UseCases_ArguminSci_Discourse  

PubMed:18787417 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-78 DRI_Background denotes Comedo-ductal carcinoma in situ: A paradoxical role for programmed cell death.
T2 79-264 DRI_Challenge denotes Comedo-DCIS is a histologic subtype of preinvasive breast neoplasia that is characterized by prominent apoptotic cell death and has greater malignant potential than other DCIS subtypes.
T3 265-385 DRI_Challenge denotes We investigated the mechanisms of apoptosis in comedo-DCIS and its role in conversion of comedo-DCIS to invasive cancer.
T4 386-525 DRI_Background denotes Clinical comedo-DCIS excisions and the MCF10DCIS.com human breast cancer model which produces lesions resembling comedo-DCIS were analyzed.
T5 526-721 DRI_Background denotes Apoptotic luminal and myoepithelial cells were identified by TUNEL and reactivity to cleaved PARP antibody and cell death assessed by Western blotting, Mitocapture and immunohistochemical assays.
T6 722-941 DRI_Background denotes MCF10DCIS.com cells undergo spontaneous apoptosis in vitro, both in monolayers and multicellular spheroids; it is associated with increased mitochondrial membrane permeability, increase in Bax/Bcl-2 ratio and occurs via
T7 942-977 Token_Label.OUTSIDE denotes caspase-9-dependent p53-independent
T8 978-986 DRI_Background denotes pathway.
T9 987-1094 DRI_Background denotes This suggests that apoptosis is stromal-independent and that the cells are programmed to undergo apoptosis.
T10 1095-1282 DRI_Outcome denotes Immunostaining with cleaved PARP antibody showed that myoepithelial apoptosis occurs before lesions progress to comedo-DCIS in both clinical comedo-DCIS and in vivo MCF10DCIS.com lesions.
T11 1283-1471 DRI_Background denotes Intense staining for MMP-2, MMP-3, MMP-9 and MMP-11 was observed in the stroma and epithelia of solid DCIS lesions prior to conversion to comedo-DCIS in clinical and MCF10DCIS.com lesions.
T12 1472-1564 DRI_Unspecified denotes Gelatin zymography showed higher MMP-2 levels in lysates and conditioned media of MCF10DCIS.
T13 1565-1596 DRI_Unspecified denotes com cells undergoing apoptosis.
T14 1597-1811 DRI_Outcome denotes These data suggest that signals arising from the outside (microenvironmental) and inside (internal genetic alterations) of the duct act in concert to trigger apoptosis of myoepithelial and luminal epithelial cells.
T15 1812-1909 DRI_Outcome denotes Our findings implicate spontaneous apoptosis in both the etiology and progression of comedo-DCIS.
T16 1910-2100 DRI_Challenge denotes It is possible that spontaneous apoptosis facilitates elimination of cells thus permitting expansion and malignant transformation of cancer cells that are resistant to spontaneous apoptosis.