UseCases_ArguminSci_Discourse  

PubMed:14662518 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-96 DRI_Background denotes Anti-HIV drugs decrease the expression of matrix metalloproteinases in astrocytes and microglia.
T2 97-306 DRI_Challenge denotes The introduction of potent antiretroviral drugs for the treatment of patients with human immunodeficiency virus (HIV) infection has dramatically reduced the prevalence of HIV-associated neurological disorders.
T3 307-365 DRI_Challenge denotes Such diseases can be mediated by proteolytic enzymes, i.e.
T4 366-457 DRI_Unspecified denotes matrix metalloproteinases (MMPs) and, in particular gelatinases, released from glial cells.
T5 458-656 DRI_Background denotes The aim of this study was to investigate whether the antiretroviral drugs commonly used for the treatment of HIV-infected patients modulate the activity of MMPs in astrocyte and microglial cultures.
T6 657-856 DRI_Background denotes Primary cultures of rat astrocyte and microglia were treated with different doses of zidovudine (AZT) or indinavir (IDV) for 20 h and simultaneously activated by exposure to lipopolysaccharide (LPS).
T7 857-1086 DRI_Background denotes Culture supernatants collected from astrocytes and microglia after 24 h incubation were subjected to gelatin zymography and western blot analysis for the assessment of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) protein levels.
T8 1087-1227 DRI_Background denotes Total RNA was extracted from glial cells and used for reverse transcriptase-polymerase chain reaction for the assessment of mRNA expression.
T9 1228-1336 DRI_Outcome denotes Our results indicate that both astrocyte and microglial cells constitutively express MMP-2 mRNA and protein.
T10 1337-1438 DRI_Background denotes LPS treatment increased MMP-2 mRNA and protein expression in astrocytes, but not in microglial cells.
T11 1439-1586 DRI_Outcome denotes The treatment with both AZT and IDV dose-dependently inhibited the expression of MMP-2 in astrocytes, whereas it had no effect on microglial cells.
T12 1587-1776 DRI_Background denotes The expression of MMP-9 in both astrocytes and microglia was induced by LPS treatment and was dose-dependently inhibited by AZT and IDV treatment in LPS-stimulated astrocytes and microglia.
T13 1777-2038 DRI_Background denotes These results raise the possibility that AZT and IDV interfere directly with MMP production in glial cells and independently from their antiviral activity, thus suggesting the possible therapeutical use in neurological diseases associated with MMPs involvement.