| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-86 |
DRI_Approach |
denotes |
Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex. |
| T2 |
87-155 |
DRI_Approach |
denotes |
DNA mismatch repair has a key role in maintaining genomic stability. |
| T3 |
156-383 |
DRI_Background |
denotes |
Defects in mismatch repair cause elevated spontaneous mutation rates and increased instability of simple repetitive sequences, while mutations in human mismatch repair genes result in hereditary nonpolyposis colorectal cancers. |
| T4 |
384-459 |
DRI_Approach |
denotes |
Mismatch recognition represents the first critical step of mismatch repair. |
| T5 |
460-606 |
DRI_Background |
denotes |
Genetic and biochemical studies in yeast and humans have indicated a requirement for MSH2-MSH3 and MSH2-MSH6 heterodimers in mismatch recognition. |
| T6 |
607-688 |
DRI_Background |
denotes |
These complexes have, to some extent, overlapping mismatch binding specificities. |
| T7 |
689-809 |
DRI_Background |
denotes |
MLH1 and PMS1 are the other essential components of mismatch repair, but how they function in this process is not known. |
| T8 |
810-943 |
DRI_Approach |
denotes |
We have purified the yeast MLH1-PMS1 heterodimer to near homogeneity, and examined its effect on MSH2-MSH3 binding to DNA mismatches. |
| T9 |
944-1081 |
DRI_Outcome |
denotes |
By itself, the MLH1-PMS1 complex shows no affinity for mismatched DNA, but it greatly enhances the mismatch binding ability of MSH2-MSH3. |
