UseCases_ArguminSci_Discourse  

PubMed:15870843 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-94 DRI_Approach denotes Ischemia-reperfusion injury stimulates gelatinase expression and activity in kidney glomeruli.
T2 95-304 DRI_Challenge denotes Although ischemia remains the leading cause of acute renal failure in humans, there is little information on the expression and activities of gelatinases of kidney glomeruli during ischemia-reperfusion injury.
T3 305-461 DRI_Approach denotes In this study, we used a unilateral ischemia-reperfusion model to investigate the activity and expression of gelatinases in glomeruli during acute ischemia.
T4 462-592 DRI_Background denotes Unilateral ischemia was induced in rats by vascular clamping (30 min) followed by reperfusion (60 min) and isolation of glomeruli.
T5 593-703 DRI_Background denotes The activity and expression of gelatinase proteins were determined by gelatin zymography and Western blotting.
T6 704-771 DRI_Unspecified denotes Gelatinase mRNA levels were evaluated by reverse transcriptase-PCR.
T7 772-864 DRI_Background denotes Ischemia and reperfusion increased serum creatinine levels, hallmark of acute renal failure.
T8 865-916 DRI_Background denotes Ischemia induced mRNA and protein MMP-2 expression.
T9 917-1017 DRI_Outcome denotes There was strong stimulation of MMP-9 mRNA, both forms of dimeric MMP-9, and active monomeric MMP-9.
T10 1018-1102 DRI_Background denotes In contrast to TIMP-1 decreasing, TIMP-2 protein and mRNA increased during ischemia.
T11 1103-1258 DRI_Background denotes During reperfusion, there was a gradual reversal of the MMP-2 and MMP-9 levels and a strong inhibition of TIMP-1 and TIMP-2 at the protein and mRNA levels.
T12 1259-1354 DRI_Background denotes Endocytic receptor LRP was increased during ischemia and returned to normal during reperfusion.
T13 1355-1431 DRI_Outcome denotes Expression of MMP-9 docking receptor CD-44 was increased during reperfusion.
T14 1432-1569 DRI_Background denotes Finally, ZO-1, an in vivo MMP-9 substrate, was degraded during ischemia, revealing that MMP-9 upregulated during ischemia was functional.
T15 1570-1749 DRI_Outcome denotes Our data suggest that stimulation of gelatinase activity during ischemia could contribute to glomeruli injury, providing new therapeutic targets for acute renal failure in humans.
T16 1750-1878 DRI_Background denotes In contrast, elevated monomeric MMP-9 activity due to TIMP-1 decrease during reperfusion may participate to glomerular recovery.