BB-rel@ldeleger:BB-rel-351567 JSONTXT

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Id Subject Object Predicate Lexical cue
T1 0-72 Title denotes A cell-free system for the replication fo bacteriophage M-13 duplex DNA.
T3 42-60 Microorganism denotes bacteriophage M-13
T2 73-1055 Paragraph denotes Cell-free extracts from M-13 am5 infected Escherichia coli cells which are highly concentrated on cellophane membrane disks replicate efficiently endogenous M-13 duplex DNA. If the reaction is carried out in the presence of bromodeoxyuridine triphosphate, the majority of the label is found in two classes of hybrid DNA molecules in which either the viral or the complementary strand is newly synthesized. A minor portion of the label is incorporated into fully synthetic duplex DNA. DNA synthesis requires ATP and is inhibited by nalidixic acid, novobiocin, and arabinosylnucleoside triphosphates. Rifampicin blocks preferentially the synthesis of molecules with labeled complementary strands. A similar effect is observed upon addition of the helix-destabilising M-13 gene V protein. In contrast, addition of E. coli helix-destabilising protein (Eco HD-protein) stimulates the synthesis of both types of hybrid DNA molecules as well as the formation of fully synthetic duplex DNA.
T4 97-105 Microorganism denotes M-13 am5
E1 97-105 Lives_In denotes M-13 am5
E2 97-105 Exhibits denotes M-13 am5
T5 102-105 Phenotype denotes am5
T6 115-131 Microorganism denotes Escherichia coli
E3 115-131 Lives_In denotes Escherichia coli
T7 171-196 Habitat denotes cellophane membrane disks
T8 230-234 Microorganism denotes M-13
T9 838-842 Microorganism denotes M-13
T10 884-891 Microorganism denotes E. coli
R1 E1 T4 Microorganism M-13 am5,M-13 am5
R2 E1 T6 Location M-13 am5,Escherichia coli
R3 E2 T4 Microorganism M-13 am5,M-13 am5
R4 E2 T5 Property M-13 am5,am5
R5 E3 T6 Microorganism Escherichia coli,Escherichia coli
R6 E3 T7 Location Escherichia coli,cellophane membrane disks