotes of
T4433 5585-5587 NNP denotes RA
T4434 5588-5591 CC denotes and
T4435 5592-5598 JJ denotes future
T4436 5599-5609 NNS denotes directions
T4437 5610-5613 IN denotes for
T4438 5614-5617 JJ denotes new
T4439 5618-5629 JJ denotes therapeutic
T4440 5630-5640 NNS denotes strategies
T4441 5641-5643 IN denotes in
T4442 5644-5646 NNP denotes RA
T4443 5646-5647 . denotes .
T5038 5672-5680 NNS denotes Patients
T5039 5681-5689 VBN denotes Informed
T5040 5690-5697 NN denotes consent
T5041 5698-5701 VBD denotes was
T5042 5702-5710 VBN denotes obtained
T5043 5711-5715 IN denotes from
T5044 5716-5718 CD denotes 24
T5045 5719-5727 NNS denotes patients
T5046 5728-5729 -LRB- denotes (
T5047 5729-5730 CD denotes 5
T5048 5731-5734 NNS denotes men
T5049 5735-5738 CC denotes and
T5050 5739-5741 CD denotes 19
T5051 5742-5747 NNS denotes women
T5052 5747-5748 -RRB- denotes )
T5053 5749-5753 IN denotes with
T5054 5754-5756 NNP denotes RA
T5055 5757-5760 WP denotes who
T5056 5761-5770 VBD denotes fulfilled
T5057 5771-5774 DT denotes the
T5058 5775-5779 CD denotes 1987
T5059 5780-5787 VBN denotes revised
T5060 5788-5796 NNS denotes criteria
T5061 5797-5799 IN denotes of
T5062 5800-5803 DT denotes the
T5063 5804-5812 JJ denotes American
T5064 5813-5820 NNP denotes College
T5065 5821-5823 IN denotes of
T5066 5824-5836 NNP denotes Rheumatology
T5067 5837-5838 -LRB- denotes (
T5068 5838-5846 RB denotes formerly
T5069 5847-5850 DT denotes the
T5070 5851-5859 JJ denotes American
T5071 5860-5870 NNP denotes Rheumatism
T5072 5871-5882 NNP denotes Association
T5073 5882-5883 -RRB- denotes )
T5074 5884-5885 NNP denotes [
T5075 5885-5887 CD denotes 17
T5076 5887-5888 NNP denotes ]
T5077 5888-5889 . denotes .
T5078 5890-5893 DT denotes The
T5079 5894-5897 NN denotes age
T5080 5898-5900 IN denotes of
T5081 5901-5904 DT denotes the
T5082 5905-5913 NNS denotes patients
T5083 5914-5918 IN denotes with
T5084 5919-5921 NNP denotes RA
T5085 5922-5925 VBD denotes was
T5086 5926-5928 CD denotes 50
T5087 5929-5930 NN denotes ±
T5088 5931-5932 CD denotes 8
T5089 5933-5934 -LRB- denotes (
T5090 5934-5938 JJ denotes mean
T5091 5939-5940 NN denotes ±
T5092 5941-5944 NNP denotes SEM
T5093 5944-5945 -RRB- denotes )
T5094 5946-5951 NNS denotes years
T5095 5952-5953 -LRB- denotes (
T5096 5953-5958 NN denotes range
T5097 5959-5961 CD denotes 23
T5098 5962-5964 CD denotes 71
T5099 5965-5970 NNS denotes years
T5100 5970-5971 -RRB- denotes )
T5101 5971-5972 . denotes .
T5102 5973-5976 DT denotes All
T5103 5977-5988 NNS denotes medications
T5104 5989-5993 VBD denotes were
T5105 5994-6001 VBN denotes stopped
T5106 6002-6004 CD denotes 48
T5107 6005-6010 NNS denotes hours
T5108 6011-6017 IN denotes before
T5109 6018-6023 NN denotes entry
T5110 6024-6026 TO denotes to
T5111 6027-6030 DT denotes the
T5112 6031-6036 NN denotes study
T5113 6036-6037 . denotes .
T5114 6038-6049 NNS denotes Comparisons
T5115 6050-6054 VBD denotes were
T5116 6055-6059 VBN denotes made
T5117 6060-6064 IN denotes with
T5118 6065-6067 CD denotes 14
T5119 6068-6076 NNS denotes patients
T5120 6077-6081 IN denotes with
T5121 6082-6084 NNP denotes OA
T5122 6085-6086 -LRB- denotes (
T5123 6086-6087 CD denotes 3
T5124 6088-6091 NNS denotes men
T5125 6092-6095 CC denotes and
T5126 6096-6098 CD denotes 11
T5127 6099-6104 NNS denotes women
T5128 6104-6105 -RRB- denotes )
T5129 6106-6109 CC denotes and
T5130 6110-6114 IN denotes with
T5131 6115-6117 CD denotes 14
T5132 6118-6125 JJ denotes healthy
T5133 6126-6134 NNS denotes controls
T5134 6135-6136 -LRB- denotes (
T5135 6136-6137 CD denotes 3
T5136 6138-6141 NNS denotes men
T5137 6142-6145 CC denotes and
T5138 6146-6148 CD denotes 11
T5139 6149-6154 NNS denotes women
T5140 6154-6155 -RRB- denotes )
T5141 6156-6159 WP denotes who
T5142 6160-6163 VBD denotes had
T5143 6164-6166 DT denotes no
T5144 6167-6176 JJ denotes rheumatic
T5145 6177-6185 NNS denotes diseases
T5146 6185-6186 . denotes .
T5147 6187-6190 DT denotes The
T5148 6191-6195 JJ denotes mean
T5149 6196-6200 NNS denotes ages
T5150 6201-6203 IN denotes of
T5151 6204-6207 DT denotes the
T5152 6208-6216 NNS denotes patients
T5153 6217-6221 IN denotes with
T5154 6222-6224 NNP denotes OA
T5155 6225-6228 CC denotes and
T5156 6229-6232 DT denotes the
T5157 6233-6240 JJ denotes healthy
T5158 6241-6249 NNS denotes controls
T5159 6250-6254 VBD denotes were
T5160 6255-6257 CD denotes 50
T5161 6258-6259 NN denotes ±
T5162 6260-6261 CD denotes 8
T5163 6262-6267 NNS denotes years
T5164 6268-6269 -LRB- denotes (
T5165 6269-6274 NN denotes range
T5166 6275-6277 CD denotes 34
T5167 6278-6280 CD denotes 68
T5168 6281-6286 NNS denotes years
T5169 6286-6287 -RRB- denotes )
T5170 6288-6291 CC denotes and
T5171 6292-6294 CD denotes 30
T5172 6295-6296 NN denotes ±
T5173 6297-6298 CD denotes 6
T5174 6299-6304 NNS denotes years
T5175 6305-6306 -LRB- denotes (
T5176 6306-6311 NN denotes range
T5177 6312-6314 CD denotes 24
T5178 6315-6317 CD denotes 57
T5179 6318-6323 NNS denotes years
T5180 6323-6324 -RRB- denotes )
T5181 6324-6325 . denotes .
T5182 6326-6334 VBN denotes Informed
T5183 6335-6342 NN denotes consent
T5184 6343-6346 VBD denotes was
T5185 6347-6355 VBN denotes obtained
T5186 6355-6356 , denotes ,
T5187 6357-6360 CC denotes and
T5188 6361-6364 DT denotes the
T5189 6365-6373 NN denotes protocol
T5190 6374-6377 VBD denotes was
T5191 6378-6386 VBN denotes approved
T5192 6387-6389 IN denotes by
T5193 6390-6393 DT denotes the
T5194 6394-6402 NNP denotes Catholic
T5195 6403-6413 NNP denotes University
T5196 6414-6416 IN denotes of
T5197 6417-6422 NNP denotes Korea
T5198 6423-6428 NNP denotes Human
T5199 6429-6437 NNP denotes Research
T5200 6438-6444 NNP denotes Ethics
T5201 6445-6454 NNP denotes Committee
T5202 6454-6455 . denotes .
T5665 6457-6465 NNP denotes Reagents
T5666 6466-6477 NNP denotes Recombinant
T5667 6478-6483 NNP denotes IL-17
T5668 6483-6484 , denotes ,
T5669 6485-6490 NNP denotes IL-18
T5670 6490-6491 , denotes ,
T5671 6492-6497 NNP denotes IL-15
T5672 6497-6498 , denotes ,
T5673 6499-6507 NN denotes monocyte
T5674 6508-6523 NN denotes chemoattractant
T5675 6524-6533 NN denotes protein-1
T5676 6534-6535 -LRB- denotes (
T5677 6535-6540 NN denotes MCP-1
T5678 6540-6541 -RRB- denotes )
T5679 6541-6542 , denotes ,
T5680 6543-6553 NN denotes macrophage
T5681 6554-6566 JJ denotes inflammatory
T5682 6567-6574 NN denotes protein
T5683 6575-6576 -LRB- denotes (
T5684 6576-6579 NNP denotes MIP
T5685 6579-6580 -RRB- denotes )
T5686 6580-6582 CD denotes -1
T5687 6582-6583 NN denotes α
T5688 6583-6584 , denotes ,
T5689 6585-6591 NNP denotes MIP-1β
T5690 6591-6592 , denotes ,
T5691 6593-6597 NNP denotes IL-6
T5692 6598-6601 CC denotes and
T5693 6602-6606 NNP denotes IL-8
T5694 6607-6611 VBD denotes were
T5695 6612-6621 VBN denotes purchased
T5696 6622-6626 IN denotes from
T5697 6627-6628 NN denotes R
T5698 6629-6630 CC denotes &
T5699 6631-6632 NNP denotes D
T5700 6633-6640 NNS denotes systems
T5701 6641-6642 -LRB- denotes (
T5702 6642-6653 NNP denotes Minneapolis
T5703 6653-6654 , denotes ,
T5704 6655-6657 NNP denotes MN
T5705 6657-6658 , denotes ,
T5706 6659-6662 NNP denotes USA
T5707 6662-6663 -RRB- denotes )
T5708 6663-6664 . denotes .
T5709 6665-6676 JJ denotes Recombinant
T5710 6677-6689 VBG denotes transforming
T5711 6690-6696 NN denotes growth
T5712 6697-6703 NN denotes factor
T5713 6704-6705 -LRB- denotes (
T5714 6705-6708 NNP denotes TGF
T5715 6708-6709 -RRB- denotes )
T5716 6709-6710 : denotes -
T5717 6710-6711 NN denotes β
T5718 6712-6715 VBD denotes was
T5719 6716-6725 VBN denotes purchased
T5720 6726-6730 IN denotes from
T5721 6731-6740 NNP denotes Peprotech
T5722 6741-6742 -LRB- denotes (
T5723 6742-6748 NNP denotes London
T5724 6748-6749 , denotes ,
T5725 6750-6752 NNP denotes UK
T5726 6752-6753 -RRB- denotes )
T5727 6753-6754 . denotes .
T5728 6755-6766 JJ denotes Recombinant
T5729 6767-6772 NN denotes TNF-α
T5730 6773-6776 CC denotes and
T5731 6777-6781 NN denotes IL-1
T5732 6782-6786 VBD denotes were
T5733 6787-6796 VBN denotes purchased
T5734 6797-6801 IN denotes from
T5735 6802-6809 NNP denotes Endogen
T5736 6810-6814 NNP denotes Inc.
T5737 6815-6816 -LRB- denotes (
T5738 6816-6825 NNP denotes Cambridge
T5739 6825-6826 , denotes ,
T5740 6827-6829 NNP denotes MA
T5741 6829-6830 , denotes ,
T5742 6831-6834 NNP denotes USA
T5743 6834-6835 -RRB- denotes )
T5744 6835-6836 . denotes .
T5745 6837-6848 NNP denotes Cyclosporin
T5746 6849-6850 NNP denotes A
T5747 6851-6854 VBD denotes was
T5748 6855-6863 VBN denotes provided
T5749 6864-6866 IN denotes by
T5750 6867-6873 NNP denotes Sandos
T5751 6874-6878 NNP denotes Ltd.
T5752 6879-6880 -LRB- denotes (
T5753 6880-6885 NNP denotes Basel
T5754 6885-6886 , denotes ,
T5755 6887-6898 NNP denotes Switzerland
T5756 6898-6899 -RRB- denotes )
T5757 6899-6900 . denotes .
T5758 6901-6919 NNP denotes Phytohemagglutinin
T5759 6920-6921 -LRB- denotes (
T5760 6921-6924 NNP denotes PHA
T5761 6924-6925 -RRB- denotes )
T5762 6925-6926 , denotes ,
T5763 6927-6938 JJ denotes pyrrolidine
T5764 6939-6954 NN denotes dithiocarbamate
T5765 6955-6956 -LRB- denotes (
T5766 6956-6960 NNP denotes PDTC
T5767 6960-6961 -RRB- denotes )
T5768 6961-6962 , denotes ,
T5769 6963-6972 NN denotes rapamycin
T5770 6972-6973 , denotes ,
T5771 6974-6987 NN denotes dexamethasone
T5772 6988-6991 CC denotes and
T5773 6992-7000 NN denotes curcumin
T5774 7001-7005 VBD denotes were
T5775 7006-7009 DT denotes all
T5776 7010-7018 VBN denotes obtained
T5777 7019-7023 IN denotes from
T5778 7024-7027 DT denotes the
T5779 7028-7033 NNP denotes Sigma
T5780 7034-7042 NNP denotes Chemical
T5781 7043-7046 NNP denotes Co.
T5782 7047-7048 -LRB- denotes (
T5783 7048-7050 NNP denotes St
T5784 7051-7056 NNP denotes Louis
T5785 7056-7057 , denotes ,
T5786 7058-7060 NNP denotes MA
T5787 7060-7061 , denotes ,
T5788 7062-7065 NNP denotes USA
T5789 7065-7066 -RRB- denotes )
T5790 7066-7067 . denotes .
T5791 7068-7076 JJ denotes Anti-CD3
T5792 7077-7087 JJ denotes monoclonal
T5793 7088-7096 NN denotes antibody
T5794 7097-7100 CC denotes and
T5795 7101-7110 JJ denotes anti-CD28
T5796 7111-7121 JJ denotes monoclonal
T5797 7122-7130 NN denotes antibody
T5798 7131-7135 VBD denotes were
T5799 7136-7144 VBN denotes obtained
T5800 7145-7149 IN denotes from
T5801 7150-7152 NNP denotes BD
T5802 7153-7164 NNP denotes Biosciences
T5803 7165-7166 -LRB- denotes (
T5804 7166-7169 NNP denotes San
T5805 7170-7175 NNP denotes Diego
T5806 7175-7176 , denotes ,
T5807 7177-7179 NNP denotes CA
T5808 7179-7180 , denotes ,
T5809 7181-7184 NNP denotes USA
T5810 7184-7185 -RRB- denotes )
T5811 7185-7186 . denotes .
T5812 7187-7195 CD denotes LY294002
T5813 7195-7196 , denotes ,
T5814 7197-7205 NNP denotes SB203580
T5815 7205-7206 , denotes ,
T5816 7207-7212 NNP denotes FK506
T5817 7212-7213 , denotes ,
T5818 7214-7224 NN denotes wortmannin
T5819 7225-7228 CC denotes and
T5820 7229-7236 CD denotes PD98059
T5821 7237-7241 VBD denotes were
T5822 7242-7250 VBN denotes obtained
T5823 7251-7255 IN denotes from
T5824 7256-7266 NNP denotes Calbiochem
T5825 7267-7268 -LRB- denotes (
T5826 7268-7278 NNP denotes Schwalbach
T5827 7278-7279 , denotes ,
T5828 7280-7287 NNP denotes Germany
T5829 7287-7288 -RRB- denotes )
T5830 7288-7289 . denotes .
T6294 7291-7301 NN denotes Production
T6295 7302-7304 IN denotes of
T6296 7305-7310 NNP denotes IL-17
T6297 7311-7313 IN denotes by
T6298 7314-7315 NNP denotes T
T6299 7316-7320 NN denotes cell
T6300 7321-7329 NN denotes receptor
T6301 7330-7340 NN denotes activation
T6302 7340-7341 , denotes ,
T6303 7342-7351 NNS denotes cytokines
T6304 7352-7354 CC denotes or
T6305 7355-7365 NNS denotes chemokines
T6306 7366-7370 NNP denotes PBMC
T6307 7371-7375 VBD denotes were
T6308 7376-7384 VBN denotes prepared
T6309 7385-7389 IN denotes from
T6310 7390-7401 VBN denotes heparinized
T6311 7402-7407 NN denotes blood
T6312 7408-7410 IN denotes by
T6313 7411-7425 NNP denotes Ficoll-Hypaque
T6314 7426-7427 -LRB- denotes (
T6315 7427-7433 NNP denotes SG1077
T6316 7433-7434 -RRB- denotes )
T6317 7435-7451 JJ denotes density-gradient
T6318 7452-7466 NN denotes centrifugation
T6319 7466-7467 . denotes .
T6320 7468-7472 NNP denotes Cell
T6321 7473-7481 NNS denotes cultures
T6322 7482-7486 VBD denotes were
T6323 7487-7496 VBN denotes performed
T6324 7497-7499 IN denotes as
T6325 7500-7509 VBN denotes described
T6326 7510-7520 RB denotes previously
T6327 7521-7522 NNP denotes [
T6328 7522-7524 CD denotes 18
T6329 7524-7525 NNP denotes ]
T6330 7525-7526 . denotes .
T6331 7527-7529 IN denotes In
T6332 7530-7535 NN denotes brief
T6333 7535-7536 , denotes ,
T6334 7537-7540 DT denotes the
T6335 7541-7545 NN denotes cell
T6336 7546-7557 NNS denotes suspensions
T6337 7558-7562 VBD denotes were
T6338 7563-7571 VBN denotes adjusted
T6339 7572-7574 TO denotes to
T6340 7575-7576 DT denotes a
T6341 7577-7590 NN denotes concentration
T6342 7591-7593 IN denotes of
T6343 7594-7600 CD denotes 106/ml
T6344 7601-7603 IN denotes in
T6345 7604-7608 NNP denotes RPMI
T6346 7609-7613 CD denotes 1640
T6347 7614-7620 JJ denotes medium
T6348 7621-7633 VBN denotes supplemented
T6349 7634-7638 IN denotes with
T6350 7639-7641 CD denotes 10
T6351 7641-7642 NN denotes %
T6352 7643-7648 JJ denotes fetal
T6353 7649-7653 NN denotes calf
T6354 7654-7659 NN denotes serum
T6355 7659-7660 , denotes ,
T6356 7661-7664 CD denotes 100
T6357 7665-7669 NNP denotes U/ml
T6358 7670-7680 NN denotes penicillin
T6359 7680-7681 , denotes ,
T6360 7682-7685 CD denotes 100
T6361 7686-7691 JJ denotes mg/ml
T6362 7692-7704 NN denotes streptomycin
T6363 7705-7708 CC denotes and
T6364 7709-7710 CD denotes 2
T6365 7711-7713 NNP denotes mM
T6366 7714-7725 NNP denotes L-glutamine
T6367 7725-7726 . denotes .
T6368 7727-7731 NNP denotes Cell
T6369 7732-7742 NN denotes suspension
T6370 7743-7744 -LRB- denotes (
T6371 7744-7745 CD denotes 1
T6372 7746-7748 NN denotes ml
T6373 7748-7749 -RRB- denotes )
T6374 7750-7753 VBD denotes was
T6375 7754-7763 VBN denotes dispensed
T6376 7764-7768 IN denotes into
T6377 7769-7776 JJ denotes 24-well
T6378 7777-7787 JJ denotes multi-well
T6379 7788-7794 NNS denotes plates
T6380 7795-7796 -LRB- denotes (
T6381 7796-7800 NNP denotes Nunc
T6382 7800-7801 , denotes ,
T6383 7802-7810 NNP denotes Roskilde
T6384 7810-7811 , denotes ,
T6385 7812-7819 NNP denotes Denmark
T6386 7819-7820 -RRB- denotes )
T6387 7820-7821 , denotes ,
T6388 7822-7825 CC denotes and
T6389 7826-7835 VBD denotes incubated
T6390 7836-7839 IN denotes for
T6391 7840-7842 CD denotes 24
T6392 7843-7848 NNS denotes hours
T6393 7849-7851 IN denotes at
T6394 7852-7854 CD denotes 37
T6395 7854-7855 CD denotes °
T6396 7855-7856 NNP denotes C
T6397 7857-7859 IN denotes in
T6398 7860-7861 CD denotes 5
T6399 7861-7862 NN denotes %
T6400 7863-7866 CD denotes CO2
T6401 7866-7867 . denotes .
T6402 7868-7880 RB denotes Subsequently
T6403 7880-7881 , denotes ,
T6404 7882-7889 JJ denotes various
T6405 7890-7904 NNS denotes concentrations
T6406 7905-7907 IN denotes of
T6407 7908-7919 NN denotes cyclosporin
T6408 7920-7921 DT denotes A
T6409 7922-7923 -LRB- denotes (
T6410 7923-7925 CD denotes 10
T6411 7926-7929 CD denotes 500
T6412 7930-7935 NN denotes ng/ml
T6413 7935-7936 -RRB- denotes )
T6414 7937-7941 VBD denotes were
T6415 7942-7947 VBN denotes added
T6416 7948-7950 TO denotes to
T6417 7951-7954 DT denotes the
T6418 7955-7961 NN denotes medium
T6419 7962-7965 CC denotes and
T6420 7966-7971 NNS denotes cells
T6421 7972-7976 VBD denotes were
T6422 7977-7986 VBN denotes incubated
T6423 7987-7990 IN denotes for
T6424 7991-7993 CD denotes 24
T6425 7994-7999 NNS denotes hours
T6426 7999-8000 . denotes .
T6427 8001-8003 TO denotes To
T6428 8004-8008 DT denotes each
T6429 8009-8013 NN denotes well
T6430 8014-8017 VBD denotes was
T6431 8018-8023 VBN denotes added
T6432 8024-8029 NNP denotes FK506
T6433 8029-8030 , denotes ,
T6434 8031-8040 NN denotes rapamycin
T6435 8040-8041 , denotes ,
T6436 8042-8050 NN denotes curcumin
T6437 8050-8051 , denotes ,
T6438 8052-8056 NNP denotes PDTC
T6439 8056-8057 , denotes ,
T6440 8058-8066 NNP denotes LY294002
T6441 8066-8067 , denotes ,
T6442 8068-8076 NNP denotes SB203580
T6443 8076-8077 , denotes ,
T6444 8078-8085 NNP denotes PD98059
T6445 8085-8086 , denotes ,
T6446 8087-8100 NN denotes dexamethasone
T6447 8101-8103 CC denotes or
T6448 8104-8114 NN denotes wortmannin
T6449 8114-8115 . denotes .
T6450 8116-8121 IN denotes After
T6451 8122-8132 NN denotes incubation
T6452 8133-8136 IN denotes for
T6453 8137-8139 CD denotes 24
T6454 8140-8145 NNS denotes hours
T6455 8146-8147 -LRB- denotes (
T6456 8147-8153 IN denotes unless
T6457 8154-8163 RB denotes otherwise
T6458 8164-8170 VBN denotes stated
T6459 8170-8171 -RRB- denotes )
T6460 8171-8172 , denotes ,
T6461 8173-8182 JJ denotes cell-free
T6462 8183-8188 NNS denotes media
T6463 8189-8193 VBD denotes were
T6464 8194-8203 VBN denotes collected
T6465 8204-8207 CC denotes and
T6466 8208-8214 VBN denotes stored
T6467 8215-8217 IN denotes at
T6468 8218-8221 CD denotes -20
T6469 8221-8222 CD denotes °
T6470 8222-8223 NNP denotes C
T6471 8224-8229 IN denotes until
T6472 8230-8237 VBN denotes assayed
T6473 8237-8238 . denotes .
T6474 8239-8242 DT denotes All
T6475 8243-8251 NNS denotes cultures
T6476 8252-8256 VBD denotes were
T6477 8257-8260 VBN denotes set
T6478 8261-8263 RP denotes up
T6479 8264-8266 IN denotes in
T6480 8267-8277 NN denotes triplicate
T6481 8277-8278 , denotes ,
T6482 8279-8282 CC denotes and
T6483 8283-8290 NNS denotes results
T6484 8291-8294 VBP denotes are
T6485 8295-8304 VBN denotes expressed
T6486 8305-8307 IN denotes as
T6487 8308-8313 NNS denotes means
T6488 8314-8315 VBP denotes ±
T6489 8316-8319 NNP denotes SEM
T6490 8319-8320 . denotes .
T6978 8322-8325 CD denotes CD4
T6979 8325-8326 CD denotes +
T6980 8327-8333 NNP denotes T-cell
T6981 8334-8343 NN denotes isolation
T6982 8344-8346 IN denotes by
T6983 8347-8351 NNP denotes MACS
T6984 8352-8360 JJ denotes Anti-CD4
T6985 8361-8371 NNS denotes microbeads
T6986 8372-8376 VBD denotes were
T6987 8377-8381 VBN denotes used
T6988 8382-8393 RB denotes essentially
T6989 8394-8396 IN denotes as
T6990 8397-8408 VBN denotes recommended
T6991 8409-8411 IN denotes by
T6992 8412-8415 DT denotes the
T6993 8416-8428 NN denotes manufacturer
T6994 8429-8430 -LRB- denotes (
T6995 8430-8438 NNP denotes Miltenyi
T6996 8438-8439 -RRB- denotes )
T6997 8440-8441 NNP denotes [
T6998 8441-8443 CD denotes 19
T6999 8443-8444 NNP denotes ]
T7000 8444-8445 . denotes .
T7001 8446-8450 NNP denotes PBMC
T7002 8451-8455 VBD denotes were
T7003 8456-8467 VBN denotes resuspended
T7004 8468-8470 IN denotes in
T7005 8471-8473 CD denotes 80
T7006 8474-8476 NN denotes μl
T7007 8477-8479 IN denotes of
T7008 8480-8483 NNP denotes FBS
T7009 8484-8492 VBG denotes staining
T7010 8493-8499 NN denotes buffer
T7011 8499-8500 . denotes .
T7012 8501-8509 JJ denotes Anti-CD4
T7013 8510-8520 NNS denotes microbeads
T7014 8521-8522 -LRB- denotes (
T7015 8522-8524 CD denotes 20
T7016 8525-8527 NN denotes μl
T7017 8527-8528 -RRB- denotes )
T7018 8529-8533 VBD denotes were
T7019 8534-8539 VBN denotes added
T7020 8540-8543 CC denotes and
T7021 8544-8553 VBN denotes incubated
T7022 8554-8557 IN denotes for
T7023 8558-8560 CD denotes 15
T7024 8561-8564 NN denotes min
T7025 8565-8567 IN denotes at
T7026 8568-8569 CD denotes 6
T7027 8570-8572 CD denotes 12
T7028 8572-8573 NN denotes °
T7029 8573-8575 NNP denotes C.
T7030 8576-8586 NNP denotes Saturating
T7031 8587-8594 NNS denotes amounts
T7032 8595-8597 IN denotes of
T7033 8598-8621 JJ denotes fluorochrome-conjugated
T7034 8622-8632 NNS denotes antibodies
T7035 8633-8637 VBD denotes were
T7036 8638-8643 VBN denotes added
T7037 8644-8647 IN denotes for
T7038 8648-8649 DT denotes a
T7039 8650-8657 JJ denotes further
T7040 8658-8660 CD denotes 10
T7041 8661-8664 NN denotes min
T7042 8664-8665 . denotes .
T7043 8666-8671 NNS denotes Cells
T7044 8672-8676 VBD denotes were
T7045 8677-8684 VBN denotes diluted
T7046 8685-8687 IN denotes in
T7047 8688-8691 CD denotes 2.5
T7048 8692-8694 NN denotes ml
T7049 8695-8697 IN denotes of
T7050 8698-8701 NNP denotes FBS
T7051 8702-8710 VBG denotes staining
T7052 8711-8717 NN denotes buffer
T7053 8717-8718 , denotes ,
T7054 8719-8727 VBN denotes pelleted
T7055 8727-8728 , denotes ,
T7056 8729-8740 VBN denotes resuspended
T7057 8741-8743 IN denotes in
T7058 8744-8747 CD denotes 500
T7059 8748-8750 NN denotes μl
T7060 8751-8754 CC denotes and
T7061 8755-8767 RB denotes magnetically
T7062 8768-8777 VBD denotes separated
T7063 8777-8778 , denotes ,
T7064 8779-8786 RB denotes usually
T7065 8787-8789 IN denotes on
T7066 8790-8792 DT denotes an
T7067 8793-8801 NNP denotes AutoMACS
T7068 8802-8808 NN denotes magnet
T7069 8809-8815 VBN denotes fitted
T7070 8816-8820 IN denotes with
T7071 8821-8822 DT denotes a
T7072 8823-8827 NNP denotes MACS
T7073 8828-8830 NNP denotes MS
T7074 8831-8837 NN denotes column
T7075 8837-8838 . denotes .
T7076 8839-8851 JJ denotes Flow-through
T7077 8852-8855 CC denotes and
T7078 8856-8859 CD denotes two
T7079 8860-8861 CD denotes 1
T7080 8862-8864 NN denotes ml
T7081 8865-8871 NNS denotes washes
T7082 8872-8876 VBD denotes were
T7083 8877-8886 VBN denotes collected
T7084 8887-8889 IN denotes as
T7085 8890-8893 DT denotes the
T7086 8894-8902 JJ denotes negative
T7087 8903-8911 NN denotes fraction
T7088 8911-8912 . denotes .
T7089 8913-8921 JJ denotes Enriched
T7090 8922-8927 NNS denotes cells
T7091 8928-8932 VBD denotes were
T7092 8933-8942 VBN denotes collected
T7093 8943-8945 IN denotes in
T7094 8946-8949 CD denotes two
T7095 8950-8953 CD denotes 0.5
T7096 8954-8956 NN denotes ml
T7097 8957-8965 NNS denotes aliquots
T7098 8966-8970 IN denotes from
T7099 8971-8974 DT denotes the
T7100 8975-8981 NN denotes column
T7101 8982-8987 IN denotes after
T7102 8988-8995 NN denotes removal
T7103 8996-9000 IN denotes from
T7104 9001-9004 DT denotes the
T7105 9005-9011 NN denotes magnet
T7106 9011-9012 . denotes .
T7107 9013-9026 RB denotes Alternatively
T7108 9026-9027 , denotes ,
T7109 9028-9033 NNS denotes cells
T7110 9034-9041 VBD denotes stained
T7111 9042-9046 IN denotes with
T7112 9047-9055 JJ denotes anti-CD4
T7113 9056-9069 NN denotes phycoerythrin
T7114 9070-9074 VBD denotes were
T7115 9075-9081 VBN denotes washed
T7116 9081-9082 , denotes ,
T7117 9083-9095 RB denotes magnetically
T7118 9096-9103 VBN denotes labeled
T7119 9104-9108 IN denotes with
T7120 9109-9127 JJ denotes anti-phycoerythrin
T7121 9128-9138 NNS denotes microbeads
T7122 9139-9140 -LRB- denotes (
T7123 9140-9142 CD denotes 20
T7124 9143-9145 NN denotes μl
T7125 9146-9151 VBD denotes added
T7126 9152-9154 TO denotes to
T7127 9155-9157 CD denotes 80
T7128 9158-9160 NN denotes μl
T7129 9161-9163 IN denotes of
T7130 9164-9168 NN denotes cell
T7131 9169-9179 NN denotes suspension
T7132 9179-9180 : denotes ;
T7133 9181-9183 CD denotes 15
T7134 9184-9187 NN denotes min
T7135 9187-9188 , denotes ,
T7136 9189-9190 CD denotes 6
T7137 9191-9193 CD denotes 12
T7138 9193-9194 NN denotes °
T7139 9194-9195 NNP denotes C
T7140 9195-9196 -RRB- denotes )
T7141 9196-9197 , denotes ,
T7142 9198-9201 CC denotes and
T7143 9202-9214 RB denotes magnetically
T7144 9215-9224 VBD denotes separated
T7145 9225-9227 IN denotes as
T7146 9228-9237 VBN denotes described
T7147 9238-9243 IN denotes above
T7148 9243-9244 . denotes .
T7149 9245-9248 DT denotes The
T7150 9249-9255 NN denotes purity
T7151 9256-9258 IN denotes of
T7152 9259-9264 NNS denotes cells
T7153 9265-9268 VBD denotes was
T7154 9269-9277 VBN denotes assessed
T7155 9278-9280 IN denotes by
T7156 9281-9285 NN denotes flow
T7157 9286-9296 JJ denotes cytometric
T7158 9297-9305 NN denotes analysis
T7159 9306-9308 IN denotes of
T7160 9309-9316 JJ denotes stained
T7161 9317-9322 NNS denotes cells
T7162 9323-9325 IN denotes on
T7163 9326-9327 DT denotes a
T7164 9328-9332 NNP denotes FACS
T7165 9333-9340 NNP denotes Vantage
T7166 9341-9347 NN denotes sorter
T7167 9347-9348 . denotes .
T7168 9349-9353 JJS denotes Most
T7169 9354-9355 -LRB- denotes (
T7170 9355-9359 JJR denotes more
T7171 9360-9364 IN denotes than
T7172 9365-9367 CD denotes 97
T7173 9367-9368 NN denotes %
T7174 9368-9369 -RRB- denotes )
T7175 9370-9372 IN denotes of
T7176 9373-9376 DT denotes the
T7177 9377-9385 JJ denotes isolated
T7178 9386-9391 NNS denotes cells
T7179 9392-9395 VBD denotes had
T7180 9396-9399 DT denotes the
T7181 9400-9403 NNP denotes CD4
T7182 9404-9405 NNP denotes T
T7183 9406-9410 NN denotes cell
T7184 9411-9417 NN denotes marker
T7185 9417-9418 . denotes .
T7843 9420-9433 JJ denotes Enzyme-linked
T7844 9434-9447 JJ denotes immunosorbent
T7845 9448-9453 NN denotes assay
T7846 9454-9456 IN denotes of
T7847 9457-9462 NNP denotes IL-17
T7848 9463-9468 NNP denotes IL-17
T7849 9469-9471 IN denotes in
T7850 9472-9479 NN denotes culture
T7851 9480-9492 NNS denotes supernatants
T7852 9493-9496 VBD denotes was
T7853 9497-9505 VBN denotes measured
T7854 9506-9508 IN denotes by
T7855 9509-9517 NN denotes sandwich
T7856 9518-9531 JJ denotes enzyme-linked
T7857 9532-9545 JJ denotes immunosorbent
T7858 9546-9551 NN denotes assay
T7859 9552-9554 IN denotes as
T7860 9555-9564 VBN denotes described
T7861 9565-9575 RB denotes previously
T7862 9576-9577 NNP denotes [
T7863 9577-9579 CD denotes 20
T7864 9579-9580 NNP denotes ]
T7865 9580-9581 . denotes .
T7866 9582-9584 IN denotes In
T7867 9585-9590 NN denotes brief
T7868 9590-9591 , denotes ,
T7869 9592-9593 DT denotes a
T7870 9594-9601 JJ denotes 96-well
T7871 9602-9607 NN denotes plate
T7872 9608-9609 -LRB- denotes (
T7873 9609-9613 NNP denotes Nunc
T7874 9613-9614 -RRB- denotes )
T7875 9615-9618 VBD denotes was
T7876 9619-9625 VBN denotes coated
T7877 9626-9630 IN denotes with
T7878 9631-9632 CD denotes 4
T7879 9633-9635 NN denotes μg
T7880 9635-9636 NN denotes /
T7881 9636-9638 NN denotes ml
T7882 9639-9649 JJ denotes monoclonal
T7883 9650-9660 NNS denotes antibodies
T7884 9661-9668 IN denotes against
T7885 9669-9674 NNP denotes IL-17
T7886 9675-9676 -LRB- denotes (
T7887 9676-9677 NNP denotes R
T7888 9678-9679 CC denotes &
T7889 9680-9681 NNP denotes D
T7890 9682-9689 NNPS denotes Systems
T7891 9689-9690 -RRB- denotes )
T7892 9691-9693 IN denotes at
T7893 9694-9695 CD denotes 4
T7894 9695-9696 CD denotes °
T7895 9696-9697 NNP denotes C
T7896 9698-9707 JJ denotes overnight
T7897 9707-9708 . denotes .
T7898 9709-9714 IN denotes After
T7899 9715-9723 VBG denotes blocking
T7900 9724-9728 IN denotes with
T7901 9729-9747 JJ denotes phosphate-buffered
T7902 9748-9756 CD denotes saline/1
T7903 9756-9757 NN denotes %
T7904 9758-9764 JJ denotes bovine
T7905 9765-9770 NN denotes serum
T7906 9771-9778 NN denotes albumin
T7907 9779-9780 -LRB- denotes (
T7908 9780-9783 NNP denotes BSA
T7909 9783-9784 -RRB- denotes )
T7910 9784-9785 VBP denotes /
T7911 9785-9789 CD denotes 0.05
T7912 9789-9790 NN denotes %
T7913 9791-9796 CD denotes Tween
T7914 9797-9799 CD denotes 20
T7915 9800-9803 IN denotes for
T7916 9804-9805 CD denotes 2
T7917 9806-9811 NNS denotes hours
T7918 9812-9814 IN denotes at
T7919 9815-9819 NN denotes room
T7920 9820-9831 NN denotes temperature
T7921 9832-9833 -LRB- denotes (
T7922 9833-9835 CD denotes 22
T7923 9836-9838 CD denotes 25
T7924 9838-9839 NN denotes °
T7925 9839-9840 NNP denotes C
T7926 9840-9841 -RRB- denotes )
T7927 9841-9842 , denotes ,
T7928 9843-9847 NN denotes test
T7929 9848-9855 NNS denotes samples
T7930 9856-9859 CC denotes and
T7931 9860-9863 DT denotes the
T7932 9864-9872 JJ denotes standard
T7933 9873-9884 JJ denotes recombinant
T7934 9885-9890 NNP denotes IL-17
T7935 9891-9892 -LRB- denotes (
T7936 9892-9893 NNP denotes R
T7937 9894-9895 CC denotes &
T7938 9896-9897 NNP denotes D
T7939 9898-9905 NNPS denotes Systems
T7940 9905-9906 -RRB- denotes )
T7941 9907-9911 VBD denotes were
T7942 9912-9917 VBN denotes added
T7943 9918-9920 TO denotes to
T7944 9921-9924 DT denotes the
T7945 9925-9932 JJ denotes 96-well
T7946 9933-9938 NN denotes plate
T7947 9939-9942 CC denotes and
T7948 9943-9952 VBD denotes incubated
T7949 9953-9955 IN denotes at
T7950 9956-9960 NN denotes room
T7951 9961-9972 NN denotes temperature
T7952 9973-9976 IN denotes for
T7953 9977-9978 CD denotes 2
T7954 9979-9984 NNS denotes hours
T7955 9984-9985 . denotes .
T7956 9986-9992 NNS denotes Plates
T7957 9993-9997 VBD denotes were
T7958 9998-10004 VBN denotes washed
T7959 10005-10009 CD denotes four
T7960 10010-10015 NNS denotes times
T7961 10016-10020 IN denotes with
T7962 10021-10039 JJ denotes phosphate-buffered
T7963 10040-10052 NNP denotes saline/Tween
T7964 10053-10055 CD denotes 20
T7965 10055-10056 , denotes ,
T7966 10057-10060 CC denotes and
T7967 10061-10065 RB denotes then
T7968 10066-10075 VBN denotes incubated
T7969 10076-10080 IN denotes with
T7970 10081-10084 CD denotes 500
T7971 10085-10090 JJ denotes ng/ml
T7972 10091-10103 VBN denotes biotinylated
T7973 10104-10109 NN denotes mouse
T7974 10110-10120 NN denotes monoclonal
T7975 10121-10131 NNS denotes antibodies
T7976 10132-10139 IN denotes against
T7977 10140-10145 NNP denotes IL-17
T7978 10146-10147 -LRB- denotes (
T7979 10147-10148 NNP denotes R
T7980 10149-10150 CC denotes &
T7981 10151-10152 NNP denotes D
T7982 10153-10160 NNPS denotes Systems
T7983 10160-10161 -RRB- denotes )
T7984 10162-10165 IN denotes for
T7985 10166-10167 CD denotes 2
T7986 10168-10173 NNS denotes hours
T7987 10174-10176 IN denotes at
T7988 10177-10181 NN denotes room
T7989 10182-10193 NN denotes temperature
T7990 10193-10194 . denotes .
T7991 10195-10200 IN denotes After
T7992 10201-10208 VBG denotes washing
T7993 10208-10209 , denotes ,
T7994 10210-10222 NNP denotes streptavidin
T7995 10223-10231 JJ denotes alkaline
T7996 10232-10241 NN denotes phosphate
T7997 10242-10253 JJ denotes horseradish
T7998 10254-10264 JJ denotes peroxidase
T7999 10265-10274 NN denotes conjugate
T8000 10275-10276 -LRB- denotes (
T8001 10276-10281 NNP denotes Sigma
T8002 10281-10282 -RRB- denotes )
T8003 10283-10286 VBD denotes was
T8004 10287-10296 VBN denotes incubated
T8005 10297-10300 IN denotes for
T8006 10301-10302 CD denotes 2
T8007 10303-10308 NNS denotes hours
T8008 10308-10309 , denotes ,
T8009 10310-10314 RB denotes then
T8010 10315-10321 VBD denotes washed
T8011 10322-10327 RB denotes again
T8012 10328-10331 CC denotes and
T8013 10332-10341 VBN denotes incubated
T8014 10342-10346 IN denotes with
T8015 10347-10348 CD denotes 1
T8016 10349-10354 NN denotes mg/ml
T8017 10355-10368 NN denotes p-nitrophenyl
T8018 10369-10378 NN denotes phosphate
T8019 10379-10380 -LRB- denotes (
T8020 10380-10385 NNP denotes Sigma
T8021 10385-10386 -RRB- denotes )
T8022 10387-10396 VBN denotes dissolved
T8023 10397-10399 IN denotes in
T8024 10400-10414 NN denotes diethanolamine
T8025 10415-10416 -LRB- denotes (
T8026 10416-10421 NNP denotes Sigma
T8027 10421-10422 -RRB- denotes )
T8028 10423-10425 TO denotes to
T8029 10426-10433 VB denotes develop
T8030 10434-10437 DT denotes the
T8031 10438-10443 NN denotes color
T8032 10444-10452 NN denotes reaction
T8033 10452-10453 . denotes .
T8034 10454-10457 DT denotes The
T8035 10458-10466 NN denotes reaction
T8036 10467-10470 VBD denotes was
T8037 10471-10478 VBN denotes stopped
T8038 10479-10481 IN denotes by
T8039 10482-10485 DT denotes the
T8040 10486-10494 NN denotes addition
T8041 10495-10497 IN denotes of
T8042 10498-10499 CD denotes 1
T8043 10500-10501 NNP denotes M
T8044 10502-10506 NNP denotes NaOH
T8045 10507-10510 CC denotes and
T8046 10511-10514 DT denotes the
T8047 10515-10522 JJ denotes optical
T8048 10523-10530 NN denotes density
T8049 10531-10533 IN denotes of
T8050 10534-10538 DT denotes each
T8051 10539-10543 NN denotes well
T8052 10544-10547 VBD denotes was
T8053 10548-10552 VBN denotes read
T8054 10553-10555 IN denotes at
T8055 10556-10559 CD denotes 405
T8056 10560-10562 NN denotes nm
T8057 10562-10563 . denotes .
T8058 10564-10567 DT denotes The
T8059 10568-10573 JJR denotes lower
T8060 10574-10579 NN denotes limit
T8061 10580-10582 IN denotes of
T8062 10583-10588 NNP denotes IL-17
T8063 10589-10598 NN denotes detection
T8064 10599-10602 VBD denotes was
T8065 10603-10605 CD denotes 10
T8066 10606-10611 NN denotes pg/ml
T8067 10611-10612 . denotes .
T8068 10613-10624 JJ denotes Recombinant
T8069 10625-10630 JJ denotes human
T8070 10631-10636 NN denotes IL-17
T8071 10637-10644 VBN denotes diluted
T8072 10645-10647 IN denotes in
T8073 10648-10655 NN denotes culture
T8074 10656-10662 NN denotes medium
T8075 10663-10666 VBD denotes was
T8076 10667-10671 VBN denotes used
T8077 10672-10674 IN denotes as
T8078 10675-10676 DT denotes a
T8079 10677-10688 NN denotes calibration
T8080 10689-10697 NN denotes standard
T8081 10697-10698 , denotes ,
T8082 10699-10706 VBG denotes ranging
T8083 10707-10711 IN denotes from
T8084 10712-10714 CD denotes 10
T8085 10715-10717 TO denotes to
T8086 10718-10722 CD denotes 2000
T8087 10723-10728 NN denotes pg/ml
T8088 10728-10729 . denotes .
T8089 10730-10731 DT denotes A
T8090 10732-10740 JJ denotes standard
T8091 10741-10746 NN denotes curve
T8092 10747-10750 VBD denotes was
T8093 10751-10756 VBN denotes drawn
T8094 10757-10759 IN denotes by
T8095 10760-10768 VBG denotes plotting
T8096 10769-10776 JJ denotes optical
T8097 10777-10784 NN denotes density
T8098 10785-10792 IN denotes against
T8099 10793-10796 DT denotes the
T8100 10797-10800 VB denotes log
T8101 10801-10803 IN denotes of
T8102 10804-10807 DT denotes the
T8103 10808-10821 NN denotes concentration
T8104 10822-10824 IN denotes of
T8105 10825-10836 JJ denotes recombinant
T8106 10837-10846 NNS denotes cytokines
T8107 10846-10847 , denotes ,
T8108 10848-10851 CC denotes and
T8109 10852-10856 VBN denotes used
T8110 10857-10860 IN denotes for
T8111 10861-10874 NN denotes determination
T8112 10875-10877 IN denotes of
T8113 10878-10883 NNP denotes IL-17
T8114 10884-10886 IN denotes in
T8115 10887-10891 NN denotes test
T8116 10892-10899 NNS denotes samples
T8117 10899-10900 . denotes .
T8766 10902-10916 NN denotes Quantification
T8767 10917-10919 IN denotes of
T8768 10920-10925 NNP denotes IL-17
T8769 10926-10930 NNP denotes mRNA
T8770 10931-10933 IN denotes by
T8771 10934-10950 JJ denotes semiquantitative
T8772 10951-10958 NN denotes reverse
T8773 10959-10972 NN denotes transcription
T8774 10973-10983 NN denotes polymerase
T8775 10984-10989 NN denotes chain
T8776 10990-10998 NN denotes reaction
T8777 10999-11003 NNP denotes PBMC
T8778 11004-11008 VBD denotes were
T8779 11009-11018 VBN denotes incubated
T8780 11019-11023 IN denotes with
T8781 11024-11031 JJ denotes various
T8782 11032-11046 NNS denotes concentrations
T8783 11047-11049 IN denotes of
T8784 11050-11058 JJ denotes anti-CD3
T8785 11059-11061 IN denotes in
T8786 11062-11065 DT denotes the
T8787 11066-11074 NN denotes presence
T8788 11075-11077 CC denotes or
T8789 11078-11085 NN denotes absence
T8790 11086-11088 IN denotes of
T8791 11089-11099 NNS denotes inhibitors
T8792 11100-11101 -LRB- denotes (
T8793 11101-11109 CD denotes LY294002
T8794 11109-11110 , denotes ,
T8795 11111-11115 NNP denotes PDTC
T8796 11115-11116 -RRB- denotes )
T8797 11116-11117 . denotes .
T8798 11118-11123 IN denotes After
T8799 11124-11126 CD denotes 16
T8800 11127-11132 NNS denotes hours
T8801 11133-11135 IN denotes of
T8802 11136-11146 NN denotes incubation
T8803 11146-11147 , denotes ,
T8804 11148-11152 NNP denotes mRNA
T8805 11153-11156 VBD denotes was
T8806 11157-11166 VBN denotes extracted
T8807 11167-11171 IN denotes with
T8808 11172-11178 NNP denotes RNAzol
T8809 11179-11180 NNP denotes B
T8810 11181-11182 -LRB- denotes (
T8811 11182-11188 NNP denotes Biotex
T8812 11189-11201 NNPS denotes Laboratories
T8813 11201-11202 , denotes ,
T8814 11203-11210 NNP denotes Houston
T8815 11210-11211 , denotes ,
T8816 11212-11214 NNP denotes TX
T8817 11214-11215 , denotes ,
T8818 11216-11219 NNP denotes USA
T8819 11219-11220 -RRB- denotes )
T8820 11221-11223 IN denotes in
T8821 11224-11234 NN denotes accordance
T8822 11235-11239 IN denotes with
T8823 11240-11243 DT denotes the
T8824 11244-11256 NN denotes manufacturer
T8825 11256-11258 POS denotes 's
T8826 11259-11271 NNS denotes instructions
T8827 11271-11272 . denotes .
T8828 11273-11280 VB denotes Reverse
T8829 11281-11294 NN denotes transcription
T8830 11295-11297 IN denotes of
T8831 11298-11299 CD denotes 2
T8832 11300-11302 NN denotes μg
T8833 11303-11305 IN denotes of
T8834 11306-11311 JJ denotes total
T8835 11312-11316 NNP denotes mRNA
T8836 11317-11320 VBD denotes was
T8837 11321-11330 VBN denotes performed
T8838 11331-11333 IN denotes at
T8839 11334-11336 CD denotes 42
T8840 11336-11337 CD denotes °
T8841 11337-11338 NNP denotes C
T8842 11339-11344 VBG denotes using
T8843 11345-11348 DT denotes the
T8844 11349-11360 NNP denotes Superscript
T8845 11360-11361 NN denotes ™
T8846 11362-11369 NN denotes reverse
T8847 11370-11383 NN denotes transcription
T8848 11384-11390 NN denotes system
T8849 11391-11392 -LRB- denotes (
T8850 11392-11398 NNP denotes Takara
T8851 11398-11399 , denotes ,
T8852 11400-11405 NNP denotes Shiga
T8853 11405-11406 , denotes ,
T8854 11407-11412 NNP denotes Japan
T8855 11412-11413 -RRB- denotes )
T8856 11413-11414 . denotes .
T8857 11415-11418 NNP denotes PCR
T8858 11419-11432 NN denotes amplification
T8859 11433-11435 IN denotes of
T8860 11436-11440 NNP denotes cDNA
T8861 11441-11449 NNS denotes aliquots
T8862 11450-11453 VBD denotes was
T8863 11454-11463 VBN denotes performed
T8864 11464-11466 IN denotes by
T8865 11467-11473 VBG denotes adding
T8866 11474-11477 CD denotes 2.5
T8867 11478-11480 NNP denotes mM
T8868 11481-11486 NNP denotes dNTPs
T8869 11486-11487 , denotes ,
T8870 11488-11491 CD denotes 2.5
T8871 11492-11493 NNP denotes U
T8872 11494-11496 IN denotes of
T8873 11497-11500 NNP denotes Taq
T8874 11501-11504 NNP denotes DNA
T8875 11505-11515 NN denotes polymerase
T8876 11516-11517 -LRB- denotes (
T8877 11517-11523 NNP denotes Takara
T8878 11523-11524 -RRB- denotes )
T8879 11525-11528 CC denotes and
T8880 11529-11533 CD denotes 0.25
T8881 11534-11536 NNP denotes μM
T8882 11537-11539 IN denotes of
T8883 11540-11545 NN denotes sense
T8884 11546-11549 CC denotes and
T8885 11550-11559 JJ denotes antisense
T8886 11560-11567 NNS denotes primers
T8887 11567-11568 . denotes .
T8888 11569-11572 DT denotes The
T8889 11573-11581 NN denotes reaction
T8890 11582-11585 VBD denotes was
T8891 11586-11595 VBN denotes performed
T8892 11596-11598 IN denotes in
T8893 11599-11602 NNP denotes PCR
T8894 11603-11609 NN denotes buffer
T8895 11610-11611 -LRB- denotes (
T8896 11611-11614 CD denotes 1.5
T8897 11615-11617 NNP denotes mM
T8898 11618-11623 NNP denotes MgCl2
T8899 11623-11624 , denotes ,
T8900 11625-11627 CD denotes 50
T8901 11628-11630 NNP denotes mM
T8902 11631-11634 NNP denotes KCl
T8903 11634-11635 , denotes ,
T8904 11636-11638 CD denotes 10
T8905 11639-11641 NNP denotes mM
T8906 11642-11650 NNP denotes Tris-HCl
T8907 11650-11651 , denotes ,
T8908 11652-11654 NNP denotes pH
T8909 11655-11658 CD denotes 8.3
T8910 11658-11659 -RRB- denotes )
T8911 11660-11662 IN denotes in
T8912 11663-11664 DT denotes a
T8913 11665-11670 JJ denotes total
T8914 11671-11677 NN denotes volume
T8915 11678-11680 IN denotes of
T8916 11681-11683 CD denotes 25
T8917 11684-11686 NN denotes μl
T8918 11686-11687 . denotes .
T8919 11688-11691 DT denotes The
T8920 11692-11701 JJ denotes following
T8921 11702-11707 NN denotes sense
T8922 11708-11711 CC denotes and
T8923 11712-11721 JJ denotes antisense
T8924 11722-11729 NNS denotes primers
T8925 11730-11733 IN denotes for
T8926 11734-11738 DT denotes each
T8927 11739-11748 NNS denotes molecules
T8928 11749-11753 VBD denotes were
T8929 11754-11758 VBN denotes used
T8930 11758-11759 : denotes :
T8931 11760-11765 JJ denotes IL-17
T8932 11766-11771 NN denotes sense
T8933 11771-11772 , denotes ,
T8934 11773-11774 CD denotes 5
T8935 11774-11775 '' denotes '
T8936 11775-11776 : denotes -
T8937 11776-11779 NNP denotes ATG
T8938 11780-11783 NNP denotes ACT
T8939 11784-11787 NNP denotes CCT
T8940 11788-11791 NNP denotes GGG
T8941 11792-11795 NNP denotes AAG
T8942 11796-11799 NNP denotes ACC
T8943 11800-11803 NNP denotes TCA
T8944 11804-11809 NN denotes TTG-3
T8945 11809-11810 '' denotes '
T8946 11810-11811 : denotes ;
T8947 11812-11817 NNP denotes IL-17
T8948 11818-11827 NN denotes antisense
T8949 11827-11828 , denotes ,
T8950 11829-11830 CD denotes 5
T8951 11830-11831 '' denotes '
T8952 11831-11832 : denotes -
T8953 11832-11835 NNP denotes TTA
T8954 11836-11839 NNP denotes GGC
T8955 11840-11843 NNP denotes CAC
T8956 11844-11847 NNP denotes ATG
T8957 11848-11851 NNP denotes GTG
T8958 11852-11855 NNP denotes GAC
T8959 11856-11859 NNP denotes AAT
T8960 11860-11865 NN denotes CGG-3
T8961 11865-11866 '' denotes '
T8962 11866-11867 : denotes ;
T8963 11868-11894 JJ denotes glyceraldehyde-3-phosphate
T8964 11895-11908 NN denotes dehydrogenase
T8965 11909-11910 -LRB- denotes (
T8966 11910-11915 NNP denotes GAPDH
T8967 11915-11916 -RRB- denotes )
T8968 11917-11922 NN denotes sense
T8969 11922-11923 , denotes ,
T8970 11924-11925 CD denotes 5
T8971 11925-11926 '' denotes '
T8972 11926-11927 : denotes -
T8973 11927-11930 NNP denotes CGA
T8974 11931-11934 NNP denotes TGC
T8975 11935-11938 NNP denotes TGG
T8976 11939-11942 NNP denotes GCG
T8977 11943-11946 NNP denotes TGA
T8978 11947-11950 NNP denotes GTA
T8979 11951-11954 NN denotes C-3
T8980 11954-11955 '' denotes '
T8981 11955-11956 : denotes ;
T8982 11957-11962 NNP denotes GAPDH
T8983 11963-11972 NN denotes antisense
T8984 11972-11973 , denotes ,
T8985 11974-11975 CD denotes 5
T8986 11975-11976 '' denotes '
T8987 11976-11977 : denotes -
T8988 11977-11980 NNP denotes CGT
T8989 11981-11984 NNP denotes TCA
T8990 11985-11988 NNP denotes GCT
T8991 11989-11992 NNP denotes CAG
T8992 11993-11996 NNP denotes GGA
T8993 11997-12000 NNP denotes TGA
T8994 12001-12005 NN denotes CC-3
T8995 12005-12006 '' denotes '
T8996 12006-12007 . denotes .
T8997 12008-12017 NNS denotes Reactions
T8998 12018-12022 VBD denotes were
T8999 12023-12032 VBN denotes processed
T9000 12033-12035 IN denotes in
T9001 12036-12037 DT denotes a
T9002 12038-12041 NNP denotes DNA
T9003 12042-12049 JJ denotes thermal
T9004 12050-12056 NN denotes cycler
T9005 12057-12058 -LRB- denotes (
T9006 12058-12070 NNP denotes Perkin-Elmer
T9007 12071-12076 NNP denotes Cetus
T9008 12076-12077 , denotes ,
T9009 12078-12085 NNP denotes Norwalk
T9010 12085-12086 , denotes ,
T9011 12087-12089 NNP denotes CT
T9012 12089-12090 , denotes ,
T9013 12091-12094 NNP denotes USA
T9014 12094-12095 -RRB- denotes )
T9015 12096-12103 IN denotes through
T9016 12104-12110 NNS denotes cycles
T9017 12111-12114 IN denotes for
T9018 12115-12117 CD denotes 30
T9019 12118-12119 PRP denotes s
T9020 12120-12122 IN denotes of
T9021 12123-12135 NN denotes denaturation
T9022 12136-12138 IN denotes at
T9023 12139-12141 CD denotes 94
T9024 12141-12142 CD denotes °
T9025 12142-12143 NNP denotes C
T9026 12143-12144 , denotes ,
T9027 12145-12146 CD denotes 1
T9028 12147-12150 NN denotes min
T9029 12151-12153 IN denotes of
T9030 12154-12163 VBG denotes annealing
T9031 12164-12166 IN denotes at
T9032 12167-12169 CD denotes 56
T9033 12169-12170 CD denotes °
T9034 12170-12171 NNP denotes C
T9035 12172-12175 IN denotes for
T9036 12176-12181 NNP denotes GAPDH
T9037 12182-12185 CC denotes and
T9038 12186-12191 NNP denotes IL-17
T9039 12191-12192 , denotes ,
T9040 12193-12201 VBN denotes followed
T9041 12202-12204 IN denotes by
T9042 12205-12206 CD denotes 1
T9043 12207-12210 NN denotes min
T9044 12211-12213 IN denotes of
T9045 12214-12224 NN denotes elongation
T9046 12225-12227 IN denotes at
T9047 12228-12230 CD denotes 72
T9048 12230-12231 CD denotes °
T9049 12231-12233 NNP denotes C.
T9050 12234-12237 NNP denotes PCR
T9051 12238-12244 NNS denotes rounds
T9052 12245-12249 VBD denotes were
T9053 12250-12258 VBN denotes repeated
T9054 12259-12262 IN denotes for
T9055 12263-12265 CD denotes 25
T9056 12266-12272 NNS denotes cycles
T9057 12273-12277 DT denotes each
T9058 12278-12281 IN denotes for
T9059 12282-12286 DT denotes both
T9060 12287-12292 NNP denotes GAPDH
T9061 12293-12296 CC denotes and
T9062 12297-12302 NNP denotes IL-17
T9063 12302-12303 : denotes ;
T9064 12304-12308 DT denotes this
T9065 12309-12312 VBD denotes was
T9066 12313-12323 VBN denotes determined
T9067 12324-12326 IN denotes as
T9068 12327-12334 VBG denotes falling
T9069 12335-12341 IN denotes within
T9070 12342-12345 DT denotes the
T9071 12346-12357 JJ denotes exponential
T9072 12358-12363 NN denotes phase
T9073 12364-12366 IN denotes of
T9074 12367-12380 NN denotes amplification
T9075 12381-12384 IN denotes for
T9076 12385-12389 DT denotes each
T9077 12390-12398 NN denotes molecule
T9078 12398-12399 . denotes .
T9079 12400-12403 DT denotes The
T9080 12404-12409 NN denotes level
T9081 12410-12412 IN denotes of
T9082 12413-12417 NNP denotes mRNA
T9083 12418-12428 NN denotes expression
T9084 12429-12432 VBD denotes was
T9085 12433-12442 VBN denotes presented
T9086 12443-12445 IN denotes as
T9087 12446-12447 DT denotes a
T9088 12448-12453 NN denotes ratio
T9089 12454-12456 IN denotes of
T9090 12457-12462 NNP denotes IL-17
T9091 12463-12466 NNP denotes PCR
T9092 12467-12474 NN denotes product
T9093 12475-12479 IN denotes over
T9094 12480-12485 NNP denotes GAPDH
T9095 12486-12493 NN denotes product
T9096 12493-12494 . denotes .
T9686 12496-12503 JJ denotes Western
T9687 12504-12508 NN denotes blot
T9688 12509-12517 NN denotes analysis
T9689 12518-12520 IN denotes of
T9690 12521-12524 NNP denotes Akt
T9691 12524-12525 , denotes ,
T9692 12526-12540 VBD denotes phosphorylated
T9693 12541-12544 NNP denotes Akt
T9694 12545-12548 CC denotes and
T9695 12549-12554 NNP denotes IκB-α
T9696 12555-12559 NNP denotes PBMC
T9697 12560-12564 VBD denotes were
T9698 12565-12574 VBN denotes incubated
T9699 12575-12579 IN denotes with
T9700 12580-12588 JJ denotes anti-CD3
T9701 12589-12590 -LRB- denotes (
T9702 12590-12592 CD denotes 10
T9703 12593-12595 NN denotes μg
T9704 12595-12596 NN denotes /
T9705 12596-12598 NN denotes ml
T9706 12598-12599 -RRB- denotes )
T9707 12600-12602 IN denotes in
T9708 12603-12606 DT denotes the
T9709 12607-12615 NN denotes presence
T9710 12616-12618 CC denotes or
T9711 12619-12626 NN denotes absence
T9712 12627-12629 IN denotes of
T9713 12630-12638 NNP denotes LY294002
T9714 12639-12640 -LRB- denotes (
T9715 12640-12642 CD denotes 20
T9716 12643-12645 NNP denotes μM
T9717 12645-12646 -RRB- denotes )
T9718 12646-12647 . denotes .
T9719 12648-12653 IN denotes After
T9720 12654-12664 NN denotes incubation
T9721 12665-12668 IN denotes for
T9722 12669-12670 CD denotes 1
T9723 12671-12675 NN denotes hour
T9724 12675-12676 , denotes ,
T9725 12677-12682 JJ denotes whole
T9726 12683-12687 NN denotes cell
T9727 12688-12695 NNS denotes lysates
T9728 12696-12700 VBD denotes were
T9729 12701-12709 VBN denotes prepared
T9730 12710-12714 IN denotes from
T9731 12715-12720 IN denotes about
T9732 12721-12724 CD denotes 107
T9733 12725-12730 NNS denotes cells
T9734 12731-12733 IN denotes by
T9735 12734-12748 NN denotes homogenization
T9736 12749-12751 IN denotes in
T9737 12752-12755 DT denotes the
T9738 12756-12761 NN denotes lysis
T9739 12762-12768 NN denotes buffer
T9740 12768-12769 , denotes ,
T9741 12770-12773 CC denotes and
T9742 12774-12785 VBD denotes centrifuged
T9743 12786-12788 IN denotes at
T9744 12789-12795 CD denotes 14,000
T9745 12796-12802 NN denotes r.p.m.
T9746 12803-12804 -LRB- denotes (
T9747 12804-12810 CD denotes 19,000
T9748 12811-12812 NN denotes g
T9749 12812-12813 -RRB- denotes )
T9750 12814-12817 IN denotes for
T9751 12818-12820 CD denotes 15
T9752 12821-12824 NN denotes min
T9753 12824-12825 . denotes .
T9754 12826-12833 NN denotes Protein
T9755 12834-12848 NNS denotes concentrations
T9756 12849-12851 IN denotes in
T9757 12852-12855 DT denotes the
T9758 12856-12868 NNS denotes supernatants
T9759 12869-12873 VBD denotes were
T9760 12874-12884 VBN denotes determined
T9761 12885-12889 IN denotes with
T9762 12890-12893 DT denotes the
T9763 12894-12902 NNP denotes Bradford
T9764 12903-12909 NN denotes method
T9765 12910-12911 -LRB- denotes (
T9766 12911-12918 NNP denotes Bio-Rad
T9767 12918-12919 , denotes ,
T9768 12920-12928 NNP denotes Hercules
T9769 12928-12929 , denotes ,
T9770 12930-12932 NNP denotes CA
T9771 12932-12933 , denotes ,
T9772 12934-12937 NNP denotes USA
T9773 12937-12938 -RRB- denotes )
T9774 12938-12939 . denotes .
T9775 12940-12947 NN denotes Protein
T9776 12948-12955 NNS denotes samples
T9777 12956-12960 VBD denotes were
T9778 12961-12970 VBN denotes separated
T9779 12971-12973 IN denotes by
T9780 12974-12976 CD denotes 10
T9781 12976-12977 NN denotes %
T9782 12978-12981 NNP denotes SDS
T9783 12982-12986 NNP denotes PAGE
T9784 12987-12990 CC denotes and
T9785 12991-13002 VBN denotes transferred
T9786 13003-13005 TO denotes to
T9787 13006-13007 DT denotes a
T9788 13008-13022 JJ denotes nitrocellulose
T9789 13023-13031 NN denotes membrane
T9790 13032-13033 -LRB- denotes (
T9791 13033-13041 NNP denotes Amersham
T9792 13042-13051 NNP denotes Pharmacia
T9793 13052-13059 NNP denotes Biotech
T9794 13059-13060 , denotes ,
T9795 13061-13068 NNP denotes Uppsala
T9796 13068-13069 , denotes ,
T9797 13070-13076 NNP denotes Sweden
T9798 13076-13077 -RRB- denotes )
T9799 13077-13078 . denotes .
T9800 13079-13082 IN denotes For
T9801 13083-13090 JJ denotes western
T9802 13091-13104 NN denotes hybridization
T9803 13104-13105 , denotes ,
T9804 13106-13114 NN denotes membrane
T9805 13115-13118 VBD denotes was
T9806 13119-13131 VBN denotes preincubated
T9807 13132-13136 IN denotes with
T9808 13137-13140 CD denotes 0.1
T9809 13140-13141 NN denotes %
T9810 13142-13149 VBD denotes skimmed
T9811 13150-13154 NN denotes milk
T9812 13155-13157 IN denotes in
T9813 13158-13163 NNP denotes TBS-T
T9814 13164-13170 NN denotes buffer
T9815 13171-13172 -LRB- denotes (
T9816 13172-13175 CD denotes 0.1
T9817 13175-13176 NN denotes %
T9818 13177-13182 CD denotes Tween
T9819 13183-13185 CD denotes 20
T9820 13186-13188 IN denotes in
T9821 13189-13202 JJ denotes Tris-buffered
T9822 13203-13209 NN denotes saline
T9823 13209-13210 -RRB- denotes )
T9824 13211-13213 IN denotes at
T9825 13214-13218 NN denotes room
T9826 13219-13230 NN denotes temperature
T9827 13231-13234 IN denotes for
T9828 13235-13236 CD denotes 2
T9829 13237-13242 NNS denotes hours
T9830 13242-13243 , denotes ,
T9831 13244-13248 RB denotes then
T9832 13249-13256 JJ denotes primary
T9833 13257-13267 NNS denotes antibodies
T9834 13268-13275 IN denotes against
T9835 13276-13279 NNP denotes Akt
T9836 13279-13280 , denotes ,
T9837 13281-13295 VBD denotes phosphorylated
T9838 13296-13299 NNP denotes Akt
T9839 13300-13303 CC denotes and
T9840 13304-13309 NNP denotes IκB-α
T9841 13310-13311 -LRB- denotes (
T9842 13311-13315 NNP denotes Cell
T9843 13316-13325 NNP denotes Signaling
T9844 13326-13336 NNP denotes Technology
T9845 13337-13341 NNP denotes Inc.
T9846 13341-13342 , denotes ,
T9847 13343-13350 NNP denotes Beverly
T9848 13350-13351 , denotes ,
T9849 13352-13354 NNP denotes MA
T9850 13354-13355 , denotes ,
T9851 13356-13359 NNP denotes USA
T9852 13359-13360 -RRB- denotes )
T9853 13360-13361 , denotes ,
T9854 13362-13369 VBN denotes diluted
T9855 13370-13376 CD denotes 1:1000
T9856 13377-13379 IN denotes in
T9857 13380-13381 CD denotes 5
T9858 13381-13382 NN denotes %
T9859 13383-13392 NN denotes BSA/TBS-T
T9860 13392-13393 , denotes ,
T9861 13394-13398 VBD denotes were
T9862 13399-13404 VBN denotes added
T9863 13405-13408 CC denotes and
T9864 13409-13418 VBN denotes incubated
T9865 13419-13428 JJ denotes overnight
T9866 13429-13431 IN denotes at
T9867 13432-13433 CD denotes 4
T9868 13433-13434 CD denotes °
T9869 13434-13435 NNP denotes C
T9870 13435-13436 . denotes .
T9871 13437-13442 IN denotes After
T9872 13443-13450 VBG denotes washing
T9873 13451-13455 CD denotes four
T9874 13456-13461 NNS denotes times
T9875 13462-13466 IN denotes with
T9876 13467-13472 NNP denotes TBS-T
T9877 13472-13473 , denotes ,
T9878 13474-13485 JJ denotes horseradish
T9879 13486-13507 JJ denotes peroxidase-conjugated
T9880 13508-13517 JJ denotes secondary
T9881 13518-13528 NNS denotes antibodies
T9882 13529-13533 VBD denotes were
T9883 13534-13539 VBN denotes added
T9884 13540-13543 CC denotes and
T9885 13544-13551 VBN denotes allowed
T9886 13552-13554 TO denotes to
T9887 13555-13563 VB denotes incubate
T9888 13564-13567 IN denotes for
T9889 13568-13569 CD denotes 1
T9890 13570-13574 NN denotes hour
T9891 13575-13577 IN denotes at
T9892 13578-13582 NN denotes room
T9893 13583-13594 NN denotes temperature
T9894 13594-13595 . denotes .
T9895 13596-13601 IN denotes After
T9896 13602-13607 NNP denotes TBS-T
T9897 13608-13615 VBG denotes washing
T9898 13615-13616 , denotes ,
T9899 13617-13627 VBN denotes hybridized
T9900 13628-13633 NNS denotes bands
T9901 13634-13638 VBD denotes were
T9902 13639-13647 VBN denotes detected
T9903 13648-13652 IN denotes with
T9904 13653-13656 DT denotes the
T9905 13657-13665 JJ denotes enhanced
T9906 13666-13683 NN denotes chemiluminescence
T9907 13684-13685 -LRB- denotes (
T9908 13685-13688 NNP denotes ECL
T9909 13688-13689 -RRB- denotes )
T9910 13690-13699 NN denotes detection
T9911 13700-13703 NN denotes kit
T9912 13704-13707 CC denotes and
T9913 13708-13721 JJ denotes Hyperfilm-ECL
T9914 13722-13730 NNS denotes reagents
T9915 13731-13732 -LRB- denotes (
T9916 13732-13740 NNP denotes Amersham
T9917 13741-13750 NNP denotes Pharmacia
T9918 13750-13751 -RRB- denotes )
T9919 13751-13752 . denotes .
T10457 13754-13757 NNP denotes Gel
T10458 13758-13772 JJ denotes mobility-shift
T10459 13773-13778 NN denotes assay
T10460 13779-13781 IN denotes of
T10461 13782-13787 JJ denotes NF-κB
T10462 13788-13795 JJ denotes binding
T10463 13796-13800 NN denotes site
T10464 13801-13808 NNP denotes Nuclear
T10465 13809-13817 NNS denotes proteins
T10466 13818-13822 VBD denotes were
T10467 13823-13832 VBN denotes extracted
T10468 13833-13837 IN denotes from
T10469 13838-13843 IN denotes about
T10470 13844-13845 CD denotes 5
T10471 13846-13847 CD denotes ×
T10472 13848-13851 CD denotes 106
T10473 13852-13856 NNP denotes PBMC
T10474 13856-13857 . denotes .
T10475 13858-13873 NNP denotes Oligonucleotide
T10476 13874-13880 NNS denotes probes
T10477 13881-13893 VBG denotes encompassing
T10478 13894-13897 DT denotes the
T10479 13898-13903 JJ denotes NF-κB
T10480 13904-13911 JJ denotes binding
T10481 13912-13916 NN denotes site
T10482 13917-13919 IN denotes of
T10483 13920-13923 DT denotes the
T10484 13924-13929 JJ denotes human
T10485 13930-13935 NNP denotes IL-17
T10486 13936-13944 NN denotes promoter
T10487 13945-13946 -LRB- denotes (
T10488 13946-13947 CD denotes 5
T10489 13947-13948 '' denotes '
T10490 13948-13949 : denotes -
T10491 13949-13952 NNP denotes ATG
T10492 13953-13956 NNP denotes ACC
T10493 13957-13960 NNP denotes TGG
T10494 13961-13964 NNP denotes AAA
T10495 13965-13968 NNP denotes TAC
T10496 13969-13972 NNP denotes CCA
T10497 13973-13976 NNP denotes AAA
T10498 13977-13982 NN denotes TTC-3
T10499 13982-13983 '' denotes '
T10500 13983-13984 -RRB- denotes )
T10501 13985-13989 VBD denotes were
T10502 13990-13999 VBN denotes generated
T10503 14000-14002 IN denotes by
T10504 14003-14004 CD denotes 5
T10505 14004-14005 '' denotes '
T10506 14005-14006 : denotes -
T10507 14006-14009 NN denotes end
T10508 14010-14018 VBG denotes labeling
T10509 14019-14021 IN denotes of
T10510 14022-14025 DT denotes the
T10511 14026-14031 NN denotes sense
T10512 14032-14038 NN denotes strand
T10513 14039-14043 IN denotes with
T10514 14044-14045 NNP denotes [
T10515 14045-14050 NNP denotes γ-32P
T10516 14050-14051 NNP denotes ]
T10517 14051-14055 NNP denotes dATP
T10518 14056-14057 -LRB- denotes (
T10519 14057-14065 NNP denotes Amersham
T10520 14066-14075 NNP denotes Pharmacia
T10521 14075-14076 -RRB- denotes )
T10522 14077-14080 CC denotes and
T10523 14081-14083 CD denotes T4
T10524 14084-14098 NN denotes polynucleotide
T10525 14099-14105 NN denotes kinase
T10526 14106-14107 -LRB- denotes (
T10527 14107-14113 NNP denotes TaKaRa
T10528 14113-14114 -RRB- denotes )
T10529 14114-14115 . denotes .
T10530 14116-14130 NNP denotes Unincorporated
T10531 14131-14142 NNS denotes nucleotides
T10532 14143-14147 VBD denotes were
T10533 14148-14155 VBN denotes removed
T10534 14156-14158 IN denotes by
T10535 14159-14166 NNP denotes NucTrap
T10536 14167-14172 NN denotes probe
T10537 14173-14185 NN denotes purification
T10538 14186-14193 NNS denotes columns
T10539 14194-14195 -LRB- denotes (
T10540 14195-14205 NNP denotes Stratagene
T10541 14205-14206 , denotes ,
T10542 14207-14209 NNP denotes La
T10543 14210-14215 NNP denotes Jolla
T10544 14215-14216 , denotes ,
T10545 14217-14219 NNP denotes CA
T10546 14219-14220 , denotes ,
T10547 14221-14224 NNP denotes USA
T10548 14224-14225 -RRB- denotes )
T10549 14225-14226 . denotes .
T10550 14227-14234 NNP denotes Nuclear
T10551 14235-14243 NNS denotes extracts
T10552 14244-14245 -LRB- denotes (
T10553 14245-14246 CD denotes 2
T10554 14247-14249 NN denotes μg
T10555 14250-14252 IN denotes of
T10556 14253-14260 NN denotes protein
T10557 14260-14261 -RRB- denotes )
T10558 14262-14266 VBD denotes were
T10559 14267-14276 VBN denotes incubated
T10560 14277-14281 IN denotes with
T10561 14282-14294 JJ denotes radiolabeled
T10562 14295-14298 NNP denotes DNA
T10563 14299-14305 NNS denotes probes
T10564 14306-14307 -LRB- denotes (
T10565 14307-14309 CD denotes 10
T10566 14310-14312 NN denotes ng
T10567 14312-14313 : denotes ;
T10568 14314-14321 CD denotes 100,000
T10569 14322-14328 NN denotes c.p.m.
T10570 14328-14329 -RRB- denotes )
T10571 14330-14333 IN denotes for
T10572 14334-14336 CD denotes 30
T10573 14337-14340 NN denotes min
T10574 14341-14343 IN denotes at
T10575 14344-14348 NN denotes room
T10576 14349-14360 NN denotes temperature
T10577 14361-14363 IN denotes in
T10578 14364-14366 CD denotes 20
T10579 14367-14369 NN denotes μl
T10580 14370-14372 IN denotes of
T10581 14373-14380 JJ denotes binding
T10582 14381-14387 NN denotes buffer
T10583 14388-14398 VBG denotes consisting
T10584 14399-14401 IN denotes of
T10585 14402-14404 CD denotes 20
T10586 14405-14407 NNP denotes mM
T10587 14408-14416 NNP denotes Tris-HCl
T10588 14416-14417 , denotes ,
T10589 14418-14420 NNP denotes pH
T10590 14421-14424 CD denotes 7.9
T10591 14424-14425 , denotes ,
T10592 14426-14428 CD denotes 50
T10593 14429-14431 NNP denotes mM
T10594 14432-14435 NNP denotes KCl
T10595 14435-14436 , denotes ,
T10596 14437-14438 CD denotes 1
T10597 14439-14441 NNP denotes mM
T10598 14442-14456 NN denotes dithiothreitol
T10599 14456-14457 , denotes ,
T10600 14458-14461 CD denotes 0.5
T10601 14462-14464 NNP denotes mM
T10602 14465-14469 NNP denotes EDTA
T10603 14469-14470 , denotes ,
T10604 14471-14472 CD denotes 5
T10605 14472-14473 NN denotes %
T10606 14474-14482 NN denotes glycerol
T10607 14482-14483 , denotes ,
T10608 14484-14485 CD denotes 1
T10609 14486-14491 NN denotes mg/ml
T10610 14492-14495 NNP denotes BSA
T10611 14495-14496 , denotes ,
T10612 14497-14500 CD denotes 0.2
T10613 14500-14501 NN denotes %
T10614 14502-14509 NNP denotes Nonidet
T10615 14510-14513 NNP denotes P40
T10616 14514-14517 CC denotes and
T10617 14518-14520 CD denotes 50
T10618 14521-14523 NN denotes ng
T10619 14523-14524 NN denotes /
T10620 14524-14526 NN denotes μl
T10621 14527-14531 NN denotes poly
T10622 14531-14532 -LRB- denotes (
T10623 14532-14537 JJ denotes dI-dC
T10624 14537-14538 -RRB- denotes )
T10625 14538-14539 . denotes .
T10626 14540-14547 NNS denotes Samples
T10627 14548-14552 VBD denotes were
T10628 14553-14562 VBN denotes subjected
T10629 14563-14565 TO denotes to
T10630 14566-14581 NNS denotes electrophoresis
T10631 14582-14584 IN denotes on
T10632 14585-14598 VBG denotes nondenaturing
T10633 14599-14600 CD denotes 5
T10634 14600-14601 NN denotes %
T10635 14602-14616 NN denotes polyacrylamide
T10636 14617-14621 NNS denotes gels
T10637 14622-14624 IN denotes in
T10638 14625-14628 CD denotes 0.5
T10639 14629-14630 CD denotes ×
T10640 14631-14647 NNP denotes Tris-borate-EDTA
T10641 14648-14654 NN denotes buffer
T10642 14655-14656 -LRB- denotes (
T10643 14656-14658 NNP denotes pH
T10644 14659-14662 CD denotes 8.0
T10645 14662-14663 -RRB- denotes )
T10646 14664-14666 IN denotes at
T10647 14667-14670 CD denotes 100
T10648 14671-14673 NNP denotes V.
T10649 14674-14678 NNP denotes Gels
T10650 14679-14683 VBD denotes were
T10651 14684-14689 VBN denotes dried
T10652 14690-14695 IN denotes under
T10653 14696-14702 NN denotes vacuum
T10654 14703-14706 CC denotes and
T10655 14707-14714 VBN denotes exposed
T10656 14715-14717 TO denotes to
T10657 14718-14723 NNP denotes Kodak
T10658 14724-14730 NNP denotes X-OMAT
T10659 14731-14735 NN denotes film
T10660 14736-14738 IN denotes at
T10661 14739-14742 CD denotes -70
T10662 14742-14743 CD denotes °
T10663 14743-14744 NNP denotes C
T10664 14745-14749 IN denotes with
T10665 14750-14762 VBG denotes intensifying
T10666 14763-14770 NNS denotes screens
T10667 14770-14771 . denotes .
T10668 14772-14778 NN denotes Rabbit
T10669 14779-14789 NN denotes polyclonal
T10670 14790-14800 NNS denotes antibodies
T10671 14801-14808 IN denotes against
T10672 14809-14814 NNP denotes NF-κB
T10673 14815-14823 VBZ denotes subunits
T10674 14824-14827 CD denotes p50
T10675 14827-14828 , denotes ,
T10676 14829-14832 CD denotes p65
T10677 14833-14836 CC denotes and
T10678 14837-14842 NNP denotes c-Rel
T10679 14843-14847 VBD denotes were
T10680 14848-14852 IN denotes from
T10681 14853-14858 NNP denotes Santa
T10682 14859-14863 NNP denotes Cruz
T10683 14864-14877 NNP denotes Biotechnology
T10684 14878-14879 -LRB- denotes (
T10685 14879-14884 NNP denotes Santa
T10686 14885-14889 NNP denotes Cruz
T10687 14889-14890 , denotes ,
T10688 14891-14893 NNP denotes CA
T10689 14893-14894 , denotes ,
T10690 14895-14898 NNP denotes USA
T10691 14898-14899 -RRB- denotes )
T10692 14899-14900 . denotes .
T10901 14902-14906 NNP denotes Cell
T10902 14907-14916 NN denotes viability
T10903 14917-14918 -LRB- denotes (
T10904 14918-14924 NNP denotes Trypan
T10905 14925-14929 JJ denotes blue
T10906 14930-14933 NN denotes dye
T10907 14934-14943 NN denotes exclusion
T10908 14944-14949 NN denotes assay
T10909 14949-14950 -RRB- denotes )
T10910 14951-14954 IN denotes For
T10911 14955-14959 NN denotes cell
T10912 14960-14969 NN denotes viability
T10913 14970-14976 NNS denotes assays
T10914 14976-14977 , denotes ,
T10915 14978-14981 DT denotes the
T10916 14982-14988 NN denotes trypan
T10917 14989-14993 JJ denotes blue
T10918 14994-14997 NN denotes dye
T10919 14998-15007 NN denotes exclusion
T10920 15008-15014 NN denotes method
T10921 15015-15018 VBD denotes was
T10922 15019-15023 VBN denotes used
T10923 15024-15026 TO denotes to
T10924 15027-15035 VB denotes evaluate
T10925 15036-15039 DT denotes the
T10926 15040-15049 NN denotes potential
T10927 15050-15052 IN denotes of
T10928 15053-15059 JJ denotes direct
T10929 15060-15069 JJ denotes cytotoxic
T10930 15070-15076 NN denotes effect
T10931 15077-15079 IN denotes of
T10932 15080-15090 NNS denotes inhibitors
T10933 15091-15093 IN denotes on
T10934 15094-15099 NNS denotes cells
T10935 15099-15100 . denotes .
T10936 15101-15106 IN denotes After
T10937 15107-15117 NN denotes incubation
T10938 15118-15121 IN denotes for
T10939 15122-15124 CD denotes 24
T10940 15125-15130 NNS denotes hours
T10941 15130-15131 , denotes ,
T10942 15132-15135 DT denotes the
T10943 15136-15141 NNS denotes cells
T10944 15142-15146 VBD denotes were
T10945 15147-15156 VBN denotes harvested
T10946 15157-15160 CC denotes and
T10947 15161-15164 DT denotes the
T10948 15165-15175 NN denotes percentage
T10949 15176-15180 NN denotes cell
T10950 15181-15190 NN denotes viability
T10951 15191-15194 VBD denotes was
T10952 15195-15205 VBN denotes calculated
T10953 15206-15210 IN denotes with
T10954 15211-15214 DT denotes the
T10955 15215-15222 NN denotes formula
T10956 15223-15226 CD denotes 100
T10957 15227-15228 NN denotes ×
T10958 15229-15230 -LRB- denotes (
T10959 15230-15236 NN denotes number
T10960 15237-15239 IN denotes of
T10961 15240-15246 JJ denotes viable
T10962 15247-15259 NN denotes cells/number
T10963 15260-15262 IN denotes of
T10964 15263-15267 DT denotes both
T10965 15268-15274 JJ denotes viable
T10966 15275-15278 CC denotes and
T10967 15279-15283 JJ denotes dead
T10968 15284-15289 NNS denotes cells
T10969 15289-15290 -RRB- denotes )
T10970 15291-15292 NNP denotes [
T10971 15292-15294 CD denotes 21
T10972 15294-15295 NNP denotes ]
T10973 15295-15296 . denotes .
T11046 15298-15309 NNP denotes Statistical
T11047 15310-15318 NN denotes analysis
T11048 15319-15323 NNS denotes Data
T11049 15324-15327 VBP denotes are
T11050 15328-15337 VBN denotes expressed
T11051 15338-15340 IN denotes as
T11052 15341-15346 NNS denotes means
T11053 15347-15348 VBP denotes ±
T11054 15349-15352 NNP denotes SEM
T11055 15352-15353 . denotes .
T11056 15354-15365 NNP denotes Statistical
T11057 15366-15374 NN denotes analysis
T11058 15375-15378 VBD denotes was
T11059 15379-15388 VBN denotes performed
T11060 15389-15393 IN denotes with
T11061 15394-15401 NN denotes Student
T11062 15401-15403 POS denotes 's
T11063 15404-15410 JJS denotes t-test
T11064 15411-15414 IN denotes for
T11065 15415-15422 VBN denotes matched
T11066 15423-15428 NNS denotes pairs
T11067 15428-15429 . denotes .
T11068 15430-15431 NNP denotes P
T11069 15432-15438 VBZ denotes values
T11070 15439-15443 JJR denotes less
T11071 15444-15448 IN denotes than
T11072 15449-15453 CD denotes 0.05
T11073 15454-15458 VBD denotes were
T11074 15459-15469 VBN denotes considered
T11075 15470-15481 JJ denotes significant
T11076 15481-15482 . denotes .
T11434 15493-15498 NN denotes IL-17
T11435 15499-15509 NN denotes production
T11436 15510-15512 IN denotes in
T11437 15513-15517 NNP denotes PBMC
T11438 15518-15522 IN denotes from
T11439 15523-15531 NNS denotes patients
T11440 15532-15536 IN denotes with
T11441 15537-15539 NNP denotes RA
T11442 15539-15540 , denotes ,
T11443 15541-15549 NNS denotes patients
T11444 15550-15554 IN denotes with
T11445 15555-15557 NNP denotes OA
T11446 15558-15561 CC denotes and
T11447 15562-15568 JJ denotes normal
T11448 15569-15580 NNS denotes individuals
T11449 15581-15585 NNP denotes PBMC
T11450 15586-15590 VBD denotes were
T11451 15591-15600 VBN denotes separated
T11452 15601-15604 CC denotes and
T11453 15605-15613 VBN denotes cultured
T11454 15614-15618 IN denotes with
T11455 15619-15622 NNP denotes PHA
T11456 15623-15624 -LRB- denotes (
T11457 15624-15625 CD denotes 5
T11458 15626-15628 NN denotes μg
T11459 15628-15629 NN denotes /
T11460 15629-15631 NN denotes ml
T11461 15631-15632 -RRB- denotes )
T11462 15633-15637 IN denotes from
T11463 15638-15646 NNS denotes patients
T11464 15647-15651 IN denotes with
T11465 15652-15654 NNP denotes RA
T11466 15654-15655 , denotes ,
T11467 15656-15664 NNS denotes patients
T11468 15665-15669 IN denotes with
T11469 15670-15672 NNP denotes OA
T11470 15672-15673 , denotes ,
T11471 15674-15677 CC denotes and
T11472 15678-15689 JJ denotes age-matched
T11473 15690-15696 JJ denotes normal
T11474 15697-15705 NNS denotes controls
T11475 15705-15706 : denotes ;
T11476 15707-15712 NNP denotes IL-17
T11477 15713-15719 NNS denotes levels
T11478 15720-15724 VBD denotes were
T11479 15725-15729 RB denotes then
T11480 15730-15740 VBN denotes determined
T11481 15741-15743 IN denotes in
T11482 15744-15747 DT denotes the
T11483 15748-15755 NN denotes culture
T11484 15756-15768 NNS denotes supernatants
T11485 15769-15770 -LRB- denotes (
T11486 15770-15774 NNP denotes Fig.
T11487 15775-15776 CD denotes 1
T11488 15776-15777 -RRB- denotes )
T11489 15777-15778 . denotes .
T11490 15779-15787 IN denotes Although
T11491 15788-15791 DT denotes the
T11492 15792-15799 NNS denotes amounts
T11493 15800-15802 IN denotes of
T11494 15803-15808 JJ denotes basal
T11495 15809-15814 NNP denotes IL-17
T11496 15815-15824 NN denotes secretion
T11497 15825-15829 VBD denotes were
T11498 15830-15833 RB denotes not
T11499 15834-15843 JJ denotes different
T11500 15844-15851 IN denotes between
T11501 15852-15854 NNP denotes RA
T11502 15854-15855 , denotes ,
T11503 15856-15858 NNP denotes OA
T11504 15859-15862 CC denotes and
T11505 15863-15869 JJ denotes normal
T11506 15870-15878 NNS denotes controls
T11507 15879-15880 -LRB- denotes (
T11508 15880-15882 CD denotes 62
T11509 15883-15884 NN denotes ±
T11510 15885-15887 CD denotes 31
T11511 15887-15888 , denotes ,
T11512 15889-15891 CD denotes 43
T11513 15892-15893 NN denotes ±
T11514 15894-15896 CD denotes 19
T11515 15897-15900 CC denotes and
T11516 15901-15903 CD denotes 43
T11517 15904-15905 NN denotes ±
T11518 15906-15908 CD denotes 10
T11519 15909-15914 NN denotes pg/ml
T11520 15914-15915 , denotes ,
T11521 15916-15928 RB denotes respectively
T11522 15928-15929 -RRB- denotes )
T11523 15929-15930 , denotes ,
T11524 15931-15934 DT denotes the
T11525 15935-15940 NNP denotes IL-17
T11526 15941-15951 NN denotes production
T11527 15952-15962 VBN denotes stimulated
T11528 15963-15965 IN denotes by
T11529 15966-15969 NNP denotes PHA
T11530 15970-15973 VBD denotes was
T11531 15974-15987 RB denotes significantly
T11532 15988-15994 JJR denotes higher
T11533 15995-15997 IN denotes in
T11534 15998-16000 NNP denotes RA
T11535 16001-16005 NNP denotes PBMC
T11536 16006-16010 IN denotes than
T11537 16011-16013 IN denotes in
T11538 16014-16019 DT denotes those
T11539 16020-16024 IN denotes from
T11540 16025-16027 NNP denotes OA
T11541 16028-16031 CC denotes and
T11542 16032-16040 NNS denotes controls
T11543 16041-16042 -LRB- denotes (
T11544 16042-16045 CD denotes 768
T11545 16046-16047 CD denotes ±
T11546 16048-16051 CD denotes 295
T11547 16052-16058 IN denotes versus
T11548 16059-16062 CD denotes 463
T11549 16063-16064 CD denotes ±
T11550 16065-16068 CD denotes 211
T11551 16069-16074 VBN denotes pg/ml
T11552 16075-16076 NNP denotes [
T11553 16076-16077 NNP denotes P
T11554 16078-16079 VBD denotes <
T11555 16080-16084 CD denotes 0.05
T11556 16084-16085 NNP denotes ]
T11557 16086-16089 CC denotes and
T11558 16090-16093 CD denotes 241
T11559 16094-16095 CD denotes ±
T11560 16096-16098 CD denotes 29
T11561 16099-16104 NN denotes pg/ml
T11562 16105-16106 NNP denotes [
T11563 16106-16107 NNP denotes P
T11564 16108-16109 VBD denotes <
T11565 16110-16115 CD denotes 0.001
T11566 16115-16116 NNP denotes ]
T11567 16116-16117 -RRB- denotes )
T11568 16117-16118 . denotes .
T12114 16120-16129 VBN denotes Increased
T12115 16130-16135 JJ denotes IL-17
T12116 16136-16146 NN denotes production
T12117 16147-16149 IN denotes in
T12118 16150-16154 NNP denotes PBMC
T12119 16155-16157 IN denotes of
T12120 16158-16166 NNS denotes patients
T12121 16167-16171 IN denotes with
T12122 16172-16174 NNP denotes RA
T12123 16175-16177 IN denotes by
T12124 16178-16186 JJ denotes anti-CD3
T12125 16187-16193 CC denotes and/or
T12126 16194-16203 JJ denotes anti-CD28
T12127 16203-16204 , denotes ,
T12128 16205-16208 CC denotes and
T12129 16209-16212 NNP denotes PHA
T12130 16213-16220 IN denotes Because
T12131 16221-16226 NNP denotes IL-17
T12132 16227-16230 VBD denotes was
T12133 16231-16238 RB denotes already
T12134 16239-16244 VBN denotes known
T12135 16245-16249 IN denotes from
T12136 16250-16257 JJR denotes earlier
T12137 16258-16265 NNS denotes reports
T12138 16266-16268 TO denotes to
T12139 16269-16271 VB denotes be
T12140 16272-16280 VBN denotes produced
T12141 16281-16287 RB denotes mainly
T12142 16288-16290 IN denotes by
T12143 16291-16300 VBN denotes activated
T12144 16301-16302 NNP denotes T
T12145 16303-16308 NNS denotes cells
T12146 16308-16309 , denotes ,
T12147 16310-16312 PRP denotes we
T12148 16313-16325 VBD denotes investigated
T12149 16326-16329 DT denotes the
T12150 16330-16336 NN denotes effect
T12151 16337-16339 IN denotes of
T12152 16340-16349 JJ denotes different
T12153 16350-16364 NNS denotes concentrations
T12154 16365-16367 IN denotes of
T12155 16368-16376 JJ denotes anti-CD3
T12156 16377-16378 -LRB- denotes (
T12157 16378-16379 CD denotes 1
T12158 16379-16380 , denotes ,
T12159 16381-16382 CD denotes 5
T12160 16383-16386 CC denotes and
T12161 16387-16389 CD denotes 10
T12162 16390-16392 NN denotes μg
T12163 16392-16393 NN denotes /
T12164 16393-16395 NN denotes ml
T12165 16395-16396 -RRB- denotes )
T12166 16397-16399 IN denotes as
T12167 16400-16401 DT denotes a
T12168 16402-16403 NNP denotes T
T12169 16404-16408 NN denotes cell
T12170 16409-16419 NN denotes activation
T12171 16419-16420 , denotes ,
T12172 16421-16426 WDT denotes which
T12173 16427-16433 VBD denotes showed
T12174 16434-16435 DT denotes a
T12175 16436-16450 JJ denotes dose-dependent
T12176 16451-16459 NN denotes increase
T12177 16460-16462 IN denotes in
T12178 16463-16468 NNP denotes IL-17
T12179 16469-16475 NNS denotes levels
T12180 16476-16477 -LRB- denotes (
T12181 16477-16481 NNS denotes data
T12182 16482-16485 RB denotes not
T12183 16486-16491 VBN denotes shown
T12184 16491-16492 -RRB- denotes )
T12185 16492-16493 . denotes .
T12186 16494-16496 IN denotes On
T12187 16497-16500 DT denotes the
T12188 16501-16506 NN denotes basis
T12189 16507-16509 IN denotes of
T12190 16510-16514 DT denotes this
T12191 16514-16515 , denotes ,
T12192 16516-16518 PRP denotes we
T12193 16519-16524 VBD denotes chose
T12194 16525-16527 CD denotes 10
T12195 16528-16530 NN denotes μg
T12196 16530-16531 NN denotes /
T12197 16531-16533 NN denotes ml
T12198 16534-16536 IN denotes as
T12199 16537-16538 DT denotes a
T12200 16539-16550 NN denotes stimulation
T12201 16551-16564 NN denotes concentration
T12202 16565-16568 IN denotes for
T12203 16569-16577 JJ denotes anti-CD3
T12204 16577-16578 . denotes .
T12205 16579-16581 IN denotes As
T12206 16582-16587 VBN denotes shown
T12207 16588-16590 IN denotes in
T12208 16591-16596 NNP denotes Table
T12209 16597-16598 CD denotes 1
T12210 16598-16599 , denotes ,
T12211 16600-16608 JJ denotes anti-CD3
T12212 16609-16622 RB denotes significantly
T12213 16623-16634 JJ denotes upregulated
T12214 16635-16640 NN denotes IL-17
T12215 16641-16651 NN denotes production
T12216 16652-16654 IN denotes up
T12217 16655-16657 TO denotes to
T12218 16658-16666 CD denotes 3.7-fold
T12219 16666-16667 , denotes ,
T12220 16668-16671 CC denotes and
T12221 16672-16675 DT denotes the
T12222 16676-16687 NN denotes combination
T12223 16688-16690 IN denotes of
T12224 16691-16700 JJ denotes anti-CD28
T12225 16701-16704 CC denotes and
T12226 16705-16713 JJ denotes anti-CD3
T12227 16714-16722 VBN denotes produced
T12228 16723-16727 RBR denotes more
T12229 16728-16733 JJ denotes IL-17
T12230 16734-16735 -LRB- denotes (
T12231 16735-16748 RB denotes approximately
T12232 16749-16754 CD denotes 1.3-1
T12233 16754-16756 SYM denotes .5
T12234 16756-16757 : denotes -
T12235 16757-16761 VB denotes fold
T12236 16761-16762 -RRB- denotes )
T12237 16763-16767 IN denotes than
T12238 16768-16776 JJ denotes anti-CD3
T12239 16777-16782 RB denotes alone
T12240 16782-16783 . denotes .
T12241 16784-16795 RB denotes Furthermore
T12242 16795-16796 , denotes ,
T12243 16797-16801 WRB denotes when
T12244 16802-16811 VBN denotes incubated
T12245 16812-16816 IN denotes with
T12246 16817-16818 NNP denotes T
T12247 16819-16823 NN denotes cell
T12248 16824-16832 NNS denotes mitogens
T12249 16833-16837 JJ denotes such
T12250 16838-16840 IN denotes as
T12251 16841-16844 NNP denotes PHA
T12252 16844-16845 , denotes ,
T12253 16846-16855 VBD denotes increased
T12254 16856-16861 NNP denotes IL-17
T12255 16862-16872 NN denotes production
T12256 16873-16876 VBD denotes was
T12257 16877-16881 RBR denotes more
T12258 16882-16892 JJ denotes pronounced
T12259 16893-16897 IN denotes than
T12260 16898-16902 IN denotes with
T12261 16903-16911 JJ denotes anti-CD3
T12262 16912-16915 CC denotes and
T12263 16916-16925 JJ denotes anti-CD28
T12264 16926-16927 -LRB- denotes (
T12265 16927-16930 CD denotes 588
T12266 16931-16932 CD denotes ±
T12267 16933-16935 CD denotes 85
T12268 16936-16942 IN denotes versus
T12269 16943-16946 CD denotes 211
T12270 16947-16948 CD denotes ±
T12271 16949-16950 CD denotes 1
T12272 16951-16956 NN denotes pg/ml
T12273 16956-16957 : denotes ;
T12274 16958-16959 NNP denotes P
T12275 16960-16961 NNP denotes <
T12276 16962-16966 CD denotes 0.05
T12277 16966-16967 -RRB- denotes )
T12278 16967-16968 . denotes .
T12997 16970-16980 NN denotes Regulation
T12998 16981-16983 IN denotes of
T12999 16984-16989 JJ denotes IL-17
T13000 16990-17000 NN denotes production
T13001 17001-17003 IN denotes in
T13002 17004-17006 NNP denotes RA
T13003 17007-17011 NNP denotes PBMC
T13004 17012-17014 IN denotes by
T13005 17015-17027 JJ denotes inflammatory
T13006 17028-17037 NNS denotes cytokines
T13007 17038-17041 CC denotes and
T13008 17042-17052 NNS denotes chemokines
T13009 17053-17060 IN denotes Because
T13010 17061-17063 NNP denotes RA
T13011 17064-17068 NNP denotes PBMC
T13012 17069-17076 VBP denotes include
T13013 17077-17084 JJ denotes several
T13014 17085-17089 NN denotes cell
T13015 17090-17095 NNS denotes types
T13016 17096-17098 IN denotes in
T13017 17099-17107 NN denotes addition
T13018 17108-17110 TO denotes to
T13019 17111-17112 NNP denotes T
T13020 17113-17118 NNS denotes cells
T13021 17118-17119 , denotes ,
T13022 17120-17124 DT denotes some
T13023 17125-17137 JJ denotes inflammatory
T13024 17138-17147 NNS denotes cytokines
T13025 17148-17156 VBN denotes released
T13026 17157-17161 IN denotes from
T13027 17162-17173 NNS denotes macrophages
T13028 17174-17177 CC denotes and
T13029 17178-17183 JJ denotes other
T13030 17184-17195 NNS denotes lymphocytes
T13031 17196-17201 MD denotes might
T13032 17202-17206 VB denotes have
T13033 17207-17215 VBN denotes affected
T13034 17216-17219 DT denotes the
T13035 17220-17230 NN denotes production
T13036 17231-17233 IN denotes of
T13037 17234-17239 NNP denotes IL-17
T13038 17240-17244 IN denotes from
T13039 17245-17246 NNP denotes T
T13040 17247-17252 NNS denotes cells
T13041 17252-17253 . denotes .
T13042 17254-17256 TO denotes To
T13043 17257-17265 VB denotes evaluate
T13044 17266-17269 DT denotes the
T13045 17270-17277 NNS denotes effects
T13046 17278-17280 IN denotes of
T13047 17281-17293 JJ denotes inflammatory
T13048 17294-17303 NNS denotes cytokines
T13049 17304-17312 VBN denotes released
T13050 17313-17315 IN denotes by
T13051 17316-17325 VBN denotes activated
T13052 17326-17330 NNP denotes PBMC
T13053 17330-17331 , denotes ,
T13054 17332-17334 PRP denotes we
T13055 17335-17341 VBD denotes tested
T13056 17342-17345 DT denotes the
T13057 17346-17353 NNS denotes effects
T13058 17354-17356 IN denotes of
T13059 17357-17364 JJ denotes several
T13060 17365-17374 NNS denotes cytokines
T13061 17375-17378 CC denotes and
T13062 17379-17389 NNS denotes chemokines
T13063 17390-17392 IN denotes on
T13064 17393-17398 NN denotes IL-17
T13065 17399-17409 NN denotes production
T13066 17409-17410 . denotes .
T13067 17411-17413 PRP denotes We
T13068 17414-17422 VBD denotes detected
T13069 17423-17425 DT denotes an
T13070 17426-17434 NN denotes increase
T13071 17435-17437 IN denotes in
T13072 17438-17443 NNP denotes IL-17
T13073 17444-17449 NN denotes level
T13074 17450-17455 IN denotes after
T13075 17456-17467 NN denotes stimulation
T13076 17468-17472 IN denotes with
T13077 17473-17478 NNP denotes IL-15
T13078 17479-17480 -LRB- denotes (
T13079 17480-17482 CD denotes 10
T13080 17483-17488 NN denotes ng/ml
T13081 17488-17489 -RRB- denotes )
T13082 17489-17490 , denotes ,
T13083 17491-17498 IN denotes whereas
T13084 17499-17503 IN denotes with
T13085 17504-17509 NN denotes IL-1β
T13086 17510-17511 -LRB- denotes (
T13087 17511-17513 CD denotes 10
T13088 17514-17519 NN denotes ng/ml
T13089 17519-17520 -RRB- denotes )
T13090 17520-17521 , denotes ,
T13091 17522-17527 NNP denotes TNF-α
T13092 17528-17529 -LRB- denotes (
T13093 17529-17531 CD denotes 10
T13094 17532-17537 NN denotes ng/ml
T13095 17537-17538 -RRB- denotes )
T13096 17538-17539 , denotes ,
T13097 17540-17545 NN denotes IL-18
T13098 17546-17547 -LRB- denotes (
T13099 17547-17549 CD denotes 10
T13100 17550-17555 NN denotes ng/ml
T13101 17555-17556 -RRB- denotes )
T13102 17557-17559 CC denotes or
T13103 17560-17565 NNP denotes TGF-β
T13104 17566-17567 -LRB- denotes (
T13105 17567-17569 CD denotes 10
T13106 17570-17575 NN denotes ng/ml
T13107 17575-17576 -RRB- denotes )
T13108 17577-17580 DT denotes the
T13109 17581-17587 NNS denotes levels
T13110 17588-17590 IN denotes in
T13111 17591-17596 NNP denotes IL-17
T13112 17597-17601 VBD denotes were
T13113 17602-17611 JJ denotes unchanged
T13114 17612-17613 -LRB- denotes (
T13115 17613-17617 NNP denotes Fig.
T13116 17618-17620 CD denotes 2a
T13117 17620-17621 -RRB- denotes )
T13118 17621-17622 . denotes .
T13119 17623-17627 WRB denotes When
T13120 17628-17635 VBN denotes treated
T13121 17636-17640 IN denotes with
T13122 17641-17646 NNP denotes MCP-1
T13123 17647-17648 -LRB- denotes (
T13124 17648-17650 CD denotes 10
T13125 17651-17656 NN denotes ng/ml
T13126 17656-17657 -RRB- denotes )
T13127 17658-17660 CC denotes or
T13128 17661-17665 NNP denotes IL-6
T13129 17666-17667 -LRB- denotes (
T13130 17667-17669 CD denotes 10
T13131 17670-17675 NN denotes ng/ml
T13132 17675-17676 -RRB- denotes )
T13133 17676-17677 , denotes ,
T13134 17678-17689 JJ denotes significant
T13135 17690-17703 NNS denotes upregulations
T13136 17704-17706 IN denotes of
T13137 17707-17712 NNP denotes IL-17
T13138 17713-17721 NNS denotes proteins
T13139 17722-17726 VBD denotes were
T13140 17727-17735 VBN denotes observed
T13141 17736-17737 -LRB- denotes (
T13142 17737-17739 CD denotes 62
T13143 17740-17741 NN denotes ±
T13144 17742-17744 CD denotes 42
T13145 17745-17748 CC denotes and
T13146 17749-17751 CD denotes 50
T13147 17752-17753 NN denotes ±
T13148 17754-17756 CD denotes 10
T13149 17757-17763 CC denotes versus
T13150 17764-17766 CD denotes 31
T13151 17767-17768 NN denotes ±
T13152 17769-17771 CD denotes 11
T13153 17772-17777 NN denotes pg/ml
T13154 17777-17778 , denotes ,
T13155 17779-17791 RB denotes respectively
T13156 17791-17792 : denotes ;
T13157 17793-17794 NNP denotes P
T13158 17795-17796 NNP denotes <
T13159 17797-17801 CD denotes 0.05
T13160 17801-17802 -RRB- denotes )
T13161 17802-17803 , denotes ,
T13162 17804-17811 IN denotes whereas
T13163 17812-17816 NN denotes none
T13164 17817-17820 VBD denotes was
T13165 17821-17829 VBN denotes observed
T13166 17830-17834 IN denotes with
T13167 17835-17839 NN denotes IL-8
T13168 17840-17841 -LRB- denotes (
T13169 17841-17843 CD denotes 10
T13170 17844-17849 NN denotes ng/ml
T13171 17849-17850 -RRB- denotes )
T13172 17850-17851 , denotes ,
T13173 17852-17858 NNP denotes MIP-1α
T13174 17859-17860 -LRB- denotes (
T13175 17860-17862 CD denotes 10
T13176 17863-17868 NN denotes ng/ml
T13177 17868-17869 -RRB- denotes )
T13178 17870-17872 CC denotes or
T13179 17873-17879 NNP denotes MIP-1β
T13180 17880-17881 -LRB- denotes (
T13181 17881-17883 CD denotes 10
T13182 17884-17889 NN denotes ng/ml
T13183 17889-17890 -RRB- denotes )
T13184 17891-17892 -LRB- denotes (
T13185 17892-17896 NNP denotes Fig.
T13186 17897-17899 CD denotes 2b
T13187 17899-17900 -RRB- denotes )
T13188 17900-17901 . denotes .
T14019 17903-17913 NNP denotes Inhibition
T14020 17914-17916 IN denotes of
T14021 17917-17922 NNP denotes IL-17
T14022 17923-17933 NN denotes production
T14023 17934-17936 IN denotes by
T14024 17937-17943 NN denotes signal
T14025 17944-17956 NN denotes transduction
T14026 17957-17967 NNS denotes inhibitors
T14027 17968-17971 CC denotes and
T14028 17972-17986 JJ denotes anti-rheumatic
T14029 17987-17992 NNS denotes drugs
T14030 17993-17999 VBG denotes Having
T14031 18000-18008 VBN denotes observed
T14032 18009-18012 DT denotes the
T14033 18013-18022 VBN denotes increased
T14034 18023-18028 NN denotes IL-17
T14035 18029-18039 NN denotes production
T14036 18040-18042 IN denotes in
T14037 18043-18045 NNP denotes RA
T14038 18046-18050 NNP denotes PBMC
T14039 18050-18051 , denotes ,
T14040 18052-18054 PRP denotes it
T14041 18055-18058 VBD denotes was
T14042 18059-18068 JJ denotes important
T14043 18069-18071 TO denotes to
T14044 18072-18076 VB denotes know
T14045 18077-18082 WDT denotes which
T14046 18083-18089 NN denotes signal
T14047 18090-18102 NN denotes transduction
T14048 18103-18111 NNS denotes pathways
T14049 18112-18116 VBD denotes were
T14050 18117-18125 VBN denotes involved
T14051 18125-18126 . denotes .
T14052 18127-18129 IN denotes As
T14053 18130-18141 VBN denotes illustrated
T14054 18142-18144 IN denotes in
T14055 18145-18149 NNP denotes Fig.
T14056 18150-18151 CD denotes 3
T14057 18151-18152 , denotes ,
T14058 18153-18155 DT denotes an
T14059 18156-18167 JJ denotes significant
T14060 18168-18176 NN denotes decrease
T14061 18177-18179 IN denotes in
T14062 18180-18196 JJ denotes anti-CD3-induced
T14063 18197-18202 NN denotes IL-17
T14064 18203-18213 NN denotes production
T14065 18214-18217 VBD denotes was
T14066 18218-18226 VBN denotes observed
T14067 18227-18231 WRB denotes when
T14068 18232-18244 VBN denotes co-incubated
T14069 18245-18249 IN denotes with
T14070 18250-18255 JJ denotes NF-κB
T14071 18256-18265 NN denotes inhibitor
T14072 18265-18266 , denotes ,
T14073 18267-18271 NNP denotes PDTC
T14074 18272-18275 CC denotes and
T14075 18276-18289 NN denotes dexamethasone
T14076 18290-18292 IN denotes in
T14077 18293-18303 NN denotes comparison
T14078 18304-18308 IN denotes with
T14079 18309-18317 JJ denotes anti-CD3
T14080 18318-18323 RB denotes alone
T14081 18324-18325 -LRB- denotes (
T14082 18325-18327 CD denotes 38
T14083 18328-18329 NN denotes ±
T14084 18330-18331 CD denotes 5
T14085 18332-18335 CC denotes and
T14086 18336-18338 CD denotes 54
T14087 18339-18340 NN denotes ±
T14088 18341-18343 CD denotes 11
T14089 18344-18350 CC denotes versus
T14090 18351-18353 CD denotes 98
T14091 18354-18355 NN denotes ±
T14092 18356-18358 CD denotes 19
T14093 18359-18364 NN denotes pg/ml
T14094 18364-18365 , denotes ,
T14095 18366-18378 RB denotes respectively
T14096 18378-18379 : denotes ;
T14097 18380-18381 NNP denotes P
T14098 18382-18383 NNP denotes <
T14099 18384-18388 CD denotes 0.05
T14100 18388-18389 -RRB- denotes )
T14101 18389-18390 . denotes .
T14102 18391-18399 CD denotes LY294002
T14103 18400-18403 CC denotes and
T14104 18404-18414 NN denotes wortmannin
T14105 18414-18415 , denotes ,
T14106 18416-18418 IN denotes as
T14107 18419-18421 DT denotes an
T14108 18422-18431 NN denotes inhibitor
T14109 18432-18434 IN denotes of
T14110 18435-18439 NNP denotes PI3K
T14111 18439-18440 , denotes ,
T14112 18441-18445 RB denotes also
T14113 18446-18454 RB denotes markedly
T14114 18455-18464 VBD denotes inhibited
T14115 18465-18468 DT denotes the
T14116 18469-18485 JJ denotes anti-CD3-induced
T14117 18486-18491 NN denotes IL-17
T14118 18492-18502 NN denotes production
T14119 18503-18505 IN denotes in
T14120 18506-18508 NNP denotes RA
T14121 18509-18513 NNP denotes PBMC
T14122 18514-18515 -LRB- denotes (
T14123 18515-18517 CD denotes 98
T14124 18518-18519 NN denotes ±
T14125 18520-18522 CD denotes 19
T14126 18523-18529 CC denotes versus
T14127 18530-18532 CD denotes 38
T14128 18533-18534 NN denotes ±
T14129 18535-18537 CD denotes 10
T14130 18538-18543 NN denotes pg/ml
T14131 18544-18545 NNP denotes [
T14132 18545-18546 NNP denotes P
T14133 18547-18548 VBD denotes <
T14134 18549-18554 CD denotes 0.005
T14135 18554-18555 NNP denotes ]
T14136 18556-18559 CC denotes and
T14137 18560-18562 CD denotes 48
T14138 18563-18564 NN denotes ±
T14139 18565-18566 CD denotes 4
T14140 18567-18572 NN denotes pg/ml
T14141 18573-18574 NNP denotes [
T14142 18574-18575 NNP denotes P
T14143 18576-18577 VBD denotes <
T14144 18578-18582 CD denotes 0.05
T14145 18582-18583 NNP denotes ]
T14146 18583-18584 , denotes ,
T14147 18585-18597 RB denotes respectively
T14148 18597-18598 -RRB- denotes )
T14149 18598-18599 . denotes .
T14150 18600-18603 DT denotes The
T14151 18604-18615 NN denotes calcineurin
T14152 18616-18626 NNS denotes inhibitors
T14153 18627-18638 VBP denotes cyclosporin
T14154 18639-18640 DT denotes A
T14155 18641-18644 CC denotes and
T14156 18645-18650 NNP denotes FK506
T14157 18651-18655 RB denotes also
T14158 18656-18669 VBD denotes downregulated
T14159 18670-18673 DT denotes the
T14160 18674-18679 NNP denotes IL-17
T14161 18680-18689 NN denotes secretion
T14162 18690-18692 RB denotes as
T14163 18693-18697 RB denotes well
T14164 18698-18700 IN denotes as
T14165 18701-18704 DT denotes the
T14166 18705-18722 JJ denotes mitogen-activated
T14167 18723-18730 NN denotes protein
T14168 18731-18737 NN denotes kinase
T14169 18738-18739 -LRB- denotes (
T14170 18739-18743 NNP denotes MAPK
T14171 18743-18744 -RRB- denotes )
T14172 18745-18748 CD denotes p38
T14173 18749-18758 NN denotes inhibitor
T14174 18759-18767 CD denotes SB203580
T14175 18768-18771 VBD denotes did
T14176 18771-18772 , denotes ,
T14177 18773-18780 IN denotes whereas
T14178 18781-18790 NN denotes rapamycin
T14179 18791-18794 CC denotes and
T14180 18795-18802 NNP denotes PD98059
T14181 18803-18806 VBD denotes had
T14182 18807-18809 DT denotes no
T14183 18810-18816 NN denotes effect
T14184 18817-18819 IN denotes on
T14185 18820-18825 NN denotes IL-17
T14186 18826-18832 NNS denotes levels
T14187 18833-18834 -LRB- denotes (
T14188 18834-18838 JJ denotes Fig.
T14189 18839-18840 CD denotes 3
T14190 18840-18841 -RRB- denotes )
T14191 18841-18842 . denotes .
T14192 18843-18845 TO denotes To
T14193 18846-18854 VB denotes evaluate
T14194 18855-18858 DT denotes the
T14195 18859-18870 NN denotes possibility
T14196 18871-18873 IN denotes of
T14197 18874-18886 JJ denotes non-specific
T14198 18887-18897 NN denotes inhibition
T14199 18898-18900 IN denotes by
T14200 18901-18904 DT denotes the
T14201 18905-18909 NN denotes drug
T14202 18910-18912 IN denotes at
T14203 18913-18917 JJ denotes high
T14204 18918-18932 NNS denotes concentrations
T14205 18932-18933 , denotes ,
T14206 18934-18936 PRP denotes we
T14207 18937-18945 VBD denotes observed
T14208 18946-18949 DT denotes the
T14209 18950-18954 NN denotes dose
T14210 18955-18963 NN denotes response
T14211 18964-18966 IN denotes of
T14212 18967-18971 NNP denotes PDTC
T14213 18972-18975 CC denotes and
T14214 18976-18984 NNP denotes LY294002
T14215 18985-18988 IN denotes for
T14216 18989-18992 DT denotes the
T14217 18993-19003 NN denotes inhibition
T14218 19004-19006 IN denotes of
T14219 19007-19012 JJ denotes IL-17
T14220 19013-19023 NN denotes production
T14221 19024-19026 IN denotes in
T14222 19027-19031 NNP denotes PBMC
T14223 19031-19032 . denotes .
T14224 19033-19038 EX denotes There
T14225 19039-19043 VBD denotes were
T14226 19044-19058 JJ denotes dose-dependent
T14227 19059-19070 NNS denotes inhibitions
T14228 19071-19073 IN denotes of
T14229 19074-19079 JJ denotes IL-17
T14230 19080-19090 NN denotes production
T14231 19091-19095 IN denotes with
T14232 19096-19104 NN denotes chemical
T14233 19105-19115 NNS denotes inhibitors
T14234 19116-19117 -LRB- denotes (
T14235 19117-19121 NNP denotes Fig.
T14236 19122-19124 CD denotes 4a
T14237 19124-19125 -RRB- denotes )
T14238 19125-19126 . denotes .
T14239 19127-19130 DT denotes The
T14240 19131-19136 JJ denotes other
T14241 19137-19147 NNS denotes inhibitors
T14242 19148-19150 IN denotes in
T14243 19151-19159 NN denotes addition
T14244 19160-19162 TO denotes to
T14245 19163-19167 NNP denotes PDTC
T14246 19168-19171 CC denotes and
T14247 19172-19180 NNP denotes LY294002
T14248 19181-19187 VBD denotes showed
T14249 19188-19191 DT denotes the
T14250 19192-19196 JJ denotes same
T14251 19197-19204 NN denotes pattern
T14252 19205-19207 IN denotes of
T14253 19208-19218 NN denotes inhibition
T14254 19218-19219 . denotes .
T14255 19220-19229 JJ denotes Cytotoxic
T14256 19230-19237 NNS denotes effects
T14257 19238-19240 IN denotes on
T14258 19241-19245 NNP denotes PBMC
T14259 19246-19248 IN denotes by
T14260 19249-19252 DT denotes the
T14261 19253-19261 NN denotes chemical
T14262 19262-19272 NNS denotes inhibitors
T14263 19273-19275 IN denotes at
T14264 19276-19288 JJ denotes experimental
T14265 19289-19303 NNS denotes concentrations
T14266 19304-19308 VBD denotes were
T14267 19309-19312 RB denotes not
T14268 19313-19321 JJ denotes observed
T14269 19322-19323 -LRB- denotes (
T14270 19323-19327 NNP denotes Fig.
T14271 19328-19330 CD denotes 4b
T14272 19330-19331 -RRB- denotes )
T14273 19331-19332 . denotes .
T14613 19334-19339 NNP denotes IL-17
T14614 19340-19344 NNP denotes mRNA
T14615 19345-19355 NN denotes expression
T14616 19356-19358 IN denotes in
T14617 19359-19361 NNP denotes RA
T14618 19362-19366 NNP denotes PBMC
T14619 19367-19369 TO denotes To
T14620 19370-19373 VB denotes see
T14621 19374-19381 IN denotes whether
T14622 19382-19390 JJ denotes enhanced
T14623 19391-19396 NN denotes IL-17
T14624 19397-19407 NN denotes production
T14625 19408-19413 MD denotes could
T14626 19414-19416 VB denotes be
T14627 19417-19426 VBN denotes regulated
T14628 19427-19429 IN denotes at
T14629 19430-19431 DT denotes a
T14630 19432-19447 JJ denotes transcriptional
T14631 19448-19453 NN denotes level
T14632 19453-19454 , denotes ,
T14633 19455-19472 JJ denotes semi-quantatitive
T14634 19473-19480 NN denotes reverse
T14635 19481-19494 NN denotes transcription
T14636 19495-19505 NN denotes polymerase
T14637 19506-19511 NN denotes chain
T14638 19512-19520 NN denotes reaction
T14639 19521-19524 VBD denotes was
T14640 19525-19534 VBN denotes performed
T14641 19534-19535 . denotes .
T14642 19536-19538 PRP denotes We
T14643 19539-19547 VBD denotes observed
T14644 19548-19549 DT denotes a
T14645 19550-19564 JJ denotes dose-dependent
T14646 19565-19573 NN denotes increase
T14647 19574-19576 IN denotes in
T14648 19577-19582 NNP denotes IL-17
T14649 19583-19587 NNP denotes mRNA
T14650 19588-19599 NNS denotes transcripts
T14651 19600-19605 IN denotes after
T14652 19606-19617 NN denotes stimulation
T14653 19618-19622 IN denotes with
T14654 19623-19631 JJ denotes anti-CD3
T14655 19631-19632 : denotes ;
T14656 19633-19637 DT denotes this
T14657 19638-19641 VBD denotes was
T14658 19642-19651 VBN denotes inhibited
T14659 19652-19654 IN denotes by
T14660 19655-19658 DT denotes the
T14661 19659-19663 NNP denotes PI3K
T14662 19664-19673 NN denotes inhibitor
T14663 19674-19682 NNP denotes LY294002
T14664 19683-19686 CC denotes and
T14665 19687-19689 IN denotes by
T14666 19690-19693 DT denotes the
T14667 19694-19699 JJ denotes NF-κB
T14668 19700-19709 NN denotes inhibitor
T14669 19710-19714 NNP denotes PDTC
T14670 19715-19716 -LRB- denotes (
T14671 19716-19720 NNP denotes Fig.
T14672 19721-19722 CD denotes 5
T14673 19722-19723 -RRB- denotes )
T14674 19723-19724 . denotes .
T15245 19726-19736 NNP denotes Activation
T15246 19737-19739 IN denotes of
T15247 19740-19748 NNP denotes PI3K/Akt
T15248 19749-19755 NN denotes signal
T15249 19756-19768 NN denotes transduction
T15250 19769-19776 NN denotes pathway
T15251 19777-19779 IN denotes on
T15252 19780-19785 NN denotes IL-17
T15253 19786-19796 NN denotes production
T15254 19797-19799 IN denotes by
T15255 19800-19808 JJ denotes anti-CD3
T15256 19809-19811 TO denotes To
T15257 19812-19821 VB denotes determine
T15258 19822-19832 JJ denotes downstream
T15259 19833-19841 NN denotes effector
T15260 19842-19851 NNS denotes molecules
T15261 19852-19854 IN denotes of
T15262 19855-19858 DT denotes the
T15263 19859-19863 NNP denotes PI3K
T15264 19864-19871 NN denotes pathway
T15265 19871-19872 , denotes ,
T15266 19873-19875 PRP denotes we
T15267 19876-19885 VBD denotes evaluated
T15268 19886-19889 DT denotes the
T15269 19890-19900 NN denotes activation
T15270 19901-19903 IN denotes of
T15271 19904-19907 NNP denotes Akt
T15272 19908-19910 IN denotes by
T15273 19911-19918 JJ denotes western
T15274 19919-19927 VBG denotes blotting
T15275 19927-19928 . denotes .
T15276 19929-19931 IN denotes As
T15277 19932-19937 VBN denotes shown
T15278 19938-19940 IN denotes in
T15279 19941-19945 NNP denotes Fig.
T15280 19946-19947 CD denotes 6
T15281 19947-19948 , denotes ,
T15282 19949-19951 IN denotes at
T15283 19952-19954 CD denotes 10
T15284 19955-19958 NN denotes min
T15285 19959-19961 IN denotes of
T15286 19962-19972 NN denotes incubation
T15287 19973-19977 IN denotes with
T15288 19978-19986 JJ denotes anti-CD3
T15289 19987-19988 -LRB- denotes (
T15290 19988-19990 CD denotes 10
T15291 19991-19993 NN denotes μg
T15292 19993-19994 NN denotes /
T15293 19994-19996 NN denotes ml
T15294 19996-19997 -RRB- denotes )
T15295 19998-20000 CC denotes or
T15296 20001-20009 NNP denotes LY294002
T15297 20010-20011 -LRB- denotes (
T15298 20011-20013 CD denotes 20
T15299 20014-20016 NNP denotes μM
T15300 20016-20017 -RRB- denotes )
T15301 20017-20018 , denotes ,
T15302 20019-20021 DT denotes no
T15303 20022-20032 NN denotes difference
T15304 20033-20035 IN denotes in
T15305 20036-20039 DT denotes the
T15306 20040-20047 NNS denotes amounts
T15307 20048-20050 IN denotes of
T15308 20051-20065 VBN denotes phosphorylated
T15309 20066-20069 NNP denotes Akt
T15310 20070-20073 VBD denotes was
T15311 20074-20082 VBN denotes observed
T15312 20082-20083 . denotes .
T15313 20084-20091 RB denotes However
T15314 20091-20092 , denotes ,
T15315 20093-20098 IN denotes after
T15316 20099-20101 CD denotes 30
T15317 20102-20105 NN denotes min
T15318 20106-20108 IN denotes of
T15319 20109-20119 NN denotes incubation
T15320 20119-20120 , denotes ,
T15321 20121-20135 VBN denotes phosphorylated
T15322 20136-20139 NNP denotes Akt
T15323 20140-20149 VBD denotes increased
T15324 20150-20151 -LRB- denotes (
T15325 20151-20155 NN denotes lane
T15326 20156-20157 CD denotes 2
T15327 20157-20158 -RRB- denotes )
T15328 20158-20159 , denotes ,
T15329 20160-20163 CC denotes and
T15330 20164-20167 DT denotes the
T15331 20168-20174 NN denotes effect
T15332 20175-20177 IN denotes of
T15333 20178-20188 NN denotes inhibition
T15334 20189-20191 IN denotes by
T15335 20192-20200 NNP denotes LY294002
T15336 20201-20202 -LRB- denotes (
T15337 20202-20206 NN denotes lane
T15338 20207-20208 CD denotes 3
T15339 20208-20209 -RRB- denotes )
T15340 20210-20217 VBD denotes reached
T15341 20218-20219 DT denotes a
T15342 20220-20224 NN denotes peak
T15343 20225-20227 IN denotes at
T15344 20228-20230 CD denotes 60
T15345 20231-20234 NN denotes min
T15346 20234-20235 , denotes ,
T15347 20236-20243 VBG denotes lasting
T15348 20244-20246 TO denotes to
T15349 20247-20250 CD denotes 120
T15350 20251-20254 CD denotes 240
T15351 20255-20258 NN denotes min
T15352 20258-20259 . denotes .
T15353 20260-20262 IN denotes In
T15354 20263-20271 NN denotes contrast
T15355 20271-20272 , denotes ,
T15356 20273-20291 JJ denotes non-phosphorylated
T15357 20292-20295 NNP denotes Akt
T15358 20296-20299 CC denotes and
T15359 20300-20307 JJ denotes β-actin
T15360 20308-20316 VBD denotes remained
T15361 20317-20326 JJ denotes unchanged
T15362 20327-20337 RB denotes regardless
T15363 20338-20340 IN denotes of
T15364 20341-20351 NN denotes incubation
T15365 20352-20356 NN denotes time
T15366 20356-20357 . denotes .
T15367 20358-20361 NNP denotes PHA
T15368 20361-20362 , denotes ,
T15369 20363-20375 NN denotes concanavalin
T15370 20376-20377 DT denotes A
T15371 20378-20381 CC denotes and
T15372 20382-20387 NNP denotes IL-15
T15373 20388-20392 RB denotes also
T15374 20393-20405 VBD denotes demonstrated
T15375 20406-20409 DT denotes the
T15376 20410-20414 JJ denotes same
T15377 20415-20421 NN denotes effect
T15378 20422-20424 IN denotes on
T15379 20425-20439 VBN denotes phosphorylated
T15380 20440-20443 NNP denotes Akt
T15381 20444-20446 IN denotes as
T15382 20447-20452 VBN denotes shown
T15383 20453-20457 IN denotes with
T15384 20458-20466 JJ denotes anti-CD3
T15385 20466-20467 , denotes ,
T15386 20468-20473 WDT denotes which
T15387 20474-20477 VBD denotes was
T15388 20478-20480 DT denotes an
T15389 20481-20491 NN denotes inhibition
T15390 20492-20494 IN denotes by
T15391 20495-20505 NN denotes wortmannin
T15392 20506-20509 CC denotes and
T15393 20510-20514 NNP denotes PDTC
T15394 20515-20517 RB denotes as
T15395 20518-20522 RB denotes well
T15396 20523-20525 IN denotes as
T15397 20526-20528 IN denotes by
T15398 20529-20537 NNP denotes LY294002
T15399 20538-20539 -LRB- denotes (
T15400 20539-20543 NNS denotes data
T15401 20544-20547 RB denotes not
T15402 20548-20553 VBN denotes shown
T15403 20553-20554 -RRB- denotes )
T15404 20554-20555 . denotes .
T16398 20557-20567 NNP denotes Activation
T16399 20568-20570 IN denotes of
T16400 20571-20574 DT denotes the
T16401 20575-20580 NN denotes NF-κB
T16402 20581-20584 CC denotes and
T16403 20585-20594 NN denotes activator
T16404 20595-20604 NN denotes protein-1
T16405 20605-20606 -LRB- denotes (
T16406 20606-20610 NN denotes AP-1
T16407 20610-20611 -RRB- denotes )
T16408 20612-20619 NN denotes pathway
T16409 20620-20622 IN denotes in
T16410 20623-20626 DT denotes the
T16411 20627-20632 NNP denotes IL-17
T16412 20633-20641 NN denotes promoter
T16413 20642-20648 NN denotes region
T16414 20649-20651 TO denotes To
T16415 20652-20663 VB denotes investigate
T16416 20664-20671 RB denotes further
T16417 20672-20675 DT denotes the
T16418 20676-20689 JJ denotes intracellular
T16419 20690-20699 VBG denotes signaling
T16420 20700-20707 NN denotes pathway
T16421 20708-20717 VBN denotes activated
T16422 20718-20720 IN denotes by
T16423 20721-20729 JJ denotes anti-CD3
T16424 20730-20734 CC denotes plus
T16425 20735-20744 JJ denotes anti-CD28
T16426 20744-20745 , denotes ,
T16427 20746-20758 JJ denotes concanavalin
T16428 20759-20760 DT denotes A
T16429 20760-20761 , denotes ,
T16430 20762-20765 NNP denotes PHA
T16431 20766-20769 CC denotes and
T16432 20770-20775 NNP denotes IL-15
T16433 20775-20776 , denotes ,
T16434 20777-20780 CC denotes and
T16435 20781-20792 JJ denotes responsible
T16436 20793-20796 IN denotes for
T16437 20797-20805 VBG denotes inducing
T16438 20806-20811 NNP denotes IL-17
T16439 20812-20822 NN denotes expression
T16440 20822-20823 , denotes ,
T16441 20824-20826 PRP denotes we
T16442 20827-20836 VBD denotes performed
T16443 20837-20839 DT denotes an
T16444 20840-20855 JJ denotes electrophoretic
T16445 20856-20870 NN denotes mobility-shift
T16446 20871-20876 NN denotes assay
T16447 20877-20878 -LRB- denotes (
T16448 20878-20882 NNP denotes EMSA
T16449 20882-20883 -RRB- denotes )
T16450 20884-20886 IN denotes of
T16451 20887-20892 NN denotes NF-κB
T16452 20893-20904 NN denotes recognition
T16453 20905-20910 NNS denotes sites
T16454 20911-20913 IN denotes in
T16455 20914-20917 DT denotes the
T16456 20918-20927 NNS denotes promoters
T16457 20928-20930 IN denotes of
T16458 20931-20936 NNP denotes IL-17
T16459 20936-20937 . denotes .
T16460 20938-20940 IN denotes As
T16461 20941-20946 VBN denotes shown
T16462 20947-20949 IN denotes in
T16463 20950-20954 NNP denotes Fig.
T16464 20955-20957 CD denotes 7a
T16465 20957-20958 , denotes ,
T16466 20959-20966 JJ denotes nuclear
T16467 20967-20975 NNS denotes extracts
T16468 20976-20980 IN denotes from
T16469 20981-20983 NNP denotes RA
T16470 20984-20988 NNP denotes PBMC
T16471 20989-20999 VBD denotes stimulated
T16472 21000-21004 IN denotes with
T16473 21005-21013 JJ denotes anti-CD3
T16474 21014-21018 CC denotes plus
T16475 21019-21028 JJ denotes anti-CD28
T16476 21029-21030 -LRB- denotes (
T16477 21030-21034 NN denotes lane
T16478 21035-21036 CD denotes 2
T16479 21036-21037 -RRB- denotes )
T16480 21038-21050 VBD denotes demonstrated
T16481 21051-21060 VBN denotes increased
T16482 21061-21068 JJ denotes binding
T16483 21069-21071 IN denotes of
T16484 21072-21077 NN denotes NF-κB
T16485 21078-21080 TO denotes to
T16486 21081-21086 NNP denotes IL-17
T16487 21087-21096 NNS denotes promoters
T16488 21097-21099 IN denotes in
T16489 21100-21110 NN denotes comparison
T16490 21111-21115 IN denotes with
T16491 21116-21120 DT denotes that
T16492 21121-21123 IN denotes of
T16493 21124-21132 NNS denotes controls
T16494 21133-21134 -LRB- denotes (
T16495 21134-21138 NN denotes lane
T16496 21139-21140 CD denotes 1
T16497 21140-21141 -RRB- denotes )
T16498 21141-21142 . denotes .
T16499 21143-21144 DT denotes A
T16500 21145-21155 NN denotes supershift
T16501 21156-21161 NN denotes assay
T16502 21162-21174 VBD denotes demonstrated
T16503 21175-21182 VBN denotes shifted
T16504 21183-21188 NNS denotes bands
T16505 21189-21191 IN denotes in
T16506 21192-21195 CD denotes p65
T16507 21196-21199 CC denotes and
T16508 21200-21203 CD denotes p50
T16509 21204-21205 -LRB- denotes (
T16510 21205-21210 NNS denotes lanes
T16511 21211-21212 CD denotes 3
T16512 21213-21216 CC denotes and
T16513 21217-21218 CD denotes 4
T16514 21218-21219 -RRB- denotes )
T16515 21220-21223 RB denotes not
T16516 21224-21226 IN denotes in
T16517 21227-21232 NNP denotes c-Rel
T16518 21233-21234 -LRB- denotes (
T16519 21234-21238 NN denotes lane
T16520 21239-21240 CD denotes 5
T16521 21240-21241 -RRB- denotes )
T16522 21241-21242 . denotes .
T16523 21243-21245 IN denotes In
T16524 21246-21252 JJ denotes normal
T16525 21253-21257 NNP denotes PBMC
T16526 21258-21261 DT denotes the
T16527 21262-21266 JJ denotes same
T16528 21267-21274 NN denotes pattern
T16529 21275-21278 VBD denotes was
T16530 21279-21287 VBN denotes observed
T16531 21287-21288 , denotes ,
T16532 21289-21292 CC denotes but
T16533 21293-21296 DT denotes the
T16534 21297-21303 NN denotes degree
T16535 21304-21306 IN denotes of
T16536 21307-21312 JJ denotes NF-κB
T16537 21313-21323 NN denotes activation
T16538 21324-21326 IN denotes by
T16539 21327-21335 JJ denotes anti-CD3
T16540 21336-21340 CC denotes plus
T16541 21341-21350 JJ denotes anti-CD28
T16542 21351-21354 VBD denotes was
T16543 21355-21359 JJR denotes less
T16544 21360-21367 JJ denotes intense
T16545 21368-21372 IN denotes than
T16546 21373-21377 DT denotes that
T16547 21378-21380 IN denotes in
T16548 21381-21383 NNP denotes RA
T16549 21384-21388 NNP denotes PBMC
T16550 21389-21390 -LRB- denotes (
T16551 21390-21394 NNP denotes Fig.
T16552 21395-21397 CD denotes 7b
T16553 21397-21398 -RRB- denotes )
T16554 21398-21399 . denotes .
T16555 21400-21402 TO denotes To
T16556 21403-21410 VB denotes confirm
T16557 21411-21414 DT denotes the
T16558 21415-21419 NN denotes link
T16559 21420-21427 IN denotes between
T16560 21428-21432 JJ denotes PI3K
T16561 21433-21441 NN denotes activity
T16562 21442-21445 CC denotes and
T16563 21446-21451 NN denotes NF-κB
T16564 21451-21452 , denotes ,
T16565 21453-21455 PRP denotes we
T16566 21456-21465 VBD denotes performed
T16567 21466-21470 NNP denotes EMSA
T16568 21471-21473 TO denotes to
T16569 21474-21483 VB denotes determine
T16570 21484-21487 DT denotes the
T16571 21488-21493 JJ denotes NF-κB
T16572 21494-21501 JJ denotes binding
T16573 21502-21510 NN denotes activity
T16574 21511-21516 IN denotes after
T16575 21517-21526 NN denotes treatment
T16576 21527-21531 IN denotes with
T16577 21532-21536 DT denotes both
T16578 21537-21545 CD denotes LY294002
T16579 21546-21549 CC denotes and
T16580 21550-21554 NNP denotes PDTC
T16581 21554-21555 . denotes .
T16582 21556-21560 DT denotes Both
T16583 21561-21567 NNS denotes agents
T16584 21568-21573 NN denotes block
T16585 21574-21579 NNP denotes NF-κB
T16586 21580-21591 JJ denotes DNA-binding
T16587 21592-21600 NN denotes activity
T16588 21601-21603 IN denotes in
T16589 21604-21607 DT denotes the
T16590 21608-21613 NNP denotes IL-17
T16591 21614-21622 NN denotes promoter
T16592 21623-21624 -LRB- denotes (
T16593 21624-21628 NNP denotes Fig.
T16594 21629-21631 CD denotes 7c
T16595 21631-21632 -RRB- denotes )
T16596 21632-21633 . denotes .
T16597 21634-21641 JJ denotes Western
T16598 21642-21650 VBG denotes blotting
T16599 21651-21654 IN denotes for
T16600 21655-21660 JJ denotes IκB-α
T16601 21661-21667 VBD denotes showed
T16602 21668-21678 NN denotes inhibition
T16603 21679-21681 IN denotes of
T16604 21682-21693 NN denotes degradation
T16605 21694-21696 IN denotes of
T16606 21697-21702 JJ denotes IκB-α
T16607 21703-21705 IN denotes by
T16608 21706-21714 NNP denotes LY294002
T16609 21715-21718 CC denotes and
T16610 21719-21723 NNP denotes PDTC
T16611 21724-21726 IN denotes at
T16612 21727-21730 DT denotes the
T16613 21731-21735 JJ denotes same
T16614 21736-21740 NN denotes time
T16615 21741-21742 -LRB- denotes (
T16616 21742-21746 NNP denotes Fig.
T16617 21747-21749 CD denotes 7c
T16618 21749-21750 -RRB- denotes )
T16619 21750-21751 . denotes .
T16620 21752-21754 IN denotes In
T16621 21755-21763 NN denotes contrast
T16622 21763-21764 , denotes ,
T16623 21765-21768 DT denotes the
T16624 21769-21773 NNP denotes AP-1
T16625 21774-21781 NN denotes pathway
T16626 21782-21785 VBD denotes was
T16627 21786-21789 RB denotes not
T16628 21790-21799 VBN denotes activated
T16629 21800-21802 IN denotes by
T16630 21803-21814 NN denotes stimulation
T16631 21815-21819 IN denotes with
T16632 21820-21828 JJ denotes anti-CD3
T16633 21829-21833 CC denotes plus
T16634 21834-21843 JJ denotes anti-CD28
T16635 21844-21845 -LRB- denotes (
T16636 21845-21849 NNS denotes data
T16637 21850-21853 RB denotes not
T16638 21854-21859 VBN denotes shown
T16639 21859-21860 -RRB- denotes )
T16640 21860-21861 , denotes ,
T16641 21862-21875 VBG denotes demonstrating
T16642 21876-21880 IN denotes that
T16643 21881-21886 NNP denotes NF-κB
T16644 21887-21889 VBZ denotes is
T16645 21890-21893 DT denotes the
T16646 21894-21898 JJ denotes main
T16647 21899-21912 JJ denotes intracellular
T16648 21913-21922 VBG denotes signaling
T16649 21923-21930 NN denotes pathway
T16650 21931-21933 IN denotes in
T16651 21934-21939 JJ denotes IL-17
T16652 21940-21950 NN denotes production
T16653 21951-21953 IN denotes by
T16654 21954-21963 VBN denotes activated
T16655 21964-21968 NNP denotes PBMC
T16656 21969-21973 IN denotes from
T16657 21974-21982 NNS denotes patients
T16658 21983-21987 IN denotes with
T16659 21988-21990 NNP denotes RA
T16660 21990-21991 . denotes .
T20377 22004-22009 NNP denotes IL-17
T20378 22010-22013 VBD denotes was
T20379 22014-22019 JJ denotes first
T20380 22020-22029 VBN denotes described
T20381 22030-22032 IN denotes as
T20382 22033-22034 DT denotes a
T20383 22035-22036 NNP denotes T
T20384 22037-22041 NN denotes cell
T20385 22042-22049 NN denotes product
T20386 22050-22054 IN denotes with
T20387 22055-22070 JJ denotes proinflammatory
T20388 22071-22081 NNS denotes properties
T20389 22082-22083 NNP denotes [
T20390 22083-22087 CD denotes 5,22
T20391 22087-22088 NNP denotes ]
T20392 22088-22089 . denotes .
T20393 22090-22092 NNP denotes RA
T20394 22093-22095 VBZ denotes is
T20395 22096-22109 VBN denotes characterized
T20396 22110-22112 IN denotes by
T20397 22113-22124 NN denotes hyperplasia
T20398 22125-22127 IN denotes of
T20399 22128-22136 JJ denotes synovial
T20400 22137-22143 NN denotes lining
T20401 22144-22149 NNS denotes cells
T20402 22150-22153 CC denotes and
T20403 22154-22156 DT denotes an
T20404 22157-22164 JJ denotes intense
T20405 22165-22177 NN denotes infiltration
T20406 22178-22180 IN denotes by
T20407 22181-22192 NN denotes mononuclear
T20408 22193-22198 NNS denotes cells
T20409 22199-22200 NNP denotes [
T20410 22200-22202 CD denotes 23
T20411 22202-22203 NNP denotes ]
T20412 22203-22204 . denotes .
T20413 22205-22220 JJ denotes Proinflammatory
T20414 22221-22230 NNS denotes cytokines
T20415 22231-22235 JJ denotes such
T20416 22236-22238 IN denotes as
T20417 22239-22243 NN denotes IL-1
T20418 22244-22247 CC denotes and
T20419 22248-22253 NN denotes TNF-α
T20420 22254-22257 VBP denotes are
T20421 22258-22266 JJ denotes abundant
T20422 22267-22269 IN denotes in
T20423 22270-22280 JJ denotes rheumatoid
T20424 22281-22289 NN denotes synovium
T20425 22289-22290 , denotes ,
T20426 22291-22298 IN denotes whereas
T20427 22299-22302 DT denotes the
T20428 22303-22304 NNP denotes T
T20429 22305-22317 JJ denotes cell-derived
T20430 22318-22327 NNS denotes cytokines
T20431 22327-22328 , denotes ,
T20432 22329-22339 RB denotes especially
T20433 22340-22344 NN denotes IL-4
T20434 22345-22348 CC denotes and
T20435 22349-22361 NN denotes interferon-γ
T20436 22361-22362 , denotes ,
T20437 22363-22367 VBP denotes have
T20438 22368-22373 RB denotes often
T20439 22374-22380 VBN denotes proved
T20440 22381-22390 JJ denotes difficult
T20441 22391-22393 TO denotes to
T20442 22394-22400 VB denotes detect
T20443 22401-22403 IN denotes in
T20444 22404-22406 NNP denotes RA
T20445 22407-22415 NN denotes synovium
T20446 22416-22417 NNP denotes [
T20447 22417-22419 CD denotes 24
T20448 22419-22420 NNP denotes ]
T20449 22420-22421 . denotes .
T20450 22422-22430 IN denotes Although
T20451 22431-22432 NNP denotes T
T20452 22433-22438 NNS denotes cells
T20453 22439-22442 MD denotes may
T20454 22443-22447 VB denotes have
T20455 22448-22449 DT denotes a
T20456 22450-22454 NN denotes role
T20457 22455-22457 IN denotes in
T20458 22458-22461 DT denotes the
T20459 22462-22474 NN denotes augmentation
T20460 22475-22477 IN denotes of
T20461 22478-22488 JJ denotes rheumatoid
T20462 22489-22497 JJ denotes synovial
T20463 22498-22510 NN denotes inflammation
T20464 22510-22511 , denotes ,
T20465 22512-22515 DT denotes the
T20466 22516-22520 NN denotes lack
T20467 22521-22523 IN denotes of
T20468 22524-22525 NNP denotes T
T20469 22526-22538 JJ denotes cell-derived
T20470 22539-22548 NNS denotes cytokines
T20471 22549-22552 VBZ denotes has
T20472 22553-22560 VBN denotes limited
T20473 22561-22564 PRP$ denotes its
T20474 22565-22575 NN denotes importance
T20475 22575-22576 . denotes .
T20476 22577-22579 IN denotes In
T20477 22580-22584 DT denotes this
T20478 22585-22592 NN denotes respect
T20479 22592-22593 , denotes ,
T20480 22594-22599 NNP denotes IL-17
T20481 22600-22602 VBZ denotes is
T20482 22603-22612 VBG denotes appealing
T20483 22613-22620 IN denotes because
T20484 22621-22623 PRP denotes it
T20485 22624-22627 VBZ denotes has
T20486 22628-22632 VBN denotes been
T20487 22633-22642 VBN denotes described
T20488 22643-22645 IN denotes as
T20489 22646-22647 DT denotes a
T20490 22648-22649 NNP denotes T
T20491 22650-22662 JJ denotes cell-derived
T20492 22663-22671 NN denotes cytokine
T20493 22672-22676 IN denotes with
T20494 22677-22692 JJ denotes proinflammatory
T20495 22693-22703 NNS denotes properties
T20496 22703-22704 . denotes .
T20497 22705-22707 IN denotes In
T20498 22708-22711 PRP$ denotes our
T20499 22712-22719 NNS denotes studies
T20500 22719-22720 , denotes ,
T20501 22721-22723 PRP denotes we
T20502 22724-22729 VBD denotes tried
T20503 22730-22732 TO denotes to
T20504 22733-22741 VB denotes evaluate
T20505 22742-22745 WRB denotes how
T20506 22746-22751 JJ denotes IL-17
T20507 22752-22762 NN denotes production
T20508 22763-22765 VBZ denotes is
T20509 22766-22775 VBN denotes regulated
T20510 22776-22778 IN denotes in
T20511 22779-22781 NNP denotes RA
T20512 22782-22786 NNP denotes PBMC
T20513 22786-22787 , denotes ,
T20514 22788-22791 CC denotes and
T20515 22792-22797 WDT denotes which
T20516 22798-22807 VBG denotes signaling
T20517 22808-22815 NN denotes pathway
T20518 22816-22818 PRP denotes it
T20519 22819-22823 VBD denotes used
T20520 22823-22824 . denotes .
T20521 22825-22831 NNS denotes Levels
T20522 22832-22834 IN denotes of
T20523 22835-22840 NNP denotes IL-17
T20524 22841-22845 VBD denotes were
T20525 22846-22851 VBN denotes found
T20526 22852-22854 TO denotes to
T20527 22855-22857 VB denotes be
T20528 22858-22864 JJR denotes higher
T20529 22865-22867 IN denotes in
T20530 22868-22870 NNP denotes RA
T20531 22871-22879 JJ denotes synovial
T20532 22880-22885 NN denotes fluid
T20533 22886-22890 IN denotes than
T20534 22891-22893 IN denotes in
T20535 22894-22896 NNP denotes OA
T20536 22897-22905 JJ denotes synovial
T20537 22906-22911 NN denotes fluid
T20538 22912-22913 NNP denotes [
T20539 22913-22915 CD denotes 15
T20540 22915-22916 NNP denotes ]
T20541 22916-22917 . denotes .
T20542 22918-22925 RB denotes However
T20543 22925-22926 , denotes ,
T20544 22927-22932 EX denotes there
T20545 22933-22936 VBP denotes are
T20546 22937-22940 JJ denotes few
T20547 22941-22945 NNS denotes data
T20548 22946-22955 JJ denotes available
T20549 22956-22958 IN denotes on
T20550 22959-22962 DT denotes the
T20551 22963-22969 NNS denotes agents
T20552 22970-22974 WDT denotes that
T20553 22975-22984 VBP denotes stimulate
T20554 22985-22990 JJ denotes IL-17
T20555 22991-23001 NN denotes production
T20556 23002-23004 IN denotes in
T20557 23005-23007 NNP denotes RA
T20558 23007-23008 , denotes ,
T20559 23009-23017 IN denotes although
T20560 23018-23021 DT denotes the
T20561 23022-23029 JJS denotes highest
T20562 23030-23035 NN denotes level
T20563 23036-23038 IN denotes of
T20564 23039-23044 JJ denotes IL-17
T20565 23045-23055 NN denotes production
T20566 23056-23059 MD denotes can
T20567 23060-23062 VB denotes be
T20568 23063-23071 VBN denotes achieved
T20569 23072-23074 IN denotes by
T20570 23075-23083 JJ denotes anti-CD3
T20571 23083-23084 NN denotes /
T20572 23084-23093 JJ denotes anti-CD28
T20573 23094-23105 NN denotes stimulation
T20574 23106-23108 IN denotes in
T20575 23109-23116 JJ denotes healthy
T20576 23117-23128 NNS denotes individuals
T20577 23129-23130 NNP denotes [
T20578 23130-23132 CD denotes 25
T20579 23132-23133 NNP denotes ]
T20580 23133-23134 . denotes .
T20581 23135-23137 IN denotes In
T20582 23138-23141 PRP$ denotes our
T20583 23142-23153 NNS denotes experiments
T20584 23153-23154 , denotes ,
T20585 23155-23158 NNP denotes PHA
T20586 23159-23161 IN denotes as
T20587 23162-23170 NNS denotes mitogens
T20588 23170-23171 , denotes ,
T20589 23172-23174 RB denotes as
T20590 23175-23179 RB denotes well
T20591 23180-23182 IN denotes as
T20592 23183-23191 JJ denotes anti-CD3
T20593 23191-23192 NN denotes /
T20594 23192-23201 NN denotes anti-CD28
T20595 23202-23205 IN denotes for
T20596 23206-23215 VBG denotes signaling
T20597 23216-23223 IN denotes through
T20598 23224-23227 DT denotes the
T20599 23228-23229 NNP denotes T
T20600 23230-23234 NN denotes cell
T20601 23235-23243 NN denotes receptor
T20602 23243-23244 , denotes ,
T20603 23245-23254 VBD denotes increased
T20604 23255-23260 NNP denotes IL-17
T20605 23261-23271 NN denotes production
T20606 23272-23276 IN denotes from
T20607 23277-23279 NNP denotes RA
T20608 23280-23284 NNP denotes PBMC
T20609 23285-23287 IN denotes in
T20610 23288-23289 DT denotes a
T20611 23290-23304 JJ denotes dose-dependent
T20612 23305-23311 NN denotes manner
T20613 23311-23312 . denotes .
T20614 23313-23315 PRP denotes We
T20615 23316-23321 VBD denotes found
T20616 23321-23322 , denotes ,
T20617 23323-23325 IN denotes by
T20618 23326-23327 DT denotes a
T20619 23328-23332 NN denotes cell
T20620 23333-23346 NN denotes proliferation
T20621 23347-23352 NN denotes assay
T20622 23353-23354 -LRB- denotes (
T20623 23354-23358 NNS denotes data
T20624 23359-23362 RB denotes not
T20625 23363-23368 VBN denotes shown
T20626 23368-23369 -RRB- denotes )
T20627 23369-23370 , denotes ,
T20628 23371-23375 IN denotes that
T20629 23376-23380 DT denotes this
T20630 23381-23393 NN denotes upregulation
T20631 23394-23396 IN denotes of
T20632 23397-23402 NNP denotes IL-17
T20633 23403-23408 MD denotes might
T20634 23409-23411 VB denotes be
T20635 23412-23415 JJ denotes due
T20636 23416-23418 TO denotes to
T20637 23419-23428 VBN denotes increased
T20638 23429-23437 JJ denotes cellular
T20639 23438-23446 NN denotes activity
T20640 23447-23453 RB denotes rather
T20641 23454-23458 IN denotes than
T20642 23459-23461 TO denotes to
T20643 23462-23470 JJ denotes cellular
T20644 23471-23484 NN denotes proliferation
T20645 23484-23485 . denotes .
T20646 23486-23491 NNP denotes IL-17
T20647 23492-23494 VBZ denotes is
T20648 23495-23503 VBN denotes produced
T20649 23504-23510 RB denotes mainly
T20650 23511-23513 IN denotes by
T20651 23514-23523 VBN denotes activated
T20652 23524-23527 NNP denotes CD4
T20653 23527-23528 CD denotes +
T20654 23529-23530 NNP denotes T
T20655 23531-23536 NNS denotes cells
T20656 23536-23537 , denotes ,
T20657 23538-23548 RB denotes especially
T20658 23549-23552 IN denotes for
T20659 23553-23560 JJ denotes Th1/Th0
T20660 23561-23566 NNS denotes cells
T20661 23566-23567 , denotes ,
T20662 23568-23571 RB denotes not
T20663 23572-23575 DT denotes the
T20664 23576-23579 JJ denotes Th2
T20665 23580-23589 NN denotes phenotype
T20666 23590-23591 NNP denotes [
T20667 23591-23593 CD denotes 26
T20668 23593-23594 NNP denotes ]
T20669 23594-23595 . denotes .
T20670 23596-23603 RB denotes However
T20671 23603-23604 , denotes ,
T20672 23605-23607 PRP denotes it
T20673 23608-23611 MD denotes can
T20674 23612-23616 RB denotes also
T20675 23617-23619 VB denotes be
T20676 23620-23628 VBN denotes produced
T20677 23629-23631 IN denotes by
T20678 23632-23635 CD denotes CD8
T20679 23635-23636 CD denotes +
T20680 23637-23638 NNP denotes T
T20681 23639-23644 NNS denotes cells
T20682 23645-23648 IN denotes via
T20683 23649-23651 DT denotes an
T20684 23652-23657 JJ denotes IL-23
T20685 23658-23668 VBG denotes triggering
T20686 23669-23678 NN denotes mechanism
T20687 23679-23681 IN denotes in
T20688 23682-23695 JJ denotes Gram-negative
T20689 23696-23705 JJ denotes pulmonary
T20690 23706-23715 NN denotes infection
T20691 23716-23717 NNP denotes [
T20692 23717-23719 CD denotes 14
T20693 23719-23720 NNP denotes ]
T20694 23720-23721 . denotes .
T20695 23722-23724 IN denotes In
T20696 23725-23733 NN denotes addition
T20697 23733-23734 , denotes ,
T20698 23735-23740 JJ denotes IL-17
T20699 23741-23751 NN denotes production
T20700 23752-23755 VBD denotes was
T20701 23756-23769 RB denotes significantly
T20702 23770-23779 VBN denotes augmented
T20703 23780-23782 IN denotes by
T20704 23783-23784 NNP denotes T
T20705 23785-23790 NNS denotes cells
T20706 23791-23802 VBG denotes recognizing
T20707 23803-23807 NN denotes type
T20708 23808-23810 NNP denotes II
T20709 23811-23819 NN denotes collagen
T20710 23820-23822 IN denotes in
T20711 23823-23824 DT denotes a
T20712 23825-23841 JJ denotes collagen-induced
T20713 23842-23851 NN denotes arthritis
T20714 23852-23857 NN denotes model
T20715 23858-23859 NNP denotes [
T20716 23859-23861 CD denotes 27
T20717 23861-23862 NNP denotes ]
T20718 23862-23863 . denotes .
T20719 23864-23865 DT denotes A
T20720 23866-23873 JJ denotes complex
T20721 23874-23885 NN denotes interaction
T20722 23886-23893 IN denotes between
T20723 23894-23899 NNS denotes cells
T20724 23900-23902 IN denotes in
T20725 23903-23911 JJ denotes inflamed
T20726 23912-23914 NNP denotes RA
T20727 23915-23921 NNS denotes joints
T20728 23922-23927 MD denotes might
T20729 23928-23935 VB denotes produce
T20730 23936-23937 DT denotes a
T20731 23938-23945 NN denotes variety
T20732 23946-23948 IN denotes of
T20733 23949-23964 JJ denotes proinflammatory
T20734 23965-23974 NNS denotes cytokines
T20735 23975-23978 CC denotes and
T20736 23979-23989 NNS denotes chemokines
T20737 23989-23990 , denotes ,
T20738 23991-23996 WDT denotes which
T20739 23997-24001 RB denotes also
T20740 24002-24010 VBP denotes activate
T20741 24011-24016 JJ denotes other
T20742 24017-24022 NNS denotes cells
T20743 24023-24025 IN denotes in
T20744 24026-24029 DT denotes the
T20745 24030-24036 NNS denotes joints
T20746 24036-24037 . denotes .
T20747 24038-24041 IN denotes For
T20748 24042-24049 NN denotes example
T20749 24049-24050 , denotes ,
T20750 24051-24056 NNP denotes IL-17
T20751 24057-24067 VBZ denotes stimulates
T20752 24068-24078 JJ denotes rheumatoid
T20753 24079-24091 NNS denotes synoviocytes
T20754 24092-24094 TO denotes to
T20755 24095-24102 VB denotes secrete
T20756 24103-24110 JJ denotes several
T20757 24111-24120 NNS denotes cytokines
T20758 24121-24125 JJ denotes such
T20759 24126-24128 IN denotes as
T20760 24129-24133 NNP denotes IL-6
T20761 24133-24134 , denotes ,
T20762 24135-24139 NNP denotes IL-8
T20763 24140-24143 CC denotes and
T20764 24144-24149 NN denotes tumor
T20765 24150-24158 NN denotes necrosis
T20766 24159-24176 JJ denotes factor-stimulated
T20767 24177-24181 NN denotes gene
T20768 24182-24183 CD denotes 6
T20769 24184-24186 RB denotes as
T20770 24187-24191 RB denotes well
T20771 24192-24194 IN denotes as
T20772 24195-24208 NN denotes prostaglandin
T20773 24209-24211 NN denotes E2
T20774 24212-24214 IN denotes in
T20775 24215-24220 NN denotes vitro
T20776 24221-24222 NNP denotes [
T20777 24222-24230 CD denotes 12,28,29
T20778 24230-24231 NNP denotes ]
T20779 24231-24232 . denotes .
T20780 24233-24238 EX denotes There
T20781 24239-24242 VBP denotes are
T20782 24243-24245 RB denotes as
T20783 24246-24249 RB denotes yet
T20784 24250-24253 JJ denotes few
T20785 24254-24258 NNS denotes data
T20786 24259-24268 JJ denotes available
T20787 24269-24271 IN denotes on
T20788 24272-24275 DT denotes the
T20789 24276-24282 NNS denotes agents
T20790 24283-24287 WDT denotes that
T20791 24288-24297 VBP denotes stimulate
T20792 24298-24303 JJ denotes IL-17
T20793 24304-24314 NN denotes production
T20794 24315-24317 IN denotes in
T20795 24318-24320 NNP denotes RA
T20796 24320-24321 , denotes ,
T20797 24322-24330 IN denotes although
T20798 24331-24335 DT denotes some
T20799 24336-24345 NNS denotes cytokines
T20800 24346-24347 -LRB- denotes (
T20801 24347-24352 NN denotes IL-15
T20802 24353-24356 CC denotes and
T20803 24357-24362 NN denotes IL-23
T20804 24362-24363 -RRB- denotes )
T20805 24364-24368 VBP denotes have
T20806 24369-24373 VBN denotes been
T20807 24374-24379 VBN denotes known
T20808 24380-24382 TO denotes to
T20809 24383-24391 VB denotes regulate
T20810 24392-24397 JJ denotes IL-17
T20811 24398-24408 NN denotes production
T20812 24409-24410 NNP denotes [
T20813 24410-24415 CD denotes 13,14
T20814 24415-24416 NNP denotes ]
T20815 24416-24417 . denotes .
T20816 24418-24420 PRP denotes We
T20817 24421-24430 RB denotes therefore
T20818 24431-24443 VBD denotes investigated
T20819 24444-24447 DT denotes the
T20820 24448-24450 IN denotes in
T20821 24451-24456 NN denotes vitro
T20822 24457-24467 NN denotes production
T20823 24468-24470 IN denotes of
T20824 24471-24476 NNP denotes IL-17
T20825 24477-24479 IN denotes in
T20826 24480-24482 NNP denotes RA
T20827 24483-24487 NNP denotes PBMC
T20828 24488-24498 VBG denotes responding
T20829 24499-24501 TO denotes to
T20830 24502-24503 DT denotes a
T20831 24504-24511 NN denotes variety
T20832 24512-24514 IN denotes of
T20833 24515-24535 NNS denotes cytokines/chemokines
T20834 24536-24539 CC denotes and
T20835 24540-24548 NNS denotes mitogens
T20836 24549-24551 RB denotes as
T20837 24552-24556 RB denotes well
T20838 24557-24559 IN denotes as
T20839 24560-24561 NNP denotes T
T20840 24562-24566 NN denotes cell
T20841 24567-24575 NN denotes receptor
T20842 24576-24577 -LRB- denotes (
T20843 24577-24580 NNP denotes TCR
T20844 24580-24581 -RRB- denotes )
T20845 24582-24590 NN denotes ligation
T20846 24591-24596 VBG denotes using
T20847 24597-24605 JJ denotes anti-CD3
T20848 24605-24606 NN denotes /
T20849 24606-24615 NN denotes anti-CD28
T20850 24615-24616 . denotes .
T20851 24617-24620 PRP$ denotes Our
T20852 24621-24628 NNS denotes studies
T20853 24629-24641 VBD denotes demonstrated
T20854 24642-24646 IN denotes that
T20855 24647-24652 NNP denotes IL-15
T20856 24653-24656 CC denotes and
T20857 24657-24662 NNP denotes MCP-1
T20858 24663-24665 RB denotes as
T20859 24666-24670 RB denotes well
T20860 24671-24673 IN denotes as
T20861 24674-24677 NNP denotes TCR
T20862 24678-24686 NN denotes ligation
T20863 24687-24700 RB denotes significantly
T20864 24701-24710 VBD denotes increased
T20865 24711-24714 DT denotes the
T20866 24715-24725 NN denotes production
T20867 24726-24728 IN denotes of
T20868 24729-24734 NNP denotes IL-17
T20869 24735-24737 IN denotes in
T20870 24738-24740 NNP denotes RA
T20871 24741-24745 NNP denotes PBMC
T20872 24745-24746 . denotes .
T20873 24747-24753 VBG denotes Adding
T20874 24754-24759 NN denotes IL-15
T20875 24760-24762 CC denotes or
T20876 24763-24768 NN denotes MCP-1
T20877 24769-24771 TO denotes to
T20878 24772-24775 NNP denotes TCR
T20879 24776-24784 NN denotes ligation
T20880 24785-24794 VBD denotes augmented
T20881 24795-24800 JJ denotes IL-17
T20882 24801-24811 NN denotes production
T20883 24812-24816 RBR denotes more
T20884 24817-24825 RB denotes markedly
T20885 24825-24826 . denotes .
T20886 24827-24829 IN denotes In
T20887 24830-24838 NN denotes contrast
T20888 24838-24839 , denotes ,
T20889 24840-24844 NNP denotes IL-1
T20890 24845-24848 CC denotes and
T20891 24849-24854 NNP denotes TNF-α
T20892 24854-24855 , denotes ,
T20893 24856-24861 WDT denotes which
T20894 24862-24865 VBP denotes are
T20895 24866-24871 VBN denotes known
T20896 24872-24874 TO denotes to
T20897 24875-24879 VB denotes have
T20898 24880-24895 JJ denotes proinflammatory
T20899 24896-24906 NNS denotes properties
T20900 24907-24910 CC denotes and
T20901 24911-24913 TO denotes to
T20902 24914-24916 VB denotes be
T20903 24917-24926 VBN denotes increased
T20904 24927-24929 IN denotes in
T20905 24930-24932 NNP denotes RA
T20906 24933-24939 NNS denotes joints
T20907 24939-24940 , denotes ,
T20908 24941-24944 VBD denotes did
T20909 24945-24948 RB denotes not
T20910 24949-24955 VB denotes affect
T20911 24956-24961 JJ denotes IL-17
T20912 24962-24972 NN denotes production
T20913 24972-24973 . denotes .
T20914 24974-24977 PRP$ denotes Our
T20915 24978-24982 NNS denotes data
T20916 24983-24987 VBD denotes were
T20917 24988-24998 JJ denotes consistent
T20918 24999-25003 IN denotes with
T20919 25004-25005 DT denotes a
T20920 25006-25012 JJ denotes recent
T20921 25013-25019 NN denotes report
T20922 25020-25024 WDT denotes that
T20923 25025-25030 NNP denotes IL-15
T20924 25031-25040 VBD denotes triggered
T20925 25041-25043 IN denotes in
T20926 25044-25049 NN denotes vitro
T20927 25050-25055 NN denotes IL-17
T20928 25056-25066 NN denotes production
T20929 25067-25069 IN denotes in
T20930 25070-25074 NNP denotes PBMC
T20931 25074-25075 , denotes ,
T20932 25076-25079 CC denotes but
T20933 25080-25085 NNP denotes TNF-α
T20934 25086-25089 VBD denotes did
T20935 25090-25093 RB denotes not
T20936 25094-25096 VB denotes do
T20937 25097-25099 RB denotes so
T20938 25100-25101 JJ denotes [
T20939 25101-25103 CD denotes 13
T20940 25103-25104 NN denotes ]
T20941 25104-25105 . denotes .
T20942 25106-25114 IN denotes Although
T20943 25115-25120 EX denotes there
T20944 25121-25125 VBD denotes were
T20945 25126-25128 DT denotes no
T20946 25129-25133 NNS denotes data
T20947 25134-25138 WDT denotes that
T20948 25139-25144 NNP denotes MCP-1
T20949 25145-25153 RB denotes directly
T20950 25154-25161 VBZ denotes induces
T20951 25162-25163 NNP denotes T
T20952 25164-25168 NN denotes cell
T20953 25169-25179 NN denotes activation
T20954 25179-25180 , denotes ,
T20955 25181-25183 PRP denotes it
T20956 25184-25189 MD denotes might
T20957 25190-25195 VB denotes exert
T20958 25196-25203 NNS denotes effects
T20959 25204-25214 RB denotes indirectly
T20960 25215-25217 IN denotes on
T20961 25218-25219 NNP denotes T
T20962 25220-25225 NNS denotes cells
T20963 25226-25233 IN denotes through
T20964 25234-25237 DT denotes the
T20965 25238-25248 NN denotes activation
T20966 25249-25251 IN denotes of
T20967 25252-25273 NNS denotes monocytes/macrophages
T20968 25274-25276 IN denotes in
T20969 25277-25281 NNP denotes PBMC
T20970 25282-25290 NNS denotes cultures
T20971 25290-25291 . denotes .
T20972 25292-25294 IN denotes As
T20973 25295-25303 VBN denotes reported
T20974 25304-25307 IN denotes for
T20975 25308-25314 JJ denotes normal
T20976 25315-25326 NNS denotes individuals
T20977 25327-25328 NNP denotes [
T20978 25328-25330 CD denotes 25
T20979 25330-25331 NNP denotes ]
T20980 25331-25332 , denotes ,
T20981 25333-25334 NNP denotes T
T20982 25335-25339 NN denotes cell
T20983 25340-25350 NN denotes activation
T20984 25351-25358 IN denotes through
T20985 25359-25367 JJ denotes anti-CD3
T20986 25367-25368 NN denotes /
T20987 25368-25377 NN denotes anti-CD28
T20988 25378-25382 RB denotes also
T20989 25383-25392 VBZ denotes increases
T20990 25393-25398 NNP denotes IL-17
T20991 25399-25408 NN denotes induction
T20992 25409-25411 IN denotes in
T20993 25412-25414 NNP denotes RA
T20994 25415-25419 NNP denotes PBMC
T20995 25419-25420 . denotes .
T20996 25421-25429 IN denotes Although
T20997 25430-25433 DT denotes the
T20998 25434-25443 VBG denotes signaling
T20999 25444-25451 NN denotes pathway
T21000 25452-25455 IN denotes for
T21001 25456-25459 DT denotes the
T21002 25460-25469 NN denotes induction
T21003 25470-25472 IN denotes of
T21004 25473-25493 NNS denotes cytokines/chemokines
T21005 25494-25496 IN denotes by
T21006 25497-25502 NNP denotes IL-17
T21007 25503-25506 VBZ denotes has
T21008 25507-25511 VBN denotes been
T21009 25512-25522 VBN denotes documented
T21010 25523-25529 RB denotes widely
T21011 25530-25531 NNP denotes [
T21012 25531-25538 CD denotes 8,30,31
T21013 25538-25539 NNP denotes ]
T21014 25539-25540 , denotes ,
T21015 25541-25543 DT denotes no
T21016 25544-25548 NNS denotes data
T21017 25549-25553 VBP denotes have
T21018 25554-25558 VBN denotes been
T21019 25559-25568 JJ denotes available
T21020 25569-25571 IN denotes on
T21021 25572-25575 WRB denotes how
T21022 25576-25581 JJ denotes IL-17
T21023 25582-25592 NN denotes production
T21024 25593-25596 MD denotes can
T21025 25597-25599 VB denotes be
T21026 25600-25609 VBN denotes regulated
T21027 25610-25612 IN denotes by
T21028 25613-25620 JJ denotes certain
T21029 25621-25630 VBG denotes signaling
T21030 25631-25639 NNS denotes pathways
T21031 25639-25640 . denotes .
T21032 25641-25643 IN denotes By
T21033 25644-25649 VBG denotes using
T21034 25650-25656 NN denotes signal
T21035 25657-25669 NN denotes transduction
T21036 25670-25680 NNS denotes inhibitors
T21037 25680-25681 , denotes ,
T21038 25682-25684 PRP denotes we
T21039 25685-25694 RB denotes therefore
T21040 25695-25703 VBD denotes examined
T21041 25704-25709 WDT denotes which
T21042 25710-25719 VBG denotes signaling
T21043 25720-25727 NN denotes pathway
T21044 25728-25731 VBD denotes was
T21045 25732-25738 RB denotes mainly
T21046 25739-25747 VBN denotes involved
T21047 25748-25750 IN denotes in
T21048 25751-25754 DT denotes the
T21049 25755-25764 NN denotes induction
T21050 25765-25767 IN denotes of
T21051 25768-25773 NNP denotes IL-17
T21052 25774-25776 IN denotes in
T21053 25777-25779 NNP denotes RA
T21054 25780-25784 NNP denotes PBMC
T21055 25784-25785 . denotes .
T21056 25786-25788 PRP denotes We
T21057 25789-25799 VBD denotes identified
T21058 25800-25804 IN denotes that
T21059 25805-25821 JJ denotes anti-CD3-induced
T21060 25822-25827 NN denotes IL-17
T21061 25828-25838 NN denotes production
T21062 25839-25841 IN denotes in
T21063 25842-25844 NNP denotes RA
T21064 25845-25849 NNP denotes PBMC
T21065 25850-25853 VBD denotes was
T21066 25854-25867 RB denotes significantly
T21067 25868-25876 VBN denotes hampered
T21068 25877-25879 IN denotes by
T21069 25880-25883 DT denotes the
T21070 25884-25888 NNP denotes PI3K
T21071 25889-25898 NN denotes inhibitor
T21072 25899-25907 NNP denotes LY294002
T21073 25908-25911 CC denotes and
T21074 25912-25915 DT denotes the
T21075 25916-25921 NNP denotes NF-κB
T21076 25922-25931 NN denotes inhibitor
T21077 25932-25936 NNP denotes PDTC
T21078 25937-25939 TO denotes to
T21079 25940-25950 JJ denotes comparable
T21080 25951-25957 NNS denotes levels
T21081 25958-25960 IN denotes of
T21082 25961-25966 JJ denotes basal
T21083 25967-25977 NN denotes production
T21084 25978-25985 IN denotes without
T21085 25986-25997 NN denotes stimulation
T21086 25997-25998 . denotes .
T21087 25999-26001 PRP denotes We
T21088 26002-26006 RB denotes also
T21089 26007-26012 VBD denotes found
T21090 26013-26017 IN denotes that
T21091 26018-26034 JJ denotes anti-CD3-induced
T21092 26035-26040 NN denotes IL-17
T21093 26041-26051 NN denotes production
T21094 26052-26055 VBD denotes was
T21095 26056-26069 VBN denotes downregulated
T21096 26070-26072 IN denotes by
T21097 26073-26076 DT denotes the
T21098 26077-26085 NN denotes addition
T21099 26086-26088 IN denotes of
T21100 26089-26097 NNP denotes SB203580
T21101 26097-26098 , denotes ,
T21102 26099-26100 DT denotes a
T21103 26101-26104 JJ denotes p38
T21104 26105-26109 NNP denotes MAPK
T21105 26110-26119 NN denotes inhibitor
T21106 26119-26120 . denotes .
T21107 26121-26123 PRP denotes It
T21108 26124-26126 VBZ denotes is
T21109 26127-26138 JJ denotes interesting
T21110 26139-26143 IN denotes that
T21111 26144-26145 DT denotes a
T21112 26146-26152 NN denotes series
T21113 26153-26155 IN denotes of
T21114 26156-26164 NN denotes evidence
T21115 26165-26173 VBZ denotes supports
T21116 26174-26183 NN denotes crosstalk
T21117 26184-26191 IN denotes between
T21118 26192-26197 NNP denotes NF-κB
T21119 26198-26201 CC denotes and
T21120 26202-26205 CD denotes p38
T21121 26205-26206 . denotes .
T21122 26207-26209 IN denotes In
T21123 26210-26218 NNS denotes myocytes
T21124 26218-26219 , denotes ,
T21125 26220-26223 NNP denotes IκB
T21126 26224-26232 NN denotes kinase-β
T21127 26233-26235 VBZ denotes is
T21128 26236-26245 VBN denotes activated
T21129 26246-26248 IN denotes by
T21130 26249-26252 CD denotes p38
T21131 26253-26254 NNP denotes [
T21132 26254-26256 CD denotes 32
T21133 26256-26257 NNP denotes ]
T21134 26257-26258 , denotes ,
T21135 26259-26262 CC denotes and
T21136 26263-26266 DT denotes the
T21137 26267-26276 VBN denotes activated
T21138 26277-26280 NN denotes p38
T21139 26281-26284 MD denotes can
T21140 26285-26294 VB denotes stimulate
T21141 26295-26300 NN denotes NF-κB
T21142 26301-26303 IN denotes by
T21143 26304-26305 DT denotes a
T21144 26306-26315 NN denotes mechanism
T21145 26316-26325 VBG denotes involving
T21146 26326-26333 NN denotes histone
T21147 26334-26343 NN denotes acetylase
T21148 26344-26361 JJ denotes p300/CREB-binding
T21149 26362-26369 NN denotes protein
T21150 26370-26371 NNP denotes [
T21151 26371-26373 CD denotes 33
T21152 26373-26374 NNP denotes ]
T21153 26374-26375 . denotes .
T21154 26376-26379 PRP$ denotes Our
T21155 26380-26387 NNS denotes results
T21156 26388-26396 VBD denotes revealed
T21157 26397-26401 IN denotes that
T21158 26402-26405 CD denotes p38
T21159 26406-26410 NNP denotes MAPK
T21160 26411-26421 NN denotes activation
T21161 26422-26425 VBD denotes was
T21162 26426-26429 RB denotes not
T21163 26430-26438 VBN denotes affected
T21164 26439-26441 IN denotes by
T21165 26442-26450 NNP denotes LY294002
T21166 26450-26451 , denotes ,
T21167 26452-26459 IN denotes whereas
T21168 26460-26465 NNP denotes NF-κB
T21169 26466-26473 JJ denotes binding
T21170 26474-26482 NN denotes activity
T21171 26483-26486 VBD denotes was
T21172 26487-26496 VBN denotes decreased
T21173 26497-26499 IN denotes by
T21174 26500-26508 NNP denotes LY294002
T21175 26508-26509 , denotes ,
T21176 26510-26515 WDT denotes which
T21177 26516-26524 VBD denotes provided
T21178 26525-26528 DT denotes the
T21179 26529-26537 NN denotes evidence
T21180 26538-26541 IN denotes for
T21181 26542-26543 DT denotes a
T21182 26544-26547 JJ denotes p38
T21183 26548-26552 NNP denotes MAPK
T21184 26553-26560 NN denotes pathway
T21185 26561-26572 JJ denotes independent
T21186 26573-26575 IN denotes of
T21187 26576-26580 CD denotes PI3K
T21188 26581-26591 NN denotes activation
T21189 26591-26592 . denotes .
T21190 26593-26596 DT denotes The
T21191 26597-26603 JJ denotes direct
T21192 26604-26616 NN denotes relationship
T21193 26617-26624 IN denotes between
T21194 26625-26628 CD denotes p38
T21195 26629-26632 CC denotes and
T21196 26633-26638 NNP denotes NF-κB
T21197 26639-26642 IN denotes for
T21198 26643-26648 NNP denotes IL-17
T21199 26649-26659 NN denotes production
T21200 26660-26665 VBZ denotes needs
T21201 26666-26668 TO denotes to
T21202 26669-26671 VB denotes be
T21203 26672-26679 VBN denotes studied
T21204 26680-26682 IN denotes in
T21205 26683-26689 JJ denotes future
T21206 26690-26701 NNS denotes experiments
T21207 26701-26702 . denotes .
T21208 26703-26706 DT denotes The
T21209 26707-26713 NN denotes search
T21210 26714-26717 IN denotes for
T21211 26718-26719 DT denotes a
T21212 26720-26730 JJ denotes downstream
T21213 26731-26738 NN denotes pathway
T21214 26739-26741 IN denotes of
T21215 26742-26746 NNP denotes PI3K
T21216 26747-26753 VBD denotes seemed
T21217 26754-26756 TO denotes to
T21218 26757-26761 VB denotes have
T21219 26762-26763 DT denotes a
T21220 26764-26771 JJ denotes maximal
T21221 26772-26780 NN denotes response
T21222 26781-26783 IN denotes of
T21223 26784-26787 NNP denotes Akt
T21224 26788-26798 NN denotes activation
T21225 26799-26801 IN denotes at
T21226 26802-26803 CD denotes 1
T21227 26804-26808 NN denotes hour
T21228 26809-26812 CC denotes and
T21229 26813-26814 DT denotes a
T21230 26815-26822 JJ denotes gradual
T21231 26823-26827 NN denotes loss
T21232 26828-26830 IN denotes of
T21233 26831-26839 NN denotes activity
T21234 26840-26842 IN denotes at
T21235 26843-26844 CD denotes 2
T21236 26845-26850 NNS denotes hours
T21237 26850-26851 . denotes .
T21238 26852-26855 DT denotes The
T21239 26856-26860 NN denotes fact
T21240 26861-26865 IN denotes that
T21241 26866-26869 NNP denotes Akt
T21242 26870-26872 VBZ denotes is
T21243 26873-26887 VBN denotes phosphorylated
T21244 26888-26892 IN denotes upon
T21245 26893-26901 JJ denotes anti-CD3
T21246 26902-26913 NN denotes stimulation
T21247 26914-26922 VBZ denotes suggests
T21248 26923-26926 DT denotes the
T21249 26927-26935 JJ denotes possible
T21250 26936-26947 NN denotes involvement
T21251 26948-26950 IN denotes of
T21252 26951-26955 NNP denotes PI3K
T21253 26956-26958 IN denotes in
T21254 26959-26962 DT denotes the
T21255 26963-26972 NN denotes induction
T21256 26973-26975 IN denotes of
T21257 26976-26981 NNP denotes IL-17
T21258 26982-26984 IN denotes in
T21259 26985-26987 NNP denotes RA
T21260 26987-26988 . denotes .
T21261 26989-26991 IN denotes In
T21262 26992-26996 NN denotes view
T21263 26997-26999 IN denotes of
T21264 27000-27003 DT denotes the
T21265 27004-27008 NN denotes fact
T21266 27009-27013 IN denotes that
T21267 27014-27019 NNP denotes NF-κB
T21268 27020-27023 VBD denotes was
T21269 27024-27028 RB denotes also
T21270 27029-27038 VBN denotes activated
T21271 27039-27041 IN denotes by
T21272 27042-27050 JJ denotes anti-CD3
T21273 27050-27051 NN denotes /
T21274 27051-27060 NN denotes anti-CD28
T21275 27060-27061 , denotes ,
T21276 27062-27067 NN denotes IL-15
T21277 27068-27070 CC denotes or
T21278 27071-27079 NNS denotes mitogens
T21279 27080-27082 IN denotes in
T21280 27083-27086 PRP$ denotes our
T21281 27087-27098 NNS denotes experiments
T21282 27098-27099 , denotes ,
T21283 27100-27102 PRP denotes it
T21284 27103-27105 VBZ denotes is
T21285 27106-27110 RBS denotes most
T21286 27111-27117 JJ denotes likely
T21287 27118-27122 IN denotes that
T21288 27123-27126 DT denotes the
T21289 27127-27132 NNP denotes NF-κB
T21290 27133-27140 NN denotes pathway
T21291 27141-27143 VBZ denotes is
T21292 27144-27148 RB denotes also
T21293 27149-27157 RB denotes actively
T21294 27158-27166 VBN denotes involved
T21295 27167-27169 IN denotes in
T21296 27170-27173 DT denotes the
T21297 27174-27183 NN denotes induction
T21298 27184-27186 IN denotes of
T21299 27187-27192 NNP denotes IL-17
T21300 27193-27195 IN denotes in
T21301 27196-27198 NNP denotes RA
T21302 27199-27203 NNP denotes PBMC
T21303 27203-27204 . denotes .
T21304 27205-27207 IN denotes In
T21305 27208-27216 NN denotes contrast
T21306 27216-27217 , denotes ,
T21307 27218-27221 DT denotes the
T21308 27222-27226 NNP denotes AP-1
T21309 27227-27233 NN denotes signal
T21310 27234-27246 NN denotes transduction
T21311 27247-27254 NN denotes pathway
T21312 27254-27255 , denotes ,
T21313 27256-27263 DT denotes another
T21314 27264-27273 JJ denotes important
T21315 27274-27283 VBG denotes signaling
T21316 27284-27291 NN denotes pathway
T21317 27292-27295 IN denotes for
T21318 27296-27316 NNS denotes cytokines/chemokines
T21319 27316-27317 , denotes ,
T21320 27318-27321 VBD denotes was
T21321 27322-27325 RB denotes not
T21322 27326-27335 VBN denotes activated
T21323 27336-27338 IN denotes in
T21324 27339-27342 PRP$ denotes our
T21325 27343-27354 NNS denotes experiments
T21326 27355-27356 -LRB- denotes (
T21327 27356-27360 NNS denotes data
T21328 27361-27364 RB denotes not
T21329 27365-27370 VBN denotes shown
T21330 27370-27371 -RRB- denotes )
T21331 27371-27372 . denotes .
T21332 27373-27381 IN denotes Although
T21333 27382-27386 NNP denotes PI3K
T21334 27387-27390 CC denotes and
T21335 27391-27394 PRP$ denotes its
T21336 27395-27405 JJ denotes downstream
T21337 27406-27412 NN denotes kinase
T21338 27413-27416 NNP denotes Akt
T21339 27417-27419 IN denotes in
T21340 27420-27431 NN denotes association
T21341 27432-27436 IN denotes with
T21342 27437-27442 NNP denotes NF-κB
T21343 27443-27447 VBP denotes have
T21344 27448-27452 VBN denotes been
T21345 27453-27461 VBN denotes reported
T21346 27462-27464 TO denotes to
T21347 27465-27472 VB denotes deliver
T21348 27473-27483 VBG denotes activating
T21349 27484-27491 NNS denotes signals
T21350 27492-27494 IN denotes in
T21351 27495-27499 JJ denotes many
T21352 27500-27504 NN denotes cell
T21353 27505-27510 NNS denotes types
T21354 27510-27511 , denotes ,
T21355 27512-27515 DT denotes the
T21356 27516-27520 NNS denotes data
T21357 27521-27523 IN denotes on
T21358 27524-27527 DT denotes the
T21359 27528-27534 NN denotes signal
T21360 27535-27543 VBG denotes inducing
T21361 27544-27549 NNP denotes IL-17
T21362 27550-27553 VBP denotes are
T21363 27554-27561 VBG denotes lacking
T21364 27561-27562 . denotes .
T21365 27563-27566 PRP$ denotes Our
T21366 27567-27571 NNS denotes data
T21367 27572-27579 RB denotes clearly
T21368 27580-27592 VBD denotes demonstrated
T21369 27593-27597 IN denotes that
T21370 27598-27606 NNP denotes PI3K/Akt
T21371 27607-27610 CC denotes and
T21372 27611-27620 JJ denotes resultant
T21373 27621-27626 NN denotes NF-κB
T21374 27627-27637 NN denotes activation
T21375 27638-27643 MD denotes could
T21376 27644-27646 VB denotes be
T21377 27647-27649 DT denotes an
T21378 27650-27659 JJ denotes important
T21379 27660-27670 NN denotes arbitrator
T21380 27671-27673 IN denotes of
T21381 27674-27677 DT denotes the
T21382 27678-27690 NN denotes upregulation
T21383 27691-27693 IN denotes of
T21384 27694-27699 NNP denotes IL-17
T21385 27700-27702 IN denotes in
T21386 27703-27705 NNP denotes RA
T21387 27705-27706 , denotes ,
T21388 27707-27709 IN denotes on
T21389 27710-27713 DT denotes the
T21390 27714-27719 NN denotes basis
T21391 27720-27722 IN denotes of
T21392 27723-27726 PRP$ denotes our
T21393 27727-27738 NNS denotes experiments
T21394 27739-27746 VBG denotes showing
T21395 27747-27759 JJ denotes simultaneous
T21396 27760-27768 VBG denotes blocking
T21397 27769-27771 IN denotes of
T21398 27772-27777 JJ denotes NF-κB
T21399 27778-27785 JJ denotes binding
T21400 27786-27794 NN denotes activity
T21401 27795-27797 IN denotes in
T21402 27798-27801 DT denotes the
T21403 27802-27807 NNP denotes IL-17
T21404 27808-27816 NN denotes promoter
T21405 27817-27819 IN denotes by
T21406 27820-27824 NNP denotes PDTC
T21407 27825-27828 CC denotes and
T21408 27829-27837 NNP denotes LY294002
T21409 27837-27838 . denotes .
T21410 27839-27850 VBG denotes Considering
T21411 27851-27854 PRP$ denotes its
T21412 27855-27870 JJ denotes proinflammatory
T21413 27871-27881 NNS denotes activities
T21414 27882-27885 CC denotes and
T21415 27886-27896 JJ denotes successful
T21416 27897-27906 NN denotes induction
T21417 27907-27909 IN denotes of
T21418 27910-27920 JJ denotes anti-IL-17
T21419 27921-27924 IN denotes for
T21420 27925-27937 VBG denotes ameliorating
T21421 27938-27947 NNS denotes arthritis
T21422 27948-27950 IN denotes in
T21423 27951-27957 NN denotes animal
T21424 27958-27964 NNS denotes models
T21425 27965-27966 NNP denotes [
T21426 27966-27975 CD denotes 2,6,34-36
T21427 27975-27976 NNP denotes ]
T21428 27976-27977 , denotes ,
T21429 27978-27991 VBG denotes understanding
T21430 27992-27995 DT denotes the
T21431 27996-28001 NNP denotes IL-17
T21432 28002-28011 VBG denotes signaling
T21433 28012-28019 NN denotes pathway
T21434 28020-28022 VBZ denotes is
T21435 28023-28025 DT denotes an
T21436 28026-28035 JJ denotes important
T21437 28036-28043 NN denotes element
T21438 28044-28046 IN denotes of
T21439 28047-28057 VBG denotes developing
T21440 28058-28061 JJ denotes new
T21441 28062-28070 JJ denotes targeted
T21442 28071-28080 NNS denotes therapies
T21443 28081-28083 IN denotes in
T21444 28084-28086 NNP denotes RA
T21445 28086-28087 . denotes .
T22443 28101-28103 PRP denotes We
T22444 28104-28108 VBP denotes have
T22445 28109-28117 VBN denotes detected
T22446 28118-28119 DT denotes a
T22447 28120-28124 RBR denotes more
T22448 28125-28135 JJ denotes pronounced
T22449 28136-28146 NN denotes production
T22450 28147-28149 IN denotes of
T22451 28150-28155 NNP denotes IL-17
T22452 28156-28160 IN denotes from
T22453 28161-28163 NNP denotes RA
T22454 28164-28168 NNP denotes PBMC
T22455 28169-28171 IN denotes in
T22456 28172-28180 NN denotes response
T22457 28181-28183 TO denotes to
T22458 28184-28189 NNP denotes IL-15
T22459 28190-28193 CC denotes and
T22460 28194-28199 NNP denotes MCP-1
T22461 28200-28202 RB denotes as
T22462 28203-28207 RB denotes well
T22463 28208-28210 IN denotes as
T22464 28211-28222 NN denotes stimulation
T22465 28223-28225 IN denotes by
T22466 28226-28234 JJ denotes anti-CD3
T22467 28234-28235 NN denotes /
T22468 28235-28244 NN denotes anti-CD28
T22469 28244-28245 . denotes .
T22470 28246-28248 PRP denotes We
T22471 28249-28253 VBP denotes have
T22472 28254-28258 RB denotes also
T22473 28259-28264 VBN denotes shown
T22474 28265-28269 IN denotes that
T22475 28270-28282 NN denotes upregulation
T22476 28283-28285 IN denotes of
T22477 28286-28291 NNP denotes IL-17
T22478 28292-28294 IN denotes by
T22479 28295-28304 VBN denotes activated
T22480 28305-28306 NNP denotes T
T22481 28307-28312 NNS denotes cells
T22482 28313-28315 IN denotes in
T22483 28316-28324 NNS denotes patients
T22484 28325-28329 IN denotes with
T22485 28330-28332 NNP denotes RA
T22486 28333-28338 MD denotes could
T22487 28339-28341 VB denotes be
T22488 28342-28345 DT denotes the
T22489 28346-28352 NN denotes result
T22490 28353-28355 IN denotes of
T22491 28356-28366 NN denotes activation
T22492 28367-28370 IN denotes via
T22493 28371-28374 DT denotes the
T22494 28375-28383 JJ denotes PI3K/Akt
T22495 28384-28391 NN denotes pathway
T22496 28392-28396 IN denotes with
T22497 28397-28406 JJ denotes resultant
T22498 28407-28412 NN denotes NF-κB
T22499 28413-28423 NN denotes activation
T22500 28423-28424 . denotes .
T22501 28425-28428 PRP$ denotes Our
T22502 28429-28433 NNS denotes data
T22503 28434-28441 VBP denotes provide
T22504 28442-28450 NNS denotes insights
T22505 28451-28455 IN denotes into
T22506 28456-28464 JJ denotes cellular
T22507 28465-28475 NNS denotes mechanisms
T22508 28476-28478 IN denotes of
T22509 28479-28482 DT denotes the
T22510 28483-28493 NN denotes regulation
T22511 28494-28496 IN denotes of
T22512 28497-28502 JJ denotes IL-17
T22513 28503-28513 NN denotes production
T22514 28514-28516 IN denotes in
T22515 28517-28519 NNP denotes RA
T22516 28519-28520 , denotes ,
T22517 28521-28524 CC denotes and
T22518 28525-28534 VB denotes highlight
T22519 28535-28538 DT denotes the
T22520 28539-28543 NN denotes role
T22521 28544-28546 IN denotes of
T22522 28547-28548 NNP denotes T
T22523 28549-28554 NNS denotes cells
T22524 28554-28555 , denotes ,
T22525 28556-28561 WDT denotes which
T22526 28562-28565 VBZ denotes has
T22527 28566-28574 RB denotes hitherto
T22528 28575-28579 VBN denotes been
T22529 28580-28589 VBN denotes neglected
T22530 28590-28592 IN denotes in
T22531 28593-28595 NNP denotes RA
T22532 28596-28608 NN denotes pathogenesis
T22533 28608-28609 . denotes .
T22534 28610-28618 RB denotes Together
T22535 28619-28623 IN denotes with
T22536 28624-28630 JJ denotes recent
T22537 28631-28635 NNS denotes data
T22538 28636-28638 IN denotes on
T22539 28639-28642 DT denotes the
T22540 28643-28653 JJ denotes successful
T22541 28654-28666 NN denotes introduction
T22542 28667-28669 IN denotes of
T22543 28670-28680 NN denotes anti-IL-17
T22544 28681-28683 IN denotes in
T22545 28684-28686 NNP denotes RA
T22546 28686-28687 , denotes ,
T22547 28688-28691 PRP$ denotes our
T22548 28692-28699 NNS denotes results
T22549 28700-28704 VBP denotes have
T22550 28705-28710 VBN denotes added
T22551 28711-28722 NN denotes information
T22552 28723-28726 IN denotes for
T22553 28727-28730 DT denotes the
T22554 28731-28737 JJ denotes future
T22555 28738-28747 JJ denotes molecular
T22556 28748-28757 VBG denotes targeting
T22557 28758-28760 IN denotes of
T22558 28761-28764 JJ denotes new
T22559 28765-28776 JJ denotes therapeutic
T22560 28777-28789 NNS denotes applications
T22561 28790-28792 IN denotes in
T22562 28793-28795 NNP denotes RA
T22563 28795-28796 . denotes .
R1064 T1506 T1505 prep for,Evidence
R1065 T1507 T1508 det the,expression
R1066 T1508 T1506 pobj expression,for
R10760 T20377 T20380 nsubjpass IL-17,described
R10761 T20378 T20380 auxpass was,described
R10762 T20379 T20380 advmod first,described
R10763 T20380 T20380 ROOT described,described
R10764 T20381 T20380 prep as,described
R10765 T20382 T20385 det a,product
R10766 T20383 T20385 compound T,product
R10767 T20384 T20385 compound cell,product
R10768 T20385 T20381 pobj product,as
R10769 T20386 T20385 prep with,product
R10770 T20387 T20388 amod proinflammatory,properties
R10771 T20388 T20386 pobj properties,with
R10772 T20389 T20391 compound [,]
R10773 T20390 T20391 compound "5,22",]
R10774 T20391 T20388 appos ],properties
R10775 T20392 T20380 punct .,described
R10776 T20393 T20395 nsubjpass RA,characterized
R10777 T20394 T20395 auxpass is,characterized
R10778 T20395 T20395 ROOT characterized,characterized
R10779 T20396 T20395 agent by,characterized
R10780 T20397 T20396 pobj hyperplasia,by
R10781 T20398 T20397 prep of,hyperplasia
R10782 T20399 T20401 amod synovial,cells
R10783 T20400 T20401 compound lining,cells
R10784 T20401 T20398 pobj cells,of
R10785 T20402 T20397 cc and,hyperplasia
R10786 T20403 T20405 det an,infiltration
R10787 T20404 T20405 amod intense,infiltration
R10788 T20405 T20397 conj infiltration,hyperplasia
R10789 T20406 T20405 prep by,infiltration
R10790 T20407 T20408 compound mononuclear,cells
R10791 T20408 T20406 pobj cells,by
R10792 T20409 T20411 nmod [,]
R10793 T20410 T20411 nummod 23,]
R10794 T20411 T20408 appos ],cells
R10795 T20412 T20395 punct .,characterized
R10796 T20413 T20414 amod Proinflammatory,cytokines
R10797 T20414 T20420 nsubj cytokines,are
R10798 T20415 T20416 amod such,as
R10799 T20416 T20414 prep as,cytokines
R10800 T20417 T20416 pobj IL-1,as
R10801 T20418 T20417 cc and,IL-1
R10802 T20419 T20417 conj TNF-α,IL-1
R10803 T20420 T20420 ROOT are,are
R10804 T20421 T20420 acomp abundant,are
R10805 T20422 T20421 prep in,abundant
R10806 T20423 T20424 amod rheumatoid,synovium
R10807 T20424 T20422 pobj synovium,in
R10808 T20425 T20420 punct ",",are
R10809 T20426 T20439 mark whereas,proved
R10810 T20427 T20430 det the,cytokines
R10811 T20428 T20430 nmod T,cytokines
R10812 T20429 T20430 amod cell-derived,cytokines
R10813 T20430 T20439 nsubj cytokines,proved
R10814 T20431 T20430 punct ",",cytokines
R10815 T20432 T20433 advmod especially,IL-4
R10816 T20433 T20430 appos IL-4,cytokines
R10817 T20434 T20433 cc and,IL-4
R10818 T20435 T20433 conj interferon-γ,IL-4
R10819 T20436 T20439 punct ",",proved
R10820 T20437 T20439 aux have,proved
R10821 T20438 T20439 advmod often,proved
R10822 T20439 T20420 advcl proved,are
R10823 T20440 T20439 oprd difficult,proved
R10824 T20441 T20442 aux to,detect
R10825 T20442 T20440 xcomp detect,difficult
R10826 T20443 T20442 prep in,detect
R10827 T20444 T20445 compound RA,synovium
R10828 T20445 T20446 compound synovium,[
R10829 T20446 T20443 pobj [,in
R10830 T20447 T20448 nummod 24,]
R10831 T20448 T20446 appos ],[
R10832 T20449 T20420 punct .,are
R10833 T20450 T20454 mark Although,have
R10834 T20451 T20452 compound T,cells
R10835 T20452 T20454 nsubj cells,have
R10836 T20453 T20454 aux may,have
R10837 T20454 T20472 advcl have,limited
R10838 T20455 T20456 det a,role
R10839 T20456 T20454 dobj role,have
R10840 T20457 T20456 prep in,role
R10841 T20458 T20459 det the,augmentation
R10842 T20459 T20457 pobj augmentation,in
R10843 T20460 T20459 prep of,augmentation
R10844 T20461 T20463 amod rheumatoid,inflammation
R10845 T20462 T20463 amod synovial,inflammation
R10846 T20463 T20460 pobj inflammation,of
R10847 T20464 T20459 punct ",",augmentation
R10848 T20465 T20466 det the,lack
R10849 T20466 T20472 nsubj lack,limited
R10850 T20467 T20466 prep of,lack
R10851 T20468 T20470 nmod T,cytokines
R10852 T20469 T20470 amod cell-derived,cytokines
R10853 T20470 T20467 pobj cytokines,of
R10854 T20471 T20472 aux has,limited
R10855 T20472 T20472 ROOT limited,limited
R10856 T20473 T20474 poss its,importance
R10857 T20474 T20472 dobj importance,limited
R10858 T20475 T20472 punct .,limited
R10859 T20476 T20482 prep In,appealing
R10860 T20477 T20478 det this,respect
R10861 T20478 T20476 pobj respect,In
R10862 T20479 T20482 punct ",",appealing
R10863 T20480 T20482 nsubj IL-17,appealing
R10864 T20481 T20482 aux is,appealing
R10865 T20482 T20482 ROOT appealing,appealing
R10866 T20483 T20487 mark because,described
R10867 T20484 T20487 nsubjpass it,described
R10868 T20485 T20487 aux has,described
R10869 T20486 T20487 auxpass been,described
R10870 T20487 T20482 advcl described,appealing
R10871 T20488 T20487 prep as,described
R10872 T20489 T20492 det a,cytokine
R10873 T20490 T20492 nmod T,cytokine
R10874 T20491 T20492 amod cell-derived,cytokine
R10875 T20492 T20488 pobj cytokine,as
R10876 T20493 T20492 prep with,cytokine
R10877 T20494 T20495 amod proinflammatory,properties
R10878 T20495 T20493 pobj properties,with
R10879 T20496 T20482 punct .,appealing
R10880 T20497 T20502 prep In,tried
R10881 T20498 T20499 poss our,studies
R10882 T20499 T20497 pobj studies,In
R10883 T20500 T20502 punct ",",tried
R10884 T20501 T20502 nsubj we,tried
R10885 T20502 T20502 ROOT tried,tried
R10886 T20503 T20504 aux to,evaluate
R10887 T20504 T20502 xcomp evaluate,tried
R10888 T20505 T20509 advmod how,regulated
R10889 T20506 T20507 amod IL-17,production
R10890 T20507 T20509 nsubjpass production,regulated
R10891 T20508 T20509 auxpass is,regulated
R10892 T20509 T20504 ccomp regulated,evaluate
R10893 T20510 T20509 prep in,regulated
R10894 T20511 T20512 compound RA,PBMC
R10895 T20512 T20510 pobj PBMC,in
R10896 T20513 T20509 punct ",",regulated
R10897 T20514 T20509 cc and,regulated
R10898 T20515 T20516 det which,signaling
R10899 T20516 T20502 advcl signaling,tried
R10900 T20517 T20516 dobj pathway,signaling
R10901 T20518 T20519 nsubj it,used
R10902 T20519 T20517 relcl used,pathway
R10903 T20520 T20502 punct .,tried
R10904 T20521 T20525 nsubjpass Levels,found
R10905 T20522 T20521 prep of,Levels
R10906 T20523 T20522 pobj IL-17,of
R10907 T20524 T20525 auxpass were,found
R10908 T20525 T20525 ROOT found,found
R10909 T20526 T20527 aux to,be
R10910 T20527 T20525 xcomp be,found
R10911 T20528 T20527 acomp higher,be
R10912 T20529 T20527 prep in,be
R10913 T20530 T20532 nmod RA,fluid
R10914 T20531 T20532 compound synovial,fluid
R10915 T20532 T20529 pobj fluid,in
R10916 T20558 T20545 punct ",",are
R10917 T20533 T20525 prep than,found
R10918 T20559 T20568 mark although,achieved
R10919 T20534 T20533 prep in,than
R10920 T20560 T20562 det the,level
R10921 T20561 T20562 amod highest,level
R10922 T20562 T20568 nsubjpass level,achieved
R10923 T20563 T20562 prep of,level
R10924 T20564 T20565 compound IL-17,production
R10925 T20565 T20563 pobj production,of
R10926 T20535 T20534 pobj OA,in
R10927 T20566 T20568 aux can,achieved
R10928 T20567 T20568 auxpass be,achieved
R10929 T20568 T20545 advcl achieved,are
R10930 T20536 T20537 compound synovial,fluid
R10931 T20569 T20568 agent by,achieved
R10932 T20570 T20573 amod anti-CD3,stimulation
R10933 T20571 T20573 nmod /,stimulation
R10934 T20572 T20573 amod anti-CD28,stimulation
R10935 T20537 T20540 det fluid,]
R10936 T20573 T20569 pobj stimulation,by
R10937 T20574 T20568 prep in,achieved
R10938 T20575 T20576 amod healthy,individuals
R10939 T20538 T20540 nmod [,]
R10940 T20576 T20574 pobj individuals,in
R10941 T20577 T20579 nmod [,]
R10942 T20578 T20579 nummod 25,]
R10943 T20539 T20540 nummod 15,]
R10944 T20579 T20576 appos ],individuals
R10945 T20580 T20545 punct .,are
R10946 T20581 T20603 prep In,increased
R10947 T20540 T20540 ROOT ],]
R10948 T20582 T20583 poss our,experiments
R10949 T20583 T20581 pobj experiments,In
R10950 T20541 T20525 punct .,found
R10951 T20584 T20603 punct ",",increased
R10952 T20585 T20603 nsubj PHA,increased
R10953 T20586 T20585 prep as,PHA
R10954 T20542 T20545 advmod However,are
R10955 T20587 T20586 pobj mitogens,as
R10956 T20588 T20587 punct ",",mitogens
R10957 T20589 T20591 advmod as,as
R10958 T20543 T20545 punct ",",are
R10959 T20590 T20591 advmod well,as
R10960 T20591 T20587 cc as,mitogens
R10961 T20592 T20594 amod anti-CD3,anti-CD28
R10962 T20544 T20545 expl there,are
R10963 T20593 T20594 compound /,anti-CD28
R10964 T20594 T20587 conj anti-CD28,mitogens
R10965 T20595 T20587 prep for,mitogens
R10966 T20545 T20545 ROOT are,are
R10967 T20596 T20595 pcomp signaling,for
R10968 T20597 T20596 prep through,signaling
R10969 T20546 T20547 amod few,data
R10970 T20598 T20601 det the,receptor
R10971 T20599 T20601 compound T,receptor
R10972 T20600 T20601 compound cell,receptor
R10973 T20601 T20597 pobj receptor,through
R10974 T20602 T20603 punct ",",increased
R10975 T20603 T20603 ROOT increased,increased
R10976 T20604 T20605 compound IL-17,production
R10977 T20605 T20603 dobj production,increased
R10978 T20547 T20545 attr data,are
R10979 T20606 T20605 prep from,production
R10980 T20607 T20608 compound RA,PBMC
R10981 T20608 T20606 pobj PBMC,from
R10982 T20548 T20547 amod available,data
R10983 T20609 T20603 prep in,increased
R10984 T20610 T20612 det a,manner
R10985 T20611 T20612 amod dose-dependent,manner
R10986 T20549 T20547 prep on,data
R10987 T20612 T20609 pobj manner,in
R10988 T20550 T20551 det the,agents
R10989 T20613 T20603 punct .,increased
R10990 T20551 T20549 pobj agents,on
R10991 T20614 T20615 nsubj We,found
R10992 T20615 T20615 ROOT found,found
R10993 T20552 T20553 nsubj that,stimulate
R10994 T20616 T20615 punct ",",found
R10995 T20617 T20615 prep by,found
R10996 T20618 T20621 det a,assay
R10997 T20553 T20551 relcl stimulate,agents
R10998 T20619 T20620 compound cell,proliferation
R10999 T20620 T20621 compound proliferation,assay
R11000 T20554 T20555 amod IL-17,production
R11001 T20621 T20617 pobj assay,by
R11002 T20622 T20623 punct (,data
R11003 T20555 T20553 dobj production,stimulate
R11004 T20623 T20615 advcl data,found
R11005 T20624 T20625 neg not,shown
R11006 T20556 T20555 prep in,production
R11007 T20625 T20623 acl shown,data
R11008 T20626 T20623 punct ),data
R11009 T20557 T20556 pobj RA,in
R11010 T20627 T20634 punct ",",be
R11011 T20628 T20634 mark that,be
R11012 T20629 T20630 det this,upregulation
R11013 T20655 T20650 pobj cells,by
R11014 T20630 T20634 nsubj upregulation,be
R11015 T20631 T20630 prep of,upregulation
R11016 T20632 T20631 pobj IL-17,of
R11017 T20633 T20634 aux might,be
R11018 T20656 T20655 punct ",",cells
R11019 T20634 T20623 ccomp be,data
R11020 T20635 T20634 acomp due,be
R11021 T20636 T20635 pcomp to,due
R11022 T20637 T20639 amod increased,activity
R11023 T20638 T20639 amod cellular,activity
R11024 T20639 T20636 pobj activity,to
R11025 T20657 T20658 advmod especially,for
R11026 T20640 T20641 advmod rather,than
R11027 T20641 T20634 cc than,be
R11028 T20642 T20641 prep to,than
R11029 T20643 T20644 amod cellular,proliferation
R11030 T20658 T20650 prep for,by
R11031 T20644 T20642 pobj proliferation,to
R11032 T20645 T20615 punct .,found
R11033 T20646 T20648 nsubjpass IL-17,produced
R11034 T20659 T20660 amod Th1/Th0,cells
R11035 T20647 T20648 auxpass is,produced
R11036 T20648 T20648 ROOT produced,produced
R11037 T20649 T20650 advmod mainly,by
R11038 T20660 T20658 pobj cells,for
R11039 T20650 T20648 agent by,produced
R11040 T20651 T20655 amod activated,cells
R11041 T20661 T20658 punct ",",for
R11042 T20652 T20655 compound CD4,cells
R11043 T20653 T20655 nummod +,cells
R11044 T20654 T20655 compound T,cells
R11045 T20662 T20650 neg not,by
R11046 T20663 T20665 det the,phenotype
R11047 T20664 T20665 amod Th2,phenotype
R11048 T20665 T20648 npadvmod phenotype,produced
R11049 T20752 T20753 compound rheumatoid,synoviocytes
R11050 T20666 T20665 appos [,phenotype
R11051 T20753 T20751 dobj synoviocytes,stimulates
R11052 T20754 T20755 aux to,secrete
R11053 T20667 T20668 nummod 26,]
R11054 T20755 T20751 xcomp secrete,stimulates
R11055 T20756 T20757 amod several,cytokines
R11056 T20757 T20755 dobj cytokines,secrete
R11057 T20668 T20665 conj ],phenotype
R11058 T20758 T20759 amod such,as
R11059 T20759 T20757 prep as,cytokines
R11060 T20669 T20648 punct .,produced
R11061 T20760 T20759 pobj IL-6,as
R11062 T20761 T20760 punct ",",IL-6
R11063 T20762 T20760 conj IL-8,IL-6
R11064 T20670 T20676 advmod However,produced
R11065 T20763 T20762 cc and,IL-8
R11066 T20764 T20765 compound tumor,necrosis
R11067 T20671 T20676 punct ",",produced
R11068 T20765 T20767 compound necrosis,gene
R11069 T20766 T20767 compound factor-stimulated,gene
R11070 T20767 T20755 npadvmod gene,secrete
R11071 T20768 T20767 nummod 6,gene
R11072 T20672 T20676 nsubjpass it,produced
R11073 T20769 T20771 advmod as,as
R11074 T20673 T20676 aux can,produced
R11075 T20770 T20771 advmod well,as
R11076 T20771 T20767 cc as,gene
R11077 T20772 T20773 compound prostaglandin,E2
R11078 T20674 T20676 advmod also,produced
R11079 T20773 T20767 conj E2,gene
R11080 T20774 T20755 prep in,secrete
R11081 T20775 T20778 amod vitro,]
R11082 T20675 T20676 auxpass be,produced
R11083 T20776 T20778 compound [,]
R11084 T20777 T20778 compound "12,28,29",]
R11085 T20676 T20676 ROOT produced,produced
R11086 T20778 T20774 pobj ],in
R11087 T20779 T20751 punct .,stimulates
R11088 T20677 T20676 agent by,produced
R11089 T20780 T20781 expl There,are
R11090 T20781 T20781 ROOT are,are
R11091 T20782 T20784 advmod as,few
R11092 T20678 T20681 nummod CD8,cells
R11093 T20783 T20784 advmod yet,few
R11094 T20784 T20785 nummod few,data
R11095 T20679 T20681 nummod +,cells
R11096 T20785 T20781 attr data,are
R11097 T20786 T20785 amod available,data
R11098 T20787 T20786 prep on,available
R11099 T20680 T20681 compound T,cells
R11100 T20788 T20789 det the,agents
R11101 T20789 T20787 pobj agents,on
R11102 T20790 T20791 nsubj that,stimulate
R11103 T20681 T20677 pobj cells,by
R11104 T20791 T20789 relcl stimulate,agents
R11105 T20792 T20793 amod IL-17,production
R11106 T20793 T20791 dobj production,stimulate
R11107 T20682 T20676 prep via,produced
R11108 T20794 T20793 prep in,production
R11109 T20795 T20794 pobj RA,in
R11110 T20683 T20686 det an,mechanism
R11111 T20796 T20781 punct ",",are
R11112 T20797 T20807 mark although,known
R11113 T20684 T20686 amod IL-23,mechanism
R11114 T20798 T20799 det some,cytokines
R11115 T20799 T20807 nsubjpass cytokines,known
R11116 T20685 T20686 amod triggering,mechanism
R11117 T20800 T20801 punct (,IL-15
R11118 T20801 T20799 appos IL-15,cytokines
R11119 T20802 T20801 cc and,IL-15
R11120 T20686 T20682 pobj mechanism,via
R11121 T20803 T20801 conj IL-23,IL-15
R11122 T20804 T20799 punct ),cytokines
R11123 T20805 T20807 aux have,known
R11124 T20806 T20807 auxpass been,known
R11125 T20807 T20781 advcl known,are
R11126 T20808 T20809 aux to,regulate
R11127 T20809 T20807 xcomp regulate,known
R11128 T20810 T20811 compound IL-17,production
R11129 T20687 T20686 prep in,mechanism
R11130 T20811 T20809 dobj production,regulate
R11131 T20812 T20814 nmod [,]
R11132 T20813 T20814 nummod "13,14",]
R11133 T20688 T20690 amod Gram-negative,infection
R11134 T20814 T20781 attr ],are
R11135 T20815 T20781 punct .,are
R11136 T20816 T20818 nsubj We,investigated
R11137 T20689 T20690 amod pulmonary,infection
R11138 T20817 T20818 advmod therefore,investigated
R11139 T20818 T20818 ROOT investigated,investigated
R11140 T20819 T20828 det the,responding
R11141 T20690 T20687 pobj infection,in
R11142 T20820 T20828 prep in,responding
R11143 T20821 T20822 amod vitro,production
R11144 T20822 T20820 pobj production,in
R11145 T20691 T20693 nmod [,]
R11146 T20823 T20822 prep of,production
R11147 T20824 T20823 pobj IL-17,of
R11148 T20825 T20822 prep in,production
R11149 T20692 T20693 nummod 14,]
R11150 T20826 T20827 compound RA,PBMC
R11151 T20827 T20825 pobj PBMC,in
R11152 T20828 T20818 dobj responding,investigated
R11153 T20693 T20686 appos ],mechanism
R11154 T20829 T20828 prep to,responding
R11155 T20830 T20831 det a,variety
R11156 T20831 T20829 pobj variety,to
R11157 T20694 T20676 punct .,produced
R11158 T20832 T20831 prep of,variety
R11159 T20833 T20832 pobj cytokines/chemokines,of
R11160 T20834 T20833 cc and,cytokines/chemokines
R11161 T20695 T20702 prep In,augmented
R11162 T20835 T20833 conj mitogens,cytokines/chemokines
R11163 T20836 T20838 advmod as,as
R11164 T20696 T20695 pobj addition,In
R11165 T20837 T20838 advmod well,as
R11166 T20838 T20833 cc as,cytokines/chemokines
R11167 T20839 T20841 compound T,receptor
R11168 T20697 T20702 punct ",",augmented
R11169 T20840 T20841 compound cell,receptor
R11170 T20841 T20845 nsubj receptor,ligation
R11171 T20842 T20843 punct (,TCR
R11172 T20843 T20841 appos TCR,receptor
R11173 T20844 T20841 punct ),receptor
R11174 T20845 T20818 ccomp ligation,investigated
R11175 T20846 T20818 advcl using,investigated
R11176 T20698 T20699 compound IL-17,production
R11177 T20847 T20849 amod anti-CD3,anti-CD28
R11178 T20699 T20702 nsubjpass production,augmented
R11179 T20848 T20849 compound /,anti-CD28
R11180 T20700 T20702 auxpass was,augmented
R11181 T20701 T20702 advmod significantly,augmented
R11182 T20702 T20702 ROOT augmented,augmented
R11183 T20849 T20846 dobj anti-CD28,using
R11184 T20703 T20702 agent by,augmented
R11185 T20850 T20818 punct .,investigated
R11186 T20851 T20852 poss Our,studies
R11187 T20852 T20853 nsubj studies,demonstrated
R11188 T20704 T20705 compound T,cells
R11189 T20853 T20853 ROOT demonstrated,demonstrated
R11190 T20854 T20864 mark that,increased
R11191 T20855 T20862 nsubj IL-15,ligation
R11192 T20705 T20703 pobj cells,by
R11193 T20856 T20855 cc and,IL-15
R11194 T20857 T20855 conj MCP-1,IL-15
R11195 T20706 T20705 acl recognizing,cells
R11196 T20858 T20860 advmod as,as
R11197 T20859 T20860 advmod well,as
R11198 T20860 T20857 cc as,MCP-1
R11199 T20707 T20709 compound type,collagen
R11200 T20861 T20862 compound TCR,ligation
R11201 T20862 T20864 nsubj ligation,increased
R11202 T20863 T20864 advmod significantly,increased
R11203 T20708 T20709 compound II,collagen
R11204 T20864 T20853 ccomp increased,demonstrated
R11205 T20865 T20866 det the,production
R11206 T20866 T20864 dobj production,increased
R11207 T20709 T20706 dobj collagen,recognizing
R11208 T20867 T20866 prep of,production
R11209 T20868 T20867 pobj IL-17,of
R11210 T20869 T20864 prep in,increased
R11211 T20710 T20706 prep in,recognizing
R11212 T20870 T20871 compound RA,PBMC
R11213 T20871 T20869 pobj PBMC,in
R11214 T20872 T20853 punct .,demonstrated
R11215 T20711 T20714 det a,model
R11216 T20873 T20873 ROOT Adding,Adding
R11217 T20874 T20873 dobj IL-15,Adding
R11218 T20875 T20874 cc or,IL-15
R11219 T20712 T20714 amod collagen-induced,model
R11220 T20876 T20874 conj MCP-1,IL-15
R11221 T20877 T20873 prep to,Adding
R11222 T20878 T20879 compound TCR,ligation
R11223 T20879 T20880 dep ligation,augmented
R11224 T20880 T20873 nsubj augmented,Adding
R11225 T20881 T20882 amod IL-17,production
R11226 T20713 T20714 compound arthritis,model
R11227 T20882 T20880 dobj production,augmented
R11228 T20883 T20884 advmod more,markedly
R11229 T20884 T20880 advmod markedly,augmented
R11230 T20885 T20880 punct .,augmented
R11231 T20714 T20710 pobj model,in
R11232 T20886 T20910 prep In,affect
R11233 T20887 T20886 pobj contrast,In
R11234 T20888 T20910 punct ",",affect
R11235 T20715 T20717 nmod [,]
R11236 T20889 T20910 nsubj IL-1,affect
R11237 T20890 T20889 cc and,IL-1
R11238 T20891 T20889 conj TNF-α,IL-1
R11239 T20716 T20717 nummod 27,]
R11240 T20892 T20889 punct ",",IL-1
R11241 T20893 T20895 nsubjpass which,known
R11242 T20894 T20895 auxpass are,known
R11243 T20717 T20714 appos ],model
R11244 T20895 T20889 relcl known,IL-1
R11245 T20896 T20897 aux to,have
R11246 T20897 T20895 xcomp have,known
R11247 T20718 T20702 punct .,augmented
R11248 T20898 T20899 amod proinflammatory,properties
R11249 T20899 T20897 dobj properties,have
R11250 T20900 T20897 cc and,have
R11251 T20719 T20721 det A,interaction
R11252 T20901 T20903 aux to,increased
R11253 T20902 T20903 auxpass be,increased
R11254 T20903 T20895 xcomp increased,known
R11255 T20904 T20903 prep in,increased
R11256 T20720 T20721 amod complex,interaction
R11257 T20905 T20906 compound RA,joints
R11258 T20906 T20904 pobj joints,in
R11259 T20907 T20889 punct ",",IL-1
R11260 T20721 T20729 nsubj interaction,produce
R11261 T20908 T20910 aux did,affect
R11262 T20909 T20910 neg not,affect
R11263 T20722 T20721 prep between,interaction
R11264 T20910 T20910 ROOT affect,affect
R11265 T20911 T20912 amod IL-17,production
R11266 T20723 T20722 pobj cells,between
R11267 T20912 T20910 dobj production,affect
R11268 T20724 T20723 prep in,cells
R11269 T20913 T20910 punct .,affect
R11270 T20914 T20915 poss Our,data
R11271 T20725 T20727 amod inflamed,joints
R11272 T20915 T20916 nsubj data,were
R11273 T20916 T20916 ROOT were,were
R11274 T20917 T20916 acomp consistent,were
R11275 T20918 T20917 prep with,consistent
R11276 T20919 T20921 det a,report
R11277 T20920 T20921 amod recent,report
R11278 T20726 T20727 compound RA,joints
R11279 T20921 T20918 pobj report,with
R11280 T20922 T20924 dobj that,triggered
R11281 T20923 T20924 nsubj IL-15,triggered
R11282 T20727 T20724 pobj joints,in
R11283 T20924 T20921 acl triggered,report
R11284 T20925 T20924 prep in,triggered
R11285 T20926 T20928 amod vitro,production
R11286 T20728 T20729 aux might,produce
R11287 T20729 T20729 ROOT produce,produce
R11288 T20927 T20928 compound IL-17,production
R11289 T20928 T20925 pobj production,in
R11290 T20730 T20731 det a,variety
R11291 T20929 T20928 prep in,production
R11292 T20930 T20929 pobj PBMC,in
R11293 T20931 T20916 punct ",",were
R11294 T20731 T20729 dobj variety,produce
R11295 T20932 T20916 cc but,were
R11296 T20933 T20936 nsubj TNF-α,do
R11297 T20934 T20936 aux did,do
R11298 T20732 T20731 prep of,variety
R11299 T20935 T20936 neg not,do
R11300 T20936 T20916 conj do,were
R11301 T20937 T20938 advmod so,[
R11302 T20733 T20734 amod proinflammatory,cytokines
R11303 T20938 T20940 quantmod [,]
R11304 T20939 T20940 nummod 13,]
R11305 T20940 T20936 dobj ],do
R11306 T20734 T20732 pobj cytokines,of
R11307 T20941 T20916 punct .,were
R11308 T20942 T20944 mark Although,were
R11309 T20943 T20944 expl there,were
R11310 T20735 T20734 cc and,cytokines
R11311 T20944 T20957 advcl were,exert
R11312 T20945 T20946 det no,data
R11313 T20736 T20734 conj chemokines,cytokines
R11314 T20737 T20734 punct ",",cytokines
R11315 T20738 T20740 nsubj which,activate
R11316 T20739 T20740 advmod also,activate
R11317 T20946 T20944 attr data,were
R11318 T20947 T20950 mark that,induces
R11319 T20948 T20950 nsubj MCP-1,induces
R11320 T20740 T20734 relcl activate,cytokines
R11321 T20949 T20950 advmod directly,induces
R11322 T20950 T20957 advcl induces,exert
R11323 T20951 T20953 compound T,activation
R11324 T20952 T20953 compound cell,activation
R11325 T20953 T20950 dobj activation,induces
R11326 T20741 T20742 amod other,cells
R11327 T20954 T20957 punct ",",exert
R11328 T20955 T20957 nsubj it,exert
R11329 T20956 T20957 aux might,exert
R11330 T20742 T20740 dobj cells,activate
R11331 T20957 T20957 ROOT exert,exert
R11332 T20958 T20957 dobj effects,exert
R11333 T20743 T20740 prep in,activate
R11334 T20959 T20957 advmod indirectly,exert
R11335 T20960 T20957 prep on,exert
R11336 T20961 T20962 compound T,cells
R11337 T20744 T20745 det the,joints
R11338 T20962 T20960 pobj cells,on
R11339 T20963 T20957 prep through,exert
R11340 T20964 T20965 det the,activation
R11341 T20745 T20743 pobj joints,in
R11342 T20965 T20963 pobj activation,through
R11343 T20966 T20965 prep of,activation
R11344 T20967 T20966 pobj monocytes/macrophages,of
R11345 T20746 T20729 punct .,produce
R11346 T20968 T20967 prep in,monocytes/macrophages
R11347 T20969 T20970 compound PBMC,cultures
R11348 T20970 T20968 pobj cultures,in
R11349 T20747 T20751 prep For,stimulates
R11350 T20971 T20957 punct .,exert
R11351 T20972 T20973 mark As,reported
R11352 T20973 T20989 advcl reported,increases
R11353 T20748 T20747 pobj example,For
R11354 T20974 T20973 prep for,reported
R11355 T20975 T20976 amod normal,individuals
R11356 T20976 T20974 pobj individuals,for
R11357 T20749 T20751 punct ",",stimulates
R11358 T20977 T20979 nmod [,]
R11359 T20978 T20979 nummod 25,]
R11360 T20979 T20989 nsubj ],increases
R11361 T20750 T20751 nsubj IL-17,stimulates
R11362 T20980 T20979 punct ",",]
R11363 T20981 T20983 compound T,activation
R11364 T20982 T20983 compound cell,activation
R11365 T20751 T20751 ROOT stimulates,stimulates
R11366 T20983 T20979 appos activation,]
R11367 T20984 T20983 prep through,activation
R11368 T21043 T21046 nsubjpass pathway,involved
R11369 T20985 T20987 amod anti-CD3,anti-CD28
R11370 T20986 T20987 compound /,anti-CD28
R11371 T20987 T20984 pobj anti-CD28,through
R11372 T21044 T21046 auxpass was,involved
R11373 T20988 T20989 advmod also,increases
R11374 T20989 T20989 ROOT increases,increases
R11375 T20990 T20991 compound IL-17,induction
R11376 T20991 T20989 dobj induction,increases
R11377 T20992 T20991 prep in,induction
R11378 T20993 T20994 compound RA,PBMC
R11379 T20994 T20992 pobj PBMC,in
R11380 T21045 T21046 advmod mainly,involved
R11381 T20995 T20989 punct .,increases
R11382 T20996 T21009 mark Although,documented
R11383 T20997 T20999 det the,pathway
R11384 T21046 T21040 ccomp involved,examined
R11385 T20998 T20999 amod signaling,pathway
R11386 T20999 T21009 nsubjpass pathway,documented
R11387 T21000 T20999 prep for,pathway
R11388 T21001 T21002 det the,induction
R11389 T21047 T21046 prep in,involved
R11390 T21002 T21000 pobj induction,for
R11391 T21003 T21002 prep of,induction
R11392 T21004 T21003 pobj cytokines/chemokines,of
R11393 T21048 T21049 det the,induction
R11394 T21005 T21002 prep by,induction
R11395 T21049 T21047 pobj induction,in
R11396 T21006 T21005 pobj IL-17,by
R11397 T21007 T21009 aux has,documented
R11398 T21050 T21049 prep of,induction
R11399 T21008 T21009 auxpass been,documented
R11400 T21009 T21018 advcl documented,been
R11401 T21010 T21009 advmod widely,documented
R11402 T21051 T21050 pobj IL-17,of
R11403 T21011 T21013 compound [,]
R11404 T21012 T21013 compound "8,30,31",]
R11405 T21052 T21049 prep in,induction
R11406 T21013 T21009 oprd ],documented
R11407 T21014 T21018 punct ",",been
R11408 T21015 T21016 det no,data
R11409 T21053 T21054 compound RA,PBMC
R11410 T21016 T21018 nsubj data,been
R11411 T21017 T21018 aux have,been
R11412 T21018 T21018 ROOT been,been
R11413 T21054 T21052 pobj PBMC,in
R11414 T21019 T21018 acomp available,been
R11415 T21020 T21019 prep on,available
R11416 T21021 T21026 advmod how,regulated
R11417 T21055 T21040 punct .,examined
R11418 T21022 T21023 compound IL-17,production
R11419 T21023 T21026 nsubjpass production,regulated
R11420 T21024 T21026 aux can,regulated
R11421 T21056 T21057 nsubj We,identified
R11422 T21025 T21026 auxpass be,regulated
R11423 T21026 T21020 pcomp regulated,on
R11424 T21027 T21026 agent by,regulated
R11425 T21028 T21030 amod certain,pathways
R11426 T21029 T21030 compound signaling,pathways
R11427 T21030 T21027 pobj pathways,by
R11428 T21057 T21057 ROOT identified,identified
R11429 T21031 T21018 punct .,been
R11430 T21032 T21040 prep By,examined
R11431 T21033 T21032 pcomp using,By
R11432 T21034 T21036 compound signal,inhibitors
R11433 T21058 T21067 mark that,hampered
R11434 T21035 T21036 compound transduction,inhibitors
R11435 T21036 T21033 dobj inhibitors,using
R11436 T21037 T21040 punct ",",examined
R11437 T21059 T21061 amod anti-CD3-induced,production
R11438 T21038 T21040 nsubj we,examined
R11439 T21039 T21040 advmod therefore,examined
R11440 T21040 T21040 ROOT examined,examined
R11441 T21060 T21061 compound IL-17,production
R11442 T21041 T21043 det which,pathway
R11443 T21042 T21043 amod signaling,pathway
R11444 T21061 T21067 nsubjpass production,hampered
R11445 T21062 T21061 prep in,production
R11446 T21063 T21064 compound RA,PBMC
R11447 T21140 T21128 conj stimulate,activated
R11448 T21141 T21140 dobj NF-κB,stimulate
R11449 T21064 T21062 pobj PBMC,in
R11450 T21142 T21140 prep by,stimulate
R11451 T21143 T21144 det a,mechanism
R11452 T21065 T21067 auxpass was,hampered
R11453 T21144 T21142 pobj mechanism,by
R11454 T21145 T21144 acl involving,mechanism
R11455 T21066 T21067 advmod significantly,hampered
R11456 T21067 T21057 ccomp hampered,identified
R11457 T21068 T21067 agent by,hampered
R11458 T21146 T21149 nmod histone,protein
R11459 T21069 T21071 det the,inhibitor
R11460 T21147 T21149 nmod acetylase,protein
R11461 T21148 T21149 compound p300/CREB-binding,protein
R11462 T21149 T21145 dobj protein,involving
R11463 T21070 T21071 compound PI3K,inhibitor
R11464 T21150 T21152 nmod [,]
R11465 T21151 T21152 nummod 33,]
R11466 T21071 T21068 pobj inhibitor,by
R11467 T21152 T21149 appos ],protein
R11468 T21153 T21140 punct .,stimulate
R11469 T21154 T21155 poss Our,results
R11470 T21072 T21071 appos LY294002,inhibitor
R11471 T21155 T21156 nsubj results,revealed
R11472 T21156 T21156 ROOT revealed,revealed
R11473 T21157 T21163 mark that,affected
R11474 T21158 T21160 compound p38,activation
R11475 T21159 T21160 compound MAPK,activation
R11476 T21160 T21163 nsubjpass activation,affected
R11477 T21073 T21072 cc and,LY294002
R11478 T21161 T21163 auxpass was,affected
R11479 T21074 T21076 det the,inhibitor
R11480 T21162 T21163 neg not,affected
R11481 T21163 T21156 ccomp affected,revealed
R11482 T21075 T21076 compound NF-κB,inhibitor
R11483 T21164 T21163 agent by,affected
R11484 T21165 T21164 pobj LY294002,by
R11485 T21166 T21163 punct ",",affected
R11486 T21076 T21078 dep inhibitor,to
R11487 T21167 T21172 mark whereas,decreased
R11488 T21168 T21170 nmod NF-κB,activity
R11489 T21169 T21170 amod binding,activity
R11490 T21077 T21076 appos PDTC,inhibitor
R11491 T21170 T21172 nsubjpass activity,decreased
R11492 T21171 T21172 auxpass was,decreased
R11493 T21078 T21067 prep to,hampered
R11494 T21172 T21163 advcl decreased,affected
R11495 T21173 T21172 agent by,decreased
R11496 T21174 T21173 pobj LY294002,by
R11497 T21079 T21080 amod comparable,levels
R11498 T21175 T21174 punct ",",LY294002
R11499 T21176 T21177 nsubj which,provided
R11500 T21080 T21078 pobj levels,to
R11501 T21177 T21174 relcl provided,LY294002
R11502 T21178 T21179 det the,evidence
R11503 T21179 T21177 dobj evidence,provided
R11504 T21081 T21080 prep of,levels
R11505 T21180 T21179 prep for,evidence
R11506 T21181 T21184 det a,pathway
R11507 T21082 T21083 amod basal,production
R11508 T21182 T21184 amod p38,pathway
R11509 T21183 T21184 compound MAPK,pathway
R11510 T21184 T21180 pobj pathway,for
R11511 T21185 T21184 amod independent,pathway
R11512 T21083 T21081 pobj production,of
R11513 T21186 T21185 prep of,independent
R11514 T21187 T21188 nummod PI3K,activation
R11515 T21084 T21067 prep without,hampered
R11516 T21188 T21186 pobj activation,of
R11517 T21189 T21156 punct .,revealed
R11518 T21190 T21192 det The,relationship
R11519 T21085 T21084 pobj stimulation,without
R11520 T21191 T21192 amod direct,relationship
R11521 T21192 T21200 nsubj relationship,needs
R11522 T21193 T21192 prep between,relationship
R11523 T21086 T21057 punct .,identified
R11524 T21194 T21193 pobj p38,between
R11525 T21195 T21194 cc and,p38
R11526 T21196 T21194 conj NF-κB,p38
R11527 T21197 T21196 prep for,NF-κB
R11528 T21198 T21199 compound IL-17,production
R11529 T21199 T21197 pobj production,for
R11530 T21087 T21089 nsubj We,found
R11531 T21200 T21200 ROOT needs,needs
R11532 T21201 T21203 aux to,studied
R11533 T21088 T21089 advmod also,found
R11534 T21202 T21203 auxpass be,studied
R11535 T21203 T21200 xcomp studied,needs
R11536 T21204 T21203 prep in,studied
R11537 T21205 T21206 amod future,experiments
R11538 T21089 T21089 ROOT found,found
R11539 T21206 T21204 pobj experiments,in
R11540 T21207 T21200 punct .,needs
R11541 T21090 T21094 mark that,was
R11542 T21208 T21209 det The,search
R11543 T21209 T21216 nsubj search,seemed
R11544 T21210 T21209 prep for,search
R11545 T21091 T21093 amod anti-CD3-induced,production
R11546 T21211 T21213 det a,pathway
R11547 T21212 T21213 amod downstream,pathway
R11548 T21213 T21210 pobj pathway,for
R11549 T21092 T21093 compound IL-17,production
R11550 T21214 T21213 prep of,pathway
R11551 T21215 T21214 pobj PI3K,of
R11552 T21216 T21216 ROOT seemed,seemed
R11553 T21093 T21094 nsubj production,was
R11554 T21217 T21218 aux to,have
R11555 T21218 T21216 xcomp have,seemed
R11556 T21094 T21095 auxpass was,downregulated
R11557 T21219 T21221 det a,response
R11558 T21220 T21221 amod maximal,response
R11559 T21221 T21218 dobj response,have
R11560 T21095 T21089 ccomp downregulated,found
R11561 T21222 T21221 prep of,response
R11562 T21223 T21224 compound Akt,activation
R11563 T21096 T21095 agent by,downregulated
R11564 T21224 T21222 pobj activation,of
R11565 T21225 T21218 prep at,have
R11566 T21226 T21227 nummod 1,hour
R11567 T21227 T21225 pobj hour,at
R11568 T21097 T21098 det the,addition
R11569 T21228 T21227 cc and,hour
R11570 T21229 T21231 det a,loss
R11571 T21098 T21096 pobj addition,by
R11572 T21230 T21231 amod gradual,loss
R11573 T21231 T21227 conj loss,hour
R11574 T21232 T21231 prep of,loss
R11575 T21233 T21232 pobj activity,of
R11576 T21234 T21231 prep at,loss
R11577 T21235 T21236 nummod 2,hours
R11578 T21099 T21098 prep of,addition
R11579 T21236 T21234 pobj hours,at
R11580 T21100 T21099 pobj SB203580,of
R11581 T21101 T21100 punct ",",SB203580
R11582 T21102 T21105 det a,inhibitor
R11583 T21237 T21216 punct .,seemed
R11584 T21238 T21239 det The,fact
R11585 T21103 T21105 amod p38,inhibitor
R11586 T21239 T21239 ROOT fact,fact
R11587 T21104 T21105 compound MAPK,inhibitor
R11588 T21240 T21242 mark that,is
R11589 T21241 T21242 nsubj Akt,is
R11590 T21105 T21100 appos inhibitor,SB203580
R11591 T21106 T21089 punct .,found
R11592 T21107 T21108 nsubj It,is
R11593 T21242 T21239 acl is,fact
R11594 T21108 T21108 ROOT is,is
R11595 T21243 T21242 acomp phosphorylated,is
R11596 T21244 T21243 prep upon,phosphorylated
R11597 T21245 T21246 amod anti-CD3,stimulation
R11598 T21109 T21108 acomp interesting,is
R11599 T21246 T21244 pobj stimulation,upon
R11600 T21247 T21243 auxpass suggests,phosphorylated
R11601 T21248 T21250 det the,involvement
R11602 T21110 T21115 mark that,supports
R11603 T21249 T21250 amod possible,involvement
R11604 T21250 T21247 dobj involvement,suggests
R11605 T21111 T21112 det a,series
R11606 T21251 T21250 prep of,involvement
R11607 T21252 T21251 pobj PI3K,of
R11608 T21253 T21250 prep in,involvement
R11609 T21254 T21255 det the,induction
R11610 T21112 T21115 nsubj series,supports
R11611 T21255 T21253 pobj induction,in
R11612 T21256 T21255 prep of,induction
R11613 T21257 T21256 pobj IL-17,of
R11614 T21113 T21112 prep of,series
R11615 T21258 T21255 prep in,induction
R11616 T21259 T21258 pobj RA,in
R11617 T21260 T21239 punct .,fact
R11618 T21114 T21113 pobj evidence,of
R11619 T21261 T21284 prep In,is
R11620 T21262 T21261 pobj view,In
R11621 T21263 T21262 prep of,view
R11622 T21115 T21108 ccomp supports,is
R11623 T21264 T21265 det the,fact
R11624 T21265 T21263 pobj fact,of
R11625 T21116 T21115 dobj crosstalk,supports
R11626 T21266 T21270 mark that,activated
R11627 T21267 T21270 nsubjpass NF-κB,activated
R11628 T21268 T21270 auxpass was,activated
R11629 T21269 T21270 advmod also,activated
R11630 T21270 T21265 acl activated,fact
R11631 T21271 T21270 agent by,activated
R11632 T21272 T21274 amod anti-CD3,anti-CD28
R11633 T21117 T21116 prep between,crosstalk
R11634 T21273 T21274 compound /,anti-CD28
R11635 T21274 T21271 pobj anti-CD28,by
R11636 T21275 T21274 punct ",",anti-CD28
R11637 T21276 T21274 conj IL-15,anti-CD28
R11638 T21118 T21117 pobj NF-κB,between
R11639 T21277 T21276 cc or,IL-15
R11640 T21119 T21118 cc and,NF-κB
R11641 T21120 T21118 conj p38,NF-κB
R11642 T21121 T21108 punct .,is
R11643 T21278 T21276 conj mitogens,IL-15
R11644 T21279 T21278 prep in,mitogens
R11645 T21280 T21281 poss our,experiments
R11646 T21281 T21279 pobj experiments,in
R11647 T21122 T21128 prep In,activated
R11648 T21282 T21284 punct ",",is
R11649 T21283 T21284 nsubj it,is
R11650 T21123 T21122 pobj myocytes,In
R11651 T21284 T21284 ROOT is,is
R11652 T21285 T21286 advmod most,likely
R11653 T21286 T21284 acomp likely,is
R11654 T21124 T21128 punct ",",activated
R11655 T21287 T21294 mark that,involved
R11656 T21288 T21290 det the,pathway
R11657 T21289 T21290 compound NF-κB,pathway
R11658 T21290 T21294 nsubjpass pathway,involved
R11659 T21125 T21126 compound IκB,kinase-β
R11660 T21291 T21294 auxpass is,involved
R11661 T21292 T21294 advmod also,involved
R11662 T21293 T21294 advmod actively,involved
R11663 T21126 T21128 nsubjpass kinase-β,activated
R11664 T21294 T21286 ccomp involved,likely
R11665 T21295 T21294 prep in,involved
R11666 T21296 T21297 det the,induction
R11667 T21127 T21128 auxpass is,activated
R11668 T21297 T21295 pobj induction,in
R11669 T21298 T21297 prep of,induction
R11670 T21299 T21298 pobj IL-17,of
R11671 T21128 T21128 ROOT activated,activated
R11672 T21300 T21297 prep in,induction
R11673 T21301 T21302 compound RA,PBMC
R11674 T21302 T21300 pobj PBMC,in
R11675 T21129 T21128 agent by,activated
R11676 T21303 T21284 punct .,is
R11677 T21304 T21322 prep In,activated
R11678 T21305 T21304 pobj contrast,In
R11679 T21306 T21322 punct ",",activated
R11680 T21307 T21311 det the,pathway
R11681 T21308 T21311 compound AP-1,pathway
R11682 T21130 T21133 nummod p38,]
R11683 T21309 T21311 compound signal,pathway
R11684 T21310 T21311 compound transduction,pathway
R11685 T21311 T21322 nsubjpass pathway,activated
R11686 T21312 T21311 punct ",",pathway
R11687 T21131 T21133 nmod [,]
R11688 T21313 T21316 det another,pathway
R11689 T21314 T21316 amod important,pathway
R11690 T21132 T21133 nummod 32,]
R11691 T21315 T21316 amod signaling,pathway
R11692 T21316 T21311 conj pathway,pathway
R11693 T21317 T21316 prep for,pathway
R11694 T21133 T21129 pobj ],by
R11695 T21318 T21317 pobj cytokines/chemokines,for
R11696 T21134 T21128 punct ",",activated
R11697 T21135 T21128 cc and,activated
R11698 T21319 T21322 punct ",",activated
R11699 T21320 T21322 auxpass was,activated
R11700 T21136 T21138 det the,p38
R11701 T21321 T21322 neg not,activated
R11702 T21322 T21322 ROOT activated,activated
R11703 T21323 T21322 prep in,activated
R11704 T21137 T21138 amod activated,p38
R11705 T21324 T21325 poss our,experiments
R11706 T21325 T21323 pobj experiments,in
R11707 T21326 T21325 punct (,experiments
R11708 T21138 T21140 nsubj p38,stimulate
R11709 T21327 T21329 nsubj data,shown
R11710 T21328 T21329 neg not,shown
R11711 T21139 T21140 aux can,stimulate
R11712 T21329 T21322 conj shown,activated
R11713 T21330 T21329 punct ),shown
R11714 T21331 T21322 punct .,activated
R11715 T21332 T21345 mark Although,reported
R11716 T21334 T21333 cc and,PI3K
R11717 T21333 T21345 nsubjpass PI3K,reported
R11718 T21335 T21337 poss its,kinase
R11719 T21431 T21432 nsubj IL-17,signaling
R11720 T21432 T21434 nsubj signaling,is
R11721 T21433 T21434 nsubj pathway,is
R11722 T21336 T21337 amod downstream,kinase
R11723 T21434 T21429 ccomp is,understanding
R11724 T21435 T21437 det an,element
R11725 T21436 T21437 amod important,element
R11726 T21437 T21434 attr element,is
R11727 T21438 T21437 prep of,element
R11728 T21439 T21438 pcomp developing,of
R11729 T21440 T21442 amod new,therapies
R11730 T21337 T21338 compound kinase,Akt
R11731 T21441 T21442 amod targeted,therapies
R11732 T21442 T21439 dobj therapies,developing
R11733 T21443 T21439 prep in,developing
R11734 T21444 T21443 pobj RA,in
R11735 T21338 T21333 conj Akt,PI3K
R11736 T21445 T21410 punct .,Considering
R11737 T21339 T21333 prep in,PI3K
R11738 T21340 T21339 pobj association,in
R11739 T21341 T21340 prep with,association
R11740 T21342 T21341 pobj NF-κB,with
R11741 T21343 T21345 aux have,reported
R11742 T21344 T21345 auxpass been,reported
R11743 T21345 T21363 advcl reported,lacking
R11744 T21346 T21347 aux to,deliver
R11745 T21347 T21345 xcomp deliver,reported
R11746 T21348 T21349 amod activating,signals
R11747 T21349 T21347 dobj signals,deliver
R11750 T21350 T21347 prep in,deliver
R11752 T21351 T21353 amod many,types
R11753 T21352 T21353 compound cell,types
R11754 T21353 T21350 pobj types,in
R11755 T21354 T21363 punct ",",lacking
R11756 T21355 T21356 det the,data
R11757 T21356 T21363 nsubj data,lacking
R11759 T21357 T21356 prep on,data
R11761 T21358 T21359 det the,signal
R11762 T21359 T21357 pobj signal,on
R11764 T21360 T21359 acl inducing,signal
R11766 T21361 T21360 dobj IL-17,inducing
R11767 T21362 T21363 aux are,lacking
R11770 T21363 T21363 ROOT lacking,lacking
R11772 T21364 T21363 punct .,lacking
R11775 T21365 T21366 poss Our,data
R11780 T21366 T21368 nsubj data,demonstrated
R11781 T21367 T21368 advmod clearly,demonstrated
R11783 T21368 T21368 ROOT demonstrated,demonstrated
R11786 T21369 T21376 mark that,be
R11789 T21370 T21376 nsubj PI3K/Akt,be
R11793 T21371 T21370 cc and,PI3K/Akt
R11794 T21372 T21374 amod resultant,activation
R11795 T21373 T21374 compound NF-κB,activation
R11797 T21374 T21370 conj activation,PI3K/Akt
R11800 T21375 T21376 aux could,be
R11811 T21376 T21368 ccomp be,demonstrated
R11813 T21377 T21379 det an,arbitrator
R11814 T21378 T21379 amod important,arbitrator
R11817 T21379 T21376 attr arbitrator,be
R11821 T21380 T21379 prep of,arbitrator
R11822 T21381 T21382 det the,upregulation
R11823 T21382 T21380 pobj upregulation,of
R11828 T21383 T21382 prep of,upregulation
R11831 T21384 T21383 pobj IL-17,of
R11833 T21385 T21382 prep in,upregulation
R11835 T21386 T21385 pobj RA,in
R11840 T21387 T21379 punct ",",arbitrator
R11841 T21388 T21379 prep on,arbitrator
R11843 T21389 T21390 det the,basis
R11844 T21390 T21388 pobj basis,on
R11845 T21391 T21390 prep of,basis
R11846 T21392 T21393 poss our,experiments
R11848 T21393 T21391 pobj experiments,of
R11849 T21394 T21393 acl showing,experiments
R11853 T21395 T21396 amod simultaneous,blocking
R11855 T21396 T21394 dobj blocking,showing
R11858 T21397 T21396 prep of,blocking
R11860 T21398 T21400 amod NF-κB,activity
R11862 T21399 T21400 amod binding,activity
R11864 T21400 T21397 pobj activity,of
R11866 T21401 T21400 prep in,activity
R11868 T21402 T21404 det the,promoter
R11871 T21403 T21404 compound IL-17,promoter
R11872 T21404 T21401 pobj promoter,in
R11874 T21405 T21396 prep by,blocking
R11883 T21406 T21405 pobj PDTC,by
R11884 T21407 T21406 cc and,PDTC
R11887 T21408 T21406 conj LY294002,PDTC
R11890 T21409 T21368 punct .,demonstrated
R11893 T21410 T21410 ROOT Considering,Considering
R11895 T21411 T21413 poss its,activities
R11898 T21412 T21413 amod proinflammatory,activities
R11900 T21413 T21410 dobj activities,Considering
R11902 T21414 T21413 cc and,activities
R11903 T21415 T21416 amod successful,induction
R11908 T21416 T21413 conj induction,activities
R11914 T21417 T21416 prep of,induction
R11916 T21418 T21417 pobj anti-IL-17,of
R11917 T21419 T21418 prep for,anti-IL-17
R11918 T21420 T21419 pcomp ameliorating,for
R11919 T21421 T21420 dobj arthritis,ameliorating
R11920 T21422 T21420 prep in,ameliorating
R11921 T21423 T21424 compound animal,models
R11922 T21424 T21422 pobj models,in
R11923 T21425 T21427 compound [,]
R11926 T21426 T21427 compound "2,6,34-36",]
R11927 T21427 T21424 appos ],models
R11928 T21428 T21427 punct ",",]
R11929 T21429 T21420 conj understanding,ameliorating
R11930 T21430 T21431 det the,IL-17
R12228 T22443 T22445 nsubj We,detected
R12229 T22444 T22445 aux have,detected
R12230 T22445 T22445 ROOT detected,detected
R12231 T22446 T22449 det a,production
R12232 T22447 T22448 advmod more,pronounced
R12233 T22448 T22449 amod pronounced,production
R12234 T22449 T22445 dobj production,detected
R12235 T22450 T22449 prep of,production
R12236 T22451 T22450 pobj IL-17,of
R12237 T22452 T22449 prep from,production
R12238 T22453 T22454 compound RA,PBMC
R12239 T22454 T22452 pobj PBMC,from
R12240 T22455 T22449 prep in,production
R12241 T22456 T22455 pobj response,in
R12242 T22457 T22456 prep to,response
R12243 T22458 T22457 pobj IL-15,to
R12244 T22459 T22458 cc and,IL-15
R12245 T22460 T22458 conj MCP-1,IL-15
R12246 T22461 T22463 advmod as,as
R12247 T22462 T22463 advmod well,as
R12248 T22463 T22456 cc as,response
R12249 T22464 T22456 conj stimulation,response
R12250 T22465 T22449 prep by,production
R12251 T22466 T22468 amod anti-CD3,anti-CD28
R12252 T22467 T22468 compound /,anti-CD28
R12253 T22468 T22465 pobj anti-CD28,by
R12254 T22469 T22445 punct .,detected
R12255 T22470 T22473 nsubj We,shown
R12256 T22471 T22473 aux have,shown
R12257 T22472 T22473 advmod also,shown
R12258 T22473 T22473 ROOT shown,shown
R12259 T22474 T22487 mark that,be
R12260 T22475 T22487 nsubj upregulation,be
R12261 T22476 T22475 prep of,upregulation
R12262 T22477 T22476 pobj IL-17,of
R12263 T22478 T22475 prep by,upregulation
R12264 T22479 T22481 amod activated,cells
R12265 T22480 T22481 compound T,cells
R12266 T22481 T22478 pobj cells,by
R12267 T22482 T22475 prep in,upregulation
R12268 T22483 T22482 pobj patients,in
R12269 T22484 T22475 prep with,upregulation
R12270 T22485 T22484 pobj RA,with
R12271 T22486 T22487 aux could,be
R12272 T22487 T22473 ccomp be,shown
R12273 T22488 T22489 det the,result
R12274 T22489 T22487 attr result,be
R12275 T22490 T22489 prep of,result
R12276 T22491 T22490 pobj activation,of
R12277 T22492 T22487 prep via,be
R12278 T22493 T22495 det the,pathway
R12279 T22494 T22495 amod PI3K/Akt,pathway
R12280 T22495 T22492 pobj pathway,via
R12281 T22496 T22495 prep with,pathway
R12282 T22497 T22499 amod resultant,activation
R12283 T22498 T22499 compound NF-κB,activation
R12284 T22499 T22496 pobj activation,with
R12285 T22500 T22473 punct .,shown
R12286 T22501 T22502 poss Our,data
R12287 T22502 T22503 nsubj data,provide
R12288 T22503 T22503 ROOT provide,provide
R12289 T22504 T22503 dobj insights,provide
R12290 T22505 T22504 prep into,insights
R12291 T22506 T22507 amod cellular,mechanisms
R12292 T22507 T22505 pobj mechanisms,into
R12293 T22508 T22507 prep of,mechanisms
R12294 T22509 T22510 det the,regulation
R12295 T22510 T22508 pobj regulation,of
R12296 T22511 T22510 prep of,regulation
R12297 T22512 T22513 amod IL-17,production
R12298 T22513 T22511 pobj production,of
R12299 T22514 T22513 prep in,production
R12300 T22515 T22514 pobj RA,in
R12301 T22516 T22503 punct ",",provide
R12302 T22517 T22503 cc and,provide
R12303 T22518 T22503 conj highlight,provide
R12304 T22519 T22520 det the,role
R12305 T22520 T22518 dobj role,highlight
R12306 T22521 T22520 prep of,role
R12307 T22522 T22523 compound T,cells
R12308 T22523 T22521 pobj cells,of
R12309 T22524 T22523 punct ",",cells
R12310 T22525 T22529 nsubj which,neglected
R12311 T22526 T22529 aux has,neglected
R12312 T22527 T22529 advmod hitherto,neglected
R12313 T22528 T22529 auxpass been,neglected
R12314 T22529 T22523 relcl neglected,cells
R12315 T22530 T22529 prep in,neglected
R12316 T22531 T22532 compound RA,pathogenesis
R12317 T22532 T22530 pobj pathogenesis,in
R12318 T22533 T22503 punct .,provide
R12319 T22534 T22535 advmod Together,with
R12320 T22535 T22550 prep with,added
R12321 T22536 T22537 amod recent,data
R12322 T22537 T22535 pobj data,with
R12323 T22538 T22537 prep on,data
R12324 T22539 T22541 det the,introduction
R12325 T22540 T22541 amod successful,introduction
R12326 T22541 T22538 pobj introduction,on
R12327 T22542 T22541 prep of,introduction
R12328 T22543 T22542 pobj anti-IL-17,of
R12329 T22544 T22541 prep in,introduction
R12330 T22545 T22544 pobj RA,in
R12331 T22546 T22550 punct ",",added
R12332 T22547 T22548 poss our,results
R12333 T22548 T22550 nsubj results,added
R12334 T22549 T22550 aux have,added
R12335 T22550 T22550 ROOT added,added
R12336 T22551 T22550 dobj information,added
R12337 T22552 T22550 prep for,added
R12338 T22553 T22556 det the,targeting
R12339 T22554 T22556 amod future,targeting
R12340 T22555 T22556 amod molecular,targeting
R12341 T22556 T22552 pobj targeting,for
R12342 T22557 T22556 prep of,targeting
R12343 T22558 T22560 amod new,applications
R12344 T22559 T22560 amod therapeutic,applications
R12345 T22560 T22557 pobj applications,of
R12346 T22561 T22560 prep in,applications
R12347 T22562 T22561 pobj RA,in
R12348 T22563 T22550 punct .,added
R1821 T3852 T3853 amod Rheumatoid,arthritis
R1822 T3853 T3858 nsubjpass arthritis,characterized
R1823 T3854 T3853 punct (,arthritis
R1824 T3855 T3853 appos RA,arthritis
R1828 T3856 T3853 punct ),arthritis
R1829 T3857 T3858 auxpass is,characterized
R1832 T3858 T3858 ROOT characterized,characterized
R1833 T3859 T3858 agent by,characterized
R1836 T3860 T3859 pobj infiltrations,by
R1838 T3861 T3860 prep of,infiltrations
R1841 T3862 T3861 pobj macrophages,of
R1843 T3863 T3862 cc and,macrophages
R1845 T3864 T3865 compound T,cells
R1846 T3865 T3862 conj cells,macrophages
R1847 T3866 T3858 prep into,characterized
R1848 T3867 T3868 det the,joint
R1849 T3868 T3872 amod joint,hyperplasia
R1850 T3869 T3868 punct ",",joint
R1851 T3948 T3964 advmod ago,was
R1852 T3870 T3868 cc and,joint
R1853 T3949 T3964 punct ",",was
R1854 T3950 T3951 det the,primacy
R1855 T3871 T3868 conj synovial,joint
R1856 T3951 T3964 nsubj primacy,was
R1857 T3952 T3951 prep of,primacy
R1858 T3872 T3866 pobj hyperplasia,into
R1859 T3953 T3954 compound T,cells
R1860 T3954 T3952 pobj cells,of
R1861 T3873 T3858 punct .,characterized
R1862 T3955 T3951 prep in,primacy
R1863 T3956 T3957 det the,pathogenesis
R1864 T3957 T3955 pobj pathogenesis,in
R1865 T3874 T3875 amod Proinflammatory,cytokines
R1866 T3958 T3957 prep of,pathogenesis
R1867 T3959 T3960 amod autoimmune,disease
R1868 T3960 T3958 pobj disease,of
R1869 T3875 T3881 nsubjpass cytokines,known
R1870 T3961 T3962 amod such,as
R1871 T3962 T3957 prep as,pathogenesis
R1872 T3963 T3964 nsubj RA,was
R1873 T3876 T3875 acl released,cytokines
R1874 T3964 T3964 ROOT was,was
R1875 T3965 T3964 acomp undisputed,was
R1876 T3966 T3968 mark because,are
R1877 T3967 T3968 nsubj they,are
R1878 T3877 T3876 prep from,released
R1879 T3968 T3964 advcl are,was
R1880 T3878 T3879 det these,cells
R1881 T3969 T3972 det the,population
R1882 T3970 T3972 amod largest,population
R1883 T3879 T3877 pobj cells,from
R1884 T3971 T3972 compound cell,population
R1885 T3972 T3968 attr population,are
R1886 T3973 T3972 acl infiltrating,population
R1887 T3880 T3881 auxpass are,known
R1888 T3974 T3975 det the,synovium
R1889 T3975 T3973 dobj synovium,infiltrating
R1890 T3976 T3964 punct .,was
R1891 T3881 T3881 ROOT known,known
R1892 T3977 T3983 advmod However,demonstrated
R1893 T3978 T3983 punct ",",demonstrated
R1894 T3882 T3883 aux to,be
R1895 T3979 T3980 det a,series
R1896 T3980 T3983 nsubj series,demonstrated
R1897 T3981 T3980 prep of,series
R1898 T3883 T3881 xcomp be,known
R1899 T3982 T3981 pobj studies,of
R1900 T3983 T3983 ROOT demonstrated,demonstrated
R1901 T3884 T3883 acomp important,be
R1902 T3984 T3983 dobj paucity,demonstrated
R1903 T3985 T3984 prep of,paucity
R1904 T3986 T3988 nmod T,cytokines
R1905 T3885 T3883 prep in,be
R1906 T3987 T3988 amod cell-derived,cytokines
R1907 T3988 T3985 pobj cytokines,of
R1908 T3989 T3990 amod such,as
R1909 T3886 T3887 det the,destruction
R1910 T3990 T3988 prep as,cytokines
R1911 T3991 T3990 pobj IL-2,as
R1912 T3992 T3991 cc and,IL-2
R1913 T3887 T3885 pobj destruction,in
R1914 T3993 T3991 conj interferon-γ,IL-2
R1915 T3994 T3983 prep in,demonstrated
R1916 T3995 T3996 det the,joints
R1917 T3996 T3994 pobj joints,in
R1918 T3888 T3887 prep of,destruction
R1919 T3997 T3996 prep of,joints
R1920 T3998 T3997 pobj RA,of
R1921 T3999 T3983 punct ",",demonstrated
R1922 T3889 T3888 pobj joints,of
R1923 T3890 T3887 prep in,destruction
R1924 T4000 T4015 mark whereas,were
R1925 T3891 T3892 compound RA,[
R1926 T4001 T4015 nsubj macrophage,were
R1927 T4002 T4001 cc and,macrophage
R1928 T4003 T4001 conj fibroblast,macrophage
R1929 T3892 T3890 pobj [,in
R1930 T4004 T4001 conj cytokines,macrophage
R1931 T4005 T4004 prep including,cytokines
R1932 T4006 T4005 pobj IL-1,including
R1933 T4007 T4006 punct ",",IL-1
R1934 T3893 T3894 nummod 1,]
R1935 T4008 T4006 conj IL-6,IL-1
R1936 T4009 T4008 punct ",",IL-6
R1937 T4010 T4008 conj IL-15,IL-6
R1938 T4011 T4010 punct ",",IL-15
R1939 T3894 T3892 appos ],[
R1940 T4012 T4010 conj IL-18,IL-15
R1941 T4013 T4012 cc and,IL-18
R1942 T3895 T3881 punct .,known
R1943 T4014 T4012 conj TNF-α,IL-18
R1944 T4015 T3983 advcl were,demonstrated
R1945 T4016 T4015 acomp abundant,were
R1946 T4017 T4016 prep in,abundant
R1947 T3896 T3899 det The,benefits
R1948 T4018 T4019 amod rheumatoid,synovium
R1949 T4019 T4017 pobj synovium,in
R1950 T4020 T3983 punct .,demonstrated
R1951 T3897 T3899 amod favorable,benefits
R1952 T4021 T4022 det This,paradox
R1953 T4022 T4024 nsubj paradox,questioned
R1954 T4023 T4024 aux has,questioned
R1955 T3898 T3899 amod clinical,benefits
R1956 T4024 T4024 ROOT questioned,questioned
R1957 T4025 T4026 det the,role
R1958 T3899 T3911 nsubj benefits,α
R1959 T4026 T4024 dobj role,questioned
R1960 T4027 T4026 prep of,role
R1961 T4028 T4029 compound T,cells
R1962 T3900 T3899 acl obtained,benefits
R1963 T4029 T4027 pobj cells,of
R1964 T4030 T4026 prep in,role
R1965 T4031 T4032 det the,pathogenesis
R1966 T3901 T3900 prep with,obtained
R1967 T4032 T4030 pobj pathogenesis,in
R1968 T4033 T4032 prep of,pathogenesis
R1969 T3902 T3901 pobj inhibitors,with
R1970 T4034 T4035 compound RA,[
R1971 T4035 T4033 pobj [,of
R1972 T4036 T4037 nummod 3,]
R1973 T3903 T3902 prep of,inhibitors
R1974 T4037 T4032 appos ],pathogenesis
R1975 T4038 T4024 punct .,questioned
R1976 T4039 T4043 mark Because,demonstrated
R1977 T3904 T3905 compound tumor,necrosis
R1978 T4040 T4043 nsubj we,demonstrated
R1979 T4041 T4043 aux have,demonstrated
R1980 T4042 T4043 advmod already,demonstrated
R1981 T4043 T4043 ROOT demonstrated,demonstrated
R1982 T4044 T4046 det the,proliferation
R1983 T3905 T3906 compound necrosis,factor
R1984 T3906 T3903 pobj factor,of
R1985 T3907 T3908 punct (,TNF
R1986 T4045 T4046 amod enhanced,proliferation
R1987 T4046 T4043 dobj proliferation,demonstrated
R1988 T3908 T3906 appos TNF,factor
R1989 T4047 T4046 prep of,proliferation
R1990 T3909 T3900 punct ),obtained
R1991 T4048 T4047 pobj antigen,of
R1992 T4049 T4051 amod specific,cells
R1993 T3910 T3899 punct -,benefits
R1994 T4050 T4051 compound T,cells
R1995 T4051 T4047 pobj cells,of
R1996 T4052 T4046 punct ",",proliferation
R1997 T3911 T3911 ROOT α,α
R1998 T4053 T4055 advmod especially,type
R1999 T4054 T4055 aux to,type
R2000 T3912 T3911 punct ),α
R2001 T4055 T4082 advcl type,needs
R2002 T4056 T4057 compound II,collagen
R2003 T4057 T4055 dobj collagen,type
R2004 T3913 T3911 cc and,α
R2005 T4058 T4055 punct ",",type
R2006 T4059 T4055 cc and,type
R2007 T4060 T4061 det the,skewing
R2008 T3914 T3919 nsubj interleukin,suggest
R2009 T4061 T4055 conj skewing,type
R2010 T4062 T4061 prep of,skewing
R2011 T4063 T4064 compound T,helper
R2012 T3915 T3916 punct (,IL
R2013 T4064 T4065 compound helper,type
R2014 T4065 T4061 conj type,skewing
R2015 T3916 T3914 appos IL,interleukin
R2016 T4066 T4065 nummod 1,type
R2017 T4067 T4068 punct (,Th1
R2018 T4068 T4065 appos Th1,type
R2019 T3917 T3918 punct ),-1
R2020 T4069 T4068 punct ),Th1
R2021 T4070 T4065 conj cytokines,type
R2022 T4071 T4070 prep in,cytokines
R2023 T3918 T3919 nsubj -1,suggest
R2024 T4072 T4073 compound RA,[
R2025 T4073 T4071 pobj [,in
R2026 T3919 T3911 conj suggest,α
R2027 T4074 T4075 nummod 4,]
R2028 T4075 T4082 nsubj ],needs
R2029 T4076 T4075 punct ",",]
R2030 T4077 T4078 det the,role
R2031 T4078 T4075 appos role,]
R2032 T4079 T4078 prep of,role
R2033 T3920 T3928 mark that,been
R2034 T4080 T4081 compound T,cells
R2035 T4081 T4079 pobj cells,of
R2036 T4082 T4043 conj needs,demonstrated
R2037 T3921 T3922 det the,blockade
R2038 T4083 T4085 aux to,elucidated
R2039 T4084 T4085 auxpass be,elucidated
R2040 T4085 T4082 xcomp elucidated,needs
R2041 T3922 T3928 nsubj blockade,been
R2042 T4086 T4085 prep in,elucidated
R2043 T4087 T4088 amod different,aspects
R2044 T4088 T4086 pobj aspects,in
R2045 T3923 T3922 prep of,blockade
R2046 T4089 T4082 punct .,needs
R2047 T4090 T4091 nsubj IL-17,is
R2048 T3924 T3926 amod key,cytokines
R2049 T4091 T4091 ROOT is,is
R2050 T4092 T4091 attr one,is
R2051 T4093 T4092 prep of,one
R2052 T3925 T3926 amod inflammatory,cytokines
R2053 T4094 T4096 det the,cytokines
R2054 T4095 T4096 amod inflammatory,cytokines
R2055 T4096 T4093 pobj cytokines,of
R2056 T3926 T3923 pobj cytokines,of
R2057 T4097 T4092 acl secreted,one
R2058 T4098 T4099 advmod mainly,by
R2059 T4099 T4097 agent by,secreted
R2060 T3927 T3928 aux has,been
R2061 T4100 T4102 amod activated,cells
R2062 T4101 T4102 compound T,cells
R2063 T4102 T4099 pobj cells,by
R2064 T3928 T3919 ccomp been,suggest
R2065 T4103 T4102 punct ",",cells
R2066 T4104 T4106 nsubj which,induce
R2067 T4105 T4106 aux can,induce
R2068 T3929 T3931 det the,issue
R2069 T4106 T4102 relcl induce,cells
R2070 T4107 T4106 dobj IL-6,induce
R2071 T3930 T3931 amod important,issue
R2072 T4108 T4107 cc and,IL-6
R2073 T4109 T4107 conj IL-8,IL-6
R2074 T4110 T4106 prep by,induce
R2075 T3931 T3928 attr issue,been
R2076 T4111 T4110 pobj fibroblasts,by
R2077 T4112 T4114 nmod [,]
R2078 T4113 T4114 nummod 5,]
R2079 T3932 T3931 prep in,issue
R2080 T4114 T4106 npadvmod ],induce
R2081 T3933 T3934 det the,development
R2082 T4115 T4091 punct .,is
R2083 T4116 T4117 det This,cytokine
R2084 T3934 T3932 pobj development,in
R2085 T4117 T4118 nsubj cytokine,is
R2086 T4118 T4143 ccomp is,produced
R2087 T3935 T3934 prep of,development
R2088 T4119 T4118 prep of,is
R2089 T4120 T4119 pobj interest,of
R2090 T4121 T4118 prep for,is
R2091 T3936 T3938 amod new,applications
R2092 T4122 T4124 nummod two,reasons
R2093 T4123 T4124 amod major,reasons
R2094 T4124 T4121 pobj reasons,for
R2095 T3937 T3938 amod therapeutic,applications
R2096 T4125 T4124 punct :,reasons
R2097 T3938 T3935 pobj applications,of
R2098 T4126 T4129 advmod first,to
R2099 T4127 T4129 punct ",",to
R2100 T3939 T3941 amod [,]
R2101 T4128 T4129 advmod similarly,to
R2102 T4129 T4118 prep to,is
R2103 T4130 T4129 pobj TNF-α,to
R2104 T3940 T3941 nummod 2,]
R2105 T4131 T4130 cc and,TNF-α
R2106 T4132 T4130 conj IL-1,TNF-α
R2107 T3941 T3931 conj ],issue
R2108 T4133 T4135 punct ",",has
R2109 T4134 T4135 nsubj IL-17,has
R2110 T4135 T4143 ccomp has,produced
R2111 T3942 T3919 punct .,suggest
R2112 T4136 T4137 amod proinflammatory,properties
R2113 T4137 T4135 dobj properties,has
R2114 T4138 T4143 punct ;,produced
R2115 T3943 T3944 det A,little
R2116 T4139 T4143 advmod second,produced
R2117 T4140 T4143 punct ",",produced
R2118 T4141 T4143 nsubjpass it,produced
R2119 T3944 T3948 npadvmod little,ago
R2120 T3945 T3946 quantmod over,a
R2121 T3946 T3947 nummod a,decade
R2122 T3947 T3948 npadvmod decade,ago
R2123 T4142 T4143 auxpass is,produced
R2124 T4143 T4143 ROOT produced,produced
R2125 T4239 T4237 acomp higher,were
R2126 T4144 T4143 agent by,produced
R2127 T4145 T4146 compound T,cells
R2128 T4146 T4144 pobj cells,by
R2129 T4147 T4149 nmod [,]
R2130 T4148 T4149 nummod 6,]
R2131 T4149 T4146 appos ],cells
R2132 T4150 T4143 punct .,produced
R2133 T4151 T4152 amod Recent,observations
R2134 T4240 T4237 prep in,were
R2135 T4152 T4153 nsubj observations,demonstrated
R2136 T4153 T4153 ROOT demonstrated,demonstrated
R2137 T4154 T4158 mark that,activate
R2138 T4241 T4240 pobj patients,in
R2139 T4155 T4158 nsubj IL-17,activate
R2140 T4242 T4241 prep with,patients
R2141 T4156 T4158 aux can,activate
R2142 T4157 T4158 advmod also,activate
R2143 T4158 T4153 ccomp activate,demonstrated
R2144 T4159 T4161 amod osteoclastic,resorption
R2145 T4243 T4242 pobj RA,with
R2146 T4160 T4161 compound bone,resorption
R2147 T4161 T4158 dobj resorption,activate
R2148 T4162 T4158 prep by,activate
R2149 T4244 T4237 prep than,were
R2150 T4163 T4164 det the,induction
R2151 T4164 T4162 pobj induction,by
R2152 T4165 T4164 prep of,induction
R2153 T4245 T4244 prep in,than
R2154 T4166 T4165 pobj RANKL,of
R2155 T4167 T4169 punct (,activator
R2156 T4168 T4169 compound receptor,activator
R2157 T4246 T4245 pobj patients,in
R2158 T4169 T4166 appos activator,RANKL
R2159 T4170 T4169 prep of,activator
R2160 T4171 T4172 amod nuclear,factor
R2161 T4247 T4246 prep with,patients
R2162 T4172 T4170 pobj factor,of
R2163 T4173 T4177 compound κB,ligand
R2164 T4174 T4177 compound [,ligand
R2165 T4248 T4247 pobj osteoarthritis,with
R2166 T4175 T4177 compound NF-κB,ligand
R2167 T4176 T4177 compound ],ligand
R2168 T4177 T4172 appos ligand,factor
R2169 T4249 T4248 punct (,osteoarthritis
R2170 T4178 T4177 punct ),ligand
R2171 T4179 T4172 punct ",",factor
R2172 T4180 T4182 nsubjpass which,involved
R2173 T4250 T4248 appos OA,osteoarthritis
R2174 T4181 T4182 auxpass is,involved
R2175 T4182 T4172 relcl involved,factor
R2176 T4183 T4182 prep in,involved
R2177 T4251 T4248 punct ),osteoarthritis
R2178 T4184 T4185 amod bony,erosion
R2179 T4185 T4183 pobj erosion,in
R2180 T4252 T4237 punct ",",were
R2181 T4186 T4185 prep in,erosion
R2182 T4187 T4188 compound RA,[
R2183 T4188 T4186 pobj [,in
R2184 T4189 T4190 nummod 7,]
R2185 T4190 T4166 conj ],RANKL
R2186 T4191 T4153 punct .,demonstrated
R2187 T4253 T4237 cc and,were
R2188 T4192 T4194 nsubj It,stimulates
R2189 T4193 T4194 advmod also,stimulates
R2190 T4194 T4194 ROOT stimulates,stimulates
R2191 T4195 T4196 det the,production
R2192 T4254 T4256 nsubjpass it,produced
R2193 T4196 T4194 dobj production,stimulates
R2194 T4197 T4196 prep of,production
R2195 T4198 T4197 pobj IL-6,of
R2196 T4255 T4256 auxpass was,produced
R2197 T4199 T4198 cc and,IL-6
R2198 T4200 T4202 nmod leukemia,factor
R2199 T4201 T4202 amod inhibitory,factor
R2200 T4256 T4237 conj produced,were
R2201 T4202 T4196 conj factor,production
R2202 T4257 T4256 agent by,produced
R2203 T4203 T4196 prep by,production
R2204 T4258 T4261 compound CD4,cells
R2205 T4204 T4203 pobj synoviocytes,by
R2206 T4205 T4194 punct ",",stimulates
R2207 T4259 T4261 compound +,cells
R2208 T4206 T4194 cc and,stimulates
R2209 T4207 T4194 conj of,stimulates
R2210 T4208 T4207 pobj prostaglandin,of
R2211 T4260 T4261 compound T,cells
R2212 T4209 T4208 nummod E2,prostaglandin
R2213 T4210 T4208 cc and,prostaglandin
R2214 T4211 T4212 amod nitric,oxide
R2215 T4261 T4257 pobj cells,by
R2216 T4212 T4208 conj oxide,prostaglandin
R2217 T4213 T4194 prep by,stimulates
R2218 T4262 T4261 prep in,cells
R2219 T4214 T4213 pobj chondrocytes,by
R2220 T4215 T4194 punct ",",stimulates
R2221 T4216 T4194 cc and,stimulates
R2222 T4263 T4267 det the,]
R2223 T4217 T4194 conj has,stimulates
R2224 T4218 T4219 det the,ability
R2225 T4219 T4217 dobj ability,has
R2226 T4264 T4265 compound synovium,[
R2227 T4220 T4221 aux to,differentiate
R2228 T4221 T4219 acl differentiate,ability
R2229 T4222 T4221 cc and,differentiate
R2230 T4265 T4267 compound [,]
R2231 T4223 T4221 conj activate,differentiate
R2232 T4224 T4226 det the,cells
R2233 T4225 T4226 amod dendritic,cells
R2234 T4226 T4223 dobj cells,activate
R2235 T4227 T4229 compound [,]
R2236 T4228 T4229 compound 8-10,]
R2237 T4266 T4267 compound "11,12",]
R2238 T4229 T4226 appos ],cells
R2239 T4230 T4194 punct .,stimulates
R2240 T4231 T4237 nsubj Levels,were
R2241 T4267 T4262 pobj ],in
R2242 T4232 T4231 prep of,Levels
R2243 T4233 T4232 pobj IL-17,of
R2244 T4234 T4231 prep in,Levels
R2245 T4268 T4256 punct .,produced
R2246 T4235 T4236 amod synovial,fluids
R2247 T4236 T4234 pobj fluids,in
R2248 T4237 T4237 ROOT were,were
R2249 T4269 T4271 nsubj IL-15,secreted
R2250 T4238 T4239 advmod significantly,higher
R2251 T4270 T4269 punct ",",IL-15
R2252 T4271 T4271 ROOT secreted,secreted
R2253 T4272 T4271 prep from,secreted
R2254 T4336 T4348 mark Although,documented
R2255 T4273 T4274 amod activated,macrophages
R2256 T4337 T4338 det the,contribution
R2257 T4338 T4348 nsubjpass contribution,documented
R2258 T4274 T4272 pobj macrophages,from
R2259 T4339 T4338 prep of,contribution
R2260 T4340 T4339 pobj IL-17,of
R2261 T4341 T4338 prep in,contribution
R2262 T4275 T4278 punct ",",reported
R2263 T4342 T4343 amod joint,inflammation
R2264 T4343 T4341 pobj inflammation,in
R2265 T4276 T4278 aux has,reported
R2266 T4344 T4343 prep in,inflammation
R2267 T4345 T4344 pobj RA,in
R2268 T4346 T4348 aux has,documented
R2269 T4277 T4278 auxpass been,reported
R2270 T4347 T4348 auxpass been,documented
R2271 T4348 T4364 advcl documented,remains
R2272 T4349 T4348 prep in,documented
R2273 T4278 T4271 conj reported,secreted
R2274 T4350 T4351 amod earlier,studies
R2275 T4351 T4349 pobj studies,in
R2276 T4352 T4354 compound [,]
R2277 T4279 T4280 aux to,be
R2278 T4353 T4354 compound "12,15,16",]
R2279 T4354 T4364 nsubj ],remains
R2280 T4355 T4354 punct ",",]
R2281 T4356 T4360 det the,pathway
R2282 T4357 T4360 amod intracellular,pathway
R2283 T4358 T4360 nmod signal,pathway
R2284 T4280 T4278 xcomp be,reported
R2285 T4359 T4360 compound transduction,pathway
R2286 T4360 T4354 appos pathway,]
R2287 T4281 T4283 det an,trigger
R2288 T4361 T4360 prep for,pathway
R2289 T4282 T4283 amod important,trigger
R2290 T4362 T4363 amod IL-17,production
R2291 T4363 T4361 pobj production,for
R2292 T4283 T4280 attr trigger,be
R2293 T4364 T4364 ROOT remains,remains
R2294 T4365 T4364 acomp uncertain,remains
R2295 T4366 T4364 punct .,remains
R2296 T4284 T4283 prep of,trigger
R2297 T4367 T4367 ROOT In,In
R2298 T4368 T4370 det the,study
R2299 T4369 T4370 amod present,study
R2300 T4285 T4286 amod IL-17,production
R2301 T4370 T4367 pobj study,In
R2302 T4286 T4284 pobj production,of
R2303 T4371 T4372 nsubj we,used
R2304 T4372 T4370 relcl used,study
R2305 T4373 T4374 amod various,stimuli
R2306 T4287 T4286 prep in,production
R2307 T4374 T4372 dobj stimuli,used
R2308 T4375 T4376 aux to,investigate
R2309 T4376 T4372 xcomp investigate,used
R2310 T4377 T4378 amod IL-17,production
R2311 T4288 T4292 compound RA,cells
R2312 T4378 T4376 dobj production,investigate
R2313 T4379 T4378 prep in,production
R2314 T4289 T4290 compound peripheral,blood
R2315 T4380 T4379 pobj PBMC,in
R2316 T4381 T4380 prep of,PBMC
R2317 T4382 T4381 pobj patients,of
R2318 T4290 T4291 compound blood,mononuclear
R2319 T4383 T4382 prep with,patients
R2320 T4384 T4383 pobj RA,with
R2321 T4385 T4384 cc and,RA
R2322 T4291 T4292 compound mononuclear,cells
R2323 T4386 T4389 poss its,pathway
R2324 T4387 T4389 amod signaling,pathway
R2325 T4292 T4287 pobj cells,in
R2326 T4388 T4389 compound transduction,pathway
R2327 T4389 T4384 conj pathway,RA
R2328 T4390 T4367 punct .,In
R2329 T4293 T4286 punct (,production
R2330 T4391 T4392 nsubj We,found
R2331 T4392 T4392 ROOT found,found
R2332 T4393 T4404 mark that,/
R2333 T4394 T4397 det the,pathway
R2334 T4395 T4397 amod intracellular,pathway
R2335 T4396 T4397 amod signaling,pathway
R2336 T4294 T4283 appos PBMC,trigger
R2337 T4397 T4404 nsubj pathway,/
R2338 T4398 T4397 acl involving,pathway
R2339 T4399 T4398 dobj phosphoinositide,involving
R2340 T4400 T4403 nmod 3-kinase,)
R2341 T4295 T4283 punct ),trigger
R2342 T4401 T4402 punct (,PI3K
R2343 T4402 T4400 appos PI3K,3-kinase
R2344 T4403 T4397 punct ),pathway
R2345 T4296 T4278 agent by,reported
R2346 T4404 T4392 ccomp /,found
R2347 T4405 T4404 dobj Akt,/
R2348 T4297 T4296 pobj cyclosporine,by
R2349 T4406 T4405 cc and,Akt
R2350 T4407 T4405 conj NF-κB,Akt
R2351 T4298 T4297 cc and,cyclosporine
R2352 T4408 T4410 aux might,involved
R2353 T4409 T4410 auxpass be,involved
R2354 T4410 T4392 conj involved,found
R2355 T4411 T4410 prep in,involved
R2356 T4299 T4301 amod steroid,pathways
R2357 T4412 T4413 det the,overproduction
R2358 T4413 T4411 pobj overproduction,in
R2359 T4414 T4413 prep of,overproduction
R2360 T4300 T4301 amod sensitive,pathways
R2361 T4415 T4419 det the,IL-17
R2362 T4416 T4419 amod key,IL-17
R2363 T4301 T4297 conj pathways,cyclosporine
R2364 T4417 T4418 amod inflammatory,cytokine
R2365 T4418 T4419 compound cytokine,IL-17
R2366 T4419 T4414 pobj IL-17,of
R2367 T4420 T4419 prep in,IL-17
R2368 T4302 T4304 nmod [,]
R2369 T4421 T4420 pobj RA,in
R2370 T4422 T4392 punct .,found
R2371 T4303 T4304 nummod 13,]
R2372 T4423 T4424 det These,results
R2373 T4424 T4426 nsubj results,provide
R2374 T4425 T4426 aux might,provide
R2375 T4304 T4278 conj ],reported
R2376 T4426 T4426 ROOT provide,provide
R2377 T4427 T4428 amod new,insights
R2378 T4428 T4426 dobj insights,provide
R2379 T4305 T4278 punct .,reported
R2380 T4429 T4426 prep into,provide
R2381 T4430 T4431 det the,pathogenesis
R2382 T4431 T4429 pobj pathogenesis,into
R2383 T4432 T4431 prep of,pathogenesis
R2384 T4306 T4312 advmod Recently,showed
R2385 T4307 T4312 punct ",",showed
R2386 T4433 T4432 pobj RA,of
R2387 T4434 T4431 cc and,pathogenesis
R2388 T4435 T4436 amod future,directions
R2389 T4308 T4312 nsubj Happel,showed
R2390 T4436 T4431 conj directions,pathogenesis
R2391 T4437 T4436 prep for,directions
R2392 T4438 T4440 amod new,strategies
R2393 T4309 T4308 cc and,Happel
R2394 T4439 T4440 amod therapeutic,strategies
R2395 T4440 T4437 pobj strategies,for
R2396 T4310 T4308 conj colleagues,Happel
R2397 T4441 T4440 prep in,strategies
R2398 T4311 T4312 advmod also,showed
R2399 T4442 T4441 pobj RA,in
R2400 T4443 T4426 punct .,provide
R2401 T4312 T4312 ROOT showed,showed
R2402 T4313 T4316 mark that,be
R2403 T4314 T4316 nsubj IL-23,be
R2404 T4315 T4316 aux could,be
R2405 T4316 T4312 ccomp be,showed
R2406 T4317 T4319 det an,trigger
R2407 T4318 T4319 amod efficient,trigger
R2408 T4319 T4316 attr trigger,be
R2409 T4320 T4319 prep of,trigger
R2410 T4321 T4322 amod IL-17,production
R2412 T4322 T4320 pobj production,of
R2414 T4323 T4319 prep from,trigger
R2416 T4324 T4326 preconj both,+
R2419 T4325 T4326 nummod CD4,+
R2422 T4326 T4323 pobj +,from
R2423 T4327 T4326 cc and,+
R2424 T4328 T4331 nummod CD8,cells
R2426 T4329 T4331 nummod +,cells
R2428 T4330 T4331 compound T,cells
R2430 T4331 T4326 conj cells,+
R2436 T4332 T4334 nmod [,]
R2437 T4333 T4334 nummod 14,]
R2440 T4334 T4331 appos ],cells
R2443 T4335 T4312 punct .,showed
R2640 T5038 T5042 nsubjpass Patients,obtained
R2641 T5039 T5040 amod Informed,consent
R2642 T5040 T5042 nsubjpass consent,obtained
R2643 T5041 T5042 auxpass was,obtained
R2644 T5042 T5042 ROOT obtained,obtained
R2645 T5043 T5042 prep from,obtained
R2646 T5044 T5045 nummod 24,patients
R2647 T5045 T5043 pobj patients,from
R2648 T5046 T5045 punct (,patients
R2649 T5047 T5048 nummod 5,men
R2650 T5048 T5045 appos men,patients
R2651 T5049 T5048 cc and,men
R2652 T5050 T5051 nummod 19,women
R2653 T5051 T5048 conj women,men
R2654 T5052 T5045 punct ),patients
R2655 T5053 T5042 prep with,obtained
R2656 T5054 T5053 pobj RA,with
R2657 T5055 T5056 nsubj who,fulfilled
R2658 T5056 T5042 conj fulfilled,obtained
R2659 T5057 T5060 det the,criteria
R2660 T5058 T5060 nummod 1987,criteria
R2661 T5059 T5060 amod revised,criteria
R2662 T5060 T5056 dobj criteria,fulfilled
R2663 T5061 T5060 prep of,criteria
R2664 T5062 T5064 det the,College
R2665 T5063 T5064 compound American,College
R2666 T5064 T5061 pobj College,of
R2667 T5065 T5064 prep of,College
R2668 T5066 T5065 pobj Rheumatology,of
R2669 T5067 T5064 punct (,College
R2670 T5068 T5072 advmod formerly,Association
R2671 T5069 T5072 det the,Association
R2672 T5070 T5072 compound American,Association
R2673 T5071 T5072 compound Rheumatism,Association
R2674 T5072 T5064 appos Association,College
R2675 T5073 T5064 punct ),College
R2676 T5074 T5076 nmod [,]
R2677 T5075 T5076 nummod 17,]
R2678 T5076 T5060 appos ],criteria
R2679 T5077 T5042 punct .,obtained
R2680 T5078 T5079 det The,age
R2681 T5079 T5085 nsubj age,was
R2682 T5080 T5079 prep of,age
R2683 T5081 T5082 det the,patients
R2684 T5082 T5080 pobj patients,of
R2685 T5083 T5082 prep with,patients
R2686 T5084 T5083 pobj RA,with
R2687 T5085 T5085 ROOT was,was
R2688 T5086 T5087 nummod 50,±
R2689 T5087 T5085 attr ±,was
R2690 T5088 T5087 nummod 8,±
R2691 T5089 T5092 punct (,SEM
R2692 T5090 T5092 amod mean,SEM
R2693 T5091 T5092 compound ±,SEM
R2694 T5092 T5087 appos SEM,±
R2695 T5093 T5092 punct ),SEM
R2696 T5094 T5087 appos years,±
R2697 T5095 T5096 punct (,range
R2698 T5096 T5087 appos range,±
R2699 T5097 T5096 nummod 23,range
R2700 T5098 T5099 nummod 71,years
R2701 T5099 T5096 appos years,range
R2702 T5100 T5096 punct ),range
R2703 T5101 T5087 punct .,±
R2704 T5102 T5103 det All,medications
R2705 T5103 T5105 nsubjpass medications,stopped
R2706 T5104 T5105 auxpass were,stopped
R2707 T5105 T5105 ROOT stopped,stopped
R2708 T5106 T5107 nummod 48,hours
R2709 T5107 T5108 npadvmod hours,before
R2710 T5108 T5105 prep before,stopped
R2711 T5109 T5108 pobj entry,before
R2712 T5110 T5109 prep to,entry
R2713 T5111 T5112 det the,study
R2714 T5112 T5110 pobj study,to
R2715 T5113 T5105 punct .,stopped
R2716 T5114 T5116 nsubjpass Comparisons,made
R2717 T5115 T5116 auxpass were,made
R2718 T5116 T5116 ROOT made,made
R2719 T5117 T5116 prep with,made
R2720 T5118 T5119 nummod 14,patients
R2721 T5119 T5117 pobj patients,with
R2722 T5120 T5119 prep with,patients
R2723 T5121 T5120 pobj OA,with
R2724 T5122 T5121 punct (,OA
R2725 T5123 T5124 nummod 3,men
R2726 T5124 T5121 appos men,OA
R2727 T5125 T5124 cc and,men
R2728 T5126 T5127 nummod 11,women
R2729 T5127 T5124 conj women,men
R2730 T5128 T5121 punct ),OA
R2731 T5129 T5120 cc and,with
R2732 T5130 T5120 conj with,with
R2733 T5131 T5133 nummod 14,controls
R2734 T5132 T5133 amod healthy,controls
R2735 T5133 T5130 pobj controls,with
R2736 T5134 T5133 punct (,controls
R2737 T5135 T5136 nummod 3,men
R2738 T5136 T5133 appos men,controls
R2739 T5137 T5136 cc and,men
R2740 T5138 T5139 nummod 11,women
R2741 T5139 T5136 conj women,men
R2742 T5140 T5133 punct ),controls
R2743 T5141 T5142 nsubj who,had
R2744 T5142 T5133 relcl had,controls
R2745 T5143 T5145 det no,diseases
R2746 T5144 T5145 amod rheumatic,diseases
R2747 T5145 T5142 dobj diseases,had
R2748 T5146 T5116 punct .,made
R2749 T5147 T5149 det The,ages
R2750 T5148 T5149 amod mean,ages
R2751 T5149 T5159 nsubj ages,were
R2752 T5150 T5149 prep of,ages
R2753 T5151 T5152 det the,patients
R2754 T5152 T5150 pobj patients,of
R2755 T5153 T5152 prep with,patients
R2756 T5154 T5153 pobj OA,with
R2757 T5155 T5154 cc and,OA
R2758 T5156 T5158 det the,controls
R2759 T5157 T5158 amod healthy,controls
R2760 T5158 T5154 conj controls,OA
R2761 T5159 T5159 ROOT were,were
R2762 T5160 T5161 nummod 50,±
R2763 T5161 T5159 attr ±,were
R2764 T5162 T5163 nummod 8,years
R2765 T5163 T5161 appos years,±
R2766 T5164 T5165 punct (,range
R2767 T5165 T5163 parataxis range,years
R2768 T5166 T5165 nummod 34,range
R2769 T5167 T5168 nummod 68,years
R2770 T5168 T5165 appos years,range
R2771 T5169 T5165 punct ),range
R2772 T5170 T5163 cc and,years
R2773 T5171 T5172 nummod 30,±
R2774 T5172 T5163 conj ±,years
R2775 T5173 T5174 nummod 6,years
R2776 T5174 T5172 appos years,±
R2777 T5175 T5176 punct (,range
R2778 T5176 T5174 parataxis range,years
R2779 T5177 T5176 nummod 24,range
R2780 T5178 T5179 nummod 57,years
R2781 T5179 T5176 appos years,range
R2782 T5180 T5176 punct ),range
R2783 T5181 T5159 punct .,were
R2784 T5182 T5183 amod Informed,consent
R2785 T5183 T5185 nsubjpass consent,obtained
R2786 T5184 T5185 auxpass was,obtained
R2787 T5185 T5185 ROOT obtained,obtained
R2788 T5186 T5185 punct ",",obtained
R2789 T5187 T5185 cc and,obtained
R2790 T5188 T5189 det the,protocol
R2791 T5189 T5191 nsubjpass protocol,approved
R2792 T5190 T5191 auxpass was,approved
R2793 T5191 T5185 conj approved,obtained
R2794 T5192 T5191 agent by,approved
R2795 T5193 T5195 det the,University
R2796 T5194 T5195 compound Catholic,University
R2797 T5195 T5201 nmod University,Committee
R2798 T5196 T5195 prep of,University
R2799 T5197 T5196 pobj Korea,of
R2800 T5198 T5200 compound Human,Ethics
R2801 T5199 T5200 compound Research,Ethics
R2802 T5200 T5201 compound Ethics,Committee
R2803 T5201 T5192 pobj Committee,by
R2804 T5202 T5191 punct .,approved
R2971 T5665 T5667 compound Reagents,IL-17
R2972 T5666 T5667 compound Recombinant,IL-17
R2973 T5667 T5695 nsubjpass IL-17,purchased
R2974 T5668 T5667 punct ",",IL-17
R2975 T5669 T5667 conj IL-18,IL-17
R2976 T5670 T5669 punct ",",IL-18
R2977 T5671 T5669 conj IL-15,IL-18
R2978 T5672 T5671 punct ",",IL-15
R2979 T5673 T5675 nmod monocyte,protein-1
R2980 T5674 T5675 compound chemoattractant,protein-1
R2981 T5675 T5671 appos protein-1,IL-15
R2982 T5676 T5677 punct (,MCP-1
R2983 T5677 T5675 appos MCP-1,protein-1
R2984 T5678 T5677 punct ),MCP-1
R2985 T5679 T5671 punct ",",IL-15
R2986 T5680 T5682 nmod macrophage,protein
R2987 T5681 T5682 amod inflammatory,protein
R2988 T5682 T5667 appos protein,IL-17
R2989 T5683 T5684 punct (,MIP
R2990 T5684 T5682 appos MIP,protein
R2991 T5685 T5684 punct ),MIP
R2992 T5686 T5687 nummod -1,α
R2993 T5687 T5667 appos α,IL-17
R2994 T5688 T5687 punct ",",α
R2995 T5689 T5687 conj MIP-1β,α
R2996 T5690 T5689 punct ",",MIP-1β
R2997 T5691 T5689 conj IL-6,MIP-1β
R2998 T5692 T5691 cc and,IL-6
R2999 T5693 T5691 conj IL-8,IL-6
R3000 T5694 T5695 auxpass were,purchased
R3001 T5695 T5695 ROOT purchased,purchased
R3002 T5696 T5695 prep from,purchased
R3003 T5697 T5700 nmod R,systems
R3004 T5698 T5697 cc &,R
R3005 T5699 T5697 conj D,R
R3006 T5700 T5696 pobj systems,from
R3007 T5701 T5700 punct (,systems
R3008 T5702 T5700 appos Minneapolis,systems
R3009 T5703 T5702 punct ",",Minneapolis
R3010 T5704 T5702 conj MN,Minneapolis
R3011 T5705 T5704 punct ",",MN
R3012 T5706 T5704 npadvmod USA,MN
R3013 T5707 T5704 punct ),MN
R3014 T5708 T5695 punct .,purchased
R3015 T5709 T5712 amod Recombinant,factor
R3016 T5710 T5712 amod transforming,factor
R3017 T5711 T5712 compound growth,factor
R3018 T5712 T5719 nsubjpass factor,purchased
R3019 T5713 T5714 punct (,TGF
R3020 T5714 T5712 appos TGF,factor
R3021 T5715 T5714 punct ),TGF
R3022 T5716 T5717 punct -,β
R3023 T5717 T5712 appos β,factor
R3024 T5718 T5719 auxpass was,purchased
R3025 T5719 T5719 ROOT purchased,purchased
R3026 T5720 T5719 prep from,purchased
R3027 T5721 T5720 pobj Peprotech,from
R3028 T5722 T5721 punct (,Peprotech
R3029 T5723 T5721 appos London,Peprotech
R3030 T5724 T5723 punct ",",London
R3031 T5725 T5723 appos UK,London
R3032 T5726 T5723 punct ),London
R3033 T5727 T5719 punct .,purchased
R3034 T5728 T5729 amod Recombinant,TNF-α
R3035 T5729 T5733 nsubjpass TNF-α,purchased
R3036 T5730 T5729 cc and,TNF-α
R3037 T5731 T5729 conj IL-1,TNF-α
R3038 T5732 T5733 auxpass were,purchased
R3039 T5733 T5733 ROOT purchased,purchased
R3040 T5734 T5733 prep from,purchased
R3041 T5735 T5736 compound Endogen,Inc.
R3042 T5736 T5734 pobj Inc.,from
R3043 T5737 T5736 punct (,Inc.
R3044 T5738 T5736 npadvmod Cambridge,Inc.
R3045 T5739 T5738 punct ",",Cambridge
R3046 T5740 T5738 conj MA,Cambridge
R3047 T5741 T5740 punct ",",MA
R3048 T5742 T5740 conj USA,MA
R3049 T5743 T5740 punct ),MA
R3050 T5744 T5733 punct .,purchased
R3051 T5745 T5746 compound Cyclosporin,A
R3052 T5746 T5748 nsubjpass A,provided
R3053 T5747 T5748 auxpass was,provided
R3054 T5748 T5748 ROOT provided,provided
R3055 T5749 T5748 agent by,provided
R3056 T5750 T5751 compound Sandos,Ltd.
R3057 T5751 T5749 pobj Ltd.,by
R3058 T5752 T5751 punct (,Ltd.
R3059 T5753 T5751 npadvmod Basel,Ltd.
R3060 T5754 T5753 punct ",",Basel
R3061 T5755 T5753 appos Switzerland,Basel
R3062 T5756 T5753 punct ),Basel
R3063 T5757 T5748 punct .,provided
R3064 T5758 T5776 nsubjpass Phytohemagglutinin,obtained
R3065 T5759 T5760 punct (,PHA
R3066 T5760 T5758 appos PHA,Phytohemagglutinin
R3067 T5761 T5760 punct ),PHA
R3068 T5762 T5758 punct ",",Phytohemagglutinin
R3069 T5763 T5764 amod pyrrolidine,dithiocarbamate
R3070 T5764 T5758 appos dithiocarbamate,Phytohemagglutinin
R3071 T5765 T5766 punct (,PDTC
R3072 T5766 T5764 appos PDTC,dithiocarbamate
R3073 T5767 T5766 punct ),PDTC
R3074 T5768 T5764 punct ",",dithiocarbamate
R3075 T5769 T5764 conj rapamycin,dithiocarbamate
R3076 T5770 T5769 punct ",",rapamycin
R3077 T5771 T5769 conj dexamethasone,rapamycin
R3078 T5772 T5771 cc and,dexamethasone
R3079 T5773 T5771 conj curcumin,dexamethasone
R3080 T5774 T5776 auxpass were,obtained
R3081 T5775 T5776 dep all,obtained
R3082 T5776 T5776 ROOT obtained,obtained
R3083 T5777 T5776 prep from,obtained
R3084 T5778 T5781 det the,Co.
R3085 T5779 T5781 compound Sigma,Co.
R3086 T5780 T5781 compound Chemical,Co.
R3087 T5781 T5777 pobj Co.,from
R3088 T5782 T5781 punct (,Co.
R3089 T5783 T5784 compound St,Louis
R3090 T5784 T5781 appos Louis,Co.
R3091 T5785 T5781 punct ",",Co.
R3092 T5786 T5781 conj MA,Co.
R3093 T5787 T5786 punct ",",MA
R3094 T5788 T5786 conj USA,MA
R3095 T5789 T5776 punct ),obtained
R3096 T5790 T5776 punct .,obtained
R3097 T5791 T5793 amod Anti-CD3,antibody
R3098 T5792 T5793 amod monoclonal,antibody
R3099 T5793 T5799 nsubjpass antibody,obtained
R3100 T5794 T5793 cc and,antibody
R3101 T5795 T5797 amod anti-CD28,antibody
R3102 T5796 T5797 amod monoclonal,antibody
R3103 T5797 T5793 conj antibody,antibody
R3104 T5798 T5799 auxpass were,obtained
R3105 T5799 T5799 ROOT obtained,obtained
R3106 T5800 T5799 prep from,obtained
R3107 T5801 T5802 compound BD,Biosciences
R3108 T5802 T5800 pobj Biosciences,from
R3109 T5803 T5802 punct (,Biosciences
R3110 T5804 T5805 compound San,Diego
R3111 T5805 T5802 npadvmod Diego,Biosciences
R3112 T5806 T5805 punct ",",Diego
R3113 T5807 T5805 conj CA,Diego
R3114 T5808 T5807 punct ",",CA
R3115 T5809 T5807 conj USA,CA
R3116 T5810 T5802 punct ),Biosciences
R3117 T5811 T5799 punct .,obtained
R3118 T5812 T5822 nsubjpass LY294002,obtained
R3119 T5813 T5812 punct ",",LY294002
R3120 T5814 T5822 nsubjpass SB203580,obtained
R3121 T5815 T5814 punct ",",SB203580
R3122 T5816 T5814 conj FK506,SB203580
R3123 T5817 T5816 punct ",",FK506
R3124 T5818 T5816 conj wortmannin,FK506
R3125 T5819 T5818 cc and,wortmannin
R3126 T5820 T5822 nsubjpass PD98059,obtained
R3127 T5821 T5822 auxpass were,obtained
R3128 T5822 T5822 ROOT obtained,obtained
R3129 T5823 T5822 prep from,obtained
R3130 T5824 T5823 pobj Calbiochem,from
R3131 T5825 T5826 punct (,Schwalbach
R3132 T5826 T5824 appos Schwalbach,Calbiochem
R3133 T5827 T5826 punct ",",Schwalbach
R3134 T5828 T5826 appos Germany,Schwalbach
R3135 T5829 T5826 punct ),Schwalbach
R3136 T5830 T5822 punct .,obtained
R3328 T6294 T6308 nsubjpass Production,prepared
R3329 T6295 T6294 prep of,Production
R3330 T6296 T6295 pobj IL-17,of
R3331 T6297 T6294 prep by,Production
R3332 T6298 T6301 compound T,activation
R3333 T6299 T6300 compound cell,receptor
R3334 T6300 T6301 compound receptor,activation
R3335 T6301 T6297 pobj activation,by
R3336 T6302 T6301 punct ",",activation
R3337 T6303 T6301 conj cytokines,activation
R3338 T6304 T6303 cc or,cytokines
R3339 T6305 T6306 compound chemokines,PBMC
R3340 T6306 T6303 conj PBMC,cytokines
R3341 T6307 T6308 auxpass were,prepared
R3342 T6308 T6308 ROOT prepared,prepared
R3343 T6309 T6308 prep from,prepared
R3344 T6310 T6311 amod heparinized,blood
R3345 T6311 T6309 pobj blood,from
R3346 T6312 T6308 agent by,prepared
R3347 T6313 T6318 nmod Ficoll-Hypaque,centrifugation
R3348 T6314 T6315 punct (,SG1077
R3349 T6315 T6313 appos SG1077,Ficoll-Hypaque
R3350 T6316 T6318 punct ),centrifugation
R3351 T6317 T6318 amod density-gradient,centrifugation
R3352 T6318 T6312 pobj centrifugation,by
R3353 T6319 T6308 punct .,prepared
R3354 T6320 T6321 compound Cell,cultures
R3355 T6321 T6323 nsubjpass cultures,performed
R3356 T6322 T6323 auxpass were,performed
R3357 T6323 T6323 ROOT performed,performed
R3358 T6324 T6325 mark as,described
R3359 T6325 T6323 advcl described,performed
R3360 T6326 T6329 advmod previously,]
R3361 T6327 T6329 nmod [,]
R3362 T6328 T6329 nummod 18,]
R3363 T6329 T6325 dobj ],described
R3364 T6330 T6323 punct .,performed
R3365 T6331 T6338 prep In,adjusted
R3366 T6332 T6331 pobj brief,In
R3367 T6333 T6338 punct ",",adjusted
R3368 T6334 T6336 det the,suspensions
R3369 T6335 T6336 compound cell,suspensions
R3370 T6336 T6338 nsubjpass suspensions,adjusted
R3371 T6337 T6338 auxpass were,adjusted
R3372 T6338 T6338 ROOT adjusted,adjusted
R3373 T6339 T6348 prep to,supplemented
R3374 T6340 T6341 det a,concentration
R3375 T6341 T6339 pobj concentration,to
R3376 T6342 T6341 prep of,concentration
R3377 T6343 T6342 pobj 106/ml,of
R3378 T6344 T6341 prep in,concentration
R3379 T6345 T6348 nsubj RPMI,supplemented
R3380 T6346 T6347 nummod 1640,medium
R3381 T6347 T6348 nsubj medium,supplemented
R3382 T6348 T6338 xcomp supplemented,adjusted
R3383 T6349 T6348 prep with,supplemented
R3384 T6350 T6351 nummod 10,%
R3385 T6351 T6354 compound %,serum
R3386 T6352 T6353 amod fetal,calf
R3387 T6353 T6354 compound calf,serum
R3388 T6354 T6349 pobj serum,with
R3389 T6355 T6354 punct ",",serum
R3390 T6356 T6358 nummod 100,penicillin
R3391 T6357 T6358 compound U/ml,penicillin
R3392 T6358 T6354 conj penicillin,serum
R3393 T6359 T6358 punct ",",penicillin
R3394 T6360 T6362 nummod 100,streptomycin
R3395 T6361 T6362 compound mg/ml,streptomycin
R3396 T6362 T6358 conj streptomycin,penicillin
R3397 T6363 T6362 cc and,streptomycin
R3398 T6364 T6366 nummod 2,L-glutamine
R3399 T6365 T6366 compound mM,L-glutamine
R3400 T6366 T6362 conj L-glutamine,streptomycin
R3401 T6367 T6338 punct .,adjusted
R3402 T6368 T6369 compound Cell,suspension
R3403 T6369 T6375 nsubjpass suspension,dispensed
R3404 T6370 T6372 punct (,ml
R3405 T6371 T6372 nummod 1,ml
R3406 T6372 T6369 appos ml,suspension
R3407 T6373 T6372 punct ),ml
R3408 T6374 T6375 auxpass was,dispensed
R3409 T6375 T6375 ROOT dispensed,dispensed
R3410 T6376 T6375 prep into,dispensed
R3411 T6377 T6379 amod 24-well,plates
R3412 T6378 T6379 amod multi-well,plates
R3413 T6379 T6376 pobj plates,into
R3414 T6380 T6379 punct (,plates
R3415 T6381 T6381 ROOT Nunc,Nunc
R3416 T6382 T6381 punct ",",Nunc
R3417 T6383 T6381 conj Roskilde,Nunc
R3418 T6384 T6383 punct ",",Roskilde
R3419 T6385 T6383 npadvmod Denmark,Roskilde
R3420 T6386 T6383 punct ),Roskilde
R3421 T6387 T6381 punct ",",Nunc
R3422 T6388 T6375 cc and,dispensed
R3423 T6389 T6375 conj incubated,dispensed
R3424 T6390 T6389 prep for,incubated
R3425 T6391 T6392 nummod 24,hours
R3426 T6392 T6390 pobj hours,for
R3427 T6393 T6389 prep at,incubated
R3428 T6394 T6395 nummod 37,°
R3429 T6395 T6393 pobj °,at
R3430 T6396 T6389 conj C,incubated
R3431 T6397 T6389 prep in,incubated
R3432 T6398 T6399 nummod 5,%
R3433 T6399 T6397 pobj %,in
R3434 T6400 T6399 nummod CO2,%
R3435 T6401 T6375 punct .,dispensed
R3436 T6402 T6415 advmod Subsequently,added
R3437 T6403 T6415 punct ",",added
R3438 T6404 T6405 amod various,concentrations
R3439 T6405 T6415 nsubjpass concentrations,added
R3440 T6406 T6405 prep of,concentrations
R3441 T6407 T6408 compound cyclosporin,A
R3442 T6408 T6406 pobj A,of
R3443 T6409 T6408 punct (,A
R3444 T6410 T6408 appos 10,A
R3445 T6411 T6412 nummod 500,ng/ml
R3446 T6412 T6410 appos ng/ml,10
R3447 T6413 T6408 punct ),A
R3448 T6414 T6415 auxpass were,added
R3449 T6415 T6415 ROOT added,added
R3450 T6416 T6415 prep to,added
R3451 T6417 T6418 det the,medium
R3452 T6418 T6416 pobj medium,to
R3453 T6419 T6418 cc and,medium
R3454 T6420 T6418 conj cells,medium
R3455 T6421 T6422 auxpass were,incubated
R3456 T6422 T6422 ROOT incubated,incubated
R3457 T6423 T6422 prep for,incubated
R3458 T6424 T6425 nummod 24,hours
R3459 T6425 T6423 pobj hours,for
R3460 T6426 T6422 punct .,incubated
R3461 T6427 T6431 aux To,added
R3462 T6428 T6429 det each,well
R3463 T6429 T6431 nsubjpass well,added
R3464 T6430 T6431 auxpass was,added
R3465 T6431 T6431 ROOT added,added
R3466 T6432 T6431 dobj FK506,added
R3467 T6433 T6432 punct ",",FK506
R3468 T6434 T6432 appos rapamycin,FK506
R3469 T6435 T6434 punct ",",rapamycin
R3470 T6436 T6434 conj curcumin,rapamycin
R3471 T6437 T6436 punct ",",curcumin
R3472 T6438 T6436 conj PDTC,curcumin
R3473 T6439 T6438 punct ",",PDTC
R3474 T6440 T6438 conj LY294002,PDTC
R3475 T6441 T6440 punct ",",LY294002
R3476 T6442 T6440 conj SB203580,LY294002
R3477 T6443 T6442 punct ",",SB203580
R3478 T6444 T6442 conj PD98059,SB203580
R3479 T6445 T6444 punct ",",PD98059
R3480 T6446 T6444 conj dexamethasone,PD98059
R3481 T6447 T6446 cc or,dexamethasone
R3482 T6448 T6446 conj wortmannin,dexamethasone
R3483 T6449 T6431 punct .,added
R3484 T6450 T6464 prep After,collected
R3485 T6451 T6450 pobj incubation,After
R3486 T6452 T6451 prep for,incubation
R3487 T6453 T6454 nummod 24,hours
R3488 T6454 T6452 pobj hours,for
R3489 T6455 T6458 punct (,stated
R3490 T6456 T6458 mark unless,stated
R3491 T6457 T6458 advmod otherwise,stated
R3492 T6458 T6464 advcl stated,collected
R3493 T6459 T6458 punct ),stated
R3494 T6460 T6464 punct ",",collected
R3495 T6461 T6462 amod cell-free,media
R3496 T6462 T6464 nsubjpass media,collected
R3497 T6463 T6464 auxpass were,collected
R3498 T6464 T6464 ROOT collected,collected
R3499 T6465 T6464 cc and,collected
R3500 T6466 T6464 conj stored,collected
R3501 T6467 T6466 prep at,stored
R3502 T6468 T6469 nummod -20,°
R3503 T6469 T6470 nummod °,C
R3504 T6470 T6467 pobj C,at
R3505 T6471 T6472 mark until,assayed
R3506 T6472 T6466 advcl assayed,stored
R3507 T6473 T6464 punct .,collected
R3508 T6474 T6475 det All,cultures
R3509 T6475 T6477 nsubjpass cultures,set
R3510 T6476 T6477 auxpass were,set
R3511 T6477 T6477 ROOT set,set
R3512 T6478 T6477 prt up,set
R3513 T6479 T6477 prep in,set
R3514 T6480 T6479 pobj triplicate,in
R3515 T6481 T6477 punct ",",set
R3516 T6482 T6477 cc and,set
R3517 T6483 T6485 nsubjpass results,expressed
R3518 T6484 T6485 auxpass are,expressed
R3519 T6485 T6477 conj expressed,set
R3520 T6486 T6485 prep as,expressed
R3521 T6487 T6486 pobj means,as
R3522 T6488 T6489 compound ±,SEM
R3523 T6489 T6487 dobj SEM,means
R3524 T6490 T6485 punct .,expressed
R3721 T6978 T6981 nummod CD4,isolation
R3722 T6979 T6981 nummod +,isolation
R3723 T6980 T6981 compound T-cell,isolation
R3724 T6981 T6987 nsubjpass isolation,used
R3725 T6982 T6981 prep by,isolation
R3726 T6983 T6982 pobj MACS,by
R3727 T6984 T6985 amod Anti-CD4,microbeads
R3728 T6985 T6987 nsubjpass microbeads,used
R3729 T6986 T6987 auxpass were,used
R3730 T6987 T6987 ROOT used,used
R3731 T6988 T6990 advmod essentially,recommended
R3732 T6989 T6990 mark as,recommended
R3733 T6990 T6987 advcl recommended,used
R3734 T6991 T6990 agent by,recommended
R3735 T6992 T6993 det the,manufacturer
R3736 T6993 T6991 pobj manufacturer,by
R3737 T6994 T6993 punct (,manufacturer
R3738 T6995 T6993 appos Miltenyi,manufacturer
R3739 T6996 T6993 punct ),manufacturer
R3740 T6997 T6999 nmod [,]
R3741 T6998 T6999 nummod 19,]
R3742 T6999 T6990 dobj ],recommended
R3743 T7000 T6987 punct .,used
R3744 T7001 T7003 nsubjpass PBMC,resuspended
R3745 T7002 T7003 auxpass were,resuspended
R3746 T7003 T7003 ROOT resuspended,resuspended
R3747 T7004 T7003 prep in,resuspended
R3748 T7005 T7006 nummod 80,μl
R3749 T7006 T7004 pobj μl,in
R3750 T7007 T7006 prep of,μl
R3751 T7008 T7007 pobj FBS,of
R3752 T7009 T7003 advcl staining,resuspended
R3753 T7010 T7009 dobj buffer,staining
R3754 T7011 T7003 punct .,resuspended
R3755 T7012 T7013 amod Anti-CD4,microbeads
R3756 T7013 T7019 nsubjpass microbeads,added
R3757 T7014 T7013 punct (,microbeads
R3758 T7015 T7016 nummod 20,μl
R3759 T7016 T7013 appos μl,microbeads
R3760 T7017 T7013 punct ),microbeads
R3761 T7018 T7019 auxpass were,added
R3762 T7019 T7019 ROOT added,added
R3763 T7020 T7019 cc and,added
R3764 T7021 T7019 conj incubated,added
R3765 T7022 T7021 prep for,incubated
R3766 T7023 T7024 nummod 15,min
R3767 T7024 T7022 pobj min,for
R3768 T7025 T7024 prep at,min
R3769 T7026 T7025 pobj 6,at
R3770 T7027 T7028 nummod 12,°
R3771 T7028 T7030 compound °,Saturating
R3772 T7029 T7030 compound C.,Saturating
R3773 T7030 T7031 nummod Saturating,amounts
R3774 T7031 T7036 nsubjpass amounts,added
R3775 T7032 T7031 prep of,amounts
R3776 T7033 T7034 amod fluorochrome-conjugated,antibodies
R3777 T7034 T7032 pobj antibodies,of
R3778 T7035 T7036 auxpass were,added
R3779 T7036 T7019 conj added,added
R3780 T7037 T7036 prep for,added
R3781 T7038 T7041 det a,min
R3782 T7039 T7041 amod further,min
R3783 T7040 T7041 nummod 10,min
R3784 T7041 T7037 pobj min,for
R3785 T7042 T7036 punct .,added
R3786 T7043 T7045 nsubjpass Cells,diluted
R3787 T7044 T7045 auxpass were,diluted
R3788 T7045 T7045 ROOT diluted,diluted
R3789 T7046 T7045 prep in,diluted
R3790 T7047 T7048 nummod 2.5,ml
R3791 T7048 T7046 pobj ml,in
R3792 T7049 T7048 prep of,ml
R3793 T7050 T7049 pobj FBS,of
R3794 T7051 T7052 compound staining,buffer
R3795 T7052 T7048 conj buffer,ml
R3796 T7053 T7048 punct ",",ml
R3797 T7054 T7056 amod pelleted,resuspended
R3798 T7055 T7056 punct ",",resuspended
R3799 T7056 T7045 conj resuspended,diluted
R3800 T7057 T7056 prep in,resuspended
R3801 T7058 T7059 nummod 500,μl
R3802 T7059 T7057 pobj μl,in
R3803 T7060 T7056 cc and,resuspended
R3804 T7061 T7062 advmod magnetically,separated
R3805 T7062 T7056 conj separated,resuspended
R3806 T7063 T7062 punct ",",separated
R3807 T7064 T7065 advmod usually,on
R3808 T7065 T7062 prep on,separated
R3809 T7066 T7068 det an,magnet
R3810 T7067 T7068 compound AutoMACS,magnet
R3811 T7068 T7065 pobj magnet,on
R3812 T7069 T7068 acl fitted,magnet
R3813 T7070 T7069 prep with,fitted
R3814 T7071 T7074 det a,column
R3815 T7072 T7074 compound MACS,column
R3816 T7073 T7074 compound MS,column
R3817 T7074 T7070 pobj column,with
R3818 T7075 T7045 punct .,diluted
R3819 T7076 T7076 ROOT Flow-through,Flow-through
R3820 T7077 T7076 cc and,Flow-through
R3821 T7078 T7081 nummod two,washes
R3822 T7079 T7080 nummod 1,ml
R3823 T7080 T7081 compound ml,washes
R3824 T7081 T7083 nsubjpass washes,collected
R3825 T7082 T7083 auxpass were,collected
R3826 T7083 T7076 conj collected,Flow-through
R3827 T7084 T7083 prep as,collected
R3828 T7085 T7087 det the,fraction
R3829 T7086 T7087 amod negative,fraction
R3830 T7087 T7084 pobj fraction,as
R3831 T7088 T7083 punct .,collected
R3832 T7089 T7090 amod Enriched,cells
R3833 T7090 T7092 nsubjpass cells,collected
R3834 T7091 T7092 auxpass were,collected
R3835 T7092 T7092 ROOT collected,collected
R3836 T7093 T7092 prep in,collected
R3837 T7094 T7097 nummod two,aliquots
R3838 T7095 T7096 nummod 0.5,ml
R3839 T7096 T7097 compound ml,aliquots
R3840 T7097 T7093 pobj aliquots,in
R3841 T7098 T7097 prep from,aliquots
R3842 T7099 T7100 det the,column
R3843 T7100 T7098 pobj column,from
R3844 T7101 T7100 prep after,column
R3845 T7102 T7101 pobj removal,after
R3846 T7103 T7102 prep from,removal
R3847 T7104 T7105 det the,magnet
R3848 T7105 T7103 pobj magnet,from
R3849 T7106 T7092 punct .,collected
R3850 T7107 T7110 advmod Alternatively,stained
R3851 T7108 T7110 punct ",",stained
R3852 T7109 T7110 nsubj cells,stained
R3853 T7110 T7110 ROOT stained,stained
R3854 T7111 T7110 prep with,stained
R3855 T7112 T7111 pobj anti-CD4,with
R3856 T7113 T7115 nsubjpass phycoerythrin,washed
R3857 T7114 T7115 auxpass were,washed
R3858 T7115 T7115 ROOT washed,washed
R3859 T7116 T7115 punct ",",washed
R3860 T7117 T7118 advmod magnetically,labeled
R3861 T7118 T7115 conj labeled,washed
R3862 T7119 T7118 prep with,labeled
R3863 T7120 T7121 amod anti-phycoerythrin,microbeads
R3864 T7121 T7119 pobj microbeads,with
R3865 T7122 T7125 punct (,added
R3866 T7123 T7124 nummod 20,μl
R3867 T7124 T7125 nsubj μl,added
R3868 T7125 T7125 ROOT added,added
R3869 T7126 T7125 prep to,added
R3870 T7127 T7128 nummod 80,μl
R3871 T7128 T7126 pobj μl,to
R3872 T7129 T7128 prep of,μl
R3873 T7130 T7131 compound cell,suspension
R3874 T7131 T7129 pobj suspension,of
R3875 T7132 T7125 punct ;,added
R3876 T7133 T7134 nummod 15,min
R3877 T7134 T7134 ROOT min,min
R3878 T7135 T7134 punct ",",min
R3879 T7136 T7134 appos 6,min
R3880 T7137 T7138 nummod 12,°
R3881 T7138 T7139 compound °,C
R3882 T7139 T7134 appos C,min
R3883 T7140 T7134 punct ),min
R3884 T7141 T7134 punct ",",min
R3885 T7142 T7134 cc and,min
R3886 T7143 T7144 advmod magnetically,separated
R3887 T7144 T7134 conj separated,min
R3888 T7145 T7146 mark as,described
R3889 T7146 T7144 advcl described,separated
R3890 T7147 T7146 advmod above,described
R3891 T7148 T7125 punct .,added
R3892 T7149 T7150 det The,purity
R3893 T7150 T7154 nsubjpass purity,assessed
R3894 T7151 T7150 prep of,purity
R3895 T7152 T7151 pobj cells,of
R3896 T7153 T7154 auxpass was,assessed
R3897 T7154 T7154 ROOT assessed,assessed
R3898 T7155 T7154 agent by,assessed
R3899 T7156 T7155 pobj flow,by
R3900 T7157 T7158 amod cytometric,analysis
R3901 T7158 T7154 npadvmod analysis,assessed
R3902 T7159 T7158 prep of,analysis
R3903 T7160 T7161 amod stained,cells
R3904 T7161 T7159 pobj cells,of
R3905 T7162 T7158 prep on,analysis
R3906 T7163 T7166 det a,sorter
R3907 T7164 T7166 compound FACS,sorter
R3908 T7165 T7166 compound Vantage,sorter
R3909 T7166 T7162 pobj sorter,on
R3910 T7167 T7154 punct .,assessed
R3911 T7168 T7179 nsubj Most,had
R3912 T7169 T7173 punct (,%
R3913 T7170 T7172 amod more,97
R3914 T7171 T7172 quantmod than,97
R3915 T7172 T7173 nummod 97,%
R3916 T7173 T7168 appos %,Most
R3917 T7174 T7173 punct ),%
R3918 T7175 T7173 prep of,%
R3919 T7176 T7178 det the,cells
R3920 T7177 T7178 amod isolated,cells
R3921 T7178 T7175 pobj cells,of
R3922 T7179 T7179 ROOT had,had
R3923 T7180 T7184 det the,marker
R3924 T7181 T7182 compound CD4,T
R3925 T7182 T7184 compound T,marker
R3926 T7183 T7184 compound cell,marker
R3927 T7184 T7179 dobj marker,had
R3928 T7185 T7179 punct .,had
R4194 T7843 T7845 amod Enzyme-linked,assay
R4195 T7844 T7845 amod immunosorbent,assay
R4196 T7845 T7853 nsubjpass assay,measured
R4197 T7846 T7845 prep of,assay
R4198 T7847 T7848 compound IL-17,IL-17
R4199 T7848 T7846 pobj IL-17,of
R4200 T7849 T7845 prep in,assay
R4201 T7850 T7851 compound culture,supernatants
R4202 T7851 T7849 pobj supernatants,in
R4203 T7852 T7853 auxpass was,measured
R4204 T7853 T7853 ROOT measured,measured
R4205 T7854 T7853 agent by,measured
R4206 T7855 T7858 nmod sandwich,assay
R4207 T7856 T7858 amod enzyme-linked,assay
R4208 T7857 T7858 compound immunosorbent,assay
R4209 T7858 T7854 pobj assay,by
R4210 T7859 T7860 mark as,described
R4211 T7860 T7853 advcl described,measured
R4212 T7861 T7864 advmod previously,]
R4213 T7862 T7864 nmod [,]
R4214 T7863 T7864 nummod 20,]
R4215 T7864 T7860 dobj ],described
R4216 T7865 T7853 punct .,measured
R4217 T7866 T7876 prep In,coated
R4218 T7867 T7866 pobj brief,In
R4219 T7868 T7876 punct ",",coated
R4220 T7869 T7871 det a,plate
R4221 T7870 T7871 amod 96-well,plate
R4222 T7871 T7876 nsubjpass plate,coated
R4223 T7872 T7873 punct (,Nunc
R4224 T7873 T7871 appos Nunc,plate
R4225 T7874 T7873 punct ),Nunc
R4226 T7875 T7876 auxpass was,coated
R4227 T7876 T7876 ROOT coated,coated
R4228 T7877 T7876 prep with,coated
R4229 T7878 T7879 nummod 4,μg
R4230 T7879 T7883 amod μg,antibodies
R4231 T7880 T7883 nmod /,antibodies
R4232 T7881 T7883 amod ml,antibodies
R4233 T7882 T7883 amod monoclonal,antibodies
R4234 T7883 T7877 pobj antibodies,with
R4235 T7884 T7883 prep against,antibodies
R4236 T7885 T7884 pobj IL-17,against
R4237 T7886 T7885 punct (,IL-17
R4238 T7887 T7885 conj R,IL-17
R4239 T7888 T7887 cc &,R
R4240 T7889 T7890 compound D,Systems
R4241 T7890 T7887 conj Systems,R
R4242 T7891 T7885 punct ),IL-17
R4243 T7892 T7876 prep at,coated
R4244 T7893 T7894 nummod 4,°
R4245 T7894 T7895 nummod °,C
R4246 T7895 T7896 compound C,overnight
R4247 T7896 T7892 pobj overnight,at
R4248 T7897 T7876 punct .,coated
R4249 T7898 T7910 prep After,/
R4250 T7899 T7898 pcomp blocking,After
R4251 T7900 T7899 prep with,blocking
R4252 T7901 T7906 amod phosphate-buffered,albumin
R4253 T7902 T7903 nummod saline/1,%
R4254 T7903 T7906 compound %,albumin
R4255 T7904 T7905 amod bovine,serum
R4256 T7905 T7906 compound serum,albumin
R4257 T7906 T7900 pobj albumin,with
R4258 T7907 T7908 punct (,BSA
R4259 T7908 T7906 npadvmod BSA,albumin
R4260 T7909 T7910 punct ),/
R4261 T7910 T7910 ROOT /,/
R4262 T7911 T7912 nummod 0.05,%
R4263 T7912 T7929 nmod %,samples
R4264 T7913 T7914 nummod Tween,20
R4265 T7914 T7929 nummod 20,samples
R4266 T7915 T7914 prep for,20
R4267 T7916 T7917 nummod 2,hours
R4268 T7917 T7915 pobj hours,for
R4269 T7918 T7917 prep at,hours
R4270 T7919 T7920 compound room,temperature
R4271 T7920 T7918 pobj temperature,at
R4272 T7921 T7917 punct (,hours
R4273 T7922 T7917 nummod 22,hours
R4274 T7923 T7925 nummod 25,C
R4275 T7924 T7925 compound °,C
R4276 T7925 T7917 appos C,hours
R4277 T7926 T7914 punct ),20
R4278 T7927 T7914 punct ",",20
R4279 T7928 T7929 compound test,samples
R4280 T7929 T7910 dobj samples,/
R4281 T7930 T7929 cc and,samples
R4282 T7931 T7934 det the,IL-17
R4283 T7932 T7934 amod standard,IL-17
R4284 T7933 T7934 compound recombinant,IL-17
R4285 T7934 T7929 conj IL-17,samples
R4286 T7935 T7942 punct (,added
R4287 T7936 T7942 nsubjpass R,added
R4288 T7937 T7936 cc &,R
R4289 T7938 T7939 compound D,Systems
R4290 T7939 T7936 conj Systems,R
R4291 T7940 T7942 punct ),added
R4292 T7941 T7942 auxpass were,added
R4293 T7942 T7934 parataxis added,IL-17
R4294 T7943 T7942 prep to,added
R4295 T7944 T7946 det the,plate
R4296 T7945 T7946 amod 96-well,plate
R4297 T7946 T7943 pobj plate,to
R4298 T7947 T7942 cc and,added
R4299 T7948 T7942 conj incubated,added
R4300 T7949 T7948 prep at,incubated
R4301 T7950 T7951 compound room,temperature
R4302 T7951 T7949 pobj temperature,at
R4303 T7952 T7948 prep for,incubated
R4304 T7953 T7954 nummod 2,hours
R4305 T7954 T7952 pobj hours,for
R4306 T7955 T7942 punct .,added
R4307 T7956 T7958 nsubjpass Plates,washed
R4308 T7957 T7958 auxpass were,washed
R4309 T7958 T7958 ROOT washed,washed
R4310 T7959 T7960 nummod four,times
R4311 T7960 T7958 npadvmod times,washed
R4312 T7961 T7958 prep with,washed
R4313 T7962 T7963 compound phosphate-buffered,saline/Tween
R4314 T7963 T7961 pobj saline/Tween,with
R4315 T7964 T7963 nummod 20,saline/Tween
R4316 T7965 T7958 punct ",",washed
R4317 T7966 T7958 cc and,washed
R4318 T7967 T7968 advmod then,incubated
R4319 T7968 T7958 conj incubated,washed
R4320 T7969 T7968 prep with,incubated
R4321 T7970 T7975 nummod 500,antibodies
R4322 T7971 T7975 amod ng/ml,antibodies
R4323 T7972 T7975 amod biotinylated,antibodies
R4324 T7973 T7975 compound mouse,antibodies
R4325 T7974 T7975 compound monoclonal,antibodies
R4326 T7975 T7969 pobj antibodies,with
R4327 T7976 T7975 prep against,antibodies
R4328 T7977 T7976 pobj IL-17,against
R4329 T7978 T7977 punct (,IL-17
R4330 T7979 T7977 conj R,IL-17
R4331 T7980 T7979 cc &,R
R4332 T7981 T7982 compound D,Systems
R4333 T7982 T7979 conj Systems,R
R4334 T7983 T7968 punct ),incubated
R4335 T7984 T7968 prep for,incubated
R4336 T7985 T7986 nummod 2,hours
R4337 T7986 T7984 pobj hours,for
R4338 T7987 T7986 prep at,hours
R4339 T7988 T7989 compound room,temperature
R4340 T7989 T7987 pobj temperature,at
R4341 T7990 T7958 punct .,washed
R4342 T7991 T8004 prep After,incubated
R4343 T7992 T7991 pcomp washing,After
R4344 T7993 T8004 punct ",",incubated
R4345 T7994 T8004 nsubjpass streptavidin,incubated
R4346 T7995 T7996 amod alkaline,phosphate
R4347 T7996 T8003 nsubj phosphate,was
R4348 T7997 T7999 amod horseradish,conjugate
R4349 T7998 T7999 amod peroxidase,conjugate
R4350 T7999 T7996 appos conjugate,phosphate
R4351 T8000 T8001 punct (,Sigma
R4352 T8001 T7999 appos Sigma,conjugate
R4353 T8002 T7999 punct ),conjugate
R4354 T8003 T8004 auxpass was,incubated
R4355 T8004 T8004 ROOT incubated,incubated
R4356 T8005 T8004 prep for,incubated
R4357 T8006 T8007 nummod 2,hours
R4358 T8007 T8005 pobj hours,for
R4359 T8008 T8010 punct ",",washed
R4360 T8009 T8010 advmod then,washed
R4361 T8010 T8004 dep washed,incubated
R4362 T8011 T8010 advmod again,washed
R4363 T8012 T8010 cc and,washed
R4364 T8013 T8010 conj incubated,washed
R4365 T8014 T8013 prep with,incubated
R4366 T8015 T8016 nummod 1,mg/ml
R4367 T8016 T8018 nmod mg/ml,phosphate
R4368 T8017 T8018 compound p-nitrophenyl,phosphate
R4369 T8018 T8014 pobj phosphate,with
R4370 T8019 T8020 punct (,Sigma
R4371 T8020 T8018 appos Sigma,phosphate
R4372 T8021 T8013 punct ),incubated
R4373 T8022 T8010 conj dissolved,washed
R4374 T8023 T8022 prep in,dissolved
R4375 T8024 T8023 pobj diethanolamine,in
R4376 T8025 T8026 punct (,Sigma
R4377 T8026 T8024 appos Sigma,diethanolamine
R4378 T8027 T8024 punct ),diethanolamine
R4379 T8028 T8029 aux to,develop
R4380 T8029 T8022 advcl develop,dissolved
R4381 T8030 T8032 det the,reaction
R4382 T8031 T8032 compound color,reaction
R4383 T8032 T8029 dobj reaction,develop
R4384 T8033 T8004 punct .,incubated
R4385 T8034 T8035 det The,reaction
R4386 T8035 T8037 nsubjpass reaction,stopped
R4387 T8036 T8037 auxpass was,stopped
R4388 T8037 T8037 ROOT stopped,stopped
R4389 T8038 T8037 agent by,stopped
R4390 T8039 T8040 det the,addition
R4391 T8040 T8038 pobj addition,by
R4392 T8041 T8040 prep of,addition
R4393 T8042 T8044 nummod 1,NaOH
R4394 T8043 T8044 compound M,NaOH
R4395 T8044 T8041 pobj NaOH,of
R4396 T8045 T8044 cc and,NaOH
R4397 T8046 T8048 det the,density
R4398 T8047 T8048 amod optical,density
R4399 T8048 T8044 conj density,NaOH
R4400 T8049 T8048 prep of,density
R4401 T8050 T8051 det each,well
R4402 T8051 T8049 pobj well,of
R4403 T8052 T8053 auxpass was,read
R4404 T8053 T8037 conj read,stopped
R4405 T8054 T8053 prep at,read
R4406 T8055 T8056 nummod 405,nm
R4407 T8056 T8054 pobj nm,at
R4408 T8057 T8053 punct .,read
R4409 T8058 T8060 det The,limit
R4410 T8059 T8060 amod lower,limit
R4411 T8060 T8064 nsubj limit,was
R4412 T8061 T8060 prep of,limit
R4413 T8062 T8063 compound IL-17,detection
R4414 T8063 T8061 pobj detection,of
R4415 T8064 T8064 ROOT was,was
R4416 T8065 T8066 nummod 10,pg/ml
R4417 T8066 T8064 attr pg/ml,was
R4418 T8067 T8064 punct .,was
R4419 T8068 T8070 amod Recombinant,IL-17
R4420 T8069 T8070 amod human,IL-17
R4421 T8070 T8071 nsubj IL-17,diluted
R4422 T8071 T8076 nsubjpass diluted,used
R4423 T8072 T8071 prep in,diluted
R4424 T8073 T8074 compound culture,medium
R4425 T8074 T8072 pobj medium,in
R4426 T8075 T8076 auxpass was,used
R4427 T8076 T8076 ROOT used,used
R4428 T8077 T8076 prep as,used
R4429 T8078 T8080 det a,standard
R4430 T8079 T8080 compound calibration,standard
R4431 T8080 T8077 pobj standard,as
R4432 T8081 T8076 punct ",",used
R4433 T8082 T8076 advcl ranging,used
R4434 T8083 T8082 prep from,ranging
R4435 T8084 T8083 pobj 10,from
R4436 T8085 T8083 prep to,from
R4437 T8086 T8087 det 2000,pg/ml
R4438 T8087 T8085 pobj pg/ml,to
R4439 T8088 T8076 punct .,used
R4440 T8089 T8091 det A,curve
R4441 T8090 T8091 amod standard,curve
R4442 T8091 T8093 nsubjpass curve,drawn
R4443 T8092 T8093 auxpass was,drawn
R4444 T8093 T8093 ROOT drawn,drawn
R4445 T8094 T8093 agent by,drawn
R4446 T8095 T8094 pcomp plotting,by
R4447 T8096 T8097 amod optical,density
R4448 T8097 T8095 dobj density,plotting
R4449 T8098 T8097 prep against,density
R4450 T8099 T8100 det the,log
R4451 T8100 T8098 pobj log,against
R4452 T8101 T8100 prep of,log
R4453 T8102 T8103 det the,concentration
R4454 T8103 T8101 pobj concentration,of
R4455 T8104 T8103 prep of,concentration
R4456 T8105 T8106 amod recombinant,cytokines
R4457 T8106 T8104 pobj cytokines,of
R4458 T8107 T8093 punct ",",drawn
R4459 T8108 T8093 cc and,drawn
R4460 T8109 T8093 conj used,drawn
R4461 T8110 T8109 prep for,used
R4462 T8111 T8110 pobj determination,for
R4463 T8112 T8111 prep of,determination
R4464 T8113 T8112 pobj IL-17,of
R4465 T8114 T8111 prep in,determination
R4466 T8115 T8116 compound test,samples
R4467 T8116 T8114 pobj samples,in
R4468 T8117 T8093 punct .,drawn
R4487 T8766 T8779 nsubjpass Quantification,incubated
R4488 T8767 T8766 prep of,Quantification
R4489 T8768 T8769 compound IL-17,mRNA
R4490 T8769 T8767 pobj mRNA,of
R4491 T8770 T8766 prep by,Quantification
R4492 T8771 T8773 amod semiquantitative,transcription
R4493 T8772 T8773 compound reverse,transcription
R4494 T8773 T8770 pobj transcription,by
R4495 T8774 T8776 compound polymerase,reaction
R4496 T8775 T8776 compound chain,reaction
R4497 T8776 T8777 compound reaction,PBMC
R4498 T8777 T8766 conj PBMC,Quantification
R4499 T8778 T8779 auxpass were,incubated
R4500 T8779 T8779 ROOT incubated,incubated
R4501 T8780 T8779 prep with,incubated
R4502 T8781 T8782 amod various,concentrations
R4503 T8782 T8780 pobj concentrations,with
R4504 T8783 T8782 prep of,concentrations
R4505 T8784 T8783 pobj anti-CD3,of
R4506 T8785 T8782 prep in,concentrations
R4507 T8786 T8787 det the,presence
R4508 T8787 T8785 pobj presence,in
R4509 T8788 T8787 cc or,presence
R4510 T8789 T8787 conj absence,presence
R4511 T8790 T8789 prep of,absence
R4512 T8791 T8790 pobj inhibitors,of
R4513 T8792 T8791 punct (,inhibitors
R4514 T8793 T8791 appos LY294002,inhibitors
R4515 T8794 T8791 punct ",",inhibitors
R4516 T8795 T8791 appos PDTC,inhibitors
R4517 T8796 T8791 punct ),inhibitors
R4518 T8797 T8779 punct .,incubated
R4519 T8798 T8806 prep After,extracted
R4520 T8799 T8800 nummod 16,hours
R4521 T8800 T8798 pobj hours,After
R4522 T8801 T8800 prep of,hours
R4523 T8802 T8801 pobj incubation,of
R4524 T8803 T8806 punct ",",extracted
R4525 T8804 T8806 nsubjpass mRNA,extracted
R4526 T8805 T8806 auxpass was,extracted
R4527 T8806 T8806 ROOT extracted,extracted
R4528 T8807 T8806 prep with,extracted
R4529 T8808 T8809 compound RNAzol,B
R4530 T8809 T8807 pobj B,with
R4531 T8810 T8812 punct (,Laboratories
R4532 T8811 T8812 compound Biotex,Laboratories
R4533 T8812 T8809 appos Laboratories,B
R4534 T8813 T8812 punct ",",Laboratories
R4535 T8814 T8812 conj Houston,Laboratories
R4536 T8815 T8814 punct ",",Houston
R4537 T8816 T8814 conj TX,Houston
R4538 T8817 T8816 punct ",",TX
R4539 T8818 T8816 conj USA,TX
R4540 T8819 T8812 punct ),Laboratories
R4541 T8820 T8806 prep in,extracted
R4542 T8821 T8820 pobj accordance,in
R4543 T8822 T8821 prep with,accordance
R4544 T8823 T8824 det the,manufacturer
R4545 T8824 T8826 poss manufacturer,instructions
R4546 T8825 T8824 case 's,manufacturer
R4547 T8826 T8822 pobj instructions,with
R4548 T8827 T8806 punct .,extracted
R4549 T8828 T8828 ROOT Reverse,Reverse
R4550 T8829 T8837 nsubjpass transcription,performed
R4551 T8830 T8829 prep of,transcription
R4552 T8831 T8832 nummod 2,μg
R4553 T8832 T8830 pobj μg,of
R4554 T8833 T8832 prep of,μg
R4555 T8834 T8835 amod total,mRNA
R4556 T8835 T8833 pobj mRNA,of
R4557 T8836 T8837 auxpass was,performed
R4558 T8837 T8828 ccomp performed,Reverse
R4559 T8838 T8837 prep at,performed
R4560 T8839 T8840 nummod 42,°
R4561 T8840 T8841 nummod °,C
R4562 T8841 T8838 pobj C,at
R4563 T8842 T8837 advcl using,performed
R4564 T8843 T8848 det the,system
R4565 T8844 T8848 nmod Superscript,system
R4566 T8845 T8848 compound ™,system
R4567 T8846 T8848 compound reverse,system
R4568 T8847 T8848 compound transcription,system
R4569 T8848 T8842 dobj system,using
R4570 T8849 T8848 punct (,system
R4571 T8850 T8848 appos Takara,system
R4572 T8851 T8850 punct ",",Takara
R4573 T8852 T8850 conj Shiga,Takara
R4574 T8853 T8852 punct ",",Shiga
R4575 T8854 T8852 npadvmod Japan,Shiga
R4576 T8855 T8852 punct ),Shiga
R4577 T8856 T8828 punct .,Reverse
R4578 T8857 T8858 compound PCR,amplification
R4579 T8858 T8863 nsubjpass amplification,performed
R4580 T8859 T8858 prep of,amplification
R4581 T8860 T8861 compound cDNA,aliquots
R4582 T8861 T8859 pobj aliquots,of
R4583 T8862 T8863 auxpass was,performed
R4584 T8863 T8863 ROOT performed,performed
R4585 T8864 T8863 prep by,performed
R4586 T8865 T8864 pcomp adding,by
R4587 T8866 T8868 nummod 2.5,dNTPs
R4588 T8867 T8868 compound mM,dNTPs
R4589 T8868 T8865 dobj dNTPs,adding
R4590 T8869 T8871 punct ",",U
R4591 T8870 T8871 nummod 2.5,U
R4592 T8871 T8868 appos U,dNTPs
R4593 T8872 T8871 prep of,U
R4594 T8873 T8875 compound Taq,polymerase
R4595 T8874 T8875 compound DNA,polymerase
R4596 T8875 T8872 pobj polymerase,of
R4597 T8876 T8877 punct (,Takara
R4598 T8877 T8875 appos Takara,polymerase
R4599 T8878 T8871 punct ),U
R4600 T8879 T8871 cc and,U
R4601 T8880 T8881 nummod 0.25,μM
R4602 T8881 T8871 conj μM,U
R4603 T8882 T8881 prep of,μM
R4604 T8883 T8882 pobj sense,of
R4605 T8884 T8883 cc and,sense
R4606 T8885 T8886 amod antisense,primers
R4607 T8886 T8883 conj primers,sense
R4608 T8887 T8863 punct .,performed
R4609 T8888 T8889 det The,reaction
R4610 T8889 T8891 nsubjpass reaction,performed
R4611 T8890 T8891 auxpass was,performed
R4612 T8891 T8891 ROOT performed,performed
R4613 T8892 T8891 prep in,performed
R4614 T8893 T8894 compound PCR,buffer
R4615 T8894 T8892 pobj buffer,in
R4616 T8895 T8898 punct (,MgCl2
R4617 T8896 T8898 nummod 1.5,MgCl2
R4618 T8897 T8898 compound mM,MgCl2
R4619 T8898 T8894 appos MgCl2,buffer
R4620 T8899 T8898 punct ",",MgCl2
R4621 T8900 T8902 nummod 50,KCl
R4622 T8901 T8902 compound mM,KCl
R4623 T8902 T8898 appos KCl,MgCl2
R4624 T8903 T8902 punct ",",KCl
R4625 T8904 T8906 nummod 10,Tris-HCl
R4626 T8905 T8906 compound mM,Tris-HCl
R4627 T8906 T8902 conj Tris-HCl,KCl
R4628 T8907 T8906 punct ",",Tris-HCl
R4629 T8908 T8906 appos pH,Tris-HCl
R4630 T8909 T8908 nummod 8.3,pH
R4631 T8910 T8906 punct ),Tris-HCl
R4632 T8911 T8891 prep in,performed
R4633 T8912 T8914 det a,volume
R4634 T8913 T8914 amod total,volume
R4635 T8914 T8911 pobj volume,in
R4636 T8915 T8914 prep of,volume
R4637 T8916 T8917 nummod 25,μl
R4638 T8917 T8915 pobj μl,of
R4639 T8918 T8891 punct .,performed
R4640 T8919 T8921 det The,sense
R4641 T8920 T8921 amod following,sense
R4642 T8921 T8929 nsubjpass sense,used
R4643 T8922 T8921 cc and,sense
R4644 T8923 T8924 amod antisense,primers
R4645 T8924 T8921 conj primers,sense
R4646 T8925 T8924 prep for,primers
R4647 T8926 T8927 det each,molecules
R4648 T8927 T8925 pobj molecules,for
R4649 T8928 T8929 auxpass were,used
R4650 T8929 T8929 ROOT used,used
R4651 T8930 T8932 punct :,sense
R4652 T8931 T8932 amod IL-17,sense
R4653 T8932 T8929 dobj sense,used
R4654 T8933 T8932 punct ",",sense
R4655 T8934 T8944 nummod 5,TTG-3
R4656 T8935 T8937 punct ',ATG
R4657 T8936 T8937 punct -,ATG
R4658 T8937 T8944 compound ATG,TTG-3
R4659 T8938 T8944 compound ACT,TTG-3
R4660 T8939 T8941 compound CCT,AAG
R4661 T8940 T8941 compound GGG,AAG
R4662 T8941 T8944 compound AAG,TTG-3
R4663 T8942 T8944 compound ACC,TTG-3
R4664 T8943 T8944 compound TCA,TTG-3
R4665 T8944 T8932 appos TTG-3,sense
R4666 T8945 T8944 case ',TTG-3
R4667 T8946 T8932 punct ;,sense
R4668 T8947 T8948 compound IL-17,antisense
R4669 T8948 T8932 conj antisense,sense
R4670 T8949 T8948 punct ",",antisense
R4671 T8950 T8948 appos 5,antisense
R4672 T8951 T8950 punct ',5
R4673 T8952 T8954 punct -,GGC
R4674 T8953 T8954 compound TTA,GGC
R4675 T8954 T8960 compound GGC,CGG-3
R4676 T8955 T8960 compound CAC,CGG-3
R4677 T8956 T8960 compound ATG,CGG-3
R4678 T8957 T8960 compound GTG,CGG-3
R4679 T8958 T8960 compound GAC,CGG-3
R4680 T8959 T8960 compound AAT,CGG-3
R4681 T8960 T8948 appos CGG-3,antisense
R4682 T8961 T8948 punct ',antisense
R4683 T8962 T8948 punct ;,antisense
R4684 T8963 T8964 amod glyceraldehyde-3-phosphate,dehydrogenase
R4685 T8964 T8948 appos dehydrogenase,antisense
R4686 T8965 T8966 punct (,GAPDH
R4687 T8966 T8964 appos GAPDH,dehydrogenase
R4688 T8967 T8966 punct ),GAPDH
R4689 T8968 T8948 conj sense,antisense
R4690 T8969 T8948 punct ",",antisense
R4691 T8970 T8948 appos 5,antisense
R4692 T8971 T8970 punct ',5
R4693 T8972 T8979 punct -,C-3
R4694 T8973 T8979 compound CGA,C-3
R4695 T8974 T8979 compound TGC,C-3
R4696 T8975 T8979 compound TGG,C-3
R4697 T8976 T8979 compound GCG,C-3
R4698 T8977 T8979 compound TGA,C-3
R4699 T8978 T8979 compound GTA,C-3
R4700 T8979 T8948 appos C-3,antisense
R4701 T8980 T8979 case ',C-3
R4702 T8981 T8948 punct ;,antisense
R4703 T8982 T8983 compound GAPDH,antisense
R4704 T8983 T8948 appos antisense,antisense
R4705 T8984 T8983 punct ",",antisense
R4706 T8985 T8994 nummod 5,CC-3
R4707 T8986 T8994 punct ',CC-3
R4708 T8987 T8994 punct -,CC-3
R4709 T8988 T8994 compound CGT,CC-3
R4710 T8989 T8994 compound TCA,CC-3
R4711 T8990 T8994 compound GCT,CC-3
R4712 T8991 T8994 compound CAG,CC-3
R4713 T8992 T8994 compound GGA,CC-3
R4714 T8993 T8994 compound TGA,CC-3
R4715 T8994 T8948 appos CC-3,antisense
R4716 T8995 T8994 punct ',CC-3
R4717 T8996 T8929 punct .,used
R4718 T8997 T8999 nsubjpass Reactions,processed
R4719 T8998 T8999 auxpass were,processed
R4720 T8999 T9066 ccomp processed,determined
R4721 T9000 T8999 prep in,processed
R4722 T9001 T9004 det a,cycler
R4723 T9002 T9004 nmod DNA,cycler
R4724 T9003 T9004 amod thermal,cycler
R4725 T9004 T9000 pobj cycler,in
R4726 T9005 T9007 punct (,Cetus
R4727 T9006 T9007 compound Perkin-Elmer,Cetus
R4728 T9007 T9004 appos Cetus,cycler
R4729 T9008 T9007 punct ",",Cetus
R4730 T9009 T9007 npadvmod Norwalk,Cetus
R4731 T9010 T9009 punct ",",Norwalk
R4732 T9011 T9009 conj CT,Norwalk
R4733 T9012 T9011 punct ",",CT
R4734 T9013 T9011 conj USA,CT
R4735 T9014 T9007 punct ),Cetus
R4736 T9015 T8999 prep through,processed
R4737 T9016 T9015 pobj cycles,through
R4738 T9017 T8999 prep for,processed
R4739 T9018 T9017 pobj 30,for
R4740 T9019 T8999 dep s,processed
R4741 T9020 T9019 prep of,s
R4742 T9021 T9020 pobj denaturation,of
R4743 T9022 T8999 prep at,processed
R4744 T9023 T9024 nummod 94,°
R4745 T9024 T9025 nummod °,C
R4746 T9025 T9022 pobj C,at
R4747 T9026 T9025 punct ",",C
R4748 T9027 T9028 nummod 1,min
R4749 T9028 T9040 nsubj min,followed
R4750 T9029 T9028 prep of,min
R4751 T9030 T9029 pcomp annealing,of
R4752 T9031 T9030 prep at,annealing
R4753 T9032 T9034 nummod 56,C
R4754 T9033 T9034 nummod °,C
R4755 T9034 T9031 pobj C,at
R4756 T9035 T9034 prep for,C
R4757 T9036 T9035 pobj GAPDH,for
R4758 T9037 T9036 cc and,GAPDH
R4759 T9038 T9036 conj IL-17,GAPDH
R4760 T9039 T9034 punct ",",C
R4761 T9040 T8999 advcl followed,processed
R4762 T9041 T9040 prep by,followed
R4763 T9042 T9043 nummod 1,min
R4764 T9043 T9041 pobj min,by
R4765 T9044 T9043 prep of,min
R4766 T9045 T9044 pobj elongation,of
R4767 T9046 T9043 prep at,min
R4768 T9047 T9048 nummod 72,°
R4769 T9048 T9051 nummod °,rounds
R4770 T9049 T9050 compound C.,PCR
R4771 T9050 T9051 compound PCR,rounds
R4772 T9051 T9046 pobj rounds,at
R4773 T9052 T9053 auxpass were,repeated
R4774 T9053 T8999 conj repeated,processed
R4775 T9054 T9053 prep for,repeated
R4776 T9055 T9056 nummod 25,cycles
R4777 T9056 T9054 pobj cycles,for
R4778 T9057 T9063 acomp each,;
R4779 T9058 T9057 prep for,each
R4780 T9059 T9060 det both,GAPDH
R4781 T9060 T9058 pobj GAPDH,for
R4782 T9061 T9060 cc and,GAPDH
R4783 T9062 T9060 conj IL-17,GAPDH
R4784 T9063 T9066 punct ;,determined
R4785 T9064 T9066 nsubjpass this,determined
R4786 T9065 T9066 auxpass was,determined
R4787 T9066 T9066 ROOT determined,determined
R4788 T9067 T9066 prep as,determined
R4789 T9068 T9067 pcomp falling,as
R4790 T9069 T9068 prep within,falling
R4791 T9070 T9072 det the,phase
R4792 T9071 T9072 amod exponential,phase
R4793 T9072 T9069 pobj phase,within
R4794 T9073 T9072 prep of,phase
R4795 T9074 T9073 pobj amplification,of
R4796 T9075 T9068 prep for,falling
R4797 T9076 T9077 det each,molecule
R4798 T9077 T9075 pobj molecule,for
R4799 T9078 T9066 punct .,determined
R4800 T9079 T9080 det The,level
R4801 T9080 T9085 nsubjpass level,presented
R4802 T9081 T9080 prep of,level
R4803 T9082 T9083 compound mRNA,expression
R4804 T9083 T9081 pobj expression,of
R4805 T9084 T9085 auxpass was,presented
R4806 T9085 T9085 ROOT presented,presented
R4807 T9086 T9085 prep as,presented
R4808 T9087 T9088 det a,ratio
R4809 T9088 T9086 pobj ratio,as
R4810 T9089 T9088 prep of,ratio
R4811 T9090 T9092 compound IL-17,product
R4812 T9091 T9092 compound PCR,product
R4813 T9092 T9089 pobj product,of
R4814 T9093 T9088 prep over,ratio
R4815 T9094 T9095 compound GAPDH,product
R4816 T9095 T9093 pobj product,over
R4817 T9096 T9085 punct .,presented
R5079 T9686 T9688 amod Western,analysis
R5080 T9687 T9688 compound blot,analysis
R5081 T9688 T9692 nsubj analysis,phosphorylated
R5082 T9689 T9688 prep of,analysis
R5083 T9690 T9689 pobj Akt,of
R5084 T9691 T9688 punct ",",analysis
R5085 T9692 T9692 ROOT phosphorylated,phosphorylated
R5086 T9693 T9692 dobj Akt,phosphorylated
R5087 T9694 T9693 cc and,Akt
R5088 T9695 T9696 compound IκB-α,PBMC
R5089 T9696 T9693 conj PBMC,Akt
R5090 T9697 T9698 auxpass were,incubated
R5091 T9698 T9692 conj incubated,phosphorylated
R5092 T9699 T9698 prep with,incubated
R5093 T9700 T9699 pobj anti-CD3,with
R5094 T9701 T9705 punct (,ml
R5095 T9702 T9703 nummod 10,μg
R5096 T9703 T9705 nmod μg,ml
R5097 T9704 T9705 compound /,ml
R5098 T9705 T9700 appos ml,anti-CD3
R5099 T9706 T9705 punct ),ml
R5100 T9707 T9698 prep in,incubated
R5101 T9708 T9709 det the,presence
R5102 T9709 T9707 pobj presence,in
R5103 T9710 T9709 cc or,presence
R5104 T9711 T9709 conj absence,presence
R5105 T9712 T9711 prep of,absence
R5106 T9713 T9712 pobj LY294002,of
R5107 T9714 T9716 punct (,μM
R5108 T9715 T9716 nummod 20,μM
R5109 T9716 T9713 appos μM,LY294002
R5110 T9717 T9716 punct ),μM
R5111 T9718 T9692 punct .,phosphorylated
R5112 T9719 T9729 prep After,prepared
R5113 T9720 T9719 pobj incubation,After
R5114 T9721 T9729 prep for,prepared
R5115 T9722 T9723 nummod 1,hour
R5116 T9723 T9721 pobj hour,for
R5117 T9724 T9729 punct ",",prepared
R5118 T9725 T9727 amod whole,lysates
R5119 T9726 T9727 compound cell,lysates
R5120 T9727 T9729 nsubjpass lysates,prepared
R5121 T9728 T9729 auxpass were,prepared
R5122 T9729 T9729 ROOT prepared,prepared
R5123 T9730 T9729 prep from,prepared
R5124 T9731 T9733 nmod about,cells
R5125 T9732 T9733 nummod 107,cells
R5126 T9733 T9730 pobj cells,from
R5127 T9734 T9733 prep by,cells
R5128 T9735 T9734 pobj homogenization,by
R5129 T9736 T9733 prep in,cells
R5130 T9737 T9739 det the,buffer
R5131 T9738 T9739 compound lysis,buffer
R5132 T9739 T9736 pobj buffer,in
R5133 T9740 T9729 punct ",",prepared
R5134 T9741 T9729 cc and,prepared
R5135 T9742 T9729 conj centrifuged,prepared
R5136 T9743 T9742 prep at,centrifuged
R5137 T9744 T9745 nummod "14,000",r.p.m.
R5138 T9745 T9743 pobj r.p.m.,at
R5139 T9746 T9745 punct (,r.p.m.
R5140 T9747 T9748 nummod "19,000",g
R5141 T9748 T9745 appos g,r.p.m.
R5142 T9749 T9745 punct ),r.p.m.
R5143 T9750 T9742 prep for,centrifuged
R5144 T9751 T9752 nummod 15,min
R5145 T9752 T9750 pobj min,for
R5146 T9753 T9729 punct .,prepared
R5147 T9754 T9755 compound Protein,concentrations
R5148 T9755 T9760 nsubjpass concentrations,determined
R5149 T9756 T9755 prep in,concentrations
R5150 T9757 T9758 det the,supernatants
R5151 T9758 T9756 pobj supernatants,in
R5152 T9759 T9760 auxpass were,determined
R5153 T9760 T9760 ROOT determined,determined
R5154 T9761 T9760 prep with,determined
R5155 T9762 T9764 det the,method
R5156 T9763 T9764 compound Bradford,method
R5157 T9764 T9761 pobj method,with
R5158 T9765 T9766 punct (,Bio-Rad
R5159 T9766 T9764 appos Bio-Rad,method
R5160 T9767 T9766 punct ",",Bio-Rad
R5161 T9768 T9766 conj Hercules,Bio-Rad
R5162 T9769 T9768 punct ",",Hercules
R5163 T9770 T9768 conj CA,Hercules
R5164 T9771 T9770 punct ",",CA
R5165 T9772 T9770 conj USA,CA
R5166 T9773 T9766 punct ),Bio-Rad
R5167 T9774 T9760 punct .,determined
R5168 T9775 T9776 compound Protein,samples
R5169 T9776 T9778 nsubjpass samples,separated
R5170 T9777 T9778 auxpass were,separated
R5171 T9778 T9778 ROOT separated,separated
R5172 T9779 T9778 prep by,separated
R5173 T9780 T9781 nummod 10,%
R5174 T9781 T9779 pobj %,by
R5175 T9782 T9783 compound SDS,PAGE
R5176 T9783 T9778 npadvmod PAGE,separated
R5177 T9784 T9778 cc and,separated
R5178 T9785 T9778 conj transferred,separated
R5179 T9786 T9785 prep to,transferred
R5180 T9787 T9789 det a,membrane
R5181 T9788 T9789 amod nitrocellulose,membrane
R5182 T9789 T9786 pobj membrane,to
R5183 T9790 T9793 punct (,Biotech
R5184 T9791 T9793 compound Amersham,Biotech
R5185 T9792 T9793 compound Pharmacia,Biotech
R5186 T9793 T9789 appos Biotech,membrane
R5187 T9794 T9793 punct ",",Biotech
R5188 T9795 T9793 conj Uppsala,Biotech
R5189 T9796 T9795 punct ",",Uppsala
R5190 T9797 T9795 conj Sweden,Uppsala
R5191 T9798 T9793 punct ),Biotech
R5192 T9799 T9778 punct .,separated
R5193 T9800 T9806 prep For,preincubated
R5194 T9801 T9802 amod western,hybridization
R5195 T9802 T9800 pobj hybridization,For
R5196 T9803 T9806 punct ",",preincubated
R5197 T9804 T9806 nsubjpass membrane,preincubated
R5198 T9805 T9806 auxpass was,preincubated
R5199 T9806 T9806 ROOT preincubated,preincubated
R5200 T9807 T9806 prep with,preincubated
R5201 T9808 T9809 nummod 0.1,%
R5202 T9809 T9810 nsubj %,skimmed
R5203 T9810 T9806 conj skimmed,preincubated
R5204 T9811 T9810 dobj milk,skimmed
R5205 T9812 T9810 prep in,skimmed
R5206 T9813 T9814 compound TBS-T,buffer
R5207 T9814 T9812 pobj buffer,in
R5208 T9815 T9817 punct (,%
R5209 T9816 T9817 nummod 0.1,%
R5210 T9817 T9837 nsubj %,phosphorylated
R5211 T9818 T9819 nummod Tween,20
R5212 T9819 T9837 dep 20,phosphorylated
R5213 T9820 T9819 prep in,20
R5214 T9821 T9822 amod Tris-buffered,saline
R5215 T9822 T9820 pobj saline,in
R5216 T9823 T9819 punct ),20
R5217 T9824 T9819 prep at,20
R5218 T9825 T9826 compound room,temperature
R5219 T9826 T9824 pobj temperature,at
R5220 T9827 T9819 prep for,20
R5221 T9828 T9829 nummod 2,hours
R5222 T9829 T9827 pobj hours,for
R5223 T9830 T9819 punct ",",20
R5224 T9831 T9833 advmod then,antibodies
R5225 T9832 T9833 amod primary,antibodies
R5226 T9833 T9819 appos antibodies,20
R5227 T9834 T9833 prep against,antibodies
R5228 T9835 T9834 pobj Akt,against
R5229 T9836 T9819 punct ",",20
R5230 T9837 T9810 advcl phosphorylated,skimmed
R5231 T9838 T9837 dobj Akt,phosphorylated
R5232 T9839 T9838 cc and,Akt
R5233 T9840 T9838 conj IκB-α,Akt
R5234 T9841 T9845 punct (,Inc.
R5235 T9842 T9845 compound Cell,Inc.
R5236 T9843 T9845 compound Signaling,Inc.
R5237 T9844 T9845 compound Technology,Inc.
R5238 T9845 T9838 conj Inc.,Akt
R5239 T9846 T9845 punct ",",Inc.
R5240 T9847 T9845 npadvmod Beverly,Inc.
R5241 T9848 T9847 punct ",",Beverly
R5242 T9849 T9847 conj MA,Beverly
R5243 T9850 T9849 punct ",",MA
R5244 T9851 T9849 npadvmod USA,MA
R5245 T9852 T9849 punct ),MA
R5246 T9853 T9845 punct ",",Inc.
R5247 T9854 T9837 prep diluted,phosphorylated
R5248 T9855 T9854 npadvmod 1:1000,diluted
R5249 T9856 T9855 prep in,1:1000
R5250 T9857 T9858 nummod 5,%
R5251 T9858 T9859 compound %,BSA/TBS-T
R5252 T9859 T9856 pobj BSA/TBS-T,in
R5253 T9860 T9862 punct ",",added
R5254 T9861 T9862 auxpass were,added
R5255 T9862 T9806 conj added,preincubated
R5256 T9863 T9862 cc and,added
R5257 T9864 T9862 conj incubated,added
R5258 T9865 T9864 advmod overnight,incubated
R5259 T9866 T9864 prep at,incubated
R5260 T9867 T9868 nummod 4,°
R5261 T9868 T9866 pobj °,at
R5262 T9869 T9862 nsubj C,added
R5263 T9870 T9862 punct .,added
R5264 T9871 T9883 prep After,added
R5265 T9872 T9871 pcomp washing,After
R5266 T9873 T9874 nummod four,times
R5267 T9874 T9872 dobj times,washing
R5268 T9875 T9872 prep with,washing
R5269 T9876 T9875 pobj TBS-T,with
R5270 T9877 T9883 punct ",",added
R5271 T9878 T9881 amod horseradish,antibodies
R5272 T9879 T9881 amod peroxidase-conjugated,antibodies
R5273 T9880 T9881 amod secondary,antibodies
R5274 T9881 T9883 nsubjpass antibodies,added
R5275 T9882 T9883 auxpass were,added
R5276 T9883 T9883 ROOT added,added
R5277 T9884 T9883 cc and,added
R5278 T9885 T9883 conj allowed,added
R5279 T9886 T9887 aux to,incubate
R5280 T9887 T9885 xcomp incubate,allowed
R5281 T9888 T9887 prep for,incubate
R5282 T9889 T9890 nummod 1,hour
R5283 T9890 T9888 pobj hour,for
R5284 T9891 T9887 prep at,incubate
R5285 T9892 T9893 compound room,temperature
R5286 T9893 T9891 pobj temperature,at
R5287 T9894 T9883 punct .,added
R5288 T9895 T9902 prep After,detected
R5289 T9896 T9897 nsubj TBS-T,washing
R5290 T9897 T9895 pcomp washing,After
R5291 T9898 T9902 punct ",",detected
R5292 T9899 T9900 amod hybridized,bands
R5293 T9900 T9902 nsubjpass bands,detected
R5294 T9901 T9902 auxpass were,detected
R5295 T9902 T9902 ROOT detected,detected
R5296 T9903 T9902 prep with,detected
R5297 T9904 T9906 det the,chemiluminescence
R5298 T9905 T9906 amod enhanced,chemiluminescence
R5299 T9906 T9903 pobj chemiluminescence,with
R5300 T9907 T9906 punct (,chemiluminescence
R5301 T9908 T9906 appos ECL,chemiluminescence
R5302 T9909 T9911 punct ),kit
R5303 T9910 T9911 compound detection,kit
R5304 T9911 T9906 conj kit,chemiluminescence
R5305 T9912 T9911 cc and,kit
R5306 T9913 T9914 amod Hyperfilm-ECL,reagents
R5307 T9914 T9911 conj reagents,kit
R5308 T9915 T9917 punct (,Pharmacia
R5309 T9916 T9917 compound Amersham,Pharmacia
R5310 T9917 T9914 appos Pharmacia,reagents
R5311 T9918 T9914 punct ),reagents
R5312 T9919 T9902 punct .,detected
R5544 T10457 T10459 nmod Gel,assay
R5545 T10458 T10459 amod mobility-shift,assay
R5546 T10459 T10467 nsubjpass assay,extracted
R5547 T10460 T10459 prep of,assay
R5548 T10461 T10463 amod NF-κB,site
R5549 T10462 T10463 amod binding,site
R5550 T10463 T10465 compound site,proteins
R5551 T10464 T10465 compound Nuclear,proteins
R5552 T10465 T10460 pobj proteins,of
R5553 T10466 T10467 auxpass were,extracted
R5554 T10467 T10467 ROOT extracted,extracted
R5555 T10468 T10467 prep from,extracted
R5556 T10469 T10473 nmod about,PBMC
R5557 T10470 T10473 nummod 5,PBMC
R5558 T10471 T10473 nummod ×,PBMC
R5559 T10472 T10473 nummod 106,PBMC
R5560 T10473 T10468 pobj PBMC,from
R5561 T10474 T10467 punct .,extracted
R5562 T10475 T10476 compound Oligonucleotide,probes
R5563 T10476 T10502 nsubjpass probes,generated
R5564 T10477 T10476 acl encompassing,probes
R5565 T10478 T10481 det the,site
R5566 T10479 T10481 amod NF-κB,site
R5567 T10480 T10481 amod binding,site
R5568 T10481 T10477 dobj site,encompassing
R5569 T10482 T10481 prep of,site
R5570 T10483 T10486 det the,promoter
R5571 T10484 T10486 amod human,promoter
R5572 T10485 T10486 compound IL-17,promoter
R5573 T10486 T10482 pobj promoter,of
R5574 T10487 T10486 punct (,promoter
R5575 T10488 T10486 appos 5,promoter
R5576 T10489 T10486 punct ',promoter
R5577 T10490 T10502 punct -,generated
R5578 T10491 T10502 nsubjpass ATG,generated
R5579 T10492 T10502 nsubjpass ACC,generated
R5580 T10493 T10495 compound TGG,TAC
R5581 T10494 T10495 compound AAA,TAC
R5582 T10495 T10502 nsubjpass TAC,generated
R5583 T10496 T10497 compound CCA,AAA
R5584 T10497 T10498 compound AAA,TTC-3
R5585 T10498 T10495 appos TTC-3,TAC
R5586 T10499 T10495 punct ',TAC
R5587 T10500 T10495 punct ),TAC
R5588 T10501 T10502 auxpass were,generated
R5589 T10502 T10502 ROOT generated,generated
R5590 T10503 T10502 agent by,generated
R5591 T10504 T10503 pobj 5,by
R5592 T10505 T10504 punct ',5
R5593 T10506 T10507 punct -,end
R5594 T10507 T10508 compound end,labeling
R5595 T10508 T10502 dobj labeling,generated
R5596 T10509 T10508 prep of,labeling
R5597 T10510 T10512 det the,strand
R5598 T10511 T10512 compound sense,strand
R5599 T10512 T10509 pobj strand,of
R5600 T10513 T10512 prep with,strand
R5601 T10514 T10517 compound [,dATP
R5602 T10515 T10517 compound γ-32P,dATP
R5603 T10516 T10517 compound ],dATP
R5604 T10517 T10513 pobj dATP,with
R5605 T10518 T10520 punct (,Pharmacia
R5606 T10519 T10520 compound Amersham,Pharmacia
R5607 T10520 T10517 appos Pharmacia,dATP
R5608 T10521 T10520 punct ),Pharmacia
R5609 T10522 T10517 cc and,dATP
R5610 T10523 T10525 nummod T4,kinase
R5611 T10524 T10525 compound polynucleotide,kinase
R5612 T10525 T10517 conj kinase,dATP
R5613 T10526 T10525 punct (,kinase
R5614 T10527 T10525 appos TaKaRa,kinase
R5615 T10528 T10525 punct ),kinase
R5616 T10529 T10502 punct .,generated
R5617 T10530 T10531 compound Unincorporated,nucleotides
R5618 T10531 T10533 nsubjpass nucleotides,removed
R5619 T10532 T10533 auxpass were,removed
R5620 T10533 T10533 ROOT removed,removed
R5621 T10534 T10533 agent by,removed
R5622 T10535 T10538 nmod NucTrap,columns
R5623 T10536 T10537 compound probe,purification
R5624 T10537 T10538 compound purification,columns
R5625 T10538 T10534 pobj columns,by
R5626 T10539 T10538 punct (,columns
R5627 T10540 T10538 appos Stratagene,columns
R5628 T10541 T10540 punct ",",Stratagene
R5629 T10542 T10543 compound La,Jolla
R5630 T10543 T10540 conj Jolla,Stratagene
R5631 T10544 T10543 punct ",",Jolla
R5632 T10545 T10543 conj CA,Jolla
R5633 T10546 T10545 punct ",",CA
R5634 T10547 T10545 conj USA,CA
R5635 T10548 T10538 punct ),columns
R5636 T10549 T10533 punct .,removed
R5637 T10550 T10551 compound Nuclear,extracts
R5638 T10551 T10559 nsubjpass extracts,incubated
R5639 T10552 T10554 punct (,μg
R5640 T10553 T10554 nummod 2,μg
R5641 T10554 T10551 appos μg,extracts
R5642 T10555 T10554 prep of,μg
R5643 T10556 T10555 pobj protein,of
R5644 T10557 T10554 punct ),μg
R5645 T10558 T10559 auxpass were,incubated
R5646 T10559 T10559 ROOT incubated,incubated
R5647 T10560 T10559 prep with,incubated
R5648 T10561 T10563 amod radiolabeled,probes
R5649 T10562 T10563 compound DNA,probes
R5650 T10563 T10560 pobj probes,with
R5651 T10564 T10566 punct (,ng
R5652 T10565 T10566 nummod 10,ng
R5653 T10566 T10563 appos ng,probes
R5654 T10567 T10566 punct ;,ng
R5655 T10568 T10569 nummod "100,000",c.p.m.
R5656 T10569 T10566 appos c.p.m.,ng
R5657 T10570 T10566 punct ),ng
R5658 T10571 T10566 prep for,ng
R5659 T10572 T10573 nummod 30,min
R5660 T10573 T10571 pobj min,for
R5661 T10574 T10573 prep at,min
R5662 T10575 T10576 compound room,temperature
R5663 T10576 T10574 pobj temperature,at
R5664 T10577 T10573 prep in,min
R5665 T10578 T10579 nummod 20,μl
R5666 T10579 T10577 pobj μl,in
R5667 T10580 T10579 prep of,μl
R5668 T10581 T10582 amod binding,buffer
R5669 T10582 T10580 pobj buffer,of
R5670 T10583 T10582 acl consisting,buffer
R5671 T10584 T10583 prep of,consisting
R5672 T10585 T10587 nummod 20,Tris-HCl
R5673 T10586 T10587 compound mM,Tris-HCl
R5674 T10587 T10584 pobj Tris-HCl,of
R5675 T10588 T10587 punct ",",Tris-HCl
R5676 T10589 T10587 appos pH,Tris-HCl
R5677 T10590 T10589 nummod 7.9,pH
R5678 T10591 T10589 punct ",",pH
R5679 T10592 T10594 nummod 50,KCl
R5680 T10593 T10594 compound mM,KCl
R5681 T10594 T10587 appos KCl,Tris-HCl
R5682 T10595 T10594 punct ",",KCl
R5683 T10596 T10598 nummod 1,dithiothreitol
R5684 T10597 T10598 compound mM,dithiothreitol
R5685 T10598 T10594 appos dithiothreitol,KCl
R5686 T10599 T10598 punct ",",dithiothreitol
R5687 T10600 T10602 nummod 0.5,EDTA
R5688 T10601 T10602 compound mM,EDTA
R5689 T10602 T10598 appos EDTA,dithiothreitol
R5690 T10603 T10602 punct ",",EDTA
R5691 T10604 T10605 nummod 5,%
R5692 T10605 T10606 compound %,glycerol
R5693 T10606 T10602 appos glycerol,EDTA
R5694 T10607 T10606 punct ",",glycerol
R5695 T10608 T10609 nummod 1,mg/ml
R5696 T10609 T10610 compound mg/ml,BSA
R5697 T10610 T10602 appos BSA,EDTA
R5698 T10611 T10602 punct ",",EDTA
R5699 T10612 T10613 nummod 0.2,%
R5700 T10613 T10615 compound %,P40
R5701 T10614 T10615 compound Nonidet,P40
R5702 T10615 T10602 conj P40,EDTA
R5703 T10616 T10615 cc and,P40
R5704 T10617 T10618 nummod 50,ng
R5705 T10618 T10621 compound ng,poly
R5706 T10619 T10621 compound /,poly
R5707 T10620 T10621 compound μl,poly
R5708 T10621 T10615 conj poly,P40
R5709 T10622 T10623 punct (,dI-dC
R5710 T10623 T10621 parataxis dI-dC,poly
R5711 T10624 T10623 punct ),dI-dC
R5712 T10625 T10559 punct .,incubated
R5713 T10626 T10628 nsubjpass Samples,subjected
R5714 T10627 T10628 auxpass were,subjected
R5715 T10628 T10628 ROOT subjected,subjected
R5716 T10629 T10628 prep to,subjected
R5717 T10630 T10629 pobj electrophoresis,to
R5718 T10631 T10630 prep on,electrophoresis
R5719 T10632 T10631 pcomp nondenaturing,on
R5720 T10633 T10634 nummod 5,%
R5721 T10634 T10636 compound %,gels
R5722 T10635 T10636 compound polyacrylamide,gels
R5723 T10636 T10632 dobj gels,nondenaturing
R5724 T10637 T10632 prep in,nondenaturing
R5725 T10638 T10641 nummod 0.5,buffer
R5726 T10639 T10641 nummod ×,buffer
R5727 T10640 T10641 compound Tris-borate-EDTA,buffer
R5728 T10641 T10637 pobj buffer,in
R5729 T10642 T10643 punct (,pH
R5730 T10643 T10629 pobj pH,to
R5731 T10644 T10643 nummod 8.0,pH
R5732 T10645 T10643 punct ),pH
R5733 T10646 T10628 prep at,subjected
R5734 T10647 T10649 nummod 100,Gels
R5735 T10648 T10649 compound V.,Gels
R5736 T10649 T10646 pobj Gels,at
R5737 T10650 T10651 auxpass were,dried
R5738 T10651 T10628 conj dried,subjected
R5739 T10652 T10651 prep under,dried
R5740 T10653 T10652 pobj vacuum,under
R5741 T10654 T10651 cc and,dried
R5742 T10655 T10651 conj exposed,dried
R5743 T10656 T10655 prep to,exposed
R5744 T10657 T10659 compound Kodak,film
R5745 T10658 T10659 compound X-OMAT,film
R5746 T10659 T10656 pobj film,to
R5747 T10660 T10655 prep at,exposed
R5748 T10661 T10662 nummod -70,°
R5749 T10662 T10660 pobj °,at
R5750 T10663 T10655 npadvmod C,exposed
R5751 T10664 T10655 prep with,exposed
R5752 T10665 T10666 amod intensifying,screens
R5753 T10666 T10664 pobj screens,with
R5754 T10667 T10628 punct .,subjected
R5755 T10668 T10670 compound Rabbit,antibodies
R5756 T10669 T10670 compound polyclonal,antibodies
R5757 T10670 T10673 nsubj antibodies,subunits
R5758 T10671 T10670 prep against,antibodies
R5759 T10672 T10671 pobj NF-κB,against
R5760 T10673 T10673 ROOT subunits,subunits
R5761 T10674 T10673 dobj p50,subunits
R5762 T10675 T10674 punct ",",p50
R5763 T10676 T10674 conj p65,p50
R5764 T10677 T10679 cc and,were
R5765 T10678 T10679 nsubj c-Rel,were
R5766 T10679 T10679 ROOT were,were
R5767 T10680 T10679 prep from,were
R5768 T10681 T10683 compound Santa,Biotechnology
R5769 T10682 T10683 compound Cruz,Biotechnology
R5770 T10683 T10680 pobj Biotechnology,from
R5771 T10684 T10683 punct (,Biotechnology
R5772 T10685 T10686 compound Santa,Cruz
R5773 T10686 T10683 appos Cruz,Biotechnology
R5774 T10687 T10683 punct ",",Biotechnology
R5775 T10688 T10683 conj CA,Biotechnology
R5776 T10689 T10688 punct ",",CA
R5777 T10690 T10688 conj USA,CA
R5778 T10691 T10679 punct ),were
R5779 T10692 T10679 punct .,were
R5856 T10901 T10902 compound Cell,viability
R5857 T10902 T10902 ROOT viability,viability
R5858 T10903 T10908 punct (,assay
R5859 T10904 T10908 nmod Trypan,assay
R5860 T10905 T10906 amod blue,dye
R5861 T10906 T10908 nmod dye,assay
R5862 T10907 T10908 compound exclusion,assay
R5863 T10908 T10902 appos assay,viability
R5864 T10909 T10902 punct ),viability
R5865 T10910 T10922 prep For,used
R5866 T10911 T10912 compound cell,viability
R5867 T10912 T10913 compound viability,assays
R5868 T10913 T10910 pobj assays,For
R5869 T10914 T10922 punct ",",used
R5870 T10915 T10920 det the,method
R5871 T10916 T10920 amod trypan,method
R5872 T10917 T10918 amod blue,dye
R5873 T10918 T10919 compound dye,exclusion
R5874 T10919 T10920 compound exclusion,method
R5875 T10920 T10922 nsubjpass method,used
R5876 T10921 T10922 auxpass was,used
R5877 T10922 T10922 ROOT used,used
R5878 T10923 T10924 aux to,evaluate
R5879 T10924 T10922 xcomp evaluate,used
R5880 T10925 T10926 det the,potential
R5881 T10926 T10924 dobj potential,evaluate
R5882 T10927 T10926 prep of,potential
R5883 T10928 T10930 amod direct,effect
R5884 T10929 T10930 amod cytotoxic,effect
R5885 T10930 T10927 pobj effect,of
R5886 T10931 T10930 prep of,effect
R5887 T10932 T10931 pobj inhibitors,of
R5888 T10933 T10932 prep on,inhibitors
R5889 T10934 T10933 pobj cells,on
R5890 T10935 T10922 punct .,used
R5891 T10936 T10945 prep After,harvested
R5892 T10937 T10936 pobj incubation,After
R5893 T10938 T10937 prep for,incubation
R5894 T10939 T10940 nummod 24,hours
R5895 T10940 T10938 pobj hours,for
R5896 T10941 T10945 punct ",",harvested
R5897 T10942 T10943 det the,cells
R5898 T10943 T10945 nsubjpass cells,harvested
R5899 T10944 T10945 auxpass were,harvested
R5900 T10945 T10945 ROOT harvested,harvested
R5901 T10946 T10945 cc and,harvested
R5902 T10947 T10950 det the,viability
R5903 T10948 T10949 compound percentage,cell
R5904 T10949 T10950 compound cell,viability
R5905 T10950 T10952 nsubjpass viability,calculated
R5906 T10951 T10952 auxpass was,calculated
R5907 T10952 T10945 conj calculated,harvested
R5908 T10953 T10952 prep with,calculated
R5909 T10954 T10955 det the,formula
R5910 T10955 T10953 pobj formula,with
R5911 T10956 T10957 nummod 100,×
R5912 T10957 T10972 npadvmod ×,]
R5913 T10958 T10959 punct (,number
R5914 T10959 T10972 nmod number,]
R5915 T10960 T10959 prep of,number
R5916 T10961 T10962 amod viable,cells/number
R5917 T10962 T10960 pobj cells/number,of
R5918 T10963 T10962 prep of,cells/number
R5919 T10964 T10965 preconj both,viable
R5920 T10965 T10968 amod viable,cells
R5921 T10966 T10965 cc and,viable
R5922 T10967 T10965 conj dead,viable
R5923 T10968 T10963 pobj cells,of
R5924 T10969 T10959 punct ),number
R5925 T10970 T10972 quantmod [,]
R5926 T10971 T10972 nummod 21,]
R5927 T10972 T10952 conj ],calculated
R5928 T10973 T10952 punct .,calculated
R5957 T11046 T11047 compound Statistical,analysis
R5958 T11047 T11048 compound analysis,Data
R5959 T11048 T11050 nsubjpass Data,expressed
R5960 T11049 T11050 auxpass are,expressed
R5961 T11050 T11050 ROOT expressed,expressed
R5962 T11051 T11052 mark as,means
R5963 T11052 T11050 advcl means,expressed
R5964 T11053 T11054 compound ±,SEM
R5965 T11054 T11052 dobj SEM,means
R5966 T11055 T11054 punct .,SEM
R5967 T11056 T11057 compound Statistical,analysis
R5968 T11057 T11059 nsubjpass analysis,performed
R5969 T11058 T11059 auxpass was,performed
R5970 T11059 T11059 ROOT performed,performed
R5971 T11060 T11059 prep with,performed
R5972 T11061 T11060 pobj Student,with
R5973 T11062 T11061 case 's,Student
R5974 T11063 T11061 amod t-test,Student
R5975 T11064 T11063 prep for,t-test
R5976 T11065 T11066 amod matched,pairs
R5977 T11066 T11064 pobj pairs,for
R5978 T11067 T11059 punct .,performed
R5979 T11068 T11069 nsubj P,values
R5980 T11069 T11069 ROOT values,values
R5981 T11070 T11072 amod less,0.05
R5982 T11071 T11072 quantmod than,0.05
R5983 T11072 T11074 nsubjpass 0.05,considered
R5984 T11073 T11074 auxpass were,considered
R5985 T11074 T11069 ccomp considered,values
R5986 T11075 T11074 oprd significant,considered
R5987 T11076 T11069 punct .,values
R599 T1443 T1445 amod Increased,production
R600 T1444 T1445 amod interleukin-17,production
R601 T1445 T1445 ROOT production,production
R602 T1446 T1445 prep via,production
R603 T1447 T1449 det a,3-kinase/Akt
R604 T1448 T1449 compound phosphoinositide,3-kinase/Akt
R605 T1449 T1446 pobj 3-kinase/Akt,via
R606 T1450 T1449 cc and,3-kinase/Akt
R607 T1451 T1452 amod nuclear,factor
R608 T1452 T1449 conj factor,3-kinase/Akt
R609 T1453 T1445 punct κ,production
R610 T1454 T1455 amod B-dependent,pathway
R611 T1455 T1465 nsubjpass pathway,recognized
R612 T1456 T1455 prep in,pathway
R613 T1457 T1456 pobj patients,in
R614 T1458 T1457 prep with,patients
R615 T1459 T1460 compound rheumatoid,arthritis
R616 T1460 T1458 pobj arthritis,with
R6160 T11434 T11435 compound IL-17,production
R6161 T11435 T11451 nsubjpass production,separated
R6162 T11436 T11435 prep in,production
R6163 T11437 T11436 pobj PBMC,in
R6164 T11438 T11435 prep from,production
R6165 T11439 T11438 pobj patients,from
R6166 T11440 T11439 prep with,patients
R6167 T11441 T11440 pobj RA,with
R6168 T11442 T11439 punct ",",patients
R6169 T11443 T11451 nsubjpass patients,separated
R617 T1461 T1462 amod Inflammatory,mediators
R6170 T11444 T11443 prep with,patients
R6171 T11445 T11444 pobj OA,with
R6172 T11446 T11445 cc and,OA
R6173 T11447 T11448 amod normal,individuals
R6174 T11448 T11445 conj individuals,OA
R6175 T11449 T11445 conj PBMC,OA
R6176 T11450 T11451 auxpass were,separated
R6177 T11451 T11480 ccomp separated,determined
R6178 T11452 T11451 cc and,separated
R6179 T11453 T11451 conj cultured,separated
R618 T1462 T1465 nsubjpass mediators,recognized
R6180 T11454 T11453 prep with,cultured
R6181 T11455 T11454 pobj PHA,with
R6182 T11456 T11455 punct (,PHA
R6183 T11457 T11458 nummod 5,μg
R6184 T11458 T11460 compound μg,ml
R6185 T11459 T11460 compound /,ml
R6186 T11460 T11455 appos ml,PHA
R6187 T11461 T11455 punct ),PHA
R6188 T11462 T11453 prep from,cultured
R6189 T11463 T11462 pobj patients,from
R619 T1463 T1465 aux have,recognized
R6190 T11464 T11463 prep with,patients
R6191 T11465 T11464 pobj RA,with
R6192 T11466 T11463 punct ",",patients
R6193 T11467 T11463 appos patients,patients
R6194 T11468 T11467 prep with,patients
R6195 T11469 T11468 pobj OA,with
R6196 T11470 T11469 punct ",",OA
R6197 T11471 T11469 cc and,OA
R6198 T11472 T11474 amod age-matched,controls
R6199 T11473 T11474 amod normal,controls
R620 T1464 T1465 auxpass been,recognized
R6200 T11474 T11469 conj controls,OA
R6201 T11475 T11480 punct ;,determined
R6202 T11476 T11477 compound IL-17,levels
R6203 T11477 T11480 nsubjpass levels,determined
R6204 T11478 T11480 auxpass were,determined
R6205 T11479 T11480 advmod then,determined
R6206 T11480 T11480 ROOT determined,determined
R6207 T11481 T11480 prep in,determined
R6208 T11482 T11484 det the,supernatants
R6209 T11483 T11484 compound culture,supernatants
R621 T1465 T1465 ROOT recognized,recognized
R6210 T11484 T11481 pobj supernatants,in
R6211 T11485 T11480 punct (,determined
R6212 T11486 T11488 nmod Fig.,)
R6213 T11487 T11486 nummod 1,Fig.
R6214 T11488 T11480 punct ),determined
R6215 T11489 T11480 punct .,determined
R6216 T11490 T11497 mark Although,were
R6217 T11491 T11492 det the,amounts
R6218 T11492 T11497 nsubj amounts,were
R6219 T11493 T11492 prep of,amounts
R622 T1466 T1465 prep as,recognized
R6220 T11494 T11496 amod basal,secretion
R6221 T11495 T11496 compound IL-17,secretion
R6222 T11496 T11493 pobj secretion,of
R6223 T11497 T11530 advcl were,was
R6224 T11498 T11497 neg not,were
R6225 T11499 T11497 acomp different,were
R6226 T11500 T11499 prep between,different
R6227 T11501 T11506 nmod RA,controls
R6228 T11502 T11501 punct ",",RA
R6229 T11503 T11501 conj OA,RA
R623 T1467 T1466 pcomp being,as
R6230 T11504 T11503 cc and,OA
R6231 T11505 T11506 amod normal,controls
R6232 T11506 T11500 pobj controls,between
R6233 T11507 T11499 punct (,different
R6234 T11508 T11509 nummod 62,±
R6235 T11509 T11521 npadvmod ±,respectively
R6236 T11510 T11509 nummod 31,±
R6237 T11511 T11509 punct ",",±
R6238 T11512 T11518 quantmod 43,10
R6239 T11513 T11518 quantmod ±,10
R624 T1468 T1467 acomp important,being
R6240 T11514 T11513 nummod 19,±
R6241 T11515 T11513 cc and,±
R6242 T11516 T11518 quantmod 43,10
R6243 T11517 T11518 quantmod ±,10
R6244 T11518 T11519 nummod 10,pg/ml
R6245 T11519 T11509 appos pg/ml,±
R6246 T11520 T11509 punct ",",±
R6247 T11521 T11497 advmod respectively,were
R6248 T11522 T11497 punct ),were
R6249 T11523 T11530 punct ",",was
R625 T1469 T1467 prep in,being
R6250 T11524 T11526 det the,production
R6251 T11525 T11526 compound IL-17,production
R6252 T11526 T11530 nsubj production,was
R6253 T11527 T11526 acl stimulated,production
R6254 T11528 T11527 agent by,stimulated
R6255 T11529 T11528 pobj PHA,by
R6256 T11530 T11530 ROOT was,was
R6257 T11531 T11532 advmod significantly,higher
R6258 T11532 T11530 acomp higher,was
R6259 T11533 T11530 prep in,was
R626 T1470 T1471 det the,pathogenesis
R6260 T11534 T11535 compound RA,PBMC
R6261 T11535 T11533 pobj PBMC,in
R6262 T11536 T11530 prep than,was
R6263 T11537 T11536 prep in,than
R6264 T11538 T11537 pobj those,in
R6265 T11539 T11538 prep from,those
R6266 T11540 T11539 pobj OA,from
R6267 T11541 T11540 cc and,OA
R6268 T11542 T11540 conj controls,OA
R6269 T11543 T11540 punct (,OA
R627 T1471 T1469 pobj pathogenesis,in
R6270 T11544 T11546 nummod 768,295
R6271 T11545 T11546 nummod ±,295
R6272 T11546 T11554 npadvmod 295,<
R6273 T11547 T11546 prep versus,295
R6274 T11548 T11550 nummod 463,211
R6275 T11549 T11550 nummod ±,211
R6276 T11550 T11547 pobj 211,versus
R6277 T11551 T11553 amod pg/ml,P
R6278 T11552 T11553 compound [,P
R6279 T11553 T11554 nsubj P,<
R628 T1472 T1471 prep of,pathogenesis
R6280 T11554 T11564 dep <,<
R6281 T11555 T11556 nummod 0.05,]
R6282 T11556 T11554 dobj ],<
R6283 T11557 T11556 cc and,]
R6284 T11558 T11560 nummod 241,29
R6285 T11559 T11560 nummod ±,29
R6286 T11560 T11563 nummod 29,P
R6287 T11561 T11563 compound pg/ml,P
R6288 T11562 T11563 compound [,P
R6289 T11563 T11556 conj P,]
R629 T1473 T1474 amod rheumatoid,arthritis
R6290 T11564 T11530 conj <,was
R6291 T11565 T11566 nummod 0.001,]
R6292 T11566 T11564 dobj ],<
R6293 T11567 T11564 punct ),<
R6294 T11568 T11530 punct .,was
R630 T1474 T1472 pobj arthritis,of
R631 T1475 T1476 punct (,RA
R632 T1476 T1471 appos RA,pathogenesis
R633 T1477 T1471 punct ),pathogenesis
R634 T1478 T1465 punct .,recognized
R635 T1479 T1483 nmod Interleukin,-17
R636 T1480 T1481 punct (,IL
R637 T1481 T1479 appos IL,Interleukin
R638 T1482 T1483 punct ),-17
R639 T1483 T1484 nsubj -17,is
R640 T1484 T1484 ROOT is,is
R641 T1485 T1487 det an,regulator
R642 T1486 T1487 amod important,regulator
R643 T1487 T1484 attr regulator,is
R644 T1488 T1487 prep of,regulator
R645 T1489 T1492 amod immune,responses
R646 T1490 T1489 cc and,immune
R647 T1491 T1489 conj inflammatory,immune
R648 T1492 T1488 pobj responses,of
R649 T1493 T1492 punct ",",responses
R650 T1494 T1492 prep including,responses
R651 T1495 T1496 det the,induction
R652 T1496 T1494 pobj induction,including
R653 T1497 T1496 prep of,induction
R654 T1498 T1499 amod proinflammatory,cytokines
R6542 T12114 T12116 amod Increased,production
R6543 T12115 T12116 amod IL-17,production
R6544 T12116 T12148 nsubj production,investigated
R6545 T12117 T12116 prep in,production
R6546 T12118 T12117 pobj PBMC,in
R6547 T12119 T12118 prep of,PBMC
R6548 T12120 T12119 pobj patients,of
R6549 T12121 T12120 prep with,patients
R655 T1499 T1497 pobj cytokines,of
R6550 T12122 T12121 pobj RA,with
R6551 T12123 T12116 prep by,production
R6552 T12124 T12148 advcl anti-CD3,investigated
R6553 T12125 T12124 cc and/or,anti-CD3
R6554 T12126 T12124 conj anti-CD28,anti-CD3
R6555 T12127 T12126 punct ",",anti-CD28
R6556 T12128 T12124 cc and,anti-CD3
R6557 T12129 T12124 conj PHA,anti-CD3
R6558 T12130 T12134 mark Because,known
R6559 T12131 T12134 nsubjpass IL-17,known
R656 T1500 T1499 cc and,cytokines
R6560 T12132 T12134 auxpass was,known
R6561 T12133 T12134 advmod already,known
R6562 T12134 T12148 advcl known,investigated
R6563 T12135 T12134 prep from,known
R6564 T12136 T12137 amod earlier,reports
R6565 T12137 T12135 pobj reports,from
R6566 T12138 T12140 aux to,produced
R6567 T12139 T12140 auxpass be,produced
R6568 T12140 T12137 acl produced,reports
R6569 T12141 T12140 advmod mainly,produced
R657 T1501 T1503 amod osteoclastic,resorption
R6570 T12142 T12140 agent by,produced
R6571 T12143 T12145 amod activated,cells
R6572 T12144 T12145 compound T,cells
R6573 T12145 T12142 pobj cells,by
R6574 T12146 T12148 punct ",",investigated
R6575 T12147 T12148 nsubj we,investigated
R6576 T12148 T12148 ROOT investigated,investigated
R6577 T12149 T12150 det the,effect
R6578 T12150 T12148 dobj effect,investigated
R6579 T12151 T12150 prep of,effect
R658 T1502 T1503 compound bone,resorption
R6580 T12152 T12153 amod different,concentrations
R6581 T12153 T12151 pobj concentrations,of
R6582 T12154 T12153 prep of,concentrations
R6583 T12155 T12154 pobj anti-CD3,of
R6584 T12156 T12164 punct (,ml
R6585 T12157 T12162 nummod 1,μg
R6586 T12158 T12159 punct ",",5
R6587 T12159 T12157 prep 5,1
R6588 T12160 T12157 cc and,1
R6589 T12161 T12157 conj 10,1
R659 T1503 T1499 conj resorption,cytokines
R6590 T12162 T12164 nmod μg,ml
R6591 T12163 T12164 compound /,ml
R6592 T12164 T12155 appos ml,anti-CD3
R6593 T12165 T12164 punct ),ml
R6594 T12166 T12148 prep as,investigated
R6595 T12167 T12170 det a,activation
R6596 T12168 T12170 compound T,activation
R6597 T12169 T12170 compound cell,activation
R6598 T12170 T12166 pobj activation,as
R6599 T12171 T12170 punct ",",activation
R660 T1504 T1484 punct .,is
R6600 T12172 T12173 nsubj which,showed
R6601 T12173 T12170 relcl showed,activation
R6602 T12174 T12176 det a,increase
R6603 T12175 T12176 amod dose-dependent,increase
R6604 T12176 T12173 dobj increase,showed
R6605 T12177 T12176 prep in,increase
R6606 T12178 T12179 compound IL-17,levels
R6607 T12179 T12177 pobj levels,in
R6608 T12180 T12179 punct (,levels
R6609 T12181 T12179 appos data,levels
R661 T1505 T1516 nsubjpass Evidence,demonstrated
R6610 T12182 T12183 neg not,shown
R6611 T12183 T12181 acl shown,data
R6612 T12184 T12181 punct ),data
R6613 T12185 T12148 punct .,investigated
R6614 T12186 T12193 prep On,chose
R6615 T12187 T12188 det the,basis
R6616 T12188 T12186 pobj basis,On
R6617 T12189 T12188 prep of,basis
R6618 T12190 T12189 pobj this,of
R6619 T12191 T12193 punct ",",chose
R662 T1509 T1508 cc and,expression
R6620 T12192 T12193 nsubj we,chose
R6621 T12193 T12193 ROOT chose,chose
R6622 T12194 T12195 nummod 10,μg
R6623 T12195 T12197 compound μg,ml
R6624 T12196 T12197 compound /,ml
R6625 T12197 T12193 dobj ml,chose
R6626 T12198 T12193 prep as,chose
R6627 T12199 T12201 det a,concentration
R6628 T12200 T12201 compound stimulation,concentration
R6629 T12201 T12198 pobj concentration,as
R663 T1510 T1511 amod proinflammatory,activity
R6630 T12202 T12201 prep for,concentration
R6631 T12203 T12202 pobj anti-CD3,for
R6632 T12204 T12193 punct .,chose
R6633 T12205 T12206 mark As,shown
R6634 T12206 T12227 advcl shown,produced
R6635 T12207 T12206 prep in,shown
R6636 T12208 T12207 pobj Table,in
R6637 T12209 T12208 nummod 1,Table
R6638 T12210 T12227 punct ",",produced
R6639 T12211 T12215 nmod anti-CD3,production
R664 T1511 T1508 conj activity,expression
R6640 T12212 T12213 advmod significantly,upregulated
R6641 T12213 T12215 nmod upregulated,production
R6642 T12214 T12215 compound IL-17,production
R6643 T12215 T12227 nsubj production,produced
R6644 T12216 T12215 prep up,production
R6645 T12217 T12216 prep to,up
R6646 T12218 T12217 pobj 3.7-fold,to
R6647 T12219 T12215 punct ",",production
R6648 T12220 T12215 cc and,production
R6649 T12221 T12222 det the,combination
R665 T1512 T1508 prep of,expression
R6650 T12222 T12215 conj combination,production
R6651 T12223 T12222 prep of,combination
R6652 T12224 T12227 amod anti-CD28,produced
R6653 T12225 T12224 cc and,anti-CD28
R6654 T12226 T12224 conj anti-CD3,anti-CD28
R6655 T12227 T12227 ROOT produced,produced
R6656 T12228 T12229 advmod more,IL-17
R6657 T12229 T12227 dobj IL-17,produced
R6658 T12230 T12235 punct (,fold
R6659 T12231 T12232 advmod approximately,1.3-1
R666 T1513 T1512 pobj IL-17,of
R6660 T12232 T12235 nummod 1.3-1,fold
R6661 T12233 T12235 punct .5,fold
R6662 T12234 T12235 punct -,fold
R6663 T12235 T12229 appos fold,IL-17
R6664 T12236 T12235 punct ),fold
R6665 T12237 T12229 prep than,IL-17
R6666 T12238 T12237 pobj anti-CD3,than
R6667 T12239 T12238 advmod alone,anti-CD3
R6668 T12240 T12227 punct .,produced
R6669 T12241 T12253 advmod Furthermore,increased
R667 T1514 T1516 aux has,demonstrated
R6670 T12242 T12253 punct ",",increased
R6671 T12243 T12244 advmod when,incubated
R6672 T12244 T12253 advcl incubated,increased
R6673 T12245 T12244 prep with,incubated
R6674 T12246 T12247 compound T,cell
R6675 T12247 T12248 compound cell,mitogens
R6676 T12248 T12245 pobj mitogens,with
R6677 T12249 T12250 amod such,as
R6678 T12250 T12248 prep as,mitogens
R6679 T12251 T12250 pobj PHA,as
R668 T1515 T1516 auxpass been,demonstrated
R6680 T12252 T12253 punct ",",increased
R6681 T12253 T12255 amod increased,production
R6682 T12254 T12255 compound IL-17,production
R6683 T12255 T12256 nsubj production,was
R6684 T12256 T12256 ROOT was,was
R6685 T12257 T12258 advmod more,pronounced
R6686 T12258 T12256 acomp pronounced,was
R6687 T12259 T12258 prep than,pronounced
R6688 T12260 T12259 prep with,than
R6689 T12261 T12260 pobj anti-CD3,with
R669 T1516 T1516 ROOT demonstrated,demonstrated
R6690 T12262 T12261 cc and,anti-CD3
R6691 T12263 T12261 conj anti-CD28,anti-CD3
R6692 T12264 T12263 punct (,anti-CD28
R6693 T12265 T12266 nummod 588,±
R6694 T12266 T12263 appos ±,anti-CD28
R6695 T12267 T12266 nummod 85,±
R6696 T12268 T12267 prep versus,85
R6697 T12269 T12272 nummod 211,pg/ml
R6698 T12270 T12272 nmod ±,pg/ml
R6699 T12271 T12272 nummod 1,pg/ml
R670 T1517 T1516 prep in,demonstrated
R6700 T12272 T12268 pobj pg/ml,versus
R6701 T12273 T12276 punct ;,0.05
R6702 T12274 T12275 compound P,<
R6703 T12275 T12276 compound <,0.05
R6704 T12276 T12256 npadvmod 0.05,was
R6705 T12277 T12276 punct ),0.05
R6706 T12278 T12256 punct .,was
R671 T1518 T1519 compound RA,synovium
R672 T1519 T1517 pobj synovium,in
R673 T1520 T1517 cc and,in
R674 T1521 T1517 conj in,in
R675 T1522 T1523 compound animal,models
R676 T1523 T1521 pobj models,in
R677 T1524 T1523 prep of,models
R678 T1525 T1524 pobj RA,of
R679 T1526 T1516 punct .,demonstrated
R680 T1527 T1537 mark Although,reported
R681 T1528 T1529 det some,cytokines
R682 T1529 T1537 nsubjpass cytokines,reported
R683 T1530 T1529 punct (,cytokines
R684 T1531 T1529 appos IL-15,cytokines
R685 T1532 T1531 cc and,IL-15
R686 T1533 T1531 conj IL-23,IL-15
R687 T1534 T1529 punct ),cytokines
R688 T1535 T1537 aux have,reported
R689 T1536 T1537 auxpass been,reported
R690 T1537 T1551 advcl reported,remain
R691 T1538 T1539 aux to,regulate
R692 T1539 T1537 xcomp regulate,reported
R693 T1540 T1541 compound IL-17,production
R694 T1541 T1539 dobj production,regulate
R695 T1542 T1551 punct ",",remain
R696 T1543 T1546 det the,pathways
R697 T1544 T1545 nsubj intracellular,signaling
R698 T1545 T1546 compound signaling,pathways
R699 T1546 T1551 nsubj pathways,remain
R700 T1547 T1548 nsubj that,regulate
R701 T1548 T1546 relcl regulate,pathways
R702 T1549 T1550 compound IL-17,production
R7021 T12997 T13012 nsubj Regulation,include
R7022 T12998 T12997 prep of,Regulation
R7023 T12999 T13000 amod IL-17,production
R7024 T13000 T12998 pobj production,of
R7025 T13001 T13000 prep in,production
R7026 T13002 T13003 compound RA,PBMC
R7027 T13003 T13001 pobj PBMC,in
R7028 T13004 T12997 prep by,Regulation
R7029 T13005 T13006 amod inflammatory,cytokines
R703 T1550 T1548 dobj production,regulate
R7030 T13006 T13004 pobj cytokines,by
R7031 T13007 T13006 cc and,cytokines
R7032 T13008 T13006 conj chemokines,cytokines
R7033 T13009 T13012 mark Because,include
R7034 T13010 T13011 compound RA,PBMC
R7035 T13011 T13012 nsubj PBMC,include
R7036 T13012 T13012 ROOT include,include
R7037 T13013 T13015 amod several,types
R7038 T13014 T13015 compound cell,types
R7039 T13015 T13012 dobj types,include
R704 T1551 T1551 ROOT remain,remain
R7040 T13016 T13033 prep in,affected
R7041 T13017 T13016 pobj addition,in
R7042 T13018 T13017 prep to,addition
R7043 T13019 T13020 compound T,cells
R7044 T13020 T13018 pobj cells,to
R7045 T13021 T13033 punct ",",affected
R7046 T13022 T13024 det some,cytokines
R7047 T13023 T13024 amod inflammatory,cytokines
R7048 T13024 T13033 nsubj cytokines,affected
R7049 T13025 T13024 acl released,cytokines
R705 T1552 T1551 acomp unknown,remain
R7050 T13026 T13025 prep from,released
R7051 T13027 T13026 pobj macrophages,from
R7052 T13028 T13027 cc and,macrophages
R7053 T13029 T13030 amod other,lymphocytes
R7054 T13030 T13027 conj lymphocytes,macrophages
R7055 T13031 T13033 aux might,affected
R7056 T13032 T13033 aux have,affected
R7057 T13033 T13012 ccomp affected,include
R7058 T13034 T13035 det the,production
R7059 T13035 T13033 dobj production,affected
R706 T1553 T1551 punct .,remain
R7060 T13036 T13035 prep of,production
R7061 T13037 T13036 pobj IL-17,of
R7062 T13038 T13037 prep from,IL-17
R7063 T13039 T13040 compound T,cells
R7064 T13040 T13038 pobj cells,from
R7065 T13041 T13012 punct .,include
R7066 T13042 T13043 aux To,evaluate
R7067 T13043 T13055 advcl evaluate,tested
R7068 T13044 T13045 det the,effects
R7069 T13045 T13043 dobj effects,evaluate
R707 T1554 T1560 prep In,investigated
R7070 T13046 T13045 prep of,effects
R7071 T13047 T13048 amod inflammatory,cytokines
R7072 T13048 T13046 pobj cytokines,of
R7073 T13049 T13045 acl released,effects
R7074 T13050 T13049 agent by,released
R7075 T13051 T13052 amod activated,PBMC
R7076 T13052 T13050 pobj PBMC,by
R7077 T13053 T13055 punct ",",tested
R7078 T13054 T13055 nsubj we,tested
R7079 T13055 T13055 ROOT tested,tested
R708 T1555 T1557 det the,study
R7080 T13056 T13057 det the,effects
R7081 T13057 T13055 dobj effects,tested
R7082 T13058 T13057 prep of,effects
R7083 T13059 T13060 amod several,cytokines
R7084 T13060 T13058 pobj cytokines,of
R7085 T13061 T13060 cc and,cytokines
R7086 T13062 T13060 conj chemokines,cytokines
R7087 T13063 T13057 prep on,effects
R7088 T13064 T13065 compound IL-17,production
R7089 T13065 T13063 pobj production,on
R709 T1556 T1557 amod present,study
R7090 T13066 T13055 punct .,tested
R7091 T13067 T13068 nsubj We,detected
R7092 T13068 T13068 ROOT detected,detected
R7093 T13069 T13070 det an,increase
R7094 T13070 T13068 dobj increase,detected
R7095 T13071 T13070 prep in,increase
R7096 T13072 T13073 compound IL-17,level
R7097 T13073 T13071 pobj level,in
R7098 T13074 T13068 prep after,detected
R7099 T13075 T13074 pobj stimulation,after
R710 T1557 T1554 pobj study,In
R7100 T13076 T13068 prep with,detected
R7101 T13077 T13076 pobj IL-15,with
R7102 T13078 T13077 punct (,IL-15
R7103 T13079 T13080 nummod 10,ng/ml
R7104 T13080 T13077 appos ng/ml,IL-15
R7105 T13081 T13077 punct ),IL-15
R7106 T13082 T13068 punct ",",detected
R7107 T13083 T13112 mark whereas,were
R7108 T13084 T13112 prep with,were
R7109 T13085 T13084 pobj IL-1β,with
R711 T1558 T1560 punct ",",investigated
R7110 T13086 T13085 punct (,IL-1β
R7111 T13087 T13088 nummod 10,ng/ml
R7112 T13088 T13085 appos ng/ml,IL-1β
R7113 T13089 T13085 punct ),IL-1β
R7114 T13090 T13112 punct ",",were
R7115 T13091 T13109 nmod TNF-α,levels
R7116 T13092 T13094 punct (,ng/ml
R7117 T13093 T13094 nummod 10,ng/ml
R7118 T13094 T13091 appos ng/ml,TNF-α
R7119 T13095 T13094 punct ),ng/ml
R712 T1559 T1560 nsubj we,investigated
R7120 T13096 T13091 punct ",",TNF-α
R7121 T13097 T13109 nmod IL-18,levels
R7122 T13098 T13100 punct (,ng/ml
R7123 T13099 T13100 nummod 10,ng/ml
R7124 T13100 T13097 appos ng/ml,IL-18
R7125 T13101 T13100 punct ),ng/ml
R7126 T13102 T13097 cc or,IL-18
R7127 T13103 T13097 conj TGF-β,IL-18
R7128 T13104 T13106 punct (,ng/ml
R7129 T13105 T13106 nummod 10,ng/ml
R713 T1560 T1560 ROOT investigated,investigated
R7130 T13106 T13103 appos ng/ml,TGF-β
R7131 T13107 T13106 punct ),ng/ml
R7132 T13108 T13109 det the,levels
R7133 T13109 T13112 nsubj levels,were
R7134 T13110 T13109 prep in,levels
R7135 T13111 T13110 pobj IL-17,in
R7136 T13112 T13068 advcl were,detected
R7137 T13113 T13112 acomp unchanged,were
R7138 T13114 T13112 punct (,were
R7139 T13115 T13112 dobj Fig.,were
R714 T1561 T1562 det the,role
R7140 T13116 T13115 nummod 2a,Fig.
R7141 T13117 T13115 punct ),Fig.
R7142 T13118 T13068 punct .,detected
R7143 T13119 T13120 advmod When,treated
R7144 T13120 T13140 advcl treated,observed
R7145 T13121 T13120 prep with,treated
R7146 T13122 T13121 pobj MCP-1,with
R7147 T13123 T13122 punct (,MCP-1
R7148 T13124 T13125 nummod 10,ng/ml
R7149 T13125 T13122 appos ng/ml,MCP-1
R715 T1562 T1560 dobj role,investigated
R7150 T13126 T13125 punct ),ng/ml
R7151 T13127 T13122 cc or,MCP-1
R7152 T13128 T13122 conj IL-6,MCP-1
R7153 T13129 T13131 punct (,ng/ml
R7154 T13130 T13131 nummod 10,ng/ml
R7155 T13131 T13128 appos ng/ml,IL-6
R7156 T13132 T13131 punct ),ng/ml
R7157 T13133 T13128 punct ",",IL-6
R7158 T13134 T13135 amod significant,upregulations
R7159 T13135 T13122 appos upregulations,MCP-1
R716 T1563 T1562 prep of,role
R7160 T13136 T13135 prep of,upregulations
R7161 T13137 T13138 compound IL-17,proteins
R7162 T13138 T13136 pobj proteins,of
R7163 T13139 T13140 auxpass were,observed
R7164 T13140 T13140 ROOT observed,observed
R7165 T13141 T13143 punct (,±
R7166 T13142 T13143 nummod 62,±
R7167 T13143 T13151 nmod ±,±
R7168 T13144 T13143 nummod 42,±
R7169 T13145 T13143 cc and,±
R717 T1564 T1566 det the,3-kinase
R7170 T13146 T13147 nummod 50,±
R7171 T13147 T13143 conj ±,±
R7172 T13148 T13151 nummod 10,±
R7173 T13149 T13148 cc versus,10
R7174 T13150 T13148 conj 31,10
R7175 T13151 T13156 nmod ±,;
R7176 T13152 T13153 nummod 11,pg/ml
R7177 T13153 T13151 appos pg/ml,±
R7178 T13154 T13153 punct ",",pg/ml
R7179 T13155 T13153 conj respectively,pg/ml
R718 T1565 T1566 compound phosphoinositide,3-kinase
R7180 T13156 T13140 punct ;,observed
R7181 T13157 T13158 compound P,<
R7182 T13158 T13159 compound <,0.05
R7183 T13159 T13156 oprd 0.05,;
R7184 T13160 T13159 punct ),0.05
R7185 T13161 T13140 punct ",",observed
R7186 T13162 T13165 mark whereas,observed
R7187 T13163 T13165 nsubjpass none,observed
R7188 T13164 T13165 auxpass was,observed
R7189 T13165 T13140 advcl observed,observed
R719 T1566 T1563 pobj 3-kinase,of
R7190 T13166 T13165 prep with,observed
R7191 T13167 T13166 pobj IL-8,with
R7192 T13168 T13167 punct (,IL-8
R7193 T13169 T13170 nummod 10,ng/ml
R7194 T13170 T13167 appos ng/ml,IL-8
R7195 T13171 T13167 punct ),IL-8
R7196 T13172 T13165 punct ",",observed
R7197 T13173 T13165 conj MIP-1α,observed
R7198 T13174 T13173 punct (,MIP-1α
R7199 T13175 T13176 nummod 10,ng/ml
R720 T1567 T1566 punct (,3-kinase
R7200 T13176 T13173 appos ng/ml,MIP-1α
R7201 T13177 T13176 punct ),ng/ml
R7202 T13178 T13173 cc or,MIP-1α
R7203 T13179 T13173 conj MIP-1β,MIP-1α
R7204 T13180 T13182 punct (,ng/ml
R7205 T13181 T13182 nummod 10,ng/ml
R7206 T13182 T13179 appos ng/ml,MIP-1β
R7207 T13183 T13179 punct ),MIP-1β
R7208 T13184 T13185 punct (,Fig.
R7209 T13185 T13173 appos Fig.,MIP-1α
R721 T1568 T1566 appos PI3K,3-kinase
R7210 T13186 T13185 nummod 2b,Fig.
R7211 T13187 T13173 punct ),MIP-1α
R7212 T13188 T13140 punct .,observed
R722 T1569 T1566 punct ),3-kinase
R723 T1570 T1571 compound /,Akt
R724 T1571 T1572 compound Akt,pathway
R725 T1572 T1562 conj pathway,role
R726 T1573 T1562 prep in,role
R727 T1574 T1575 det the,regulation
R728 T1575 T1573 pobj regulation,in
R729 T1576 T1575 prep of,regulation
R730 T1577 T1578 amod IL-17,production
R731 T1578 T1576 pobj production,of
R732 T1579 T1578 prep in,production
R733 T1580 T1579 pobj RA,in
R734 T1581 T1560 punct .,investigated
R735 T1582 T1585 amod Peripheral,cells
R736 T1583 T1585 compound blood,cells
R737 T1584 T1585 compound mononuclear,cells
R738 T1585 T1599 nsubjpass cells,separated
R739 T1586 T1585 punct (,cells
R740 T1587 T1585 appos PBMC,cells
R741 T1588 T1585 punct ),cells
R742 T1589 T1585 prep from,cells
R743 T1590 T1589 pobj patients,from
R744 T1591 T1585 prep with,cells
R745 T1592 T1591 pobj RA,with
R746 T1593 T1592 punct (,RA
R747 T1594 T1596 nmod n,24
R748 T1595 T1596 punct =,24
R749 T1596 T1592 appos 24,RA
R750 T1597 T1592 punct ),RA
R751 T1598 T1599 auxpass were,separated
R752 T1599 T1599 ROOT separated,separated
R753 T1600 T1599 punct ",",separated
R754 T1601 T1602 advmod then,stimulated
R755 T1602 T1599 conj stimulated,separated
R756 T1603 T1602 prep with,stimulated
R757 T1604 T1605 amod various,agents
R758 T1605 T1603 pobj agents,with
R759 T1606 T1605 prep including,agents
R760 T1607 T1618 amod anti-CD3,cytokines
R761 T1608 T1607 punct ",",anti-CD3
R762 T1609 T1607 conj anti-CD28,anti-CD3
R763 T1610 T1607 punct ",",anti-CD3
R7635 T14019 T14031 nsubj Inhibition,observed
R7636 T14020 T14019 prep of,Inhibition
R7637 T14021 T14022 compound IL-17,production
R7638 T14022 T14020 pobj production,of
R7639 T14023 T14022 prep by,production
R764 T1611 T1607 conj phytohemagglutinin,anti-CD3
R7640 T14024 T14026 nmod signal,inhibitors
R7641 T14025 T14026 compound transduction,inhibitors
R7642 T14026 T14023 pobj inhibitors,by
R7643 T14027 T14026 cc and,inhibitors
R7644 T14028 T14029 amod anti-rheumatic,drugs
R7645 T14029 T14026 conj drugs,inhibitors
R7646 T14030 T14031 aux Having,observed
R7647 T14031 T14041 ccomp observed,was
R7648 T14032 T14035 det the,production
R7649 T14033 T14035 amod increased,production
R765 T1612 T1611 punct (,phytohemagglutinin
R7650 T14034 T14035 compound IL-17,production
R7651 T14035 T14031 dobj production,observed
R7652 T14036 T14035 prep in,production
R7653 T14037 T14038 compound RA,PBMC
R7654 T14038 T14036 pobj PBMC,in
R7655 T14039 T14041 punct ",",was
R7656 T14040 T14041 nsubj it,was
R7657 T14041 T14041 ROOT was,was
R7658 T14042 T14041 acomp important,was
R7659 T14043 T14044 aux to,know
R766 T1613 T1611 appos PHA,phytohemagglutinin
R7660 T14044 T14041 xcomp know,was
R7661 T14045 T14046 nsubj which,signal
R7662 T14046 T14048 nmod signal,pathways
R7663 T14047 T14048 compound transduction,pathways
R7664 T14048 T14050 nsubjpass pathways,involved
R7665 T14049 T14050 auxpass were,involved
R7666 T14050 T14044 ccomp involved,know
R7667 T14051 T14041 punct .,was
R7668 T14052 T14053 mark As,illustrated
R7669 T14053 T14066 advcl illustrated,observed
R767 T1614 T1611 punct ),phytohemagglutinin
R7670 T14054 T14053 prep in,illustrated
R7671 T14055 T14054 pobj Fig.,in
R7672 T14056 T14055 nummod 3,Fig.
R7673 T14057 T14055 punct ",",Fig.
R7674 T14058 T14060 det an,decrease
R7675 T14059 T14060 amod significant,decrease
R7676 T14060 T14066 nsubjpass decrease,observed
R7677 T14061 T14060 prep in,decrease
R7678 T14062 T14064 amod anti-CD3-induced,production
R7679 T14063 T14064 compound IL-17,production
R768 T1615 T1611 cc and,phytohemagglutinin
R7680 T14064 T14061 pobj production,in
R7681 T14065 T14066 auxpass was,observed
R7682 T14066 T14066 ROOT observed,observed
R7683 T14067 T14068 advmod when,co-incubated
R7684 T14068 T14066 advcl co-incubated,observed
R7685 T14069 T14068 prep with,co-incubated
R7686 T14070 T14071 amod NF-κB,inhibitor
R7687 T14071 T14069 pobj inhibitor,with
R7688 T14072 T14071 punct ",",inhibitor
R7689 T14073 T14071 conj PDTC,inhibitor
R769 T1616 T1618 amod several,cytokines
R7690 T14074 T14073 cc and,PDTC
R7691 T14075 T14073 conj dexamethasone,PDTC
R7692 T14076 T14068 prep in,co-incubated
R7693 T14077 T14076 pobj comparison,in
R7694 T14078 T14077 prep with,comparison
R7695 T14079 T14078 pobj anti-CD3,with
R7696 T14080 T14079 advmod alone,anti-CD3
R7697 T14081 T14083 punct (,±
R7698 T14082 T14083 nummod 38,±
R7699 T14083 T14091 nmod ±,±
R770 T1617 T1618 amod inflammatory,cytokines
R7700 T14084 T14083 nummod 5,±
R7701 T14085 T14083 cc and,±
R7702 T14086 T14087 nummod 54,±
R7703 T14087 T14083 conj ±,±
R7704 T14088 T14091 nummod 11,±
R7705 T14089 T14088 cc versus,11
R7706 T14090 T14088 conj 98,11
R7707 T14091 T14079 dep ±,anti-CD3
R7708 T14092 T14093 nummod 19,pg/ml
R7709 T14093 T14091 appos pg/ml,±
R771 T1618 T1606 pobj cytokines,including
R7710 T14094 T14093 punct ",",pg/ml
R7711 T14095 T14091 conj respectively,±
R7712 T14096 T14099 punct ;,0.05
R7713 T14097 T14098 compound P,<
R7714 T14098 T14099 compound <,0.05
R7715 T14099 T14066 npadvmod 0.05,observed
R7716 T14100 T14099 punct ),0.05
R7717 T14101 T14066 punct .,observed
R7718 T14102 T14104 nummod LY294002,wortmannin
R7719 T14103 T14102 cc and,LY294002
R772 T1619 T1618 cc and,cytokines
R7720 T14104 T14114 npadvmod wortmannin,inhibited
R7721 T14105 T14104 punct ",",wortmannin
R7722 T14106 T14114 prep as,inhibited
R7723 T14107 T14108 det an,inhibitor
R7724 T14108 T14106 pobj inhibitor,as
R7725 T14109 T14108 prep of,inhibitor
R7726 T14110 T14109 pobj PI3K,of
R7727 T14111 T14114 punct ",",inhibited
R7728 T14112 T14114 advmod also,inhibited
R7729 T14113 T14114 advmod markedly,inhibited
R773 T1620 T1618 conj chemokines,cytokines
R7730 T14114 T14114 ROOT inhibited,inhibited
R7731 T14115 T14118 det the,production
R7732 T14116 T14118 amod anti-CD3-induced,production
R7733 T14117 T14118 compound IL-17,production
R7734 T14118 T14114 dobj production,inhibited
R7735 T14119 T14118 prep in,production
R7736 T14120 T14121 compound RA,PBMC
R7737 T14121 T14119 pobj PBMC,in
R7738 T14122 T14124 punct (,±
R7739 T14123 T14124 nummod 98,±
R774 T1621 T1599 punct .,separated
R7740 T14124 T14121 appos ±,PBMC
R7741 T14125 T14132 nummod 19,P
R7742 T14126 T14125 cc versus,19
R7743 T14127 T14129 quantmod 38,10
R7744 T14128 T14129 quantmod ±,10
R7745 T14129 T14125 conj 10,19
R7746 T14130 T14129 quantmod pg/ml,10
R7747 T14131 T14132 compound [,P
R7748 T14132 T14133 nsubj P,<
R7749 T14133 T14114 conj <,inhibited
R775 T1622 T1623 compound IL-17,levels
R7750 T14134 T14135 nummod 0.005,]
R7751 T14135 T14133 dobj ],<
R7752 T14136 T14135 cc and,]
R7753 T14137 T14142 nummod 48,P
R7754 T14138 T14142 nmod ±,P
R7755 T14139 T14138 nummod 4,±
R7756 T14140 T14142 compound pg/ml,P
R7757 T14141 T14142 compound [,P
R7758 T14142 T14135 conj P,]
R7759 T14143 T14133 conj <,<
R776 T1623 T1625 nsubjpass levels,determined
R7760 T14144 T14145 nummod 0.05,]
R7761 T14145 T14143 dobj ],<
R7762 T14146 T14145 punct ",",]
R7763 T14147 T14145 npadvmod respectively,]
R7764 T14148 T14145 punct ),]
R7765 T14149 T14133 punct .,<
R7766 T14150 T14152 det The,inhibitors
R7767 T14151 T14152 compound calcineurin,inhibitors
R7768 T14152 T14153 nsubj inhibitors,cyclosporin
R7769 T14153 T14153 ROOT cyclosporin,cyclosporin
R777 T1624 T1625 auxpass were,determined
R7770 T14154 T14153 dobj A,cyclosporin
R7771 T14155 T14154 cc and,A
R7772 T14156 T14158 nsubj FK506,downregulated
R7773 T14157 T14158 advmod also,downregulated
R7774 T14158 T14153 ccomp downregulated,cyclosporin
R7775 T14159 T14161 det the,secretion
R7776 T14160 T14161 compound IL-17,secretion
R7777 T14161 T14158 dobj secretion,downregulated
R7778 T14162 T14164 advmod as,as
R7779 T14163 T14164 advmod well,as
R778 T1625 T1625 ROOT determined,determined
R7780 T14164 T14161 cc as,secretion
R7781 T14165 T14167 det the,protein
R7782 T14166 T14167 amod mitogen-activated,protein
R7783 T14167 T14168 compound protein,kinase
R7784 T14168 T14161 conj kinase,secretion
R7785 T14169 T14170 punct (,MAPK
R7786 T14170 T14168 appos MAPK,kinase
R7787 T14171 T14170 punct ),MAPK
R7788 T14172 T14175 nummod p38,did
R7789 T14173 T14175 nsubj inhibitor,did
R779 T1626 T1625 agent by,determined
R7790 T14174 T14173 nummod SB203580,inhibitor
R7791 T14175 T14158 conj did,downregulated
R7792 T14176 T14175 punct ",",did
R7793 T14177 T14181 mark whereas,had
R7794 T14178 T14181 nsubj rapamycin,had
R7795 T14179 T14178 cc and,rapamycin
R7796 T14180 T14178 conj PD98059,rapamycin
R7797 T14181 T14175 advcl had,did
R7798 T14182 T14183 det no,effect
R7799 T14183 T14181 dobj effect,had
R780 T1627 T1630 nmod sandwich,assay
R7800 T14184 T14183 prep on,effect
R7801 T14185 T14186 compound IL-17,levels
R7802 T14186 T14184 pobj levels,on
R7803 T14187 T14188 punct (,Fig.
R7804 T14188 T14186 appos Fig.,levels
R7805 T14189 T14188 nummod 3,Fig.
R7806 T14190 T14188 punct ),Fig.
R7807 T14191 T14153 punct .,cyclosporin
R7808 T14192 T14193 aux To,evaluate
R7809 T14193 T14207 advcl evaluate,observed
R781 T1628 T1630 nmod enzyme-linked,assay
R7810 T14194 T14195 det the,possibility
R7811 T14195 T14193 dobj possibility,evaluate
R7812 T14196 T14195 prep of,possibility
R7813 T14197 T14198 amod non-specific,inhibition
R7814 T14198 T14196 pobj inhibition,of
R7815 T14199 T14193 prep by,evaluate
R7816 T14200 T14201 det the,drug
R7817 T14201 T14199 pobj drug,by
R7818 T14202 T14193 prep at,evaluate
R7819 T14203 T14204 amod high,concentrations
R782 T1629 T1630 compound immunosorbent,assay
R7820 T14204 T14202 pobj concentrations,at
R7821 T14205 T14207 punct ",",observed
R7822 T14206 T14207 nsubj we,observed
R7823 T14207 T14207 ROOT observed,observed
R7824 T14208 T14210 det the,response
R7825 T14209 T14210 compound dose,response
R7826 T14210 T14207 dobj response,observed
R7827 T14211 T14210 prep of,response
R7828 T14212 T14211 pobj PDTC,of
R7829 T14213 T14212 cc and,PDTC
R783 T1630 T1626 pobj assay,by
R7830 T14214 T14212 conj LY294002,PDTC
R7831 T14215 T14210 prep for,response
R7832 T14216 T14217 det the,inhibition
R7833 T14217 T14215 pobj inhibition,for
R7834 T14218 T14217 prep of,inhibition
R7835 T14219 T14220 amod IL-17,production
R7836 T14220 T14218 pobj production,of
R7837 T14221 T14220 prep in,production
R7838 T14222 T14221 pobj PBMC,in
R7839 T14223 T14207 punct .,observed
R784 T1631 T1630 cc and,assay
R7840 T14224 T14225 expl There,were
R7841 T14225 T14225 ROOT were,were
R7842 T14226 T14227 amod dose-dependent,inhibitions
R7843 T14227 T14225 attr inhibitions,were
R7844 T14228 T14227 prep of,inhibitions
R7845 T14229 T14230 compound IL-17,production
R7846 T14230 T14228 pobj production,of
R7847 T14231 T14227 prep with,inhibitions
R7848 T14232 T14233 compound chemical,inhibitors
R7849 T14233 T14231 pobj inhibitors,with
R785 T1632 T1630 conj reverse,assay
R7850 T14234 T14235 punct (,Fig.
R7851 T14235 T14227 appos Fig.,inhibitions
R7852 T14236 T14235 nummod 4a,Fig.
R7853 T14237 T14235 punct ),Fig.
R7854 T14238 T14225 punct .,were
R7855 T14239 T14241 det The,inhibitors
R7856 T14240 T14241 amod other,inhibitors
R7857 T14241 T14248 nsubj inhibitors,showed
R7858 T14242 T14241 prep in,inhibitors
R7859 T14243 T14242 pobj addition,in
R786 T1633 T1632 dobj transcription,reverse
R7860 T14244 T14243 prep to,addition
R7861 T14245 T14244 pobj PDTC,to
R7862 T14246 T14245 cc and,PDTC
R7863 T14247 T14245 conj LY294002,PDTC
R7864 T14248 T14248 ROOT showed,showed
R7865 T14249 T14251 det the,pattern
R7866 T14250 T14251 amod same,pattern
R7867 T14251 T14248 dobj pattern,showed
R7868 T14252 T14251 prep of,pattern
R7869 T14253 T14252 pobj inhibition,of
R787 T1634 T1636 compound polymerase,reaction
R7870 T14254 T14248 punct .,showed
R7871 T14255 T14256 amod Cytotoxic,effects
R7872 T14256 T14266 nsubj effects,were
R7873 T14257 T14256 prep on,effects
R7874 T14258 T14257 pobj PBMC,on
R7875 T14259 T14256 prep by,effects
R7876 T14260 T14262 det the,inhibitors
R7877 T14261 T14262 compound chemical,inhibitors
R7878 T14262 T14259 pobj inhibitors,by
R7879 T14263 T14262 prep at,inhibitors
R788 T1635 T1636 compound chain,reaction
R7880 T14264 T14265 amod experimental,concentrations
R7881 T14265 T14263 pobj concentrations,at
R7882 T14266 T14266 ROOT were,were
R7883 T14267 T14266 neg not,were
R7884 T14268 T14266 acomp observed,were
R7885 T14269 T14268 punct (,observed
R7886 T14270 T14272 nmod Fig.,)
R7887 T14271 T14270 nummod 4b,Fig.
R7888 T14272 T14268 punct ),observed
R7889 T14273 T14266 punct .,were
R789 T1636 T1630 appos reaction,assay
R790 T1637 T1625 punct .,determined
R791 T1707 T1706 prep of,PBMC
R792 T1638 T1639 det The,production
R793 T1708 T1707 pobj patients,of
R794 T1639 T1644 nsubjpass production,increased
R795 T1709 T1708 prep with,patients
R796 T1710 T1709 pobj osteoarthritis,with
R797 T1711 T1703 punct ",",detected
R798 T1640 T1639 prep of,production
R799 T1712 T1703 cc but,detected
R800 T1713 T1715 poss their,levels
R801 T1714 T1715 compound expression,levels
R802 T1641 T1640 pobj IL-17,of
R803 T1642 T1644 auxpass was,increased
R804 T1643 T1644 advmod significantly,increased
R805 T1644 T1644 ROOT increased,increased
R8059 T14613 T14614 compound IL-17,mRNA
R806 T1645 T1644 prep in,increased
R8060 T14614 T14615 compound mRNA,expression
R8061 T14615 T14615 ROOT expression,expression
R8062 T14616 T14615 prep in,expression
R8063 T14617 T14618 compound RA,PBMC
R8064 T14618 T14616 pobj PBMC,in
R8065 T14619 T14620 aux To,see
R8066 T14620 T14615 advcl see,expression
R8067 T14621 T14627 mark whether,regulated
R8068 T14622 T14624 amod enhanced,production
R8069 T14623 T14624 compound IL-17,production
R807 T1715 T1716 nsubj levels,were
R8070 T14624 T14627 nsubjpass production,regulated
R8071 T14625 T14627 aux could,regulated
R8072 T14626 T14627 auxpass be,regulated
R8073 T14627 T14620 ccomp regulated,see
R8074 T14628 T14627 prep at,regulated
R8075 T14629 T14631 det a,level
R8076 T14630 T14631 amod transcriptional,level
R8077 T14631 T14628 pobj level,at
R8078 T14632 T14635 punct ",",transcription
R8079 T14633 T14635 amod semi-quantatitive,transcription
R808 T1716 T1703 conj were,detected
R8080 T14634 T14635 compound reverse,transcription
R8081 T14635 T14638 compound transcription,reaction
R8082 T14636 T14638 compound polymerase,reaction
R8083 T14637 T14638 compound chain,reaction
R8084 T14638 T14640 nsubjpass reaction,performed
R8085 T14639 T14640 auxpass was,performed
R8086 T14640 T14627 conj performed,regulated
R8087 T14641 T14615 punct .,expression
R8088 T14642 T14643 nsubj We,observed
R8089 T14643 T14643 ROOT observed,observed
R809 T1646 T1645 pobj cells,in
R8090 T14644 T14646 det a,increase
R8091 T14645 T14646 amod dose-dependent,increase
R8092 T14646 T14643 dobj increase,observed
R8093 T14647 T14646 prep in,increase
R8094 T14648 T14649 compound IL-17,mRNA
R8095 T14649 T14650 compound mRNA,transcripts
R8096 T14650 T14647 pobj transcripts,in
R8097 T14651 T14646 prep after,increase
R8098 T14652 T14651 pobj stimulation,after
R8099 T14653 T14652 prep with,stimulation
R810 T1717 T1718 advmod much,lower
R8100 T14654 T14653 pobj anti-CD3,with
R8101 T14655 T14658 punct ;,inhibited
R8102 T14656 T14658 nsubjpass this,inhibited
R8103 T14657 T14658 auxpass was,inhibited
R8104 T14658 T14643 ccomp inhibited,observed
R8105 T14659 T14658 agent by,inhibited
R8106 T14660 T14662 det the,inhibitor
R8107 T14661 T14662 compound PI3K,inhibitor
R8108 T14662 T14659 pobj inhibitor,by
R8109 T14663 T14662 appos LY294002,inhibitor
R811 T1718 T1716 acomp lower,were
R8110 T14664 T14659 cc and,by
R8111 T14665 T14659 conj by,by
R8112 T14666 T14669 det the,PDTC
R8113 T14667 T14669 amod NF-κB,PDTC
R8114 T14668 T14669 compound inhibitor,PDTC
R8115 T14669 T14665 pobj PDTC,by
R8116 T14670 T14671 punct (,Fig.
R8117 T14671 T14669 appos Fig.,PDTC
R8118 T14672 T14671 nummod 5,Fig.
R8119 T14673 T14669 punct ),PDTC
R812 T1719 T1718 prep than,lower
R8120 T14674 T14643 punct .,observed
R813 T1720 T1719 pobj those,than
R814 T1721 T1720 prep of,those
R815 T1722 T1723 compound RA,PBMC
R816 T1723 T1721 pobj PBMC,of
R817 T1647 T1646 acl treated,cells
R818 T1724 T1716 punct .,were
R819 T1725 T1726 amod Anti-CD3,antibody
R820 T1726 T1727 nsubj antibody,activated
R821 T1727 T1727 ROOT activated,activated
R822 T1648 T1647 prep with,treated
R823 T1728 T1730 det the,pathway
R824 T1729 T1730 amod PI3K/Akt,pathway
R825 T1730 T1727 dobj pathway,activated
R826 T1649 T1650 amod anti-CD3,antibody
R827 T1731 T1730 punct ;,pathway
R828 T1732 T1736 nsubj activation,resulted
R829 T1733 T1732 prep of,activation
R830 T1650 T1648 pobj antibody,with
R831 T1734 T1735 det this,pathway
R832 T1735 T1733 pobj pathway,of
R833 T1651 T1648 conj with,with
R834 T1736 T1727 conj resulted,activated
R835 T1737 T1736 prep in,resulted
R836 T1738 T1740 det a,augmentation
R8360 T15245 T15257 nsubj Activation,determine
R8361 T15246 T15245 prep of,Activation
R8362 T15247 T15250 compound PI3K/Akt,pathway
R8363 T15248 T15250 compound signal,pathway
R8364 T15249 T15250 compound transduction,pathway
R8365 T15250 T15246 pobj pathway,of
R8366 T15251 T15250 prep on,pathway
R8367 T15252 T15253 compound IL-17,production
R8368 T15253 T15251 pobj production,on
R8369 T15254 T15245 agent by,Activation
R837 T1739 T1740 amod pronounced,augmentation
R8370 T15255 T15254 pobj anti-CD3,by
R8371 T15256 T15257 aux To,determine
R8372 T15257 T15267 advcl determine,evaluated
R8373 T15258 T15260 amod downstream,molecules
R8374 T15259 T15260 compound effector,molecules
R8375 T15260 T15257 dobj molecules,determine
R8376 T15261 T15260 prep of,molecules
R8377 T15262 T15264 det the,pathway
R8378 T15263 T15264 compound PI3K,pathway
R8379 T15264 T15261 pobj pathway,of
R838 T1652 T1651 cc or,with
R8380 T15265 T15267 punct ",",evaluated
R8381 T15266 T15267 nsubj we,evaluated
R8382 T15267 T15267 ROOT evaluated,evaluated
R8383 T15268 T15269 det the,activation
R8384 T15269 T15267 dobj activation,evaluated
R8385 T15270 T15269 prep of,activation
R8386 T15271 T15270 pobj Akt,of
R8387 T15272 T15269 prep by,activation
R8388 T15273 T15274 amod western,blotting
R8389 T15274 T15272 pobj blotting,by
R839 T1740 T1737 pobj augmentation,in
R8390 T15275 T15267 punct .,evaluated
R8391 T15276 T15277 mark As,shown
R8392 T15277 T15311 advcl shown,observed
R8393 T15278 T15277 prep in,shown
R8394 T15279 T15278 pobj Fig.,in
R8395 T15280 T15279 nummod 6,Fig.
R8396 T15281 T15279 punct ",",Fig.
R8397 T15282 T15277 prep at,shown
R8398 T15283 T15284 nummod 10,min
R8399 T15284 T15282 pobj min,at
R840 T1741 T1740 prep of,augmentation
R8400 T15285 T15284 prep of,min
R8401 T15286 T15285 pobj incubation,of
R8402 T15287 T15284 prep with,min
R8403 T15288 T15287 pobj anti-CD3,with
R8404 T15289 T15288 punct (,anti-CD3
R8405 T15290 T15291 nummod 10,μg
R8406 T15291 T15293 nmod μg,ml
R8407 T15292 T15293 compound /,ml
R8408 T15293 T15288 appos ml,anti-CD3
R8409 T15294 T15293 punct ),ml
R841 T1742 T1743 amod nuclear,factor
R8410 T15295 T15288 cc or,anti-CD3
R8411 T15296 T15288 conj LY294002,anti-CD3
R8412 T15297 T15296 punct (,LY294002
R8413 T15298 T15299 nummod 20,μM
R8414 T15299 T15296 appos μM,LY294002
R8415 T15300 T15296 punct ),LY294002
R8416 T15301 T15284 punct ",",min
R8417 T15302 T15303 det no,difference
R8418 T15303 T15311 nsubjpass difference,observed
R8419 T15304 T15303 prep in,difference
R842 T1653 T1651 conj without,with
R8420 T15305 T15306 det the,amounts
R8421 T15306 T15304 pobj amounts,in
R8422 T15307 T15306 prep of,amounts
R8423 T15308 T15309 compound phosphorylated,Akt
R8424 T15309 T15307 pobj Akt,of
R8425 T15310 T15311 auxpass was,observed
R8426 T15311 T15311 ROOT observed,observed
R8427 T15312 T15311 punct .,observed
R8428 T15313 T15323 advmod However,increased
R8429 T15314 T15323 punct ",",increased
R843 T1743 T1741 pobj factor,of
R8430 T15315 T15323 prep after,increased
R8431 T15316 T15317 nummod 30,min
R8432 T15317 T15315 pobj min,after
R8433 T15318 T15317 prep of,min
R8434 T15319 T15318 pobj incubation,of
R8435 T15320 T15323 punct ",",increased
R8436 T15321 T15322 compound phosphorylated,Akt
R8437 T15322 T15323 nsubj Akt,increased
R8438 T15323 T15323 ROOT increased,increased
R8439 T15324 T15325 punct (,lane
R844 T1744 T1749 nmod κB,activity
R8440 T15325 T15323 dobj lane,increased
R8441 T15326 T15325 nummod 2,lane
R8442 T15327 T15325 punct ),lane
R8443 T15328 T15323 punct ",",increased
R8444 T15329 T15323 cc and,increased
R8445 T15330 T15331 det the,effect
R8446 T15331 T15340 nsubj effect,reached
R8447 T15332 T15331 prep of,effect
R8448 T15333 T15332 pobj inhibition,of
R8449 T15334 T15331 prep by,effect
R845 T1745 T1746 punct (,NF-κB
R8450 T15335 T15334 pobj LY294002,by
R8451 T15336 T15337 punct (,lane
R8452 T15337 T15335 appos lane,LY294002
R8453 T15338 T15337 nummod 3,lane
R8454 T15339 T15337 punct ),lane
R8455 T15340 T15323 conj reached,increased
R8456 T15341 T15342 det a,peak
R8457 T15342 T15340 dobj peak,reached
R8458 T15343 T15340 prep at,reached
R8459 T15344 T15345 nummod 60,min
R846 T1654 T1653 pobj anti-CD28,without
R8460 T15345 T15343 pobj min,at
R8461 T15346 T15340 punct ",",reached
R8462 T15347 T15340 advcl lasting,reached
R8463 T15348 T15347 prep to,lasting
R8464 T15349 T15350 quantmod 120,240
R8465 T15350 T15351 nummod 240,min
R8466 T15351 T15348 pobj min,to
R8467 T15352 T15340 punct .,reached
R8468 T15353 T15360 prep In,remained
R8469 T15354 T15353 pobj contrast,In
R847 T1746 T1744 appos NF-κB,κB
R8470 T15355 T15360 punct ",",remained
R8471 T15356 T15357 compound non-phosphorylated,Akt
R8472 T15357 T15360 nsubj Akt,remained
R8473 T15358 T15357 cc and,Akt
R8474 T15359 T15357 conj β-actin,Akt
R8475 T15360 T15360 ROOT remained,remained
R8476 T15361 T15360 acomp unchanged,remained
R8477 T15362 T15360 advmod regardless,remained
R8478 T15363 T15362 prep of,regardless
R8479 T15364 T15365 amod incubation,time
R848 T1747 T1749 punct ),activity
R8480 T15365 T15363 pobj time,of
R8481 T15366 T15360 punct .,remained
R8482 T15367 T15374 nsubj PHA,demonstrated
R8483 T15368 T15367 punct ",",PHA
R8484 T15369 T15370 compound concanavalin,A
R8485 T15370 T15367 conj A,PHA
R8486 T15371 T15370 cc and,A
R8487 T15372 T15374 nsubj IL-15,demonstrated
R8488 T15373 T15374 advmod also,demonstrated
R8489 T15374 T15374 ROOT demonstrated,demonstrated
R849 T1748 T1749 amod DNA-binding,activity
R8490 T15375 T15377 det the,effect
R8491 T15376 T15377 amod same,effect
R8492 T15377 T15374 dobj effect,demonstrated
R8493 T15378 T15377 prep on,effect
R8494 T15379 T15380 compound phosphorylated,Akt
R8495 T15380 T15378 pobj Akt,on
R8496 T15381 T15382 mark as,shown
R8497 T15382 T15374 advcl shown,demonstrated
R8498 T15383 T15382 prep with,shown
R8499 T15384 T15383 pobj anti-CD3,with
R850 T1655 T1654 cc and,anti-CD28
R8500 T15385 T15384 punct ",",anti-CD3
R8501 T15386 T15387 nsubj which,was
R8502 T15387 T15374 ccomp was,demonstrated
R8503 T15388 T15389 det an,inhibition
R8504 T15389 T15387 attr inhibition,was
R8505 T15390 T15389 prep by,inhibition
R8506 T15391 T15390 pobj wortmannin,by
R8507 T15392 T15391 cc and,wortmannin
R8508 T15393 T15391 conj PDTC,wortmannin
R8509 T15394 T15396 advmod as,as
R851 T1749 T1740 appos activity,augmentation
R8510 T15395 T15396 advmod well,as
R8511 T15396 T15390 cc as,by
R8512 T15397 T15390 conj by,by
R8513 T15398 T15397 pobj LY294002,by
R8514 T15399 T15402 punct (,shown
R8515 T15400 T15402 nsubjpass data,shown
R8516 T15401 T15402 neg not,shown
R8517 T15402 T15387 parataxis shown,was
R8518 T15403 T15402 punct ),shown
R8519 T15404 T15374 punct .,demonstrated
R852 T1750 T1736 punct .,resulted
R853 T1751 T1752 compound IL-17,production
R854 T1656 T1654 conj PHA,anti-CD28
R855 T1752 T1757 nsubj production,is
R856 T1753 T1752 prep by,production
R857 T1657 T1656 punct (,PHA
R858 T1754 T1756 amod activated,PBMC
R859 T1755 T1756 compound RA,PBMC
R860 T1756 T1753 pobj PBMC,by
R861 T1757 T1761 auxpass is,blocked
R862 T1758 T1761 advmod completely,blocked
R863 T1759 T1758 cc or,completely
R864 T1760 T1758 conj partly,completely
R865 T1658 T1656 nmod P,PHA
R866 T1761 T1761 ROOT blocked,blocked
R867 T1762 T1761 prep in,blocked
R868 T1763 T1764 det the,presence
R869 T1764 T1762 pobj presence,in
R870 T1659 T1658 nummod <,P
R871 T1765 T1764 prep of,presence
R872 T1766 T1768 det the,inhibitor
R873 T1767 T1768 compound NF-κB,inhibitor
R874 T1660 T1658 nummod 0.05,P
R875 T1768 T1765 pobj inhibitor,of
R876 T1769 T1770 compound pyrrolidine,dithiocarbamate
R877 T1770 T1764 appos dithiocarbamate,presence
R878 T1661 T1656 punct ),PHA
R879 T1771 T1770 cc and,dithiocarbamate
R880 T1772 T1775 det the,wortmannin
R881 T1773 T1775 amod PI3K/Akt,wortmannin
R882 T1662 T1644 punct .,increased
R883 T1774 T1775 compound inhibitor,wortmannin
R884 T1663 T1676 prep Among,upregulated
R885 T1775 T1770 conj wortmannin,dithiocarbamate
R886 T1776 T1775 cc and,wortmannin
R887 T1777 T1775 conj LY294002,wortmannin
R888 T1778 T1777 punct ",",LY294002
R8888 T16398 T16415 nsubj Activation,investigate
R8889 T16399 T16398 prep of,Activation
R889 T1664 T1665 amod tested,cytokines
R8890 T16400 T16401 det the,NF-κB
R8891 T16401 T16399 pobj NF-κB,of
R8892 T16402 T16401 cc and,NF-κB
R8893 T16403 T16404 amod activator,protein-1
R8894 T16404 T16401 conj protein-1,NF-κB
R8895 T16405 T16406 punct (,AP-1
R8896 T16406 T16404 appos AP-1,protein-1
R8897 T16407 T16406 punct ),AP-1
R8898 T16408 T16398 conj pathway,Activation
R8899 T16409 T16398 prep in,Activation
R890 T1779 T1770 conj respectively,dithiocarbamate
R8900 T16410 T16413 det the,region
R8901 T16411 T16413 compound IL-17,region
R8902 T16412 T16413 compound promoter,region
R8903 T16413 T16409 pobj region,in
R8904 T16414 T16415 aux To,investigate
R8905 T16415 T16442 advcl investigate,performed
R8906 T16416 T16415 advmod further,investigate
R8907 T16417 T16420 det the,pathway
R8908 T16418 T16420 amod intracellular,pathway
R8909 T16419 T16420 amod signaling,pathway
R891 T1780 T1761 punct .,blocked
R8910 T16420 T16415 dobj pathway,investigate
R8911 T16421 T16420 acl activated,pathway
R8912 T16422 T16421 agent by,activated
R8913 T16423 T16430 amod anti-CD3,PHA
R8914 T16424 T16423 cc plus,anti-CD3
R8915 T16425 T16423 conj anti-CD28,anti-CD3
R8916 T16426 T16425 punct ",",anti-CD28
R8917 T16427 T16428 compound concanavalin,A
R8918 T16428 T16425 conj A,anti-CD28
R8919 T16429 T16428 punct ",",A
R892 T1781 T1794 advmod However,affect
R8920 T16430 T16422 pobj PHA,by
R8921 T16431 T16430 cc and,PHA
R8922 T16432 T16430 conj IL-15,PHA
R8923 T16433 T16432 punct ",",IL-15
R8924 T16434 T16421 cc and,activated
R8925 T16435 T16421 conj responsible,activated
R8926 T16436 T16435 prep for,responsible
R8927 T16437 T16436 pcomp inducing,for
R8928 T16438 T16439 compound IL-17,expression
R8929 T16439 T16437 dobj expression,inducing
R893 T1665 T1663 pobj cytokines,Among
R8930 T16440 T16442 punct ",",performed
R8931 T16441 T16442 nsubj we,performed
R8932 T16442 T16442 ROOT performed,performed
R8933 T16443 T16446 det an,assay
R8934 T16444 T16446 amod electrophoretic,assay
R8935 T16445 T16446 compound mobility-shift,assay
R8936 T16446 T16442 dobj assay,performed
R8937 T16447 T16446 punct (,assay
R8938 T16448 T16446 appos EMSA,assay
R8939 T16449 T16446 punct ),assay
R894 T1782 T1794 punct ",",affect
R8940 T16450 T16446 prep of,assay
R8941 T16451 T16453 compound NF-κB,sites
R8942 T16452 T16453 compound recognition,sites
R8943 T16453 T16450 pobj sites,of
R8944 T16454 T16453 prep in,sites
R8945 T16455 T16456 det the,promoters
R8946 T16456 T16454 pobj promoters,in
R8947 T16457 T16456 prep of,promoters
R8948 T16458 T16457 pobj IL-17,of
R8949 T16459 T16442 punct .,performed
R895 T1783 T1794 nsubj inhibition,affect
R8950 T16460 T16461 mark As,shown
R8951 T16461 T16471 advcl shown,stimulated
R8952 T16462 T16461 prep in,shown
R8953 T16463 T16462 pobj Fig.,in
R8954 T16464 T16463 nummod 7a,Fig.
R8955 T16465 T16463 punct ",",Fig.
R8956 T16466 T16467 amod nuclear,extracts
R8957 T16467 T16471 nsubj extracts,stimulated
R8958 T16468 T16467 prep from,extracts
R8959 T16469 T16470 compound RA,PBMC
R896 T1784 T1783 prep of,inhibition
R8960 T16470 T16468 pobj PBMC,from
R8961 T16471 T16471 ROOT stimulated,stimulated
R8962 T16472 T16471 prep with,stimulated
R8963 T16473 T16472 pobj anti-CD3,with
R8964 T16474 T16473 cc plus,anti-CD3
R8965 T16475 T16473 conj anti-CD28,anti-CD3
R8966 T16476 T16477 punct (,lane
R8967 T16477 T16473 appos lane,anti-CD3
R8968 T16478 T16477 nummod 2,lane
R8969 T16479 T16471 punct ),stimulated
R897 T1666 T1665 cc and,cytokines
R8970 T16480 T16471 conj demonstrated,stimulated
R8971 T16481 T16482 amod increased,binding
R8972 T16482 T16480 dobj binding,demonstrated
R8973 T16483 T16482 prep of,binding
R8974 T16484 T16483 pobj NF-κB,of
R8975 T16485 T16484 prep to,NF-κB
R8976 T16486 T16487 compound IL-17,promoters
R8977 T16487 T16485 pobj promoters,to
R8978 T16488 T16480 prep in,demonstrated
R8979 T16489 T16488 pobj comparison,in
R898 T1785 T1786 amod activator,protein-1
R8980 T16490 T16489 prep with,comparison
R8981 T16491 T16490 pobj that,with
R8982 T16492 T16491 prep of,that
R8983 T16493 T16492 pobj controls,of
R8984 T16494 T16495 punct (,lane
R8985 T16495 T16493 parataxis lane,controls
R8986 T16496 T16495 nummod 1,lane
R8987 T16497 T16495 punct ),lane
R8988 T16498 T16471 punct .,stimulated
R8989 T16499 T16501 det A,assay
R899 T1786 T1784 pobj protein-1,of
R8990 T16500 T16501 compound supershift,assay
R8991 T16501 T16502 nsubj assay,demonstrated
R8992 T16502 T16502 ROOT demonstrated,demonstrated
R8993 T16503 T16504 amod shifted,bands
R8994 T16504 T16502 dobj bands,demonstrated
R8995 T16505 T16502 prep in,demonstrated
R8996 T16506 T16510 nummod p65,lanes
R8997 T16507 T16506 cc and,p65
R8998 T16508 T16506 conj p50,p65
R8999 T16509 T16510 punct (,lanes
R900 T1787 T1786 cc and,protein-1
R9000 T16510 T16505 pobj lanes,in
R9001 T16511 T16510 nummod 3,lanes
R9002 T16512 T16511 cc and,3
R9003 T16513 T16511 conj 4,3
R9004 T16514 T16511 punct ),3
R9005 T16515 T16510 neg not,lanes
R9006 T16516 T16510 prep in,lanes
R9007 T16517 T16516 pobj c-Rel,in
R9008 T16518 T16519 punct (,lane
R9009 T16519 T16517 appos lane,c-Rel
R901 T1667 T1665 conj chemokines,cytokines
R9010 T16520 T16519 nummod 5,lane
R9011 T16521 T16519 punct ),lane
R9012 T16522 T16502 punct .,demonstrated
R9013 T16523 T16530 prep In,observed
R9014 T16524 T16523 pobj normal,In
R9015 T16525 T16530 nsubjpass PBMC,observed
R9016 T16526 T16528 det the,pattern
R9017 T16527 T16528 amod same,pattern
R9018 T16528 T16525 appos pattern,PBMC
R9019 T16529 T16530 auxpass was,observed
R902 T1788 T1791 nmod extracellular,1/2
R9020 T16530 T16530 ROOT observed,observed
R9021 T16531 T16530 punct ",",observed
R9022 T16532 T16530 cc but,observed
R9023 T16533 T16534 det the,degree
R9024 T16534 T16542 nsubj degree,was
R9025 T16535 T16534 prep of,degree
R9026 T16536 T16537 amod NF-κB,activation
R9027 T16537 T16535 pobj activation,of
R9028 T16538 T16537 prep by,activation
R9029 T16539 T16542 nsubj anti-CD3,was
R903 T1789 T1791 amod signal-regulated,1/2
R9030 T16540 T16539 cc plus,anti-CD3
R9031 T16541 T16539 conj anti-CD28,anti-CD3
R9032 T16542 T16530 conj was,observed
R9033 T16543 T16544 advmod less,intense
R9034 T16544 T16542 acomp intense,was
R9035 T16545 T16544 prep than,intense
R9036 T16546 T16545 pobj that,than
R9037 T16547 T16546 prep in,that
R9038 T16548 T16549 compound RA,PBMC
R9039 T16549 T16547 pobj PBMC,in
R904 T1790 T1791 compound kinase,1/2
R9040 T16550 T16549 punct (,PBMC
R9041 T16551 T16549 appos Fig.,PBMC
R9042 T16552 T16551 nummod 7b,Fig.
R9043 T16553 T16549 punct ),PBMC
R9044 T16554 T16542 punct .,was
R9045 T16555 T16556 aux To,confirm
R9046 T16556 T16566 advcl confirm,performed
R9047 T16557 T16558 det the,link
R9048 T16558 T16556 dobj link,confirm
R9049 T16559 T16558 prep between,link
R905 T1668 T1667 punct ",",chemokines
R9050 T16560 T16561 amod PI3K,activity
R9051 T16561 T16559 pobj activity,between
R9052 T16562 T16561 cc and,activity
R9053 T16563 T16561 conj NF-κB,activity
R9054 T16564 T16566 punct ",",performed
R9055 T16565 T16566 nsubj we,performed
R9056 T16566 T16566 ROOT performed,performed
R9057 T16567 T16566 dobj EMSA,performed
R9058 T16568 T16569 aux to,determine
R9059 T16569 T16566 advcl determine,performed
R906 T1791 T1786 conj 1/2,protein-1
R9060 T16570 T16573 det the,activity
R9061 T16571 T16573 amod NF-κB,activity
R9062 T16572 T16573 amod binding,activity
R9063 T16573 T16569 dobj activity,determine
R9064 T16574 T16569 prep after,determine
R9065 T16575 T16574 pobj treatment,after
R9066 T16576 T16575 prep with,treatment
R9067 T16577 T16578 det both,LY294002
R9068 T16578 T16576 pobj LY294002,with
R9069 T16579 T16578 cc and,LY294002
R907 T1792 T1794 aux did,affect
R9070 T16580 T16578 conj PDTC,LY294002
R9071 T16581 T16566 punct .,performed
R9072 T16582 T16583 preconj Both,agents
R9073 T16583 T16584 nsubj agents,block
R9074 T16584 T16584 ROOT block,block
R9075 T16585 T16587 nmod NF-κB,activity
R9076 T16586 T16587 amod DNA-binding,activity
R9077 T16587 T16584 dobj activity,block
R9078 T16588 T16587 prep in,activity
R9079 T16589 T16591 det the,promoter
R908 T1793 T1794 neg not,affect
R9080 T16590 T16591 compound IL-17,promoter
R9081 T16591 T16588 pobj promoter,in
R9082 T16592 T16584 punct (,block
R9083 T16593 T16584 npadvmod Fig.,block
R9084 T16594 T16593 nummod 7c,Fig.
R9085 T16595 T16584 punct ),block
R9086 T16596 T16584 punct .,block
R9087 T16597 T16598 amod Western,blotting
R9088 T16598 T16601 nsubj blotting,showed
R9089 T16599 T16598 prep for,blotting
R909 T1669 T1676 nsubj IL-15,upregulated
R9090 T16600 T16601 nsubj IκB-α,showed
R9091 T16601 T16601 ROOT showed,showed
R9092 T16602 T16601 dobj inhibition,showed
R9093 T16603 T16602 prep of,inhibition
R9094 T16604 T16603 pobj degradation,of
R9095 T16605 T16604 prep of,degradation
R9096 T16606 T16605 pobj IκB-α,of
R9097 T16607 T16601 prep by,showed
R9098 T16608 T16607 pobj LY294002,by
R9099 T16609 T16608 cc and,LY294002
R910 T1670 T1669 punct ",",IL-15
R9100 T16610 T16608 conj PDTC,LY294002
R9101 T16611 T16601 prep at,showed
R9102 T16612 T16614 det the,time
R9103 T16613 T16614 amod same,time
R9104 T16614 T16611 pobj time,at
R9105 T16615 T16601 punct (,showed
R9106 T16616 T16601 dobj Fig.,showed
R9107 T16617 T16616 nummod 7c,Fig.
R9108 T16618 T16616 punct ),Fig.
R9109 T16619 T16601 punct .,showed
R911 T1671 T1673 nmod monocyte,protein-1
R9110 T16620 T16628 prep In,activated
R9111 T16621 T16620 pobj contrast,In
R9112 T16622 T16628 punct ",",activated
R9113 T16623 T16625 det the,pathway
R9114 T16624 T16625 compound AP-1,pathway
R9115 T16625 T16628 nsubjpass pathway,activated
R9116 T16626 T16628 auxpass was,activated
R9117 T16627 T16628 neg not,activated
R9118 T16628 T16628 ROOT activated,activated
R9119 T16629 T16628 agent by,activated
R912 T1794 T1794 ROOT affect,affect
R9120 T16630 T16629 pobj stimulation,by
R9121 T16631 T16628 prep with,activated
R9122 T16632 T16636 amod anti-CD3,data
R9123 T16633 T16632 cc plus,anti-CD3
R9124 T16634 T16632 conj anti-CD28,anti-CD3
R9125 T16635 T16636 punct (,data
R9126 T16636 T16631 pobj data,with
R9127 T16637 T16638 neg not,shown
R9128 T16638 T16636 acl shown,data
R9129 T16639 T16636 punct ),data
R913 T1795 T1796 amod IL-17,production
R9130 T16640 T16628 punct ",",activated
R9131 T16641 T16628 advcl demonstrating,activated
R9132 T16642 T16644 mark that,is
R9133 T16643 T16644 nsubj NF-κB,is
R9134 T16644 T16641 ccomp is,demonstrating
R9135 T16645 T16649 det the,pathway
R9136 T16646 T16649 amod main,pathway
R9137 T16647 T16649 amod intracellular,pathway
R9138 T16648 T16649 amod signaling,pathway
R9139 T16649 T16644 attr pathway,is
R914 T1796 T1794 dobj production,affect
R9140 T16650 T16649 prep in,pathway
R9141 T16651 T16652 amod IL-17,production
R9142 T16652 T16650 pobj production,in
R9143 T16653 T16649 prep by,pathway
R9144 T16654 T16655 amod activated,PBMC
R9145 T16655 T16653 pobj PBMC,by
R9146 T16656 T16649 prep from,pathway
R9147 T16657 T16656 pobj patients,from
R9148 T16658 T16649 prep with,pathway
R9149 T16659 T16658 pobj RA,with
R915 T1797 T1794 punct .,affect
R9150 T16660 T16628 punct .,activated
R916 T1672 T1673 compound chemoattractant,protein-1
R917 T1798 T1799 det These,results
R918 T1799 T1800 nsubj results,suggest
R919 T1800 T1800 ROOT suggest,suggest
R920 T1673 T1669 appos protein-1,IL-15
R921 T1801 T1811 mark that,involved
R922 T1802 T1811 nsubjpass signal,involved
R923 T1803 T1804 compound transduction,pathways
R924 T1674 T1673 cc and,protein-1
R925 T1804 T1811 nsubjpass pathways,involved
R926 T1805 T1804 amod dependent,pathways
R927 T1806 T1805 prep on,dependent
R928 T1675 T1673 conj IL-6,protein-1
R929 T1807 T1806 pobj PI3K/Akt,on
R930 T1808 T1807 cc and,PI3K/Akt
R931 T1809 T1807 conj NF-κB,PI3K/Akt
R932 T1676 T1676 ROOT upregulated,upregulated
R933 T1810 T1811 auxpass are,involved
R934 T1811 T1800 ccomp involved,suggest
R935 T1812 T1811 prep in,involved
R936 T1677 T1678 amod IL-17,production
R937 T1813 T1814 det the,overproduction
R938 T1678 T1676 dobj production,upregulated
R939 T1679 T1678 punct (,production
R940 T1680 T1678 appos P,production
R941 T1681 T1680 nummod <,P
R942 T1682 T1680 nummod 0.05,P
R943 T1683 T1678 punct ),production
R944 T1814 T1812 pobj overproduction,in
R945 T1815 T1814 prep of,overproduction
R946 T1816 T1820 det the,IL-17
R947 T1684 T1676 punct ",",upregulated
R948 T1817 T1820 amod key,IL-17
R949 T1818 T1819 amod inflammatory,cytokine
R950 T1819 T1820 compound cytokine,IL-17
R951 T1685 T1697 mark whereas,did
R952 T1820 T1815 pobj IL-17,of
R953 T1821 T1820 prep in,IL-17
R954 T1686 T1688 compound tumor,factor-α
R955 T1822 T1821 pobj RA,in
R956 T1823 T1800 punct .,suggest
R957 T1687 T1688 compound necrosis,factor-α
R958 T1688 T1697 nsubj factor-α,did
R959 T1689 T1690 punct ",",IL-1β
R960 T1690 T1688 appos IL-1β,factor-α
R961 T1691 T1690 punct ",",IL-1β
R962 T1692 T1690 conj IL-18,IL-1β
R963 T1693 T1692 cc or,IL-18
R964 T1694 T1692 conj transforming,IL-18
R965 T1695 T1696 compound growth,factor-β
R966 T1696 T1694 dobj factor-β,transforming
R967 T1697 T1676 advcl did,upregulated
R970 T1698 T1697 neg not,did
R971 T1699 T1697 punct .,did
R972 T1700 T1703 nsubjpass IL-17,detected
R974 T1701 T1703 auxpass was,detected
R975 T1702 T1703 advmod also,detected
R976 T1703 T1703 ROOT detected,detected
R977 T1704 T1703 prep in,detected
R980 T1705 T1706 det the,PBMC
R982 T1706 T1704 pobj PBMC,in