UseCases_ArguminSci_Discourse  

PubMed:18057311 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-121 DRI_Background denotes Comparative analysis of meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway.
T2 122-242 DRI_Background denotes The DNA mismatch repair (MMR) family functions in a variety of contexts to preserve genome integrity in most eukaryotes.
T3 243-351 DRI_Background denotes In particular, members of the MMR family are involved in the process of meiotic recombination in germ cells.
T4 352-509 DRI_Outcome denotes MMR gene mutations in mice result in meiotic disruption during prophase I, but the extent of this disruption often differs between male and female meiocytes.
T5 510-792 DRI_Approach denotes To address the role of MMR proteins specifically in female meiosis, we explored the progression of oocytes through prophase I and the meiotic divisions in mice harboring deletions in members of the MMR pathway (Mlh1, Mlh3, Exo1, and an ATPase-deficient variant of Mlh1, Mlh1(G67R)).
T6 793-1028 DRI_Outcome denotes The colocalization of MLH1 and MLH3, key proteins involved in stabilization of nascent crossovers, was dependent on intact heterodimer formation and was highly correlated with the ability of oocytes to progress through to metaphase II.
T7 1029-1165 DRI_Background denotes The exception was Exo1(-/-) oocytes, in which normal MLH1/MLH3 localization was observed followed by failure to proceed to metaphase II.
T8 1166-1368 DRI_Background denotes All mutant oocytes were able to resume meiosis after dictyate arrest, but they showed a dramatic decline in chiasmata (to less than 25% of normal), accompanied by varied progression through metaphase I.
T9 1369-1767 DRI_Outcome denotes Taken together, these results demonstrate that MMR function is required for the formation and stabilization of crossovers in mammalian oocytes and that, in the absence of a functional MMR system, the failure to maintain chiasmata results in a reduced ability to proceed normally through the first and second meiotic divisions, despite near-normal levels of meiotic resumption after dictyate arrest.