UseCases_ArguminSci_Discourse  

PubMed:16212944 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-122 DRI_Outcome denotes 17beta-Estradiol attenuates blood-brain barrier disruption induced by cerebral ischemia-reperfusion injury in female rats.
T2 123-260 DRI_Challenge denotes Disruption of blood-brain barrier (BBB), mediated through matrix metalloproteinases (MMPs), is a critical event during cerebral ischemia.
T3 261-424 DRI_Background denotes While neuroprotective effects of estrogens have been well established in ischemic stroke models, the effects of estrogens on BBB integrity remain to be elucidated.
T4 425-601 DRI_Challenge denotes In the present study, we determined effects of 17beta-estradiol (E2) on BBB disruption induced by transient focal cerebral ischemia and its effects on MMP2 and MMP9 activation.
T5 602-739 DRI_Background denotes Transient cerebral ischemia was induced by middle cerebral artery (MCA) occlusion for 1 h followed by reperfusion in ovariectomized rats.
T6 740-812 DRI_Background denotes E2 (100 microg/kg) or vehicle was administered 2 h before MCA occlusion.
T7 813-894 DRI_Approach denotes BBB integrity was determined by fluorescent detection of extravasated Evans blue.
T8 895-1029 DRI_Background denotes In separate experiments, effect of E2 on MMP2 and MMP9 expression and activation was determined by immunoblot and MMPs activity assay.
T9 1030-1169 DRI_Background denotes E2 treatment prevented more than 50% and 30% of BBB disruption in the ischemic cortex and subcortex at 4 h after reperfusion, respectively.
T10 1170-1318 DRI_Background denotes MMP2 and MMP9 expression was elevated at 2 h and peaked at 4 h after reperfusion in the ischemic cortex, which was markedly reduced by E2 treatment.
T11 1319-1417 DRI_Background denotes E2 treatment also attenuated the increase of MMPs activity induced by ischemia-reperfusion injury.
T12 1418-1556 DRI_Challenge denotes In conclusion, estrogens could attenuate BBB disruption induced by transient cerebral ischemia, by inhibition of MMP2 and MMP9 activation.
T13 1557-1735 DRI_Outcome denotes Our results suggest an important role of estrogens as multiple targeting protectants against ischemic stroke on cellular as well as vascular components of central nervous system.