BB-norm@ldeleger:BB-norm-18346136
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| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-107 | Title | denotes | Detection of Salmonella enterica in food using two-step enrichment and real-time polymerase chain reaction. |
| T6 | 13-32 | Microorganism | denotes | Salmonella enterica |
| T7 | 36-40 | Habitat | denotes | food |
| T2 | 108-226 | Paragraph | denotes | A new real-time polymerase chain reaction-based method was developed for the detection of Salmonella enterica in food. |
| T8 | 198-217 | Microorganism | denotes | Salmonella enterica |
| T9 | 221-225 | Habitat | denotes | food |
| T3 | 227-1229 | Paragraph | denotes | The method consisted of a novel two-step enrichment involving overnight incubation in buffered peptone water and a 5-h subculture in Rappaport-Vassiliadis medium, lysis of bacterial cells and a Salmonella-specific 5'-nuclease real-time PCR with an exogenous internal amplification control. Because a two-step enrichment was used, the detection limit for dead S. enterica cells in artificially contaminated ice cream and salami samples was high at 10(7 )CFU (25 g)(-1), eliminating potential false-positive results. When the method was evaluated with a range of 100 naturally contaminated food samples, three positive samples were detected by both the real-time PCR-based method and by the standard microbiological method, according to EN ISO 6579. When the real-time PCR-based method was evaluated alongside the standard microbiological method according to EN ISO 6579 with 36 food samples artificially contaminated at a level of 10(0 )CFU (25 g)(-1), identical results were obtained from both methods. |
| T10 | 313-335 | Habitat | denotes | buffered peptone water |
| T11 | 360-388 | Habitat | denotes | Rappaport-Vassiliadis medium |
| T12 | 421-431 | Microorganism | denotes | Salmonella |
| T13 | 586-597 | Microorganism | denotes | S. enterica |
| T15-0 | 607-632 | _FRAGMENT | denotes | artificially contaminated |
| T14 | 607-642 | Habitat | denotes | artificially contaminated ice cream |
| T15-1 | 647-661 | Habitat | denotes | salami samples |
| T16 | 792-827 | Habitat | denotes | naturally contaminated food samples |
| T17 | 1104-1142 | Habitat | denotes | food samples artificially contaminated |
| T4 | 1230-1368 | Paragraph | denotes | The real-time PCR-based method involving a two-step enrichment produced equivalent results to EN ISO 6579 on the day after sample receipt. |
| T5 | 1369-1445 | Paragraph | denotes | The developed method is suitable for rapid detection of S. enterica in food. |
| T18 | 1425-1436 | Microorganism | denotes | S. enterica |
| T19 | 1440-1444 | Habitat | denotes | food |
| C-T15-0 | T15-1 | T15-0 | _lexicallyChainedTo | salami samples,artificially contaminated |