BB-rel@ldeleger:BB-rel-23902156
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| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-90 | Title | denotes | Isolation, expression and characterization of a minor allergen from Penicillium crustosum. |
| T3 | 68-89 | Microorganism | denotes | Penicillium crustosum |
| T2 | 91-1393 | Paragraph | denotes | A ribosomal P1 protein, Pen b 26 from Penicillium brevicompactum, was previously identified as a major allergen. A homolog protein was isolated and characterized from Penicillium crustosum which is not known to be allergenic mold. A cDNA library of P. crustosum was constructed and screened using a probe based on the DNA sequence of Pen b 26. A positive clone was isolated, expressed in Escherichia coli, purified and characterized by comparing its immunological and physical properties to Pen b 26. It was designated as Pen cr 26 and had a 321 nt ORF corresponding to 107 amino acids with a MW of 11 kDa. Pen cr 26 had strong sequence homology to Pen b 26 (92% for nucleotides and 86% for amino acids) and its physical and predicted structural properties were similar to the latter. The level of expression of Pen cr 26 was much lower than that of Pen b 26 in the same expression vector. Both proteins were recognized equally well by the IgG class specific antibodies, but Pen cr 26 was poorly recognized by Penicillium-sensitive atopic sera (IgE), suggesting striking antigenic difference in IgE epitopes, i.e., 87% were positive for Pen b 26 while only 23% were positive for Pen cr 26. The allergenicity of Pen cr 26 seems to be minor in nature and it could be a hypoallergenic variant of Pen b 26. |
| T4 | 129-155 | Microorganism | denotes | Penicillium brevicompactum |
| T5 | 258-279 | Microorganism | denotes | Penicillium crustosum |
| T6 | 340-352 | Microorganism | denotes | P. crustosum |
| T7 | 479-495 | Microorganism | denotes | Escherichia coli |
| T8 | 1101-1112 | Microorganism | denotes | Penicillium |
| T9 | 1101-1134 | Habitat | denotes | Penicillium-sensitive atopic sera |