UseCases_ArguminSci_Discourse  

PubMed:18182054 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T1 0-96 DRI_Background denotes Role of autophagy in G2019S-LRRK2-associated neurite shortening in differentiated SH-SY5Y cells.
T2 97-333 DRI_Challenge denotes Neuritic retraction represents a prominent feature of the degenerative phenotype associated with mutations in leucine rich repeat kinase 2 (LRRK2) that are implicated in autosomal dominant and some cases of sporadic Parkinson's disease.
T3 334-461 DRI_Background denotes Alterations in macroautophagy, the vacuolar catabolism of cytoplasmic constituents, have been described in Parkinson's disease.
T4 462-623 DRI_Approach denotes In this study, we utilized retinoic-acid differentiated SH-SY5Y cells to determine whether autophagy contributes to mutant LRRK2-associated neurite degeneration.
T5 624-639 DRI_Background denotes Transfection of
T6 640-666 Token_Label.OUTSIDE denotes pre-differentiated SH-SY5Y
T7 667-852 DRI_Background denotes cells with LRRK2 cDNA containing the common G2019S mutation resulted in significant decreases in neurite length, which were not observed in cells transfected with wild type LRRK2 or its
T8 853-871 Token_Label.OUTSIDE denotes kinase-dead K1906M
T9 872-881 DRI_Background denotes mutation.
T10 882-888 REPLACED denotes G2019S
T11 889-894 Token_Label.OUTSIDE denotes LRRK2
T12 895-1213 DRI_Outcome denotes transfected cells also exhibited striking increases in autophagic vacuoles in both neuritic and somatic compartments, as demonstrated by fluorescence and western blot analysis of the autophagy marker green fluorescent protein-tagged microtubule-associated protein Light Chain 3 and by transmission electron microscopy.
T13 1214-1343 DRI_Background denotes RNA interference knockdown of LC3 or Atg7, two essential components of the conserved autophagy machinery, reversed the effects of
T14 1344-1356 Token_Label.OUTSIDE denotes G2019S LRRK2
T15 1357-1440 DRI_Background denotes expression on neuronal process length, whereas rapamycin potentiated these effects.
T16 1441-1633 DRI_Background denotes The mitogen activated protein kinase/extracellular signal regulated protein kinase (MAPK/ERK) kinase (MEK) inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126) reduced
T17 1634-1656 Token_Label.OUTSIDE denotes LRRK2-induced neuritic
T18 1657-1730 DRI_Background denotes autophagy and neurite shortening, implicating MAPK/ERK-related signaling.
T19 1731-1845 DRI_Background denotes These results indicate an active role for autophagy in neurite remodeling induced by pathogenic mutation of LRRK2.