bionlp-ost-19-BB-norm-ner-dev  

BB-norm+ner@ldeleger:BB-norm+ner-18789156 JSONTXT

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T1 0-173 Title denotes A novel receptor - ligand pathway for entry of Francisella tularensis in monocyte-like THP-1 cells: interaction between surface nucleolin and bacterial elongation factor Tu.
T5 47-69 Microorganism denotes Francisella tularensis 263
T6 73-98 Habitat denotes monocyte-like THP-1 cells OBT:000062|OBT:003001
T7 174-196 Microorganism denotes Francisella tularensis 263
T2 174-689 Paragraph denotes Francisella tularensis, the causative agent of tularemia, is one of the most infectious human bacterial pathogens. It is phagocytosed by immune cells, such as monocytes and macrophages. The precise mechanisms that initiate bacterial uptake have not yet been elucidated. Participation of C3, CR3, class A scavenger receptors and mannose receptor in bacterial uptake have been already reported. However, contribution of an additional, as-yet-unidentified receptor for F. tularensis internalization has been suggested.
T8 202-230 Phenotype denotes causative agent of tularemia OBT:002806
T9 262-267 Habitat denotes human OBT:002488
T10 262-287 Phenotype denotes human bacterial pathogens OBT:002806
T11 311-323 Habitat denotes immune cells OBT:000320
T12 333-342 Habitat denotes monocytes OBT:003001
T13 347-358 Habitat denotes macrophages OBT:002995
T14 640-653 Microorganism denotes F. tularensis 263
T3 690-1808 Paragraph denotes We show here that cell-surface expressed nucleolin is a receptor for Francisella tularensis Live Vaccine Strain (LVS) and promotes LVS binding and infection of human monocyte-like THP-1 cells. The HB-19 pseudopeptide that binds specifically carboxy-terminal RGG domain of nucleolin inhibits LVS binding and infection of monocyte-like THP-1 cells. In a pull-down assay, elongation factor Tu (EF-Tu), a GTP-binding protein involved in protein translation, usually found in cytoplasm, was recovered among LVS bacterial membrane proteins bound on RGG domain of nucleolin. A specific polyclonal murine antibody was raised against recombinant LVS EF-Tu. By fluorescence and electron microscopy experiments, we found that a fraction of EF-Tu could be detected at the bacterial surface. Anti-EF-Tu antibodies reduced LVS binding to monocyte-like THP-1 cells and impaired infection, even in absence of complement and complement receptors. Interaction between EF-Tu and nucleolin was illustrated by two different pull-down assays using recombinant EF-Tu proteins and either RGG domain of nucleolin or cell solubilized nucleolin.
T15 708-712 Habitat denotes cell OBT:000061
T16 759-801 Microorganism denotes Francisella tularensis Live Vaccine Strain 263
T17 803-806 Microorganism denotes LVS 263
T18 821-824 Microorganism denotes LVS 263
T19 825-832 Phenotype denotes binding OBT:000029
T20 850-855 Habitat denotes human OBT:002488
T21 850-881 Habitat denotes human monocyte-like THP-1 cells OBT:000062|OBT:003001
T22 856-881 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T23 981-984 Microorganism denotes LVS 263
T24 985-992 Phenotype denotes binding OBT:000029
T25 1010-1035 Habitat denotes monocyte-like THP-1 cells OBT:000062|OBT:003001
T26 1192-1195 Microorganism denotes LVS 263
T27 1280-1286 Habitat denotes murine OBT:002530
T28 1327-1330 Microorganism denotes LVS 263
T29 1499-1502 Microorganism denotes LVS 263
T30 1503-1510 Phenotype denotes binding OBT:000029
T31 1514-1539 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T32 1781-1785 Habitat denotes cell OBT:000061
T4 1809-2536 Paragraph denotes Altogether, our results demonstrate that the interaction between surface nucleolin and its bacterial ligand EF-Tu plays an important role in Francisella tularensis adhesion and entry process and may therefore facilitate invasion of host tissues. Since phagosomal escape and intra-cytosolic multiplication of LVS in infected monocytes are very similar to those of human pathogenic F. tularensis ssp tularensis, the mechanism of entry into monocyte-like THP-1 cells, involving interaction between EF-Tu and nucleolin, might be similar in the two subspecies. Thus, the use of either nucleolin-specific pseudopeptide HB-19 or recombinant EF-Tu could provide attractive therapeutic approaches for modulating F. tularensis infection.
T33 1950-1972 Microorganism denotes Francisella tularensis 263
T34 1973-1981 Phenotype denotes adhesion OBT:000029
T35 2029-2053 Phenotype denotes invasion of host tissues OBT:002806
T36 2041-2053 Habitat denotes host tissues OBT:000196
T37 2117-2120 Microorganism denotes LVS 263
T38 2124-2142 Habitat denotes infected monocytes OBT:003001
T39 2172-2177 Habitat denotes human OBT:002488
T40 2172-2188 Phenotype denotes human pathogenic OBT:002806
T41 2189-2217 Microorganism denotes F. tularensis ssp tularensis 119856
T42 2247-2272 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T43 2512-2525 Microorganism denotes F. tularensis 263