UseCases_ArguminSci_Discourse  

PubMed:9203583 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-101 DRI_Challenge denotes Mlh1 is unique among mismatch repair proteins in its ability to promote crossing-over during meiosis.
T2 102-225 DRI_Background denotes In eukaryotes, homologs of the bacterial MutS and MutL proteins function in DNA mismatch repair and recombination pathways.
T3 226-288 DRI_Approach denotes The mutL homolog MLH1 is required for nuclear mismatch repair.
T4 289-405 DRI_Background denotes Previously, cytological analysis of MLH1-deficient mice has implied a role for Mlh1 in crossing-over during meiosis.
T5 406-597 DRI_Outcome denotes Here we demonstrate that Saccharomyces cerevisiae diploids containing a deletion of MLH1 have reduced crossing-over in addition to a deficiency in the repair of mismatched DNA during meiosis.
T6 598-622 DRI_Background denotes Absence of either of the
T7 623-644 Token_Label.OUTSIDE denotes meiosis-specific mutS
T8 645-715 DRI_Background denotes homologs Msh4 or Msh5 results in a similar reduction in crossing-over.
T9 716-829 DRI_Approach denotes Analysis of an mlh1 msh4 double mutant suggests that both genes act in the same pathway to promote crossing-over.
T10 830-933 DRI_Approach denotes All genetic markers analyzed in mlh1 mutants display elevated frequencies of non-Mendelian segregation.
T11 934-958 DRI_Approach denotes Most of these events are
T12 959-983 Token_Label.OUTSIDE denotes postmeiotic segregations
T13 984-1023 DRI_Approach denotes that represent unrepaired heteroduplex.
T14 1024-1142 DRI_Background denotes These data suggest that either restorational repair is frequent or heteroduplex tracts are shorter in wild-type cells.
T15 1143-1292 DRI_Outcome denotes Comparison of mlh1 segregation data with that of pms1, msh2, msh3, and msh6 mutants show that the ability to promote crossing-over is unique to MLH1.
T16 1293-1475 DRI_Approach denotes Taken together these observations indicate that both crossing-over and gene conversion require MutS and MutL functions and that Mlh1 represents an overlap between these two pathways.
T17 1476-1514 DRI_Background denotes Models of Mlh1 function are discussed.