UseCases_ArguminSci_Discourse  

PubMed:24550389 JSONTXT 29 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-133 DRI_Background denotes Mispair-specific recruitment of the Mlh1-Pms1 complex identifies repair substrates of the Saccharomyces cerevisiae Msh2-Msh3 complex.
T2 134-197 DRI_Approach denotes DNA mismatch repair is initiated by either the Msh2-Msh6 or the
T3 198-215 Token_Label.OUTSIDE denotes Msh2-Msh3 mispair
T4 216-240 DRI_Approach denotes recognition heterodimer.
T5 241-462 DRI_Approach denotes Here we optimized the expression and purification of Saccharomyces cerevisiae Msh2-Msh3 and performed a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment.
T6 463-730 DRI_Approach denotes Msh2-Msh3 formed sliding clamps and recruited Mlh1-Pms1 on +1, +2, +3, and +4 insertion/deletions and CC, AA, and possibly GG mispairs, whereas Msh2-Msh6 formed mispair-dependent sliding clamps and recruited Mlh1-Pms1 on 7 of the 8 possible base:base mispairs, the +1
T7 731-757 Token_Label.OUTSIDE denotes insertion/deletion mispair
T8 757-808 DRI_Approach denotes , and to a low level on the +2 but not the +3 or +4
T9 809-836 Token_Label.OUTSIDE denotes insertion/deletion mispairs
T10 837-863 DRI_Approach denotes and not on the CC mispair.
T11 864-1094 DRI_Challenge denotes The mispair specificity of sliding clamp formation and Mlh1-Pms1 recruitment but not mispair binding alone correlated best with genetic data on the mispair specificity of Msh2-Msh3- and Msh2-Msh6-dependent mismatch repair in vivo.
T12 1095-1501 DRI_Approach denotes Analysis of an Msh2-Msh6/Msh3 chimeric protein and mutant Msh2-Msh3 complexes showed that the nucleotide binding domain and communicating regions but not the mispair binding domain of Msh2-Msh3 are responsible for the extremely rapid dissociation of Msh2-Msh3 sliding clamps from DNA relative to that seen for Msh2-Msh6, and that amino acid residues predicted to stabilize Msh2-Msh3 interactions with bent,
T13 1502-1537 Token_Label.OUTSIDE denotes strand-separated mispair-containing
T14 1538-1647 DRI_Approach denotes DNA are more critical for the recognition of small +1 insertion/deletions than larger +4 insertion/deletions.