| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-92 |
DRI_Background |
denotes |
Evidence for involvement of yeast proliferating cell nuclear antigen in DNA mismatch repair. |
| T2 |
93-169 |
DRI_Approach |
denotes |
DNA mismatch repair plays a key role in the maintenance of genetic fidelity. |
| T3 |
170-308 |
DRI_Challenge |
denotes |
Mutations in the human mismatch repair genes hMSH2, hMLH1, hPMS1, and hPMS2 are associated with hereditary nonpolyposis colorectal cancer. |
| T4 |
309-428 |
DRI_Approach |
denotes |
The proliferating cell nuclear antigen (PCNA) is essential for DNA replication, where it acts as a processivity factor. |
| T5 |
429-659 |
DRI_Approach |
denotes |
Here, we identify a point mutation, pol30-104, in the Saccharomyces cerevisiae POL30 gene encoding PCNA that increases the rate of instability of simple repetitive DNA sequences and raises the rate of spontaneous forward mutation. |
| T6 |
660-896 |
DRI_Outcome |
denotes |
Epistasis analyses with mutations in mismatch repair genes MSH2, MLH1, and PMS1 suggest that the pol30-104 mutation impairs MSH2/MLH1/PMS1-dependent mismatch repair, consistent with the hypothesis that PCNA functions in mismatch repair. |
| T7 |
897-1051 |
DRI_Background |
denotes |
MSH2 functions in mismatch repair with either MSH3 or MSH6, and the MSH2-MSH3 and MSH2-MSH6 heterodimers have a role in the recognition of DNA mismatches. |
| T8 |
1052-1154 |
DRI_Outcome |
denotes |
Consistent with the genetic data, we find specific interaction of PCNA with the MSH2-MSH3 heterodimer. |
