UseCases_ArguminSci_Discourse  

PubMed:16177809 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-192 DRI_Background denotes Atorvastatin downregulates tissue plasminogen activator-aggravated genes mediating coagulation and vascular permeability in single cerebral endothelial cells captured by laser microdissection.
T2 193-319 DRI_Background denotes The effects of statins on gene expression of cerebral endothelial cells (ECs) in vivo have not been investigated after stroke.
T3 320-602 DRI_Challenge denotes We developed a rapid double immunofluorescent staining protocol with antibodies against von Willebrand factor (a marker for endothelium) and glial fibrillary acidic protein (a marker for astrocytes) for laser capture microdissection to isolate single ECs in brain tissue of the rat.
T4 603-865 DRI_Outcome denotes Using this protocol in combination with real-time PCR, we found that stroke significantly increased mRNA levels of protease-activated receptor 1 (PAR-1) and tissue factor (TF) in ECs isolated from ischemic cerebral microvessels compared with nonischemic vessels.
T5 866-1193 DRI_Background denotes Treatment of embolic stroke with recombinant human tissue plasminogen activator (rht-PA) 4 h after stroke further elevated PAR-1 mRNA levels nearly 1000-fold in the core and 500-fold in the boundary above the nonstroke group 30 h after stroke, while TF mRNA levels were elevated approximately 10 fold above the nonstroke group.
T6 1194-1355 DRI_Background denotes Furthermore, stroke significantly increased matrix metalloproteinase (MMP) 2 and 9 mRNA levels in the ischemic core and boundary regions 6 and 30 h after stroke.
T7 1356-1370 DRI_Background denotes Treatment with
T8 1371-1394 Token_Label.OUTSIDE denotes rht-PA-upregulated MMP2
T9 1395-1440 DRI_Background denotes expression in the ischemic boundary and core.
T10 1441-1592 DRI_Background denotes Atorvastatin completely blocked rht-PA upregulation of the above genes, when atorvastatin in combination with rht-PA was administered 4 h after stroke.
T11 1593-1717 DRI_Background denotes Monotherapy of atorvastatin 4 h after stroke did not significantly reduce expression of genes examined in the present study.
T12 1718-1988 DRI_Challenge denotes These data provide evidence that atorvastatin reduces exogenous tPA-aggravated cerebral endothelial genes that mediate thrombosis and blood-brain barrier permeability, which could contribute to the beneficial effects of statins on thrombolytic treatment of acute stroke.