| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-102 |
DRI_Approach |
denotes |
Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease. |
| T2 |
103-295 |
DRI_Approach |
denotes |
Crossing over between homologous chromosomes is initiated in meiotic prophase in most sexually reproducing organisms by the appearance of programmed double strand breaks throughout the genome. |
| T3 |
296-469 |
DRI_Approach |
denotes |
In Saccharomyces cerevisiae the double-strand breaks are resected to form three prime single-strand tails that primarily invade complementary sequences in unbroken homologs. |
| T4 |
470-631 |
DRI_Approach |
denotes |
These invasion intermediates are converted into double Holliday junctions and then resolved into crossovers that facilitate homolog segregation during Meiosis I. |
| T5 |
632-899 |
DRI_Approach |
denotes |
Work in yeast suggests that Msh4-Msh5 stabilizes invasion intermediates and double Holliday junctions, which are resolved into crossovers in steps requiring Sgs1 helicase, Exo1, and a putative endonuclease activity encoded by the DNA mismatch repair factor Mlh1-Mlh3. |
| T6 |
900-1051 |
DRI_Approach |
denotes |
We purified Mlh1-Mlh3 and showed that it is a metal-dependent and Msh2-Msh3-stimulated endonuclease that makes single-strand breaks in supercoiled DNA. |
| T7 |
1052-1200 |
DRI_Challenge |
denotes |
These observations support a direct role for an Mlh1-Mlh3 endonuclease activity in resolving recombination intermediates and in DNA mismatch repair. |
