UseCases_ArguminSci_Discourse  

PubMed:19735549 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-100 DRI_Background denotes An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ.
T2 115-253 DRI_Background denotes Human models of noninvasive breast tumors are limited, and the existing in vivo models do not mimic inter- and intratumoral heterogeneity.
T3 254-346 DRI_Background denotes Ductal carcinoma in situ (DCIS) is the most common type (80%) of noninvasive breast lesions.
T4 347-479 DRI_Background denotes The aim of this study was to develop an in vivo model whereby the natural progression of human DCIS might be reproduced and studied.
T5 480-578 DRI_Approach denotes To accomplish this goal, the intraductal human-in-mouse (HIM) transplantation model was developed.
T6 579-796 DRI_Outcome denotes The resulting models, which mimicked some of the diversity of human noninvasive breast cancers in vivo, were used to show whether subtypes of human DCIS might contain distinct subpopulations of tumor-initiating cells.
T7 806-1039 DRI_Background denotes The intraductal models were established by injection of human DCIS cell lines (MCF10DCIS.COM and SUM-225), as well as cells derived from a primary human DCIS (FSK-H7), directly into the primary mouse mammary ducts via cleaved nipple.
T8 1040-1227 DRI_Approach denotes Six to eight weeks after injections, whole-mount, hematoxylin and eosin, and immunofluorescence staining were performed to evaluate the type and extent of growth of the DCIS-like lesions.
T9 1228-1459 DRI_Approach denotes To identify tumor-initiating cells, putative human breast stem/progenitor subpopulations were sorted from MCF10DCIS.COM and SUM-225 with flow cytometry, and their in vivo growth fractions were compared with the Fisher's Exact test.
T10 1469-1593 DRI_Background denotes Human DCIS cells initially grew within the mammary ducts, followed by progression to invasion in some cases into the stroma.
T11 1594-1675 DRI_Outcome denotes The lesions were histologically almost identical to those of clinical human DCIS.
T12 1676-1800 DRI_Background denotes This method was successful for growing DCIS cell lines (MCF10DCIS.COM and SUM-225) as well as a primary human DCIS (FSK-H7).
T13 1801-1913 DRI_Background denotes MCF10DCIS.COM represented a basal-like DCIS model, whereas SUM-225 and FSK-H7 cells were models for HER-2+ DCIS.
T14 1914-2050 DRI_Outcome denotes With this approach, we showed that various subtypes of human DCIS appeared to contain distinct subpopulations of tumor-initiating cells.
T15 2064-2299 DRI_Outcome denotes The intraductal HIM transplantation model provides an invaluable tool that mimics human breast heterogeneity at the noninvasive stages and allows the study of the distinct molecular and cellular mechanisms of breast cancer progression.