| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T4 |
0-105 |
DRI_Background |
denotes |
Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin. |
| T5 |
106-223 |
DRI_Background |
denotes |
MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence. |
| T6 |
224-345 |
DRI_Approach |
denotes |
Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2). |
| T7 |
346-480 |
DRI_Background |
denotes |
Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR. |
| T8 |
481-529 |
DRI_Background |
denotes |
Protein expression was assessed by Western blot. |
| T9 |
530-589 |
DRI_Unspecified |
denotes |
MMP-2 and -9 activity was determined by gelatin zymography. |
| T10 |
590-635 |
DRI_Background |
denotes |
Active MT1-MMP levels were measured by ELISA. |
