bionlp-ost-19-BB-norm-test  

BB-norm@ldeleger:BB-norm-10639454 JSONTXT

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Id Subject Object Predicate Lexical cue
T1 0-160 Title denotes Lack of a role of cytotoxic necrotizing factor 1 toxin from Escherichia coli in bacterial pathogenicity and host cytokine response in infected germfree piglets.
T3 60-76 Microorganism denotes Escherichia coli
T4 80-103 Phenotype denotes bacterial pathogenicity
T5 134-159 Habitat denotes infected germfree piglets
T2 161-2493 Paragraph denotes Some Escherichia coli strains isolated from intestinal or extraintestinal infections in pigs produce cytotoxic necrotizing factor 1 (CNF1). In order to analyze the role of CNF1 in the pathogenesis of porcine colibacillosis, newborn colostrum-deprived germfree piglets were orally inoculated with a wild-type CNF1-producing strain (M623) or with an isogenic cnf1 mutant (M623DeltaCNF1). The two isogenic strains induced a high mortality with similar lung and serosal inflammatory lesions, indicating that both strains were pathogenic in these piglets. Bacterial counts in various organs of inoculated piglets revealed an intestinal predisposition of M623 and M623DeltaCNF1 strains for the cecum and colon. Extraintestinal organs (lungs, liver, spleen, and kidney) were also colonized by both strains. Similar colonization of intestinal and extraintestinal tissues in animals inoculated with either strain was observed, except in the ileum, where M623 showed a higher colonization than M623DeltaCNF1. Intestinal (ileum and colon), extraintestinal (lung and kidney), and immune (mesenteric lymph nodes and spleen) tissues were sampled at 1 day postinoculation and analyzed for cytokine expression by a reverse transcriptase PCR technique. Inoculation with E. coli M623 induced an enhanced expression of inflammatory cytokines (interleukin-1alpha [IL-1alpha], tumor necrosis factor alpha, and IL-12p40) in the intestinal organs compared to uninoculated piglets or piglets inoculated with nonpathogenic intestinal E. coli 862B, which is also able to colonize the intestinal tract. There was little difference in cytokine transcript levels in the intestinal and extraintestinal organs in piglets inoculated with E. coli strains M623 or M623DeltaCNF1, except in the ileum, where IL-1alpha and IL-8 mRNA levels correlated with bacterial colonization. Expression of regulatory cytokines (gamma interferon and IL-4) was weak in immune tissues from piglets inoculated with M623 or M623DeltaCNF1. Taken together, our data indicate that the CNF1-producing strain, M623, is pathogenic and induces inflammatory cytokine expression in germfree, colostrum-deprived piglets. Nevertheless, in this model, the CNF1 toxin does not appear to be a major factor for pathogenicity or cytokine response, as demonstrated by the use of an isogenic cnf1 mutant.
T6 166-182 Microorganism denotes Escherichia coli
T7 205-215 Habitat denotes intestinal
T8 219-234 Habitat denotes extraintestinal
T9 249-253 Habitat denotes pigs
T10 361-368 Habitat denotes porcine
T11 385-428 Habitat denotes newborn colostrum-deprived germfree piglets
T12 393-402 Habitat denotes colostrum
T13 459-468 Phenotype denotes wild-type
T14 469-483 Phenotype denotes CNF1-producing
T15 492-496 Microorganism denotes M623
T16 509-529 Phenotype denotes isogenic cnf1 mutant
T17 531-544 Microorganism denotes M623DeltaCNF1
T18 610-614 Habitat denotes lung
T19-0 610-614 _FRAGMENT denotes lung
T21 619-626 Habitat denotes serosal
T20 619-647 Habitat denotes serosal inflammatory lesions
T19-1 627-647 Habitat denotes inflammatory lesions
T22 683-693 Phenotype denotes pathogenic
T23 703-710 Habitat denotes piglets
T24 740-768 Habitat denotes organs of inoculated piglets
T25 750-768 Habitat denotes inoculated piglets
T26 781-791 Habitat denotes intestinal
T27 810-814 Microorganism denotes M623
T28 819-832 Microorganism denotes M623DeltaCNF1
T29 849-854 Habitat denotes cecum
T30 859-864 Habitat denotes colon
T31 866-881 Habitat denotes Extraintestinal
T32 866-888 Habitat denotes Extraintestinal organs
T33 890-895 Habitat denotes lungs
T34 897-902 Habitat denotes liver
T35 904-910 Habitat denotes spleen
T36 916-922 Habitat denotes kidney
T38 985-995 Habitat denotes intestinal
T37-0 985-995 _FRAGMENT denotes intestinal
T40 1000-1015 Habitat denotes extraintestinal
T39 1000-1023 Habitat denotes extraintestinal tissues
T37-1 1016-1023 Habitat denotes tissues
T41 1027-1064 Habitat denotes animals inoculated with either strain
T42 1093-1098 Habitat denotes ileum
T43 1106-1110 Microorganism denotes M623
T44 1145-1158 Microorganism denotes M623DeltaCNF1
T46 1160-1170 Habitat denotes Intestinal
T45-0 1160-1170 _FRAGMENT denotes Intestinal
T47 1172-1177 Habitat denotes ileum
T48 1182-1187 Habitat denotes colon
T50 1190-1205 Habitat denotes extraintestinal
T49-0 1190-1205 _FRAGMENT denotes extraintestinal
T51 1207-1211 Habitat denotes lung
T52 1216-1222 Habitat denotes kidney
T53-0 1229-1235 _FRAGMENT denotes immune
T54 1237-1259 Habitat denotes mesenteric lymph nodes
T55 1264-1270 Habitat denotes spleen
T45-1 1272-1279 Habitat denotes tissues
T49-1 1272-1279 Habitat denotes tissues
T53-1 1272-1279 Habitat denotes tissues
T56 1414-1426 Microorganism denotes E. coli M623
T58 1567-1577 Habitat denotes intestinal
T57 1567-1584 Habitat denotes intestinal organs
T59 1597-1617 Habitat denotes uninoculated piglets
T60 1621-1682 Habitat denotes piglets inoculated with nonpathogenic intestinal E. coli 862B
T61 1645-1658 Phenotype denotes nonpathogenic
T62 1659-1669 Habitat denotes intestinal
T63 1670-1682 Microorganism denotes E. coli 862B
T64 1719-1735 Habitat denotes intestinal tract
T65-0 1802-1812 _FRAGMENT denotes intestinal
T66 1817-1839 Habitat denotes extraintestinal organs
T65-1 1833-1839 Habitat denotes organs
T67-0 1843-1866 _FRAGMENT denotes piglets inoculated with
T68 1843-1887 Habitat denotes piglets inoculated with E. coli strains M623
T70-0 1867-1882 _FRAGMENT denotes E. coli strains
T69 1867-1887 Microorganism denotes E. coli strains M623
T67-1 1891-1904 Habitat denotes M623DeltaCNF1
T70-1 1891-1904 Microorganism denotes M623DeltaCNF1
T71 1920-1925 Habitat denotes ileum
T73-0 2079-2122 _FRAGMENT denotes immune tissues from piglets inoculated with
T72 2079-2127 Habitat denotes immune tissues from piglets inoculated with M623
T75-0 2099-2122 _FRAGMENT denotes piglets inoculated with
T74 2099-2127 Habitat denotes piglets inoculated with M623
T76 2123-2127 Microorganism denotes M623
T73-1 2131-2144 Habitat denotes M623DeltaCNF1
T75-1 2131-2144 Habitat denotes M623DeltaCNF1
T77 2131-2144 Microorganism denotes M623DeltaCNF1
T78 2189-2203 Phenotype denotes CNF1-producing
T79 2212-2216 Microorganism denotes M623
T80 2221-2231 Phenotype denotes pathogenic
T81 2280-2316 Habitat denotes germfree, colostrum-deprived piglets
T82 2290-2299 Habitat denotes colostrum
T83 2403-2416 Phenotype denotes pathogenicity
T84 2472-2492 Phenotype denotes isogenic cnf1 mutant
C-T19-0 T19-1 T19-0 _lexicallyChainedTo inflammatory lesions,lung
C-T37-0 T37-1 T37-0 _lexicallyChainedTo tissues,intestinal
C-T45-0 T45-1 T45-0 _lexicallyChainedTo tissues,Intestinal
C-T49-0 T49-1 T49-0 _lexicallyChainedTo tissues,extraintestinal
C-T53-0 T53-1 T53-0 _lexicallyChainedTo tissues,immune
C-T65-0 T65-1 T65-0 _lexicallyChainedTo organs,intestinal
C-T67-0 T67-1 T67-0 _lexicallyChainedTo M623DeltaCNF1,piglets inoculated with
C-T70-0 T70-1 T70-0 _lexicallyChainedTo M623DeltaCNF1,E. coli strains
C-T73-0 T73-1 T73-0 _lexicallyChainedTo M623DeltaCNF1,immune tissues from piglets inoculated with
C-T75-0 T75-1 T75-0 _lexicallyChainedTo M623DeltaCNF1,piglets inoculated with