BB-kb+ner@ldeleger:BB-kb+ner-19049879
Annnotations
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| Id | Subject | Object | Predicate | Lexical cue | NCBI_Taxonomy | OntoBiotope |
|---|---|---|---|---|---|---|
| T1 | 0-88 | Title | denotes | Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena crocea. | ||
| T3 | 45-65 | Habitat | denotes | large yellow croaker | OBT:003264 | |
| T4 | 67-87 | Habitat | denotes | Pseudosciaena crocea | OBT:003264 | |
| T2 | 89-1466 | Paragraph | denotes | Myeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the interleukin-1 receptor and Toll-like receptor-induced activation of nuclear factor-kappaB (NF-kappaB). In this report, the full-length cDNA of MyD88 was cloned from the large yellow croaker, Pseudosciaena crocea. It was of 1574 bp, including a 5'-terminal untranslated region (UTR) of 89 bp, a 3'-terminal UTR of 621bp and an open reading frame (ORF) of 864 bp encoding a polypeptide of 287 amino acids. It contained a typical death domain at the N-terminal and a conservative Toll/IL-1R (TIR) domain structure at the C-terminal. The quantitative real-time reverse transcription PCR analysis revealed a broad expression of MyD88 with the highest expression in the spleen and the weakest expression in the muscle. The expression of MyD88 after challenge with formalin-inactivated Gram-negative bacterium Vibrio parahaemolyticus was tested in blood, spleen and liver. It suggested that the highest expression was in the spleen (p<0.05) with 1.9 times (at 48 h) as much as that in the control and the lowest expression of MyD88 was in the liver (p<0.05) with 0.29 times (at 3h) of that in the control. These results indicated that as a universal key adaptor in the Toll-like receptor pathway in mammals, MyD88 might play an important role in large yellow croaker defense against pathogenic infection. | ||
| T5 | 338-358 | Habitat | denotes | large yellow croaker | OBT:003264 | |
| T6 | 360-380 | Habitat | denotes | Pseudosciaena crocea | OBT:003264 | |
| T7 | 833-839 | Habitat | denotes | spleen | OBT:000890 | |
| T8 | 874-880 | Habitat | denotes | muscle | OBT:001226 | |
| T9 | 948-961 | Phenotype | denotes | Gram-negative | OBT:000648 | |
| T10 | 972-995 | Microorganism | denotes | Vibrio parahaemolyticus | 670 | |
| E1 | 972-995 | Exhibits | denotes | Vibrio parahaemolyticus | ||
| T11 | 1010-1015 | Habitat | denotes | blood | OBT:000521 | |
| T12 | 1017-1023 | Habitat | denotes | spleen | OBT:000890 | |
| T13 | 1028-1033 | Habitat | denotes | liver | OBT:002725 | |
| T14 | 1087-1093 | Habitat | denotes | spleen | OBT:000890 | |
| T15 | 1205-1210 | Habitat | denotes | liver | OBT:002725 | |
| T16 | 1361-1368 | Habitat | denotes | mammals | OBT:001625 | |
| T17 | 1408-1428 | Habitat | denotes | large yellow croaker | OBT:003264 | |
| T18 | 1445-1455 | Phenotype | denotes | pathogenic | OBT:002669 | |
| R1 | E1 | T10 | Microorganism | Vibrio parahaemolyticus,Vibrio parahaemolyticus | ||
| R2 | E1 | T9 | Property | Vibrio parahaemolyticus,Gram-negative |