UseCases_ArguminSci_Discourse  

PubMed:25605670 JSONTXT 17 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-78 DRI_Background denotes Microfluidic model of ductal carcinoma in situ with 3D, organotypic structure.
T2 88-244 DRI_Background denotes D: Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is thought to be a precursor to most invasive and metastatic breast cancers.
T3 245-398 DRI_Challenge denotes Understanding the mechanisms regulating the invasive transition of DCIS is critical in order to better understand how some types of DCIS become invasive.
T4 399-584 DRI_Background denotes While significant insights have been gained using traditional in vivo and in vitro models, existing models do not adequately recapitulate key structure and functions of human DCIS well.
T5 585-683 DRI_Background denotes In addition, existing models are time-consuming and costly, limiting their use in routine screens.
T6 684-898 DRI_Challenge denotes Here, we present a microscale DCIS model that recapitulates key structures and functions of human DCIS, while enhancing the throughput capability of the system to simultaneously screen numerous molecules and drugs.
T7 908-959 DRI_Approach denotes Our microscale DCIS model is prepared in two steps.
T8 960-1089 DRI_Background denotes First, viscous finger patterning is used to generate mammary epithelial cell-lined lumens through extracellular matrix hydrogels.
T9 1090-1175 DRI_Approach denotes Next, DCIS cells are added to fill the mammary ducts to create a DCIS-like structure.
T10 1176-1318 DRI_Approach denotes For coculture experiments, human mammary fibroblasts (HMF) are added to the two side channels connected to the center channel containing DCIS.
T11 1319-1457 DRI_Outcome denotes To validate the invasive transition of the DCIS model, the invasion of cancer cells and the loss of cell-cell junctions are then examined.
T12 1458-1513 DRI_Unspecified denotes A student t-test is conducted for statistical analysis.
T13 1523-1723 DRI_Outcome denotes We demonstrate that our DCIS model faithfully recapitulates key structures and functions of human mammary DCIS and can be employed to study the mechanisms involved in the invasive progression of DCIS.
T14 1724-1868 DRI_Background denotes First, the formation of cell-cell junctions and cell polarity in the normal mammary duct, and the structure of the DCIS model are characterized.
T15 1869-1936 DRI_Background denotes Second, coculture with HMF is shown to induce the invasion of DCIS.
T16 1937-2013 DRI_Outcome denotes Third, multiple endpoint analyses are demonstrated to validate the invasion.
T17 2027-2154 DRI_Outcome denotes We have developed and characterized a novel in vitro model of normal and DCIS-inflicted mammary ducts with 3D lumen structures.
T18 2155-2302 DRI_Background denotes These models will enable researchers to investigate the role of microenvironmental factors on the invasion of DCIS in more in vivo-like conditions.