| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-185 |
DRI_Background |
denotes |
Herbal medicine Sho-saiko-to (TJ-9) increases expression matrix metalloproteinases (MMPs) with reduced expression of tissue inhibitor of metalloproteinases (TIMPs) in rat stellate cell. |
| T2 |
186-260 |
DRI_Approach |
denotes |
We have reported that Sho-saiko-to (TJ-9) prevents liver fibrosis in vivo. |
| T3 |
261-417 |
DRI_Approach |
denotes |
To gain further insights into the effect of TJ-9, the matrix metalloproteinases (MMPs)/tissue inhibitors of metalloproteinases (TIMPs) balance was examined. |
| T4 |
418-564 |
DRI_Background |
denotes |
Hepatic stellate cells (HSCs) were isolated from male Wistar rats and cultured with TJ-9 (0-1000 microg/ml) on uncoated plastic dishes for 4 days. |
| T5 |
565-746 |
DRI_Approach |
denotes |
To elucidate the effects on the MMPs/TIMPs balance by TJ-9, quantitative analysis of type IV collagen-degrading activity, gelatin zymography and reverse zymography were carried out. |
| T6 |
747-842 |
DRI_Approach |
denotes |
Northern blot analysis was performed to determine the expression of MMP-2, 13 and TIMP-1 mRNAs. |
| T7 |
843-970 |
DRI_Background |
denotes |
TJ-9 treatment resulted in dose-dependent upregulation of MMP-2, 13 mRNA and downregulation of TIMP-1 mRNA up to 500 microg/ml. |
| T8 |
971-1182 |
DRI_Background |
denotes |
Gelatin zymography, reverse zymography and quantitative analysis of type IV collagen-degrading activity confirmed that TJ-9 increased MMP-2 activity and prevented TIMP-1, 2 activities in a dose-dependent manner. |
| T9 |
1183-1322 |
DRI_Outcome |
denotes |
SB203580 diminished the reduction of mRNA as well as the activity of TIMP-1 by TJ-9 and induction of mRNA as well as the activity of MMP-2. |
| T10 |
1323-1448 |
DRI_Background |
denotes |
These results show that TJ-9 increased MMP-2, 13 activity with reduced TIMP-1, 2 activities on HSCs possibly via P38 pathway. |
