UseCases_ArguminSci_Discourse  

PubMed:22813865 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
T1 0-118 DRI_Challenge denotes Spatiotemporal evolution of blood brain barrier damage and tissue infarction within the first 3h after ischemia onset.
T2 119-326 DRI_Outcome denotes Blood brain barrier (BBB) damage that occurs within the thrombolytic time window is increasingly appreciated to negatively impact the safety and efficacy profiles of thrombolytic therapy for ischemic stroke.
T3 327-451 DRI_Challenge denotes However, the spatiotemporal evolution of BBB damage in this early stroke stage and the underlying mechanisms remain unclear.
T4 452-668 DRI_Approach denotes Here, we investigated the topographical distribution of BBB damage and its association with tissue injury within the first 3 h after ischemia onset and the roles of matrix metalloproteinase (MMP)-2/9 in this process.
T5 669-840 DRI_Background denotes Rats were subjected to 1, 2, or 3 h of middle cerebral artery occlusion (MCAO) followed by 10 min reperfusion with fluorescence-labeled dextran as BBB permeability marker.
T6 841-939 DRI_Background denotes Acute tissue infarction was evidenced by staining defect with triphenyltetrazolium chloride (TTC).
T7 940-1009 DRI_Background denotes Cerebral blood flow (CBF) was measured by magnetic resonance imaging.
T8 1010-1062 DRI_Background denotes MMP-2/9 were assessed by gel and in situ zymography.
T9 1063-1068 DRI_Unspecified denotes After
T10 1069-1077 Token_Label.OUTSIDE denotes 2-h MCAO
T11 1077-1257 DRI_Unspecified denotes , dextran leakage was seen in the ischemic ventromedial striatum and the preoptic area which showed ~70% CBF reduction, and expanded to other MCA regions including the cortex after
T12 1258-1266 Token_Label.OUTSIDE denotes 3-h MCAO
T13 1266-1267 DRI_Unspecified denotes .
T14 1268-1386 DRI_Background denotes Interestingly, high (2000 kDa) and low (70 kDa) molecular weight dextrans displayed almost identical leakage patterns.
T15 1387-1548 DRI_Outcome denotes Different from BBB damage, tissue infarction was first seen in the ischemic dorsal striatum and the parietal/insular cortex which experienced ~90% CBF reduction.
T16 1549-1690 DRI_Background denotes Increased gelatinolytic activity colocalized with dextran leakage, and MMP-2 was found to be the major enzymatic source on gelatin zymograms.
T17 1691-1786 DRI_Approach denotes Pretreatment with MMP inhibitor GM6001 significantly reduced dextran leakage induced by 2-h and
T18 1787-1795 Token_Label.OUTSIDE denotes 3-h MCAO
T19 1795-1796 DRI_Approach denotes .
T20 1797-1963 DRI_Outcome denotes Taken together, our findings reveal substantial differences in the topographic distribution of BBB damage and tissue infarction within the first 3 h after MCAO onset.
T21 1964-2140 DRI_Challenge denotes Unlike ischemic neuronal damage, BBB damage appears to develop faster in brain regions with moderately severe ischemia, and MMP-2 contributes to this early ischemic BBB damage.