UseCases_ArguminSci_Discourse  

PubMed:17156757 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-118 DRI_Background denotes Peripheral thermal injury causes blood-brain barrier dysfunction and matrix metalloproteinase (MMP) expression in rat.
T2 119-293 DRI_Challenge denotes Mortality after serious systemic thermal injury may be linked to significant increases in cerebral vascular permeability and edema due to blood-brain barrier (BBB) breakdown.
T3 294-417 DRI_Challenge denotes This BBB disruption is thought to be mediated by a family of proteolytic enzymes known as matrix metalloproteinases (MMPs).
T4 418-549 DRI_Background denotes The gelatinases, MMP-2 and MMP-9, digest the endothelial basal lamina of the BBB, which is essential for maintaining BBB integrity.
T5 550-711 DRI_Challenge denotes The current study investigated whether disruption of microvascular integrity in a rat thermal injury model is associated with gelatinase expression and activity.
T6 712-957 DRI_Approach denotes Seventy-two adult Sprague-Dawley rats were anesthetized and submerged horizontally, in the supine position, in 100 degrees C (37 degrees C for controls) water for 6 s producing a third-degree burn affecting 60-70% of the total body surface area.
T7 958-1012 DRI_Background denotes Brain edema was detected by calculating water content.
T8 1013-1125 DRI_Background denotes Real time PCR, Western blot, and zymography were used to quantify MMP mRNA, protein, and enzyme activity levels.
T9 1126-1194 DRI_Approach denotes Each group was quantified at 3, 7, 24, and 72 h post thermal injury.
T10 1195-1269 DRI_Background denotes Brain water content was significantly increased 7 through 72 h after burn.
T11 1270-1591 DRI_Unspecified denotes Expression of brain MMP-9 mRNA was significantly increased as early as 3 h after thermal injury compared to controls, remained at 7 h (p<0.01), and returned to control levels by 24 h. MMP-9 protein levels and enzyme activity began to increase at 7 h and reached significant levels between 7 and 24 h after thermal injury.
T12 1592-1711 DRI_Background denotes While MMP-9 protein levels continued to increase significantly through 72 h, enzyme activity returned to control level.
T13 1712-1920 DRI_Challenge denotes The increase in MMP-9 expression and activity, associated with increased BBB permeability following thermal injury, indicates that MMP-9 may contribute to observed cerebral edema in peripheral thermal injury.