UseCases_ArguminSci_Discourse  

PubMed:25406584 JSONTXT 14 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-115 DRI_Background denotes Non-enzymatic, serum-free tissue culture of pre-invasive breast lesions for spontaneous generation of mammospheres.
T2 116-311 DRI_Background denotes Breast ductal carcinoma in situ (DCIS), by definition, is proliferation of neoplastic epithelial cells within the confines of the breast duct, without breaching the collagenous basement membrane.
T3 312-488 DRI_Background denotes While DCIS is a non-obligate precursor to invasive breast cancers, the molecular mechanisms and cell populations that permit progression to invasive cancer are not fully known.
T4 489-730 DRI_Challenge denotes To determine if progenitor cells capable of invasion existed within the DCIS cell population, we developed a methodology for collecting and culturing sterile human breast tissue at the time of surgery, without enzymatic disruption of tissue.
T5 731-874 DRI_Background denotes Sterile breast tissue containing ductal segments is harvested from surgically excised breast tissue following routine pathological examination.
T6 875-1017 DRI_Background denotes Tissue containing DCIS is placed in nutrient rich, antibiotic-containing, serum free medium, and transported to the tissue culture laboratory.
T7 1018-1088 DRI_Approach denotes The breast tissue is further dissected to isolate the calcified areas.
T8 1089-1258 DRI_Background denotes Multiple breast tissue pieces (organoids) are placed in a minimal volume of serum free medium in a flask with a removable lid and cultured in a humidified CO₂ incubator.
T9 1259-1346 DRI_Background denotes Epithelial and fibroblast cell populations emerge from the organoid after 10 - 14 days.
T10 1347-1423 DRI_Background denotes Mammospheres spontaneously form on and around the epithelial cell monolayer.
T11 1424-1528 DRI_Approach denotes Specific cell populations can be harvested directly from the flask without disrupting neighboring cells.
T12 1529-1668 DRI_Outcome denotes Our non-enzymatic tissue culture system reliably reveals cytogenetically abnormal, invasive progenitor cells from fresh human DCIS lesions.