UseCases_ArguminSci_Discourse  

PubMed:15240428 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-94 DRI_Background denotes Matrix metalloproteinases and their tissue inhibitors in the developing neonatal mouse uterus.
T2 95-230 DRI_Background denotes Postnatal development of the mouse uterus involves differentiation and development of the endometrial glands as well as the myometrium.
T3 231-401 DRI_Background denotes Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in extracellular matrix breakdown and morphogenesis of many epitheliomesenchymal organs.
T4 402-616 DRI_Outcome denotes As a first step to understanding their roles in postnatal mouse uterine development, MMPs and TIMPs found to be expressed in the neonatal mouse uterus by microarray analysis were localized by in situ hybridization.
T5 617-776 DRI_Outcome denotes The MMP-2 mRNA was detected only in the uterine stroma, whereas the MMP-10 mRNA was present only in the uterine epithelium from Postnatal Day (PND) 3 to PND 9.
T6 777-956 DRI_Outcome denotes All other MMPs (MMP-11, MMP-14, and MMP-23) as well as TIMP-1, TIMP-2, and TIMP-3 were detected in both epithelial and stromal cells of the endometrium, but not in the myometrium.
T7 957-1090 DRI_Background denotes Uterine extracts were then analyzed by gelatin and casein gel zymography to detect active gelatinases and stromelysins, respectively.
T8 1091-1256 DRI_Background denotes Five major gelatinase bands of activity were detected and inhibited by the MMP inhibitors, EDTA or 1,10-phenanthroline, but not by PMSF, a serine protease inhibitor.
T9 1257-1344 DRI_Background denotes Western blot analysis confirmed the presence of MMP-2 and MMP-9 proteins in the uterus.
T10 1345-1359 REPLACED denotes Immunoreactive
T11 1360-1365 Token_Label.OUTSIDE denotes MMP-9
T12 1366-1426 DRI_Outcome denotes protein was detected only in the endometrial stroma, whereas
T13 1427-1447 Token_Label.OUTSIDE denotes immunoreactive MMP-2
T14 1448-1517 DRI_Outcome denotes protein was detected in both the stroma and epithelium of the uterus.
T15 1518-1774 DRI_Background denotes Casein zymography detected three major bands of activity ( approximately 54, 63, and 80 kDa) that were inhibited by the serine protease inhibitor, PMSF, but not by the MMP inhibitors, EDTA or 1,10-phenanthroline, suggesting that they were serine proteases.
T16 1775-1883 DRI_Outcome denotes These results support the hypothesis that MMPs and TIMPs regulate postnatal development of the mouse uterus.